KR100345382B1 - Whitening cosmetic material containing an extract of natural Phellinus Linteus - Google Patents

Abstract

The present invention relates to a whitening cosmetic containing natural mushroom extract, more particularly, to form a natural mushroom mushroom having a predetermined strength as a fine powder, ultra-high frequency treatment of the natural mushroom mushroom powder under a reduced pressure in a specific solvent At the same time, after sterilization, discoloration, and odor removal, the extracts of natural situation mushrooms can be efficiently obtained, and the natural situation mushroom extracts contain at least one ultraviolet absorber or ultraviolet scattering agent and one or more peptides that inhibit the synthesis of melanin. , Whitening cosmetics containing licorice extract, arbutin, rice extract, vitamin C, etc. to prevent blackening or pigmentation of the skin by ultraviolet rays and inhibit melanin production such as skin to improve skin tone, blemish and freckles Of course, natural situation mushroom extract to have an anti-aging function due to the antioxidant effect It relates to a whitening cosmetic containing water.

To this end, the present invention is pulverized natural situation mushroom having a strength of 0.5 ~ 0.8N / ㎜ and formed into a powder, the natural situation obtained by ultra-high frequency treatment under a reduced pressure in a specific solvent, decolorization and odor removal In the mushroom extract, 20 parts by weight of the natural mushroom extract, 3.0 parts by weight of cetostearyl alcohol, 1.0 parts by weight of beeswax, 3.0 parts by weight of self-emulsifying monostearate glycerol, 1.0 parts by weight of lysosorbate 60, 0.4 parts by weight of non-stearate, 1.6 parts by weight of preservative, 5.0 parts by weight of sulfate agent, 10 parts by weight of mineral oil, 5.0 parts by weight of squalane, 5.0 parts by weight of sodium hyaluronate, 5.0 parts by weight of propylene glycol, purified water 40 It is characterized by consisting of parts by weight.

Description

Whitening cosmetic material containing an extract of natural Phellinus Linteus

The present invention relates to a whitening cosmetic containing natural mushroom extract, more particularly, to form a natural mushroom mushroom having a predetermined strength as a fine powder, ultra-high frequency treatment of the natural mushroom mushroom powder under a reduced pressure in a specific solvent At the same time, after sterilization, discoloration, and odor removal, the extracts of natural situation mushrooms can be efficiently obtained, and the natural situation mushroom extracts contain at least one ultraviolet absorber or ultraviolet scattering agent and one or more peptides that inhibit the synthesis of melanin. Whitening cosmetics containing licorice extract, arbutin, rice extract, vitamin C, etc. to prevent blackening or pigmentation of the skin by ultraviolet rays and to suppress melanin production such as skin to improve skin tone, blemish and freckles Of course, natural situation mushroom extract to have an anti-aging function due to the antioxidant effect This invention relates to a containing whitening cosmetic.

In general, the situation mushroom (桑 黄) mushrooms in China, also known as different (桑 耳) has been investigated as a parasitic to the mulberry tree, but according to the survey in Korea, in addition to the mulberry tree, some of the hardwoods in the heart of the forest can be seen that wild It is known as (Dongyangryul, Monthly Damp, P34-37, 1992.12), and belongs to the Phellius Quel.em.lmaz of the Hemenochaetaceae. ).

About 48 species of wood mud mushrooms in the world have been reported, but in Korea, mud mushrooms (p. Ferrginosus), dried mud mushrooms (P. gilvus), horse mud mushrooms (P. igniarius) and eggplant mud mushrooms (P. laevigatus) ), Prunus mud mushroom (P. pomaceus), clay mud mushroom (P. robustus), fir mud mushroom, deciduous mud mushroom (P. pini), etc. There are 8 species, and pharmacological effects include anticancer effect, immune enhancing function, It is known to be effective in treating gynecological diseases such as tumor suppression effect, uterine bleeding and menstrual irregularities.

In addition, natural situation mushroom extract contains a large amount of various sugars, flavonoids, fiber, isoflavones, etc., so it is excellent in moisturizing, anti-aging, and antioxidant effects in cosmetics, especially in terms of skin whitening effect. Reported to be excellent [T. lked, T. T sutsumi, Fragrance J., 6, 59 (1990)], and skin aging is known to play an important role in physiological aging and photoaging. Reactive Oxygen Species, Free Radical, alters biomolecules such as lipids, proteins and nucleic acids, resulting in aging (Harman, 1956; 1992; Richter et al., 1988: Oliver et al. , 1987; Brank et al., 1992)

These reactive oxygen species are also produced during normal metabolic processes, and can be produced in excess when diseased or stressed. In particular, the skin is exposed to ultraviolet rays, and photochemical reactions that produce reactive oxygen species continue to occur. In addition, smoking, pollution and bacterial infections also produce reactive oxygen species, and excess reactive oxygen species endanger the antioxidant defenses of the skin, leading to lipid peroxidation and DNA oxidation, resulting in pathological cancer and reduced skin elasticity, wrinkles and freckles. This leads to skin aging, which can be said to delay the aging of skin when the skin sulphation defense is strengthened by continuous supplementation of antioxidants through foods or cosmetics containing the necessary antioxidants.

Therefore, cosmetics to prevent the formation of blemishes, freckles, and remove the pigments deposited must be prescribed in combination with a substance that inhibits the production and production of melanin, a sunscreen, a moisturizer for preventing skin aging, and an metabolic accelerator.

Therefore, in recent years, products containing situational mushroom extracts have been handled in pharmaceuticals and cosmetics fields, and excellent whitening and sulfated effects have been demonstrated. However, most of them use artificially cultivated situational mushrooms with very few beneficial ingredients to humans, and also artificial cultures. Extracts of cultivated mushrooms are grown in a water-soluble water bath, which is a common method, and thus have low extraction efficiency and have a gentle effect on the prevention level of melanin pigmentation and the prevention and healing of blemishes and freckles that may be caused by UV light. It has a disadvantage.

In other words, the conventional method of extracting artificial mushrooms and extracts and cosmetics using the extracts are pulverized artificial mushrooms with little strength as shown in Korean Patent Publication No. 98-50697 (published date 1998.09.15) After crushing the mashed mushrooms in water, the method of water extraction, the ethanol extraction method by boiling the mashed mushrooms in 60-95% ethanol, and the mashed mushrooms The extract is obtained by a conventional method, such as a mixed solution extraction method obtained by treating a mushroom with a mixed solution of 1.3-butylene glycol and water.

However, the situation mushroom grown by such artificial cultivation contains not only a few contents of sugars, flavonoids, fiber, isoflavones, etc., which are much lower than those of natural cultivated mushrooms. When used in cosmetics, virtually no inherent effect can be obtained, and natural situation mushrooms have a strength of about 0.5 to 0.8 N / mm2 compared to the artificial culture situation mushrooms, and it is virtually impossible to obtain extracts by the above-described water bath method. Will be done.

Another conventional method is to use an extract obtained from the mycelium of the situation mushroom or its culture solution, as in Republic of Korea Patent Publication No. 98-38284 (published date 1998.08.05).

That is, Korean Patent Application Publication No. 98-38284 discloses a situation mycelium or an extract obtained from the culture solution thereof, which is obtained by adding distilled water to the mycelium and removing the hydrothermal extract or mycelium from the hydrothermal extract for about 3 hours at about 100 ° C. using a superfiltration membrane having a molecular weight of 10,000. Filtered to remove low molecular weight substances with a molecular weight of 10,000 or less, and then precipitated by the addition of alcohol to obtain a precipitate obtained by centrifugation. This mycelium is used as a cosmetic composition.

However, the conventional Republic of Korea Patent Publication No. 98-38284 has the efficacy of pharmacological action, such as anti-cancer effect, immune enhancing function, tumor suppression effect, uterine bleeding, menstrual irregularity, compared to natural situation mushroom by cultivating mycelium of situation mushroom There is a remarkably low problem.

In order to solve the above problems, the content of various kinds of sugars and components such as flavonoids, fiber, isoflavone, etc. is much higher than artificial cultivated mushrooms and has a strength of about 0.5 to 0.8 N / mm2. Mushrooms are used, but the above-mentioned natural situation mushroom is mechanically pulverized to form a fine powder, and the pulverized natural situation mushroom powder is prepared by using a solvent such as ethanol aqueous solution, 1.3-butylene glycol, propylene glycol, isopropanol, etc. Natural situation mushroom extract obtained by ultra-high frequency treatment under reduced pressure at 65 ° C and filtered with activated carbon, at least one ultraviolet absorber or ultraviolet scattering agent, peptide, licorice extract, arbutin, rice extract, vitamin C which inhibit the synthesis of at least one melanin A whitening cosmetic compound containing the back is prepared to prevent skin blackening or pigmentation by ultraviolet rays. It is a technical problem to prevent and inhibit the production of melanin, such as skin, to improve skin tone, blemish and freckles, as well as to prevent skin aging due to antioxidant effects.

The present invention is a natural situation mushroom extract having a strength of 0.5 ~ 0.8N / ㎜ crushed to form a powder, the natural situation mushroom powder obtained by depressurization and deodorization after ultra-high frequency treatment under reduced pressure in a specific solvent 20 parts by weight of the natural situation mushroom extract, 3.0 parts by weight of cetostearyl alcohol, 1.0 part by weight of beeswax, 3.0 parts by weight of self-emulsifying monostearate monoglyceride, 1.0 part by weight of lysosorbate 60, sorbitan stearate 0.4 parts by weight, 1.6 parts by weight of preservative, 5.0 parts by weight of sulfate agent, 10 parts by weight of mineral oil, 5.0 parts by weight of squalane, 5.0 parts by weight of sodium hyaluronate, 5.0 parts by weight of propylene glycol, and 40 parts by weight of purified water. It is characterized by what has been done.

1 is an extraction process of the natural situation mushroom extract used in the present invention.

Figure 2 is an ultraviolet (UV) spectrum diagram of the natural situation mushroom extract used in the present invention

Figure 3 is a graph showing the results of the melanin inhibition of the production of natural situation mushroom extract used in the present invention.

Figure 4 is a graph showing the results of the skin color improvement effect of the whitening cosmetics containing natural situation mushroom extract according to the present invention.

Hereinafter, the whitening cosmetics containing the natural situation mushroom extract according to the present invention will be described in detail with reference to FIGS. 1 to 4.

1 is an extraction process of the natural situation mushroom extract used in the present invention, first performed a washing process (1) to remove foreign matter by washing the natural situation mushroom having a strength of about 0.5 ~ 0.8 N / ㎜ in clean water Next, drying the washed natural situation mushrooms at room temperature 20 ~ 25 ℃ blocked sunlight and dried for about 1 to 2 months in a well-ventilated condition, almost no change in weight, that is, dry to dry less than 5% moisture Proceed to step (2).

Through the drying step (2) above, the natural situation mushrooms dried to 5% or less of moisture are firstly pulverized to a size of about 5 to 15 mm using a pulverizer, and then the primary pulverized natural situation mushrooms are precision grinders (Henshel). Mixer AM-7, Japan) is subjected to a pulverization step (3) of secondary milling and grinding into particles of about 8-12 meshes to form naturally occurring mushroom powder.

The natural situation mushroom powder formed from 8 to 12 mesh particles obtained through the crushing step (3) and an aqueous solution of 30% 1,3-butylene glycol as an extraction solvent were mixed at a ratio of 1: 3, and then 0.6 to 0.8 kgf. The ultra-high frequency treatment step (5), which supplies ultra-high frequency for about 24 hours in the course of the decompression bath (4), is carried out while the decompression bath is left for 48 hours at 55-65 ° C. under reduced pressure / cm 2. Run

In this case, the pressure-reduced jungtang is a mixture of natural mushroom powder and 30% 1,3-butylene glycol aqueous solution, which is an extraction solvent, and then heated to uniformly and uniformly heat the mixture without heating the mixture to heat it. Bath) means indirect heating, and the reason for supplying ultra-high frequency in the decompression bath (4) is to raise the efficiency of extraction by generating heat to the natural mushroom powder and extraction solvent when supplying the ultra-high frequency during the bath. It is to increase the whitening effect.

After completing the vacuum bath (4) and the ultra-high frequency treatment step (5), the filter paper was passed through a filter to firstly filter powder particles and impurities of natural mushrooms, and then secondly filter through a 0.45 μm bacteria filter paper. After performing the filtration and sterilization process (6) to remove the bacteria, and through the activated carbon column to proceed through the off-odor removal and deodorization and deodorization process (7) to complete the production of natural situation mushroom extract.

The following describes another embodiment in the extraction process of the natural situation mushroom extract used in the present invention is as follows, washing step (1), drying step (2), grinding step (3), ultra-high frequency treatment in the whole process The step (5), the filtration and sterilization step (6), the odor and deodorization step (7) are the same as the above-described steps, and only the reduced pressure bath (4) will be differently described.

(Example 1)

As described above, the natural situation mushroom powder formed of 8-12 mesh particles obtained by sequentially performing the drying step (2) and the grinding step (3) and the 30% ethanol aqueous solution as an extraction solvent are mixed at a ratio of 1: 3. After leaving for 5 days to perform a vacuum bath (4) to evaporate the ethanol while boiling at 55 ~ 65 ℃ under 0.6 ~ 0.8kgf / ㎠ pressure reduction and then filtration and sterilization process (6) and odor and deodorization process (7) Proceed in turn.

(Example 2)

As described above, the natural situation mushroom powder formed from 8 to 12 mesh particles obtained by sequentially performing the drying step (2) and the grinding step (3) and the 30% propylene glycol aqueous solution as an extraction solvent in a ratio of 1: 3. After mixing for 48 hours, the process was carried out under reduced pressure bath (4), which was bathed at 55-65 ° C. under reduced pressure of 0.6 to 0.8 kgf / cm 2, followed by filtration and sterilization (6) followed by odor and deodorization (7). do.

(Example 3)

As described above, the mixture of natural situation mushroom powder formed from 8 to 12 mesh particles obtained by sequentially performing the drying step (2) and the grinding step (3) and distilled water, which is an extraction solvent, is mixed at a ratio of 1: 4 for 48 hours. After standing, the pressure-reducing hot water step (4) is carried out in a water bath at 75-85 ° C. under a reduced pressure of 0.6-0.8kgf / cm 2, followed by a filtration and sterilization step (6) and a odor and deodorization step (7).

(Example 4)

As described above, 20 parts by weight of ethanol, 20 parts by weight of 1.3-butylene glycol, and propylene of the natural situation mushroom powder formed of 8 to 12 mesh particles obtained by sequentially performing the drying step (2) and the grinding step (3). 30 parts by weight of glycol, 20 parts by weight of isopropanol, 20 parts by weight of distilled water and a mixed solvent with a ratio of 1: 3, and left for 48 hours, and then depressurized and heated at 55 to 65 ℃ under 0.6 ~ 0.8kgf / ㎠ pressure After performing (4), the filtration and sterilization process (6) and the odor and deodorization process (7) are performed in this order.

As a result of the ultraviolet absorption spectrum analysis of the natural situation mushroom extract extracted by the method of Example 1 to Example 4, as shown in Figure 2 had a strong ultraviolet absorption capacity (190nm-400nm), for application as a skin cosmetic The test showed a very low irritation value (0.80) compared with the general organic UV blocker (stimulation level 2.0), and the antioxidant effect (94.7%) using liroleic acid was artificially cultivated as a control (79.8%), vitamin E (43.3%), green tea extract (83.1%), carrot extract (17.0%) showed an excellent effect.

Also, the free radical capture effect (87.10%) by DPPH method was higher than ginkgo biloba extract (69.5%) and vitamin E (36.5%), and the whitening efficacy test tyrosine inhibitory effect and the ear of C57BL Mouse Inhibition of melanin production using the part, inhibiting melanin production of microorganisms (Streptomyces bikiniensis), whitening effect than natural situation mushrooms, vitamin C, arbutin, kojic acid used as a control group in clinical tests, as well as inhibiting photoaging by UV protection Appeared.

As described above, the whitening effect of the natural situation mushroom extract extracted by the method of Examples 1 to 4, UV absorbing ability, inhibitory effect and disappearance of melanogenesis after UV induction, inhibition of melanin production, antioxidant effect and DPPH free radical inhibition The ability to measure and explain the results are as follows.

Measurement Example 1

Measurement Example 1 is a comparison of the degree of functional inhibition of tyrosinase enzyme to measure the whitening effect of the natural situation mushroom extract extracted by the method of Examples 1 and 4 described above, Cinases are enzymes that activate melanin by activating tyrosine in pigmented cells.

This measurement was carried out according to the experimental methods of Horowits and A. Vanni, etc. As a control, wild mushrooms (volume% of distilled water of 0.01, 0.50, 0.10, 1.00, 10.00), oil-soluble vitamins (0.01, 0.50, 0.10, 1.00, Volume% to distilled water of 10.00), arbutin (vol% to distilled water of 0.01, 0.50, 0.10, 1.00, 10.00), kojic acid (% by volume of distilled water of 0.01, 0.50, 0.10, 1.00, 10.00).

Here, tyrosinase inhibitory activity was measured by placing 0.9 ml of the sample in a microplate (96 wells), adding 0.1 ml of 0.1 molar phosphate buffer solution (pH 6.5), and 0.3 mg / ml L-tyrosine solution, followed by tyrosinase (Sigrma, 1250 U / 0.1 ml of ml mushroom tyrosinase) was added and reacted at 37 ° C. for 10 minutes, and the absorbance was measured at 475 nm using a microplate.

Tyrosinase inhibition rate was calculated by the following equation, IC ₅₀ is the inhibitory concentration of 50% enzyme activity inhibition unit is mg / ml.

Inhibitory concentration (IC ₅₀ ) = (absorbance of sample added / absorbance without sample) × 100

As a result of the test for inhibiting tyrosinase activity under such conditions, the inhibition rate and the concentration IC ₅₀ of each extract of wild situation mushroom showed excellent whitening effect as shown in Table 1 below.

Table 1

Measurement Example 2

Measurement Example 2 is the ultraviolet spectrum (190-700nm) to examine the ultraviolet absorption capacity as shown in Figure 3 using the natural situation mushroom extract extracted by the method of the above-described Examples and Examples 1 to 4 above. As shown in FIG. 3, the effective ultraviolet absorption at the wavelength of 190-400 nm was observed.

Measurement Example 3

Measurement Example 3 is to determine the melanin production inhibitory effect and the degree of disappearance after UV induction to determine the whitening effect using the natural situation mushroom extract extracted by the method of Example 1 and Examples 1 to 4 described above It was for the purpose of seeing.

The experiment was performed by irradiating C57BL Mouse (8W, ♀) with ultraviolet light (FL20-SE lamp) at 15.2mJ / ㎠, once a day for 5 days, and taking ear parts one week after the last irradiation. After removal, the exfoliated skin was immersed in 2N-NaBr solution at 37 ° C. for 2 hours, and then the epidermis was peeled from the dermis to adjust 0.14% L-DOPA solution (precisely to pH 7.2 with 0.1 M phosphate buffer). 1 ml of the test substance was added to 2 ml, and the peeled epidermis was immersed at 37 ° C. for 3 hours to stain melanocytes (DOPA staining).

This determination was performed by measuring the number of melanocytes of 1 mm 2 under a microscope and calculating the average of the number of cells per mm 2, and then calculating the inhibition rate compared with the blank without adding any sample. As shown in Fig. 3, the melanin inhibitory effect and pigmentation loss rate were higher than those of kojic acid and arbutin.

Measurement Example 4

Measurement Example 4 is a strain used to measure the melanin production inhibitory activity against actinomycetes using the natural situation mushroom extract extracted by the method of Examples and Examples 1 to 4 described above is Streptomyces bikiniensis (NRRL B-1049, KCTC-9172).

S. bikiniensis NRRL B-1049 <KCTC-9172> was cultured in Prpavizas VDYA agarslant medium (V-8 juice 200ml, glucose 2g, Yeast EX. 2g, CaCO3 1g, Agar 20g, D-Water 800ml, pH = 7.2) After incubation for 2 weeks to produce spores, spore suspension was made with sterile water, and ISP No. 7 0.2 ml each of the spore suspension was applied to the plate medium, and then the amount of the sample was applied to the paper disk soaked on the surface of the medium with 30 ㎍ / paper disc and incubated at 28 ℃.

The inhibition of melanogenesis was observed in Table 2 using the 4-hydroxyanisole known as the melanogenesis inhibitor as a control, and the results of the ultra-high frequency extraction method according to the present invention were measured. Natural situation mushroom extract extracted by showed a safe and excellent melanin production inhibitory effect.

Table 2

Measurement Example 5

Measurement Example 5 measured the antioxidant power using the natural situation mushroom extract extracted by the method of Example 1 and Examples 1 to 4 described above, by using linolenic acid which is a good automatic oxidation material Inhibition of Autooxidation and Lipid Peroxidation on Funita et. al is DPdee free radical scavenging effect.

Antioxidant effect was obtained by adding 2mg / ml linolenic acid, 10mg / ml Tween 20, 0.2M phosphate buffer (pH 7.4), and sample to make 0.1ml, and then incubating at 37 ℃ for 24 hours, 80% ethanol 0.1ml, 30% 0.1 ml of ammonium thiocyanate and 20 ml of ferrous ammonium sulfate-3.5% hydrochloric acid were added, and after 3 minutes, the absorption wavelength was measured at 500 nm, and the antioxidant effect was measured in% compared to the control without the antioxidant effect sample.

The inhibitory effect of DPPH free radical was determined by measuring the absorbance of 0.1mlM DPPH Methanolic acid 1.0ml and 1.0ml Ethanolic sample at 516nm after 30 minutes at 37 ° C.

That is, as can be seen in Table 3, the natural situation mushroom extract according to the method of the present invention showed both excellent antioxidant effect and free radical inhibitory effect on linolenic acid.

Table 3

The following is to prepare a whitening cosmetics according to the present invention by using the situation mushroom extract extracted by the method of one embodiment and Examples 1 to 4 described above, the whitening cosmetic composition is generally added to the cosmetic base used in general According to the cosmetic preparation method it can be prepared in cosmetics such as lotion, milky lotion, cream.

The whitening cosmetics containing such natural mushroom extracts, (a) 0.10 ~ 40.0 parts by weight of the natural mushroom extract high concentration extracted by the method of the above-described embodiments and Examples 1 to 4, (b) octylmeth 0.1 to 5.0 parts by weight of oxy cinnamate, 0.1 to 5.0 parts by weight of benzophenone, 0.1 to 5.0 parts by weight of oxybenzone, (c) 0.10 to 30.0 parts by weight of melanin synthesis inhibitory peptide, 0.1 to 30.0 parts by weight of licorice extract, Arbutin 0.1-30.0 weight part, Rice extract 0.1-30.0 weight part Cosmetics containing 1 or more types.

Among these, the component (b) and the component (c) are not limited to one or two or more kinds thereof, and the component (b) has a problem in the feeling of use, stability and stability of the cosmetics when it is added in an amount of 5.0 parts by weight or more. It is preferable to mix | blend in 5.0 weight part or less.

As described above, the whitening cosmetic of the present invention containing the natural situation mushroom extract according to the present invention is characterized in that the above-mentioned ingredients (a), (b) and (c) are blended. You may mix base and various additives. For example, an oil, an activator, a fragrance, a preservative, etc. are mix | blended.

Hereinafter, the whitening cosmetics of the present invention will be described in more detail with reference to Examples 5 to 8, and these examples are merely illustrative of the present invention, and the scope of the present invention is not limited to these examples.

Example 5 and Example 6 below are to prepare a whitening cream using the natural mushroom extract extracted by the method of the above-described Examples and Examples 1 to 4.

(Example 5)

20 parts by weight of natural situation mushroom extract, 3.0 parts by weight of cetostearyl alcohol, 1.0 parts by weight of beeswax, 3.0 parts by weight of self-emulsifying monostearate, 1.0 parts by weight of lysosorbate 60, 0.4 parts by weight of sorbitan stearate And white parts by mixing 1.6 parts by weight of preservative, 5.0 parts by weight of sulfate agent, 10 parts by weight of mineral oil, 5.0 parts by weight of squalane, 5.0 parts by weight of sodium hyaluronate, 5.0 parts by weight of propylene glycol, and 40 parts by weight of purified water. Cream was prepared.

(Example 6)

18 parts by weight of natural situation mushroom extract, 3.0 parts by weight of cetostearyl alcohol, 1.0 part by weight of beeswax, 3.0 parts by weight of self-emulsifying monostearate monoglyceride, 1.0 part by weight of lysosorbate 60, 0.4 weight of sorbitan stearate 10 parts by weight of mineral oil, 5.0 parts by weight of squalane, 5.0 parts by weight of sodium hyaluronate, 5.0 parts by weight of propylene glycol, 1.6 parts by weight of sulfated agent, 0.2 parts by weight of benzophenone, 0.3 parts by weight of oxybenzone, , 0.5 parts by weight of octyl methoxycinnamate, 30 parts by weight of purified water, 5.0 parts by weight of melanin inhibiting peptide, 5.0 parts by weight of licorice extract, 1.0 parts by weight of arbutin, and 5.0 parts by weight of rice extract to prepare a whitening cream Was

Table 4 shows the compositions and mixing ratios of the whitening creams prepared in Examples 5 and 6.

Table 4

In the preparation of the whitening cream in Examples 5 and 6 above, after dissolving the first phase and the second phase uniformly at 75-85 ° C. in a separate container, the first phase is added to the second phase, The mixture was emulsified by stirring at 3600rpm for 5 minutes with a homomixer at 25rpm for 20 minutes with a paddle mixer, and the obtained mixture was cooled again to 40-50 ° C. and a third phase was added thereto, followed by stirring at 25 rpm with a paddle mixer again and then cooled to 25 ° C. To prepare a whitening cream.

In Examples 7 and 8 below, the whitening cosmetics were prepared by using the natural situation mushroom extract extracted by the method of Examples 1 and 4 described above.

(Example 7)

5.0 parts by weight of natural situation mushroom extract, 5.0 parts by weight of sodium hyaluronate, 5.0 parts by weight of propylene glycol, 5.0 parts by weight of glycerin, 0.05 parts by weight of disodium ethylenediamine tetraacetate, 75 parts by weight of purified water, 3.0 parts by weight of ethanol And 1.25 parts by weight of antioxidant and 0.7 parts by weight of polyoxyethylene hydrogenated castor oil were mixed to prepare a whitening cosmetic.

(Example 8)

5.0 parts by weight of natural situation mushroom extract, 5.0 parts by weight of sodium hyaluronate, 5.0 parts by weight of propylene glycol, 5.0 parts by weight of glycerine, 0.05 parts by weight of disodium ethylenediamine tetraacetate, 3.0 parts by weight of ethanol, and polyoxyethylene curing 0.7 parts by weight of castor oil, 59 parts by weight of purified water, 0.2 parts by weight of oxybenzone, 0.5 parts by weight of octylmethoxy cinnamate, 5.0 parts by weight of melanin inhibitor, 5.0 parts by weight of licorice extract, 1.0 parts by weight of arbutin And, 5.0 parts by weight of rice extract, 0.55 parts by weight of antioxidant was mixed to prepare a whitening cosmetics.

The composition and the mixing ratio of the whitening water prepared by Examples 7 and 8 are shown in Table 5.

Table 5

In the preparation of the whitening cosmetics in Examples 7 and 8, the first and second phases were homogenized, respectively, at room temperature, and then the second phase was gradually added while stirring the first phase with a stirring rod, respectively. Prepared.

Skin whitening cream obtained from Examples 5 and 6 prepared with such ingredients and mixing ratios, using natural situation mushroom extract, melanin synthesis inhibitor, licorice extract, arbutin, rice extract, and sunscreen freckles with whitening cream containing sunscreen In order to determine the effect as a light control (L value) was calculated using a color difference meter (Minorta, Chromameter C R-300) for the Well Control Test according to the double blind method.

Experimental method was irradiated with UV8 0.88J / ㎠, once a day / three times a day, the sample was applied twice a day for 30 days, the whitening effect according to the L value before and after application and the passage of time Accordingly, ΔL value, which is a difference from the L value of the surrounding normal region, is obtained, and the disappearance rate of pigmentation is compared with FIG. 4.

The whitening effect is calculated as L (%) = | (L value before application-L value after application) / L value before application | × 100. According to Table 6, natural mushroom extracts, other whitening effect ingredients, and sunscreen are combined together. It can be seen that it is used not only to prevent blackening of the skin but also to remove skin stains, leading to an excellent synergistic effect on the whitening effect.

Table 6

In addition, Table 7 is a result of measuring the antioxidant effect on the whitening longevity made by the same ingredients and manufacturing method as in Example 7 and Example 8, as in this result, the Example has a good antioxidant effect and a free radical inhibitory effect You can see that.

Table 7

As in Examples 5 to 6, the whitening cosmetics containing natural situation mushroom extract showed an excellent effect as a complex synergy of skin aging prevention and whitening effect by effective UV blocking.

As described above, the present invention has a natural content of various kinds of sugars, flavonoids, fibers, isoflavones, and the like, which are much higher than artificial cultured mushrooms and have a strength of about 0.5 to 0.8 N / mm 2. The above-mentioned natural situation mushroom is formed into a fine powder, and the pulverized natural situation mushroom powder is subjected to ultra-high frequency under reduced pressure at 55-65 ° C. using a solvent such as ethanol aqueous solution, 1.3-butylene glycol, propylene glycol, isopropanol, etc. Whitening cosmetics containing a mixture of one or more ultraviolet absorbers or ultraviolet scattering agents, peptides, licorice extracts, arbutin, rice extracts, vitamin C, etc., which inhibit the synthesis of at least one melanin to natural extracts of natural situation mushrooms obtained by filtration with activated carbon. To prevent blackening or pigmentation of the skin by ultraviolet rays and to produce melanin such as skin And also improve the tone and blemishes, freckles and skin, of course, is that you can achieve the effect of skin aging due to the antioxidant effect.

Claims (4)

In the natural situation mushroom extract obtained by pulverizing the natural situation mushroom having a strength of 0.5 ~ 0.8N / ㎜ and powdered, the natural situation mushroom powder obtained by ultra-high frequency treatment under reduced pressure in a specific solvent, decolorization and odor removal, 20 parts by weight of the natural mushroom extract, 3.0 parts by weight of cetostearyl alcohol, 1.0 parts by weight of beeswax, 3.0 parts by weight of self-emulsifying monostearate, 1.0 parts by weight of lysosorbate 60, sorbitan stearate 0.4 Consisting of parts by weight, 1.6 parts by weight of preservative, 5.0 parts by weight of sulfate agent, 10 parts by weight of mineral oil, 5.0 parts by weight of squalane, 5.0 parts by weight of sodium hyaluronate, 5.0 parts by weight of propylene glycol, and 40 parts by weight of purified water Whitening cosmetics containing natural extracts of natural situation mushrooms. According to claim 1, Instead of 20 parts by weight of the natural mushroom extract, 1.6 parts by weight of preservatives, 5.0 parts by weight of a sulfate agent, 40 parts by weight of purified water, 18 parts by weight of natural mushroom extract, 1.6 parts by weight of sulfate agent, 0.2 parts by weight of benzophenone, 0.3 parts by weight of oxybenzone, 0.5 parts by weight of octylmethoxycinnamate, 30 parts by weight of purified water, melanogenesis inhibitory peptide Whitening cosmetics containing a natural mushroom extract, characterized in that the 5.0 parts by weight, licorice extract 5.0 parts by weight, arbutin 1.0 parts by weight, rice extract 5.0 parts by weight. According to claim 1, 20 parts by weight of said natural mushroom extract, 3.0 parts by weight of cetostearyl alcohol, 1.0 parts by weight of beeswax, 3.0 parts by weight of self-emulsifying monostearate monosulfate, 1.0 parts by weight of lysosorbate 60, Instead of 0.4 parts by weight of sorbitan stearate, 1.6 parts by weight of preservative, 5.0 parts by weight of sulfate agent, 10 parts by weight of mineral oil, 5.0 parts by weight of squalane, and 40 parts by weight of purified water, 5.0 parts by weight of natural situation mushroom extract, 5.0 parts by weight of glycerin, 0.05 parts by weight of disodium ethylenediamine tetraacetate, 75 parts by weight of purified water, 3.0 parts by weight of ethanol, 1.25 parts by weight of antioxidant, polyoxyethylene cured caster Whitening cosmetics containing natural situation mushroom extract, characterized in that consisting of 0.7 parts by weight free. According to claim 3, Instead of 75 parts by weight of purified water and 1.25 parts by weight of sulfate agent, 59 parts by weight of purified water, 0.2 parts by weight of oxybenzone, 0.5 parts by weight of octylmethoxy cinnamate, 5.0 parts by weight of melanogenesis inhibitor, 5.0 parts by weight of licorice extract, 1.0 parts by weight of arbutin, 5.0 parts by weight of rice extract And, whitening cosmetics containing natural situation mushroom extract, characterized in that consisting of 0.55 parts by weight of antioxidant.