KR101459331B1 - Preparation method of abalone mutant which grows faster - Google Patents

Abstract

The present invention relates to a method of producing a mutant abalone with a rapid growth rate. More specifically, the present invention relates to a method for producing mutant abalone, It relates to a method of producing abalone showing rapid growth in abalone. The growth-promoted overturned production method according to the present invention is a method for producing an abalone in which a mature abalone is placed in an abalone holding box and irradiated with a specific dose of gamma rays to thereby effectively immobilize the abalone having mobility, Thus, the mutation induction according to the irradiation of specific dose has the advantage of producing abalone which shows a rapid growth rate compared with the abalone raised by the general aquaculture method.

Description

[0001] The present invention relates to a method for producing mutant abalone,

The present invention relates to a method of producing a mutant abalone with a rapid growth rate. More specifically, the present invention relates to a method for producing mutant abalone, It relates to a method of producing abalone showing rapid growth in abalone.

Over 100 species of abalone are known all over the world and there are some abalones in Korea. The abalone species distributed in the coastal area of Korea are Haliotis gigantea), Siebold abalone (H. sieboldi), blackcurrant abalone (H. discus), ohbunjagi (Sulculcus diversicolor aquatilis), Sack ohbunjagi (Sulculus diversicolor diversicolor ), and there is H. discus hannai in the coastal area of Korea.

This abalone is attracting attention as a high-value-added fish species, and since 1976, full-scale artificial seed production has been carried out. In the method of harvesting after harvesting spoilage in the manner of aquaculture, the marine cage culture which is high in abalone survival rate and can be cultured with high density is popular.

On the other hand, in order to cope with the depletion of food resources and the increasing demand due to population increase and environmental pollution, it is essential to develop methods for improving food productivity through development of resources, improvement of seed germination and growth. In order to solve these urgent problems, various studies have been carried out. Recently, it has been approached as a hormesis study method that can induce positive effects of biological activity by proper treatment of substances classified as harmful substances. Radiation has also been reported in several crops as a physiologically active substance due to the effect of gamma-ray hormesis on germination, germination, promotion of growth and increase in yield components (Kim KJ, Choi MY, Gamma ray effect, Korean J. Environ. Biol., 25 (2): 107-114).

Radiation mutagenesis is a widely used method for onshore plants. Most seeds are seeded to irradiate gamma rays or gamma rays are irradiated to planted plants to induce mutation. In this regard, Korean Patent No. 10-1142650 discloses a method for intensively treating a new variety of berry, Rubus ruborus, using radiation, and Korean Patent No. 10-1202909 discloses a method of irradiating a new varieties of Bacillus subtilis And Korean Patent Laid-Open No. 10-2012-0069026 discloses the use of radiation for the development of a new variety of blackberry maple plants. It has also been reported that the growth of vines, the formation of tubers of potatoes, the efficiency of drug cultivation of wheat, the increase of carbohydrate content in sunflower, and the increase in photosynthesis in corn have been reported by gamma irradiation.

However, the research on hormesis by irradiation has been carried out in a lot of terrestrial organisms, and the research on marine organisms is very small. Especially, the production of mutant abalone, which has a rapid growth rate by irradiating abalone, It has not been studied.

Therefore, the present inventors developed a method for effectively inducing radiation mutation in an abalone having different mobility from plants, and at the same time, tried to produce a mutant abalone with rapid growth. As a result, in case of irradiating a gamma ray of a specific dose after inserting mature abalone into an overturned box prepared by the inventors of the present invention, it was possible to precisely irradiate the abalone, which has mobility, Sperm and oocyte were collected and the modified second generation abalone was confirmed to have a higher growth rate than the general abalone.

Korean Patent Laid-Open No. 10-2012-0128733

Accordingly, it is an object of the present invention to provide a method for effectively inducing mutation in abalone to produce mutant abalone with rapid growth.

Another object of the present invention is to provide a high growth rate abalone produced using the above method.

In order to achieve the above-mentioned object of the present invention,

(A) receiving an abalone in an abalone receiving box; (b) irradiating the box containing the rollover with radiation; (c) collecting and correcting sperm and egg in irradiated abalone; And (d) breeding the fertilized eggs by abalone.

According to an embodiment of the present invention, in the step (a), the overturning box may include an overturned inner space of a case opened at an upper side and a lower side, and a shielding film for closing or opening · Can be mounted removably.

In one embodiment of the present invention, a fixing groove for supporting the blocking film may be formed on the inner surface of the case on which the blocking film is mounted.

In one embodiment of the present invention, the case and the shielding film may be formed of a transparent material.

In an embodiment of the present invention, a sealing member made of rubber or silicone may be fitted around the outer surface of the shielding film.

In one embodiment of the present invention, a scale may be formed on the front surface of the blocking film so that the size of the handle and the rollover can be visually confirmed.

According to an embodiment of the present invention, a fitting hole is formed in the upper portion of the case and the upper portion of the shielding film, and a blocking member is mounted on the fitting hole to prevent overturning of the case to the outside of the case, May be formed obliquely.

In one embodiment of the present invention, the back surface of the case is separately formed so as to be attachable and detachable, and an irradiation through hole is formed at an interval therebetween so as to increase the irradiation dose of radiation, A rear surface coupling protrusion may be formed so as to be slidable in the vertical direction along the back side slide groove.

In one embodiment of the present invention, in the step (b), the irradiation may be performed at a dose of 10 Gy to 25 Gy using a gamma ray of ⁶⁰ Co.

The present invention also provides a high growth rate abalone produced using the above method.

The growth-promoted overturned production method according to the present invention is a method for producing an abalone in which a mature abalone is placed in an abalone holding box and irradiated with a specific dose of gamma rays to thereby effectively immobilize the abalone having mobility, Thus, the mutation induction according to the irradiation of specific dose has the advantage of producing abalone which shows a rapid growth rate compared with the abalone raised by the general aquaculture method.

1 is a schematic view schematically showing a rollover accommodating box according to the present invention.
2 is a photograph showing an actual state of the overturned box according to the present invention.
3 is a perspective view showing an overturned box according to the present invention.
4 and 5 are cross-sectional views showing another embodiment of the overturning box according to the present invention.
FIG. 6 is a perspective view showing a state of use of an overturned box according to the present invention. FIG.
FIG. 7 is a photograph of irradiating the abalone after receiving the abalone in the abalone holding box according to the present invention.
FIG. 8 is a photograph showing the size of the second generation abalone obtained after the irradiation of the irradiated abalone with the sperm and the ova taken using the vernier caliper.
FIG. 9 is a graph showing an average length of 2nd generation abalone spots according to the irradiated gamma ray dose (10 Gy, 15 Gy, 20 Gy, 25 Gy).
FIG. 10 is a photograph showing a mutant abalone that grows rapidly in a second generation abalone obtained after the radiation-irradiated spermatozoa and ova are collected.

(A) receiving an abalone in an abalone receiving box; (b) irradiating the box containing the rollover with radiation; (c) collecting and correcting sperm and egg in irradiated abalone; And (d) breeding the fertilized eggs in an abalone.

First, the step (a) of the present invention is a step of accommodating an abalone in an abalone accommodating box.

The "overturned box" of the present invention is a container for storing overturns, which means a box for accurately and effectively irradiating a live overturning fixture.

The overturning box 10 according to the present invention is roughly divided into a case 20 having an internal space and a blocking film 30 for opening or closing a surface of the case 20.

The case 20 and the shielding film 30 constituting the rollover storage box 10 may be made of a transparent or opaque material. In the present invention, the case 20 and the shielding film 30 may be formed by overturning An example in which the process is visually confirmed and formed of a transparent material so as to increase the irradiation rate of the radiation will be described.

The case 20 is formed to have a predetermined size and an inner space 21 is formed therein to accommodate one overturn and an upper surface 22 and one side surface 23 are opened to communicate with the outside .

In one embodiment of the present invention, the size of the case 20 can be of a size capable of accommodating one mature rollover, preferably 8 to 15 cm in length, 8 to 15 cm in height, and 5 to 7 cm in height More preferably 10 cm in width, 10 cm in length, and 5 cm in height.

In the present invention, the case 20 is formed by forming a plurality of frames in a rectangular box shape while the upper side 22 and the front side 23 are opened.

In addition, a fixing groove 24 is formed on the inner surface of the barrier layer 30 so as to support the barrier layer 30.

At this time, the fixing grooves 24 may be formed on the inner surface of the case 20 at intervals.

The shielding film 30 for closing or opening one side 23 of the case 20 is made of a plate having a predetermined thickness and size.

That is, the blocking layer 30 has a height corresponding to that of the fixing groove 24 formed on the open side 23 and has a height of the inner space 21.

In addition, a scale 33 is selectively formed on the front surface of the shielding film 30 so as to visually confirm the size of the grip 32 and the rollover for smooth insertion and ejection when the side surface 23 is opened or closed.

Further, it is disclosed that a sealing member 40 made of rubber or silicone can be fitted around the outer surface of the shielding film 30 so as to prevent the water and foreign substances contained therein from being discharged to the outside.

Next, the back surface 20a of the case 20 is separated and formed so as to be attachable and detachable. An irradiation through hole 20a-1 is formed in the inside of the case 20 so as to increase the irradiation amount of the radiation, 2 is formed so as to be able to slide along the rear slide groove 24a formed on the inner surface 20b of the base 20.

That is, the case 20 is configured such that a large amount of radiation can be irradiated by an overturned inside using an irradiation through hole 20a-1 formed through the back surface 20b.

At this time, the irradiation through hole 20a-1 varies depending on the size of the rollover accommodated therein, and the shape can be formed in various shapes such as a lattice.

In the upper part of the case 20 and the upper part of the shielding film 30, fitting fitting holes 25 and 35 are formed. In the fitting fitting holes 25 and 35, And the inner surface 20b of the case 20 can be formed to be inclined.

The following description will be made with reference to an embodiment of the overturning box of the present invention for irradiating the ablative radiation having the above-described configuration.

First, a transparent case 20 is formed in which a plurality of frames are formed in a rectangular box shape while an upper space 22 and a front side surface 23 are opened and an inner space 21 is formed to accommodate one overturn. .

A fixing groove 24 is formed on the same line on the inner surface of the front side surface 23 and then a blocking film 30 is detachably attached to the fixing groove 24, 10 is completed.

Next, an overturning box 10 for accommodating the overturning is received in the inner space 21 of the case 20 constituting the overturning box 10, (50).

Then, the light source body 50 is operated so that the radiation is irradiated to the overturned state accommodated in the inner space 21 of the case 20.

The inventors of the present invention fabricated an enclosure box in the following embodiment <1-1>. The embroidery enclosure box was manufactured by making an acrylic box having a size of 10 cm width, 10 cm height and 5 cm height and attaching and detaching the abalone To facilitate this, the front part was made to be detached. For this purpose, grooves were formed on both sides of the acrylic box to facilitate the removal of the front plate (see FIGS. 1 and 2).

The step (b) of the present invention is a step of irradiating a box containing an abalone, irradiating the abalone with radiation to induce a mutation.

In one embodiment of the present invention, the step (b) may include irradiating a female and a male of a mature abalone after they are housed in an abalone receiving box of the present invention, It does not remove buried water, and it can also be restricted using rubber strips to prevent movement of the abalone (see FIG. 7).

In the step (b), the irradiation may be conducted at a dose of 10 Gy to 25 Gy using a gamma ray of ⁶⁰ Co.

The step (c) of the present invention is a step of collecting and correcting sperm and egg in the abalone irradiated with radiation.

In one embodiment of the present invention, the step (c) stabilizes the abalone irradiated with the radiation for one week, then conducts an outward stimulation, irradiates ultraviolet rays, and induces scattering by performing water- Of water temperature can be corrected by applying the spermatozoa of appropriate concentration.

In the step (d) of the present invention, the embryo obtained through the step (c) is raised in an abalone.

In an embodiment of the present invention, the step (d) may be carried out in a rectangular water tank having a length of 2 m and a length of 10 m in a water temperature range of about 20 ° C to avoid the influence of the external environment on the growth of the abalone. In the case of sparrows, the scale is used as food, and the seaweed can be used as food.

Through the above steps (a) to (d), it is possible to produce a mutant abalone showing a rapid growth rate.

Therefore, the present invention provides a high growth rate abalone produced using the above method.

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are for further illustrating the present invention, and the scope of the present invention is not limited to these examples.

< Example  1>

Induce radiation mutation in abalone

<1-1> Manufacture of boxes for enclosure

An acrylic box with 10 cm width, 10 cm length and 5 cm height was prepared for stable irradiation of mobile abalone. At this time, in order to facilitate the attachment and detachment of the abalone, the front part was made to be detached. For this purpose, grooves were made on both sides of the acrylic box to facilitate the removal of the front plate. FIG. 1 is a schematic view of an overturning box according to the present invention, and FIG. 2 is a photograph taken at a front, top, side, and 45 degrees of an actually manufactured overturning box.

<1-2> Inducing Mutation of Abalone by Irradiation

Females and males of mature black abalone were purchased from Hanlim Seaweed Ranch located in Hallym, Jeju Island and irradiated with radiation by inserting one into an abalone receiving box manufactured in Example <1-1>. At this time, the water in the abalone was not removed to suppress the exposure to air, and movement was proposed using an elastic band to prevent movement of the abalone (refer to FIG. 7).

Irradiation was performed at 10, 15, 20, and 25 Gy doses of gamma rays using a cobalt (Co) -60 irradiation system of the Institute of Nuclear Science and Technology, Cheju National University. Gamma irradiation was performed at the same distance (1.5m). The irradiation time was set differently according to each irradiated dose (10 Gy: 16 min, 15 Gy: 24 min, 20 Gy: 32 min, 25 Gy: 40 min). The gamma ray dose was measured using a Fricke dosimeter.

The irradiated irradiated Abalone was prepared for 10 doses of male and 25 female for each dose. The prepared abalone was stabilized for 1 week to induce spawning, Lt; 0 &gt; C for 60 minutes) and subjected to water irradiation by ultraviolet irradiation. At this time, as control, abalone without irradiation was used, and scattering was induced by the same method as above. After the initiation of the spawning induction, an appropriate amount of sperm concentration was corrected within 1 hour at a temperature of about 20 ° C.

After the above procedure, the seran process was carried out in order to prevent polio-nucleation and deterioration of water quality by eliminating excretion secreted from the abalone or the remnant sperm. In detail, the modified eggs were taken out of the seawater and the vessel with a beaker and placed in a melena (xx13, 0.095mm in diameter 30cm), then placed in a seran vessel (30cm in diameter and 15L in diameter) It received less than 10,000. Because normal embryos sink quickly underneath, this point was identified and the upper layer of water was discarded and new seawater filled into the container. Thereafter, new sea water exchange was repeated 3 times or more.

In order to avoid the influence of the external environment on the growth of abalone, rearing was carried out in a rectangular water tank of 2 m in length and 10 m in length at a temperature range of about 20 ° C. In the case of the initial sparrow, the scale was used as food. Respectively.

< Example  2>

Spermatozoa of irradiated abalone after fertilization and fertilization. 2nd generation abalone growth measurement

In Example 1, gamma rays were irradiated on the abalone at specific doses (10, 15, 20, and 25 Gy), and then sperm and oocytes were collected and fertilized to breed second generation abalone.

In this experiment, in order to investigate the growth of second generation abalone sprouts raised through the above procedure, the 2nd generation abalone spatulas attached to the wipes raised in Example 1 were measured using vernier calipers (See FIG. 8), and the results are shown in the statistics of the size of each test. That is, a second generation abalone sprout cultivated through Example 1 was randomly extracted from 100 rabbits of each experimental group, and its size was measured and expressed as an average of ± standard deviation.

As a result, as shown in FIG. 9, the size of second generation abalone spots was measured as 5.2 ± 0.2 mm in the control (non-irradiated), whereas in the experimental group (radiation treated) (10Gy), 7.6 ± 0.6mm (15Gy), 7.3 ± 0.5mm (20Gy) and 6.9 ± 0.4mm (25Gy), respectively. It was confirmed that the 2nd generation abalone sprouting of all the experimental groups irradiated with radiation showed an excellent growth rate compared with the control. Especially, the second generation abalone spoil more than 10mm was found in 15Gy and 20Gy experimental groups.

The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

10: An acrylic box for irradiation of abalone,
20: case, 21: inner space,
22: upper portion, 23: one side surface,
24: fixing groove, 30: blocking film,
40: sealing member, 50: light source body.

Claims (10)

delete delete delete delete delete delete delete delete delete It is an overturned box designed to uniformly irradiate the abalone with radiation,
The overturning box is an inner space of an upper case and a case whose one side is opened,
A shielding film for closing or opening is detachably mounted on the one side surface,
A scale is marked on the front surface of the blocking film so that the size of the handle and the rollover can be visually confirmed,
Wherein an irradiation through-hole is formed in the case so as to increase the irradiation amount of the radiation.

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