KR101019537B1 - Methods of artificial seed production seedling for culturing urechis unicinctus - Google Patents

Abstract

The present invention relates to a method for producing seedlings for farming dogs, including breeding seedlings in a breeding facility provided outdoors.

The method for producing seedlings for farming dogs is characterized in that the hatched seedlings are raised to a certain size in indoor breeding facilities and outdoor breeding facilities after fertilization and hatching of the eggs obtained from the adult.

The production method of aquaculture dog-fired seedlings of the present invention can supply various food sources to the seedlings by breeding the young seedlings of the dog-fire in the open field, and when the seedlings are discharged to aquaculture farms, adaptability to natural environment changes and from various pathogens It has the effect of retaining immunity.

Description

Production method of aquaculture open fire seedling {METHODS OF ARTIFICIAL SEED PRODUCTION SEEDLING FOR CULTURING URECHIS UNICINCTUS}

The present invention relates to a method for producing seedlings of aquaculture aquaculture organisms, and more particularly to a method for producing seedlings for aquaculture of dogs. More particularly, the present invention relates to a method for producing seedlings of dog-fires including breeding seedlings in outdoor breeding facilities.

Open-fired production is currently rapidly declining, and resource creation by artificial seedling production is inevitable. Until now, the production of dog-fire seedlings has been carried out on a small scale indoors, and it is difficult to produce large amounts of seedlings such as industrialization due to the difficulty in supplying large quantities of food. In fact, it is difficult to find a case of selling or discharging a normal seedling of 2-4 centimeters. Relevant studies are only partly conducted on the study of poor quality selectivity by laboratory breeding (Korean Journal of Aquaculture, 1999), and the development of oocytes and larval rearing (Master's Thesis, Yeosu University, 2000).

Seeds of 3 to 4 centimeters in size must be supplied to cultivate aquatic organisms such as dogs and non-adherent shellfish such as king owl, oysters, mutants, cockles, eardrums, and shamrocks. Conventional breeding is done by spawning in early spring (around March), breeding indoors, and feeding them directly to farms. In order to produce seedlings of 3 to 4 centimeters in size by indoor breeding, it is necessary to supply a large amount of feed organisms in a timely manner, and to manage pollution so that water quality and low quality are not contaminated. In addition, since the growth of seedlings is slow indoors, it is difficult to produce high quality seedlings having a size of 3 to 4 centimeters around April to May.

The present invention has been made for the purpose of providing a method capable of producing a large amount of seedlings of aquaculture open fire. In particular, it is an object of the present invention to provide a method of producing seedlings for farming by breeding the seedlings of the dog-opening in the open field. Finally, an object of the present invention is to reduce the cost of excessively maintaining indoor breeding conditions by going through the step of breeding the seedlings of the dogs outdoors.

The present invention has been made to solve the above problems, the scattering step of obtaining eggs and sperm from the adult of the dog opening (S1); A fertilization step (S2) of fertilizing the obtained eggs to obtain fertilized eggs; A hatching step (S3) of the egg fertilized through the step (S2) and hatched to obtain larvae; The larvae hatched through the step (S3) is grown to a seedling in the indoor artificial breeding facility filled with breeding water, the dissolved oxygen concentration of the water is 7mg / l to 10mg / l, the water temperature is 20 to 25 ℃ First seedling breeding step (S4) made under the conditions; A second seedling breeding step (S5) of breeding the seedling by providing a low quality for the seedling in the indoor breeding facility filled with the breeding water for the seedling bred through the step (S4); And an outdoor breeding facility filled with the breeding water, wherein the breeding step (S6) breeds the seedlings bred through the step (S5).

In the present invention, the ceran of (S3) may use a mixed solution obtained by mixing filtered seawater and physiological saline in a ratio of 2: 1.

In the present invention, the step of obtaining the egg of (S1) is to prepare the adult dog opening in the artificial breeding facility in the breeding water filled room, so that the serotonin in the breeding water is 3 to 5ppm, hydrogen peroxide is 10 to 20ppm Dosing can be achieved by warming the water to a temperature of 5 to 7 ° C.

In addition, in the present invention, the step of obtaining the fertilized egg of the step (S2) may be made by putting the scattered eggs and sperm together in an artificial breeding facility filled with breeding water.

In the present invention, the quality of the step (S5) is preferably made of sand 100%.

In the present invention, the (S6) step of noji breeding step is preferably made in a condition that the water temperature in the artificial breeding facility provided in the outdoor is 10 ℃ to 25 ℃.

In addition, in the present invention, the (S6) step of noji breeding step is preferably a depth of 2m to 2.5m of artificial breeding ground provided outdoors.

In the present invention, the (S6) step of the noji breeding step is preferably the depth of the low quality of 1m to 1.2m.

In the present invention, the (S1) step of the scattering is preferably made in November, the (S6) step of noji breeding step is the daily average water temperature in the indoor breeding facility is 10 ℃ to 25 ℃, March to 6 It is preferred to be carried out on a monthly basis.

Finally, in the present invention, before performing the step (S1), blocking the light to the adult of the dog-opening, exposing the adult to the light blocking the light for 30 minutes to 1 hour before scattering It is preferable to further include.

The production method of aquaculture open fire seedling of this invention is effective in supplying a large quantity of aquaculture open fire seedling. In particular, it is possible to supply various food sources to the seedlings by going through the stages of breeding the seedlings in the open field, and when the seedlings are discharged to the farms in the future, they have the effect of retaining the adaptability to natural environment changes and immunity from various pathogens. have. In particular, the production method of the present invention has the effect of reducing the cost excessively required to maintain indoor breeding conditions by breeding the seedlings of dog-opening outdoors.

The production method of aquaculture open fire seedling by this invention provides the method which can produce a large quantity of seedlings of aquaculture open fire. In particular, the present invention provides a seedling production method for providing a high quality seedlings that can be effectively adapted to the surrounding environment when breeding the dog seedlings in a kennel provided in the outdoors to discharge them to a farm. Finally, the present invention provides a seedling production method that can reduce the excessive cost of maintaining the indoor breeding conditions by breeding the seedlings of the open dog outdoors.

Definition of Terms

1. 'Low quality' is, in principle, used as a major habitat for benthic creatures that infiltrate and settle in sedimented or deposited material on the bottom of lakes or seas. The low quality environment can be artificially provided in the artificial breeding facility, and 'low quality' as used herein means artificially prepared in the artificial breeding ground for breeding of seedlings.

2. 'Floating larvae' refers to larvae that live in water after hatching and have a size of about 300㎛.

3. 'Sapling' refers to a state in which growth is possible even when discharged to the farm, and in the present invention, it means a young dog seedling which is 1 to 2 mm or more after growing in the floating larvae.

4. 'Infiltrate' means that this young dog-breed seedling dwells in inferior quality.

5. 'No-land breeding' means breeding young dog-fired seedlings using artificial breeding facilities of seedlings set up outdoors.

6. 'Breeding water' is seawater or liquid with the same composition as seawater.

It is meant to be provided as a place for habitation of sieves.

Detailed description of the invention

The method for producing seedlings for aquaculture according to the present invention is a scattering step (S1) of obtaining eggs and sperm from the adult of the dogbowl, a fertilization step (S2) to obtain a fertilized egg by modifying the obtained eggs (S2) The eggs were bred in a mixed solution of filtered seawater and physiological saline, and hatched to obtain larvae (S3), the larvae hatched through the step (S3) are bred in the indoor artificial breeding facility filled with breeding water The first seedling breeding step (S4) to grow into a seedling (S4), the second seedling breeding to breed the seedlings by providing a low quality for infiltrating the young dog fire seedlings in the indoor breeding facility filled with the number of breeding seedlings through the step (S4) It is characterized in that it comprises a step (S5) and an outdoor breeding facility filled with breeding water, wherein the outland breeding step (S6) for breeding the seedlings bred through the step (S5).

In addition, before performing the step (S1), it is preferable to further block the exposure of the adult of the dog opening to light and to expose the adult to light for 30 minutes to 1 hour before scattering. .

Hereinafter, the method for producing seedlings of the aquaculture open fire will be described in detail for each step.

(1) spawning stage

The spawning stage is the process of obtaining eggs and sperm from an adult adult. This step is carried out in an indoor artificial breeding facility. The artificial breeding facility may be made of synthetic resin, glass or concrete material. In order to stimulate the spawning of the adult by changing the water temperature, it is preferable to gradually warm the water temperature of the breeding water in which the adult is incubated at about 5 to 7 ° C. More preferably, after maintaining the water temperature of 12 to 15 ℃ for breeding the adult to gradually warm the breeding water so that the water temperature is 17 to 22 ℃ from 5 to 10 hours before the start of the spawning.

In addition, in order to stimulate the scattering of the adult, it is preferable to administer 10-20 ml of hydrogen peroxide and 3-5 ml of serotonin per 1 liter of seawater. It is effective to obtain more than twice the quality of fertilized eggs, but the amount of eggs varies depending on the adult, but during the above treatment, large-scale spawning is possible. have.

For more effective scattering, preferably, additionally performing a step of shielding the adult from scattering to light (natural light level illumination) for 30 to 60 minutes after blocking the light at the breeding facility so that the adult is not exposed to light. It is desirable to.

(2) modification

The fertilization is a step of obtaining a fertilized egg, by putting together the eggs and sperm obtained from the adult in the artificial breeding facility filled with breeding water. The fertilization takes about 10 to 20 minutes after the eggs and sperm are put into artificial breeding facilities. For the correction, the number of sperm is preferably 90,000 to 100,000 / sperm is injected. In addition, it is preferable to perform aeration in the breeding facility where the correction is made so that oxygen is sufficiently supplied.

(3) hatching stage

The hatching step is to hatch the fertilized egg to obtain larvae. This step is preferably done in an indoor artificial breeding facility filled with breeding water. When the fertilized egg is obtained in the fertilization step, the fertilized egg is collected by a net to remove foreign substances and surplus sperm. Since the fertilized egg is about 160 μm on average, or stretchable, it is preferable to prevent the fertilized egg from escaping by using a muller network of 50 to 70 μm to remove the foreign matter. When the fertilized egg is lumped or clustered, the fertilization rate is extremely bad, so that the slime is dissolved by washing with a mixed solution of 2: 1 filtered seawater and physiological saline. At the end of the seran, the fertilized egg is allowed to hatch. In the incubation step, the density of the fertilized egg in the artificial breeding facility is preferably maintained at 20 individuals / ml or less. In addition, it is preferable to maintain the water temperature of 20 to 25 ℃ artificial breeding facility in which the fertilized egg is added for hatching. Hatching of the fertilized egg is preferably made in an artificial breeding facility with a wide bottom and a shallow depth. The fertilized egg hatches after 25 to 30 hours.

(4) the first seedling breeding stage

The first seedling breeding step is a step in which the larvae hatched from the fertilized eggs grow into young dog seedlings, and the breeding step is performed until the seedlings infiltrate the poor quality. In order to breed the hatched larvae, an artificial breeding facility filled with breeding water indoors is prepared, and the larvae are injected. It is preferable that the habitat density of the larvae injected into the artificial breeding facility is 4 to 5 / ml. If the amount exceeds 5 / ml, the high density of the habitat may adversely affect the growth.

The artificial breeding facility supplies sufficient oxygen using airstones, and the breeding water is used by filtering seawater with a filter having a level of several microns. In order to prevent contamination of the water quality of the kennel due to the administration of viscous substances and feed organisms discharged from the seedlings grown in the larvae and larvae, it is preferable to return the breeding water and increase the dissolved oxygen concentration to prevent the death of fertilized eggs and larvae. . The breeding water is preferably returned to the whole amount every day and maintain the dissolved oxygen concentration of 7mg / l to 10mg / l. In order to maintain the dissolved oxygen concentration at 7 mg / L to 10 mg / L, it is preferable to use a liquid oxygen injection method rather than the conventional aeration method.

Moreover, it is preferable that the temperature of the said breeding water is 20-25 degreeC. When the temperature of the breeding water is lower than 20 ° C or higher than 25 ° C, the growth of the larvae is lowered.

Feeding organisms feed from 18,000 to 20,000 cell / ml · day until 1-segment period after hatching, and 25,000 to 30,000 cell / ml · day when the segment starts to form. In the food organisms are holding Chitose Los calcineurin trans (Chaetoceros calcitrans), isopropyl Cri cis galvanic or (Isochrysis galbana), Pavlova ruteri (Pavlova utheri), par five dark tea room tree cor nutum (Phaeodactylum tricornutum), tetra-cell Miss (Tetraselmis sp .), knitted together's teeth (Nitzschia sp.), preferred that a mixture of the class or the like, which may have a fast effect growth of larvae compared to the single class.

The opening of the dog is 2 or 3 days after hatching, 1 season, 7 days after the seven seasons, 15 to 17 days after the 13 seasons to start infiltrating. When the fertilized eggs of the dog embryos hatched in November, the seawater temperature of the kennel is maintained at 20 to 25 ° C, and if enough feed organisms are supplied, they grow into seedlings in mid-December after hatching and start infiltrating.

(5) second seedling breeding stage

The young dog-fire seedlings established in the first seedling breeding step are infiltrated by the submerged substrate at the bottom of the seawater and inhabited. In order to infiltrate the seedling, an artificial kennel made of a material of synthetic resin is prepared, and a submerged substrate (low quality) is provided at the bottom of the kennel. The immersion substrate is one having 80 to 100% sand, preferably 90 to 100% and most preferably 100% sand. More than 80% of the infiltrating substrates are made of sand, which is good for drainage, so that waste products can be discharged quickly, the growth process can be easily observed, and it is easy to manage and move. In the case of the saniparum mixed with sand and sand as the infiltrating substrate, water is poor, and harmful substances such as hydrogen sulfide and ammonia are generated due to decay of waste products such as food or bunny, and in low quality, loss of ignition loss, low COD, Increased sulfide (AVS, Acid Volatile sulfite) worsens the breeding environment. In addition, if the low quality environment worsens, in addition to direct mortality, the ability to eat is reduced, thereby slowing the growth of the larvae (see Example 2). Therefore, it is preferable to spray only a small amount of fine bouney only at the beginning of the seeding of the seedling and to manage in an artificial kennel made of 80% or more, preferably 90% or more, most preferably 100% sand until the end of the seedling production. Seawater filtered with a micron-meter filter is used as breeding water in an artificial kennel provided with the steep substrate. The seawater uses filtered seawater warmed to 10 to 15 ℃ and performs aeration and flowing water to prevent contamination. As food for the infiltration group seed organism holding Chitose Los calcineurin trans (Chaetoceros calcitrans), isopropyl Cri cis galvanic or (Isochrysis galbana), Pavlova ruteri (Pavlova utheri), par five dark tea room tree cor nutum (Phaeodactylum tricornutum), tetra-cell Ms (Tetraselmis sp.), a mixture knit's teeth (Nitzschia sp.), etc. the class is an approximately 30,000 to 40,000 cell / ㎖ 1 day.

(6) outland breeding stage

Open-field breeding refers to the raising of seedlings in an artificial breeding ground set up outdoors. The outland breeding step refers to a step of growing a seedling grown to a size of 1 to 2 centimeters through a second seedling breeding step in a breeding farm provided outdoors to provide a breeding seedling to grow to an appropriate size.

When the floating larvae hatch in November, they sneak in mid-December and grow to 2-4 mm in January, 4-6 mm in February, and 1-2 cm in March. It is suitable for open-field breeding from March to June.

When breeding only in the open field from the beginning of hatching of the seedlings, since the growth is slower than breeding indoors, it is preferable that the breeding is performed at the indoor breeding ground initially to grow to 1 to 2 centimeters. Seedlings of the second seedling breeding step to raise and raise an artificial breeding ground outdoors when the water temperature rises by 10 ° C or more. The artificial kennel is preferably made in a kennel artificially provided outdoors.

The artificial kennel may use a method of arranging a water tank made of glass, synthetic resin or cement, or digging a pit in the ground and filling a breeding water to prepare a habitat. In particular, the artificial kennel is easy to maintain the water temperature and water quality management by digging the ground to build a dike and to fill the breeding water there and breeding on a large scale.

In addition, the outdoor kennel utilizes a low quality material made of 80% or more, preferably 90% or more, and most preferably 100% sand as a submerged substrate. Breeding water flows more than 20 hours, and 50% of water is returned a day. It is desirable to maintain a depth of 2 m or more in order to prevent the rapid rise and fall of water temperature during the breeding.

Since the breeding of the open field is highly affected by temperature, the water temperature, pH, salinity, dissolved oxygen, alkalinity, etc. are severely changed, so the dog breeding seedlings are transferred to the open field, and the artificial kennel is covered with a heat insulating cover of vinyl, etc. Maintain water temperature.

The appropriate water temperature of the noji breeding step is 10 ° C to 20 ° C, more preferably 15 ° C to 20 ° C. Most preferably, it is 15-17 degreeC. If the water temperature is lower than 10 ℃ or higher than 20 ℃, the growth rate is rapidly lowered or stopped. If it exceeds 20 ℃, growth is slow up to 30 ℃, especially if it exceeds 32 ℃, the body is filled with revenge, floating on water and unable to infiltrate and die. In order to prevent the death or growth delay caused by high water temperature, infiltrate the sand more than 1m and control the water temperature below 30 ℃ by using a cooler or low ground water. The water temperature is good for growth from April to June, but the water temperature often exceeds 30 ℃ from July. Therefore, seedling production in the open field should be completed by at least June.

Before the seedlings raised indoors are transferred to the open field, feed organisms and PSB (photosynthetic bacteria) are supplied in advance, and breeding water for the seedlings is prepared in advance to prevent death due to rapid water quality change.

In the food organisms are holding Chitose Los calcineurin trans (Chaetoceros calcitrans), isopropyl Cri cis galvanic or (Isochrysis galbana), Pavlova ruteri (Pavlova utheri), par five dark tea room tree cor nutum (Phaeodactylum tricornutum), tetra-cell Miss (Tetraselmis sp .), knitted's teeth (and mixed by supplying the like Nitzschia sp.), is supplied as a powder seaweed (kelp wheat) such as it grows.

Kinds of photosynthetic bacteria include red bacterium bacterium, green bacterium chlorodium (Chlorobium), chromatium (Chromatium), non-yellow bacterium Rhodospirillum (Rhodospirillum), Rhodobacter (Rhodobacter) and the like. Photosynthetic bacteria are anaerobic bacteria that make sugar by photosynthesis. Bacterial red bacteria and green bacterium have bacteriochlorophyll, and photosynthesis is made of CO ₂ and hydrogen compounds (H ₂ S). Photosynthetic bacteria contain large amounts of carotinoids, which are effective in supplying organic carbon because they have photosynthetic capacity. In addition, it is able to supply organic nitrogen with excellent nitrogen fixing ability, and it is fed by secreting amino acids (proline, etc.), nucleic acids (uracil, guanine, etc.), vitamins (biotin, etc.) It promotes the growth of living things. In addition, it removes harmful substances by decomposing organic substances without consuming oxygen, and converting them into amino acids, nucleic acids, etc. using gaseous substances such as ammonia, hydrogen sulfide, putrescine, methyl mercapdan, and amines as nutrients. It is known to have an excellent effect of removing harmful substances. In addition, it produces antibacterial and antiviral substances, inhibits the growth of harmful bacteria, facilitates water quality management of the open field, and improves the microorganisms in the water by creating alkaline conditions to improve the growth of beneficial bacteria. It acts to stabilize water quality.

The food organism of the seedling supplies 10㎥ / day based on the area of 660㎡ of artificial breeding ground provided in the open field. The feeding of the feed organisms is preferably made by transporting the feed organisms to the open field in a culture tank for culturing a large amount.

In the seedling, the resistance to salinity is up to 36 ‰, and survival is possible even at low salinity of about 10 ‰. Dissolved oxygen can survive at low concentrations of 3 mg / l, so a small amount of aeration can be used for breeding.

In most cases, the alkalinity is less than 50 mg / l, indicating a poor buffering capacity, which is the biggest cause of the temporary killing of plankton or food organisms. Therefore, it is important to maintain proper alkalinity for stable open-air environment, and to improve alkalinity of water to 100mg / l or more by spraying around 100 to 150g of calcium carbonate ground oyster shell for alkalinity composition. It reacts with hydrogen sulfide to insolubilize it with harmless CaS (calcium sulfide), which helps to improve quality. Since alkalinity is partially made in the case of noji, maintaining the alkalinity at 80 to 100 mg / l does not cause water change.

In the production of seedlings for aquaculture open fire, there are the following advantages by introducing the open field breeding step. In the case of carrying out the open field breeding step in April, since the water temperature rises rapidly as the spring temperature increases, the seedling has an advantage of being able to quickly enter the growing temperature zone. In addition, by securing a wider space than the indoor artificial breeding ground, it is possible to prevent a sudden change in water temperature, which enables stable management of seedling breeding.

In terms of supply of food organisms, the food organisms multiply themselves by water stability and sunlight through photosynthetic bacteria, thereby reducing the supply of food organisms. In addition, the natural ecological environment is built in the kennels in the open field, so that various natural food organisms such as microalgae, algae, colorless bacteria, coarse algae, rotifers, etc. can be reproduced. In terms of pollution of seawater injected into the kennel, waste, dissolved inorganic nitrogen and phosphate are absorbed by nitrifying bacteria, photosynthetic bacteria, and algae (brown seaweed, etc.), preventing water pollution and eliminating the need for a separate circulating filtration facility. have.

Production costs in the field are reduced in production costs except for the initial facility cost, which can improve the added value of seedling production, and can accommodate more than twice as much as indoor breeding. It is essential.

The production method of the aquaculture dog-fired seedling of the present invention is suitable for the production of aquaculture seedlings of non-adherent shellfish, king clam, key clam, remodeling, cockle, tympanic membrane or kalamak in addition to the dog-fire.

Example 1 Production of Seedlings (November Scattering)

(1) scattering and fertilization steps

In November, the adult adult dogfire was secured and housed in a 100-liter tank that blocked light. Light was added to the tank for 1 hour, 50 ml of hydrogen peroxide and 20 ml of serotonin were injected at 3% concentration, and the temperature of the breeding water of the tank was warmed to 20 ° C. and left to stand for 3 hours to spawn. The eggs and sperm were mixed and placed for 30 minutes to obtain fertilized eggs. After fertilization, foreign bodies and surplus sperm were removed from fertilized eggs using a muller network of 50 μm mesh and washed with 5% physiological saline. The fertilized eggs were housed in a plastic container with a large bottom and allowed to hatch to obtain larvae of open fire.

(2) the first seedling breeding stage

The hatched larvae were introduced into a plastic tank (100 liters) indoors and were reared (average breeding density of 5 / ml). The tank was injected with breeding water filtered by a 1 μm sized cartridge filter. In the food organisms are holding Chitose Los calcineurin trans (Chaetoceros calcitrans), isopropyl Cri cis galvanic or (Isochrysis galbana), Pavlova ruteri (Pavlova utheri), par five dark tea room tree cor nutum (Phaeodactylum tricornutum), tetra-cell Miss (Tetraselmis sp .), a mixture knit's teeth (Nitzschia sp.), etc. were the class. Feeding amount was 18,000 to 20,000 cell / ㎖ · day until the 1-sieger season after hatching, 25,000 to 30,000 cell / ㎖ · day when the segment began to occur. Liquid oxygen was injected into the tank to maintain the dissolved oxygen concentration in the breeding water at 7 mg / L to 10 mg / L, and the breeding water temperature of the artificial tank was maintained at 20 to 23 ° C.

(3) the second seedling breeding stage

The hatched larvae were put in a plastic tank (100 liters) indoors and were raised to a size of 1 to 2 centimeters (average breeding density of 5 / ml). A bottom surface of the tank was provided with a immersion depth of 50 cm 3, and the immersion quality was 100% sand. The tank was injected with breeding water filtered with a 1 μm sized cartridge filter. In order to prevent contamination of the breeding water, aeration and flowing water were performed, and the temperature of the breeding water was maintained at 10 to 15 ° C. As food for the larvae organisms holding Chitose Los calcineurin trans (Chaetoceros calcitrans), isopropyl Cri cis galvanic or (Isochrysis galbana), Pavlova ruteri (Pavlova utheri), par five dark tea room tree cor nutum (Phaeodactylum tricornutum), tetra-cell miss ( Tetraselmis sp.), knitted's teeth (Nitzschia sp.), etc. were mixed to a class of 30,000 to about 40,000 cell / ㎖ 1 day.

(4) outland breeding stage

An artificial breeding facility was set up outdoors and 100% sand was added to it to fill the breeding water. The depth of the sand was 1 meter and the water depth was 2 m. Dog breeding seedlings grown to 1 to 2 centimeters in size were placed in the breeding facility. Plastic cover was used to prevent the water temperature of the breeding water filled in the artificial tank is less than 10 ℃. In the food organisms are holding Chitose Los calcineurin trans (Chaetoceros calcitrans), isopropyl Cri cis galvanic or (Isochrysis galbana), Pavlova ruteri (Pavlova utheri), par five dark tea room tree cor nutum (Phaeodactylum tricornutum), tetra-cell Miss (Tetraselmis sp .), knitted's teeth (and mixed by supplying the like Nitzschia sp.), was supplied as a powder seaweed (kelp wheat) such as it grows. Through the above method, an average of 5 centimeters in size dog breeding breeding was obtained.

Comparative Example 1. Production of seedlings (opening in March)

The same method as in Example 1, except that the dogling larvae spawned in March were used.

Open fire seedlings were produced.

Table 1 below summarizes the growth of the early seedlings laid in November and the spring seedlings laid in March. As summarized in Table 1 below, the hatched larvae hatched in November were sufficiently grown to about 2 centimeters through indoor breeding, so that they could be produced to a size suitable for aquaculture seedlings in the after-farming stage. However, in the case of open larvae hatched in March, it was difficult to produce high quality seedlings even if the seedlings were about 1 centimeter in size when they were grown afterwards.

Comparison of Growth Rate between Early Opening Seedlings and Spring Seedlings division
January February In March April (outdoor) May (outdoor) June (outdoor) Remarks
size
(Mm) weight
(g) size
(Mm) weight
(g) size
(Mm) weight
(g) size
(Mm) weight
(g) size
(Mm) weight
(g) size
(Mm) weight
(g) Early
Seedling 3.2 - 5.4 0.01 11.8 0.08 32.5 0.25 38.8 1.65 51.3 3.27 November
hatching Spring
Seedling
- - 3.8 - 10.3 0.07 18.3 0.18 32.6 1.20 In March
hatching

 Example 2 Pollutant Test Results of Saani and Sand (100% Sand) as Substrate Substrate

Water content, ignition loss, low COD, and sulfide of each infiltrating substrate were analyzed to determine the degree of contamination of the infiltrating substrate consisting of sand (100% sand) and sani quality (sand to sand ratio 1: 1). The experimental results are summarized in Table 2 below. The moisture content of both submerged substrates was not significantly different, but the loss of thermal loss resulted in less organic matter and thus less pollution. All of the low CODs were below the standard, but in the sands, they were found to be less than ₁ mgO ₂ /g.dry, indicating very low contamination. The results of the analysis of sulfides showed very low sand quality, while the sani quality ranged from 1.3 to 2.2 mgS / g.dry, exceeding the standard value in one test group and approaching the standard value in two test groups. The environment was much lower than that of sand. Table 2 summarizes the results of the analysis of sani quality and sand quality.

Results of poor quality analysis according to the components of the infiltrating substrate Survey Item
division Moisture content (%) Ignition loss (%) Low quality COD
(MgO ₂ /g.dry) sulfide
(MgS / g.dry) Remarks Saanil 1 25.07 3.15 6.06 0.181 Sand: 뻘 = 1: 1 Saniji 2 26.41 3.32 5.70 0.220 Sand: 뻘 = 1: 1 Sanijiil 3 27.91 3.05 6.57 0.132 Sand: 뻘 = 1: 1 Vagina 24.81 1.01 0.95 0.022 Sand: 뻘 = 1: 1 Reference value none 7 or less (foreign) 20 or less (Japan) 0.2 or less (Japan) 100% sand

* According to the 2005 Marine Pollution Process Test Method (Ministry of Maritime Affairs and Fisheries)

1 is a flowchart showing the seedling production method of aquaculture open fire of the present invention in order.

Figure 2 is a photograph showing a state of scattering dog opening.

3 is a photograph showing a state of washing the fertilized egg.

4 and 5 are photographs showing examples of artificial breeding facilities provided indoors for the first and second seedling breeding stages.

Figure 6 shows an example of an artificial breeding facility provided outdoors for the off-land breeding step.

Figure 7 shows the dog grows to the size of the nursery seedlings.

Claims (11)

In the production of the seedling for the aquaculture of the open fire, (S1) spawning step of obtaining eggs and sperm from the adult of the dog opening; (S2) a fertilization step of fertilizing the obtained eggs to obtain fertilized eggs; (S3) the hatching step of serene the fertilized egg through the step (S2), hatching to obtain larvae; (S4) the larvae hatched through the step (S3) is grown to a seedling in the indoor artificial breeding facility filled with breeding water, the dissolved oxygen concentration of the water is 7mg / l to 10mg / l, the water temperature is 20 to A first seedling breeding step made at 25 ° C .; (S5) a second seedling breeding step of breeding seedlings by providing a low quality for the seedlings in the indoor breeding facility filled with the breeding water for the seedlings bred through the step (S4); And (S6) an open-air breeding facility in which the breeding water is filled, and the outland breeding step of breeding the seedlings bred through the step (S5); Production method of aquaculture open fire seedling comprising a. The method of claim 1, The seran of (S3) is a method of producing a breeding dog breeding seedling, characterized in that using a mixed solution of filtered seawater and physiological saline in a ratio of 2: 1. The method of claim 1, The step of obtaining the eggs of (S1) is to prepare the adult in the dog breeding facility in the indoor artificial breeding facility filled with breeding water, serotonin in the breeding water is administered to 3 to 5ppm, hydrogen peroxide to 10 to 20ppm, the water of The production method of aquaculture open fire seedling characterized by heating so that temperature may become 5-7 degreeC. The method of claim 1, The step of obtaining the fertilized egg of the step (S2) is a method of producing a breeding dog breeding seedling, characterized in that the egg and the sperm to the artificial breeding facility filled with breeding water. The method of claim 1, The low quality of the step (S5) is a production method of a breeding dog breeding seedling, characterized in that made of 100% sand. The method of claim 1, The (S6) step of noji breeding step is a production method of a breeding dog breeding seedling, characterized in that the water temperature in the artificial breeding facility provided in the outdoor is made under the condition of 10 ℃ to 25 ℃. The method of claim 1, The (S6) step of the open field breeding step is a method of producing a breeding dog breeding seedling, characterized in that the depth of the artificial kennel provided outdoors is 2m to 2.5m. The method of claim 1, The (S6) step of the noji breeding step is a production method of a breeding dog breeding seedling, characterized in that the depth of the low quality is 1m to 1.2m. The method of claim 1, The method of producing a breeding dog seedlings, characterized in that the scattering in the step (S1) is made in November. The method of claim 1 The (S6) step of the open field breeding step is a method of producing a breeding dog breeding seedling, characterized in that the daily average water temperature in the indoor breeding facility is 10 ℃ to 25 ℃, carried out in March to June. The method of claim 1, The method further comprises the step of blocking the light to the adult of the dog-opening before performing the step (S1), and exposing the adult to the light for 30 minutes to 1 hour before scattering to the light (light) Production method of dragon dog seedlings.

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