KR100863449B1 - Functional cosmetics using citron, phellius linteus, and dongchonnghachopaecilomyces - Google Patents

Abstract

The present invention relates to functional cosmetics mainly composed of citron and cordyceps sinensis mushroom and citrus mushrooms. Citron is freeze-dried citron juice and cordyceps and situation mushrooms are boiled in tertiary distilled water and then concentrated by evaporator to freeze-dry After the lyophilized citron and Cordyceps sinensis mushrooms and mushrooms are made into a powder state, the present invention relates to functional cosmetics having excellent anti-aging and whitening effects of skin cells.

Description

Functional cosmetics using citron, green mushroom, and Cordyceps sinensis {Functional cosmetics using citron, phellius linteus, and dongchonnghacho (paecilomyces)}

The present invention relates to a functional cosmetics mainly containing citron, situation mushroom, cordyceps sinensis. More specifically, the present invention relates to a functional cosmetic having excellent aging inhibitory and whitening effect of skin cells prepared by lyophilizing the filtrate of citron, situation mushroom, and Cordyceps sinensis, and powdering them.

In general, the original function of cosmetics can be said to maintain the homeostasis of the skin. Until now, many researches have been conducted on functional cosmetics that have skin aging and whitening effects, but most of the functional cosmetics currently on the market are composed of pure chemicals.

Among them, AHA, retinol, and collagen have been used as main ingredients of functional cosmetics. However, AHA is a small organic acid, and when used for a long time, it causes irritation to the skin.

The present inventors conducted extensive research to solve the problems of the conventional functional cosmetics as described above, the functional cosmetics prepared using natural fruits such as citron, Cordyceps sinensis, Situation mushroom, skin aging and excellent whitening effect It has been found that the present invention has been completed.

Yuzawa situation mushrooms and Cordyceps sinensis contain a lot of useful ingredients for the human body such as vitamins, minerals, alginic acid, unsaturated fatty acids, melanoma, polar nonpolar lipids, and polysaccharides. In particular, minerals, vitamins, unsaturated fatty acids, melanomas, and polysaccharides can be found to inhibit the synthesis of harmful free radicals derived from aging of human skin cells, and are effective in anticancer activity and have excellent whitening effects. there was.

In particular, it was found that the activity of the experimental cell lines fibroblast (fibroblast) and melanoma used in the present invention were mixed with citron, situation mushroom, Cordyceps sinensis, and the activity was increased.

In other words, the result of testing the mixture of citron and situation mushroom, Cordyceps sinensis, inhibited the aging of skin cells and showed an excellent effect on the whitening effect.

Citron, the raw material of the functional cosmetics according to the present invention, which can obtain such an effect, is juiced from 3 months to 4 months of age, and centrifuged at 6000 × g (g: gravity) for 15 minutes, and then filtered by general gauze. Citron juice was lyophilized at -65 ° C.

Sichuan mushrooms and Cordyceps sinensis were obtained by boiling 100 g in 1200 ml of tertiary distilled water for 2 hours and 30 minutes, firstly filtering with regular gauze, centrifuging for 15 minutes at 6000 × g centrifugal force, and secondly filtering with 3 mm thick paper. The supernatant was concentrated on an evaporator at 40 ° C. and then lyophilized at −65 ° C.

The skin aging inhibitory effect and the whitening effect according to the present invention were tested as follows.

Experimental Example 1

Freeze-dried raw materials are pulverized into powder below 250㎛ and 100%, 50, 25, 12.5, 6.25, 3.125, 1.5625, 0.78125, 0.390625, 0.1953125, 0.09765625, MTT (-(4,5-dimethyl-), which can measure the activity of live cells by staining live cells with 12 groups of fibroblasts at a concentration of 0.048828125 mg / ml. The effect of inhibiting skin aging was evaluated by measuring the absorbance at 560 nm, the optimum absorption wavelength, of 2-thiazolyl) -2,5-diphenyl-2H-tetrazoliumbromide) assay. In addition, the whitening effect was evaluated by treating with 12 groups of murine melanomas and measuring the amount of melanin, a component that inhibits the whitening effect with absorbance at 475 nm, which is the optimum absorption wavelength of the melanin pigment.

The results are shown in Table 1.

The results of MTT analysis of fibroblast showed that high activity was obtained when the raw material of the present invention was treated at a concentration of 12.5-0.953125 mg / ml.

In addition, as a result of measuring the amount of melanin produced by the raw material of the present invention, it was confirmed that the highest activity, that is, the production of melanin was inhibited at 25.0 to 1.5625 mg / ml.

TABLE 1

Concentration of raw material (mg / ml) MTT analysis Melanin absorbance 0 0.021 ± 0.001 1.023 ± 0.003 100 0.044 ± 0.004 0.831 ± 0.002 50 0.041 ± 0.003 0.731 ± 0.002 25 0.078 ± 0.002 0.213 ± 0.001 12.5 0.123 ± 0.005 0.124 ± 0.004 6.25 0.126 ± 0.001 0.098 ± 0.003 3.125 0.138 ± 0.001 0.112 ± 0.001 1.5625 0.174 ± 0.001 0.108 ± 0.001 0.78125 0.115 ± 0.006 0.547 ± 0.003 0.390625 0.117 ± 0.002 0.547 ± 0.002 0.1953125 0.121 ± 0.002 0.611 ± 0.003 0.09765625 0.041 ± 0.001 0.781 ± 0.001 0.048828125 0.036 ± 0.007 0.991 ± 0.001

As can be seen from Table 1, the functional cosmetics of the present invention is the most excellent skin cell aging inhibitory effect when prepared in a concentration of 12.5 ~ 0.1953125 mg / ml of lyophilized citron and situation mushroom, Cordyceps sinensis in 0.8% methanol When prepared in a concentration of 25.0 ~ 1.5625 mg / ml, it showed the most excellent whitening effect.

Experimental Example 2

In order to confirm the excellent effect of the functional cosmetic of the present invention, it was tested in comparison with the existing functional cosmetics.

Liposome and cyclodextrin complexes, known as raw materials of citron and Cordyceps sinensis, raw mushroom and commercial functional cosmetics, which are the raw materials of the present invention, are group 12 at concentrations of 15, 9, 6, 3 and 1 mg / ml, respectively. Fibroblast's and murine melanomas of 12 groups were treated to measure the activity at the same absorbance as described above and are shown in Table 2.

TABLE 2

Concentration of raw material (mg / ml)                                          Citron, Cordyceps sinensis, Situation mushrooms as raw materials of the present invention Liposome Cyclodextrin complex MTT analysis Melanin analysis MTT analysis Melanin analysis MTT analysis Melanin analysis 15.0 0.134 ± 0.001 0.122 ± 0.001 0.124 ± 0.002 0.183 ± 0.003 0.121 ± 0.002 0.128 ± 0.001 9.0 0.128 ± 0.002 0.127 ± 0.002 0.119 ± 0.001 0.148 ± 0.003 0.098 ± 0.002 0.168 ± 0.003 6.0 0.137 ± 0.001 0.105 ± 0.001 0.099 ± 0.003 0.198 ± 0.003 0.107 ± 0.004 0.201 ± 0.001 3.0 0.131 ± 0.003 0.102 ± 0.004 0.117 ± 0.001 0.205 ± 0.001 0.087 ± 0.005 0.188 ± 0.004 1.0 0.129 ± 0.003 0.118 ± 0.003 0.089 ± 0.001 0.214 ± 0.003 0.058 ± 0.001 0.187 ± 0.003

As shown in Table 2, looking at the activity of each raw material, it was found that the yuzu, Cordyceps sinensis, the situation mushroom, the raw material of the present invention is superior to the liposome and cyclodextrin complex.

In other words, looking at the results of the MTT analysis, the present invention can be confirmed that the higher than the level of the liposomes and cyclodextrin complex, thereby the functional cosmetics of the present invention was found to have excellent skin anti-aging effect.

On the other hand, looking at the results of the melanin analysis, it can be seen that the present invention is lower than the values of the liposomes and cyclodextrin complex, whereby the functional cosmetics of the present invention showed an excellent whitening effect.

As described above, the functional cosmetics according to the present invention, unlike the cosmetics by the conventional pure chemicals, by using the natural fruit citron and herbal medicine situation mushrooms, Cordyceps sinensis, showing excellent anti-aging effect and excellent whitening effect of skin cells.

Claims (2)

Aged 3 months to 4 months old citron juice, centrifuged at 6000 × g of centrifugal force, filtered with gauze to obtain a citron juice solution, lyophilized, 100 g each of mushrooms and cordyceps in 1200 ml of tertiary distilled water for 2 hours 30 minutes. After boiling for 1 hour, the filtrate was first filtered, centrifuged at 6000 × g of centrifugal force, and the supernatant obtained by secondary filtration with 3 mm thick paper was concentrated by an evaporator, and then lyophilized. The powder was made with a pulverizer, and then the same amount of powder of citron, mushrooms and Cordyceps sinensis in 0.8% methanol was prepared in a concentration of 12.5 ~ 0.1953125 mg / ml functional cosmetics for inhibiting skin cell aging.  Aged 3 months to 4 months old citron juice, centrifuged at 6000 × g of centrifugal force, filtered with gauze to obtain a citron juice solution, lyophilized, 100 g each of mushrooms and cordyceps in 1200 ml of tertiary distilled water for 2 hours 30 minutes. After boiling for 1 hour, the filtrate was first filtered through gauze, centrifuged at 6000 × g centrifugal force, and then, the supernatant obtained by secondary filtration with 3 mm thick paper was concentrated with an evaporator, and then lyophilized. Whitening functional cosmetics prepared in a concentration of 25 ~ 1.5625 mg / ml by mixing the same amount of powder of citron, mushrooms and Cordyceps sinensis in 0.8% methanol.