KR100579170B1 - Manufacturing method of cosmetic composition containing carrot extract with ?-carotene - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a beta-carotene-containing carrot extract and a method for preparing the same, wherein the cosmetic composition of the present invention contains beta-carotene extracted with at least one solvent selected from the group consisting of ethanol, acetonitrile and tetrahydrofuran. Carrot extract contains 0.01 to 3.0% by weight based on dry weight. The cosmetic composition according to the present invention not only has excellent moisturizing power and usability, but also has an elastase inhibiting ability to enhance skin elasticity.

Beta-carotene, carrot extract, cosmetics, elastase

Description

Manufacturing method of cosmetic composition containing carrot extract containing beta carotene {Manufacturing method of cosmetic composition containing carrot extract with β-carotene}

The present invention relates to a cosmetic composition comprising a carrot extract containing beta carotene and a method for producing the same.

In the human body, the skin acts as a protective film that protects the living body from the external environment. It prevents the loss of biological components, such as water and electrolytes, and prevents harmful substances from entering the body. . Such skin is largely divided into epidermal layer, dermal layer and subcutaneous fat layer. The epidermal layer is composed of keratinocytes and melanocytes. Since the outermost keratinocyte layers of skin are in direct contact with the external environment, they require strong resistance to physical, chemical, or permeation of substances, and prevent the loss of moisture from the body to the outside. At the same time, it should maintain flexibility by retaining adequate moisture on its own.

In general, the moisture content of the skin is about 70% in the dermal layer, but decreases toward the epidermal layer, which is about 10 to 30% in the keratinocyte layer. The water supplied from the dermal layer is mainly moved up the stratum corneum by passive diffusion and finally discharged to the outside. This is called Trans Epedermal Water Loss (TEWL), and it is known that it is the sebaceous component and the epidermal lipid which are the lipid membrane components of the keratinocyte layer to maintain the appropriate level of transdermal moisture loss. In addition, the keratinocyte layer is known to play an important role in moisturizing the skin due to the presence of a substance having a hydrophilic water retention ability called a natural moisturizing factor (NMF). When the moisture of the normal keratinocyte layer is maintained about 10 to 30%, the skin is smooth and soft, and the body protects normally.

However, when the moisture content of the keratinocyte layer is less than 10%, the skin becomes rough and the body loses its protective function, causing aging. For example, in the case of dry skin, the aggregation force of keratinocytes is weakened, and scaling phenomenon in which keratinocytes are peeled off from the surface of the skin, such as scale fragments, occurs. Thus, the drying of the skin is the moisture content of the keratinocyte layer. This is because it is less than normal skin. In addition, even healthy skin may suffer from a lack of moisture due to harsh external environment, ie, wind, cold weather, sunlight, face wash, shaving, and so on, may cause a bad skin condition.

Therefore, it is very important to properly maintain the moisture content of the keratinocyte layer of the skin. To this end, cosmetics such as sebum-like components, NMF components, or moisturizers such as polyols were added and used. For example, glycerin, solitol, and the like having three or more hydroxyl groups (OH groups) as water-soluble polyols exhibit excellent moisturizing properties, but are highly sticky, making them unpleasant when used, and having two hydroxyl groups (OH groups), 1, 3-butylene glycol may cause side effects of the skin. In addition, other natural moisturizing factors, such as sodium pyrrolidone carboxylate (PCA-Na), sodium lactate, urea, etc., are highly electrolytic and thus impair the emulsion stability of cosmetics, and also moisturize amino acids, collagen and elastin. There is a capacity, but the moisturizing ability was limited.

Therefore, the technical problem to be achieved by the present invention is to solve the above problems, excellent moisture retention ability to improve the dry skin, not only excellent and comfortable to use, but also elastase inhibiting ability to contain beta carotene can improve the elasticity of the skin To provide a cosmetic composition comprising a carrot extract and a method for producing the same.

In order to achieve the above technical problem, the present invention comprises a beta carotene-containing carrot extract extracted with any one or more solvents selected from the group consisting of ethanol, acetonitrile and tetrahydrofuran 0.01 to 3.0% by weight based on dry weight To provide a composition.

In addition, the present invention (a) treating the carrot with any one or more solvent selected from the group consisting of ethanol, acetonitrile and tetrahydrofuran to obtain a carrot extract containing beta carotene; (b) dissolving the carrot extract in water to which 10-50 moles of ethylene oxide is added to add a nonionic surfactant to prepare a stable aqueous solution; And (c) adding 1 to 99% by weight of the aqueous solution based on the total weight of the cosmetic to prepare a cosmetic composition. The method of manufacturing the above-described cosmetic composition comprising the;

Hereinafter, the cosmetic composition according to the present invention will be described in detail.

The cosmetic composition according to the present invention contains beta carotene-containing carrot extract extracted with any one or more solvents selected from the group consisting of ethanol, acetonitrile and tetrahydrofuran based on a dry weight of 0.01 to 3.0% by weight. Extracts obtained by extracting carrots with ethanol, acetonitrile and tetrahydrofuran alone or with a mixed solvent thereof contain various components in addition to a large amount of beta carotene. Beta-carotene is a red crystalline substance with a slight smell and taste. It is a precursor of vitamin A, which is widely distributed in the plant kingdom. Beta-carotene is known to be effective in improving the reproduction of animals as well as the activity of vitamin A, and is effective in inhibiting the production of carcinogens and the destruction of cancer cells and the production of tumor necrosis factor. Unlike vitamin A, beta-carotene is known to have no side effects even in excess dose, and is distributed in the epidermal and dermal layers of the skin to regulate biofunction and to remove oxygen from the skin.

The present inventors have found that when a predetermined amount of the extract obtained by extracting carrots with ethanol, acetonitrile and tetrahydrofuran alone or with a mixed solvent thereof is added to the cosmetics, the moisture retention, the feeling of use and the inhibition of elsastatase are greatly improved. It has been found and completed the present invention.

Looking at the manufacturing method of the cosmetic composition according to the present invention in detail as follows.

3 L of ethanol, acetonitrile and tetrahydrofuran solvent alone or a mixture of them were added to 1 kg of carrot ground powder, boiled for 5 hours in a reflux extractor equipped with a cooling capacitor, extracted at high temperature, and then extracted at a low temperature of about 55 degrees. Minimize the denaturation of the extract. Subsequently, the resultant was filtered with a 300 mesh filter cloth, and then the residue was extracted once more in the same manner, and then filtered and removed from all carrot residues using a filtration device having a size of 3 um, 1 um, and 0.5 um. Thereafter, each resultant was concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser, and then lyophilized to obtain a carrot extract containing beta carotene.

Subsequently, the obtained carrot extract is a nonionic surfactant in which 10 to 50 mol of ethylene oxide is added to water at an appropriate concentration, that is, nonylphenyl ether (EO12), oleyl ether (EO15), and octyldodecyl ether (EO16). ), Sorbitan monolaurate, (EO20), octyldodecyl ether (EO25), hardened castor oil (EO40) using one or two or more non-ionic surfactants that are not easily soluble in water A stable aqueous solution is prepared by stably solubilizing the components, and then the aqueous solution is added 1 to 99% by weight based on the total weight of the cosmetic so that the carrot extract is contained in 0.01 to 3.0% by weight based on the dry weight. According to the present invention, the cosmetic composition containing the carrot extract may be prepared in a formulation of cream, lotion, lotion, essence and the like.

Hereinafter, the present invention will be described in detail with reference to Examples. However, embodiments according to the present invention can be modified in many different forms, the scope of the present invention should not be construed as limited to the embodiments described below. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example of Extracting Carrot Extract Containing Beta Carotene

First, the carrots frozen at a temperature between −10 and −30 ° C. were left at room temperature. Subsequently, 3 L of ethanol solvent was added to 1 kg of carrot pulverized, boiled in a reflux extractor equipped with a cooling capacitor for 5 hours and extracted at high temperature, and then extracted at a low temperature of about 55 degrees to minimize extract denaturation. Subsequently, the resultant was filtered with a 300 mesh filter cloth, and the residue was extracted once more in the same manner, and then filtered and removed all the carrot residues by using a filtration device having a size of 3 um, 1 um, and 0.5 um. Thereafter, each of the results was concentrated under reduced pressure in a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain a carrot extract containing beta carotene.

Subsequently, the obtained carrot extract was dissolved in water to which nonylphenyl ether (EO12) was added to solubilize useful components that are insoluble in water, thereby preparing a stable aqueous solution in which the carrot extract was solubilized 0.5 wt% stably. Was added.

Examples 1 and 2 and Comparative Example 1

1 to 5 in the composition ratio shown in Table 1 After dissolving the water phase parts and the oil phase parts of 6 to 14 to 75-80 ° C. to completely dissolve them, the oil phase parts were slowly added to the water phase parts and emulsified at 75-80 ° C. at 3000 rpm for 5 minutes. During the emulsification, the neutralizing agent of claim 15 was added to neutralize, and then cooled after the completion of the first emulsification. Subsequently, the fragrance and the additive of claim 16 were added at 50 ° C., and then emulsified for 2 minutes at 3000 rpm of a homomixer, cooled, and finished at 30 ° C. to prepare a cream.

Example 3 and Comparative Example 2

By the composition ratio shown in Table 2 below 1 to 5 paragraphs After dissolving the water phase parts and the oil phase parts of 6 to 12 to 75-80 ° C. to completely dissolve them, the oil phase parts were slowly added to the water phase parts and emulsified at 75-80 ° C. for 5 minutes at a homomixer 3000 rpm. During the emulsification, the neutralizing agent of claim 13 was added to neutralize, followed by cooling after completion of the first emulsification, and the fragrance and additive of claim 14 were added at 50 ° C. After emulsifying for 2 minutes at 3000 rpm for a homomixer, the mixture was cooled and terminated at 30 ° C. to prepare a lotion.

Example 4 and Comparative Example 3

By the composition ratio shown in Table 3 below 1 to 4 paragraphs After the aqueous part and the alcohol parts of 5 to 9 were dissolved in a mixer at room temperature, respectively, the solubilized alcohol parts were gradually added to the aqueous part. After all the alcohol parts were added and mixed for 10 to 20 minutes to prepare a lotion.

Example 5 and Comparative Example 4

To the composition ratio shown in Table 4 1 to 5 paragraphs After dissolving the water phase parts and 6-10 oil phase parts to 75-80 ° C. to completely dissolve them, the oil phase parts were slowly added to the water phase parts and emulsified at 75-80 ° C. for 5 minutes at a homomixer 3000 rpm. During the emulsification, the neutralizing agent was added to neutralize the eleventh agent, and after completion of the first emulsification, it was cooled. The resultant was then cooled and terminated at 30 ° C. to produce an essence.

The following experiment was performed about the sample of the Example and the comparative example obtained by the method mentioned above.

Moisture Retention Evaluation

In order to evaluate the moisturizing, the moisture retention test for the Examples and Comparative Examples was carried out as follows. The subjects were 20 subjects, and the skicon-200, a moisture content measuring instrument, was used, and the temperature was maintained at 25 ° C. and 50% relative humidity for accurate experiments. A sample of 0.03 g per 16 cm ^{2 of} skin was applied, and skin electrical conductivity after 2 hours and 4 hours after application was measured, and the difference in measured values before and after application was calculated and shown in Table 5 below. The evaluation values were averaged three times each, and the unit was ㎲.

Transdermal moisture loss measurement

In order to evaluate the moisturizing, the transdermal moisture loss measurement test for the Examples and Comparative Examples was carried out as follows. Twenty subjects were assessed. Tewameter, a moisture loss measuring instrument, was used for evaluation and was maintained at 25 ° C and 50% relative humidity for accurate experiments. The amount applied to the skin was applied to 0.30 g of sample per 16 cm ² every morning and evening, and after 4 weeks, the transdermal moisture loss was measured before and after each application, and the difference is shown in Table 6 below. The evaluation values were averaged after three measurements each, and the unit was mg / cm ^2, and the smaller the value, the better the effect.

Usability Evaluation

Samples of the above Examples and Comparative Examples were divided among 30 subjects and allowed to use continuously for one month, and then sensory evaluation was performed on the feelings of absorbency, applicability, softness, moisturizing power, and the like. The evaluation results were averaged and shown in Table 7 below.

(Sensory Evaluation Criteria)

Very good: 10 points, Average: 5 points, Very bad: 1 point

Antielastase Reviews

In the tissue bath containing the buffer solution of pH 7.4, tendons of width and length were fixed respectively. The dry tissue not only changes with the change in length, but also extends and is threaded into a lever that is converted into an electrical signal and recorded in a potentiometric recorder. After 20 minutes of preconditioning, the cells were replaced with another buffer solution containing leukocyte elastase. As a result, the activity of elastase confirmed that the dry tissue was broken after 132 minutes (min) according to the continuous relaxation of the dry tissue, and the standard of each sample was evaluated by adding the same amount to the buffer solution. It is shown in Table 8 below. The unit is min, and it is judged that the longer the time, the elastase inhibitory ability.

As such, the cosmetic composition containing the beta carotene-containing carrot extract extracted according to the present invention is excellent in moisturizing power and usability, and has an elastase inhibiting ability to enhance skin elasticity. In addition, since carrot extract is a natural substance, cosmetics containing it can also be used safely without side effects on the skin.

Claims (3)

delete delete (a) treating the carrot with at least one solvent selected from the group consisting of ethanol, acetonitrile and tetrahydrofuran to obtain a carrot extract containing beta carotene; (b) dissolving the carrot extract in water to which 10-50 moles of ethylene oxide is added to add a nonionic surfactant to prepare a stable aqueous solution; And (c) adding 1 to 99% by weight of the aqueous solution based on the total weight of the cosmetic to prepare a cosmetic composition; method of producing a cosmetic composition comprising a.