KR101631453B1 - Composition for Protecting Ultraviolet Rays from Damaging Skin Originated from Extracts of Plants or Fermentation Thereof and Method for Producing the Same - Google Patents

Abstract

The present invention relates to a composition for extracting a plant material or an ultraviolet screening agent composition using the same and a method for preparing the same. More specifically, the present invention relates to a plant extract or a fermented substance thereof as a main component of an effective ingredient extracted from a plant material or a fermented substance of an effective ingredient And a method for producing the same. Wherein the extract is at least one member selected from the group consisting of Phellinus lanceolata, Corynebacterium spp., Mycobacterium bark, bamboo, wool, whitening, cactus and aloe, and at least one of the extracts is extracted by hot water extraction, ethanol extraction, microwave extraction and supercritical extraction, Wherein the fermented ingredient is fermented by Lactobacillus.

Description

FIELD OF THE INVENTION [0001] The present invention relates to an ultraviolet screening composition comprising a vegetable material extract or a fermented substance thereof and a method for producing the same.

The present invention relates to a composition for extracting a plant material or an ultraviolet screening agent composition using the same and a method for preparing the same. More specifically, the present invention relates to a plant extract or a fermented substance thereof as a main component of an effective ingredient extracted from a plant material or a fermented substance of an effective ingredient And a method for producing the same.

Ultraviolet Ray is UV-C that is completely absorbed by the stratospheric ozone, UV-B which is mostly absorbed by the ozone layer but partially reaches the surface, and has a longer wavelength than other kinds of ultraviolet light which is not absorbed by the ozone layer. UV-A. UV-A is known to cause skin cancer by causing redness of the skin and acting on the skin's immune system, causing long-term skin damage due to aging of the skin. UV-B is known to cause skin cancer by burning the skin of an animal and penetrating into skin tissue.

A synthetic filter for protecting the skin by changing ultraviolet rays to heat energy and a ultraviolet scattering agent for reflecting or scattering ultraviolet rays reaching the skin are known as methods for blocking ultraviolet rays that affect skin or body tissues have. Ultraviolet absorbers include para-amino benzoic acid and ultraviolet absorbers such as titanium dioxide and zinc oxide. Such an ultraviolet absorber or ultraviolet scattering agent has the disadvantage that it causes urticaria or inflammation in the sensitive skin or thickens the skin.

As a prior art related to an ultraviolet screening agent for solving such a problem, Patent Publication No. 2013-0087650 " Ultraviolet screening composition and its manufacturing method " The prior art discloses an ultraviolet screening composition comprising at least one ultraviolet screening activator selected from the group consisting of phenolic acid based compounds, flavonoid based compounds and derivatives thereof and a hydrophobic liquid polymeric medium for impregnating such ultraviolet screening active agents .

Other prior art related to sunscreen agents disclose sunscreen agents containing a coacebate in which an organic or inorganic sunscreen is captured.

Another prior art related to ultraviolet screening agents is Patent Disclosure No. 2008-0072997 " Water-soluble ultraviolet screening agent and its environmentally friendly manufacturing method ". The prior art discloses the use of cyclodextrins and 2,4- bis- (4- (2-ethyl-hexyloxy) -2-hydroiodide) -phenyl-6- (4-methoxyphenyl) - ) -Triazine, and the cyclotrityl and the ultraviolet absorber have a molar ratio of 3: 1.

The ultraviolet screening agents disclosed in the prior art are all inorganic or organic compounds, which makes it difficult to solve the problems of the conventional ultraviolet ray compound.

The present invention has been made to solve the problems of the prior art and has the following purpose.

It is an object of the present invention to provide an ultraviolet screening composition comprising a plant material extract or a fermented substance thereof.

It is another object of the present invention to provide a method for preparing an ultraviolet screening composition by extracting a plant material or a fermenting material thereof.

According to a preferred embodiment of the present invention, at least one selected from the group consisting of Porcupine, Centella orientalis, Mycobacterium, Bamboo, Wool, Cotton, Cactus and Aloe, and the at least one is selected from the group consisting of hot water extraction, ethanol extraction, microwave extraction, The extract or the fermentation component of the extract, wherein the fermentation component is fermented by Lactobacillus.

According to another preferred embodiment of the present invention, the extract or fermentation component comprises at least one selected from the group consisting of coumarinic acid, cinnamic acid, ferric acid, aciatic acid and asiaticoside And has a cytokine secretion inhibiting effect.

According to another preferred embodiment of the present invention, the extract or the fermented ingredient has a skin irritation relieving function or a skin irritation relieving function.

According to another preferred embodiment of the present invention, there is provided a method for preparing a plant material, comprising the steps of: preparing at least one vegetable material selected from the group consisting of Porcupineous, Centella asiatica, Corymbus, Bamboo, Wool, Cotton, Cacti and Aloe; Forming an extract from the at least one vegetable material by hot water extraction, ethanol extraction, microwave extraction or supercritical extraction; And forming a concentrated solution of the extract.

According to another preferred embodiment of the present invention, the extract is further fermented by Lactobacillus.

The composition according to the present invention has an advantage that it is produced from a plant material and does not cause side effects such as the occurrence of inflammation. Since the composition according to the present invention is a natural material, it has an advantage that it can be added to various functional natural cosmetic materials. In addition, the composition according to the present invention has an advantage that it can be easily removed from the skin after use with excellent feeling of use.

1 schematically illustrates a process for preparing a composition according to one embodiment of the present invention.
FIG. 2A shows the UVB blocking effect of the herbal extracts of hotpot and hot-water.
FIG. 2B is a graph showing the effect of UVB blocking on the extracts of Dongpu hot-water extract, fermented components, hydrothermal extracts and fermented components, and FIG. Figure 2d shows the effect of UVB blocking on the wort root solid fermentation component and the whitish solid fermentation component. And Fig. 2E shows the UVB blocking effect on the ethanol extract of cactus and the hot water extract of aloe.
3A to 3D show the UVA blocking effect, and Octocyrlene, which is an organic blocker, was used as a control.
In Figs. 3B to 3D, the left side shows the extract, the right side shows the fermentation component, and the lower side shows that the extract does not contain the fermentation component.
Figure 4 shows the test results of the pharmaceutical efficacy of the compositions according to the invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail with reference to the embodiments shown in the accompanying drawings, but the present invention is not limited thereto. In the following description, components having the same reference numerals in different drawings have similar functions, so that they will not be described repeatedly unless necessary for an understanding of the invention, and the known components will be briefly described or omitted. However, It should not be understood as being excluded from the embodiment of Fig.

The ultraviolet screening composition according to the present invention comprises at least one selected from the group consisting of Porphyrae, Centella asiatica, Mykiss, Bamboo, Wool, Cotton, Cactus and Aloe as hot water extraction, ethanol extraction, microwave extraction and supercritical extraction Or the fermentation component of the extract, wherein the fermentation component is fermented by Lactobacillus. Also, a method for preparing an ultraviolet screening composition using a plant material extract may include preparing at least one vegetable material selected from the group consisting of Corynebacterium glutinosa, Centella asiatica, Corymbus, Bamboo, Wool, Cotton, Cactus and Aloe; Forming an extract from the at least one vegetable material by hot water extraction, ethanol extraction, microwave extraction or supercritical extraction; Forming a concentrated solution of the extract; And fermenting the concentrated solution with Lactobacillus.

This is explained in detail below.

1 schematically illustrates a process for preparing a composition according to one embodiment of the present invention.

Referring to FIG. 1, a method of preparing an ultraviolet screening composition comprises preparing a vegetable material (S11); Extracting an effective ingredient from the vegetable material (S12); Forming a concentrated solution of the extracted active ingredient (S13); And fermenting the concentrated solution (S14).

The vegetable material for the production of the composition according to the present invention may be at least one selected from the group consisting of, for example, Porcupineous, Centella asiatica, Mykiss, Bamboo, Wool and Cotton. Poonggyo is a medicinal herb that has dried the outpost of a dandelion or an inbred plant of Asteraceae. Centella asiatica is a plant in which green leaves and stalks are used medicinally and is used as a medicine for skin wounds, chronic ulcers, arthritis, angulation or fever. The bedspread according to the present invention includes a bedspread that grows in any area. Ecklonia stolonifera is a perennial seaweed rich in minerals belonging to the seagrass and the seagrass. Bamboo is a plant belonging to the genus Picea. The composition according to the present invention may mainly comprise an extract of bamboo leaf. Petasites japonicus is a perennial herbaceous plant belonging to the family Asteraceae which grows naturally in the forests or around the valley, and the bud refers to a plant used as a medicinal herb, and the composition according to the present invention can mainly comprise an extract of a medicinal herb leaf. It is a plant belonging to the cabbage family, which belongs to evergreen coniferous trees and is resistant to cold tolerance and salt tolerance, but resistant to intractability. Anti-bacterial, anti-stress, deodorizing and cleansing function and immunity function are known to be contained in antibacterial substances called phytoncide, and the composition according to the present invention can mainly include an extract of Paeonia leaf .

On the other hand, cactus (Opuntia ficus-indica var, saboten MAKINO) corresponds to tropical plant herbaceous plants and mainly branches are used for the preparation of the composition according to the invention. Aloe is a plant belonging to the lily family, and about 300 species are present. The composition according to the present invention is not limited to the kind of aloe, but leaves and stems can be applied for the production of the composition.

For the preparation of the composition according to the present invention, each plant material can be harvested and the leaves, stems or roots can be washed and dried and crushed as required (S11).

The prepared vegetable material can be extracted according to an extraction method suitable for each material, but a method such as hot water extraction, ethanol extraction microwave extraction and supercritical extraction can be applied, for example, in consideration of the fact that it should be applied to the human body (S12 ). And the extract can be made into a concentrated solution (S13).

Specifically, the hot water extraction can be performed by, for example, making the distilled water at 80 to 100 ° C and immersing the soft white leaves for 2 to 6 hours. The extract can then be filtered and concentrated under reduced pressure. The ethanol extraction can be carried out by immersing the filamentous leaves in ethanol and incubating at 30 to 45 DEG C for 2 to 5 days, after which the extracted active ingredients are filtered and reduced in pressure and concentrated. Microwave extraction can be achieved by immersing the flat leaves in distilled water, microwaving for 10 to 50 minutes, filtering the extract, and concentrating under reduced pressure. And the supercritical extraction may be carried out in a manner such that carbon dioxide or pentane proceeds at a temperature of 300 to 400 bar at a temperature of 70 to 90 DEG C for 2 to 6 hours and the extraction efficiency is 0.2 to 0.5 wt% of the sample weight.

According to the present invention, the concentrated solution can be directly applied as a composition for ultraviolet ray shielding or fermented (S14) as a composition for ultraviolet ray shielding.

Bacteria such as, for example, Lactobacillus fermentum, Lactobacillus brevis, Lactobacillus plantarum or Aspergillus niger may be used for the fermentation . The fermentation can be carried out at a temperature of 20 to 35 ° C for 2 to 6 days, and the fermentation broth can be further centrifuged at 10,000 to 20,000 rpm for 10 to 30 minutes in a centrifuge. Thereafter, the supernatant separated by centrifugation may be concentrated.

On the other hand, solid fermentation can be applied to obtain the composition according to the present invention. Solid fermentation can result in the plant material being washed and dried, and the dried material finely comminuted to form a powder. The material in powder form can then be fermented with Lactobacillus bacteria or preferably Lactobacillus plantarum for 2 to 6 weeks in MRS medium. The fermentation can be carried out, for example, at 20 to 35 ° C. The fermented material may be dissolved in a suitable solvent and applied as a composition of an ultraviolet screening agent.

The fermentation component of the vegetable extract or vegetable extract described above may include, for example, a substance such as coumarinic acid, cinnamic acid or ferric acid, and may include organic acids, sugars, saponins, alkaloids, glycosides, . It may also contain materials such as acic acid or asiaticoside. The composition according to the invention may also be mixed with other suitable materials and used as an ultraviolet screening agent.

An example of a sunscreen agent containing the composition according to the present invention will be described below.

Step 1: Forage leaves were collected, washed, dried and finely ground.

Step 2: The ground material was immersed in distilled water at about 85 캜 for about 3 hours, filtered and concentrated under reduced pressure (about 4.57 wt% based on the total weight).

Step 3: 5.0 g of the material under reduced pressure, 10 g of propylene glycol, 1.0 g of castor oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Step 1: The collecting beds were collected, washed, dried and ground finely.

Step 2: The pulverized material was immersed in ethanol at about 40 占 폚 for 2 days, and the filtrate was concentrated under reduced pressure (about 4.54 wt% based on the total weight).

Step 3: 5.0 g of the material under reduced pressure, 10 g of propylene glycol, 10 g of mineral oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Step 1: The ginseng was collected, washed, dried and ground finely.

Step 2: The pulverized material was placed in distilled water, treated with microwave for 30 minutes at a temperature of about 60 DEG C, filtered and concentrated under reduced pressure (about 4.21 wt% based on the total weight).

Step 3: 5.0 g of the material under reduced pressure, 10 g of propylene glycol, 1.0 g of castor oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Step 1: Bamboo leaves were collected, washed, dried and ground finely.

Step 2: The pulverized material was treated with carbon dioxide at a temperature of 350 bar and 80 ° C for 3 hours so that the extraction efficiency was 0.3 wt% of the total weight.

Step 3: 5.0 g of the obtained extract, 10 g of propylene glycol, 10 g of mineral oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Stage 1: Washes were collected, washed, dried and finely ground.

Step 2: The pulverized material was processed in the same manner as in Example 1 to obtain a vacuum concentrate, which was then fermented with Lactobacillus platelum at a temperature of 25 DEG C for 3 days and centrifuged to obtain a concentrate 2.41 wt%).

Step 3: 5.0 g of the obtained concentrate, 10 g of propylene glycol, 1.0 g of castor oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Step 1: Leaves were collected, washed, dried and ground finely.

Step 2: The pulverized material was fermented with Lactobacillus plantarum in MRS medium at 25 DEG C for 3 weeks to obtain a solid fermentation product, which was then dissolved in ethanol and centrifuged and filtered (about 3.25 wt% of the total weight).

Step 3: 5 g of the solid fermentation product, 10 g of propylene glycol, 10 g of mineral oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Step 1: Cactuses were collected, washed, dried and finely ground.

Step 2: The pulverized material was immersed in ethanol at about 40 占 폚 for 2 days, and the filtrate was concentrated under reduced pressure (about 4.60 wt% based on the total weight).

Step 3: 5.0 g of the material under reduced pressure, 10 g of propylene glycol, 10 g of mineral oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Step 1: Aloe was collected, washed, dried and finely ground.

Step 2: The pulverized material was put into distilled water, microwave-treated at a temperature of about 60 ° C for 30 minutes, filtered and the filtrate was concentrated under reduced pressure (about 4.81 wt% based on the total weight).

Step 3: 5.0 g of the material under reduced pressure, 10 g of propylene glycol, 1.0 g of castor oil, 0.2 g of citric acid and 60 g of purified water were mixed and stirred to prepare an ultraviolet screening agent.

Efficacy test

The ultraviolet screening agents obtained from Examples 1 to 8 were evaluated for their SPF index and safety. The concentrations of the ultraviolet screening agents obtained in Examples 1 and 8 were used as standard samples, and the concentrations of the extracts were further adjusted by adding the extracts.

A total of 100 stimuli were induced for 20 days by applying ultraviolet screening agent every 24 hours. As a control, zinc oxide (Zinc oxide) and titanium dioxide (TiO 2), which are inorganic UV blocking agents, were used.

A UV spectrometer and a Xenon Arc Lamp were used for the measurement, the measurement wavelength was 290 to 400 nm, and the SPF was measured 15 minutes after application of the sunscreen.

The test results of the breaking line breaking index (SPF) are as follows.

1 means the standard sample, and 2 and 4 mean that the concentration of the standard sample is doubled and quadrupled, respectively

In each test, human cumulative skin reactions were tested and classified by 0 to 4 grades (No visible reaction, Mild erythema, Intense erythema, Intense erythema with edema, Intense erythema with edema & vesicle) Trends in skin reactions were recorded according to water content and laser blood flow. There was no evidence of a person with a grade 1 or higher response.

On the other hand, UVA was irradiated and the light-toxicity index was calculated after 24 hours and 48 hours. Phototoxic index = the irradiance index of the UV irradiated area - the irradiance index of the UV unirradiated area.

The light-toxicity index can be expressed as a value from 0 to 8.0, 0 means no phototoxicity (Non Phototoxic) and 8.0 indicates extreme phototoxic. For the 100 test subjects, 20 out of the first survey showed a light-toxicity index of about 0.3.

On the other hand, the efficacy of other extracts according to the present invention was tested using a UV-sensitive yeast strain.

For the test, the ultraviolet screening agent prepared according to the examples was applied to the culture cover and irradiated with UVB and UVA for 2 minutes.

FIG. 2A shows the UVB blocking effect of the herbal extracts of hotpot and hot-water.

The hot water extraction was performed according to the method presented in the presented embodiment.

FIG. 2B is a graph showing the effect of UVB blocking on the extracts of Dongpu hot-water extract, fermented components, hydrothermal extracts and fermented components, respectively, and FIG. Figure 2d shows the effect of UVB blocking on the solid fermentation components and the waxy solid fermentation components of wool roots. And FIG. 2E shows the UVB blocking effect of the ethanol extract of cactus and the hot water extract of aloe.

In Fig. 2a, titanium dioxide and zinc oxide were used as inorganic phase steps and the concentrations of the standard samples presented in the examples were applied. 2b to 2e shows that the extract is represented by an extract, 2 is a fermentation ingredient, and 3 is an extract and fermentation ingredient.

3A to 3D show the UVA blocking effect, and Octocyrlene, which is an organic blocker, was used as a control. Octocrylene was applied at the standard concentration of the same example as the inorganic blocker. In Figs. 3B to 3D, the left side shows the extract, the right side shows the fermentation component, and the lower side shows that the extract does not contain the fermentation component. The extract and the fermented ingredient were prepared in the same manner as the examples shown in Figs. 2B to 2D.

On the other hand, in order to test the pharmaceutical properties of the composition according to the present invention, the compositions prepared according to Examples 1 to 8 were stimulated with LPS (lipopolysaccharide) on a HaCa T cell line to produce cytokines The change in the amount was measured. Each composition was used at a concentration of 0.1 [mu] l / ml and cytokine was measured by measuring the amount of interleukin-8 (IL-8) (interleukin) and the results are shown in FIG.

In Fig. 4, + represents + LPS, - represents -LPS, and 1 to 8 represent examples. As can be seen from FIG. 4, it can be seen that the amount of cytokine decreases with the composition according to Examples 1 to 8, and that the substance according to the present invention has anti-inflammatory or pharmacological effects.

As can be seen from the test examples presented, it can be seen that the composition according to the present invention has the same effect as the inorganic UVB ultraviolet screening agent or the organic UVA ultraviolet screening agent without inducing skin irritation. Therefore, it has an advantage that it can be an alternative component to this.

The composition according to the present invention has an advantage that it is produced from a plant material and does not cause side effects such as the occurrence of inflammation. Since the composition according to the present invention is a natural material, it has an advantage that it can be added to various functional natural cosmetic materials. In addition, the composition according to the present invention has an advantage that it can be easily removed from the skin after use with excellent feeling of use.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention . The invention is not limited by these variations and modifications, but is limited only by the claims appended hereto.

S11: Preparation of vegetable materials
S12: Extraction of active ingredient
S13: Concentrated solution formation
S14: Fermentation

Claims (5)

Collecting, washing, drying and finely crushing the mycelia;
Extracting the active ingredient from the ground beef fat and concentrating it under reduced pressure;
Fermenting the extracted extract with reduced pressure to a lactobacillus bacterium at a temperature of 20 to 35 캜 and making it into a fermentation concentrate by centrifugation; And
Mixing the fermentation concentrate with propylene glycol, castor oil, citric acid, and purified water. delete [Claim 2] The method according to claim 1, wherein the extraction of the active ingredient is achieved by adding the pulverized material to distilled water and treating the mixture with microwave for 30 minutes at a temperature of 60 [deg.] C and filtering. delete delete

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