KR101535835B1 - Composition for skin whitening containing corn silk extract - Google Patents

Abstract

The present invention relates to a composition for whitening skin, which comprises an extract of corn musth as an active ingredient. The composition of the present invention can inhibit the production of melanin in melanin-producing cells safely without cytotoxicity, suppress the expression of tyrosinase involved in melanin biosynthesis, and exert an excellent skin whitening effect by containing corn salad extract as an effective ingredient have.

Description

Technical Field [0001] The present invention relates to a skin whitening composition containing a corn scrub extract,

The present invention relates to a skin whitening composition containing a corn salad extract.

Melanin is produced against the damage of the skin caused by ultraviolet rays and protects the skin. The process of biosynthesis of melanin begins with the oxidation of tyrosine by tyrosinase. After the production of DOPA (3,4-dihydroxyphenylalanine) and dopachrome, DHICA (5,6 -Dihydroxyindole-2-carboxylic acid) and DHI (5,6-dihydroxyindole), and it is known that DHI-eumelanin, DHICA-eumelanin, and peomelanin are produced.

Specifically, melanin synthesis is mediated by the action of tyrosinase and tyrosinase related proteins present in the melanosome. Tyrosine is oxidized by tyrosinase to form DOPA, and then DOPA is oxidized again by the action of dopaoxidase to generate dopaquinone. Then, tyrosinase-related protein TRP-1 (5, 6-dihydroxyindole-2-carboxylic acid oxidase) and TRP-2 (dopachrome tautomerase). The expression of tyrosinase, TRP-1 and TRP-2 is promoted by a transcription factor MITF (microphthalmia-associated transcription factor). MITF is inhibited when ERK (extracellular signal regulated kinase) is activated. Therefore, a whitening effect can be expected when the expression of tyrosinase and / or MITF is inhibited or the expression of ERK is promoted.

Blackening of the skin occurs when melanin produced in melanocytes in skin cells is transferred to keratinocytes and accumulates in skin surface layer. Melanin pigmentation and deposition of the skin cause spots, freckles, and black spots. Kojic acid, arbutin, vitamin C derivatives and the like have been developed as a whitening agent in order to prevent and improve the blackening of such skin.

However, in the case of Koji acids in vitro (in vitro ) tyrosinase inhibitory effect compared with the cytosolic or in vivo ( in vivo of melanin synthesis inhibitory effect is not only small, but also can cause liver cancer when used for a long period of time. In addition, vitamin C derivatives have a problem of chemical stability, which makes it difficult to store them for a long period of time.

As mentioned above, it is known that side effects of whitening active materials have recently been investigated, and active whitening materials using natural extracts have been actively studied as alternative raw materials. For example, in Korean Patent No. 0864174, it has been suggested that ginseng, Angelica gigas, licorice, etc. have a whitening effect. In addition, grape, garlic, mushroom, black bean, Rice, wormwood, acorns, chestnut, acerola cherries, and beans.

It is a reality that the whitening composition using natural extracts so far has not been sufficiently verified for its efficacy and toxicity. Therefore, it is urgent to develop an alternative natural whitening agent that can demonstrate both safety and efficacy.

Accordingly, the present invention has been extensively studied in order to meet the above-mentioned development needs, and as a result, it has been discovered for the first time that there is skin whitening activity in corn beard extract, leading to the present invention.

The composition of the present invention can inhibit the production of melanin in melanin-producing cells safely without cytotoxicity, suppress the expression of tyrosinase involved in melanin biosynthesis, and exert an excellent skin whitening effect by containing corn salad extract as an effective ingredient have.

FIG. 1 is a graph showing cell viability and melanin production after three days of treatment with a mouse-derived melanin-producing cell line at 100 ppm concentration of corn mustard extract and arbutin.
FIG. 2 shows ECL detection results showing the amount of tyrosinase expression after 3 days treatment with corn beard extract and arbutin at concentrations of 1 ppm, 10 ppm and 100 ppm.
FIG. 3 is a graph showing the amount of corn beard extract at concentrations of 1 ppm, 10 ppm and 100 ppm and the amount of dopachrome produced after 3 days of treatment with arbutin at an absorption of 492 nm.
FIG. 4 shows changes in skin brightness (L * value) after 4 weeks and 8 weeks of application of corn beard extract (mean ± SEM, * P < 0.05). A: 0.75% aqueous solution of corn mustache extract / B: 1.5% aqueous solution of corn mustard extract
FIG. 5 shows changes in skin color (ITA ° value) after 4 weeks and 8 weeks of application of corn beard extract (mean ± SEM, * P <0.05). A: 0.75% aqueous solution of corn mustache extract / B: 1.5% aqueous solution of corn mustard extract
Fig. 6 shows a color image of the subject after 8 weeks of using a 1.5% aqueous solution of corn mustache extract.
FIG. 7 shows changes in area of hyperpigmentation sites after application of corn mustache extract for 4 weeks and 8 weeks (mean ± SEM, * P < 0.05). A: 0.75% aqueous solution of corn mustache extract / B: 1.5% aqueous solution of corn mustard extract
Figure 8 shows the change in density of hyperpigmented sites after application of corn mustache extract for 4 weeks and 8 weeks (mean ± SEM, * P < 0.05). A: 0.75% aqueous solution of corn mustache extract / B: 1.5% aqueous solution of corn mustard extract

Hereinafter, the present invention will be described in detail. However, it should be understood that the present invention may be embodied in many other specific forms without departing from the spirit or essential characteristics thereof.

A corn silk is a thread that is tangled at the end of corn, most of which is abandoned. However, recently, it has been known that the taste and flavor of corn beard is excellent, and it has also been marketed as a corn barrel, and it is also used as a spice and a food additive.

The present invention relates to a composition for whitening skin, which comprises an extract of corn musth as an active ingredient.

The corn used in the present invention is not particularly limited to the variety of the corn, but there are no particular restrictions on the type of corn used in the present invention. However, the corn used in the present invention is not particularly limited, Dahok, Jangdahok, Gwangpyeong, and the like are preferable. The name of the corn variety is based on the description of major crop varieties in 2011 by the National Institute of Food Science and Technology.

The corn beard extract may be prepared by extracting all or part of corn hairs. The corn musth extract can be extracted according to a conventional plant extract method such as hot water, warming, cooling, stirring, ultrasonic irradiation, and the like, and a hot water extraction method is preferable. Further, it can be extracted with at least one solvent selected from the group consisting of water, alcohol and organic solvent, and most preferably, it can be extracted with water, especially hot water. The organic solvent is not particularly limited as long as it can be used for extraction. As an example, the solvent may be extracted by adding 25 times the amount of purified corn base water. After the extraction with the solvent, a filtration process may be further performed, and the mixture may be pulverized through vacuum concentration, freeze-drying, or the like.

In one aspect, the present invention relates to a cosmetic composition for skin whitening comprising corn scratch extract as an active ingredient.

In the cosmetic composition of the present invention, the extract of corn musth may be contained in an amount of 0.001% to 20% based on the total weight of the composition, but the content can be appropriately adjusted depending on the content of the other ingredients contained in the formulation or cosmetic composition. However, when the content of the extract of corn hairs in the cosmetic composition is less than 0.001%, it may be difficult to expect a substantial whitening effect in the cosmetic composition, and when it exceeds 20%, it may be uneconomical to the expected efficacy. Therefore, it is preferable that the content is in the range of 0.001% to 20% based on the total weight of the composition.

In one embodiment of the present invention, the cosmetic composition of the present invention may further contain at least one cosmetically acceptable additive. The at least one additive that is acceptable for cosmetics is not particularly limited and may be selected from conventional ingredients incorporated in common cosmetics such as oil, water, a surfactant, a moisturizer, an alcohol, a thickener, a colorant, an antiseptic, a flavor, a chelating agent, , Sunscreen agents, fatty acids, and the like.

The cosmetic composition of the present invention may be any one selected from the group consisting of a skin, a lotion, a cream, a gel, an essence, a foundation, a lotion, a patch, a pack, a soap, a cleanser and a detergent without particular limitation.

In one embodiment of the present invention, the cosmetic composition of the present invention may contain other known compounds or plant extracts already known or later known to have a skin whitening effect so as to enhance or reinforce the skin whitening effect, Can be included together.

In another aspect, the present invention relates to a skin whitening food composition containing corn musth extract as an active ingredient.

In the food composition of the present invention, the corn salad may be contained in an amount of 0.001% to 20% based on the total weight of the composition, but the content can be appropriately adjusted according to the content of other ingredients contained in the food composition. However, if the content of the corn beard extract in the food composition is less than 0.001%, it may be difficult to expect a substantial whitening effect in the food composition, and if it exceeds 20%, it may be uneconomical to the expected efficacy. Therefore, it is preferable that the content is in the range of 0.001% to 20% based on the total weight of the composition.

In one embodiment of the invention, the food composition of the present invention may additionally contain one or more additives which are acceptable for food use. The at least one additive that is acceptable for foodstuffs is not particularly limited and may contain conventional ingredients that can be incorporated into foods, such as water, flavor, flavor, colorant, stabilizer, preservative, alcohol, Vitamins, plant extracts, organic acids, minerals, nutrients, and the like.

The form of the food composition of the present invention is not particularly limited, but may be any form selected from the group consisting of beverage, gum, tea, confectionery, vitamin complex and health supplement.

In one embodiment of the present invention, the food composition of the present invention may contain, in addition to the main ingredient corn musth extract, other known compounds or plant extracts already known or later to have a skin whitening effect so as to enhance or reinforce the skin whitening effect Can be included together.

In the present invention, skin whitening-related activity of corn beard extract, which is known as a food material, was measured. As a result, it was confirmed that melanogenesis inhibitory activity of melanin - producing cells was higher than that of arbutin, which is a single substance at the same concentration, without cytotoxicity.

In addition, corn beard extract significantly reduced tyrosinase expression in melanin - producing cells, which was also superior to arbutin.

In addition, application of the aqueous solution of corn mustache extract to female subjects with hyperpigmented calcification on the face showed significant skin color reduction effect without any adverse reaction.

Therefore, corn mustache extract is thought to reduce melanin production by inhibiting expression of tyrosinase. The corn mustache extract may be used as a stable skin whitening material to replace arbutin, which is the most commonly used whitening functional substance, which is recently used in cosmetic compositions.

The present invention will be described in more detail with reference to the following examples. However, the following examples are for the purpose of illustrating the present invention and are not intended to limit the scope of the present invention.

[Example]

Statistical analysis

Statistical significance of the data in this embodiment was analyzed using SPSS Package Program (manufactured by IBM, USA). The Shapiro-Wilks test was used for the normality test in the human body test. The point-by-point comparison was conducted using the repeated measures ANOVA for the parameters and the Wilcoxon's signed -ranks test).

Preparation of corn mustache extract

Corn beard was washed with running water and sterilized by heating at 135 ° C for 1 hour as a pretreatment. Twenty-five times the amount of purified water based on the corn hairs was added and the mixture was extracted at a temperature of 98 to 100 ° C or more for 1.5 hours. The extract was filtered using a mesh and concentrated at 60 ° C or lower. The stored concentrate was filtered again using a microfilter and sterilized by heating at 125 DEG C for 8 seconds.

Measurement of cell viability and melanin production of melanin-producing cells

The effect of corn mustache extract on cell survival and melanin production in mouse - derived melanin - producing cell lines was measured. The cells were cultured in RPMI 1640 medium containing 10% FBS (fetal bovine serum) and 1% penicillin-streplomycin and 200 nM of forbol-12-myristate 13-acetate under 37 ° C and 5% CO ₂ . Arbutin, which is a positive control substance, and arbutin, which is a positive control substance, were treated for 3 days at concentrations of 1 ppm, 10 ppm, and 100 ppm, respectively, and cell viability and melanin content were measured. The results are shown in Tables 1 and 2 below. The cell survival rate is expressed as a percentage of survival relative to the untreated cells.

Treated material Concentration (ppm) Melanin-producing cell viability (%)
Corn mustache extract
One 102.1 10 91.0 100 96.9
Arbutin
One 97.1 10 91.6 100 105.8

As shown in Table 1, it was confirmed that there was no significant change in the melanin-producing cell survival rate at all the concentrations treated with arbutin and corn bean extract.

Treated material Concentration (ppm) Melanin production (%)
Corn mustache extract
One 100.0 10 90.8 100 62.8
Arbutin
One 97.7 10 99.4 100 73.2

As shown in Table 2, it was confirmed that the treatment with corn beard extract had a lower amount of melanin than the treatment with the same concentration of arbutin.

The cell viability at 100 ppm and the amount of melanin production are shown in FIG. 1 at the same time to facilitate comparison.

Measurement of effect on tyrosinase expression

Western immunoblotting was performed to determine the effect of tyrosinase expression, which plays a major role in the melanin biosynthesis process. The corn mustache extract was treated with melan-a cells for 3 days and then extracted with lysis buffer. After separation using 8% SDS-polyacrylamide gel, ECL (enhanced chemiluminescence) was performed under conditions of tyrosinase primary antibody (1/250 dilution) and anti-goat secondary antibody (1/13000 dilution) ). The results are shown in Fig. As shown in Fig. 2, when corn musth extract was treated for 3 days at a concentration of 1 ppm, 10 ppm, and 100 ppm, the amount of tyrosinase expressed early in the melanin biosynthesis process at a concentration of 10 ppm or more was higher than that of arbutin Respectively.

Measurement of inhibitory effect of tyrosinase activity

The effect of corn mustache extract on tyrosinase activity was measured. 120 μl of a substrate, 8.0 mM L-DOPA (dissolved in 67 mM phosphate buffer, pH 6.8), and 1 ppm, 10 ppm and 100 ppm concentrations of the test sample dissolved in 40 μl of methanol were added to a 96-well microplate and mixed . After mixing, 40 μl of tyrosinase was added, and the amount of dopachrome produced after 20 minutes was measured at the absorbance at 492 nm. Based on the measurement results, the degree of inhibition was calculated based on the following equation, and the results are shown in FIG.

* Inhibition = (control OD492 - sample OD492) / control OD492

As shown in Fig. 3, the corn mustache extract did not show significant tyrosinase activity inhibition at 100 ppm or less. In view of this, the corn mustache extract does not directly inhibit tyrosinase activity, but appears to inhibit melanin production by decreasing the amount of tyrosinase expression in melanin-producing cells.

Human skin Scarlet  Evaluation of mitigation effects on deposition

Forty - two female subjects aged 36 to 50 years (mean 42.9 ± 3.9 years) with hyperpigmented lesions on the face were examined. After dividing the subjects into two groups, a 0.75% aqueous solution of corn mustache extract and a 1.5% aqueous solution were applied to the entire face after cleansing twice a day (morning and evening) for 8 weeks.

Skin color evaluation was carried out at 4 and 8 weeks after use. After waiting for 20 minutes at constant temperature and humidity (22 ± 2 ℃, 50 ± 5%) after skin cleansing, visual evaluation, skin color measurement, Density analysis.

At each time point, the visual evaluation was independently evaluated by two testers based on the standard image with the degree of hyperpigmentation of the facial aspect as a 10-point scale. The L * value and the ITA value (ITA ° = [Arc Tangent ((L * -50) / b *)] 180 / 3.14159) were measured using a spectrophotometer (CM-2500d, manufactured by Minolta Co., . In addition, the area and density of the hyperpigmented areas were measured using a facial imaging apparatus VISIA ver. 5 (Canfield, USA), and the images were imaged using Image-pro plus (manufactured by MediaCybernetics, Inc., USA) .

As shown in Table 3 below, the visual evaluation of the hyperpigmentation sites showed that in the case of the 0.75% aqueous solution of corn beard extract, the 1.5% aqueous solution of corn mustache extract was observed at 8 weeks, The skin color tended to be improved.

Concentration (%) Elapsed Time (Week) Number of subjects (persons) Average ¹ SEM SD p-value Decrease (%)
0.75
0 21 5.79 0.33 1.52 - - 4 21 5.79 0.33 1.52 1.000 ^w 0.00 8 21 5.76 0.32 1.48 1.310 ^w 0.41
1.5
0 21 5.05 0.21 0.96 - - 4 21 5.02 0.21 0.95 0.634 ^w 0.47 ▼ 8 21 4.98 0.21 0.98 0.360 ^w 1.42 ▼ A decrease in the mean value indicates an improvement in hyperpigmentation (▼)
^W Wilcoxon sign rank test / post-hoc test

As can be seen from FIGS. 4 and 5, in the analysis of the skin color using the spectrophotometer, the values of L * value and ITA ° in the 0.75% aqueous solution and 1.5% of the corn mustard extract were compared at 4 weeks and 8 weeks after application ( P < 0.05).

In addition, a skin color image of the subject was taken for the analysis of the area and density of the hyperpigmented lesion site, and a representative color image of the subject after 8 weeks of using a 1.5% aqueous solution of corn mustache extract is shown in FIG.

The area and density of hyperpigmentation sites were analyzed using image analysis program. As a result, as shown in FIG. 7 and FIG. 8, in the case of the 0.75% aqueous solution of corn musth extract, the area of the hyperchromic spot was 8 weeks after application and the density of the hyperchromic spot was 4 weeks and 8 And significantly decreased at the end of the week. In the 1.5% aqueous solution of corn mustache extract, both area and density of hyperpigmented sites were significantly decreased at 4 weeks and 8 weeks.

Evaluation of human skin safety

The safety evaluation was conducted at 4 and 8 weeks after application of the corn beard extract, and subjective irritation and objective irritation were evaluated by the interviews and the tester's observation. There were 21 subjects.

As a result, as shown in Table 4 below, skin adverse reactions and irritation were not observed in all the subjects for 8 weeks after application of the aqueous solution of corn beard extract.

evaluation
Symptom
Corn beard extract 1.5%
4 weeks 8 weeks



Subjective irritability



itch 0 0 It stings like a needle. 0 0 It's hot. 0 0 I'm stingy. 0 0 Stiff 0 0 Pull 0 0 I have snow. 0 0 I have tears. 0 0 Etc 0 0

Objective stimulation

Erythema 0 0 edema 0 0 Construction 0 0 papule 0 0 Etc 0 0

Claims (12)

A cosmetic composition for skin whitening which inhibits melanin production by decreasing the expression amount of tyrosinase, which comprises corn hydrolyzate water extract as an active ingredient. The method according to claim 1,
Corn hydrolyzate extracts were selected from the group consisting of Ginseng 4, Gushen, Ariruk, Bokjeongju, Ulluchul 1, Ilmyul, Cham 4, Dapyeong, Cheongdaok, Pyunganok, Kangdaok, Wherein the cosmetic composition is at least one selected from the group consisting of corn starch, 3. The method according to claim 1 or 2,
A cosmetic composition for skin whitening characterized in that cornstarch water extract is contained in an amount of 0.001% to 20% based on the total weight of the composition. delete 3. The method according to claim 1 or 2,
A cosmetic composition for skin whitening characterized by further comprising at least one additive which is acceptable for cosmetics. 3. The method according to claim 1 or 2,
Wherein the composition is any one selected from the group consisting of skin, lotion, cream, gel, essence, foundation, lotion, patch, pack, soap, cleanser and detergent. A food composition for skin whitening which inhibits melanin production by decreasing the expression amount of tyrosinase, which contains corn steep liquor extract. 8. The method of claim 7,
Corn hydrolyzate extracts were selected from the group consisting of Ginseng 4, Gushen, Ariruk, Bokjeongju, Ulluchul 1, Ilmyul, Cham 4, Dapyeong, Cheongdaok, Pyunganok, Kangdaok, &Lt; / RTI &gt; wherein the composition is a whiskey water extract of at least one kind of corn selected from the group consisting of: 9. The method according to claim 7 or 8,
A food composition for skin whitening characterized by containing cornstarch water extract in an amount of 0.001% to 20% based on the total weight of the composition. delete 9. The method according to claim 7 or 8,
A food composition for skin whitening characterized by further comprising at least one additive which is acceptable for food. 9. The method according to claim 7 or 8,
Wherein the composition is in any one form selected from the group consisting of beverage, gum, tea, confectionery, vitamin complex, and health supplement.

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