KR101097560B1 - 핵산 검출 방법 - Google Patents

핵산 검출 방법 Download PDF

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KR101097560B1
KR101097560B1 KR1020107016726A KR20107016726A KR101097560B1 KR 101097560 B1 KR101097560 B1 KR 101097560B1 KR 1020107016726 A KR1020107016726 A KR 1020107016726A KR 20107016726 A KR20107016726 A KR 20107016726A KR 101097560 B1 KR101097560 B1 KR 101097560B1
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nucleic acid
pcr
amplification
sars
dna
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KR20100090730A (ko
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쳉 호이 유
록 팅 라우
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하이 캉 라이프 코포레이션 리미티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
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  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pulmonology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
KR1020107016726A 2003-05-02 2004-04-30 핵산 검출 방법 Expired - Fee Related KR101097560B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0310181A GB2401175A (en) 2003-05-02 2003-05-02 Detection of SARS virus by PCR
GB0310181.3 2003-05-02

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
KR1020057020758A Division KR101158934B1 (ko) 2003-05-02 2004-04-30 핵산 검출 방법

Publications (2)

Publication Number Publication Date
KR20100090730A KR20100090730A (ko) 2010-08-16
KR101097560B1 true KR101097560B1 (ko) 2011-12-22

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ID=33155801

Family Applications (4)

Application Number Title Priority Date Filing Date
KR1020107016726A Expired - Fee Related KR101097560B1 (ko) 2003-05-02 2004-04-30 핵산 검출 방법
KR1020057020758A Expired - Fee Related KR101158934B1 (ko) 2003-05-02 2004-04-30 핵산 검출 방법
KR1020127020392A Ceased KR20120094155A (ko) 2003-05-02 2004-04-30 핵산 검출 방법
KR1020117004874A Abandoned KR20110027854A (ko) 2003-05-02 2004-04-30 핵산 검출 방법

Family Applications After (3)

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KR1020057020758A Expired - Fee Related KR101158934B1 (ko) 2003-05-02 2004-04-30 핵산 검출 방법
KR1020127020392A Ceased KR20120094155A (ko) 2003-05-02 2004-04-30 핵산 검출 방법
KR1020117004874A Abandoned KR20110027854A (ko) 2003-05-02 2004-04-30 핵산 검출 방법

Country Status (8)

Country Link
US (1) US20080090224A1 (https=)
EP (2) EP2597162A1 (https=)
JP (2) JP4950656B2 (https=)
KR (4) KR101097560B1 (https=)
CN (1) CN1798842A (https=)
GB (1) GB2401175A (https=)
TW (3) TWI362419B (https=)
WO (1) WO2004097021A1 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101603096A (zh) * 2009-05-04 2009-12-16 北京海康基因芯片开发有限公司 一种检测传染病病原体的方法及试剂盒
CN102424863B (zh) * 2012-01-06 2013-06-12 海康生物科技（北京）有限公司 一种增强型荧光定量pcr方法
US11130994B2 (en) * 2019-12-13 2021-09-28 Autonomous Medical Devices Inc. Automated, cloud-based, point-of-care (POC) pathogen and antibody array detection system and method
US12416053B2 (en) * 2020-02-04 2025-09-16 Indian Institute Of Technology Delhi Primer sets, biomarkers, kit and applications thereof
US11649511B2 (en) * 2020-02-06 2023-05-16 Daan Gene Co., Ltd. Multiplex PCR method for the detection of SARS-CoV-2
IT202000006754A1 (it) 2020-03-31 2021-10-01 Diasorin S P A Saggi per la rivelazione di SARS-CoV-2
US11149320B1 (en) 2020-03-31 2021-10-19 Diasorin S.P.A. Assays for the detection of SARS-CoV-2
CN111455102A (zh) * 2020-04-09 2020-07-28 上海符贝基因科技有限公司 用于新冠病毒SARS-CoV-2基因组靶向测序的捕获探针制备方法
AU2021202504A1 (en) * 2020-04-29 2021-11-18 Teleflex Medical Incorporated System for identifying severe acute respiratory syndrome corona virus 2 (sars-cov-2) ribonucleic acid (rna)
CN111635960B (zh) * 2020-05-06 2023-06-27 温州医科大学附属眼视光医院 用于稳态速效检测新型冠状病毒的保护序列、引物、探针、组合物、试剂盒及应用和方法
WO2021247398A1 (en) * 2020-05-30 2021-12-09 Pangolin Llc Systems and methods to detect pathogens
JPWO2022030629A1 (https=) * 2020-08-07 2022-02-10
US11361847B1 (en) 2021-02-06 2022-06-14 Timothy A. Hodge System and method for rapidly reporting testing results
KR102625074B1 (ko) * 2021-04-20 2024-01-16 재단법인 아산사회복지재단 마이크로입자 기반 핵산 검출용 키트 및 핵산 증폭산물 검출 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1493602A (en) * 2000-10-05 2002-04-15 Hong Kong Dna Chips Ltd A kit for detecting non-pathogenic or pathogenic influenza a subtype h5 virus
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
AU2002346660A1 (en) * 2001-11-30 2003-06-17 University Of Rochester Multiplex real-time quantitative pcr
WO2004085455A1 (en) * 2003-03-24 2004-10-07 The University Of Hong Kong A diagnostic assay for the human virus causing severe acute respiratory syndrome (sars)
CN102021147B (zh) * 2003-03-24 2016-08-03 港大科桥有限公司 引起严重急性呼吸道综合征(sars)的新型人病毒及其应用
ES2529736T3 (es) * 2003-04-10 2015-02-25 Novartis Vaccines And Diagnostics, Inc. Composición inmunogénica que comprende una proteína espicular del coronavirus del SARS
EP1616038A2 (en) * 2003-04-21 2006-01-18 Genome Institute of Singapore Reagents and methods for detecting severe acute respiratory syndrome coronavirus
CN1442488A (zh) * 2003-05-16 2003-09-17 上海晶泰生物技术有限公司 传染性非典型肺炎（sars）病毒复合荧光定量pcr检测

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Clin., Chem., Vol.49, No.6, pp.953-955(2003.04.18.)

Also Published As

Publication number Publication date
KR20110027854A (ko) 2011-03-16
KR20050118738A (ko) 2005-12-19
CN1798842A (zh) 2006-07-05
WO2004097021A1 (en) 2004-11-11
JP4950656B2 (ja) 2012-06-13
TWI362419B (en) 2012-04-21
EP1620556A4 (en) 2010-09-15
TW201139687A (en) 2011-11-16
JP2012080884A (ja) 2012-04-26
TW201245452A (en) 2012-11-16
EP1620556A1 (en) 2006-02-01
TWI377255B (en) 2012-11-21
EP2597162A1 (en) 2013-05-29
US20080090224A1 (en) 2008-04-17
KR20100090730A (ko) 2010-08-16
TW200502400A (en) 2005-01-16
GB2401175A (en) 2004-11-03
KR20120094155A (ko) 2012-08-23
JP2006524996A (ja) 2006-11-09
KR101158934B1 (ko) 2012-07-04

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