KR100647306B1 - 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 - Google Patents

아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 Download PDF

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KR100647306B1
KR100647306B1 KR1020040111165A KR20040111165A KR100647306B1 KR 100647306 B1 KR100647306 B1 KR 100647306B1 KR 1020040111165 A KR1020040111165 A KR 1020040111165A KR 20040111165 A KR20040111165 A KR 20040111165A KR 100647306 B1 KR100647306 B1 KR 100647306B1
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dianhydride
group
integer
nucleic acid
formula
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KR1020040111165A
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Korean (ko)
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KR20060072505A (ko
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황규연
김준호
유창은
이헌주
임희균
정성영
심저영
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삼성전자주식회사
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Priority to KR1020040111165A priority Critical patent/KR100647306B1/ko
Priority to DE602005025077T priority patent/DE602005025077D1/de
Priority to EP05027215A priority patent/EP1674570B1/de
Priority to JP2005367060A priority patent/JP4527054B2/ja
Priority to US11/317,949 priority patent/US7402390B2/en
Publication of KR20060072505A publication Critical patent/KR20060072505A/ko
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Publication of KR100647306B1 publication Critical patent/KR100647306B1/ko
Priority to US12/141,894 priority patent/US7695678B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/364—Amphoteric or zwitterionic ion-exchanger
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3285—Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J43/00—Amphoteric ion-exchange, i.e. using ion-exchangers having cationic and anionic groups; Use of material as amphoteric ion-exchangers; Treatment of material for improving their amphoteric ion-exchange properties
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
KR1020040111165A 2004-12-23 2004-12-23 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법 KR100647306B1 (ko)

Priority Applications (6)

Application Number Priority Date Filing Date Title
KR1020040111165A KR100647306B1 (ko) 2004-12-23 2004-12-23 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
DE602005025077T DE602005025077D1 (de) 2004-12-23 2005-12-13 Methode zur Isolation von Nucleinsäure mittels positiv geladenem Material bei einem ersten pH Wert enthaltend Amino- und Carboxylgruppen
EP05027215A EP1674570B1 (de) 2004-12-23 2005-12-13 Methode zur Isolation von Nucleinsäure mittels positiv geladenem Material bei einem ersten pH Wert enthaltend Amino- und Carboxylgruppen
JP2005367060A JP4527054B2 (ja) 2004-12-23 2005-12-20 核酸の分離方法
US11/317,949 US7402390B2 (en) 2004-12-23 2005-12-23 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group
US12/141,894 US7695678B2 (en) 2004-12-23 2008-06-18 Method of isolating nucleic acid using material positively charged at first pH and containing amino group and carboxyl group

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020040111165A KR100647306B1 (ko) 2004-12-23 2004-12-23 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법

Publications (2)

Publication Number Publication Date
KR20060072505A KR20060072505A (ko) 2006-06-28
KR100647306B1 true KR100647306B1 (ko) 2006-11-23

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KR1020040111165A KR100647306B1 (ko) 2004-12-23 2004-12-23 아미노기와 카르복실기를 포함하고 제1 ｐＨ에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법

Country Status (5)

Country Link
US (2) US7402390B2 (de)
EP (1) EP1674570B1 (de)
JP (1) JP4527054B2 (de)
KR (1) KR100647306B1 (de)
DE (1) DE602005025077D1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101015502B1 (ko) 2008-09-09 2011-02-22 삼성전자주식회사 제1 ｐＨ에서 양전하 및 제2 ｐＨ에서 음전하를 띠는 화합물 및 그를 이용하여 핵산을 분리하는 방법

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KR100829574B1 (ko) * 2006-01-03 2008-05-14 삼성전자주식회사 마이크로어레이용 기판, 그 마이크로어레이용 기판을이용하여 생분자를 분석하는 방법, 및 그 마이크로어레이용기판을 포함하는 랩온어칩
US20070190526A1 (en) * 2006-02-16 2007-08-16 Nexgen Diagnostics Llc Methods of extracting nucleic acids
KR100763925B1 (ko) * 2006-09-29 2007-10-05 삼성전자주식회사 탄소나노튜브 및 실리카 비드를 이용한 세포 또는바이러스로부터 핵산의 분리 방법 및 장치
KR101569832B1 (ko) * 2008-11-19 2015-11-18 삼성전자주식회사 시료 중의 게놈 ｄｎａ 및 플라스미드 ｄｎａ를 서로 분리하는 방법 및 그를 위한 키트
DE102008063003A1 (de) * 2008-12-23 2010-06-24 Qiagen Gmbh Nukleinsäureaufreinigungsverfahren
WO2011081869A2 (en) * 2009-12-14 2011-07-07 Betty Wu Method and materials for separating nucleic acid materials
JP5899731B2 (ja) 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
GB201217405D0 (en) * 2012-09-28 2012-11-14 Fermentas Uab Protein removal agent
CN104513819B (zh) * 2014-12-17 2020-05-05 吉林大学 一种选择性提取dna的方法
KR101696549B1 (ko) * 2015-07-31 2017-01-13 전북대학교산학협력단 수소이온농도 대응 검체 스왑 장치
CN108097336B (zh) * 2017-10-25 2019-02-26 重庆博蓝鹰生物技术有限公司 两性解离离子交换介质、应用方法和分离容量标定方法

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US20010018513A1 (en) 1997-12-06 2001-08-30 Baker Matthew John Isolation of nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6562573B2 (en) 1999-01-27 2003-05-13 Folim G. Halaka Materials and methods for the purification of polyelectrolytes, particularly nucleic acids
US20030198968A1 (en) 2002-04-23 2003-10-23 Matson Robert S. Polymer-coated substrates for immobilization of biomolecules and cells

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KR100668337B1 (ko) * 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은ｐＨ 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20010018513A1 (en) 1997-12-06 2001-08-30 Baker Matthew John Isolation of nucleic acids
US6562573B2 (en) 1999-01-27 2003-05-13 Folim G. Halaka Materials and methods for the purification of polyelectrolytes, particularly nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US20030198968A1 (en) 2002-04-23 2003-10-23 Matson Robert S. Polymer-coated substrates for immobilization of biomolecules and cells

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101015502B1 (ko) 2008-09-09 2011-02-22 삼성전자주식회사 제1 ｐＨ에서 양전하 및 제2 ｐＨ에서 음전하를 띠는 화합물 및 그를 이용하여 핵산을 분리하는 방법

Also Published As

Publication number Publication date
EP1674570A3 (de) 2008-08-13
JP2006176521A (ja) 2006-07-06
JP4527054B2 (ja) 2010-08-18
KR20060072505A (ko) 2006-06-28
US7695678B2 (en) 2010-04-13
US20090005265A1 (en) 2009-01-01
EP1674570A2 (de) 2006-06-28
US7402390B2 (en) 2008-07-22
EP1674570B1 (de) 2010-12-01
US20060154284A1 (en) 2006-07-13
DE602005025077D1 (de) 2011-01-13

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