KR101273426B1 - Manufacturing method of polyphenols fortified feed additives using grape by-product and propolis extract - Google Patents

Abstract

PURPOSE: A manufacturing method of farming additives for domestic cattle or fish is provided to strengthen the content of resveratrol per feed additive unit weight and improves the functionality and prevention of diseases with even combination of propolis. CONSTITUTION: Grape skin, grape seed, and a bunch of grapes are separated due to the parts and respectively fermented at 3-5°C for 2 to 4 months. Fermented pericarp, grape seed, and a bunch of branches are separated and dried until 10% of the water content at 45-60°C. 15-25 parts by weight of dried grape seed, 40-60 parts by weight of dried bunch of branches, and 15-25 parts by weight of tartaric acid are mixed and coarse-grinding. 30-100 parts by weight of refined propolis extract powder are evenly mixed and vacuum dried until 5% of water contents about 100 parts by coarse weight. Vacuum dried materials are pulverized to a particle size of 100-120 mesh. Refined propolis extract powders are pulverized under freeze-drying.

Description

Manufacturing method of Polyphenols fortified Feed additives using Grape by-product and Propolis Extract}

The present invention relates to a method for preparing a feed additive for livestock or fish farming, and more particularly, to a method for preparing a feed additive for livestock or fish farming, reinforced with polyphenols using grape byproducts and propolis extracts.

With the development of the food processing industry using agricultural products, a large amount of agricultural waste is emitted in the production process of commercializing agricultural products as processed foods, and grapes are also processed and processed into grapes in the process of producing various processed foods such as wine, canned food and beverages. Large quantities of grape by-products such as seeds, seeds and clusters are being released.

Grape contains high levels of polyphenol-based resveratrol, an antioxidant found in plants, and resveratrol has a strong antioxidant activity with antibacterial, anticancer, antiviral, antiallergic, and anti-inflammatory effects. As it is a substance with a large amount of grape by-products such as skin, seeds, and pine branches, if you dispose of grape by-products, you will not only lose useful components contained in agricultural products, but also the problem of environmental pollution. Research to increase value is actively underway.

As a technique using resveratrol contained in grape by-products, for example, Korean Laid-Open Patent Publication No. 10-2009-0092405 discloses extracting grape stems with ethanol, and then extracting resveratrol using grape stems by centrifugation and reduced pressure evaporation after sonication. The Korean Patent Publication No. 10-1068890 discloses a method of extracting polyphenols from grape branches by organic solvents, then evaporating and removing the organic solvents, and extracting and condensing polyphenols from grape branches to obtain polyphenols. However, the prior art is a technology for extracting resveratrol and the like by using an organic solvent as a grape by-product as a technique for separating only the polyphenol, which is an active ingredient contained in grape by-products, the extraction residues still have to be discarded as a whole grape by-products Preparation of Feed Additives Using Efficiently Mothada is not suitable to apply the law.

In addition, Korean Patent Publication No. 10-0872617 discloses a method of manufacturing a feed additive by drying and grinding fermented products fermented with yeast using juice and grains obtained by pressing grape by-products. 10-1162955 discloses a method of preparing a feed additive for livestock using grape byproducts which are fermented to mature grape by-products at 10-20% moisture content with Acetobacter Pasteruianus strains, and then irradiated with gamma rays to the dried and ground dry powder. However, the prior art is manufacturing a feed additive using grape by-products, but simply using the grape by-products do not efficiently use the resveratrol contained in the grape by-products there is a limit to the production of high-quality feed additives .

The present invention has completed the present invention by producing a higher-quality feed additive by efficiently using grape by-products having a difference in the content of resveratrol for each part such as skin, seed, and pine branch.

An object of the present invention is to provide a method for preparing a feed additive for livestock or fish farming using polyphenol-enhanced polyphenols using grape by-products and propolis extracts, such as grape skin (peel), grape seed and grape vine branch More specifically, grape by-products are divided into parts based on the fact that the content of polyphenol-based resveratrol is different in each part of the skin (grape peel), grape seeds, and grape cluster branches. It is an object of the present invention to provide a method for producing a feed additive for livestock or fish farming of polyphenol-enhanced using grape by-products and propolis extracts.

Solution to a method for preparing a feed additive for livestock or fish farming fortified with polyphenols using grape by-products and propolis extract for achieving the object of the present invention, grape skins (peel), grape seeds and grape clusters Branches were isolated for each long time, and then independently ripened for a long time and dried for a long time, and then, the dried bark, grape seed, and pine branches were coarsely pulverized with tartaric acid, and then purified by crude powder. The powders of the poly-solids are mixed uniformly, followed by pulverization after vacuum drying.

One of the characteristics of the method of preparing a feed additive for livestock or fish farming fortified with polyphenols using the grape by-product and propolis extract according to the present invention is grape by-products (skin) ), Grape seed and grape cluster branch (Congji branch) using the point showing the difference in the content of resveratrol by region, each of the skin peel, grape seed and cluster branch by aging and drying independently, then dried the dried rind, dried By properly blending and pulverizing grape seeds and dried clustered branches, the content of resveratrol per unit weight of the feed additive can be enhanced as compared to the feed additive using known grape byproducts.

Grape varieties
ingredient Resveratrol content (㎍ / g) Dry matter Non-Dry (Bio)
Geobong
(Gerbong) Grape peel 5 <1 Grape seed 6 4 Pine branch 170 39
Campbell
(Campbell) Grape peel 5 <1 Grape seed 5 3 Pine branch 411 121
Sheridan
Sheridan) Grape peel 8 One Grape seed 4 2 Pine branch 440 110

More specific means for achieving the object of the present invention includes a) a step of aging the grape by-products peeled off by the skin, grape seed and pine branch for 2 to 4 months at 3 ~ 5 ℃, respectively, b) aged The first drying step of drying the skin, grape seeds and pine branches in a separated state to a moisture content of 10% at 45 ~ 60 ℃, c) 15 to 25 parts by weight of dried grape seeds with respect to 100 parts by weight of dried skin, 40 to 60 parts by weight of the prepared pine branch and 15 to 25 parts by weight of tartaric acid, and coarsely pulverized step, d) 10 to 100 parts by weight of purified propolis powder with respect to 100 parts by weight of coarsely ground And a second drying step of vacuum drying at a water content of 5% and e) a fine grinding step of pulverizing the vacuum dried material.

The a) ripening step according to the present invention by the enzymatic action and natural fermentation of nutrients contained in grape by-products while storing grape by-products such as fruit skin, grape seeds and pine branches collected during the grape harvest process or grape processing process for a long time without alteration As a step of decomposing, it is possible to increase useful lactic acid bacteria and store for 2 to 4 months through the process of ripening at low temperature of 3 ~ 5 ℃, and a) in the ripening step, the collected grape by-products such as skin, grape seed and pine branch Each part is separated and matured, and grape seeds are preferably aged by grinding.

B) The primary drying step is to separate fermentation of the mature grape byproduct by separating the respective parts, thereby preventing the deterioration of the grape byproduct in the feed manufacturing process and smoothly performing the subsequent coarse grinding step. By drying at 45 ~ 60 ℃ water content up to 10%, b) the primary drying step also consists of performing the drying in each separated state for each part of the grape by-products.

And c) coarsely crushing step according to the present invention is 15 to 25 parts by weight of dried grape seeds 15 to 25 parts by weight, 40 ~ 60 parts by weight and tartaric acid 15 to 25 parts by weight of the dried grape by-products separated by 100 And pulverizing using a pulverizing means equipped with a stirring means such as a screw for uniform mixing and pulverization of the compound.

The blending ratio of the grape by-products is a blending ratio that can enhance the content of resveratrol per unit weight of the feed additive based on the content difference of the resveratrol shown in [Table 1], but the a) ripening step and b) primary When ripening and drying in a mixed state without separating the skin by-products, grape seed and pine branch, which are grape by-products in the drying step, it is not possible to mix the skin, grape seed and pine branch with the blending ratio according to the present invention in the crude grinding step. In the present invention, grape by-products such as peel, grape seed and pine branch should be separated and matured and dried.

In addition, when the blending ratio of dried grape seeds is 15 to 25 parts by weight, the content of useful components such as protein, dietary fiber, vegetable sterols as well as unsaturated fatty acids such as oleic acid and linoleic acid are reduced. Feed additives cannot be obtained, and as shown in [Table 1], the content of resveratrol is high in pine branch, but excessive composition of pine branch as feed additive may affect the palatability due to the fiber of eggplant. It is preferable to mix 40 ~ 60 parts by weight of pine branch with respect to 100 parts by weight of the skin, and the addition of tartaric acid is generally added to improve the digestibility of protein by reducing the pH in the stomach of livestock, such as the addition of organic acid. .

The d) secondary drying step according to the present invention is a step of vacuum drying up to 5% moisture content in order to efficiently perform the subsequent milling step, the propolis 30 per 100 parts by weight of the crude pulverized grape by-product before vacuum drying To 100 parts by weight, and vacuum drying.

The propolis is a known substance that has the effects of antibacterial, anti-inflammatory, anti-oxidant, anti-cancer, anti-viral, increased immunity, analgesic and anesthesia, and contains various various components including organic matter and minerals (inorganic salts). In particular, minerals, vitamins, amino acids, fats, organic acids, flavonoids, etc. play an important role in cell metabolism and immune system enhancement.

In the present invention, the purified propolis is composed of a powdered state for uniform blending with the coarsely pulverized grape by-product, the liquid extract of the purified propolis is very high viscosity and mixed uniformly with the coarsely pulverized grape by-product It is very difficult to do, and the concentrated solid propolis extract is hard to be pulverized and powdered as a viscous solid material in the present invention, so in the present invention, the solid propolis extract is pulverized in a freeze-dried state to obtain a powdery state. Propolis is blended uniformly by blending with coarsely milled grape by-products.

The freeze-dried powder of the propolis is rapidly cooled to about -10 ℃ of the purified solid ingot containing the propolis 95 ~ 98% coarsely pulverized to 20 ~ 30g weight and then 100 mesh using a high speed cold mill again It consists of pulverizing and powdering below.

Finally, the e) grinding step according to the present invention is a step of obtaining a feed additive by grinding the propolis-containing grape by-product to a particle size of 100 ~ 120 mesh, in the grinding step by the heat generated by the frictional heat during the grinding step It is preferable to use a grinder equipped with a cooling means because the phenomenon of burning water has a bad effect such as deterioration of the feed additive.

The pulverized feed additives described above are used in combination with commercial feed for livestock or fish farming, which are formulated with fish meal, grain flour, corn, bran, and other nutrients. 0.1 to 1 part by weight is used based on 100 parts by weight of basic feed for livestock, and 0.5 to 5 parts by weight is used for commercial fish based on 100 parts by weight of commercial feed for fish farming. For example, the world's first star (manufacturer's world's first feed).

The present invention separates grape by-products showing the difference in resveratrol content by parts such as grape skin (peel), grape seed and grape berry branch (song branch) by aging and drying the grape by-products such as peel, grape seed and cluster branches It is formulated at an appropriate ratio to enhance the content of resveratrol per unit weight of feed additives, and the uniform addition of propolis has the characteristics of obtaining feed additives for livestock and fish farming, which further improves functionality such as palatability and disease prevention.

EXAMPLES Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to the following Examples.

<Examples>

1) Ripening and primary drying stage

Hot air dryer (Samheung Energy Co., Ltd.) that controls the temperature, time, etc. by putting each of the skins, grape seeds, and pine branches, ripened for 2 months in a aging warehouse kept at 4 ° C in each storage container, in several trays. The oven of the grape by-product was mounted on a shelf of a manufactured dry oven, and dried at 50 ° C. for 7 hours with hot air to obtain dried products of grape skin, grape seed, and pine branch with a water content of 10%.

2) Crude grinding step

The dried product of each of the grape skin, grape seed and pine branch is blended at a ratio of 20 parts by weight of dried grape seed, 50 parts by weight of dried pine branch and 20 parts by weight of tartaric acid, and 100 parts by weight of dried pine skin, and control of mixing speed and the like. Coarse pulverized product was obtained by pulverizing using a screw crusher equipped with a function (manufactured by Samheung Energy, Inc.).

3) Vacuum drying step

A vacuum dryer (Samheung Co., Ltd.) having a control function such as vacuum, temperature, time, and the like, uniformly blended with 40 parts by weight of propolis extract powder, which was previously lyophilized and powdered, to 100 coarse ground products of the grape by-product obtained above. Energy drying, vacuum dry oven) was dried for 6 hours at 40 ℃ conditions to obtain a vacuum dried product of 5% moisture content.

4) Grinding stage

The feed additives pulverized to a particle size of 120 mesh were obtained by using a pulverizer (manufactured by Samheung Energy Co., Ltd.) equipped with a cooler for cooling the heat generated during the pulverization process and controlling the vacuum dried product obtained above. .

&Lt; Test Example 1 >

In the above example, 100 parts by weight of commercial livestock basic feed (Cheonha First Start No. 1, manufacturer Cheonha First feed) mainly composed of corn meal and soybean meal for the test on the productivity according to the biogrowth and feed intake of livestock Test feed formulated with 0.4 parts by weight (feed 1) and 0.8 parts by weight (feed 2) of the prepared feed additives. Three-way hybrid weight 6.68 ± 1.03kg Is shown in Table 2 below.

Item Control group (basic feed) Feed 1 Feed 2
0 ~ 2 weeks Biogrowth (g) / day 352 382 389 Feed intake (g) / day 401 400 401 Feed efficiency 0.878 0.955 0.970
2-5 weeks Biogrowth (g) / day 530 560 564 Feed intake (g) / day 732 757 704 Feed efficiency 0.724 0.740 0.801
Average Biogrowth (g) / day 459 489 495 Feed intake (g) / day 600 614 583 Feed efficiency 0.765 0.796 0.849

As shown in the above [Table 2], in the early days of weaning pigs, the daily growth rate (bio growth rate (g) / day) of the present invention was significantly higher than that of the control group, and the nutrient digestibility (feed efficiency), palatability It can be seen that the feed of the present invention is shown well.

&Lt; Test Example 2 &

Commercial fish farming base consisting of 46% high protein and 15% crude fat for testing against hemorrhagic septicemia (VHS) and streptococcus iniae-related diseases, the most problematic in flounder culture. Start, the manufacturer's Cheonha First Feed) A test feed containing 1.5 parts by weight (feed a) and 3 parts by weight (feed b) of the feed additive prepared in the above Example, based on 100 parts by weight of the plant, at Aewol-eup, Jeju Island, Korea. The purchased flounder (8.94 ± 0.02 g) 1,080 animals were placed in 24 tanks of 45 animals each, and feed was fed twice a day for 26 days, and the results for survival are shown in [Table 3] below. Indicated.

Survival rate (%) Control group (basic feed) Feed a Feed b VHS 48.9 ± 13.9 71.1 ± 25.2 93.3 ± 6.7 Streptococcus
iniae
24.4 ± 21.4
73.3 ± 29.1
80.0 ± 29.1

As shown in Table 3 above, the feed of the present invention was compared with the control group of viral hemorrhagic sepsis (VHS) virus and Streptococcus . Survival rate for fish attack of iniae is very high, so it can be predicted that the combination of the feed according to the present invention may have antibiotic replacement effect when used as a feed additive for fish farming .

Claims (6)

a) ripening step of separating the grape by-products of the skin (grape), grape seed and grape cluster branches for each part 2 to 4 months at 3 ~ 5 ℃,
b) a first drying step of drying the aged rind, grape seed and clustered branches in a separated state to a water content of 10% at 45 ~ 60 ℃,
c) a coarsely pulverizing step of blending 15 to 25 parts by weight of dried grape seeds, 40 to 60 parts by weight of dried pine branch and 15 to 25 parts by weight of tartaric acid, and coarsely pulverizing with respect to 100 parts by weight of the dried skin;
d) a secondary drying step of vacuum-drying to 30% water content by uniformly mixing 30 to 100 parts by weight of the purified propolis extract powder with respect to 100 parts by weight of coarse powder;
e) a method of producing a feed additive for livestock or fish farming fortified with polyphenols using grape by-products, characterized in that it comprises a fine grinding step of pulverizing the vacuum dried product. The method of claim 1, wherein the milling step is a method for preparing a feed additive for livestock or fish farming using polyvinyl byproducts, which is ground to a particle size of 100 to 120 mesh. The method of claim 2, wherein the purified propolis extract powder is powdered under lyophilization. Animal or fish feed additives fortified with polyphenols using grape by-products, characterized in that produced by the method of any one of claims 1 to 3. The method of claim 4, wherein the feed additive Livestock feed additives characterized in that the compounding 0.1 to 1 parts by weight based on 100 parts by weight of commercial feed for livestock. The feed additive for fish farming according to claim 4, wherein the feed additive is blended at 0.5 to 5 parts by weight based on 100 parts by weight of commercial basic fish feed.