KR101697811B1 - cDNA 의 합성 방법 - Google Patents

cDNA 의 합성 방법 Download PDF

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Publication number
KR101697811B1
KR101697811B1 KR1020127032358A KR20127032358A KR101697811B1 KR 101697811 B1 KR101697811 B1 KR 101697811B1 KR 1020127032358 A KR1020127032358 A KR 1020127032358A KR 20127032358 A KR20127032358 A KR 20127032358A KR 101697811 B1 KR101697811 B1 KR 101697811B1
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KR
South Korea
Prior art keywords
endo
dnase
reverse transcriptase
dna
reaction
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KR1020127032358A
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English (en)
Korean (ko)
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KR20130062296A (ko
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유코 나카바야시
다카시 우에모리
히로유키 무카이
기요조 아사다
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다카라 바이오 가부시키가이샤
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Publication of KR20130062296A publication Critical patent/KR20130062296A/ko
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Publication of KR101697811B1 publication Critical patent/KR101697811B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020127032358A 2010-05-14 2011-05-09 cDNA 의 합성 방법 KR101697811B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2010111595 2010-05-14
JPJP-P-2010-111595 2010-05-14
PCT/JP2011/060643 WO2011142310A1 (ja) 2010-05-14 2011-05-09 ｃＤＮＡの合成方法

Publications (2)

Publication Number Publication Date
KR20130062296A KR20130062296A (ko) 2013-06-12
KR101697811B1 true KR101697811B1 (ko) 2017-01-18

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KR1020127032358A KR101697811B1 (ko) 2010-05-14 2011-05-09 cDNA 의 합성 방법

Country Status (6)

Country Link
US (1) US9169482B2 (de)
EP (1) EP2570480B1 (de)
JP (1) JP5940447B2 (de)
KR (1) KR101697811B1 (de)
CN (1) CN102884186B (de)
WO (1) WO2011142310A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111733213A (zh) * 2019-03-25 2020-10-02 天根生化科技（北京）有限公司 反转录反应液及制备cDNA的方法
CN110229809A (zh) * 2019-06-20 2019-09-13 中国农业科学院兰州兽医研究所 一种Rochel反转录试剂及其制备方法
WO2021231706A1 (en) * 2020-05-13 2021-11-18 The Trustees Of Columbia University In The City Of New York Methods and compositions for rapid testing for a target nucleic acid including viral nucleic acid

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005304396A (ja) * 2004-04-22 2005-11-04 Toyobo Co Ltd 偽陽性を低減可能なリボ核酸増幅試薬組成、およびそれを用いたリボ核酸の増幅方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1004676B1 (de) 1997-08-14 2006-03-15 Takara Bio Inc. Methoden zur dns vervielfältigung und material dafür
US7264932B2 (en) * 1999-09-24 2007-09-04 Applera Corporation Nuclease inhibitor cocktail
CA2468145A1 (en) * 2001-11-21 2003-05-30 Japan Science And Technology Agency Transcriptional factors for the huntington's disease gene
US20060223073A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
EP1707623A1 (de) 2005-04-01 2006-10-04 Qiagen GmbH Reverse Transkription und Amplifikation von RNA bei simultaner Degradierung von DNA
US8309303B2 (en) 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
JP5073967B2 (ja) 2006-05-30 2012-11-14 株式会社日立製作所 単一細胞の遺伝子発現定量方法
GB2474225A (en) * 2009-07-21 2011-04-13 Biotec Pharmacon Asa DNase for decontamination of reverse transcription and amplification reactions

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005304396A (ja) * 2004-04-22 2005-11-04 Toyobo Co Ltd 偽陽性を低減可能なリボ核酸増幅試薬組成、およびそれを用いたリボ核酸の増幅方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Jen-Yang Chen 등. Journal of Virological Methods. Vol 45, No. 1, 페이지 49-66 (1993.)*

Also Published As

Publication number Publication date
CN102884186B (zh) 2015-01-28
US20130065281A1 (en) 2013-03-14
JP5940447B2 (ja) 2016-06-29
CN102884186A (zh) 2013-01-16
EP2570480B1 (de) 2018-07-04
EP2570480A4 (de) 2014-03-05
KR20130062296A (ko) 2013-06-12
JPWO2011142310A1 (ja) 2013-07-22
US9169482B2 (en) 2015-10-27
EP2570480A1 (de) 2013-03-20
WO2011142310A1 (ja) 2011-11-17

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