KR101888999B1 - Cosmetic composition containing ultrasonicating extract of mahkota dewa - Google Patents

Abstract

The present invention relates to a process for the preparation of More particularly, the present invention relates to a cosmetic composition containing an ultrasound extract of Macotamide and an extract of fermented black yeast thereof to improve skin moisturizing effect, skin barrier improvement effect, and skin protection with improved antioxidant ability To a cosmetic composition.

Description

COSMETIC COMPOSITION CONTAINING ULTRASONICATING EXTRACT OF MAHKOTA DEWA [0002]

The present invention relates to a cosmetic composition containing macoradade and an ultrasonic extract, wherein the cosmetic composition according to the present invention is excellent in skin moisturizing function and antioxidative activity function.

In 2003, the major causes of death in Korea were cancer, cerebrovascular disease, cardiovascular disease, and diabetes in descending order (Korea National Statistical Office 2003. Annual Report of the Cause of Death Statistics. ). It is known that reactive oxygen species (ROS) can be a major cause of chronic degenerative diseases such as cardiovascular disease, diabetes, cancer, degenerative neuropathy, and aging, and antioxidants can help prevent chronic degenerative diseases (Bray, TM Nutrition. 16, pp. 588-581, 2000).

Since all aerobic organisms involve inevitably harmful reactive oxygen species during metabolism, all organisms must have the mechanism to remove these reactive oxygen species before they can survive. Active oxygen species can be broadly categorized into two types, oxygen radical species and compounds that cause oxygen radical species to form, such as hydrogen peroxide. As already known, hydrogen peroxide is converted to oxygen or water by catalase or peroxidase, but in the case of oxygen radical species elimination mechanism, oxygen radicals other than the mechanism of eliminating O ₂ ^- (superoxide anion) The species removal mechanism has not yet been clarified. Hydroxy radicals, a type of oxygen radical species, are formed by the reaction of H ₂ O ₂ and O ₂ in living organisms and are known to exist in almost all biomolecules (Fridovich et al., Ann. Biochem., 44, pp147-159, 1975; Tauber et al., Blood, 53, pp666-676, 1979).

Therefore, it is clear that in order to maintain homeostasis in vivo, it is inevitably required to protect against hydroxy radicals which are highly reactive. In this respect, organic materials such as ascorbic acid and uric acid are known to remove hydroxy radicals through chemical reactions, but this is not a radical removal of hydroxy radicals, but a conversion into radicals with low reactivity, (Borg, DC and Schaich, KM, Handbook of Free Radicals and Antioxidants in Biomedicine, Vol. I, pp. 63-75) 80, CRC press, 1989).

The active radicals (O ₂ ^- , H ₂ O ₂ , OH ^-, and O ₂ ) generated during the human physiological process are highly reactive, and the free radical reactions caused by them cause destruction of major macromolecules including lipids Davies, KJA and Goldberg, AL, J. Biol. Chem., 262, p8220-8226, 1987). They are increased in their production by various factors that lead to normal metabolic processes, photosensitization reactions, drug metabolism and other abnormalities of cell metabolism. Therefore, living organisms are always exposed to the harmful effects of free radical reactions caused by them (Morehouse, LA and Thomas, CE, J. Free Radicals Biol. Med., 1, p8, 1985). Particularly, as the age of the cells increases, harmful effects of these harmful radicals are continuously accumulated, leading to aging of the cells as well as diseases related to various adult diseases due to an increase in age such as cancer, atherosclerosis, heart disease and skin aging (Harman, D., Free Radicals in Biology, Academic Press, New York, pp. 255-275, 1982).

Therefore, it is desired to find candidates that are expected to be useful for the treatment of diseases such as lipid metabolism, immune diseases and cancer by inhibiting the free radical reaction in the body by reactive oxygen metabolites or by removing harmful active oxygen accumulated in the human body Have continued.

 The present inventors have studied the applicability of various natural products to cosmetics. As a result, it has been found that the effect of skin moisturizing effect, skin soothing effect, and soothing action on macorhadade and ultrasound extract, and macoradaceae fermented with black yeast mycelium, , Antioxidative effect, skin barrier improvement effect, and completed the present invention.

Therefore, the inventors of the present invention are based on the surprising result that the skin moisturizing effect is greatly improved when the extract of Dekotadeya is prepared using ultrasonic extraction instead of the commonly used methods such as hot water extraction, room temperature extraction and warm-up extraction. Accordingly, it is an object of the present invention to provide a cosmetic composition comprising macrolide and an ultrasound extract as an active ingredient. In addition, the present invention relates to a natural extract obtained by fermenting Makorotade and an ultrasonic extract with mycelia of black yeast, and a skin extract containing the same as an active ingredient. The skin moisturizing effect (Experimental Example 1), skin barrier improving effect (Experimental Example 2) 3).

The present invention relates to a cosmetic composition comprising macrolide and an ultrasound extract as an active ingredient.

Preferably, the macoradene and the ultrasonic extract are prepared by mixing macoradade with purified water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, Is added to an extraction solvent selected from the group consisting of methanol, chloroform, ethyl ether, butylene glycol, hexane, and mixtures thereof, followed by extraction with an ultrasonic extractor for about 1 to 12 hours.

Preferably, the macrotade and the ultrasonic extract comprise macrotade and the ultrasonic extract in combination with Aureobasidium pullulans ) and black yeast fermentation macaquatade and ultrasonic extracts obtained by fermentation.

Preferably, the black yeast fermentation macrotide and the ultrasonic extract are obtained by fermenting macortade and an ultrasonic extract with black yeast for 1 to 7 days while maintaining a pH of 5 to 7 under a temperature condition of 20 to 40 ° C .

Preferably, the cosmetic composition has a skin barrier function recovery effect.

Preferably, the cosmetic composition has an antioxidative effect.

Preferably, the cosmetic composition has skin moisturizing effect.

Preferably, the macortase and the ultrasonic wave extract are contained in an amount of 0.0001 to 50.0% by weight based on the total weight of the cosmetic composition.

Preferably, the cosmetic composition is a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, cleansing oil, powder foundation, emulsion foundation, wax foundation, pack, massage cream and spray ≪ / RTI >

The present invention provides a cosmetic composition comprising macrotadine, an ultrasound extract of Makotade, which is applicable to skin cosmetics and which is harmless to human body and is highly safe, and an ultrasound extract of black yeast fermented Macotade and an effective ingredient .

 The ultrasonic wave extract of Mahkota dewa according to the present invention shows a moisturizing effect (Experimental Example 1), a skin barrier improving effect (Experimental Example 2), and an antioxidative ability (Experimental Example 3) by DPPH. The cosmetic composition containing the black yeast fermented macrotide and the ultrasonic wave extract of the present invention is superior in skin moisturizing effect to the existing cosmetic and extract cosmetic composition.

In addition, the free radical scavenging activity of macrotade and ultrasonic extract according to the present invention was measured, and it showed antioxidative activity similar to that of L-ascorbic acid, which is a standard substance. In addition, Macorodia is a medicinal substance which has been used for a long time as an edible or herbal medicine, and thus the extracts of the present invention have no problems such as toxicity and side effects.

Unless defined otherwise, all technical terms used in the present invention have the following definitions and are consistent with the meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Also, preferred methods or samples are described in this specification, but similar or equivalent ones are also included in the scope of the present invention.

 The term "about" is used herein to refer to a reference quantity, a level, a value, a number, a frequency, a percent, a dimension, a size, a quantity, a weight, or a length of 30, 25, 20, 25, 10, 9, 8, 7, Level, value, number, frequency, percent, dimension, size, quantity, weight or length of a variable, such as 4, 3, 2 or 1%.

 Throughout this specification, the words "comprises" and "comprising ", unless the context requires otherwise, include the steps or components, or groups of steps or elements, Steps, or groups of elements are not excluded.

 In order to achieve the above object, the present invention provides a skin moisturizing and antioxidant composition comprising, as an active ingredient, an extract of Mahkota dewa having remarkable skin moisturizing and antioxidative effects.

The inventive Mahkota dewa ) is an arthropod plant that lives in Indonesia. It is used for pain treatment and medicines, and it is known to be effective for itching and cancer treatment. Especially good for women as a good sleeping effect, and flavonoids, alkaloids, saponin, bananin, is a major component of the tefenoid.

Macotade used in the present invention means a product obtained by extracting from a variety of organs such as the outpost, leaves, stems, roots, flowers, and fruits of Macotade with an ordinary method, and preferably refers to an extract obtained from a fruit do.

In the present invention, "ultrasonic extraction" is a method in which macorotadecas are dissolved in water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, glycerin, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, Means extraction with an extraction solvent using at least one solvent selected from the group consisting of methanol, chloroform, ethyl ether, butylene glycol, hexane and a mixture thereof, followed by extraction with an ultrasonic extractor for about 1 to 12 hours. The time of the ultrasonic extraction may be varied depending on the amount of the sample to be extracted, and the result of the extraction may be filtered or purified.

In addition, the macortase and the ultrasonic extract of the present invention may be produced in powder form by an additional process such as vacuum distillation and freeze-drying or spray-drying.

The present invention can include black yeast fermentation macaquatade and ultrasonic extract. Black yeast fermentation The yeast extract and the ultrasonic wave extract can be obtained by fermenting the extracts of Makotade and the ultrasonic wave with 1 to 7 days while maintaining the pH at 5 to 7 under the temperature condition of 20 to 40 ° C as black yeast.

Specifically, it is as follows. First, the saccharide can be sterilized by adding 1 to 5% by weight of glucose as a carbon source and 0.1 to 1% by weight, and preferably 0.2 to 0.5% by weight of peptone to the extracts of macrotides and ultrasonics. It can be cultured by inoculating a culture medium of Aureobasidium pullulans strain so as to have a concentration of 25 g / L. The culture can be carried out using a 5 L fermenter for 1 to 7 days at 20 to 40 ° C while maintaining the pH at 5-7. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture. After the fermentation, the fermentation product of the fermentation product with the first step of removing the cultured bacteria is added with ethanol so as to be a final 70% (v / v) ethanol solution, refluxed, cooled, and filtered. Upon completion of the filtration, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 DEG C or lower to produce the black yeast fermentation macrotide and the ultrasonic extract of the present invention.

The macotamide according to the present invention and the ultrasonic extract or the black yeast fermented macrotadine and the ultrasonic extract may be contained in an amount of 0.0001 to 50.0% by weight, preferably 0.001 to 30.0% by weight, based on the total weight of the cosmetic composition. If the content is less than 0.0001% by weight, the desired effect can not be obtained. If the content is more than 50.0% by weight, the effect of increasing the content is insignificant and not economical.

The cosmetic composition according to the present invention may comprise a cosmetically acceptable base. For example, the cosmetic of the present invention may further comprise a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymeric polysaccharides, sphingolipids and seaweed extracts. In addition, the components contained in the cosmetic composition of the present invention may contain, as an active ingredient, components commonly used in cosmetic compositions in addition to the above extract or compound, for example, stabilizers, solubilizers, vitamins, And may contain conventional customary auxiliaries and carriers.

The cosmetic composition of the present invention can be prepared into any of the formulations conventionally produced in the art and can be used in the form of solutions, suspensions, emulsions, pastes, gels, creams, lotions, powders, soaps, , Oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto.

More specifically, it can be manufactured in the form of a soft lotion, a nutritional lotion, a nutritional cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray or a powder.

When the formulation of the present invention is a paste, cream or gel, an animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as the carrier component .

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component. In the case of a spray, in particular, / Propane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, , 3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid esters.

In the case where the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

When the formulation of the present invention is an interfacial active agent-containing cleansing, the carrier component is selected from aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing agent, or a surfactant-free cleansing agent, it may be applied to the skin and then wiped off or removed or washed with water. The surfactant-containing cleansing formulation is a cleansing foam, a cleansing water, a cleansing towel, and a cleansing pack. The surfactant-free cleansing formulation may be a cleansing cream, , Cleansing lotion, cleansing water and cleansing gel, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Production Examples. These production examples are only for explaining the present invention in more detail and that the scope of the present invention is not limited by these production examples according to the gist of the present invention, It will be obvious.

[ Comparative Manufacturing Example  One] Makoto  Preparation of solvent and water extracts

The macortamide was freeze-dried and ground, and then purified water corresponding to 10 times the weight of the sample was added to the sample and extracted with a magnetic stirrer for 12 hours. The extract and the residue were separated by filtration under reduced pressure. Then, the obtained residue was added with Makotade at the start of extraction and purified water corresponding to 5 times its weight, and the mixture was extracted with a magnetic stirrer for 6 hours. After filtration under reduced pressure, purified water corresponding to one part by weight of macortamide at the start of extraction was added to the obtained residue, and the mixture was refluxed three times for 5 hours. After cooling, filtration was carried out using Whatman # 3 filter paper Thereafter, the mixture was concentrated under reduced pressure and freeze-dried at 50 ° C or lower. The lyophilized product was dissolved in 50% glycerin to 2% (w / v) to prepare macoradade extract.

[ Comparative Manufacturing Example  2] Makoto  Preparation of Dew and Solvent (Ethanol) Extracts

 In Comparative Preparation Example 1, the extraction solvent was replaced with ethanol, and macoradate and ethanol extracts were obtained through a process similar to the production process of Comparative Preparation Example 1.

[ Manufacturing example  One] Makoto  Manufacture of dewatering ultrasonic extracts

 Macotamide was prepared by using an ultrasonic extractor. Macotamide was selected as a sample, washed thoroughly, and finely pulverized to pass through a 300-mesh sieve. 70% ethanol was added thereto so as to make 100 g / L of the macadamycin and the pulverized product, and the mixture was extracted with an ultrasonic wave extractor (natural solution) at room temperature for 4 hours, cooled, filtered through whatman # 3 filter paper, And concentrated under reduced pressure and lyophilized. The lyophilized product was dissolved in 2% (w / v) of 50% glycerin to obtain macrotadine and an ultrasonic extract.

[ Manufacturing example  2] Black yeast  Fermentation Makoto  Manufacture of dewatering ultrasonic extracts

Black yeast fermentation The yeast extract and the ultrasonic extract were prepared by fermenting the above-mentioned Makotade and the ultrasonic wave extract (Preparation Example 1) with black yeast. First, 3% by weight of glucose as a carbon source and 0.5% by weight of peptone are added to macoradate and ultrasonic extract to sterilize. It can be cultured by inoculating a culture medium of Aureobasidium pullulans strain so as to have a concentration of 25 g / L. The culture can be carried out using a 5 L fermenter for 7 days at 37 ° C and at a pH of 7. After culturing, the culture broth was centrifuged and the culture was firstly removed and sterilized (121 ° C, 15 minutes, 1.5 atm) to prevent further culture.

 After the fermentation, the fermentation product of the fermentation product with the first step of removing the cultured bacteria is added with ethanol so as to be a final 70% (v / v) ethanol solution, refluxed, cooled, and filtered. Upon completion of the filtration, the extract obtained in the extracting step is transferred to a concentration tank and concentrated under reduced pressure or freeze-dried at 50 DEG C or lower to produce the black yeast fermentation macrotide and the ultrasonic extract of the present invention.

[ Formulation Example  And Comparative Formulation Example  Produce]

As shown in the following Table 1, Formulation Examples 1 and 2 were prepared as cosmetics containing macoradade and ultrasonic wave extract (Preparation Example 1) and black yeast fermentation macrotade and ultrasonic wave extract (Preparation Example 2) (Comparative Preparative Examples 1 and 2) and Comparative Formulations 1 to 3 as a cosmetic composition containing glycerin and 1,3-butylene glycol as a moisturizing agent instead of macoradade extract, Comparative Formulation Example 4 was prepared as a cosmetic composition containing no moisturizer.

(Unit: wt%) ingredient Formulation Example 1 Formulation Example 2 Comparative Formulation Example 1 Comparative Formulation Example 2 Comparative Formulation Example 3 Comparative Formulation Example 4 Production Example 1 5.0 - - - - - Production Example 2 - 5.0 - - - - Comparative Preparation Example 1 - - 5.0 - - - Comparative Production Example 2 - - - 5.0 - - glycerin - - - - 5.0 - 1,3-butylene glycol - - - - 5.0 - EDTA-2Na 0.02 0.02 0.02 0.02 0.02 0.02 Cetostearyl alcohol 2.0 2.0 2.0 2.0 2.0 2.0 Glyceryl stearate 1.5 1.5 1.5 1.5 1.5 1.5 Microcrystalline 0.5 0.5 0.5 0.5 0.5 0.5 Squalane 5.0 5.0 5.0 5.0 5.0 5.0 Liquid paraffin 2.0 2.0 2.0 2.0 2.0 2.0 Trioctanoin 3.0 3.0 3.0 3.0 3.0 3.0 Polysorbate 1.0 1.0 1.0 1.0 1.0 1.0 Sorbitan stearate 0.5 0.5 0.5 0.5 0.5 0.5 Tocopheryl acetate 0.1 0.1 0.1 0.1 0.1 0.1 Cyclomethicone 4.0 4.0 4.0 4.0 4.0 4.0 BHT 0.05 0.05 0.05 0.05 0.05 0.05 Incense, preservative Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Suitable amount Purified water to 100 to 100 to 100 to 100 to 100 to 100

[ Experimental Example  1] Moisturizing effect

The clinical moisturizing effect and the sustaining ability of the cosmetic compositions of Formulation Examples 1 and 2 and Comparative Formulation Examples 1 to 4 were measured as follows. A questionnaire survey was conducted on 120 healthy adult men and women who were randomly divided into 20 groups. Two formulations and two comparative formulations for each group were administered to each group. Respectively. Moisturizing effect was evaluated by quantifying the increase rate (%) of skin conductivity according to the skin moisture content using the Corneometer CM 820 (Corage + Khazaka, Germany) for 2 weeks from the start of the test and after the completion of the test. The experimental results are shown in Table 2 below.

Increase rate of skin electrical conductivity (%) division Before use After 2 weeks of use After 4 weeks of use Formulation Example 1 24.79 54.87 63.54 Formulation Example 2 25.83 60.12 85.82 Comparative Formulation Example 1 25.24 41.51 48.24 Comparative Formulation Example 2 25.58 42.10 49.65 Comparative Formulation Example 3 26.72 37.18 41.88 Comparative Formulation Example 4 24.12 26.74 27.52

As can be seen from the above Table 2, Formulation Example 1 containing macrotade and ultrasonic wave extract according to the present invention and Formulation Example 2 containing black yeast fermented ultrasonic wave extract had comparative formulations containing macrolide and solvent extract It can be seen that the dermal electrical conductivity increase rate is superior to that of Comparative Formulation Example 3 containing Examples 1 and 2 and chemical moisturizing agent. In general, the skin conductivity is proportional to the amount of skin moisture. Therefore, the above results show that the cosmetic composition containing macoradade, the ultrasound extract, the black yeast fermentation macortade and the ultrasonic extract according to the present invention has a higher skin moisture content It is confirmed that it maintains. In addition, skin irritation could not be observed in all of the subjects who applied the cosmetic composition to the skin.

[ Experimental Example  2] Skin barrier  Improvement effect

25 ℃, relative humidity of 45% and 25 healthy women in the room without air flow. Skin barrier damage was induced using a 1% aqueous solution of sodium lauryl sulfate (SLS). After applying the cosmetic compositions of Formulations 1 and 2 and Comparative Formulations 1 and 2 to the upper arm of the arm, TEWAMETER TM210 (C + K eletronic GmHH, Germany). That is, TEWL (Transepidermal Water Loss) value was measured by changing the TEWL value with time, and skin barrier recovery rate (%) was confirmed. The experimental results are shown in Table 3 below.

Skin barrier improvement effect division Skin barrier recovery rate (%) 2D 4D 6D 8D Formulation Example 1 28.17 47.35 65.37 79.09 Formulation Example 2 51.17 67.35 85.37 99.09 Comparative Formulation Example 1 21.16 36.39 57.12 68.35 Comparative Formulation Example 2 22.31 38.59 56.74 69.52

As a result of measuring the skin barrier improvement effect as shown in Table 3, it was found that Formulation Example 2 containing the cosmetic material according to the present invention, particularly black yeast fermentation macrotadine and ultrasonic wave extract, had better transdermal water loss Was greatly improved.

[ Experimental Example  3] In vitro In the DPPH method  Antioxidant activity assay

The DPPH method (Blois, MS: Antioxidant determination by the use of a stable free radical, Nature, 26, 1199-1200, 1958) was used to measure the in vitro antioxidative effect of the extracts of macrotides. The DPPH (1,1-diphenylpicrylhydrazyl) method is a method of producing a bioactive agent exhibiting antioxidative activity such as tocopherol, ascorbate, flavonoid compound, aromatic amine, Maillard type browning agent, L-ascorbic acid was used as a standard product to measure the antioxidative effect by the hydrogen donating ability according to the degree of discoloration of the deep purple color by reduction by the substance, and 2 x 10 ^-4 M DPPH was added to each sample, and the absorbance at 517 nm Were measured.

Samples and standards DPPH free machine 0.01 g / L 0.03 g / L 0.05 g / L Comparative Preparation Example 1 21.6% 47.4% 78.7% Comparative Production Example 2 24.9% 52.6% 81.9% Production Example 1 30.7% 61.9% 87.8% Production Example 2 35.1% 73.6% 95.1% L-ascorbic acid 25.4% 55.9% 83.6%

As a result, the DPPH free radical scavenging activities of Comparative Preparation Examples 1 and 2 and Preparation Examples 1 and 2 were 78.7%, 81.9%, 87.8%, and 95.1% at the concentration of 0.05 g / L, respectively, and L- The activity of acid was 83.6%. In other words, the macotadeles and extracts of Preparation Examples 1 and 2 exhibited high activity comparable to other antioxidants, and in particular, Production Example 2 exhibited the highest activity. In particular, it was found that the extracts of Makotade and Ultrasound (black yeast fermentation) showed significant antioxidant activity despite being a mixture of natural products, and the effect of antioxidant activity was improved by black yeast fermentation.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the same is by way of illustration and example only and is not to be construed as limiting the scope of the present invention. It is therefore intended that the scope of the invention be defined by the claims appended hereto and their equivalents.

Claims (8)

A cosmetic composition comprising macoradate and an ultrasonic extract as an active ingredient and having a skin barrier function recovery effect or skin moisturizing effect. The method according to claim 1,
The above-mentioned macrotide and ultrasonic extract can be prepared by mixing macoradate with purified water, anhydrous or lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate and 1,3-butylene glycol, propylene glycol, dichloromethane, Is added to an extraction solvent selected from the group consisting of ethyl ether, butylene glycol, hexane, and mixtures thereof, followed by extraction with an ultrasonic extraction device for 1 to 12 hours. The method according to claim 1,
Wherein the macrotide and the ultrasonic extract are a black yeast fermented macrotide and an ultrasonic extract obtained by further fermenting macoradate and an ultrasonic extract with Aureobasidium pullulans . The method of claim 3,
Wherein the black yeast fermentation macrotide and the ultrasonic extract are obtained by fermenting macortade and an ultrasonic extract with black yeast for 1 to 7 days while maintaining the pH at 5 to 7 under a temperature condition of 20 to 40 캜 . 5. The method according to any one of claims 1 to 4,
Wherein the macotamide and the ultrasonic wave extract are contained in an amount of 0.0001 to 50.0% by weight based on the total weight of the cosmetic composition. delete delete delete