KR101784160B1 - Composition for moisturizing Skin comprising Maysin or Corn-silk extract - Google Patents
Composition for moisturizing Skin comprising Maysin or Corn-silk extract Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
Abstract
The present invention relates to a composition for skin moisturizing comprising corn beard extract (NICS-1) or macine isolated from corn beard, wherein the composition for moisturizing the skin comprising corn bean extract (NICS-1) It has been confirmed that not only stimulates growth of keratinocyte and promotes synthesis of hyaluronic acid but also does not stimulate skin, it can be used variously as a cosmetic composition or a pharmaceutical composition for skin moisturization, strengthening of skin barrier and prevention and treatment of keratinous skin .
Description
The present invention relates to a composition for skin moisturizing comprising corn beard extract (NICS-1) containing at least 70% of maize bean-derived meiocin and / or mexine as a single substance, and more particularly to a composition for skin moisturizing comprising corn beard- NICS-1), or a single substance, has been found to not only increase the synthesis of hyaluronic acid but also have a skin moisturizing effect without skin irritation, so that it can be manufactured and applied as a cosmetic composition for skin moisturizing.
Skin is divided into three layers, epidermis, dermis and subcutaneous fat tissue in order from the outside. When we look at the structure of skin, outer epidermis is functionally divided from outside to inside in stratum corneum, transparent layer, granular layer and basal layer , The inner dermis (dermis) is a tight connective tissue, and hypodermis inside the dermis (hypodermis) to fix the skin to the internal organs. In addition, the skin has about 65 ~ 70% of the human body's moisture, which plays an important role in helping the skin maintain its soft and moist condition.
It has been reported that the skin layer of the epidermis must have an essential moisture content of 10% or more, and the thickness of the stratum corneum varies depending on the content. The cells that make up this stratum corneum have a high concentration of Natural Moisturizing Factor (NMF), which is a water-soluble component, which helps the skin to maintain flexibility and maintain proper moisture. For example, The material is not only water-soluble, but also effectively combines with moisture to inhibit moisture from drying out of the skin.
Conventionally, a method of increasing water retention has been widely used by using a humectant having a property of absorbing moisture as a humectant and an occlusive moisturizer preventing evaporation of water. In addition, the formulations containing the lamellar liquid crystals, which have been undergoing much research until recently, are known to have excellent moisture retention due to their similar merits to the keratinous structures of the skin, but they can be manufactured only in a specific composition of the raw materials and thus have problems in universal use.
Currently, alcohols, water-soluble polymers and organic acid salts are used as moisturizers in pharmaceuticals and cosmetics (Imokawa G et al., J. Invest. Dermatol., 996: 523, 1991). However, these moisturizing materials have various problems. For example, glycerol and sorbitol, which are water-soluble polyhydric alcohols, have problems such as stickiness and stickiness compared with excellent moisturizing properties, and they have problems such that sodium lactate or urea, which is an organic acid, is highly electrolytic and deteriorates emulsification stability . Therefore, there is still a demand for not only efficacy but also feeling of use and formulation stability.
Recently, studies related to moisturizing functional materials have attracted much interest in research on the development of various types of materials using vegetable raw materials including herbal medicines in line with the tendency toward environment friendly. In general, a skin moisturizing cosmetic material such as a skin moisturizing cosmetic using a substance obtained by extracting various plants such as cucumber extract, carrot extract, green tea extract, safflower seed extract, mashin extract, ginkgo biloba extract, Lotions, creams, essences, and packs.
Korean Patent No. 10-0508379 discloses a skin-moisturizing functional cosmetic composition containing a polysaccharide extracted from a fruit of interest, and Korean Patent Publication No. 1992-0010271 discloses a skin moisturizing functional cosmetic composition containing a lactic acid fermented stock solution , Patent No. 10-1125878 discloses a skin moisturizing functional cosmetic composition containing a sweet complex, and Patent No. 10-0193105 discloses a skin moisturizer containing an extract of a herbal medicine such as jade, lily and the like. In addition, Japanese Patent Registration No. 10-0787312 discloses a moisturizing functional cosmetic composition containing jasmine, arnica, and Yarrow extract.
In general, maysin is known to be contained in corn, especially corn beard. Examples of the flavonoids derived from corn hairs include meisin, apimaysin, methoxymaysin and the like. Meishin is a typical substance contained specifically in corn beard, and is a larva of a moth pest, antioxidative activities such as inhibition of growth of corn earworm, cytotoxic effect on tumor cell line and radical scavenging activity have been reported. However, studies on the mechanism of action and various physiological functions in cells and in the body have been reported only in Korea Not all of them are done in the world.
Meishin is a stable substance in oxidation in the natural state and its effect is mainly attributed to O-dihydroxy located in the B ring of aglycone. Especially, the antibiotic effect of meicin is mainly caused by the aglycone, (luteolin).
In the prior art, it has been known that meisin has various physiological activities such as lipid metabolism improvement effect, anti-obesity, anti-cancer, immunity enhancement, anti-inflammatory and allergic asthma, anti-dementia (Korea Patent No. 1201628; Shun- Cheng Ren et al., Journal of Medicinal Plants Research, 3 (12): 1009, 2009), and skin moisturizing effects on mexin single substances have not been studied.
Thus, in the present invention, as a result of intensive efforts to develop a natural extract having no skin irritation as well as excellent skin moisturizing effect, a corn mustard extract containing 70% or more of Meishin separated from a corn beard into a C18 column (NICS- 1) and a single substance, meisin, not only increased hyaluronic acid synthesis but also had skin moisturizing effect without skin irritation, and completed the present invention.
It is an object of the present invention to provide a composition for skin moisturizing comprising a corn beard extract (NICS-1) containing 70% or more of meicin separated from a corn beard as a C 18 column or a single substance, ≪ / RTI >
In order to achieve the above object, the present invention provides a cosmetic composition for skin moisturizing comprising as an active ingredient a corn beard extract (NICS-1) containing Meishin in an amount of 70% or more or a single substance, Meicin.
In addition, the present invention provides a cosmetic composition for improving or strengthening skin barrier damage comprising corn beard extract (NICS-1) containing Meishin in an amount of 70% or more or Mechine as a single substance as an active ingredient.
The present invention also provides a pharmaceutical composition for treating or ameliorating skin barrier damage comprising corn beard extract (NICS-1) containing Meishin in an amount of 70% or more or a single substance, mexine as an active ingredient.
Further, the present invention provides a pharmaceutical composition for preventing or treating keratin skin, comprising corn beard extract (NICS-1) containing Meishin in an amount of 70% or more or mexin as a single substance as an active ingredient.
The present invention further provides a cosmetic composition for skin moisturizing, cosmetic composition for strengthening skin and a pharmaceutical composition for treating or preventing skin barrier damage, which comprises Meishin mixed with corn beard extract (NICS-1) containing not less than 70% do.
According to a preferred embodiment of the present invention, the corn beard extract (NICS-1) containing more than 70% of the macrocin or the macrocin which is a single substance may be corn-derived.
According to another preferred embodiment of the present invention, the composition is used for promoting keratinocyte cell growth; And promoting the synthesis of hyaluronic acid. [0034]
According to another preferred embodiment of the present invention, corn beard extract (NICS-1) containing mezin in an amount of 70% or more or mexine, which is a single substance, may be contained in an amount of 0.0001 to 20 parts by weight per 100 parts by weight of the total composition .
According to another preferred embodiment of the present invention, from 100 to 100 parts by weight of corn beard extract (NICS-1), may be contained in an amount of 50 to 100 parts by weight.
According to another preferred embodiment of the present invention, the cosmetic composition may be used as a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, , A massage cream, and a spray.
According to another preferred embodiment of the present invention, the pharmaceutical composition is an external preparation for skin, which comprises powders, gels, ointments, creams, liquids, and aerosols aerosol, < / RTI >
The skin moisturizing composition comprising the corn beard extract (NICS-1) and / or a single substance, mexin, containing 70% or more of Meicin according to the present invention not only accelerates the growth of keratinocytes and promotes the synthesis of hyaluronic acid, It can be variously used as a cosmetic composition or a pharmaceutical composition for skin moisturization, strengthening of skin barrier and prevention and treatment of keratinous skin.
Fig. 1 is data showing the cytotoxicity of HaCaT cells, which are human keratinocytes, according to their macrocein concentration.
FIG. 2 is data showing the degree of hyaluronic acid formation according to the macrochemical treatment concentration in keratinocytes (HaCaT cells).
3 is data showing the degree of formation of hyaluronic acid according to the mixing ratio of corn beard extract and mexin in keratinocytes (HeCaT cell) (Negative control: positive control: N-Acetyl-D-Glucosamine treated group, 100 / 25 to 150: a composition in which 25 to 150 parts by weight of Meicin is mixed with 100 parts by weight of corn beard extract).
Hereinafter, the present invention will be described in more detail.
As described above, alcohols, water-soluble polymers, organic acid salts and the like are generally used as moisturizers currently contained in medicines and cosmetics. However, these moisturizing materials have various problems. To this end, interest in moisturizing agents derived from natural extracts has been increased .
Accordingly, the present invention has solved the above-mentioned problem by providing a composition for skin moisturizing comprising a corn beard extract (NICS-1) containing mezin in an amount of 70% or more and / or a single substance, mexin. As a result, it is possible to provide a composition capable of promoting growth of keratinocyte and promoting hyaluronic acid synthesis without skin irritation to enhance skin moisturization and skin barrier. Therefore, not only cosmetics for skin moisturization or skin barrier strengthening, Or a pharmaceutical composition for treatment.
Accordingly, the present invention relates to a composition comprising corn beard extract (NICS-1) containing 70% or more of meicin separated from a corn beard as a C 18 column or mexine, which is a single substance, as an active ingredient,
The composition is a cosmetic composition for skin moisturizing, a cosmetic composition for improving or strengthening skin barrier damage, a pharmaceutical composition for improving or treating skin barrier damage, or a composition for preventing or treating keratin skin.
The composition of the present invention may additionally comprise a "pharmaceutically acceptable salt ". Pharmaceutically acceptable salts are salts which are prepared without toxicity or with a small amount of acid or base. When the compound of the present invention is relatively acidic, the base addition salts may be obtained by contacting a neutral form of such compound with a sufficient amount of the desired base and an appropriate inert solvent. Pharmaceutically acceptable base addition salts include, but are not limited to, salts formed with sodium, potassium, calcium, ammonium, magnesium or organic amino. Where the compounds of the present invention are relatively basic, acid addition salts can be obtained by contacting neutral forms of such compounds with a sufficient amount of the desired acid and a suitable inert solvent. Pharmaceutically acceptable acid addition salts include those derived from organic acids such as propionic acid, isobutyric acid, oxalic acid, malic acid, malonic acid, benzoic acid, succinic acid, sueric, fumaric acid, mandelic acid, phthalic acid, benzenesulfonic acid, Phosphoric acid, monohydrogencarbonic acid, phosphoric acid, monohydrogenphosphoric acid, dihydrogenphosphoric acid, sulfuric acid, monohydrogensulfuric acid, hydrogen iodide, phosphorous acid, And the like, but are not limited thereto. But are not limited to, salts of amino acids such as arginate and analogs of organic acids such as glucuronic or galactunoric acids.
The mexin is a yellow plant pigment flavonoid-based substance found in corn, and is known to have antioxidant and anticancer activities.
The corn beard extract (NICS-1) containing more than 70% of the macronutrient of the present invention or the macrocin which is a single substance is not particularly limited as long as it can be conventionally prepared and / or purchased, but is preferably corn-derived, more preferably corn Beard-derived.
The purity and content of the corn bean extract (NICS-1) or meicin contained in the composition of the present invention are not particularly limited, but in the present invention, corn beard extract (NICS-1) containing 70% And may be contained in an amount of 0.0001 to 20 parts by weight, preferably 0.001 to 10 parts by weight, more preferably 0.01 to 5 parts by weight based on 100 parts by weight of the total composition.
The solvent of the composition is not particularly limited as long as it does not interfere with the moisturizing effect of meicin. When mexin is contained at a concentration lower than 0.0001 part by weight based on 100 parts by weight of the total composition, promotion of keratinocyte cell growth, promotion of hyaluronic acid synthesis And skin barrier improvement effect may be decreased. If the concentration is higher than 20 parts by weight, the efficacy of the concentration increase may not be remarkably improved or cytotoxicity may be caused.
In the present invention, the composition is used for promoting keratinocyte cell growth; And promoting the synthesis of hyaluronic acid, can enhance skin moisturization or skin barrier through at least one efficacy.
FIG. 1 shows cytotoxicity of HaCaT cells treated with 10 ppm to 100 ppm of human keratinocyte. As shown in FIG. 1, no cytotoxicity was observed by Meishin. Dependent growth of the keratinocytes.
In addition, when comparing the cytotoxicity or keratinocyte growth of mexin, a single substance, with NICS-1, it was found that the present myskine has superior keratinocyte growth promoting activity compared to corn bean extract (NICS-1) Respectively. In general, it is known that maize beard contains meicin, but there is no research on the ability to promote keratinocyte on a single substance of maesin.
Keratinocyte is a major constituent of the epidermis. It is found in all layers of the epidermis. Keratin formation is the main function, and the cells are strongly bound together by desmosomes . The keratinocytes undergo nuclear differentiation for several weeks and undergo various stages of differentiation, and eventually become stratum corneum composed of homoeopathic keratinized cells with thick and firm keratinous cell membranes. The outer layer of the epidermis, the outer layer, is composed of keratinocytes and extracellular lipid-based material that surrounds each cell, and functions as a skin barrier to protect the skin from mechanical damage and absorption of water-soluble substances and moisture loss from the external environment.
If the keratinocyte is not grown properly for a variety of reasons such as aging, the thickness of the epidermis becomes thinner as a result, and the function as a moisturizing function and skin barrier can not be properly performed.
That is, the composition comprising Meicin of the present invention can improve the thickness of the skin layer by promoting the growth of keratinocyte, so that the cosmetic composition for improving or strengthening skin barrier damage or the pharmaceutical composition for improving or treating skin barrier damage And can be used as an emulsion composition.
FIG. 2 shows data on the increase in hyaluronic acid (hyaluronan) synthesis of human keratinocyte HaCaT cells treated with NPS-1 and mexine at 10 ppm to 100 ppm, respectively, It was confirmed that the synthesis of hyaluronic acid increases in keratinocytes depending on the renal concentration.
Hyaluronic acid is a biosynthetic natural substance that exists in many skin areas such as animals. It is a complex polysaccharide composed of amino acid and uronic acid. It is a high molecular compound composed of N-acetylglucosamine and glucuronic acid. Hyaluronic acid is a hydrophilic substance because it has many hydroxyl groups (-OH), and it is known that it acts as a moisturizing effect in the skin of animals and the like. It is generally present in human skin and is widely used in cosmetics for moisturizing action.
It contains a lot of hyaluronic acid in the skin. It contains a lot of moisture in the skin and it not only improves the moisturizing power but also acts to reduce damage to the lower part of the skin when the skin elasticity and skin damage occurs. Therefore, when hyaluronic acid decreases, The amount of moisture contained in the water is reduced and the surface of the skin is dried.
In one aspect of the present invention, a nutritional cream containing Meishin is prepared to confirm that the cosmetic composition for skin moisturizing including Meishin actually strengthens skin moisturization and skin barrier, thereby to prevent skin irritation, moisturizing effect, The effect was confirmed.
First, a skin irritation test was conducted and it was judged according to the criteria according to Table 3. As a result, it was confirmed that there was almost no irritation or slight irritation (Table 4).
In one embodiment of the present invention, a nutritional cream containing Meishin is applied to a mouse that induces skin barrier damage. After 6 hours of application, TEWL is measured to measure the degree of decrease of TEWL to evaluate the degree of decrease of TEWL As a result of measuring the degree of restoration of the skin barrier function, it was confirmed that the recovery effect after the damage of the skin barrier was increased, and the nutritional cream containing Meishin was superior to the nutritional cream containing Meishin (Table 6).
In another embodiment of the present invention, after applying a nutritional cream containing Meishin in order to confirm the skin moisturizing effect, the amount of evaporation of moisture and the persistence of moisture were checked, and as a result, the cosmetic containing Meishin significantly decreased the amount of moisture evaporation, It was confirmed that the moisturizing effect was very excellent (Table 7 and Table 8).
In another embodiment of the present invention, it was confirmed that the nutritional cream containing meicin was significantly superior to the nutritional cream containing meicin (Table 9).
That is, the skin moisturizing composition or the composition for improving or strengthening the skin barrier comprising the corn beard extract (NICS-1) containing Meishin of the present invention in an amount of 70% or more of the present invention and Meissin as a single substance promotes the growth of keratinocytes Hyaluronic acid synthesis as well as enhancing the skin moisturizing ability and the recovery effect of the damaged skin barrier without skin irritation and confirming that it has an effect of reducing keratin.
The cosmetic composition of the present invention may be selected from the group consisting of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, cleansing oils, powder foundations, emulsion foundations, wax foundations, packs, massage creams and sprays , And may contain at least one active ingredient which exhibits the same or similar function in addition to meisin.
The cosmetic composition may be prepared in the form of a general emulsified formulation and a solubilized formulation. Examples of the emulsified formulations include nutritive lotions, creams, essences, and the like, and the solubilization formulations include softening longevity. Suitable formulations include, but are not limited to, solutions, gels, solid or paste anhydrous products, emulsions obtained by dispersing the oil phase in water, suspensions, microemulsions, microcapsules, microgranules or ionic (liposomes) A cream, a skin, a lotion, a powder, an ointment, a spray, or a conical stick. It may also be in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.
Furthermore, the cosmetic composition is not particularly limited as long as it is usually added to cosmetics. For example, it is possible to use other additives such as fatty substances, organic solvents, solubilizers, thickening and gelling agents, softening agents, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, Chelating agents, preservatives, vitamins, blocking agents, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles and the like.
In the present invention, the pharmaceutical composition is an external preparation for skin, which is selected from the group consisting of powders, gels, ointments, creams, liquids and aerosols And may contain at least one active ingredient which exhibits the same or similar function in addition to meisin.
In addition, another applicable form of the external preparation for skin of the present invention is a pharmaceutical topical preparation. The external preparation for skin of the present invention may be manufactured with topical preparations containing at least one pharmaceutically acceptable carrier in addition to mexin in order to have a skin moisturizing agent and skin barrier improving or strengthening effect. The pharmaceutically acceptable carrier may be a mixture of saline, sterilized water, Ringer's solution, buffered saline, dextrose solution, maltodextrin solution, glycerol, ethanol and one or more of these components. If necessary, an antioxidant, , And other conventional additives such as a bacteriostatic agent may be added. It may also be formulated into topical preparations such as ointments, creams, gels, skin emulsions, skin suspensions, patches or sprays according to a conventional method by additionally adding a diluent, a dispersant, a surfactant, a binder and a lubricant. Further, it can be suitably formulated according to each disease or ingredient, using appropriate methods in the art or by the methods disclosed in Remington's Pharmaceutical Science (recent edition), Mack Publishing Company, Easton PA. Further, the dosage of the external preparation for skin according to the present invention may vary depending on the patient's body weight, age, sex, health condition, diet, administration time, administration method, excretion rate and severity of disease.
The present invention relates to a cosmetic composition for skin moisturizing, comprising a corn beard extract (NICS-1) and mexin as an active ingredient and comprising Meishin in an amount of 35 to 110 parts by weight based on 100 parts by weight of corn beard extract (NICS-1) A cosmetic composition for strengthening, and a pharmaceutical composition for improving or treating skin barrier damage.
The composition comprising the corn beard extract (NICS-1) containing more than 70% of Meicin of the present invention and Meicin as a single substance can promote the synthesis of hyaluronic acid, It can also have the effect of reducing damage to the lower part of the skin when damaged.
In one embodiment of the present invention, a mixed composition comprising both NICS-1 and Meicin separated by a C 18 column as studied in the present invention shows a synergistic effect promoting the synthesis of hyaluronic acid (see Table 17) . In addition, it not only improves skin moisturization, but also has the effect of reducing the damage of the lower part of the skin when the skin is damaged. In particular, when 35 to 110 parts by weight of Meishin is contained per 100 parts by weight of corn beard extract, effective skin damaging inhibitory effect is shown. More preferably 50 to 100 parts by weight of Meishin relative to 100 parts by weight of corn musth extract.
Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
Preparation of Meishin and Corn Extracts
The skin moisturization and skin barrier was used to extract and separate material from corn stigmas to make a May God effectiveness for improving / strengthening, the material separated using a C 18 column from corn beard, RDA National Food Research Institute 70 (NICS-1) and NICS-1 were purified and used Meishin solids with high purity.
To compare the efficacy of mei gins, corn mustache extract (NICS-1) and meicin were prepared using C 18 column, respectively.
The corn beard samples were collected from the 'Gwangpyeong Ok' cultivar produced by the National Institute of Rural Development, Rural Development Administration. They were cut into 5 ~ 10 ㎝ size immediately after harvesting, and 95% A (Duksung Industry) were added and extracted at a temperature of 25 ℃ for 9 days in a place where no direct sunlight shines. The extract was subjected to primary filtration with a cotton pad, 3 filter paper and then concentrated under reduced pressure. When the filtrate was concentrated under reduced pressure, the heating temperature was 40 ° C and the temperature of the reflux condenser was 10 ° C to obtain a corn steep crude extract concentrated under reduced pressure.
The resulting corn steep base extract was C 18 (prepared C 18 (Waters, Milford, Mass., MA) powder, and the mobile phase was flowed by a slanting method of only 100% by weight of ethanol in an initial composition of 100% by weight of ultrapure water (NICS-1).
The corn mustard extract (NICS-1) obtained through the above process is re-injected into a C 18 column to obtain a high purity macromolecule, and a fraction containing a large amount of mexine is taken and concentrated under reduced pressure. I got high mechine solids.
Cytotoxicity of Meicin
In the present invention, the cell survival rate of corn mustard extract (NICS-1) containing mezin of 70% or more and mexin cytotoxicity was measured.
First, human keratinocyte HaCaT cells (Human Keratinocyte, HaCaT, ATCC) were cultured in DMEM containing 10% FBS (fetal bovine serum), 100 units / mL penicillin and 100 mg / mL streptomycin Dulbecco ' s modified Eagle ' s medium; WelGENE Inc., Korea) at 37 [deg.] C in an incubator containing 5% carbon dioxide.
The starch extract of corn (NICS-1) obtained in Example 1 and Meishin were prepared by preparing stock at a concentration of 200 mg / ml using distilled water and then adding to DMEM medium to which no FBS was added to prepare a sample solution Respectively.
Then, the HaCaT cells were seeded in a 96-well plate at a density of 5 × 10 4 cells / well and cultured in the same medium for 24 hours. Thereafter, the medium was removed and the starvation state was maintained for 12 hours. Then, the Meishin sample solution prepared above was used to treat the corn beard extract (NICS-1) and mexin at a concentration of 10 ppm, 50 ppm, and 100 ppm And then cultured for 24 hours.
Cell viability was determined by diluting the WST-1 reaction solution (Cell proliferation reagent WST-1, Roche, cat. No. 1644-807, Switzerland) to 1/10 in DMEM medium to which none was added, After reacting for 1 hour, the absorbance at 450 nm was measured, and the negative control group was used as a negative control.
All statistical analyzes were performed with SAS ver. 9.2 (SAS Institute, Cary, USA). The significance of the differences was determined using Duncan's multiple test and ANOVA, and a significant difference was assessed at p <0.05. All data were significant in the 5% range and were expressed as mean ± standard deviation.
As a result, as shown in Table 1 and FIG. 1, no cytotoxicity was observed by Meishin, which was isolated and purified from corn bean extract (NICS-1) and NICS-1 isolated on a C 18 column, (NICS-1) and meiocin concentration, the growth of keratinocytes was accelerated. That is, the composition comprising the corn musth extract (NICS-1) of the present invention and Meicin can improve the thickness of the skin layer by promoting the growth of keratinocytes, so that the cosmetic composition or skin May be utilized as a pharmaceutical composition for improving or treating barrier damage.
Confirming the ability of corn mustache extract (NICS-1) and meisin to promote the synthesis of hyaluronic acid
In the present invention, the degree of hyaluronan synthesis in keratinocytes was measured by ELISA to evaluate the skin moisturizing ability of corn beard extract (NICS-1) and Meishin isolated by C 18 column.
HaCaT cells and NICS-1 and macrocin were prepared in the same manner as in Example 2, and HaCaT cells were dispensed into 96-well plates at a density of 6 × 10 4 cells / well, And cultured in the same medium as above for 24 hours. Thereafter, the medium was removed and the concentration of NICS-1 was adjusted to 50 ppm and 100 ppm using the corn beard extract (NICS-1) prepared above and Meishin sample solution. Meishin was added at concentrations of 10 ppm, 50 ppm and 100 ppm And then cultured for 24 hours.
The medium of the cultured cells was collected and the amount of hyaluronic acid synthesized was measured using a hyaluronan ELISA (R & D system, DY3614, USA). On the other hand, the cells attached to the bottom were washed with PBS and lysed with 1N sodium hydroxide (NaOH) to measure total protein amount to calculate the amount of hyaluronic acid synthesis per a certain protein. N-Acetyl-D-Glucosamine (SIGMA, cat. No. A8625, USA), known to increase hyaluronic acid synthesis as a positive control, was used as a negative control. 20 mM.
As a result, Table 2, and as shown in FIG. 2, NAG used in the positive control group was confirmed that the hyaluronic acid of 190.8% compared to the control when 20 mM process generates, processes the NICS-1 separated by a C 18 column In contrast, at 50 ppm, the hyaluronic acid production was lower than that of the control group. However, at 100 ppm, the hyaluronic acid production was increased to 102.97% And 130.92% and 157.18% at 50 ppm and 100 ppm, respectively, indicating that the synthesis of hyaluronic acid was promoted by Meishin.
That is, since the composition comprising NICS-1 and Meicin isolated by the C 18 column as described in the present invention can promote the synthesis of hyaluronic acid, it is possible not only to improve skin moisturization, Can also have the effect of reducing.
Manufacture of cream formulations containing mexin as active ingredient
In the present invention, nutritional creams were prepared as shown in Table 3 below to prepare cosmetics containing actual makeup.
0.02 part by weight, 0.2 part by weight and 1.0 part by weight were added to 100 parts by weight of the total composition, and the group to which no addition was added was compared with the group containing 1.0 part by weight of cornstarch extract (NICS-1) Was used as Comparative Example 2.
Skin irritation experiment
To measure the skin irritation of the nutritional creams of Comparative Example 1, Comparative Example 2 and Formulation Examples 1 to 3 prepared in Example 4, the following methods were used.
Twenty healthy men and women were enrolled in the CTFA Guidelines (The Cosmetic, Toxic and Fragrance Association, Inc. Washington, DC, 20036, 1991). 15 mg / 15 μl of the sample and solution was dropped into a finn chamber, and this was placed on the arm of the human body to be tested and fixed with a tape. Then, the skin was reacted for 24 hours, and after removing the patch for 2 hours, the skin reaction of the test site was judged according to the criteria of Table 4 below.
As a result, as shown in Table 5, it was confirmed that Comparative Example 2 containing Formulation Examples 1 to 3 containing meisin and corn bean extract (NICS-1) had no skin irritation.
Confirmation of improvement of skin barrier damage
Acetone was applied as a method of damaging the skin barrier in order to confirm the skin barrier damage improvement and strengthening effect of the nutritional creams of Comparative Example 1, Comparative Example 2 and Formulation Examples 1 to 3 prepared in Example 4. When transepidermal water loss (TEWL) reached 4.0 g / m < 2 > / h by dropping acetone into the aliquots of 8- to 12-week old reared mice, the nutritional values of the Comparative Example 1, Comparative Example 2 and Formulations 1 to 3 Cream.
TEWL was measured with a Tewameter 210 evaporator from C + K (Cologne, Germany). After 6 hours of application, TEWL was measured and the degree of reduction of TEWL was measured to evaluate the degree of reduction of TEWL.
[Equation 1]
(Bt = 6 - Bt = 0) / (Bt = d - Bt = 0) * 100
Bt = 6: TEWL measurement value after 6 hours after skin barrier damage
Bt = 0: TEWL measurement before skin barrier damage
Bt = d: TEWL measured immediately after skin barrier damage
As a result, as shown in Table 6, while the nutritional cream containing corn beard extract (NICS-1) showed a recovery rate similar to that of Comparative Example 1, the nutritional cream containing Meishin showed recovery after skin barrier damage It was confirmed that the effect was increased.
Check skin moisturizing effect
35 adult male and female persons who were alleged to be dry or dry skin were applied twice daily with the nutritional creams of Comparative Example 1, Comparative Example 2 and Forms 1 to 3 prepared in Example 4 for 4 weeks to the inside of the toes. TEWL (Trans Epidermal Water Loss) was measured before and 2 hours after application and at 4 weeks after application, under constant temperature and humidity conditions (24 ℃,
As a result, as shown in Table 7, while the nutritional cream containing corn beard extract (NICS-1) showed a level of water evaporation similar to that of Comparative Example 1, the nutritional cream containing Meishin showed a water evaporation amount , And it was confirmed that it has a very excellent moisturizing effect.
In order to examine the moisturizing duration of each formulation, Comparative Example 1, Comparative Example 2 and Formulations 1 to 3 were applied once, and then the skin moisture was measured by a corneometer for 6 hours. The amount of water present in the epidermis of the skin was measured in terms of the degree of ion of water using a sensor, and the moisture content was calculated by calculating the water content.
As a result, as shown in Table 8, it was confirmed that Formulation Examples 1 to 3, which are nutritional creams containing meicin, have a significantly higher skin moisture content than the non-inoculum group, Comparative Example 1 and Comparative Example 2. That is, the cosmetic containing Meishin showed excellent moisturizing effect.
Each mass reduction effect
The skin erosion reduction amounts of the nutritional creams of Comparative Example 1, Comparative Example 2 and Formulation Examples 1 to 3 prepared in Example 4 were measured.
The nutritional creams of Comparative Example 1, Comparative Example 2 and Formulation Examples 1 to 3 were applied to 50 men and women in their 20s and 30s who had no skin disease. After 24 hours, the Charm view (Moritex, Japan ) Was used to measure the reduction of keratin.
The skin deadening amount was calculated by measuring the mass of the initial skin mass and the mass after 24 hours using the Charm view in a constant temperature and humidity condition (40% humidity) before the start of application, and the results are shown in Table 9 Respectively.
As a result, as shown in Table 9, it was confirmed that Formulation Examples 1 to 3, which are nutritional creams containing meicin, are significantly superior in the reduction of skin horny than Comparative Examples 1 and 2.
[Formulation Example 4] Flexible lotion
Flexible lotion was prepared by a conventional method according to the composition shown in Table 10 above.
[Formulation Example 5] Convergent lotion
A convergent lotion was prepared according to a conventional method according to the composition shown in Table 11 above.
[Formulation Example 6] Nutritional lotion
Nutrition lotion was prepared according to a conventional method according to the composition shown in Table 12 above.
[Formulation Example 7] Essence
The essence was prepared by a conventional method according to the composition shown in Table 13 above.
[Formulation Example 8] Massage cream
Massage creams were prepared in a conventional manner according to the composition shown in Table 14 above.
[Formulation Example 9] Pack
A pack was prepared by a conventional method according to the composition shown in Table 15 above.
[Formulation Example 10] Ointment for external preparation for skin
An ointment for external application for skin was prepared according to a conventional method according to the composition shown in Table 16 above.
The composition ratio of the above-mentioned formulation examples is a mixture of ingredients suitable for cosmetic or dermatological formulation, but it is also possible to arbitrarily modify the compounding ratio according to the local or national preference such as demand level, demand country, use purpose.
Synergistic effect of mixture of maize beard extract (NICS-1) and mexin
In the present invention, the degree of hyaluronan synthesis in keratinocytes was measured by ELISA to evaluate skin moisturizing ability in a composition comprising corn beard extract (NICS-1) and Meicin isolated on a C 18 column.
HaCaT cells and NICS-1 and macrocin were prepared in the same manner as in Example 2, and HaCaT cells were dispensed into 96-well plates at a density of 6 × 10 4 cells / well, And cultured in the same medium as above for 24 hours. Then, the medium was removed, and the maize beard extract (NICS-1) prepared above was mixed with Meichen. The mixing weight ratio was mixed with
The medium of the cultured cells was collected and the amount of hyaluronic acid synthesized was measured using a hyaluronan ELISA (R & D system, DY3614, USA). On the other hand, the cells attached to the bottom were washed with PBS and lysed with 1N sodium hydroxide (NaOH) to measure total protein amount to calculate the amount of hyaluronic acid synthesis per a certain protein. N-Acetyl-D-Glucosamine (SIGMA, cat. No. A8625, USA), known to increase hyaluronic acid synthesis as a positive control, was used as a negative control. 20 mM.
As a result, as shown in Table 17 and FIG. 3, it was confirmed that 190.8% of hyaluronic acid was produced at 20 mM when NAG was used as a positive control, and 25 parts by weight of MeOH extract The composition containing the gods showed 110.5% hyaluronic acid synthesis promoting ability. On the other hand, it was confirmed that hyaluronic acid synthesis promoting ability was 141.6%, 184.2%, and 190.2%, respectively, in the mixture of
That is, the mixed composition including both NICS-1 and Meicin separated by the C 18 column as discussed in the present invention can cause a synergistic effect of promoting the synthesis of hyaluronic acid, so that it is possible not only to improve skin moisturization, It can also have the effect of reducing damage to the lower part of the skin when damaged. In particular, when 35 to 110 parts by weight of Meishin is contained relative to 100 parts by weight of corn beard extract, the most effective skin damaging inhibitory effect is shown.
While the present invention has been particularly shown and described with reference to specific embodiments thereof, those skilled in the art will appreciate that such specific embodiments are merely preferred embodiments, It will be obvious. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.
Claims (15)
Wherein the composition is 0.001 part by weight to 10 parts by weight based on 100 parts by weight of the total composition.
Promoting keratinocyte cell growth; And
Promoting hyaluronic acid synthesis;
Wherein the moisturizing or skin barrier is strengthened by at least one selected from the group consisting of at least one of the following ingredients.
Promoting keratinocyte cell growth; And
Promoting hyaluronic acid synthesis;
Wherein the skin barrier is strengthened by moisturizing or skin barrier through at least one efficacy selected from the group consisting of: < RTI ID = 0.0 >
Wherein the macine is 35 to 110 parts by weight based on 100 parts by weight of the corn beard extract (NICS-1).
Wherein the macine is 35 to 110 parts by weight based on 100 parts by weight of the corn beard extract (NICS-1).
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| KR20190060651A (en) | 2017-11-24 | 2019-06-03 | 주식회사 엑소코바이오 | A composition comprising an exosome derived from stem cell as an active ingredient and its application for reinforcing or improving skin barrier |
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| CN107550815A (en) * | 2017-09-18 | 2018-01-09 | 上海家化联合股份有限公司 | A kind of maize seed extract and its application in skin preparations for extenal use |
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| CN103371359A (en) | 2012-04-12 | 2013-10-30 | 刘畅 | Preparation method for novel health-care nutrient |
| KR101535835B1 (en) | 2013-04-02 | 2015-07-10 | 광동제약 주식회사 | Composition for skin whitening containing corn silk extract |
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| CN103371359A (en) | 2012-04-12 | 2013-10-30 | 刘畅 | Preparation method for novel health-care nutrient |
| KR101535835B1 (en) | 2013-04-02 | 2015-07-10 | 광동제약 주식회사 | Composition for skin whitening containing corn silk extract |
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| KR20190060651A (en) | 2017-11-24 | 2019-06-03 | 주식회사 엑소코바이오 | A composition comprising an exosome derived from stem cell as an active ingredient and its application for reinforcing or improving skin barrier |
| KR20190060646A (en) | 2017-11-24 | 2019-06-03 | 주식회사 엑소코바이오 | A composition comprising an exosome and/or extracellular vesicle derived from stem cell as an active ingredient and its application for reinforcing or improving skin barrier |
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