KR20010044063A - the cultivation method of earthworm - Google Patents

Abstract

PURPOSE: A method for raising earthworm with humus feed is provided, to get good yield of earthworm and to maximize titer of enzyme capable of dissolving thrombi in the body fluids of the earthworm. CONSTITUTION: The feed for raising the earthworm is manufactured by mixing carbon groups containing abundant carbon such as rice bran, saw dust and molasses, and nitrogen groups containing abundant nitrogen such as night soil, waste material of processed meat and residues of animal with inorganic groups such as acid soil, clay and diatomite to contain carbon and nitrogen mixed in a ratio of 15:1-50:1, and then corroding the mixture at a temperature of 0.1-34deg.C and a humidity of 60-80%. The inorganic groups are added for promoting digestion of the earthworm and providing mineral.

Description

The cultivation method of earthworm}

The present invention relates to an earthworm aquaculture method, and more particularly, the titer of an enzyme having a variety of active functions in the earthworm body fluid as well as an excellent earthquake survival rate and an excellent rearing state in the earthworm culture process. It is about the earthworm farming method to maximize the

An earthworm is a herbaceous animal that has a magnetic organ and a male organ in the form of an invertebrate annulus called a tortoise, a terrestrial or a white rhinoceros, and lives by ingesting bushy organic matter in the fertile wetland soils of various regions.

In particular, according to medical texts such as Dongbogam and herbaceous wood, earthworms contain medicinal ingredients, which are effective for hemolysis and fever, as well as for insecticides, fever, luminescence, jaundice, seasonal infectious diseases, and sore throat. Doing.

This is due to the presence of several factors in the body fluids of the earthworm, such as hemolytic factor, complement like factor, lytic factor, agglutinin, and bacteriostatic factor. In particular, it is well known that these factors show strong activity even at very low levels.

Accordingly, many studies are being conducted on extracting the active ingredient from the body fluids of the earthworm for various purposes, or extracting a component that dissolves the thrombi contained in the body fluid of the earthworm, that is, a substance showing thrombolytic activity. .

In general, a blood clot refers to a clot in which blood is coagulated, which is composed of fibers and proteins, and the blood clots are deposited or aggregated in blood vessels, which interferes with blood circulation. Myocardial infarction, hypertension, arteriosclerosis, etc. will cause various heart diseases.

The thrombolytic agent dissolves the thrombi so that blood circulation can be smoothly performed. The lysis mechanism is that the fibrinase in the thrombolytic agent first breaks down the fiber, and then the proteolytic enzyme in the thrombolytic agent decomposes the protein. It will dissolve blood clots.

As described above, since an active ingredient having thrombolytic activity is contained in the earthworm body fluid, many methods are known for effectively extracting body fluid while maximizing its titer during body fluid extraction.

The general method of extracting the body fluid containing the active ingredient from the earthworm has been to first extract the powder containing the active ingredient by wet grinding the earthworm, separating the pulverized product by centrifugation, taking the supernatant and lyophilizing.

As a part of the method for extracting body fluid containing the above-mentioned effective ingredient, the method for preparing gut extracts of Korean Patent Application No. 92-12244, wet grinding the removed gut, and warming the wet grinding material of the sphere, It is known to prepare a powdery oocyte extract which exhibits thrombolytic activity by freeze vacuum drying.

In Korean Patent Application No. 94-7754, "Method of producing gut extract", the gut is mixed with water to self-decompose or pulverized with a homomixer. The pulverized product is filtered to add organic or inorganic acid to the filtrate to precipitate impurities. By drying ethanol to the supernatant, the separated precipitate is dried to remove various impurities present in the stain, and thus, there is known a method for extracting dry powder of stain, which has no odor or odor, shows no hemolysis, and shows high thrombolytic activity.

However, the above-mentioned methods of extracting the spermicide powder having thrombolytic activity simply provide a method of extracting bodily fluid having thrombolytic activity present in the earthworm, and do not provide accurate earthworm farming conditions and methods. Research on earthworm farming is ongoing.

As part of the earthworm culture method, the 'earthworm food using seaweed waste' of Korean Patent Application No. 99-042448, extracts alginic acid from seaweed waste and mixes the manure sludge with the remaining dialginate to remove the microorganisms contained in the manure. There is a known technique for promoting alginate corrosion and using it as an earthworm food.

In addition, Korean Patent Application No. 97-017930, 'Methods and methods for collecting earthworms and fecal soil using food wastes,' in the lower half by the fecal discharge means installed in the middle of the culture tank, and the space of the upper half partitioned Putting seedworms into the ground; Injecting food waste into the upper layer of the earthworm; Increasing the earthworm while continuously feeding food waste as the earthworms grow and discharging the fecal soil to the fecal soil collection space in the lower layer; There is known a technique related to earthworm culture and fecal soil collection method using food waste consisting of collecting fecal soil discharged to the fecal soil collection space by a collecting means such as a scraper.

However, the above earthquake farming methods do not suggest the exact growth conditions of the earthworm, and in particular, there is a problem that simply does not provide a earthworm farming method for maximizing the titer of the enzyme having various active functions in the body fluids of the earthworm.

Therefore, the present inventors have completed the present invention after studying the earthworm culture method for maximizing the titer of the enzyme having various active functions in the body fluid of the earthworm, while presenting the accurate earthworm culture conditions.

The present invention provides an earthworm aquaculture method for maximizing the titer of an enzyme having a variety of active functions, especially a thrombolytic property in the earthworm body fluid, as well as an excellent survival rate of the earthworm in the process of farming the earthworm. The purpose is.

In order to achieve the above object, the present invention provides an earthworm aquaculture method for feeding a corroded feed at a temperature of 0.1 ° C to 34 ° C and a humidity of 60% to 80%. It can be achieved by providing an earthworm farming method, characterized in that it is adjusted to 15: 1 to 50: 1 and then fed to feed.

As an earthworm that can be used in the present invention, it is possible to breed using any kind of earthworm, but in particular, a red earthworm (Lumbricus Rubellus), which is widely farmed, is used.

In general, the worm farming method limits the growth conditions of the earthworm to 0.1 ℃ to 34 ℃ and 60% to 80% humidity, which is closely related to the survival rate and rearing status of the earthworm.

In particular, when the temperature of the earthworm rearing is less than 0.1 ℃ problem occurs that the earthworm can be verbal, and when the temperature at the time of rearing exceeds 34 ℃ problem occurs that the heat can occur because the range of the temperature in general I have raised earthworms in me.

In addition, when the humidity of the earthworm rearing is less than 60%, it is difficult to hold the fluid of the earthworm itself, and when it exceeds 80%, there is a problem in that the titer of various enzymes contained in the earthworm body fluid is lowered, and thus the humidity is generally in the range of the above. I have raised earthworms in me.

Under the above conditions, the earthworm feed is corroded and supplied to the food. Conventionally, the earthworm feed has been fed various kinds of waste including food waste, but preferably the earthworm feed is largely carbon-based, nitrogen-based, and inorganic-based. It is desirable to distinguish and use each of them properly in combination for earthworm growth and balanced nutrient intake.

The carbonaceous feed may be a carbonaceous material such as rice bran, sawdust, molasses, etc., and the nitrogen-based feed may be a nitrogen-containing material such as livestock manure, phosphate, activated sludge, meat processing waste or animal residue. In addition, the inorganic system is mainly for the action of the earthworm's digestive action and the dissemination of minerals, rather than the function of food can be used, such as soil, clay, diatomaceous earth.

Particularly, in the present invention, the above-mentioned feeds are properly mixed to adjust the carbon and nitrogen ratio in the feed to 15: 1 to 50: 1 and then corrode, and the corroded food is used as a feed. By controlling, the earthworm survival rate and rearing status are excellent, and the titer of the enzyme having various active functions in the earthworm body fluid, especially the enzyme having thrombolytic properties, can be maximized.

The reason why the carbon and nitrogen ratio in the feed is adjusted to be 15: 1 to 50: 1 is that the higher the carbon content in the feed, that is, when the carbon and nitrogen ratio exceeds 50: 1, the protein content is insufficient in the feed. Therefore, not only is the increase rate and proliferation rate of the earthworm low, especially, the enzyme component that degrades fiber in the thrombolytic enzyme contained in the earthworm body fluid becomes high, and the enzyme component that breaks down the protein decreases. Only smoothly performing the decomposition of the fiber to perform the protein decomposition of the thrombus is not easy to facilitate the action of thrombolysis occurs.

In addition, the lower the carbon content in the feed, that is, when the ratio of carbon and nitrogen is less than 15: 1, the high protein content in the feed increases the proteolytic enzymes in the earthworm vesicles, and thus the enzyme breaks down the earthworm vesicles itself. As a result, the survival rate of the earthworm is not only sharply reduced, but in particular, the enzyme component that degrades protein in the thrombolytic enzyme of the earthworm body fluid increases and the enzyme component that degrades fiber decreases. As a result, the body fluid extracted from the earthworm constitutes a thrombus. Only the protein breakdown is performed smoothly, and the breakdown of the fibers constituting the thrombus is not easy, so the problem of thrombolysis does not occur.

As described above, after controlling the ratio of carbon and nitrogen in the final food, the food is corroded. In this case, the corrosion of the food is less than 90: 1 based on the ratio of carbon and nitrogen so that the corrosion rate is 90% or more. If it is more than that, the corrosion rate should be more than 50%.

The corrosion correlates very much with the survival rate of the earthworms, if the earthworms are eaten without eroding more than 90% of the feed below the carbon-to-nitrogen ratio of 30: 1, they break down the nitrogen in the earthworm vesicles. The acid generated causes protein poisoning, which causes the worm to die.

In addition, when the earthworm is eaten without eroding more than 50% of the feed that is higher than the carbon-to-nitrogen ratio of 30: 1, high heat is generated in the earthworm body due to the activity of high temperature microorganisms, and the earthworm heats up.

Therefore, it is desirable that the corrosion rate is 90% or more when the ratio is less than 30: 1 based on the ratio of carbon and nitrogen to be fed, and the corrosion rate is 50% or more.

The worms that feed on the above-mentioned ratios have excellent survival and rearing status, and they contain fibrinolytic enzymes and proteolytic enzymes in appropriate proportions in the thrombolytic enzymes in the body fluids. The extracted body fluid not only has excellent thrombolytic properties but also maximizes the titer of enzymes having various active functions in other earthworm body fluids.

Hereinafter, the present invention will be described in more detail with reference to the following examples, but the present invention is not limited to the following description.

<Examples 1 to 3>

3000 red earthworms were classified into 5 groups, and sawdust and meat processing waste were mixed at a temperature of 20 ± 5 ℃ and a humidity of 70 ± 10% to adjust the carbon and nitrogen ratio in the feed to the ratio shown in Table 1 below. The aerobic microorganisms were used to corrode the corrosion rates shown in Table 1 below, and the cultured animals were incubated for 6 months while feeding the corroded food to the earthworms.

At this time, the survival rate and the average weight of the farmed earthworms were measured and shown in Table 1. In the above, the average weight is randomly selected by the five earthworms to measure the weight of the average and leave a slight difference.

division Carbon: Nitrogen Corrosion rate (%) Survival rate (%) Average weight Example 1 30 ± 5: 1 95 100 0.40 Example 2 15 ± 5: 1 95 60 0.50 60 30 0.55 Example 3 60 ± 5: 1 95 95 0.2 30 52 0.22

As shown in Table 1, after adjusting the carbon and nitrogen ratio within the scope of the present invention, the corrosion rate is 95% in the case of Example 1, the survival rate is very high to 100% and the average weight of the earthworm is also very high You can see that.

However, in Example 2 in which the carbon-to-nitrogen ratio is less than the range of the present invention, it can be seen that the average weight of the earthworm is very high, and the survival rate varies greatly depending on the degree of corrosion rate, in particular, the corrosion rate is 90% If it is 60% or less, the survival rate can be confirmed that less than 30%.

In addition, in the case of Example 3 carried out so that the ratio of carbon and nitrogen exceeds the range of the present invention, the survival rate is very high when the corrosion rate is 90% or more, and the survival rate is about 50% when it is less than 30%. Compared to the average weight is very low.

<Experimental Example 1 to 2>

Based on the above Table 1, 15g of the crushed liquid obtained by crushing the earthworm and the wild earthworm of Example 1 with excellent survival rate was analyzed according to the colorimetric method of the Korea Health Industry Development Institute and analyzed in Table 2 below. The results are shown.

ingredient Example 1 Curing earthworm moisture 0.27 g 0.245 g Crude protein 6.74 g 5.625g amino acid Histidine 0.26 g 0.20 g Isolinecine 0.31 g 0.22 g leucine 0.54 g 0.45 g Lysine 0.64 g 0.31 g Methionine 0.09g 0.12 g Phenylalanine 0.26 g 0.22 g Throneine 0.33 g 0.22 g TryptopHan 0.05g - Valine 0.38 g 0.27 g Alanine 0.44 g 0.34 g Arginine 0.45 g 0.34 g Aspartic acid 0.73 g 0.23 g Cystine 0.05g 0.18 g Glutamic acid 1.04 g 0.36 g Glycine 0.33 g 0.11 g Proline 0.44 g 0.13 g Serine 0.32 g 0.22 g Tyrosine 0.19 g 0.22 g Etc 1.14 g 4.99g

As shown in Table 1, it can be confirmed through Table 2 that the earthworms cultured by the earthworm farming method of the present invention have a very high content of the active ingredient compared to wild earthworms.

As described above, the present invention is an earthworm for maximizing the titer of an enzyme having a variety of active functions in the earthworm body fluid, especially an thrombolytic property, in addition to excellent survival rate of the earthworm in the process of farming the earthworm. It is a useful invention to provide aquaculture methods.

Claims (3)

In the earthworm culture method for feeding the corroded feed at a temperature of 0.1 ℃ to 34 ℃, humidity 60% to 80%, the carbon and nitrogen ratio in the feed with the feed is adjusted to 15: 1 to 50: 1 Next, the earthworm culture method characterized in that it is supplied to feed by corrosion. The method of claim 1, wherein the corrosion is based on a ratio of carbon to nitrogen of 30: 1, when the corrosion rate is less than 90%, and when the corrosion rate is higher than that, the corrosion rate is 50% or more. Earthworm farming method. 3. The feed according to claim 1 or 2, wherein the feed is a carbon-based carbonaceous material such as rice bran, sawdust, molasses, etc., and nitrogen-based and food-rich nitrogen, such as livestock manure, phosphorus, activated sludge, meat processing waste, animal residues, and the like. Earthworm farming method, characterized in that the composition of the inorganic earth, such as soil, clay, diatomaceous earth, etc., which is suitable for the action of helping the digestive activity of the earthworm and the diffusion of minerals.