KR101772824B1 - Cattle feed for improving flesh, breeding method using it, and beef having high oleic acid - Google Patents

Abstract

The present invention relates to a cattle feed composition for improving meat quality. The cattle feed composition includes: roughage substances; enriched feed substances including energy complexes; and additives including phyllite, limestone, sulfur, vitamins, salt, and probiotics mixed therein. The cattle feed composition uses the relatively low-cost roughage substances and effective probiotics and energy complexes in order to significantly increase the content of an oleic acid in beef. The present invention uses the composition suitable for periods before and after growth completion to grow cattle, thereby increasing the content of the oleic acid, rough saponin, and sulfur, and unsaturated fatty acid in beef.

Description

TECHNICAL FIELD The present invention relates to a cattle feed composition for improving meat quality, a cattle breeding method using the cattle feed composition, and a cattle feed having high oleic acid content,

The present invention relates to a cattle feed composition for improving the meat quality of cattle, in particular, Korean beef cattle, a method of breeding cattle using the cattle feed, and a beef obtained from cattle fed with the same. More specifically, the present invention relates to a cattle feed composition which contains high levels of oleic acid, The present invention relates to a cattle feed composition for obtaining beef having a high content of unsaturated fatty acids, a method of breeding cattle using the cattle feed composition, and a beef having a high content of oleic acid obtained from the fed cattle.

The cow is a ruminant, with a large first place to contain food to eat, a second to have a honeycomb-like wall, a third to have a mucosal wrinkle, and a fourth to have a fourth It has a rumen. Feeds fed by animals with rumen are mixed by the peristalsis during the stay on the first stage and digested by the action of various microorganisms or enzymes in the stomach. If the fiber content is low in the whole feed, And an abrupt pH drop occurs. If the pH of the rumen is below 6, starch is not digested well, so the ingested starch can not be used in the rumen and fermented in the small intestine. Therefore, it is essential to feed the cow to the cow when feeding the cow.

In order to improve the availability of concentrated feed to ruminants, the use of forage must be used. However, since the feed itself is low in nutrient content, the total energy concentration decreases as the ratio of feed to feed increases. On the other hand, when the amount of the forage is too low, the amount of nutrients in the feed increases, but the incidence of metabolic diseases increases and the daily gain decreases.

Oleic acid, a type of monounsaturated fatty acid, is a factor that affects the taste of meat. It weakens the degree of toughness of the meat, makes it feel soft when chewing meat, When cooked, oleic acid melts quickly to form a film on the surface of the meat, thereby suppressing the evaporation of water, and at the same time, reducing the production of moisture by denaturation of the meat protein and improving the juiciness. Therefore, when the content of oleic acid contained in the beef is high, the palatability is high, and when the content of oleic acid is relatively low, the palatability is decreased.

Oleic acid is also known as lactic acid, and it is contained in plant and animal species. Especially, it is main component of oils such as camellia oil and olive oil, and exists in more than 80% in constituent fatty acids such as olive oil and camellia oil. Oleic acid is a monounsaturated fatty acid, which can not be oxidized compared to other fatty acids. In addition, oleic acid has the effect of lowering the cholesterol level by lowering the content of low density protein cholesterol known as blood cholesterol, so that ingesting oleic acid helps maintain blood vessels clean and maintain blood circulation.

Smith, a professor at Texas A & M University, conducted a survey of 27 normal males in the United States by eating 114 g of burgers per week for 5 weeks with meat containing a large amount of unsaturated fat (oleic acid) and less meat, (HDL) levels in the blood of people who consume burgers that contain high levels of fat (oleic acid) are six times higher. As a result of these experiments, it is effective to prevent cardiovascular diseases by eating meat containing a lot of unsaturated fats (oleic acid).

According to the current grade standards, the grade of beef is divided into meat quality grade and meat grade. The meat quality grade is a criterion for the consumer to select the meat by judging the meat quality as 1 ++ class, 1+ class, 1 class, 2 class and 3 class depending on the degree of fatness, color, color, texture and maturity. According to the weight index obtained by measuring the weight of the conductor, the thickness of the backfill and the cross sectional area of the root of the carcass, the carcass grade obtained from the carcass is classified into Class A (meat weight index 67.20 or more), Class B (meat weight index 63.30 to less than 67.20) ), And C (less than 63.30). The relationship between meat quality grade and meat grade is shown in the following table.

According to the current classification standards, the higher the grade (1 ++), the higher the saturated fatty acid content, which is harmful to health than the unsaturated fatty acid, and the longer the fat content to increase the grade (1 ++) The amount of meat) is reduced, which is an uneconomical problem.

Therefore, it is required to develop a feed composition capable of improving the quality of beef while optimizing the growth of cattle.

Korean Patent Registration No. 10-1320048 "Cow feed composition for improvement of meat quality and method of cow breeding using the same, and saffron having high content of saponin, sulfur and unsaturated fatty acid obtained from cows fed the feed," The content of oleic acid and amino acid is higher than that of general Korean beef by containing saponin and sulfur, high unsaturated fatty acid, and having higher contents of oleic acid and amino acid. The present invention provides a cattle feed composition and a breeding method capable of obtaining high quality beef. However, in this method, it is possible to obtain consistent results in the case of saponin and sulfur, but in oleic acid, it is generally higher than that in general Hanwoo, but it is difficult to obtain beef having high content of oleic acid consistently because of variation in contents depending on individuals.

Korean Patent Registration No. 10-1320048 Korean Patent Registration No. 10-0775134

The present invention provides a cattle feed composition and a breeding method which can consistently increase the content of oleic acid and thus obtain a beef having a high content of oleic acid and an oleic acid content comparable to or higher than that of Japanese fowl, .

It is another object of the present invention to provide a cattle feed composition and a breeding method which can obtain an excellent quality beef having a high content of oleic acid using an effective energy complex and a probiotic agent while lowering the production cost by using a relatively inexpensive forage.

The present invention also aims at a probiotic for cow breeding containing a variety of useful microorganisms by a natural fermentation method and a solid probiotic composition capable of effectively administering it.

It is another object of the present invention to provide a high quality beef having high oleic acid content and high crude saponin and sulfur content and high unsaturated fatty acid content by the above composition and method.

In order to achieve the above object,

15 to 25 parts by weight of a feedstock for at least one selected from the group consisting of Sudan grass, Holly rice, rice straw and oat hay; 50 to 70 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of the first additive,

For the latter, 10 to 20 parts by weight of a forage, which is at least one selected from Sudan grass, Holli rice, rice straw and oat hay; 60 to 80 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of a second additive,

The first additive is contained in a ratio of 10 to 20 parts by weight of phyllite, 6 to 10 parts by weight of limestone, 1 to 4 parts by weight of sulfur, 1 to 5 parts by weight of vitamin, 1 to 5 parts by weight of salt, and 65 to 90 parts by weight of a probiotic Lt; / RTI >

The second additive is a mixture comprising 11 to 21 parts by weight of phyllite, 7 to 11 parts by weight of limestone, 1 to 6 parts by weight of sulfur, 1 to 5 parts by weight of salt, and 70 to 95 parts by weight of a probiotic agent,

The prophylactic agent is 8 to 13 parts by weight of pineapple; 8 to 13 parts by weight of rice straw; 8-13 parts by weight of phyllite; 20 to 42 parts by weight of a herbal medicine containing bellflower, Doduck, 20 to 42 parts by weight of corn meal; 8 to 13 parts by weight of vitamin B by-products are cultured at 30 to 35 DEG C for 2 to 5 days, and then the cultured raw materials are mixed and fermented and aged at 28 to 32 DEG C for 5 to 10 days. Thereby providing a cattle feed composition for improving meat quality.

In the cow feed composition, the concentrated feed is preferably 15 to 25 parts by weight of palm or ground corn, 28 to 38 parts by weight of embryo, 39 to 49 parts by weight of energy complex, 31 to 41 parts by weight of bread, 85 to 95 pineapple By weight and 29 to 39 parts by weight.

In the cow feed composition, the first additive and the second additive are preferably a solid obtained by mixing the celestial rock, limestone, sulfur, vitamin, salt, and probiotics in a fermentation storage at 20 to 35 ° C for 5 days or more It is a probiotic agent.

In the cow feed composition, the probiotic agent is preferably selected from the group consisting of Bacillus subtilis, Bacillus ricinifomis, Bacillus sonolenisis, Bacillus coagulans, Bacillus amyloliquequisaceans, Pediococcus pentosaceus, And more than five kinds of microorganisms, for example, Setherabiasis, Tetrathiobacter cicamylensis, Streptomyces aureotus and Fanny Bacillus cookies.

In the cow feed composition, the energy complex preferably contains 18 to 22 parts by weight of rice bran, 23 to 27 parts by weight of olive, 13 to 17 parts by weight of walnut, 8 to 12 parts by weight of prune, 6 to 7 2 to 4 parts by weight of an isotonic soybean, and 18 to 22 parts by weight of an almond hull.

In the cow feed composition, the forage is composed of rice straw or rice straw and orchard hay.

Further, according to the present invention,

15 to 25 parts by weight of a feedstock having at least one selected from the group consisting of Sudan grass, Holly rice, rice straw and oat hay; 50 to 70 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of a first additive to a poultry meat; And

10 to 20 parts by weight of a forage which is at least one selected from the group consisting of Sudan grass, Holli rice, rice straw and oat hay; 60 to 80 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of a second additive at a later stage of the frying,

The first additive is contained in a ratio of 10 to 20 parts by weight of phyllite, 6 to 10 parts by weight of limestone, 1 to 4 parts by weight of sulfur, 1 to 5 parts by weight of vitamin, 1 to 5 parts by weight of salt, and 65 to 90 parts by weight of a probiotic Lt; / RTI >

The second additive is a mixture comprising 11 to 21 parts by weight of phyllite, 7 to 11 parts by weight of limestone, 1 to 6 parts by weight of sulfur, 1 to 5 parts by weight of salt, and 70 to 95 parts by weight of a probiotic agent,

Wherein the probiotics are 8 to 13 parts by weight of pineapple; 8 to 13 parts by weight of rice straw; 8-13 parts by weight of phyllite; 20 to 42 parts by weight of a herbal medicine containing bellflower, Doduck, 20 to 42 parts by weight of corn meal; Vitamin B by-product 8-13 parts by weight Each of the raw materials is cultured at 30-35 ° C for 2-5 days, and the cultured raw materials are mixed and fermented for aging at 28-32 ° C for 5-10 days. Provide breeding methods.

In the breeding method, it is preferable to feed the feed composition for poultry meat at a feed rate of 12 to 16 kg / day in the poultry meat production and feed the feed composition for the fattening later stage at 16 to 17 kg / day.

The present invention also provides a method for producing a cow feed composition, which comprises the steps of: (a) producing 50 to 65% of oleic acid and 55 to 70% of unsaturated fatty acid in the fatty acid and 4 to 7% 0.3% (by building basis) of beef.

The cattle feed composition and the breeding method of the present invention have a higher content of oleic acid than that of general Korean beef, and a beef having an oleic acid content higher than or equal to that of Japanese beef can be obtained. Further, the present invention can obtain an excellent quality beef having high oleic acid content by using an effective biocide and an energy complex obtained by a natural fermentation method while using a relatively inexpensive forage. The beef obtained according to the present invention has high oleic acid content, high crude beef content containing crude saponin and sulfur, and high unsaturated fatty acid content.

One. Cattle feed  Composition

The cattle feed composition of the present invention comprises 15 to 25 parts by weight of a feed for at least one selected from the group consisting of Sudan grass, Holliac grass, rice straw and oat hay; 50 to 70 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of the first additive.

The cow feed composition of the present invention comprises 10 to 20 parts by weight of a forage, which is at least one selected from Sudan grass, Holli rice, rice straw and oat hay; 60 to 80 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of the second additive.

Each will be described below.

(1) Forage rates

As the roughage, at least one selected from Sudan grass, Whole ryegrass, Rice straw and Oaten hay can be used. Preferably, rice straw may be used, or rice straw and oak hay may be used together.

These forage costs are lower than those used in Korea Patent No. 10-1320048, which can reduce feed costs.

(2) Concentrated feed

The concentrated feeds contain the essential components of energy complex and pineapple, such as palm, crushed corn, barley brewed poultry, embryo, energy complex, bread, corn gluten feed, pineapple, Or more.

The concentrated feed contains 15 to 25 parts by weight of a palm or ground corn, 28 to 38 parts by weight of an embryo, 39 to 49 parts by weight of an energy complex, 31 to 41 parts by weight of bread, 85 to 95 parts by weight of pineapple and 29 to 39 parts by weight It is particularly preferable to use them in combination.

In the present invention, "energy complex" is defined as an energy feed composite comprising rice bran, olive oil, walnut oil, and sunflower seed oil. Preferably, the energy composites include 20 parts by weight of rice bran, 25 parts by weight of olive oil, 15 parts by weight of walnut oil, 10 parts by weight of prunes, 7 parts by weight of sunflower seeds, 3 parts by weight of outline soybean, and 20 parts by weight of almond hull , And preferably TDN (total amount of digestible nutrients) is about 72 to 74%.

In general, when the TDN level is the same, the concentrated feed is characterized by the lower energy loss due to the first-stage fermentation heat at the intake energy than the forage and the higher the net energy. To maintain more than 50% of 1 ++ grade beef, more than 40 mg of vitamin C should be supplied per kg of body weight. In the present invention, the pineapple may be added to the feed to provide sufficient supply of vitamin C to the cattle. Therefore, the differentiation of the fat precursor cells of the bovine ovine precursor cells into the fat cells is facilitated, the control of the fat crossing and the body fat development can be controlled, the development is promoted, and the effect of the crossing of the fat cross section and the fat crossing is effective.

(3) Additives

Additives include phyllite, limestone, sulfur, vitamins, salt and probiotics.

The first additive used in the feed composition for poultry meat and breeding comprises 10 to 20 parts by weight of phyllocarcin, 6 to 10 parts by weight of limestone, 1 to 4 parts by weight of sulfur, 1 to 5 parts by weight of vitamins, 1 to 5 parts by weight of salt, And mixed in a ratio of 65 to 90 parts by weight.

The second additive used in the feed composition for late finishing is mixed at a ratio of 11 to 21 parts by weight of phyllotaxis, 7 to 11 parts by weight of limestone, 1 to 6 parts by weight of sulfur, 1 to 5 parts by weight of salt and 70 to 95 parts by weight of a probiotic .

Preferably, the first additive and the second additive are obtained by aging the fermented rock, limestone, sulfur, vitamin, salt, and probiotics in a fermentation storage at 20 to 35 ° C for 5 days or more, . Preferably, the above-mentioned phyllotaxis, limestone, sulfur, vitamin, salt, and probiotics are put into a mixer, mixed in a mixer for 30 minutes or more, sealed with air, and then fermented for more than 5 days at 20 ° C or higher A solid probiotic agent is obtained.

① Phyllite

Phyllite is _{Fe 2 O 3 3.58%, Al} 2 O 3 11.62%, K 2 O 4.79%, Na 2 O 5.20%, SiO 2 71.20%, the silicon dioxide with CaO 1.32% and MgO 0.65% (SiO ₂₎ and aluminum oxide (Al ₂ O ₃ ) as a main component and contains ferric oxide (Fe ₂ O ₃ ), which is essential for human and living cells. It also contains rare earth elements such as lanthanum (La), neodymium (Nd), and vanadium (V). It contains more than 40 kinds of minerals. It supplies excellent natural minerals to maintain the vigor of the cells by regulating the development and physiological functions of the living body. It has a lot of porosities of 30,000 to 150,000 per 1 cm ³ , The present invention has the effect of reducing the number of bacteria and somatic cells in dairy wax, promoting growth, increasing the flow rate retention rate, removing odor and preventing mastitis, shortening the fattening period, It acts as a buffer against ruminants. In addition, it has an ammonia deodorizing function to reduce the smell of manure, thereby improving the housing environment.

The phyllotaxis contains Tetrathiobacter Kashmirensis and Streptomyces aureorectus. Tetrathiobacter cicamylensis is a sulfuric acid bacterium and contains 3.3 × 10 ⁷ cfu / g or more in phyllotaxis. It has an effect of decomposing sulfur and decomposing organic matter in the soil and neutralizing the acidity of the soil to improve the soil. Streptomyces aureolectus is actinomycetes and contains more than 8.3 × 10 ⁷ cfu / g in phyllite, and it has an action to prevent bulbous rot, anthracnose, antiseptic and ginseng spot and to decompose organic matter in the soil.

The phyllite is crushed and used in powder form.

② Limestone

Limestone is added as a source of calcium and used in powder form.

③ Sulfur

Sulfur is used in powder form in which the toxicity has been removed and added so that the ratio of nitrogen (N) to sulfur (S) in the feed composition is 13: 1. When the ratio of nitrogen and sulfur in the feed is adjusted as described above, the excess protein in the body is used to make the cow fat and improve meat quality.

④ Vitamins

Vitamins are used as nutritional ingredients and are used in feed for poultry (poultry, cow fattening) and breeding cattle breeding, but not for later frying (post-cow fattening, cow fattening).

⑤ Salt

Salt is added as a sodium source.

⑥ Probiotics

The probiotic agent was 8 to 13 parts by weight of pineapple; 8 to 13 parts by weight of rice straw; 8-13 parts by weight of phyllite; 20 to 42 parts by weight of a herbal medicinal herb including bellflower, bellflower, 20 to 42 parts by weight of corn meal; 8 to 13 parts by weight of vitamin B by-products are cultivated at 30 to 35 ° C for 2 to 5 days, and the cultured raw materials are mixed and fermented by fermentation at 28 to 32 ° C for 5 to 10 days .

First, pineapple juice used as a raw material for probiotics; Rice straw; Phyllomas (CMH); Herbal medicines including bellflower, dodeca, and daphnia; Corn meal and vitamin B by-products, respectively.

The pineapple juice contains a large amount of lactic acid bacteria such as Lactobacillus and Leuconostoc, which can be used as a nutrient source for plants, especially fibrin.

Rice straw contains a lot of Bacillus subtilis.

The phyllotaxis contains a lot of tetrathiobacteriocyme lysine and streptomyces aureolectus.

The herb medicine is preferably used in a mixture of 0.5 to 2 parts by weight of Bellflower, Doduck, and Daphniaceae.

Corn sickle rice is a remnant by-product of corn syrup containing Bacillus amyloliquefaciens, Saccharomyces cerevisiae, Aspergillus oryzae, Benaglucan, Beta amylase, and Alpha amylase.

Vitamin B by-products are by-products of vitamin B production.

Each of the raw materials is cultured at 30 to 35 DEG C for 2 to 5 days, preferably for 3 days. Each of the cultured raw materials was mixed with 8 to 13 parts by weight of pineapple; 8 to 13 parts by weight of rice straw; 8-13 parts by weight of phyllite; 20 to 42 parts by weight of a herbal medicinal herb including bellflower, bellflower, And 20 to 42 parts by weight of corn meal, followed by fermentation at 30 to 35 ° C. for 6 to 10 days to obtain a probiotic for use in the present invention. More preferably, the fermentation is aged for 7 days.

The probiotic is preferably a Bacillus subtilis (Bacillus Subtilis), Bacillus piece nipo miss (Bacillus Licheniformis), Bacillus Sono alkylene sheath (Bacillus Sonorensis), Bacillus core oyster lance (Bacillus Coagulans), Bacillus amyl Raleigh quinolyl Pacific Enschede ( Bacillus Amyloliquefaciens), Phedi O Lactococcus pen soil three mouse (Pediococcus Pentosaceus), three Levy in my process jiae Saccharomyces (Saccharomyces Cerevisiae ), Tetrathiobacter Kashimirensis, sulfate hwagyun), Streptomyces brother Leo rack tooth (Streptomyces Aureorectus , actinomycetes), and Fanny bacillus cookies ( Paenibacillus cookii ), more preferably at least eight microorganisms among the microorganisms listed above.

(4) Cattle feed  Preparation of composition

When the forage, concentrated feed and additives are mixed for about 20 minutes, a fever occurs at 30 to 40 ° C, and the probiotics are activated by fermentation and fermentation occurs.

The fermentation is stabilized when the fermentation proceeds for about two days in summer and one week in winter, so that the state of the feed composition can be maintained for one year or for a long time.

It is preferable that the water content of the feed composition is adjusted to be about 35% or less.

2. Cattle breeding methods

The cattle breeding method of the present invention is characterized in that,

15 to 25 parts by weight of a feedstock having at least one selected from the group consisting of Sudan grass, Holly rice, rice straw and oat hay; 50 to 70 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of palm, crushed corn, barley brewed poultry, embryo, energy complex, bread, corn gluten feed, pineapple, And 8 to 12 parts by weight of a first additive to a poultry meat; And

10 to 20 parts by weight of a forage which is at least one selected from the group consisting of Sudan grass, Holli rice, rice straw and oat hay; 60 to 80 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, barley brewed poultry, embryo puck, energy complex, bread, corn gluten feed, pineapple, And 8 to 12 parts by weight of a second additive at a later stage of finishing.

At this time, the first additive is a mixture of 10-20 parts by weight of phyllite, 6-10 parts by weight of limestone, 10-30 parts by weight of sulfur, 3-7 parts by weight of vitamin, 2-3 parts by weight of salt and 70-90 parts by weight of probiotics , ≪ / RTI >

The second additive is a mixture comprising 11 to 21 parts by weight of phyllotaxis, 7 to 11 parts by weight of limestone, 1 to 3 parts by weight of sulfur, 2 to 4 parts by weight of salt and 75 to 95 parts by weight of a probiotic agent,

The prophylactic agent is 8 to 13 parts by weight of pineapple; 8 to 13 parts by weight of rice straw; 8-13 parts by weight of phyllite; 20 to 42 parts by weight of a herbal medicinal herb including bellflower, bellflower, And 20 to 42 parts by weight of corn meal, respectively. The raw materials are cultured at 30 to 35 ° C for 2 to 5 days, and then the cultured raw materials are mixed and fermented for aging at 28 to 32 ° C for 5 to 10 days.

Explanation of each of the forage, concentrated feed, and additive is the same as described in the above feed composition.

Fertilization (6 months) is a shelf-life period of 6 months, at which time the weight of the cattle is generally between 375 and 580 kg. In the fattening process, it is preferable to feed the cattle feed composition of the present invention at 12 to 16 kg per day. By feeding the feed composition for poultry, the protein supply and the energy source supply are balanced to increase the fillet cross-sectional area.

The latter period (6 months) is the period of 7 ~ 12 months of standing, at which time the weight of cattle is generally 580 ~ 690kg. In the latter stage of fattening, it is preferable to feed the cattle feed composition of the present invention for 16 to 17 kg per day. By feeding the feed composition for later breeding period, the increase of the bypass protein content increases the production of the meat, and the meat and the meat quality are improved to the highest energy level.

The feed composition of the present invention is fed and fed in accordance with a conventional breeding method.

3. Beef

The feed composition of the present invention is fed to cattle and fed so that the amount of oleic acid contained in the fatty acid is 50 to 65% and the content of the unsaturated fatty acid is 55 to 70%, the crude saponin is 4 to 7% by weight (based on the building) Based on the building) beef can be obtained.

Hereinafter, the present invention will be described in more detail with reference to examples. These examples illustrate the present invention and are not intended to limit the scope of the present invention.

< Example  1>

Production of probiotics

30kg of herbal medicines mixed with pineapple juice 10kg, rice straw 10kg, phyllocarcinoma 10kg, bellflower roots, dodeca, and dangjuk herb were mixed at 30 ~ 35 ℃ for 30kg, 30kg of corn meal, Lt; / RTI &gt; Then, the cultured raw materials were mixed and fermented at 30 to 35 ° C for 7 days to obtain probiotics.

< Example  2>

Manufacture of energy complex

Energy complexes were prepared by mixing 20 kg of rice bran, 25 kg of olive oil, 15 kg of walnut oil, 10 kg of prunes, 7 kg of sunflower seeds, 3 kg of outline soybean and 20 kg of almond hull.

< Example  3>

1. For nonfarming and breeding Cattle feed  Preparation of composition

900kg of round rice straw as a roughage; 2,670 kg of concentrated feed consisting of 200 kg of palm, 330 kg of embryo, 440 kg of the energy complex prepared in Example 2, 360 kg of bread, 900 kg of pineapple and 440 kg of oil cake; And 450 kg of an additive composed of 60 kg of phyllite, 32 kg of limestone, 8 kg of sulfur, 9 kg of vitamin, 10 kg of salt, and 320 kg of the probiotics obtained in Example 1 were mixed and tightly packed after controlling moisture.

2. For the post-finishing period Cattle feed  Preparation of composition

600kg rounded rice straw for the forage; 2,670 kg of concentrated feed consisting of 200 kg of palm, 330 kg of embryo, 440 kg of the energy complex prepared in Example 2, 360 kg of bread, 900 kg of pineapple and 440 kg of oil cake; And 430 kg of an additive composed of 60 kg of phylloplast, 32 kg of limestone, 8 kg of sulfur, 10 kg of salt and 320 kg of the probiotics obtained in Example 1 were mixed, compressed, and moisture-tightly packed.

< Example  4>

The feed composition of Example 3 was fed to cattle and fed.

Fattening electric (stocked to 6 months), the third embodiment fattening electrical and yukjongyong was the feed composition salary per person 12 ~ 16kg per day of fattening reviews (stocking 7 to 12 months) are per person a feed composition for finishing late on day 16 ~ 17kg.

< Experimental Example  1>

The fatty acid composition of the feed composition prepared in Example 3 was analyzed by Chungnam National University Agricultural Research Institute, and the results are shown in Table 1 below.

fatty acid Composition (g / 100 g fatty acid) Capric acid 0.07 Lauric acid 2.64 Myristic oleic acid (14: 1) 1.97 Palmitic acid (16: 0) 22.35 Palmitoylic acid (16: 1) 0.33 Stearic acid (18: 0) 4.33 Oleic acid (18: 1 n 9c) 22.51 Linoleic acid (18: 2n6c) 48.88 Linolenic acid (18: 3n3) 3.44 ? -linolenic acid (18: 3n6) 0.54 Arachidic acid ((20: 0) 0.00 Behenan (24: 0) 0.5 Lignoceric acid (24: 0) 0.52 Percentage of saturated fatty acids (%) 32.67 Unsaturated fatty acid ratio (%) 75.35

< Experimental Example  2>

The beef samples obtained from the slaughtered cattle after being raised according to the method of Example 4 were submitted to the agricultural research institute of Chungnam National University for analysis of the components by 2016.08.12, and the results are shown in Table 2 below.

division Fatty acid name The Fatty acid composition
(g / 100 g fatty acid) saturation
fatty acid Capric acid C _{10: 0} 0.04 Lauric acid C _{12: 0} 0.10 Myristic acid C _{14: 0} 4.18 Pentadecanoic acid &lt; RTI ID = 0.0 &gt; C _{15: 0} 0.27 Palmitic acid C _{16: 0} 23.21 Magaric acid C _{17: 0} 0.44 Stearic acid C _{18: 0} 8.04 Arachidic acid C _{20: 0} 0.04 Unsaturated fatty acid


Myristoleic acid C _{14: 1} 2.05 Pentadecenoic acid &lt; RTI ID = 0.0 &gt; C _{15: 1} 0.10 Palmitoleic acid C _{16: 1} 6.49 Magaoleic acid C _{17: 1} 0.70 Oleic acid C _{18: 1 n9} 50.04 Linoleic acid _{C18: 2n6} 2.29 Linolenic acid _{C18: 3n3} 0.76 Stearidonic acid _{C18: 4n3} 0.01 Eicosenoic acid C _{20: 1 n 9} 0.43 Eicosadienoic acid _{C20: 2n6} 0.08 Dihomo gamma-linoleic acid &lt; RTI ID = 0.0 &gt; _{C20: 3n6} 0.01 Eicosatrienoic acid &lt; RTI ID = 0.0 &gt; _{C20: 3n3} 0.01

< Experimental Example  3>

The two beef samples obtained from the slaughtered cattle after being raised according to the method of Example 4 were submitted to the Agricultural Research Institute of Chungnam National University for the analysis of the components of the beef cattle in 2016. The results are shown in Table 3 below.

division Fatty acid name The Fatty acid composition
(g / 100 g fatty acid) Sample 1 Sample 2 saturation
fatty acid Capric acid C _{10 : 0} 0.04 0.03 Lauric acid C _{12 : 0} 0.15 0.13 Myristic acid C _{14 : 0} 3.18 2.77 Pentadecanoic acid C _{15 : 0} 0.14 0.14 Palmitic acid C _{16 : 0} 24.43 23.65 Magaric acid C _{17 : 0} 0.56 0.58 Stearic acid C _{18 : 0} 9.20 11.90 Arachidic acid C _{20 : 0} 0.21 0.14 Unsaturation
fatty acid Myristoleic acid C _{14 : 1} 1.09 0.91 Pentadecenoic acid C _{15 : 1} 0.13 0.14 Palmitoleic acid C _{16 : 1} 4.50 3.52 Magaoleic acid C _{17 : 1} 0.49 0.57 Oleic acid C _{18: 1 n9} 50.00 50.28 Linoleic acid C _{18 : 2 n 6} 2.17 1.72 Gamma-linolenic acid _{C18 : 3 n6} 0.11 0.08 Linolenic acid C _{18: 3 n3} 0.77 0.62 Stearidonic acid C _{18 : 4} 0.05 0.08 Eicosenoic acid C _{20: 1 n9} 0.43 0.46 Eicosadienoic acid C _{20 : 2 n 6} 0.09 0.11 Dihomo gamma-linoleic acid &lt; RTI ID = 0.0 &gt; C _{20 : 3 n 6} 0.11 0.14

< Experimental Example  4>

Two kinds of beef samples obtained from the slaughtered cattle after being raised according to the method of Example 4 were submitted to the Institute of Agricultural Science, Chungnam National University for the analysis of components by 2016.11.01. The results are shown in Table 4 below.

division Fatty acid name The Fatty acid composition
(g / 100 g fatty acid) Sample 1 Sample 2 saturation
fatty acid Capric acid C _{10 : 0} 0.04 0.04 Lauric acid C _{12 : 0} 0.08 0.09 Myristic acid C _{14 : 0} 2.17 2.26 Pentadecanoic acid C _{15 : 0} 0.07 0.08 Palmitic acid C _{16 : 0} 20.91 20.65 Magaric acid C _{17 : 0} 0.42 0.47 Stearic acid C _{18 : 0} 13.51 13.10 Arachidic acid C _{20 : 0} 0.05 0.06 Unsaturation
fatty acid Myristoleic acid C _{14 : 1} 0.32 0.36 Pentadecenoic acid C _{15 : 1} 0.07 0.09 Palmitoleic acid C _{16 : 1} 2.76 2.87 Magaoleic acid C _{17 : 1} 0.58 0.59 Oleic acid C _{18: 1 n9} 53.65 53.38 Linoleic acid C _{18 : 2 n 6} 2.55 2.74 Gamma-linolenic acid _{C18 : 3 n6} 0.10 0.09 Linolenic acid C _{18: 3 n3} 0.44 0.49 Stearidonic acid C _{18 : 4} 0.05 0.08 Eicosenoic acid C _{20: 1 n9} 0.57 0.62 Eicosadienoic acid C _{20 : 2 n 6} 0.11 0.11 Dihomo gamma-linoleic acid &lt; RTI ID = 0.0 &gt; C _{20 : 3 n 6} 0.10 0.01 Saturated fatty acid: unsaturated fatty acid ratio 37.80: 62.20 37.43: 62.57

< Experimental Example  5>

The components of four kinds of beef cattle (Test Examples 1 to 4) obtained from the four slaughtered cattle after being raised according to the method of Example 4 were analyzed in March 2016 by the Institute of Agricultural Science, Chungnam National University, Comparative Example 3 (3 other companies), Comparative Example 4 (Comparative Example 2), Comparative Example 5 (Comparative Example 5: 1 non-Hanwoo beef ), And Comparative Example 6 (third-party 2 brand beef). The results are shown in Table 5 below.

ingredient exam
Example 1 exam
Example 2 exam
Example 3 exam
Example 4 compare
Example 1 compare
Example 2 compare
Example 3 compare
Example 4 compare
Example 5 compare
Example 6 Crude saponin (%) 4.54 5.09 6.19 5.66 brimstone(%) 0.29 0.30 0.19 0.21 Capric acid (10: 0) 1.81 1.68 1.29 1.31 Lauric acid (12: 0) 0.30 0.14 0.08 0.10 26.35 22.48 20.31 19.14 0.00 0.15 Pentadecanoic acid
(15: 0) 0.29 0.20 0.20 0.18 Myristic acid
(14: 0) 2.71 1.75 1.80 1.76 Myristoleic acid
(14: 1) 0.69 0.85 0.81 0.55 10.51 9.18 8.61 7.83 1.57 1.27 Palmitic acid (16: 0) 22.97 18.75 20.93 19.91 14.11 15.81 15.86 15.44 28.02 33.03 Palmitooleic acid
(16: 1) 3.45 3.95 4.54 3.26 Mount Magar (17: 0) 0.68 0.75 0.85 0.73 Stearic acid
(18: 0) 12.65 10.47 9 10.96 3.18 3.28 3.43 3.70 11.03 12.87 Oleic acid
(18: 1 n 9c) 54.80 55.63 58.11 61.31 20.45 21.87 22.48 23.41 49.39 51.25 Linoleic acid
(18: 2n6c) 5.55 8.76 5.86 4.95 29.79 31.77 34.79 32.84 2 1.67 Linolenic acid
(18: 3n3) 0.48 0.49 0.33 0.58 1.47 1.53 1.62 4.35 - - Erus acid (22: 1) 0.50 0.47 0.51 0.42 Arachidonic acid
(20: 4n6) 0.93 0.84 1.07 0.59 Arachidic acid
(20: 0) 0.74 0.62 0.58 0.90 Other 3.34 6.4 5.41 3.85 Saturated fatty acid
ratio(%) 42.92 36.94 37.05 37.12 55.94 52.44 49.47 47.40 40.61 47.39 Unsaturated fatty acid
ratio(%) 67.09 73.07 72.96 72.89 51.70 55.17 58.88 60.59 52 47.78

As can be seen from the results of Table 5, in the beef of Test Examples 1 to 4 obtained in the Hanwoo which was fed with the feed composition of the present invention, the content of oleic acid influencing the taste of the beef was 54.80 to 61.31% . The ratio of fatty acids was lower than that of the comparative examples, and the proportion of unsaturated fatty acids was higher than that of the comparative examples. The oleic acid content and the proportion of the fatty acid contained in Test Examples 1 to 4 exceeded the level of Japan FUJI.

In addition, crude saponin (4.54 to 6.19%) and sulfur (0.19 to 0.30%) were detected in beef of Test Examples 1 to 4, but saponin and sulfur components were not detected in Comparative Examples.

< Experimental Example  6>

Comparative Example 8 (cow, Korea), Comparative Example 9 (Huawei, Japan) and Comparative Example 10 (United States) were used in addition to Test Examples 1 to 4 and Comparative Examples 5 and 6 of Experimental Example 5, And Comparative Example 11 (Australia) were added, and the content of oleic acid was analyzed. The results are shown in Table 6 below.

sample Oleic acid content Test Example 1 54.80 Test Example 2 55.63 Test Example 3 58.11 Test Example 4 61.31 Comparative Example 5 44.90 Comparative Example 6 46.59 Comparative Example 7 48 Comparative Example 8 27 Comparative Example 9 50.2 Comparative Example 10 42.5 Comparative Example 11 31.6

From the results shown in Table 6, it was confirmed that the content of oleic acid in the hanwoo meat obtained by feeding the feed composition of the present invention was 54.80 ~ 61.31%, which is significantly higher than those of the other comparative examples including Japanese white beef.

< Experimental Example  7>

The content of omega-3 (N-3) and omega-6 (N-6) fatty acids in the Hanwoo and Oatmeal of Experimental Examples 1 to 4 was analyzed by Chungnam National University Agricultural Research Institute. For comparison, Comparative Example 1 (3 other companies), Comparative Example 3 (3 other companies), and Comparative Example 4 (2 other companies) ) Were analyzed in the same manner.

The results are shown in Table 7 below.

division Test Example Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 N-3 fatty acids (%) 3.12 1.33 1.39 1.47 3.95 N-6 fatty acids (%) 44.92 27.08 28.88 31.61 29.85 ratio 1: 14.397 1: 20.360 1: 20.776 1: 21.503 1: 7.556

The results of Table 7 show that the ratio of omega-3 fatty acid and omega-6 fatty acid in beef of the present invention is superior to the comparative examples.

< Experimental Example  8>

Phyllite  Microbial component analysis

The microorganism components of phyllite used in Example 1 were analyzed for representative effective strains. The analysis was commissioned by the Institute of Microbiology and Ecology, Mokwon University, and the results are shown in Table 8 below.

Effective microorganism Assured live bacteria count Number of live bacteria Tetrathiobacteriocamilensis 3.3 x 10 &lt; ⁷ &gt; cfu / g 7.3 x 10 &lt; ⁷ &gt; cfu / g Streptomyces aureotactus 8.3 x 10 &lt; ⁷ &gt; cfu / g 8.4 x 10 &lt; ⁷ &gt; cfu / g

< Experimental Example  9>

1 Probiotic  Microbial component analysis

The microbial component of the probiotics prepared in Example 1 was analyzed. The analysis was carried out on the three samples obtained in the same manner as in Example 1. The results are shown in Tables 9 to 11 below. (Please inform the analytical agency if you are commissioned outside)

Inspection items unit test results Bacillus Subtilis cfu / g 3.0 × 10 ⁵ Bacillus ricinifomis cfu / g 2.0 × 10 ⁵ Pediococcus pentosaceus cfu / g 2.0 × 10 ⁷ Sakaromasse Serebijia cfu / g 4.0 × 10 ⁶

Inspection items unit test results Bacillus Subtilis cfu / g 1.0 × 10 ⁵ Bacillus amyloliquefaciens cfu / g 3.0 × 10 ⁵ Paenibacillus Kukai cfu / g 3.0 × 10 ⁵ Pediococcus pentosaceus cfu / g 3.0 × 10 ⁸ Sakaromasse Serebijia cfu / g 3.0 × 10 ⁶

Inspection items unit test results Bacillus Subtilis cfu / g 2.0 × 10 ⁵ Bacillus sonorensis cfu / g 2.0 × 10 ⁵ Bacillus coagulans cfu / g 1.0 x 10 ⁴ Pediococcus pentosaceus cfu / g 3.0 x 10 ⁷ Sakaromasse Serebijia cfu / g 3.8 × 10 ⁶

As can be seen from Tables 9 to 11, the probiotics used in the present invention contained a large amount of various effective microorganisms.

< Experimental Example  10>

Cow breeding

For 12 months from stocking up to 12 months, cows were raised using the feed composition of the present invention as follows (unit: kg).

Stocking age One 2 3 4 5 6 7 8 9 10 11 12 Foundation weight 345 375 410 445 480 515 550 580 605 630 650 670 Term weight 375 410 445 480 515 550 580 605 630 650 670 690 Weight gain 30 35 35 35 35 35 30 25 25 20 20 20 Daily gain One 1.1 1.1 1.1 1.1 1.1 One 0.8 0.8 0.7 0.7 0.7 Fetal Electric Intake / Day 12 ~ 16 / day - Fertility intake / day - 16-17 / day Electricity consumption / month 434 / month - Electric Intake / Subtotal 2,604 - Late intake / month - 512 / month Late intake / Subtotal - 3,072 Total intake 5,676

Claims (12)

15 to 25 parts by weight of a feedstock for at least one selected from the group consisting of Sudan grass, Holly rice, rice straw and oat hay; 50 to 70 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo puck, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of the first additive,
For the latter, 10 to 20 parts by weight of a forage, which is at least one selected from Sudan grass, Holli rice, rice straw and oat hay; 60 to 80 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of a second additive,
The first additive is contained in a ratio of 10 to 20 parts by weight of phyllite, 6 to 10 parts by weight of limestone, 1 to 4 parts by weight of sulfur, 1 to 5 parts by weight of vitamin, 1 to 5 parts by weight of salt, and 65 to 90 parts by weight of a probiotic Lt; / RTI &gt;
The second additive is a mixture comprising 11 to 21 parts by weight of phyllite, 7 to 11 parts by weight of limestone, 1 to 6 parts by weight of sulfur, 1 to 5 parts by weight of salt, and 70 to 95 parts by weight of a probiotic agent,
The prophylactic agent is 8 to 13 parts by weight of pineapple; 8 to 13 parts by weight of rice straw; 8-13 parts by weight of phyllite; 20 to 42 parts by weight of a herb medicine containing bellflower, dodeca, 20 to 42 parts by weight of corn meal; 8 to 13 parts by weight of vitamin B by-products are cultured at 30 to 35 DEG C for 2 to 5 days, and then the cultured raw materials are mixed and fermented and aged at 28 to 32 DEG C for 5 to 10 days. Cattle feed composition for improving meat quality. The method according to claim 1,
The concentrated feed contains 15 to 25 parts by weight of a palm or ground corn, 28 to 38 parts by weight of an embryo, 39 to 49 parts by weight of an energy complex, 31 to 41 parts by weight of bread, 85 to 95 parts by weight of pineapple, , Wherein the composition for improving meat quality comprises at least one component selected from the group consisting of: The method according to claim 1,
The first additive is a solid probiotics obtained by aging the fermented rocks, limestone, sulfur, vitamins, salt, and probiotics in a fermentation storage at 20 to 35 ° C for 5 days or more,
Wherein the second additive is a solid probiotics obtained by aging the fermented rock, limestone, sulfur, salt, and probiotics in a fermentation storage at 20 to 35 ° C for 5 days or more. 4. The method according to any one of claims 1 to 3,
The probiotics may be selected from the group consisting of Bacillus subtilis, Bacillus ricinifomis, Bacillus sonolenesis, Bacillus coagulans, Bacillus amyloliquequiassense, Pediococcus pentosaceus, Saccharomyces cerevisiae, Wherein the microorganism comprises at least five microorganisms selected from the group consisting of mylensis, streptomyces aureolectus, and fanny bacillus cookies. 4. The method according to any one of claims 1 to 3,
The energy complex comprises 18 to 22 parts by weight of rice bran, 23 to 27 parts by weight of olive, 13 to 17 parts by weight of walnut, 8 to 12 parts by weight of prunes, 6 to 7 parts by weight of sunflower seed, 2 to 4 And 18 to 22 parts by weight of an almond mortar. 4. The method according to any one of claims 1 to 3,
Wherein the forage is comprised of rice straw or rice straw and orchard hay. 15 to 25 parts by weight of a feedstock having at least one selected from the group consisting of Sudan grass, Holly rice, rice straw and oat hay; 50 to 70 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo puck, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of a first additive to a poultry meat; And
10 to 20 parts by weight of a forage which is at least one selected from the group consisting of Sudan grass, Holli rice, rice straw and oat hay; 60 to 80 parts by weight of a concentrated feed having three or more essential components selected from the group consisting of crushed corn, crushed corn, barley brewed poultry, embryo pile, energy complex, bread crumb, corn gluten feed, pineapple, And 8 to 12 parts by weight of a second additive at a later stage of the frying,
The first additive is contained in a ratio of 10 to 20 parts by weight of phyllite, 6 to 10 parts by weight of limestone, 1 to 4 parts by weight of sulfur, 1 to 5 parts by weight of vitamin, 1 to 5 parts by weight of salt, and 65 to 90 parts by weight of a probiotic Lt; / RTI &gt;
The second additive is a mixture comprising 11 to 21 parts by weight of phyllite, 7 to 11 parts by weight of limestone, 1 to 6 parts by weight of sulfur, 1 to 5 parts by weight of salt, and 70 to 95 parts by weight of a probiotic agent,
The prophylactic agent is 8 to 13 parts by weight of pineapple; 8 to 13 parts by weight of rice straw; 8-13 parts by weight of phyllite; 20 to 42 parts by weight of a herb medicine containing bellflower, dodeca, 20 to 42 parts by weight of corn meal; Vitamin B by-product 8-13 parts by weight Each of the raw materials is cultured at 30-35 ° C for 2-5 days, and the cultured raw materials are mixed and fermented for aging at 28-32 ° C for 5-10 days. Breeding methods. 8. The method of claim 7,
The concentrated feed contains 15 to 25 parts by weight of a palm or ground corn, 28 to 38 parts by weight of an embryo, 39 to 49 parts by weight of an energy complex, 31 to 41 parts by weight of bread, 85 to 95 parts by weight of pineapple, Wherein the method comprises the steps of: 8. The method of claim 7,
The first additive is a solid probiotics obtained by aging the fermented rocks, limestone, sulfur, vitamins, salt, and probiotics in a fermentation storage at 20 to 35 ° C for 5 days or more,
Wherein the second additive is a solid probiotics obtained by aging the fermented rock, limestone, sulfur, salt, and probiotics in a fermentation storage at 20 to 35 캜 for 5 days or more. 10. The method according to any one of claims 7 to 9,
Wherein the forage is comprised of rice straw or rice straw and orchard hay. 10. The method according to any one of claims 7 to 9,
Wherein the feed composition for poultry meat is fed at a feed rate of 12 to 16 kg / day, and the feed composition for late poultry is fed at a feed rate of 16 to 17 kg / day. delete