KR101346281B1 - Compositions for preventing, improving or treating gout, containing extracts of twenty nine medicine plants as active ingredient - Google Patents

Abstract

The present invention relates to compositions for preventing, improving, or treating gout, containing extracts of twenty nine medicine plants as an active ingredient. More particularly, the present invention relates to compositions for preventing, improving, or treating gout, the compositions containing, as an active ingredient, extracts of gold, Lonicera japonica, Actinidiapolygama, myrrh, Siegesbeckia, Chaenomeles sinensis, Angelica dahurica root, Schizonepetae Spica, poncirus, kalopanx, taraxacum herb, Clematis florida, Bambusae caulis, corydaline, safflower, cnidium, achyranthes, Atractylodes macrocephala, dried orange peel, white atractylis, forsythia fruit, Rehmannia glutinosa, Poria cocos Wolf, licorice, platycodon, Korean aralia root, coix, Sinapis alba, and Allium fistulosum. According to the present invention, the extracts of twenty nine medicine plants contain a large amount of phenolic compounds, have high ability to remove DPPH radicals and ABTS radicals, and exhibit excellent activity to inhibit xanthine oxidase, and thus are useful for prevention, improvement, or treatment of gout. Further, the extracts of the present invention have edible medicine plants as raw material, and thus have few side effects. Especially, the compositions can be very easily taken when being prepared in a type of functional food or food additive, and thus the compositions of the present invention are expected to be very effective.

Description

Compositions for preventing, improving or treating gout, containing 29 medicinal extracts as active ingredients {compositions for preventing, improving or treating gout, containing extracts of twenty nine medicine plants as active ingredient}

The present invention relates to a composition for preventing, improving or treating gout containing 29 medicinal herb extracts as an active ingredient, specifically, golden, gold, silver, myrrh, myrrh, euphoria, tree, white paper, mold opening, jisil, Haedongpi, pogongyoung, Gastrointestinal tract, kill, Hyunho colors, safflower, celestial organs, dew, creation, dermis, baekrye, yeonkyo, geonjihwang, baekbokyeong, licorice, gilyeong, venom, uiyiin, white ginseng and ginseng extracts as an active ingredient to prevent, improve or treat It relates to a composition for.

Gout is an abnormality in purine metabolism (1), which leads to extreme pain, fever, and swelling due to the increase in serum uric acid concentrations and the deposition of urate crystals in the blood in the cartilage of the joints and surrounding tissues. If uric acid concentration is 7.0 mg / dL or higher (6.0 mg / dL in females), it is defined as hyperuricemia, and it is determined that hyperuricemia alone does not produce gout symptoms but is directly related (3). It occurs mainly in men, rather than women, because men's ability to remove uric acid in the kidneys decreases with age (4).

According to the statistics of patients treated in hospital for gout disease, inpatient treatment increased by 10.4% to 5,111 in 2009 and 5,643 in 2010. Cases increased by 8% to 222,495 in 2009 and 240,203 in 2010. The overall gout patients increased by 8% to 227,606 in 2009 and 245,846 in 2010 (5 ~ 6).

When examining gout paths through the organs of the body, the kidneys are the source of fluid metabolism in the body. When the kidneys are deteriorated due to various causes such as stress, excessive drinking, and overdose of drugs, (精 氣) is weakened to support the body (氣) is united, phlegm and fish blood (瘀血) is generated and accumulate in the blood, which causes uric acid excretion dysfunction, and hyperuricemia causes gout It can be said that. In general, gout patients are known to have 20 to 60% more kidney disease than the general population (7).

Gout can be divided into acute and chronic gout, which starts with mild symptoms from the beginning and usually causes severe pain and swelling with heat on both toes (8-11). Chronic nodular gout and gouty nephropathy, which can lead to white toothpaste-like nodules due to various factors such as drinking, intense exercise, dehydration, surgery, bleeding, trauma, and excessive drug use affecting uric acid discharge As a result, the joints lose their function and even malformations occur (12).

It has been reported that side effects caused by drugs such as colchicine, nonsteroidal anti-inflammatory drugs, and steroids, which are used for gout patients with severe pain, may suffer from complications such as metabolic syndrome and kidney disease and shorten their lifespan (13). . Therefore, while developing safe and accurate medicines, there are no results.

In recent years, the increase in meat consumption, obesity increase, lack of exercise, and drinking culture caused by various Korean drinking cultures have become the main causes of gout. It is well known that proper medication and diet are the most important for prevention, and that moderate exercise such as proper exercise, weight control, and abstinence are necessary.

Some herbs are known to be effective against gout, and some studies have suggested that some of them may be effective in treating and preventing gout (14–16).

However, it is very difficult to treat gout with one herb, and it is still difficult to treat and improve gout by suppressing the generation of hyperuric acid that causes severe pain of gout and restoring kidney function to normal. Or food development is still slow.

Accordingly, the present inventors have attempted to develop a medicine that is effective in gout and has fewer side effects, and also made an intensive research effort to be able to apply it in the form of food which can be easily consumed in daily life.

First of all, we studied herbal medicines and edible herbs in consideration of side effects, and among these herbs, we eliminated the extreme pain of gout symptoms, destroyed the clumps, phlegm, and blood, eliminated inflammation and poisoning, and lowered the heat in the pain area and urine. Herbs that were able to make the whole meridians smooth through the excretion function were selected and combined, and the effects on gout were verified through various experiments.

As a result, 29 kinds of edible herb extracts are excellent in preventing, delaying, improving or treating gout, and because they use edible herbs as raw materials, they have less side effects and can be easily applied to foods. After confirming that it can be easily taken for a long time without completing the present invention.

Lee, Gwi-Nyung and Jong-Soon Lee: Clinical Pathology, Seoul, Korea. 86-88, 92-98, 683-685, 732, 733, 749-745, 752, 755, 756, 1404-1406, 1993. 2. Lee YH. Diagnosis and management of gout. 2002. J Korean Acad Fam Med 23: pp. 261-266. 3. Janson RW. 2002. Gout. In Rheumatology Secrets: West S, ed. Hanley & Belfus Inc., Philadelphia, USA. pp. 325-333. 4. Health Insurance Review & Assessment Service. 2011. National Health Insurance Statistical Yearbook. pp. 559-578. 5. 2010 Annual Health Insurance Statistics Annual Report (December 2011) Korea National Statistical Office No. 920-06 ISSN 1738-8945 Status of hospitalization by hospital subclasses 559-578. 6. 2011 Annual Report of Health Insurance Statistics (December 2012) Approved by National Statistical Office No. 92006 ISSN 1738-8945 Status of hospitalization by hospital subclasses 624-641. Kutzing MK, Firestein BL. Altered uric acid levels and disease states. J Pharmacol Exp Ther 2008; 324: pp. 1-7. Domonks, A. N., Arnold, H. L & Odom, R. B .: Errors of Metabolism, Diseases of the skin, 7th Ed., W.B. Saunders, pp. 684-685, 1982. 9. Wyngaarden, J. B .: Gout in Textbook of Medicine Wyngaarden, J. B., L. H., 16TH eD., W. B. Saunders, pp. 1107-1118, 1982. 10. 李 文 李 外: Journal of the Institute, Seoul, Geumgang Publishing Co., pp. 2304-2317. 11. Korean Dermatological Association: Dermatology, Seoul, Eommungak, pp. 417-418. 12. The Korean Journal of Rheumatology (So-Young Cho, Yong-Bum Park, Chan-Hee Lee) Vol. 18, No. 1, March, 2011, p. 26. 13. The Korean Journal of Rheumatology Vol. 18, No. 4. Rising Gout, Life Threatening Public Enemy, Jung Soo Song, 2011, pp. 234-238. 14. The Institute of Oriental Medicine, Daejeon University: The Effect of Yindong on Gout of Rats induced by Monosodium Urate, Vol. 17, No. 1, pp. 83-92, 2008. 15. The Research Institute of Oriental Medicine, Daejeon University.The Effect of Smilacis Glabrae Rhizoma on Rsts with Gout induced by Monosodium Urate, Vol. 17, No. 1, pp. 95-103, 2008. 16 Kang, M. G., Bolormaa, Zanabaatar., Lee, J. S., Sea, G. S., Lee, J. S., Antithy pertensive Activity and Anti-gout Activity of Mushroom Sarcodon aspratus, Kor. j. Mycol. 39 (1): pp. 53-56 (2011).

Therefore, the main object of the present invention is to provide a pharmaceutical composition, nutraceutical or food additive with excellent side effects of preventing, delaying, improving or treating gout and having fewer side effects.

According to an aspect of the present invention, the present invention is golden, gold, silver, myrrh, myrrh, bliss, neck, white paper, mold opening, fruiting, haedongpi, pogongyeong, lieutenant line, kill, hyundai, safflower, celestial arch, dew, creation, dermis, Provided is a pharmaceutical composition for preventing or treating gout containing water extracts of Baekchul, Yeongyo, Guernsey, Baekbokyeong, Licorice, Gilgyeong, Dokyeong, Uiin, Baekja and Chongbaek as active ingredients.

According to another aspect of the present invention, the present invention provides a functional food for preventing or improving gout containing the water extract as an active ingredient.

According to another aspect of the present invention, the present invention provides a food additive for preventing or improving gout containing the water extract as an active ingredient.

The present invention is based on a variety of herbal medicines known to be used for a specific purpose or effective for a particular symptom, and the present inventors select and combine the medicines determined to be necessary for the prevention, improvement or treatment effect of gout, It is completed through proof.

According to the existing research results on gout, the present inventors ① destroy the inflammation of uric acid, ② eliminate fever and pain, ③ communicate with meridians, ④ remove fish blood, ⑤ smooth the excretion function such as urine, and ⑥ watch the kidneys. It was determined that gout can be prevented and treated as long as the function can be restored to normal, and thus, the combination of medicines was determined. The ratio of each medicine was also determined in consideration of the necessary effects.

Mokcheonryo, Myrrh, White Paper, Hyunho Color, Baekjaja, and Maengnseon for Jitong, Hyeomyeong, Thirsty and Thawing Blood For safflower, saenggung, cheongung, gilgyeong, and hyssop for live bloodfish, hyeonggae, geonjihwang, venom and baekbaek for haemul, for the digging, Creation, harvest and licorice are organized for the sake of view, dermis for selfishness, and Baekbokryeong and Uiin for the sake of water.

In the present invention, 29 kinds of medicines are used, which are defined as follows on the basis of those listed in the Pharmacopoeia for each medicine.

Golden (黄 芩) is the root of the decayed grass belonging to the Lamiaceae and has the pharmacological action of blue-heat damping and dehydration detoxification. It is used for the detoxification and fever of acute inflammation. By antibacterial and fungal inhibitory effect and remarkable diuretic effect is effective in the control of clear heat.

Geumeunhwa (金 꽃 花) is a bud of the honeysuckle, which belongs to the genus Phloxaceae. It has a pharmacological action of blue heat detoxification, which mainly contains inositol, luteolin, and tannin. It is a spectrum antimicrobial drug that has the effect of inhibiting influenza virus. It is a commercial drug for purulent skin disease. It must be used when there is redness and heat pain due to pus and swelling.

Mokcheonryum is a insect-infested fruit of the perennial plant and the related plant of the genus Tetaceae. Actinidine, Matataferlactone, and β-phenylethel alcohol are acted on the vagus nerve center to relieve inflammation. By sending out the uric acid, the effect of suppressing pain is excellent, it is used as an analgesic medicine, it treats numbness of the hands and feet, and works to improve blood circulation.

Myrrh is a rubber resin obtained from the myrrh tree or the same plant belonging to the olive tree family. The myrrh is converging by the pharmacological action of the livestock stems, which are mainly composed of fats and oils, essential oils and handing. There is a function of removing the phlegm and inhibiting many kinds of fungi, which can be used for joint pain.

Happiness is a terrestrial outpost of Jindukchal and cognate root plants belonging to the Asteraceae family. It is mainly used when the limbs are cold due to the customs, the knee is cold, and the pain is used, and it is suitable when there are symptoms such as quadriplegia and knee weakness.

The tree is a ripe fruit of the Chinese quince tree belonging to the Rosaceae family, and the roots of Tong Kyung-rak, such as Sugeun lak, whose main components are saponin, malic acid, tartaric acid, limonic acid, vitamin C, and tannin. It has a pharmacological action, which slows down cramps such as muscle strength of the lower limbs due to the customs, muscle movement paralysis and arthritis.

Baekji (白芷) is the root of perennial herbaceous guridae or its varieties belonging to the Araaceae family, and has pharmacological effects such as Geophae Haeum (소 風 解 表) and small bell pepper (으로), mainly composed of angellicin, angelicol, and angelicotoxin. It is used as an analgesic drug to relieve pain.

Hungae is a pharmacological action of the sperm of the hwalyeom belonging to the Lamiaceae, in the form of a flower, a giant wind haejugo (祛風 解 表) consisting mainly of essential oils containing l-mentol, dl-mentol and a small amount of d-limonene There is this. It has an antipyretic effect and makes the blood circulation of the skin vigorous and promotes the secretion of sweat glands to relieve spasms. It has the effect of promoting destruction and absorption of lesion tissue of the skin and eliminating itching.

Fruits are cut in half or in unripe fruits of tanza, which belongs to the mountain family, and are traces of essential oils consisting of limonene, essential oils, and flavons such as hesperidin and aurantiamarin. The pharmacological action of the back enhances the flow of the stomach, adjusts the flow rhythm and increases the tension.

Haedongpi (海桐 皮) is a bark of the oak tree belonging to the legumes, and has the pharmacological action of Geopungtongrak (하는 風 通 絡), which mainly contains alkaloid, etc., which is good for chronic joint rheumatism and especially for knee and leg pain caused by custom. good. It is used for skin fungus, and it is effective for pain and exercise recovery when it is used for joint pain, exercise disorder due to bruises, fractures, etc.

Pogong-young is an outpost of dandelion and the same plant belonging to the Asteraceae family, and has fungal detoxification pharmacological action mainly composed of crystalline high quality taraxacin, traraxarol and asparagin. It is used for acute inflammation by inhibiting the amino acid, and the amino group produced through the amino group transfer reaction is converted to uric acid and the transaminase level is high. It is applied to diseases and used as a general anti-inflammatory agent.

Euiyeongseon (威靈仙) is a root of the Eurasian colostrum plant belonging to the genus Minaria, and has a strong pharmacological effect of lichen pain, which is composed mainly of anemonin and anemol, etc. It is used for chronic joint rheumatism.

Killed (竹茹) is the middle layer removed from the outer shell of the paddle or royal family belonging to the family of rice family, has a pharmacological action, such as blue fever (화), hwadam (化痰) is mainly used as a supplement due to acute fever.

Hyunho-saeng (玄 胡 索) is a tuber of wild hyacinth or other similar plants belonging to the poppy family. It has 15 kinds of alkaloid-based pain and hypnotic pharmacological action to treat all the pain in our body. In particular, when applied to blunt pain caused by gaseous blood, such as joint pain, bruise injury pain, there is a certain analgesic effect.

Safflower is a plate made of the corolla of safflower belonging to the chrysanthemum as it is or by removing most of the yellow pigment and pressing it. ) It has pharmacological effects on the erythematous dysmenorrhea and amenorrhea. It is used for safflower uterus excitement and synovial liner.

Cheongung is a boiled boiled boiled as it is, or boiled in boiling water. It is an active blood locomotive, an active form of blood, which contains alkaloids, ferulic acid, essential oils, and some sort of 結晶 性 中性 物.祛風 止痛) pharmacological action. Essential oils act on the central nervous system to inhibit the activity of the cerebrum. Caffeine has the effect of suppressing the excitement. It inhibits various skin fungi and is used for muscle rheumatism and chronic joint rheumatism.

Root (牛膝) is the root of the iron knee belonging to the amaranth family, and it has pharmacological action such as saponin, a large amount of potassium salt, alkaloid-based beetle pain, and active blood circulation. . There is analgesic and diuretic effect, regardless of the cause of the license, customs, bruises sprains, etc. The lumbar pain is used for the lumbar pain, lumbar pain caused by the customs, pain, limb pain, limb pain.

Creation (蒼朮) is the root stem of fine leaf or Manchurian inserts belonging to the Asteraceae family, and it has pharmacological action such as essential oils containing atractylol, atractylon, etc. There is a tonic and sedative to tonic. It is an important drug to get rid of wind in the body. It can be used for both invasion and invasion, but creation is most effective for invasion. This action is used because when there is no strength in the whole body or you do not want to move.

Dermis is a mature skin of tangerine or related plant of the same family, which has a pharmacological effect such as hegiidin, myoinoisitol, d-limonene, and vitamin B1. Because it is strong, it is used for dyspepsia, stops nausea, eliminates phlegm in the body, and expands the airways, making breathing comfortable.

Baekchul (白 朮) is a rhizome that removes the cuticle of the inflorescence belonging to the chrysanthemum family, and it has pharmacological action such as 精油, which mainly contains atractylol, atractylol, and vitamin A. Its remarkable and persistent action inhibits the resorption of urine tubules and increases the excretion of kalium.

Yeon-kyo is the fruit of the forbidden forsythia, forsythia or chinese forsythia, belonging to the ash family, and has the antibacterial activity against the Staphylococcus aureus and S. aureus bacillus because it has a clear heat detoxification effect mainly composed of forsythol and oleanic acid. This is the strongest and suppresses the influenza virus. It has a diuretic effect and is purulent and is used when there are inflammatory symptoms such as fever.

Guernsey (乾 地 黃) is a dried root of the sulfur or other related plants of Hyunsam and is a blue-green blood blood (淸 熱 養血) mainly composed of rehmanin, xylitol, glucose, mannitol, iron, vitamin A, etc. , Yang Yin Saengjin (養陰 生 津) has the pharmacological action. Extracts promote blood coagulation and have a strong cardiac effect on the weakened heart. In particular, the extract acts on the heart muscle to aid fever and is used for recessive diseases with dehydration such as dry mouth and constipation.

Baekbokryeong (白 茯 笭) is a bacterium that almost eliminates the bark of the Baeryeong fungi belonging to the fungi family, and is composed of β-pachymose, pachymic acid, protein, fat, lecitin, histidine, and potassium salt. ), Dry nausea (健脾 和 中) has pharmacological effects such as anorexia, indigestion, diarrhea, etc. It is used for symptoms caused by nasal weakness. Used for Increases the release of Na, K, Cl, etc., and suppresses the resorption of urine tubules.

Licorice (甘草) is the root and gourds of European licorice, Manchurian licorice, and the same plants of legumes, as it is, or removed the bark, and contains liquiritigenin, glucose, mannitol, malic acid, and l-asparagine as main ingredients.氣), detoxification (解毒) and the like has a pharmacological action. Licorice is used to invigorate and revitalize the medicine as a medicine for detoxification. It has a corticoid effect such as storing moisture and Na in the body, increasing blood pressure and increasing K release.

Gilkyung is the root of the bellflower belonging to the campanula, or the bark is removed, and the pharmacology of the medicinal plants such as the greening machine (Golda) which is composed mainly of platyciside, inulin, phytosterol, etc. There is a function to promote the secretion of organs (氣管) to eliminate phlegm and to inhibit the ringworm in the test tube. It is used to discharge pus and is used for colds caused by fuan or wind heat such as cold, acute bronchitis and pneumonia.

Dokbyeong (獨 活) is the root of the poisonous tree belonging to the ogalpiaceae, has a small amount of essential oils, phytosterol and the pharmacological action, such as gongpungup (祛風濕), Tongkyeongrak (通 經絡). Animal experiments have anti-arthritis and hypnosis. Directly expands blood vessels to lower blood pressure, stimulates the respiratory center to enhance breathing, and is used for pain and numbness in muscles and lower body joints.

Ui-in (씨 仁) is a seed that removes the seed of Yulmu belonging to the rice family, and contains fatty oil, coixol, sterol, aminoacid, vitamin B1, etc. It is effective for muscle diseases such as reducing muscle spasms caused by customs. Diuretic sodam (利 尿 消痰), Geojeungjik (祛風 止痛), geonbi branch (健脾 止瀉) is used.

Baekjaja (白芥子) is a mature seed of mustard belonging to Cruciferaceae, and it has the pharmacological action of small sore throat, which contains sinalbin, fatty oil, sinalbinase, sinapine, etc. as a main ingredient to alleviate pain and promote inflammation removal. It is used for severe pain of muscles and joints. It is used to eliminate the symptoms of swollen parts of the human body due to inflammation or tumor by removing phlegm.

Chongbaek (蔥白) is a fresh root of leeks belonging to the family Liliaceae, sweat persimmon (통 解 表), tongyang (通 發汗) consisting mainly of sesame oil, malic acid, vitamin B, C, iron salts, etc.陽) and pharmacological action. It gives light stimulation to the bronchus, removes phlegm and has antibacterial effect. It is generally used as a supplement for sweating, such as headache, nasal congestion, and sweat-free symptoms. Difficulty in urination due to agitation of bladder and abdomen Used for bloating and abdominal pain.

In the present invention, the ratio of each herb when preparing the water extract is 10 to 18% by weight of gold, 7 to 14% by weight of silver and silver, 5 to 9% by weight of moths, 4 to 8% by weight, myrrh 3 to 7% by weight, neck and neck 3 To 7% by weight, 3 to 7% by weight of white paper, 3 to 7% by weight, 3 to 7% by weight of fruit fat, 3 to 6% by weight of thawed skin, 2 to 4% by weight of pogongyoung, 2 to 4% by weight of lieutenant stomach, 2-4 to kill % By weight, Corydalis 2-4%, Safflower 2-4%, Cheongung 2-4%, Dew 2-4%, Produce 1-3%, Dermal 1-3%, White 1-1% %, Yeongyo 0.5-1% by weight, 0.5-1% by weight Guernsey, 0.5-1% by weight, Baekbokyeong, 0.5-1% by weight licorice, 0.5-1% by weight, 0.5-1% by weight, Euiin 0.5-1% by weight It is preferable that it is 0.5-1 weight% of a white medium and 0.5-1 weight% of a total bag.

More preferably, about 14.09% by weight of gold, about 10.56% by weight of gold and silver, about 7.04% by weight of moth, about 5.63% by weight of myrrh, about 4.93% by weight, about 4.93% by weight of neck, about 4.93% by weight of white paper, about 4.93% of mold Weight%, Fruit about 4.93%, Thawing blood about 4.23%, Poongyoung about 3.17%, Maengyeong line about 3.17% by weight, Kill about 3.17% by weight, Corydalis about 3.17% by weight, Safflower about 2.82% by weight, Cheongung about 2.82% by weight About 2.82% by weight, creation about 2.12% by weight, about 2.12% by weight, dermis about 2.12% by weight, about 0.7% by weight of bridge bridge, about 0.7% by weight of dried liquor, about 0.7% by weight of Baekbokyeong, about 0.7% by weight of licorice About 0.7% by weight, about 0.7% by weight of poison, about 0.7% by weight of yiyiin, about 0.7% by weight of white dog, and about 0.7% by weight of total bag.

The 29 medicinal herb extracts of the present invention can be used for pharmaceuticals, foods, and the like.

At this time, the extract according to the present invention can be formulated on the basis of the formulation standard of the KFDA (KFDA) as a pharmaceutical formulation or a dietary supplement of dietary supplement.

The extract may be diluted in conventional manner, mixed with a pharmaceutically acceptable carrier, or enclosed in a carrier in a container form according to the administration method, dosage form, and therapeutic purpose.

When the carrier is used as a diluent, oral administration with at least one carrier selected from the group consisting of saline, buffer, dextrose, water, glycerol, Ringer's solution, lactose, sucrose, calcium silicate, methyl cellulose and ethanol For parenteral administration it may be prepared in formulations such as powders, granules, injections, syrups, solutions, tablets and the like. However, the carrier of the present invention is not limited to the above carrier. In this case, parenteral administration means administration of the active ingredient through rectal, intravenous, peritoneal, muscular, arterial, transdermal, nasal, inhalation, etc. in addition to orally.

The formulation may further include fillers, anti-coagulants, lubricants, wetting agents, flavors, emulsifiers, preservatives, and the like, and may be formulated to provide rapid, sustained or delayed release of the active ingredient after administration. And the dosage of the extract of the present invention can be adjusted according to the condition of the patient, the route of administration and the dosage form is not limited and those skilled in the art according to the symptoms can be obviously used within various ranges In general, in the present invention, it is determined that 2.9 to 4.3 ml per kg of body weight can be continuously or intermittently administered per day in an experimental effective amount.

On the basis of the effective amount of the extract, the present invention provides a functional food or food additives comprising the composition itself or a mixture of food-acceptable carriers, the functional food is processed meat products, fish meat products, tofu, jelly , Noodles, noodles such as ramen or noodles, seasoned foods such as soy sauce, miso, red pepper paste, mixed soy sauce, dairy products such as sauces, sweets, fermented milk and cheese, pickles such as kimchi and pickles, fruits, vegetables, soy milk, fermented beverages It may be in the form of a beverage, such as, the food additive may be used in addition to this type of food. This is obvious to those skilled in the art of the present invention will not be described the specific cooking method or production method thereof. In addition, the pharmaceutically acceptable carrier may also be used as the pharmaceutically acceptable carrier.

According to the present invention, the 29 medicinal herb extracts of the present invention contain a large amount of phenolic compounds, have high DPPH radical scavenging ability and ABTS radical scavenging ability, and exhibit excellent Xanthine oxidase inhibitory activity, thereby preventing, improving or preventing gout. It can be usefully used for treatment.

In addition, the extract of the present invention has fewer side effects because it is an edible medicinal raw material, especially when manufactured in the form of functional foods or food additives can be easily taken by anyone, and is expected to be more effective.

Hereinafter, the present invention will be described in more detail with reference to Examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

<Preparation of 29 Herbal Extracts>

The medicines of Table 1 used in this example were purchased at Gyeongdong Market, Seoul, from August to October 2012.

Common name Korean name Scientific name Rehmanniae Radix Guernsey Yellow (乾 地 黃) Rehmannia glutinosa Angelicae Dahuricae Radix White paper Angelicae dahurica Araliae Continentalis Radix Poisonous activity Aralia continentalis Kitagawa Atractylodes Rhizoma Creation Atractylodes lancea D.C Atractylodes rhizome white Baekchul (白 朮) Atractylodis ovata Scutellariae radix Golden Scutellaria baicalensis Gerorgi Platycodi Radix Gil-kyung Platycodon grandiflorum Nakai Bambusae caulis in taeniam Kill (竹茹) Phyllostachys bambusoides Clematidis radix Maternity Line Clematis terniflora var. Coix seed Ui-in Coix lacryma-jobi L. var Corydalis tuber Hyunho Color Corydalis ternate Nakai Taraxaci Herba Pogongyoung Taraxacum platycarpum Dahist. Citrus unshiu peel Dermis Citrus unshiu S. Marcov. Ciboule root Chongbaek Allium fistulosum L. Forsythia Fruit Fellowship Forsythia koreana Nakai Hoelen Paekbokyeong (白 茯 笭) Poria cocos wolf Honeysuckle flower Gold Silver Flower Lonicera japonica Thunberg Poncirus fruit Jisil Poncirus trifoliata Kalopanax Cortex Haedong Blood Kalopanax septemlobus Koidz. Licorice root Licorice Glycyrrhiza uralensis Fisch Mustard seed White Dog (白芥子) Sinapis alba Myrrh Myrrh Commiphora molmol Engler Chaenomelis Fructus Tree Chaenomeles sinensis Koehne Safflower Safflower Carthamus tinctorius L. Schizonepetae spica Brother-in-law Schizonepeta tenuifolia Var. Siegesbeckiae Herba Celebration Siegesbeckia glabrescens Makino Actinidiae fructus Mokcheon-ryo Actinidia polygama Maxim Cnidium Rhizome Cheonggung Cnidium officinale Makino Achyranthes root Wedge Achyranthes bidentata

Each medicine was put in burlap sacks according to the capacity of Table 2 (1,420g), and 13 liters of water (purified water) was added and extracted with a pressureless extractor (TOWER-KS220, Kyungseo, Korea) at 100-105 ° C for 3 hours 30 minutes. . The extract was transferred to an automatic standing pack packing machine (DONGJIN, Korea) through a filter and heated (sterilized) to 110 ° C., and then injected and packed into polyethylene packaging.

Serial number Medicinal name Capacity (g) ratio(%) One Gold 200 14.09 2 Gold silver 150 10.56 3 Neck charge 100 7.04 4 Myrrh 80 5.63 5 Celebration 70 4.93 6 Neck and neck 70 4.93 7 blank 70 4.93 9 Mold 70 4.93 10 Basement 70 4.93 8 Sea tangle 60 4.23 12 Poongyoung 45 3.17 16 A lieutenant ship 45 3.17 23 Kill 45 3.17 24 Corydalis 45 3.17 11 Safflower 40 2.82 15 Celestial 40 2.82 20 The 40 2.82 14 Creation 30 2.12 18 dermis 30 2.12 19 Boiling 30 2.12 21 Abalone 10 0.7 17 Guernsey 10 0.7 13 Baekbokryung 10 0.7 22 licorice 10 0.7 25 Gakyung 10 0.7 26 Solo 10 0.7 27 righteous person 10 0.7 28 A hundred 10 0.7 29 Total back 10 0.7 Sum 1420 100

<Effect of 29 Medicinal Herb Extracts>

The effects on the gout of the 29 medicinal herb extracts were verified through experiments.

Total phenol content

Phenolic compounds collectively refer to aromatic compounds having hydroxyl groups as main substances of the plant's secondary metabolites. The phenolic compound can inhibit the oxidation reaction because the phenol can provide hydrogen atoms to the radicals to stabilize the radicals and form resonance hybrids, which is relatively stable and difficult to react with oxygen to stabilize the free radicals. . In addition, the antioxidant activity of the phenolic component is known to be different depending on the structure, but in general, it is reported that the total phenolic content and the antioxidant activity of the plant component is correlated, so the extract phenol from the calibration curve prepared using gallic acid as a standard solution Material content was analyzed.

Total phenolic content was determined by modifying Gutfinger's method. That is, after diluting the extract 10 times, 1 ml was taken, 1 ml of 2% (w / v) Na ₂ CO ₃ solution was added thereto, and left for 3 minutes. Then, 0.2 ml of 50% Folin-Ciocalteu reagent was added and reacted for 30 minutes. It was left at room temperature. The mixture was centrifuged at 13,400 x g for 10 minutes, and then 1 ml of the supernatant was taken and absorbance was measured at 750 nm. The total phenol content was expressed as gallic acid equivalent (GAL) / mL compared to the standard curve prepared with gallic acid.

As a result, the total phenolic content of the water extract of the present invention showed a high value of 1.50 ± 0.05 mg GAL / ml.

DPPH radical scavenging ability

DPPH (1-dihpenyl-2-picrylhydrazyl), which is used for the measurement of electron donating ability, is a relatively stable radical that is chemically derived and has a deep purple color. It is reduced by receiving electrons or hydrogen, and the dark purple color is changed to a stable compound to give a yellowish color. At this time, the degree of discoloration is known to be a measure of antioxidant capacity.

DPPH radical scavenging ability was measured by adding 0.941 of 0.041 mM DPPH solution to 0.1 ml of the extract diluted 10-fold in accordance with Jeong's method and reacting at room temperature for 30 minutes to measure absorbance at 517 nm. DPPH radical scavenging ability of each sample was expressed as a percentage calculated by the following equation.

[formula]

DPPH radical scavenging activity (%) =

The DPPH radical scavenging ability of the extract of the present invention diluted 10-fold was 93.46 ± 0.13%, which was higher than that of 100 μg / ml L-ascorbic acid (89.38 ± 1.52%).

ABTS radical scavenging ability

ABTS assay is different from DPPH assay that eliminates free radicals by eliminating cationic radicals, and it is a method to measure antioxidant power by decolorizing blue-green color.

ABTS radical scavenging ability was measured according to the method of Pellegrini et al. To 100 μl of the extract diluted 10-fold, 100 μl of 0.1 M phosphate buffer (pH 5.0) and 20 μl of 10 mM hydrogen peroxide were added, and the mixture was preliminarily reacted at 37 ° C. for 5 minutes. Add 30 μl of 1.25 mM ABTS and peroxidase (1U / mL) to the reaction, and react for 10 minutes at 37 ° C. Then, at 405 nm, use Multiplate Reader (Sunrise RC / TS / TS Color-TC / TW / BC / 6 Filter). , Tecan Austria GmbH, Austria) was used to measure the absorbance.

[formula]

ABTS radical scavenging activity (%) =

The ABTS radical scavenging ability of the extract of the present invention diluted 10-fold was 89.82 ± 0.16%. The control group, L-ascorbic acid, showed 95.66% of activity at 100㎍ / ㎖.

Xanthine oxidase  Inhibitory activity

Xanthine oxidase is an enzyme that is involved in purine metabolism in vivo and forms uric acid from xanthine or hypoxanthine, and when uric acid is excessively produced and increased in plasma, it is deposited on joints, tissues and kidneys and causes inflammation, resulting in pain and kidneys. It is known to cause disease.

Xanthine oxidase inhibitory activity was performed according to the method of Sirpe and corte. 0.1 ml of 5-fold extract and 0.6 ml of 0.1 M potassium phosphate buffer (pH 7.5) were added to 0.2 ml of substrate solution in which xanthine 2 mM was dissolved. After 0.1 ml of xanthine oxidase (0.2 U / ml) was added thereto and reacted at 37 ° C. for 5 minutes, 1 ml of 1N HCl was added to stop the reaction, and then the uric acid generated in the reaction solution was measured at an absorbance of 292 nm. The xanthine oxidase inhibitory activity of the extract was expressed as a percentage (%) of the absorbance of the sample addition and no addition. The xanthine oxidase inhibitory activity was measured by the same method using ascorbic acid instead of the extract as a control.

The xanthine oxidase inhibitory activity of the extract of the present invention diluted five-fold was 19.28 ± 1.20%. This is higher activity compared to 100 μg / ml L-ascorbic acid (11.45 ± 1.21%).

As a result, the extract of the present invention has xanthine oxidase inhibitory activity, and thus is expected to be effective for the treatment and improvement of diseases induced by gout and xanthine oxidase.

Statistical processing

All experiments were repeated three times, and the average value was processed according to the method of General Linear Model using SPSS software (Ver. 12). Percentage and mean ± standard error were calculated according to each item, and the difference of all treatment values was analyzed by comparing with 95% confidence level (p <0.05).

Correlation between experiments

The correlation between the total phenolic content, DPPH radical scavenging activity, ABTS radical scavenging activity and xanthine oxidase inhibitory activity was analyzed. , DPPH radical scavenging activity was relatively low correlated with xanthine oxidase inhibitory activity. Although there is no correlation between the inhibitory activity and the radical scavenging activity of the enzyme, it is reported that tannin and tannin-related substances such as ellagic acid, which strongly inhibit Xanthine oxidase, also have radical scavenging ability. It is presumed that this would be inhibited by the effects of tannins and their related substances rather than the effects.

<Effectiveness of 29 medicinal herb extracts for gout patients>

The patient was diagnosed with gout and provided with 29 extracts of extracts in 100ml increments. One adult (weight average 70kg, most patients with gout) had 2 per day. Take 30 days (morning, afternoon). As a rule, when there is pain, 1 to 2 packs are taken as necessary to investigate the relief of pain caused by gout.

Twelve patients with gout were measured and pain relief was measured on a five-point scale (5, pain was alleviated; 4, pain was alleviated; 3, no difference; 2, pain was worsening; 1, Pain was very worse).

patient Pain relief One 5 2 5 3 5 4 5 5 5 6 5 7 5 8 3 9 4 10 3 11 4 12 5 Sum 54 Average 4.5

As a result of the evaluation, as shown in Table 3, most patients felt that the pain was relieved by taking the extract of the present invention.

Claims (9)

Golden, silver and gold, myrrh, myrrh, bliss, tree and white paper, hyungge, jisil, haedongpi, pogongyoung, lieutenant ship, kill, hyunho colour, safflower, cheongung, wolden, creation, dermis, baekryeok, kyoji, gunjihwang, baekbokyeong, licorice , Venom, uiyiin, gyeokgwa and ginseng extracts containing the water extract as an active ingredient pharmaceutical composition for preventing or treating gout. The method of claim 1,
The water extract is
10 to 18% by weight of gold, 7 to 14% by weight of silver and silver, 5 to 9% by weight, molar about 4 to 8%, 3 to 7% by weight, 3 to 7% by weight of neck, 3 to 7% by weight of white paper, 3 to 7% by weight, 3 to 7% by weight of fruit, 3 to 6% by thawing blood, 2 to 4% by weight, 2 to 4% by weight of gastric glands, 2 to 4% by weight, 2 to 4% by weight of magenta color, safflower 2 To 4% by weight, 2 to 4% by weight, 2 to 4% by weight, 1 to 3% by weight, 1 to 3% by weight, 1 to 3% by weight of dermis, 1 to 3% by weight, 0.5 to 1% by weight, 0.5 to 1% of dried fish 1% by weight, Baekbokyeong 0.5-1% by weight, licorice 0.5-1% by weight, 0.5-1% by weight length, 0.5-1% by weight, Euiin 0.5-1% by weight, 0.5-1% by weight, and 0.5-1 by weight A pharmaceutical composition for preventing or treating gout, characterized in that it is extracted from the mixture by weight. 3. The method of claim 2,
The water extract is
A pharmaceutical composition for preventing or treating gout, wherein the extract is obtained by adding 5 to 20 times the weight of water to the mixture and extracting it at 90 to 110 ° C. for 2 to 5 hours. Golden, silver and gold, myrrh, myrrh, bliss, tree and white paper, hyungge, jisil, haedongpi, pogongyoung, lieutenant ship, kill, hyunho colour, safflower, cheongung, wolden, creation, dermis, baekryeok, kyoji, gunjihwang, baekbokyeong, licorice , Ginseng, uiyiin, ginseng and ginseng extracts containing ginseng extract as an active ingredient for preventing or improving functional foods. 5. The method of claim 4,
The water extract is
10 to 18% by weight of gold, 7 to 14% by weight of silver and silver, 5 to 9% by weight, molar about 4 to 8%, 3 to 7% by weight, 3 to 7% by weight of neck, 3 to 7% by weight of white paper, 3 to 7% by weight, 3 to 7% by weight of fruit, 3 to 6% by thawing blood, 2 to 4% by weight, 2 to 4% by weight of gastric glands, 2 to 4% by weight, 2 to 4% by weight of magenta color, safflower 2 To 4% by weight, 2 to 4% by weight, 2 to 4% by weight, 1 to 3% by weight, 1 to 3% by weight, 1 to 3% by weight of dermis, 1 to 3% by weight, 0.5 to 1% by weight, 0.5 to 1% of dried fish 1% by weight, Baekbokyeong 0.5-1% by weight, licorice 0.5-1% by weight, 0.5-1% by weight length, 0.5-1% by weight, Euiin 0.5-1% by weight, 0.5-1% by weight, and 0.5-1 by weight Functional food for preventing or improving gout, characterized in that water extracted from the mixture by weight. 6. The method of claim 5,
The water extract is
Functional food for preventing or improving gout, characterized in that the extract obtained by adding 5 to 20 times the weight of water to the mixture and extracted for 2 to 5 hours at 90 to 110 ℃. Golden, silver and gold, myrrh, myrrh, bliss, tree and white paper, hyungge, jisil, haedongpi, pogongyoung, lieutenant ship, kill, hyunho colour, safflower, cheongung, wolden, creation, dermis, baekryeok, kyoji, gunjihwang, baekbokyeong, licorice , Ginseng, uiyiin, ginseng and ginseng extracts containing ginseng extract as an active ingredient as an active ingredient. 8. The method of claim 7,
The water extract is
10 to 18% by weight of gold, 7 to 14% by weight of silver and silver, 5 to 9% by weight, molar about 4 to 8%, 3 to 7% by weight, 3 to 7% by weight of neck, 3 to 7% by weight of white paper, 3 to 7% by weight, 3 to 7% by weight of fruit, 3 to 6% by thawing blood, 2 to 4% by weight, 2 to 4% by weight of gastric glands, 2 to 4% by weight, 2 to 4% by weight of magenta color, safflower 2 To 4% by weight, 2 to 4% by weight, 2 to 4% by weight, 1 to 3% by weight, 1 to 3% by weight, 1 to 3% by weight of dermis, 1 to 3% by weight, 0.5 to 1% by weight, 0.5 to 1% of dried fish 1% by weight, Baekbokyeong 0.5-1% by weight, licorice 0.5-1% by weight, 0.5-1% by weight length, 0.5-1% by weight, Euiin 0.5-1% by weight, 0.5-1% by weight, and 0.5-1 by weight A food additive for preventing or improving gout, characterized in that it is extracted from the mixture by weight. The method of claim 8,
The water extract is
The food additive for preventing or improving gout, characterized in that the extract obtained by adding 5 to 20 times the weight of water to the mixture and extracted for 2 to 5 hours at 90 to 110 ℃.