KR100954641B1 - 아미노펩티다아제 - Google Patents

아미노펩티다아제 Download PDF

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Publication number
KR100954641B1
KR100954641B1 KR1020047003708A KR20047003708A KR100954641B1 KR 100954641 B1 KR100954641 B1 KR 100954641B1 KR 1020047003708 A KR1020047003708 A KR 1020047003708A KR 20047003708 A KR20047003708 A KR 20047003708A KR 100954641 B1 KR100954641 B1 KR 100954641B1
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South Korea
Prior art keywords
polypeptide
cell
aminopeptidase
sequence
human
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Expired - Lifetime
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KR1020047003708A
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English (en)
Korean (ko)
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KR20040041611A (ko
Inventor
존씨. 졸리
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제넨테크, 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
KR1020047003708A 2001-09-13 2002-09-12 아미노펩티다아제 Expired - Lifetime KR100954641B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US32235001P 2001-09-13 2001-09-13
US60/322,350 2001-09-13
PCT/US2002/029015 WO2003023030A2 (en) 2001-09-13 2002-09-12 Aminopeptidase

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020087030860A Division KR100956907B1 (ko) 2001-09-13 2002-09-12 아미노펩티다아제

Publications (2)

Publication Number Publication Date
KR20040041611A KR20040041611A (ko) 2004-05-17
KR100954641B1 true KR100954641B1 (ko) 2010-04-27

Family

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Family Applications (2)

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KR1020047003708A Expired - Lifetime KR100954641B1 (ko) 2001-09-13 2002-09-12 아미노펩티다아제
KR1020087030860A Expired - Lifetime KR100956907B1 (ko) 2001-09-13 2002-09-12 아미노펩티다아제

Family Applications After (1)

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KR1020087030860A Expired - Lifetime KR100956907B1 (ko) 2001-09-13 2002-09-12 아미노펩티다아제

Country Status (21)

Country Link
US (3) US6921659B2 (enExample)
EP (1) EP1432793B1 (enExample)
JP (2) JP4831932B2 (enExample)
KR (2) KR100954641B1 (enExample)
CN (1) CN100552023C (enExample)
AT (1) ATE440134T1 (enExample)
AU (1) AU2002326884B2 (enExample)
BR (1) BR0212886A (enExample)
CA (1) CA2460309C (enExample)
CY (1) CY1109903T1 (enExample)
DE (1) DE60233417D1 (enExample)
DK (1) DK1432793T3 (enExample)
ES (1) ES2329880T3 (enExample)
HU (3) HU225346B1 (enExample)
IL (2) IL160658A0 (enExample)
MX (1) MXPA04002326A (enExample)
NZ (2) NZ531516A (enExample)
PL (1) PL206286B1 (enExample)
PT (1) PT1432793E (enExample)
WO (1) WO2003023030A2 (enExample)
ZA (1) ZA200401752B (enExample)

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BR0212886A (pt) 2001-09-13 2004-10-13 Genentech Inc Célula bacteriana, método para a produção de um polipeptìdeo heterólogo, método para a prevenção de clivagem, métodos para a clivagem e métodos para a produção de um polipeptìdeo clivado
DK1733037T3 (en) * 2004-03-11 2015-01-12 Genentech Inc Process for the preparation of polypeptides
EP2376619A4 (en) 2008-12-15 2012-07-04 Greenlight Biosciences Inc METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS
KR20110134380A (ko) * 2008-12-22 2011-12-14 그린라이트 바이오사이언시스, 아이엔씨. 화합물의 제조를 위한 조성물 및 방법
CA2797786C (en) 2010-05-07 2020-09-22 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through enzyme relocation
ES2839424T3 (es) 2010-08-31 2021-07-05 Greenlight Biosciences Inc Métodos para control de flujo en vías metabólicas mediante manipulación de proteasas
WO2013036787A2 (en) 2011-09-09 2013-03-14 Greenlight Biosciences, Inc. Cell-free preparation of carbapenems
SG11201600898YA (en) 2013-08-05 2016-03-30 Greenlight Biosciences Inc Engineered proteins with a protease cleavage site
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
EP3277808A4 (en) 2015-03-30 2019-05-22 Greenlight Biosciences, Inc. ACELLULAR PRODUCTION OF RIBONUCLEIC ACID
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
RU2018138975A (ru) 2016-04-06 2020-05-12 Гринлайт Байосайенсис, Инк. Бесклеточная продукция рибонуклеиновой кислоты
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
CN106967658B (zh) * 2017-02-23 2020-09-15 郑州大学 一种提高Fab抗体表达量的方法
WO2019075167A1 (en) 2017-10-11 2019-04-18 Greenlight Biosciences, Inc. METHODS AND COMPOSITIONS FOR THE PRODUCTION OF NUCLEOSIDE TRIPHOSPHATE AND RIBONUCLEIC ACID
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase

Family Cites Families (17)

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DE122004000009I2 (de) * 1984-08-16 2006-09-07 Savient Pharmaceuticals Inc Methode zur Herstellung von menschlichen Wachstumshormonen
WO1986007380A1 (fr) * 1985-06-04 1986-12-18 Takeda Chemical Industries, Ltd. Procede de preparation de polypeptides
US4946783A (en) * 1987-01-30 1990-08-07 President And Fellows Of Harvard College Periplasmic protease mutants of Escherichia coli
WO1988005821A1 (en) 1987-01-30 1988-08-11 President And Fellows Of Harvard College Periplasmic protease mutants of escherichia coli
JPH01181796A (ja) * 1988-01-14 1989-07-19 Ajinomoto Co Inc 組換えｄｎａ法を用いたポリペプチドの製造法
JP2844870B2 (ja) * 1989-07-21 1999-01-13 味の素株式会社 組み替えｄｎａ法によるポリペプチドの製造法
US5508192A (en) * 1990-11-09 1996-04-16 Board Of Regents, The University Of Texas System Bacterial host strains for producing proteolytically sensitive polypeptides
US5304472A (en) * 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
US5573923A (en) * 1993-12-22 1996-11-12 Eli Lilly And Company Method for removing N-terminal dipeptides from precursor polypeptides with immobilized dipeptidylaminopeptidase from dictyostelium discoideum
US6127144A (en) * 1993-12-23 2000-10-03 Cangene Corporation Method for expression of proteins in bacterial host cells
US5639635A (en) * 1994-11-03 1997-06-17 Genentech, Inc. Process for bacterial production of polypeptides
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
KR100369839B1 (ko) * 1997-02-28 2003-06-12 주식회사 엘지생명과학 바실러스리케니포미스균주에서유래한아미노펩티다제,그의제조방법및이를이용한천연형단백질의제조방법
CA2352046A1 (en) * 1998-12-07 2000-06-15 Novozymes A/S Glucoamylases with n-terminal extensions
CN1353755A (zh) * 1999-04-30 2002-06-12 法玛西雅公司 用气单胞菌氨肽酶由多肽除去n末端丙氨酸残基的方法
KR100400638B1 (ko) 2000-12-06 2003-10-08 주식회사 엘지생명과학 재조합 인간 성장호르몬 유사체의 분리방법
BR0212886A (pt) 2001-09-13 2004-10-13 Genentech Inc Célula bacteriana, método para a produção de um polipeptìdeo heterólogo, método para a prevenção de clivagem, métodos para a clivagem e métodos para a produção de um polipeptìdeo clivado

Patent Citations (1)

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US4865974A (en) * 1985-09-20 1989-09-12 Cetus Corporation Bacterial methionine N-terminal peptidase

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Science, Vol. 277, pp.1453~1462 (1997) *
Science,1997, vol 277, pp1453-1462. *

Also Published As

Publication number Publication date
KR20040041611A (ko) 2004-05-17
JP2005502359A (ja) 2005-01-27
AU2002326884B2 (en) 2007-10-18
WO2003023030A2 (en) 2003-03-20
PL206286B1 (pl) 2010-07-30
HU225346B1 (en) 2006-10-28
EP1432793A2 (en) 2004-06-30
HUP0401869A3 (en) 2005-06-28
CY1109903T1 (el) 2014-09-10
NZ531516A (en) 2006-03-31
JP4831932B2 (ja) 2011-12-07
HU0600309D0 (en) 2006-06-28
CA2460309A1 (en) 2003-03-20
DE60233417D1 (en) 2009-10-01
PT1432793E (pt) 2009-11-24
KR100956907B1 (ko) 2010-05-11
ZA200401752B (en) 2005-05-25
US20030124664A1 (en) 2003-07-03
MXPA04002326A (es) 2004-06-29
NZ544964A (en) 2007-08-31
EP1432793B1 (en) 2009-08-19
HU0600310D0 (en) 2006-06-28
US7632658B2 (en) 2009-12-15
CN100552023C (zh) 2009-10-21
DK1432793T3 (da) 2009-12-07
IL160658A (en) 2009-05-04
ATE440134T1 (de) 2009-09-15
JP5372061B2 (ja) 2013-12-18
CN1555412A (zh) 2004-12-15
CA2460309C (en) 2012-07-10
WO2003023030A3 (en) 2003-07-10
BR0212886A (pt) 2004-10-13
US20080160572A1 (en) 2008-07-03
US20060019341A1 (en) 2006-01-26
PL368463A1 (en) 2005-04-04
KR20090018142A (ko) 2009-02-19
HU225430B1 (en) 2006-11-28
EP1432793A4 (en) 2006-05-24
IL160658A0 (en) 2004-08-31
HUP0401869A2 (hu) 2004-12-28
HK1062695A1 (en) 2004-11-19
US7229787B2 (en) 2007-06-12
JP2011177189A (ja) 2011-09-15
ES2329880T3 (es) 2009-12-02
HU225243B1 (en) 2006-08-28
US6921659B2 (en) 2005-07-26

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