KR101835200B1 - Cosmetic Composition for Improving Atopic Dermatitis Comprising Extract of Monochoria Korsakowii - Google Patents

Abstract

The present invention relates to a cosmetic composition for improving atopic dermatitis, which is characterized by containing a watercress extract as an active ingredient.
The cosmetic composition for improving atopic dermatitis according to the present invention exhibits an antiallergic, antioxidant, and anti-allergic effect to improve atopic dermatitis by containing watercress extract as an active ingredient.

Description

TECHNICAL FIELD [0001] The present invention relates to a cosmetic composition for improving atopic dermatitis,

The present invention relates to a cosmetic composition for improving atopic dermatitis containing a watercress extract.

Atopy occurs mainly in infancy and is called as fever, and it occurs mainly as the content of ceramides in the stratum corneum is decreased.

The cause of atopic dermatitis has not been clarified yet, but it is presumed that it is caused by the disturbance of the immune system.

In general, atopic dermatitis is dry due to a decrease in the water retention function of the stratum corneum, and skin barrier function is lowered, so that skin allergy can easily occur, so that it is prone to be pruritic, repeatedly recurring, do.

In many cases, it is exacerbated repeatedly at intervals of 1 to 2 weeks. If not properly managed, it progresses to subacute lesions of erythematous eruption with exudation, chronic lesions of thickening skin like skin.

The cause of atopic skin is divided into two main causes. One is genetic cause, and it is inherently deficient in gamma-linoleic acid (GLA) This is caused by the weak and easily disappearing of moisture on the surface of the skin. Other causes are environmental factors, such as dry living environment, reactive oxygen species, bacteria, viruses, fungi, etc. [Dermatology, Korean Society of Dermatology Editorial Committee,

Atopic dermatitis causes mast cells to release an allergic reaction inducer such as histamine in the intracellular granules, indicating degranulation [Gao ZG, et al., Biochem Pharmacol. 2009. Nov 5]. In addition, atopic dermatitis has been reported to be associated with allergic rhinitis and asthma, and thus it is highly likely to be an allergic disease [de Meer G, et al., Pediatr Allergy Immunol. 2009. Nov 13].

A composition comprising as a main ingredient various natural substances for improving atopic dermatitis has been disclosed. In Korean Patent Publication No. 10-1418916, a composition for improving atopic dermatitis comprising an extract of Chrysantida or an active fraction isolated therefrom by chromatography as an active ingredient is disclosed Korean Patent Publication No. 10-1382115 discloses a food composition containing ginseng fruit extract as an active ingredient and improving atopic dermatitis or dryness. Korean Patent Publication No. 10-1083347 discloses a food composition containing a moss extract as an active ingredient A pharmaceutical composition for improving or treating atopic dermatitis is disclosed.

On the other hand, Monochoria korsakowii is an annual plant of water and is known as a herbal medicine called ugu or ukuga. Waterworms have the power to cure fever, constrict asthma, and cure detoxification and swelling (boils). Waterworms are widely used as medicines, and they are used externally by taking a bite, taking a fresh leaf, attaching it to the affected part, or grinding the dried part with powder and attaching it to the affected part.

These wormwoods are mainly used as medicines, and no examples of using them as a composition for improving atopic dermatitis have been reported yet.

The inventors of the present invention have confirmed the efficacy for improving atopic dermatitis when using an extract of watercress, a natural product, as an active ingredient, and studied a composition containing the same to complete the present invention.

Korean Patent Publication No. 10-1418916 Korean Patent Publication No. 10-1382115 Korean Patent Publication No. 10-1083347

It is an object of the present invention to obtain an effect of improving atopic dermatitis by containing watercress extract as an active ingredient.

In order to attain the above object, the cosmetic composition for improving atopic dermatitis according to the present invention is characterized by containing a waterworm extract as an active ingredient.

At this time, the watercourse extract may be an extract obtained by adding watercress to a mixture of C1-C4 alcohol or water and a C1-C4 alcohol, and may be an extract obtained by adding methanol to the extract.

The cosmetic composition for improving atopic dermatitis of the present invention is characterized by containing the watercress extract in an amount of 0.001 to 97% by weight, preferably 0.01 to 20% by weight, based on the total weight of the cosmetic composition.

As described above, the cosmetic composition for improving atopic dermatitis according to the present invention exhibits an anti-allergic and antioxidative effect and thus has the effect of improving atopic dermatitis by containing a water gecko extract as an active ingredient.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the results of analysis of inhibition of the secretion amount of β-hexosaminidase according to the concentration of watercress extract of the present invention. FIG.
2 is a graph showing the results of analysis of the ABTS radical scavenging ability according to the concentration of the watercress extract of the present invention.

As used herein, "atopic dermatitis" is defined as including any disease classified as atopic dermatitis in the art, regardless of whether it is directly or indirectly related to its occurrence.

In general, atopic dermatitis is classified into infantile atopic dermatitis, infantile atopic dermatitis, adult atopic dermatitis, and maternal atopic dermatitis according to the onset period or the object of the invention. In this specification, atopic dermatitis includes all types of atopic dermatitis .

As shown in the following experimental examples of the present invention, clinical tests were conducted on 100 subjects (total 4 groups) having randomly selected atopic dermatitis symptoms, and as a result, it was found that about 85 to 95% Respectively.

These results are obtained for randomly selected subjects. Thus, the composition for improving atopic dermatitis of the present invention may be effective for all types of atopic dermatitis regardless of the cause of atopic dermatitis.

The term "improvement" as used in the present invention means all actions in which the symptoms of atopic dermatitis are alleviated or changed by administration of the composition of the present invention.

Hereinafter, the present invention will be described in detail.

The present invention is characterized by containing a waterworm extract as an active ingredient.

The watercourse extract may be an extract obtained by adding a C1-C4 alcohol or a mixture of water and a C1-C4 alcohol to a dried waterbath. The C1 to C4 alcohols may be any one or more of methanol, ethanol, propanol, isopropanol, and butanol. The alcohol may be used as a mixed solvent mixed with water.

In one preferred embodiment of the present invention, methanol is used for the extraction.

Specifically, the watercourse extract is cut into 2 to 3 cm by using a pencil. The cut worms are dried at a temperature of 30 to 50 ° C for 2 to 4 days and then 75 to 85% by weight methanol is added to the dry matter weight of 400 to 600 g, extracted using an ultrasonic device, and filtered.

The watercourse extract of the present invention is used as an effective ingredient of a cosmetic composition for improving skin wrinkles, wherein the watercourse extract may be contained in an amount of 0.001 to 97% by weight, preferably 0.01 to 20% But is not limited thereto.

In the case of the cosmetic composition, the water-in-oil extract is used as an active ingredient, and the emulsion, suspension, microemulsion, microcapsule, emulsion, or the like obtained by dispersing the oil phase in the aqueous phase, In the form of microgranules or ionic forms (liposomes), non-ionic follicular dispersions, creams, skins, lotions, powders, ointments, sprays or conical sticks. It can also be prepared in the form of a foam or an aerosol composition further containing a compressed propellant.

The cosmetic composition may further contain, in addition to the watercress extract of the present invention, a lipid, an organic solvent, a solubilizer, a thickener and a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a fragrance, Any of those commonly used in ionic or nonionic emulsifiers, fillers, sequestering and chelating agents, preservatives, vitamins, barrier agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics ≪ RTI ID = 0.0 > cosmetic < / RTI >

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples.

However, the following Examples and Experimental Examples are merely illustrative of the present invention, and the contents of the present invention are not limited by Examples and Experimental Examples.

<Examples>

Watercress extract was prepared as follows.

All solvents used were analytical grade, purchased from Sigma-Aldrich (USA) or Merck (USA).

The outpost of waterworms was cut evenly at 2.54 cm using a cutting blade and then dried in a drying oven at 40 ° C for 3 days. The outgrowths of about 500 g plant weight were extracted with 80 wt% methanol using an ultrasonic device. These liquids were filtered through a membrane filter (0.2 μm), and the solvent was removed in a vacuum to leave a methanol extract. Through these processes, the cellulose component of the waterworm was filtered through the purification process, and the waterworm extract became a methanol extract mainly containing trace components of waterworm.

<Experimental Example 1> Evaluation of anti-allergy effectiveness

Anti-allergic efficacy was assessed by measuring the amount of β-hexosaminidase. The inhibition of β-hexosaminidase secretion is an index indicating the degree of prevention of deaggregation of immune cells, and the results of the analysis are shown in FIG.

The experiment was carried out as follows.

First, Rat basophlic leukemia cell line in RBL-2H3 cell line 15% FBS, 100 unit / ml of penicillin and streptomycin MEM medium containing hygromycin and 37 ℃, 2 × 10 ⁵ cells on a 24 well plate in 5% CO ₂ conditions / well and cultured for 24 hours. After removal of the supernatant, MEM medium containing 25 ng / ml anti-DNP IgE was added, incubated for 4 hours, washed twice with PIPES buffer, and PIPES buffer containing 4 mM MgCl ₂ , 5.6 mM glucose and 0.1% Min.

Next, the samples were treated with 25, 50, and 100 μg / ml of 20 ng / ml of DNP-BSA and reacted for 20 min. After the reaction was terminated by ice for 10 minutes, the supernatant was added to a 96-well plate, and 1 mM P-nitrophenyl-acetyl-β-D-glucosaminyl was added thereto and reacted at 37 ° C. for 1 hour. Next, stop solution (0.1 M NaHCO ₃ , 0.1 M Na ₂ CO ₃ ) was added, and the absorbance was measured with an ELISA reader at 405 nm wavelength band. Ketotifen (100 ㎍ / ml) was used as a positive control.

The 50% inhibitory concentration (IC ₅₀ ) according to the measurement is shown in Table 1.

IC ₅₀ ([mu] g / ml) Watercress extract 46.79 Ketotifen 38.97

As a result of the cytotoxicity evaluation, it was confirmed that the waterweed extract of the present invention showed a much higher cell survival rate than the positive control group and thus the cytotoxicity was low.

1, it was confirmed that the effect of inhibiting degranulation is exhibited depending on the concentration of the watercress extract of the present invention. These results show the antiallergic effect of the watercress extract of the present invention, and the effect of improving atopic dermatitis when used as a cosmetic composition can be expected.

<Experimental Example 2> Evaluation of antioxidant effectiveness

Antioxidant efficacy was evaluated by measuring ABTS Latacian scavenging ability.

The ABTS radical scavenging ability was evaluated as follows, and the results are shown in Fig.

The reaction mixture was prepared by mixing 7 mM ABTS buffer and 2.45 mM potassium persulfate buffer (K ₂ S ₂ O ₈ ), mixing ABTS: K ₂ S ₂ O ₈ in a volume ratio of 2: 1 and shading for 12 to 16 hours. Next, the stock buffer was diluted so that the OD value measured at 734 nm was 0.70 ± 0.02, 100 μl of the diluted extract sample was added per 100 μl concentration of the buffer, and the mixture was reacted at room temperature for 6 minutes. The absorbance was measured at 734 nm and the IC ₅₀ value for each sample was determined and evaluated. BHA, an antioxidant, was used as a positive control.

The 50% inhibitory concentration (IC ₅₀ ) according to the measurement is shown in Table 2.

IC ₅₀ ([mu] g / ml) Watercress extract 88.85 BHA 3.37

As a result of the cytotoxicity evaluation, it was confirmed that the waterweed extract of the present invention showed a much higher cell survival rate than the positive control group and thus the cytotoxicity was low.

The results of FIG. 2 indicate that the ABTS radical scavenging activity tends to increase according to the concentration of the watercress extract of the present invention.

As described above, the watercress extract of the present invention showed the effect of anti-allergy and antioxidation. From these results, it was expected that the watercore extract of the present invention would be effective for improving atopic dermatitis when used as an active ingredient of a cosmetic composition.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It is possible to carry out various changes in the present invention.

Claims (5)

An antiallergic composition comprising Monochoria korsakowii extract as an active ingredient.
The method according to claim 1,
Wherein the composition for anti-allergy has a use for improving atopic dermatitis.
The method according to claim 1,
Wherein the watercourse extract is an extract obtained by adding watercress to a C1-C4 alcohol or a mixture of water and a C1-C4 alcohol.
4. The method according to any one of claims 1 to 3,
Wherein the composition is a cosmetic composition.
An antioxidant cosmetic composition comprising Monochoria korsakowii extract as an active ingredient.

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