KR101796689B1 - 안정화된 면역글로불린가변도메인 선별방법 및 선별된 도메인의 응용 - Google Patents

안정화된 면역글로불린가변도메인 선별방법 및 선별된 도메인의 응용 Download PDF

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KR101796689B1
KR101796689B1 KR1020167015418A KR20167015418A KR101796689B1 KR 101796689 B1 KR101796689 B1 KR 101796689B1 KR 1020167015418 A KR1020167015418 A KR 1020167015418A KR 20167015418 A KR20167015418 A KR 20167015418A KR 101796689 B1 KR101796689 B1 KR 101796689B1
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KR20160072272A (ko
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임형권
김성근
박영섭
남효정
김동식
박재찬
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재단법인 목암생명과학연구소
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G01N33/6857—Antibody fragments
    • C—CHEMISTRY; METALLURGY
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • G—PHYSICS
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    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C—CHEMISTRY; METALLURGY
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    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
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    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
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    • C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
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    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
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    • C40B40/04—Libraries containing only organic compounds
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    • G01N2500/00—Screening for compounds of potential therapeutic value

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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KR1020167015418A 2012-08-22 2012-08-22 안정화된 면역글로불린가변도메인 선별방법 및 선별된 도메인의 응용 Active KR101796689B1 (ko)

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PCT/KR2012/006680 WO2014030780A1 (en) 2012-08-22 2012-08-22 Screening and engineering method of super-stable immunoglobulin variable domains and their uses

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KR1020157004214A Active KR101732552B1 (ko) 2012-08-22 2012-08-22 안정화된 면역글로불린가변도메인 선별방법 및 선별된 도메인의 응용

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US (1) US10078085B2 (https=)
EP (2) EP3165535B1 (https=)
JP (1) JP6073038B2 (https=)
KR (2) KR101796689B1 (https=)
CN (3) CN113234142B (https=)
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Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014030780A1 (en) * 2012-08-22 2014-02-27 Mogam Biotechnology Research Institute Screening and engineering method of super-stable immunoglobulin variable domains and their uses
RS61778B1 (sr) 2013-05-06 2021-06-30 Scholar Rock Inc Kompozicije i postupci za modulaciju faktora rasta
GB201419899D0 (en) 2014-11-07 2014-12-24 Univ Kent Canterbury Biopharmaceutical production method
US9879087B2 (en) 2014-11-12 2018-01-30 Siamab Therapeutics, Inc. Glycan-interacting compounds and methods of use
FI3218005T3 (fi) 2014-11-12 2023-03-31 Seagen Inc Glykaanin kanssa vuorovaikutteisia yhdisteitä ja käyttömenetelmiä
KR101692629B1 (ko) * 2015-07-14 2017-01-03 계명대학교 산학협력단 매시브 플래카드 제어 장치 및 그 방법
CA3002097A1 (en) 2015-11-12 2017-05-18 Siamab Therapeutics, Inc. Glycan-interacting compounds and methods of use
AU2017259821B2 (en) * 2016-05-04 2024-07-18 Abilita Bio, Inc. Methods and platform for preparing multispanning membrane proteins
WO2018094143A1 (en) 2016-11-17 2018-05-24 Siamab Therapeutics, Inc. Glycan-interacting compounds and methods of use
WO2018160909A1 (en) 2017-03-03 2018-09-07 Siamab Therapeutics, Inc. Glycan-interacting compounds and methods of use
EP3459968A1 (en) * 2017-09-20 2019-03-27 Numab Innovation AG Novel stable antibody variable domain framework combinations
CN111201243B (zh) 2017-09-29 2023-08-11 财团法人牧岩生命科学研究所 对bcma具有高亲和力的抗bcma抗体和包含其的用于治疗癌症的药物组合物
KR102115300B1 (ko) * 2018-06-01 2020-05-26 재단법인 목암생명과학연구소 항체 라이브러리 및 이를 이용한 항체 스크리닝 방법
CN108893487A (zh) * 2018-07-19 2018-11-27 中国农业科学院北京畜牧兽医研究所 一种含有C-Myc蛋白融合标签的植物表达质粒载体及其载体的构建方法
WO2020127993A1 (en) * 2018-12-21 2020-06-25 Vib Vzw Fusion protein with a toxin and scaffold protein

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007016591A2 (en) * 2005-08-01 2007-02-08 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR960007784B1 (ko) 1993-07-02 1996-06-12 포항종합제철 주식회사 일산화탄소(co)가스 센서 및 그 제조방법
IL127127A0 (en) * 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
JP2002531113A (ja) * 1998-12-10 2002-09-24 ワシントン大学 蛋白質の形質導入システムおよびその使用法
WO2001018058A2 (en) * 1999-09-07 2001-03-15 Viventia Biotech Inc. Enhanced phage display library of human vh fragments and methods for producing same
US8372954B2 (en) * 2000-12-22 2013-02-12 National Research Council Of Canada Phage display libraries of human VH fragments
JP2005289809A (ja) 2001-10-24 2005-10-20 Vlaams Interuniversitair Inst Voor Biotechnologie Vzw (Vib Vzw) 突然変異重鎖抗体
JP2006508638A (ja) * 2002-05-22 2006-03-16 エスバテック・アーゲー 細胞内環境において向上した安定性を示す免疫グロブリンフレームワークおよびそれを同定する方法
GB0226729D0 (en) 2002-11-15 2002-12-24 Medical Res Council Intracellular antibodies
JP2006517789A (ja) * 2003-01-10 2006-08-03 アブリンクス エン．ヴェー． 治療用ポリペプチド、その相同物、その断片、および血小板媒介凝集の調節での使用
WO2005005604A2 (en) * 2003-06-30 2005-01-20 Centocor, Inc. Engineered anti-target immunoglobulin derived proteins, compositions, methods and uses
UA89481C2 (uk) 2003-09-30 2010-02-10 Центокор, Инк. Еритропоетинові міметичні шарнірно-серцевинні міметитіла людини, композиції, способи та застосування
US7973139B2 (en) * 2004-03-26 2011-07-05 Human Genome Sciences, Inc. Antibodies against nogo receptor
WO2006026759A2 (en) 2004-09-03 2006-03-09 Genentech, Inc. Humanized anti-beta7 antagonists and uses therefor
EP2360473A1 (en) * 2005-08-22 2011-08-24 Cornell Research Foundation Compositions and methods for analyzing protein interactions
EP2441838A3 (en) 2006-01-24 2013-07-10 Domantis Limited Fusion proteins that contain natural junctions
EP1981979A4 (en) 2006-01-31 2009-07-29 Republic Of Korea Republic Of PROCESS FOR PRODUCING A SOLUBLE NATIVE FORM OF RECOMBINANT PROTEIN USING THE SIGNAL SEQUENCE AND A SECRETION ENHANCING AGENT
TW200812616A (en) 2006-05-09 2008-03-16 Genentech Inc Binding polypeptides with optimized scaffolds
CN101720368A (zh) * 2007-03-09 2010-06-02 中国抗体制药有限公司 储备多样性最大化的功能性人化抗体文库之构建及应用
KR20100024943A (ko) * 2007-06-18 2010-03-08 다니스코 유에스 인크. 원핵생물에서의 단백질 생성을 위한 ｔａｔ 신호 펩타이드
WO2009089295A2 (en) * 2008-01-07 2009-07-16 Government Of The United States Of America, As Represented By The Secretary, Dept. Of Health And Human Services Anti-hiv domain antibodies and method of making and using same
JP6093715B2 (ja) 2011-01-28 2017-03-08 ナショナル リサーチ カウンシル オブ カナダ 免疫グロブリンドメインの工学的作製
WO2014030780A1 (en) * 2012-08-22 2014-02-27 Mogam Biotechnology Research Institute Screening and engineering method of super-stable immunoglobulin variable domains and their uses

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007016591A2 (en) * 2005-08-01 2007-02-08 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Annu Rev Microbiol. 2006;60:373-95
Protein Expr Purif. 2012 Jul;84(1):167-72

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JP6073038B2 (ja) 2017-02-01
CN106986934A (zh) 2017-07-28
WO2014030780A1 (en) 2014-02-27
KR20150032337A (ko) 2015-03-25
CN113234142B (zh) 2024-03-05
CN104769113B (zh) 2017-10-31
US10078085B2 (en) 2018-09-18
KR20160072272A (ko) 2016-06-22
EP3165535A1 (en) 2017-05-10
CN106986934B (zh) 2021-09-14
JP2015531597A (ja) 2015-11-05
EP2888367B1 (en) 2020-04-29
EP3165535B1 (en) 2019-05-15
EP2888367A1 (en) 2015-07-01
KR101732552B1 (ko) 2017-05-08
CN104769113A (zh) 2015-07-08
EP2888367A4 (en) 2016-04-20
US20160003844A1 (en) 2016-01-07
CN113234142A (zh) 2021-08-10

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