WO2011108810A2 - Amino acid liquid fertilizer using marine waste, and preparation method thereof - Google Patents

Amino acid liquid fertilizer using marine waste, and preparation method thereof Download PDF

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Publication number
WO2011108810A2
WO2011108810A2 PCT/KR2011/000403 KR2011000403W WO2011108810A2 WO 2011108810 A2 WO2011108810 A2 WO 2011108810A2 KR 2011000403 W KR2011000403 W KR 2011000403W WO 2011108810 A2 WO2011108810 A2 WO 2011108810A2
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Prior art keywords
amino acid
waste
acid liquid
pulverized
weight
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PCT/KR2011/000403
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French (fr)
Korean (ko)
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WO2011108810A3 (en
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박해성
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Park Hae-Sung
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Publication of WO2011108810A2 publication Critical patent/WO2011108810A2/en
Publication of WO2011108810A3 publication Critical patent/WO2011108810A3/en

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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/10—Fertilisers containing plant vitamins or hormones
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00—Fertilisers made from animal corpses, or parts thereof
    • C05F1/002—Fertilisers made from animal corpses, or parts thereof from fish or from fish-wastes
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C05D9/02—Other inorganic fertilisers containing trace elements
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

Definitions

  • the present invention relates to an amino acid liquid fertilizer using a variety of fishery waste and a method for producing the same, more specifically, a variety of fishery waste such as fish processing by-products, farmed waste fish, fish waste (head, bone, fins, internal organs, etc.) as a raw material
  • a variety of fishery waste such as fish processing by-products, farmed waste fish, fish waste (head, bone, fins, internal organs, etc.
  • fishery waste such as fish processing by-products, farmed waste fish, fish waste (head, bone, fins, internal organs, etc.
  • the present invention relates to a high-quality amino acid liquor having excellent preservability, which requires a short time, and also saves cost, and a method of manufacturing the same.
  • Landfill treatment one of the treatment methods for fishery waste, acts as a cause of environmental pollution in the surrounding area as well as groundwater contamination, and there are many incidental problems. Therefore, in order to solve these problems as well as to efficiently utilize fishery waste, a method of manufacturing fertilizer using the same has attracted attention as an alternative.
  • an object of the present invention is to provide a high quality amino acid liquor production method that can be processed in a short time and a simple process in a short time by a biological method without generating odor to solve the problems of the prior art.
  • the present invention is a freezing step of freezing the seafood waste in a container, a crushing and grinding step of crushing and then crushing the frozen seafood waste passed through the freezing step to obtain a pulverized product in the paste state,
  • the proteolytic enzyme and water is added, and then liquefied by hot water at 35 ⁇ 60 °C hot water and first stirred for 15 to 20 hours at 100 ⁇ 120rpm Liquefaction step of obtaining the pulverized liquid, the liquefaction step through the liquefaction step of 40 ⁇ 80rpm for 10-15 hours by agitation, fermentation step of fermentation step, fermentation step, filtration to obtain the filtrate by filtering the pulverized liquid after the fermentation step
  • the filtrate after the step and the filtration step is transferred to a tank equipped with a stirrer and then nitrogen (N), phosphorus (P), kali (K), boron (B), zinc (Zn), iron (N), nitrogen (N), phosphorus (P), kali (K), boron (B), zinc (
  • the protease is preferably subtilisin.
  • the liquefaction step may be made by adding 5 to 15 parts by weight of water and 0.5 to 3 parts by weight of protease based on 100 parts by weight of fishery waste.
  • the filtration step may be performed by filtration through a screen network of 5 ⁇ 10 mesh (mesh).
  • the dissolving step may be made by dissolving 2-6 parts by weight of nitrogen, 0.5-3 parts by weight of phosphorus and 2-6 parts by weight of Kali.
  • the present invention provides an environmentally friendly amino acid liquid ratio using a fishery waste, characterized in that the manufacturing method as described above.
  • the manufacturing method of the present invention solves the problem of disposal of marine waste, which is a serious environmental problem by using various marine waste such as fish waste, fish processing by-products, aquaculture waste fish, etc.
  • various marine waste such as fish waste, fish processing by-products, aquaculture waste fish, etc.
  • the manufacturing cost of liquid fertilizer can be saved as well.
  • subtilisin as a protease, the pretreatment alkalizing process is not necessary. It can manufacture.
  • the amino acid liquid fertilizer prepared by the method of the present invention does not need to fertilize separate calcium and phosphorus components because calcium and phosphorus contained in fish bones are eluted and present as water-soluble calcium and phosphorus in the liquid fertilizer. It is a high concentration of 15-18%, which is diluted 1000 ⁇ 1500 times for foliar fertilization and defoliation of leafy vegetables and fruits, and is excellent in increasing taste and aroma of plants, improving color, increasing yield, preventing high temperature and cold, and reducing pests. Has an effect.
  • the present invention relates to a method for producing environmentally friendly amino acid liquid fertilizer using a marine waste (hereinafter referred to as the 'manufacturing method of the present invention') and the amino acid liquid fertilizer prepared thereby
  • the manufacturing method of the present invention is to freeze the marine waste in a container Crushing and grinding step of crushing and then crushing frozen fishery waste that passed through the freezing step and grinding to obtain a paste-like pulverized product, and putting the paste-like crushed product that passed through the crushing and crushing step into a fermenter and proteolytic enzyme
  • liquefied step of obtaining a liquefied pulverized liquid by agitation with hot water of 35 ⁇ 60 °C and primary stirring for 15 to 20 hours at 100 ⁇ 120rpm, 40 ⁇ 80rpm Fermentation step of aging and fermentation by secondary stirring for 10 to 15 hours
  • filtration step to obtain a filtrate by filtering the grinding liquid passed through the fermentation step and the filtration step Is transferred to a tank equipped with a stirrer
  • Seafood wastes include fish waste, fish processing by-products, farmed waste fish, etc. These fish wastes are preferably frozen in containers or boxes such as plastic bags in order to reduce odor and prevent spoilage.
  • the freezing temperature may be any temperature as long as it can reduce odor of fishery waste and prevent corruption, and preferably -20 to -10 ° C. If the temperature is -10 ° C or higher, the refrigeration may not be sufficiently performed, and thus the effect of reducing odor and anti-corruption may be reduced.
  • the crushing and pulverizing step of the present invention is a step of crushing the frozen seafood waste through the freezing step with a crusher and then pulverizing with a pulverizer to obtain a pulverized product in the form of a paste such as thick porridge. It can be expanded to facilitate the liquefaction of fishery waste and further maximize the specific surface area to improve the reaction with protease, degradation efficiency and shorten the reaction time.
  • the liquefaction step of the present invention is a step of obtaining a liquefied pulverized liquid by putting the pulverized product in the past in the crushing and grinding step into a fermentation tank, adding proteolytic enzymes and water, followed by boiling water and primary stirring. It is recommended to add 5 to 15 parts by weight of water and 0.5 to 3 parts by weight of protease based on 100 parts by weight of fishery waste.
  • protease pepsin, trypsin, chymotrypsin, actinidine, actinine, ficin, pancreatin, rennin, bromline ), Papain, subtilisin, etc. may be any one having a proteolytic function, but preferably subtilisin is preferable.
  • Subtilisin is an alkaline serin protease produced by various Bacillus species and other microorganisms. It is particularly preferred as a high protein degrading enzyme that can facilitate the manufacturing process, simplify and reduce the manufacturing cost, and has the effect of preventing the decay of the finished product.
  • Usable subtilisins are any such as subtilisin BPN isolated from Bacillus amyloliquefaciens, subtilisin Carlsberg isolated from Bacillus licheniformis obviously.
  • the protein contained in the fishery waste is finely broken, and the plant can be converted to more than 99% of the 20 kinds of amino acids that can be used as a long-term nutrient.
  • the filtration step of the present invention is a step of obtaining a filtrate by filtering the milled liquid passed through the fermentation step, it is preferable to filter with a screen network of 5 ⁇ 10 mesh (mesh).
  • the filtrate after the filtration step is transferred to a tank equipped with a stirrer, and then nitrogen (N), phosphorus (P), kali (K), boron (B), zinc (Zn), and iron (Fe). ), Goto (Mg), molybdenum (Mo), manganese (Mn) is added to any one or more of the step of melting.
  • Nitrogen is a member of chlorophyll, which is involved in photosynthesis, promotes the growth of crops, and aids crop uptake by kali, goto, and calcium.
  • the yield is increased by sufficiently supplying carbohydrates necessary for fruit hypertrophy by the leaf area.
  • Phosphorus improves eggplant and leaf growth, increases yield and fruit tree, increases sweetness, and decreases sourness to improve fruit quality. It also promotes root development.
  • Kali inhibits loss of water by transpiration and is required for root elongation and fruit hypertrophy.
  • boron reduces the effects of lime deficiency and zinc prevents sulfidation and leaf miniaturization.
  • Iron is involved in chlorophyll formation, and goto is a component of chlorophyll in plants, which, when depleted, does not form chlorophyll, resulting in decreased photosynthesis. Molybdenum causes leaf twisting when deficient.
  • the material added to the dissolving step is preferably 2 to 6 parts by weight of nitrogen, 0.5 to 3 parts by weight of phosphorus and 2 to 6 parts by weight of kali.
  • Amino acid liquid ratio of the present invention can be prepared by the production method of the present invention as described above.
  • the brownish brown liquid was filtered through a screen of 7 mesh to obtain a filtrate, and 4 parts by weight of nitrogen, 2 parts by weight of phosphorus, and 4 parts by weight of Kali were added and dissolved to prepare an amino acid solution.
  • Amino acid liquor thus prepared did not cause odor. In addition, even after four weeks, corruption did not proceed, confirming the excellent retention.
  • the manufacturing method of the present invention solves the problem of disposal of marine waste, which is a serious environmental problem by using various marine waste such as fish waste, fish processing by-products, aquaculture waste fish, etc.
  • various marine waste such as fish waste, fish processing by-products, aquaculture waste fish, etc.
  • the manufacturing cost of liquid fertilizer can be saved as well.
  • subtilisin as a protease, the pretreatment alkalizing process is not necessary. It can manufacture.
  • the amino acid liquid fertilizer prepared by the method of the present invention does not have to fertilize separate calcium and phosphorus components because calcium and phosphorus contained in fish bones are eluted and present as water-soluble calcium and phosphorus in the liquid fertilizer. It is a high concentration of 15-18%, which is diluted 1000 ⁇ 1500 times for foliar fertilization and defoliation of leafy vegetables and fruits, and it is excellent in plant taste and aroma, color enhancement, yield increase, prevention of high temperature and cold, and reduction of pests. Has an effect.

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Environmental & Geological Engineering (AREA)
  • Inorganic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to an amino acid liquid fertilizer using various marine waste, and a preparation method thereof, and more specifically, to a preparation method of an environmentally friendly amino acid liquid fertilizer using marine waste comprising: the freezing step of putting marine waste in a container to freeze the marine waste; the crushing and pulverizing step of crushing the frozen marine waste went through the freezing step and pulverized the crushed frozen marine to obtain the paste state of the pulverized product; the liquefaction step of injecting the paste state of the pulverized product went through the crushing and pulverizing step into a fermentation tank, adding a protease and water, heating the same in a heated bath at 35-60 ℃, and first stirring the obtained solution at 100-120 rpm for 15-20 hours to obtain a liquefied pulverized solution; the fermentation step of second stirring the pulverized solution went through the liquefaction step at 40-80 rpm for 10-15 hours to mature and ferment the same; the filtration step of filtering the pulverized solution went through the fermentation step to obtain a filtrate; and the dissolving step of transferring the filtrate went through the filtration step to a tank attached with a stirrer, and adding one or more substances of nitrogen (N), phosphorus (P), potassium (K), boron (B), zinc (Zn), iron (Fe), magnesia (Mg), molybdenum (Mo), manganese (Mn) to dissolve the same, and an amino acid liquid fertilizer prepared thereby. According to the present invention, it is possible to treat marine waste, which is cheap material, by a simple process within a short period of time through a biological method without generation of odor and to improve conservation stability of a prepared amino acid liquid fertilizer.

Description

수산 폐기물을 이용한 아미노산 액비 및 그 제조방법Amino acid fertilizer using fishery waste and its manufacturing method
본 발명은 각종 수산 폐기물을 이용한 아미노산 액비 및 그 제조방법에 관한 것으로서, 더욱 상세하게는 수산가공 부산물, 양식폐사어류, 생선폐기물(머리, 뼈, 지느러미, 내장 등) 등 각종의 수산 폐기물을 원료로 함으로써 수산 폐기물의 처리 문제를 해결함은 물론, 악취 발생의 문제 없이 값싼 원료를 사용하여 액비의 제조비용 또한 절약할 수 있으며 단백질 분해효소로서 서브틸리신을 사용함으로써 전처리 알칼리화 공정이 필요치 않아 제조 공정이 간단하고 단시간이 소요되며 비용 또한 절약되는 보존성이 뛰어난 양질의 아미노산 액비 및 그 제조방법에 관한 것이다.The present invention relates to an amino acid liquid fertilizer using a variety of fishery waste and a method for producing the same, more specifically, a variety of fishery waste such as fish processing by-products, farmed waste fish, fish waste (head, bone, fins, internal organs, etc.) as a raw material This solution not only solves the problem of aquatic waste, but also saves the manufacturing cost of liquid fertilizer by using cheap raw materials without the problem of odor generation. By using the subtilisin as protease, the pretreatment alkalizing process is not necessary. The present invention relates to a high-quality amino acid liquor having excellent preservability, which requires a short time, and also saves cost, and a method of manufacturing the same.
일반 수산시장 또는 생선가공공장, 대단위 수산물 유통단지 등에서 쏟아지는 생선 폐기물, 수산가공 부산물 등과 같은 각종 수산 폐기물의 양이 급증하고 있다. 이와 같이 배출되는 수산 폐기물이 부패될 때는 악취가 심하고 해충이 발생하며 부패수는 토양과 수질을 오염시키는 등의 환경오염을 유발하며 나아가 생태계를 위협하는 요인이 되고 있다. 따라서 이의 처리문제가 심각한 사회문제로 대두되고 있다.The amount of various fish wastes, such as fish wastes and fish processing by-products, which are poured from general fish markets, fish processing factories, and large-scale seafood distribution complexes, are increasing rapidly. When the discharged marine wastes are decayed, bad smells and pests are generated, and decayed water causes environmental pollution such as polluting soil and water quality, and also threatens the ecosystem. Therefore, its disposal problem is a serious social problem.
수산 폐기물의 처리방법 중 하나인 매립 처리는 지하수 오염은 물론 주변 지역의 환경오염의 원인으로 작용하며 많은 부수적인 문제가 발생하고 있다. 따라서 이러한 문제를 해결함은 물론 수산 폐기물을 효율적으로 활용하기 위하여 이를 이용하여 비료를 제조하는 방법이 대안으로써 주목받고 있다. Landfill treatment, one of the treatment methods for fishery waste, acts as a cause of environmental pollution in the surrounding area as well as groundwater contamination, and there are many incidental problems. Therefore, in order to solve these problems as well as to efficiently utilize fishery waste, a method of manufacturing fertilizer using the same has attracted attention as an alternative.
몇몇 지방자치단체에서는 고속발효기를 설치하여 음식물쓰레기와 생선 폐기물을 원료로 한 비료를 얻기 위한 처리를 하고 있는데 비료를 얻기까지 발효가 완숙되는 기간이 약 4개월 정도의 장시간이 소요되어 현실적으로 생선 폐기물의 처리를 감당하지 못하고 있는 실정이며 이로 인한 비료 제조비용 상승 문제 등 사실상 실용화되기에는 여러 가지 문제점이 있다. Some local governments have installed high-speed fermenters to process food waste and fertilizers from fish wastes.The fermentation process takes about four months to produce fertilizers. The situation is not able to handle the treatment and there are a number of problems that are practical to be practical, such as a problem of rising fertilizer manufacturing costs.
또한 비료를 얻기까지 장시간이 소요되어 생선비린내 및 단백질의 부패취가 극심하여 생선 폐기물 주변에 파리, 모기 등의 해충 등이 많이 발생하므로 위생적인 면에서도 상당히 나쁜 영향을 끼치고 있다.In addition, it takes a long time to obtain fertilizers, and fish odors and protein odors are so severe that many insects such as flies and mosquitoes are generated around the fish waste, which has a bad effect on hygiene.
따라서 본 발명의 목적은 상기 종래기술의 문제점을 해결하기 위하여 수산 폐기물을 악취 발생 없이 생물학적 방법으로 단시간 내 간단한 공정과 저렴한 비용으로 처리할 수 있는 양질의 아미노산 액비 제조방법을 제공하는 것이다.Therefore, an object of the present invention is to provide a high quality amino acid liquor production method that can be processed in a short time and a simple process in a short time by a biological method without generating odor to solve the problems of the prior art.
또한 본 발명의 목적은 상기와 같은 제조방법을 통하여 제조된 보존성이 뛰어난 양질의 아미노산 액비를 제공하는 것이다.It is also an object of the present invention to provide a good amino acid liquid ratio of excellent preservability prepared through the above production method.
상기와 같은 목적을 달성하기 위하여, 본 발명은 수산 폐기물을 용기에 담아 냉동시키는 냉동단계, 상기 냉동단계를 거친 냉동된 수산 폐기물을 파쇄한 후 분쇄하여 페이스트 상태의 분쇄물을 얻는 파쇄·분쇄단계, 상기 파쇄·분쇄단계를 거친 페이스트 상태의 분쇄물을 발효조에 투입하고 단백질 분해효소 및 물을 첨가한 후, 35~60℃의 온수로 중탕하고 100~120rpm으로 15~20시간 동안 1차 교반하여 액화된 분쇄액을 얻는 액화단계, 상기 액화단계를 거친 분쇄액을 40~80rpm으로 10~15시간 동안 2차 교반하여 숙성, 발효시키는 발효단계, 상기 발효단계를 거친 분쇄액을 여과하여 여액을 얻는 여과단계 및 상기 여과단계를 거친 여액을 교반기가 부착된 탱크로 이송한 후 질소(N), 인(P), 칼리(K), 붕소(B), 아연(Zn), 철(Fe), 고토(Mg), 몰리브덴(Mo), 망간(Mn) 중 어느 하나 이상을 첨가하여 용해시키는 용해단계를 포함하는 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비의 제조방법을 제공한다.In order to achieve the above object, the present invention is a freezing step of freezing the seafood waste in a container, a crushing and grinding step of crushing and then crushing the frozen seafood waste passed through the freezing step to obtain a pulverized product in the paste state, After the crushed and pulverized step of the pulverized product is added to the fermenter, the proteolytic enzyme and water is added, and then liquefied by hot water at 35 ~ 60 ℃ hot water and first stirred for 15 to 20 hours at 100 ~ 120rpm Liquefaction step of obtaining the pulverized liquid, the liquefaction step through the liquefaction step of 40 ~ 80rpm for 10-15 hours by agitation, fermentation step of fermentation step, fermentation step, filtration to obtain the filtrate by filtering the pulverized liquid after the fermentation step The filtrate after the step and the filtration step is transferred to a tank equipped with a stirrer and then nitrogen (N), phosphorus (P), kali (K), boron (B), zinc (Zn), iron (Fe), goto ( Mg), molybdenum (Mo) or manganese (Mn) It provides a method for producing environmentally friendly amino acid liquid fertilizer using a fishery waste, characterized in that it comprises a dissolution step of dissolving by adding a phase.
상기 단백질 분해효소는 서브틸리신이 바람직하다.The protease is preferably subtilisin.
상기 액화단계는, 수산 폐기물 100 중량부를 기준으로 물을 5~15 중량부, 단백질 분해효소를 0.5~3 중량부로 첨가하여 이루어질 수 있다.The liquefaction step may be made by adding 5 to 15 parts by weight of water and 0.5 to 3 parts by weight of protease based on 100 parts by weight of fishery waste.
상기 여과단계는, 5~10메쉬(mesh)의 스크린 망으로 여과하여 이루어질 수 있다.The filtration step may be performed by filtration through a screen network of 5 ~ 10 mesh (mesh).
상기 용해단계는, 질소 2~6 중량부, 인 0.5~3 중량부 및 칼리 2~6 중량부를 첨가한 후 용해하여 이루어질 수 있다.The dissolving step may be made by dissolving 2-6 parts by weight of nitrogen, 0.5-3 parts by weight of phosphorus and 2-6 parts by weight of Kali.
또한 본 발명은 상기와 같은 제조방법으로 제조되는 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비를 제공한다.In another aspect, the present invention provides an environmentally friendly amino acid liquid ratio using a fishery waste, characterized in that the manufacturing method as described above.
본 발명의 제조방법은 생선폐기물, 수산가공 부산물, 양식폐사어류 등 각종의 수산 폐기물을 원료로 함으로써 환경적으로 심각한 문제가 되고 있는 수산 폐기물의 처리 문제를 해결함은 물론, 악취 발생의 문제 없이 값싼 원료를 사용하여 액비의 제조비용 또한 절약할 수 있으며 단백질 분해효소로서 서브틸리신을 사용함으로써 전처리 알칼리화 공정이 필요치 않게 되어 제조 공정이 간단하고 단시간이 소요되며 비용 또한 절약하면서 보존성이 뛰어난 양질의 아미노산 액비를 제조할 수 있다.The manufacturing method of the present invention solves the problem of disposal of marine waste, which is a serious environmental problem by using various marine waste such as fish waste, fish processing by-products, aquaculture waste fish, etc. By using raw materials, the manufacturing cost of liquid fertilizer can be saved as well. By using subtilisin as a protease, the pretreatment alkalizing process is not necessary. It can manufacture.
또한 본 발명의 방법에 의해 제조한 아미노산 액비는 생선뼈 등에 함유된 칼슘 및 인이 용출되어 액비 중에 수용성 칼슘 및 인으로 존재하므로 별도의 칼슘 및 인 성분을 시비하지 않아도 되며, 완제품의 아미노산 액비의 농도는 15～18％의 고농도로써 엽채류, 과채류의 엽면시비 및 간주용으로 1000～1500배 희석한 희석액으로도 식물의 맛과 향 증가, 색도 향상, 생산량 증가, 고온 및 냉해 예방, 병충해 감소 등의 탁월한 효과를 지닌다.In addition, the amino acid liquid fertilizer prepared by the method of the present invention does not need to fertilize separate calcium and phosphorus components because calcium and phosphorus contained in fish bones are eluted and present as water-soluble calcium and phosphorus in the liquid fertilizer. It is a high concentration of 15-18%, which is diluted 1000 ～ 1500 times for foliar fertilization and defoliation of leafy vegetables and fruits, and is excellent in increasing taste and aroma of plants, improving color, increasing yield, preventing high temperature and cold, and reducing pests. Has an effect.
본 발명은 수산 폐기물을 이용한 친환경 아미노산 액비의 제조방법(이하 '본 발명의 제조방법'이라 함) 및 이에 의하여 제조된 아미노산 액비에 관한 것으로서, 본 발명의 제조방법은 수산 폐기물을 용기에 담아 냉동시키는 냉동단계, 상기 냉동단계를 거친 냉동된 수산 폐기물을 파쇄한 후 분쇄하여 페이스트 상태의 분쇄물을 얻는 파쇄·분쇄단계, 상기 파쇄·분쇄단계를 거친 페이스트 상태의 분쇄물을 발효조에 투입하고 단백질 분해효소 및 물을 첨가한 후, 35~60℃의 온수로 중탕하고 100~120rpm으로 15~20시간 동안 1차 교반하여 액화된 분쇄액을 얻는 액화단계, 상기 액화단계를 거친 분쇄액을 40~80rpm으로 10~15시간 동안 2차 교반하여 숙성, 발효시키는 발효단계, 상기 발효단계를 거친 분쇄액을 여과하여 여액을 얻는 여과단계 및 상기 여과단계를 거친 여액을 교반기가 부착된 탱크로 이송한 후 질소(N), 인(P), 칼리(K), 붕소(B), 아연(Zn), 철(Fe), 고토(Mg), 몰리브덴(Mo), 망간(Mn) 중 어느 하나 이상을 첨가하여 용해시키는 용해단계를 포함하는 것을 특징으로 한다.The present invention relates to a method for producing environmentally friendly amino acid liquid fertilizer using a marine waste (hereinafter referred to as the 'manufacturing method of the present invention') and the amino acid liquid fertilizer prepared thereby, the manufacturing method of the present invention is to freeze the marine waste in a container Crushing and grinding step of crushing and then crushing frozen fishery waste that passed through the freezing step and grinding to obtain a paste-like pulverized product, and putting the paste-like crushed product that passed through the crushing and crushing step into a fermenter and proteolytic enzyme And after the addition of water, liquefied step of obtaining a liquefied pulverized liquid by agitation with hot water of 35 ~ 60 ℃ and primary stirring for 15 to 20 hours at 100 ~ 120rpm, 40 ~ 80rpm Fermentation step of aging and fermentation by secondary stirring for 10 to 15 hours, filtration step to obtain a filtrate by filtering the grinding liquid passed through the fermentation step and the filtration step Is transferred to a tank equipped with a stirrer and then nitrogen (N), phosphorus (P), kali (K), boron (B), zinc (Zn), iron (Fe), goto (Mg), molybdenum (Mo), It characterized in that it comprises a dissolution step of dissolving by adding any one or more of manganese (Mn).
수산 폐기물은 생선폐기물, 수산가공 부산물, 양식폐사어류 등을 포함하며 이러한 수산 폐기물은 악취를 감소시키고 부패를 방지하기 위해 상자 또는 비닐팩 등의 용기에 담아 냉동시키는 것이 바람직하다.Seafood wastes include fish waste, fish processing by-products, farmed waste fish, etc. These fish wastes are preferably frozen in containers or boxes such as plastic bags in order to reduce odor and prevent spoilage.
본 발명의 냉동단계에 있어서 냉동 온도는 수산 폐기물의 악취를 감소시키고 부패를 방지할 수 있는 온도라면 어떠한 온도라도 무방하며, 바람직하게는 -20~-10℃가 좋다. -10℃ 이상이면 냉동이 충분히 이루어지지 않아 악취 감소 및 부패 방지의 효과가 떨어질 수 있으며, -20℃ 이하이면 효과의 차이는 크지 않지만 비용이 증가하는 문제가 있다.In the freezing step of the present invention, the freezing temperature may be any temperature as long as it can reduce odor of fishery waste and prevent corruption, and preferably -20 to -10 ° C. If the temperature is -10 ° C or higher, the refrigeration may not be sufficiently performed, and thus the effect of reducing odor and anti-corruption may be reduced.
본 발명의 파쇄·분쇄단계는 상기 냉동단계를 거쳐 냉동된 수산 폐기물을 파쇄기로 파쇄한 후 분쇄기로 분쇄하여 걸쭉한 죽과 같은 페이스트 상태의 분쇄물을 얻는 단계로서 이와 같은 공정을 통해 열과 접촉하는 면적이 넓어져 수산 폐기물의 액화를 용이하게 하며 나아가 비표면적을 극대화하여 단백질 분해효소와의 반응 및 분해 효율을 향상시키고 반응시간을 단축할 수 있다.The crushing and pulverizing step of the present invention is a step of crushing the frozen seafood waste through the freezing step with a crusher and then pulverizing with a pulverizer to obtain a pulverized product in the form of a paste such as thick porridge. It can be expanded to facilitate the liquefaction of fishery waste and further maximize the specific surface area to improve the reaction with protease, degradation efficiency and shorten the reaction time.
본 발명의 액화단계는 상기 파쇄·분쇄단계를 거친 페이스트 상태의 분쇄물을 발효조에 투입하고 단백질 분해효소와 물을 첨가한 후, 중탕 및 1차 교반하여 액화된 분쇄액을 얻는 단계로서, 바람직하게는 수산 폐기물 100 중량부를 기준으로 물을 5~15 중량부, 단백질 분해효소를 0.5~3 중량부로 첨가하는 것이 좋다.The liquefaction step of the present invention is a step of obtaining a liquefied pulverized liquid by putting the pulverized product in the past in the crushing and grinding step into a fermentation tank, adding proteolytic enzymes and water, followed by boiling water and primary stirring. It is recommended to add 5 to 15 parts by weight of water and 0.5 to 3 parts by weight of protease based on 100 parts by weight of fishery waste.
상기 단백질 분해효소로는 펩신(pepsin), 트립신(trypsin), 키모트립신(chymotrypsin), 액티니딘(actinidine), 피신(ficin), 판크레아틴(pancreatin), 레닌(rennin), 브로멜라인(bromelin), 파파인(papain), 서브틸리신(subtilisin) 등 단백질 분해 기능을 가진 것이면 어떤 것이든 무방하나 바람직하게는 서브틸리신이 좋다.As the protease, pepsin, trypsin, chymotrypsin, actinidine, actinine, ficin, pancreatin, rennin, bromline ), Papain, subtilisin, etc. may be any one having a proteolytic function, but preferably subtilisin is preferable.
트립신, 키모트립신 등의 단백질 분해효소를 사용하는 경우, 전처리로서 수산화나트륨(NaOH), 수산화칼륨(KOH) 등과 같은 강알칼리를 첨가하여 유기물의 단백질을 가수분해하는 강알칼리화 공정이 요구되어 공정이 복잡해지고 그에 따라 제조비용이 증가하는 문제가 있다. When using proteolytic enzymes such as trypsin and chymotrypsin, a strong alkaline process for hydrolyzing proteins of organic matter by adding strong alkalis such as sodium hydroxide (NaOH) and potassium hydroxide (KOH) as a pretreatment is complicated. Accordingly, there is a problem that the manufacturing cost increases.
반면 서브틸리신은, 다양한 바실러스(Bacillus) 종 및 기타의 미생물이 생산하는 알칼리성 세린프로테아제(alkali serin protease)로서, 강알칼리화 전처리 공정이 필요치 않고 pH 5~8과 같은 광범위한 pH 영역조건에서 아미노산 액비 제조가 가능하므로 제조공정의 용이, 단순화 및 제조비용 절약이 가능하며 완제품의 부패를 방지하는 효과가 있는 고단백 분해효소로서 특히 바람직하다. 사용 가능한 서브틸리신은 바실러스 아밀로리쿼파시엔스(Bacillus amyloliquefaciens)로부터 분리된 서브틸리신 BPN(subtilisin BPN), 바실러스 리케니포미스(Bacillus licheniformis)로부터 분리된 서브틸리신 칼스버그(subtilisin Carlsberg) 등 어떠한 것이든 무방하다. Subtilisin, on the other hand, is an alkaline serin protease produced by various Bacillus species and other microorganisms. It is particularly preferred as a high protein degrading enzyme that can facilitate the manufacturing process, simplify and reduce the manufacturing cost, and has the effect of preventing the decay of the finished product. Usable subtilisins are any such as subtilisin BPN isolated from Bacillus amyloliquefaciens, subtilisin Carlsberg isolated from Bacillus licheniformis Anyway.
이와 같은 단백질 분해효소의 첨가에 의해 수산 폐기물에 함유된 단백질이 잘게 분해되어 식물이 장기간 영양분으로 사용가능한 20여 종의 아미노산으로 99% 이상 전환될 수 있다.By the addition of such protease, the protein contained in the fishery waste is finely broken, and the plant can be converted to more than 99% of the 20 kinds of amino acids that can be used as a long-term nutrient.
본 발명의 여과단계는 상기 발효단계를 거친 분쇄액을 여과하여 여액을 얻는 단계로서 바람직하게는 5~10메쉬(mesh)의 스크린 망으로 여과하는 것이 좋다.The filtration step of the present invention is a step of obtaining a filtrate by filtering the milled liquid passed through the fermentation step, it is preferable to filter with a screen network of 5 ~ 10 mesh (mesh).
본 발명의 용해단계는 상기 여과단계를 거친 여액을 교반기가 부착된 탱크로 이송한 후 질소(N), 인(P), 칼리(K), 붕소(B), 아연(Zn), 철(Fe), 고토(Mg), 몰리브덴(Mo), 망간(Mn) 중 어느 하나 이상을 첨가한 후 용해하는 공정으로 이루어진다. In the dissolution step of the present invention, the filtrate after the filtration step is transferred to a tank equipped with a stirrer, and then nitrogen (N), phosphorus (P), kali (K), boron (B), zinc (Zn), and iron (Fe). ), Goto (Mg), molybdenum (Mo), manganese (Mn) is added to any one or more of the step of melting.
질소는 광합성에 관계하는 엽록소의 구성원소로서 작물의 생육을 촉진하며 칼리, 고토, 칼슘의 작물 흡수를 돕는다. 또한 엽면적을 확대시키고 초기의 전엽수를 증가시켜 엽면적의 확대에 의한 과실비대에 필요한 탄수화물을 충분히 공급함으로써 수량을 증가시킨다. Nitrogen is a member of chlorophyll, which is involved in photosynthesis, promotes the growth of crops, and aids crop uptake by kali, goto, and calcium. In addition, by increasing the leaf area and increasing the total number of early leaves, the yield is increased by sufficiently supplying carbohydrates necessary for fruit hypertrophy by the leaf area.
인은 가지와 잎의 생장을 충실하게 하고 수량 및 착과수를 증가시키며 단맛을 증가시키는 반면 신맛을 감소시켜 과실의 품질을 좋게 한다. 또한 뿌리의 발육을 촉진한다.Phosphorus improves eggplant and leaf growth, increases yield and fruit tree, increases sweetness, and decreases sourness to improve fruit quality. It also promotes root development.
칼리는 증산에 의한 수분 상실을 억제하며 뿌리 신장, 과실 비대에 필요하다. 그밖에, 붕소는 석회결핍의 영향을 줄여주고, 아연은 황화 및 잎의 소형화를 방지한다. 철은 엽록소 형성에 관계하고, 고토는 식물의 엽록소의 구성요소로서 결핍시는 엽록소가 형성되지 않아 광합성 작용이 저하된다. 몰리브덴은 결핍시 잎이 꼬이는 증상이 나타난다.Kali inhibits loss of water by transpiration and is required for root elongation and fruit hypertrophy. In addition, boron reduces the effects of lime deficiency and zinc prevents sulfidation and leaf miniaturization. Iron is involved in chlorophyll formation, and goto is a component of chlorophyll in plants, which, when depleted, does not form chlorophyll, resulting in decreased photosynthesis. Molybdenum causes leaf twisting when deficient.
상기 용해단계에 첨가되는 물질은 바람직하게는 질소 2~6 중량부, 인 0.5~3 중량부 및 칼리 2~6 중량부가 좋다.The material added to the dissolving step is preferably 2 to 6 parts by weight of nitrogen, 0.5 to 3 parts by weight of phosphorus and 2 to 6 parts by weight of kali.
본 발명의 아미노산 액비는 상기와 같은 본 발명의 제조방법에 의하여 제조될 수 있다.Amino acid liquid ratio of the present invention can be prepared by the production method of the present invention as described above.
이하 본 발명을 하기 실시예를 통해 더욱 상세히 설명하면 다음과 같다. 다만 하기의 실시예는 본 발명에 대한 이해를 돕기 위한 것일 뿐, 본 발명이 이에 한정되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to the following examples. However, the following examples are merely to help the understanding of the present invention, the present invention is not limited thereto.
[실시예1]Example 1
생선 폐기물 100 중량부를 용기에 담아 -15℃로 냉동시킨 후 이를 파쇄기에 넣어 파쇄한 후 다시 분쇄기로 분쇄하여 걸쭉한 죽과 같은 페이스트 상태의 분쇄물을 얻었다. 이를 발효조에 투입하고 서브틸리신 2 중량부 및 물 10 중량부를 첨가하였다. 이를 50℃의 온수로 중탕하고 110rpm으로 15시간 동안 1차 교반하여 액화된 분쇄액을 얻은 다음, 분쇄액을 60rpm으로 13시간 동안 2차 교반하여 숙성, 발효시켰다. 숙성, 발효를 거친 흑갈색의 분쇄액을 7메쉬의 스크린 망으로 여과하여 여액을 얻은 후 질소 4 중량부, 인 2 중량부, 및 칼리 4 중량부를 첨가하고 용해시켜 아미노산 액비를 제조하였다. 100 parts by weight of fish waste was put in a container, frozen at -15 ° C, and then crushed into a crusher, and then crushed by a grinder to obtain a paste-like pulverized powder. It was added to the fermenter and 2 parts by weight of subtilisin and 10 parts by weight of water were added. The mixture was bathed in warm water at 50 ° C. and stirred first at 110 rpm for 15 hours to obtain a liquefied pulverized liquid, and then the pulverized liquid was aged and stirred at 60 rpm for 13 hours for aging and fermentation. After aging and fermentation, the brownish brown liquid was filtered through a screen of 7 mesh to obtain a filtrate, and 4 parts by weight of nitrogen, 2 parts by weight of phosphorus, and 4 parts by weight of Kali were added and dissolved to prepare an amino acid solution.
이에 따라 제조된 아미노산 액비는 악취가 발생하지 않았다. 또한 4주일이 지나도 부패가 진행되지 않아 보존성이 뛰어남을 확인할 수 있었다.Amino acid liquor thus prepared did not cause odor. In addition, even after four weeks, corruption did not proceed, confirming the excellent retention.
본 발명의 제조방법은 생선폐기물, 수산가공 부산물, 양식폐사어류 등 각종의 수산 폐기물을 원료로 함으로써 환경적으로 심각한 문제가 되고 있는 수산 폐기물의 처리 문제를 해결함은 물론, 악취 발생의 문제 없이 값싼 원료를 사용하여 액비의 제조비용 또한 절약할 수 있으며 단백질 분해효소로서 서브틸리신을 사용함으로써 전처리 알칼리화 공정이 필요치 않게 되어 제조 공정이 간단하고 단시간이 소요되며 비용 또한 절약하면서 보존성이 뛰어난 양질의 아미노산 액비를 제조할 수 있다.The manufacturing method of the present invention solves the problem of disposal of marine waste, which is a serious environmental problem by using various marine waste such as fish waste, fish processing by-products, aquaculture waste fish, etc. By using raw materials, the manufacturing cost of liquid fertilizer can be saved as well. By using subtilisin as a protease, the pretreatment alkalizing process is not necessary. It can manufacture.
또한 본 발명의 방법에 의해 제조한 아미노산 액비는 생선뼈 등에 함유된 칼슘 및 인이 용출되어 액비 중에 수용성 칼슘 및 인으로 존재하므로 별도의 칼슘 및 인 성분을 시비하지 않아도 되며, 완제품의 아미노산 액비의 농도는 15～18％의 고농도로써 엽채류, 과채류의 엽면시비 및 간주용으로 1000～1500배 희석한 희석액으로도 식물의 맛과 향 증가, 색도 향상, 생산량 증가, 고온 및 냉해 예방, 병충해 감소 등의 탁월한 효과를 지닌다.In addition, the amino acid liquid fertilizer prepared by the method of the present invention does not have to fertilize separate calcium and phosphorus components because calcium and phosphorus contained in fish bones are eluted and present as water-soluble calcium and phosphorus in the liquid fertilizer. It is a high concentration of 15-18%, which is diluted 1000 ～ 1500 times for foliar fertilization and defoliation of leafy vegetables and fruits, and it is excellent in plant taste and aroma, color enhancement, yield increase, prevention of high temperature and cold, and reduction of pests. Has an effect.

Claims (6)

  1. 수산 폐기물을 용기에 담아 냉동시키는 냉동단계;A freezing step of freezing the seafood waste in a container;
    상기 냉동단계를 거친 냉동된 수산 폐기물을 파쇄한 후 분쇄하여 페이스트 상태의 분쇄물을 얻는 파쇄·분쇄단계; Crushing and pulverizing the pulverized frozen fishery waste which has undergone the freezing step and then pulverizing to obtain a pulverized product in a paste state;
    상기 파쇄·분쇄단계를 거친 페이스트 상태의 분쇄물을 발효조에 투입하고 단백질 분해효소 및 물을 첨가한 후, 35~60℃의 온수로 중탕하고 100~120rpm으로 15~20시간 동안 1차 교반하여 액화된 분쇄액을 얻는 액화단계;After the crushed and pulverized step of the pulverized product is added to the fermenter, the proteolytic enzyme and water is added, and then liquefied by hot water at 35 ~ 60 ℃ hot water and first stirred for 15 to 20 hours at 100 ~ 120rpm A liquefaction step of obtaining a pulverized liquid;
    상기 액화단계를 거친 분쇄액을 40~80rpm으로 10~15시간 동안 2차 교반하여 숙성, 발효시키는 발효단계;Fermentation step of aging, fermentation of the crushed liquid through the liquefaction step at 40 ~ 80rpm for 10-15 hours by secondary stirring;
    상기 발효단계를 거친 분쇄액을 여과하여 여액을 얻는 여과단계; 및A filtration step of filtrating the filtrate by filtering the milled liquid passed through the fermentation step; And
    상기 여과단계를 거친 여액을 교반기가 부착된 탱크로 이송한 후 질소(N), 인(P), 칼리(K), 붕소(B), 아연(Zn), 철(Fe), 고토(Mg), 몰리브덴(Mo), 망간(Mn) 중 어느 하나 이상을 첨가하여 용해시키는 용해단계를 포함하는 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비의 제조방법.The filtrate passed through the filtration step to a tank equipped with a stirrer and then nitrogen (N), phosphorus (P), kali (K), boron (B), zinc (Zn), iron (Fe), goto (Mg) , Molybdenum (Mo), Manganese (Mn) by adding any one or more of the dissolution step of dissolving the production of environmentally friendly amino acid liquid using aquatic waste.
  2. 제1항에 있어서, The method of claim 1,
    상기 단백질 분해효소는 서브틸리신인 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비의 제조방법.The proteolytic enzyme is a method for producing environmentally friendly amino acid liquid using aquatic waste, characterized in that the subtilisin.
  3. 제1항에 있어서, 상기 액화단계는,The method of claim 1, wherein the liquefaction step,
    수산 폐기물 100 중량부를 기준으로 물을 5~15 중량부, 단백질 분해효소를 0.5~3 중량부로 첨가하는 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비의 제조방법.A method for producing eco-friendly amino acid liquid fertilizer using aquatic waste, characterized in that 5 to 15 parts by weight of water, 0.5 to 3 parts by weight of protease is added based on 100 parts by weight of marine waste.
  4. 제1항에 있어서, 상기 여과단계는,The method of claim 1, wherein the filtration step,
    5~10메쉬(mesh)의 스크린 망으로 여과하는 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비의 제조방법.A method for producing an eco-friendly amino acid liquid fertilizer using a fishery waste, characterized in that filtered through a screen network of 5 ~ 10 mesh (mesh).
  5. 제1항에 있어서, 상기 용해단계는,The method of claim 1, wherein the dissolving step,
    질소 2~6 중량부, 인 0.5~3 중량부 및 칼리 2~6 중량부를 첨가한 후 용해시키는 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비의 제조방법.A method for producing an eco-friendly amino acid liquid ratio using a fishery waste, characterized in that after adding 2 to 6 parts by weight of nitrogen, 0.5 to 3 parts by weight of phosphorus and 2 to 6 parts by weight of Kali.
  6. 제1항 내지 제5항 중 어느 한 항에 의한 제조방법으로 제조되는 것을 특징으로 하는 수산 폐기물을 이용한 친환경 아미노산 액비.Eco-friendly amino acid liquid rain using aquatic waste, characterized in that produced by the manufacturing method according to any one of claims 1 to 5.
PCT/KR2011/000403 2010-03-05 2011-01-20 Amino acid liquid fertilizer using marine waste, and preparation method thereof WO2011108810A2 (en)

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CN103880482A (en) * 2014-03-21 2014-06-25 国农生物肥料连云港有限责任公司 Preparation method of crop organic fertilizer
CN110683908A (en) * 2019-10-31 2020-01-14 内蒙古阜丰生物科技有限公司 Amino acid slow-release fertilizer prepared from fermentation waste
CN112794757A (en) * 2021-03-26 2021-05-14 山东高晟联合农业科技有限公司 Production process of suspended organic water-soluble fertilizer

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KR102503986B1 (en) * 2021-02-10 2023-02-27 제주대학교 산학협력단 Method for manufacturing fermented liquid fertilizer of waste fish with reduced odor and fermented liquid fertilizer of waste fish produced by the same method

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CN103880482A (en) * 2014-03-21 2014-06-25 国农生物肥料连云港有限责任公司 Preparation method of crop organic fertilizer
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CN110683908A (en) * 2019-10-31 2020-01-14 内蒙古阜丰生物科技有限公司 Amino acid slow-release fertilizer prepared from fermentation waste
CN112794757A (en) * 2021-03-26 2021-05-14 山东高晟联合农业科技有限公司 Production process of suspended organic water-soluble fertilizer

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