CN110464679B - Mud rubbing device and preparation method thereof - Google Patents

Mud rubbing device and preparation method thereof Download PDF

Info

Publication number
CN110464679B
CN110464679B CN201910843812.5A CN201910843812A CN110464679B CN 110464679 B CN110464679 B CN 110464679B CN 201910843812 A CN201910843812 A CN 201910843812A CN 110464679 B CN110464679 B CN 110464679B
Authority
CN
China
Prior art keywords
mud
mixing
cutin
rubbing device
water
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201910843812.5A
Other languages
Chinese (zh)
Other versions
CN110464679A (en
Inventor
龚德明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangzhou Biotechnology Co ltd
Original Assignee
Guangzhou Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangzhou Biotechnology Co ltd filed Critical Guangzhou Biotechnology Co ltd
Priority to CN201910843812.5A priority Critical patent/CN110464679B/en
Publication of CN110464679A publication Critical patent/CN110464679A/en
Application granted granted Critical
Publication of CN110464679B publication Critical patent/CN110464679B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/41Amines
    • A61K8/416Quaternary ammonium compounds
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706Algae
    • A61K8/9722Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00Cosmetics or similar toiletry preparations
    • A61K8/18Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783Angiosperms [Magnoliophyta]
    • A61K8/9794Liliopsida [monocotyledons]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61QSPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00Preparations for care of the skin
    • A61Q19/10Washing or bathing preparations
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/20Chemical, physico-chemical or functional or structural properties of the composition as a whole
    • A61K2800/28Rubbing or scrubbing compositions; Peeling or abrasive compositions; Containing exfoliants

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Birds (AREA)
  • Epidemiology (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Dermatology (AREA)
  • Cosmetics (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention provides a mud rubbing device and a preparation method thereof. The mud rubbing device comprises the following components in percentage by weight: 0.05-2% of aloe vera leaf extract, 0.05-2% of pinus tomentosa extract, 0.4-1.1% of cutin exfoliating agent, 1.5-3% of thickening agent, 4-21% of humectant and the balance of water. The mud rubbing device is prepared by firstly mixing and homogenizing the thickening agent, the humectant, a part of the cutin peeling agent and water, and then adding the rest of the components and uniformly mixing. The mud rubbing device provided by the invention can quickly remove aged cutin without damaging skin, and has the advantages of quick mud rising, tender and smooth skin, no hair sticking and easiness in washing.

Description

Mud rubbing device and preparation method thereof
Technical Field
The invention belongs to the technical field of washing and caring articles, and particularly relates to a mud rubbing device and a preparation method thereof.
Background
The human skin cells are continuously subjected to cycles of generation, aging and death, the aged and dead cells form cutin, and the newly generated cells gradually move to the cutin. The metabolism of the human skin is influenced by many factors such as diet, work and rest, mood, age, weather, etc. When the diet is unbalanced, the work and rest are irregular, or the skin is aged, the metabolism of skin cells is slowed down, and keratinocytes cannot normally fall off and are accumulated on the surface of the skin, so that the skin is rough and dull. In addition, when air dries, it also causes skin cells to be starved of water, accelerating the aging death and the keratin accumulation. Too thick stratum corneum will further affect the normal metabolism of the skin and exacerbate skin problems.
The mud rubbing device is also called mud rubbing bath device, is a washing and protecting cleaning article for removing aged cutin and dirt of skin, and can be smeared on the skin and gently rubbed to lead the aged cutin and the dirt to be gathered into mud scraps which are removed after being washed by water. CN 104546655A discloses a novel mud rubbing bathrobe, which is prepared from cumin plant mud-lifting factor, CHAMWHA composite essence, seaweed essence, mineral substances, vitamins and essential oil, wherein the cumin plant mud-lifting factor is extracted from the cumin cactus root sac in the Mexico desert. CN 104825345A discloses a mousse formula for removing dead skin, which consists of 30-60% of water agent and 40-70% of propellant; wherein the aqueous solution comprises: 1-10% of glycerol, 3-10% of sodium laureth sulfate, 3-5% of cocamidopropyl betaine, 1-3% of carbomer, 1-3% of behenyl trimethyl ammonium chloride and the balance of water; the product has a lot of fine and dense foams, and can deeply remove stratum corneum necrotic cells and promote skin metabolism.
However, the common mud rubbing device at present has the following disadvantages: 1. the mud is difficult to be generated by repeatedly kneading, and some mud is even not generated, and the generated mud is rare and the use feeling is poor; 2. the kneaded mud easily sticks to body hair, is difficult to wash, and has poor use feeling. Therefore, a mud rubbing product which has the advantages of quick mud lifting, no hair sticking and easy washing is to be developed to meet the market demand.
Disclosure of Invention
Aiming at the defects in the prior art, the invention aims to provide a mud rubbing device and a preparation method thereof. The mud rubbing device can quickly remove aged cutin without damaging skin, and has the advantages of quick mud lifting, tender and smooth skin, no hair sticking and easy washing.
In order to achieve the purpose, the invention adopts the following technical scheme:
in a first aspect, the invention provides a mud rubbing device, which comprises the following components in percentage by weight:
0.05-2% of aloe vera leaf extract, 0.05-2% of pinus tomentosa extract, 0.4-1.1% of cutin exfoliating agent, 1.5-3% of thickening agent, 4-21% of humectant and the balance of water.
The aloe vera leaf extract contains active substance polysaccharide, amino acid, polypeptide, organic acid, vitamin and other water-soluble components, and can obviously enhance the water absorption performance of skin keratinized tissue; the codiales leptocembrae extract mainly comprises L-citrullinyl-L-arginine and galactosyl glycerol, can promote the synthesis of epidermal cell aquaporin-3, thereby enhancing the water circulation and the skin barrier function of the skin, has extremely strong water replenishing and moisturizing effects, can deeply permeate and moisturize from the surface layer, the middle layer and the deep layer of the skin, and naturally exfoliates through the permeation function.
According to the invention, the aloe barbadensis leaf extract and the pinus tomentosa extract are matched with each other, and the cutin stripping agent is combined, so that the removal of aged cutin can be obviously promoted, and the obtained mud rubbing device has the advantages of quick mud rising, no damage to skin, no hair sticking and easiness in washing.
In the present invention, the aloe vera leaf extract may be 0.05 to 2% by weight, for example, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.31%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, or 2%. Preferably 0.8 to 1.5%.
The pinus hirsutus extract may be 0.05 to 2% by weight, for example, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.1%, 1.2%, 1.31%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, or 2%. Preferably 0.8 to 1.5%.
The exfoliating agent may be 0.4 to 1.1% by weight, for example, 0.4%, 0.45%, 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, 1%, 1.05%, or 1.1%.
The thickener may be 1.5 to 3% by weight, for example, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, or 3% and the like.
The humectant may be present in an amount of 4-21% by weight, for example 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 13%, 15%, 16%, 18%, 20%, 21%, etc.
The following is a preferred technical solution of the present invention, but not a limitation to the technical solution provided by the present invention, and the object and advantageous effects of the present invention can be better achieved and achieved by the following preferred technical solution.
As a preferred technical scheme of the invention, the cutin peeling agent is behenyl trimethyl ammonium chloride.
As a preferred technical scheme, the thickening agent is carbomer.
Carbomer is acrylic acid crosslinked resin obtained by crosslinking pentaerythritol and the like with acrylic acid, is a very important rheology regulator, is an excellent gel matrix, has important purposes of thickening, suspending and the like, has simple process and good stability, and is widely applied to emulsion, cream and gel. Carbomers produce highly effective thickening at very low levels (0.25-0.5% of conventional levels) to produce emulsions, creams, gels and transdermal preparations of a wide range of viscosities and varying rheological properties.
As a preferred embodiment of the present invention, the humectant is selected from one or a combination of at least two of propylene glycol, glycerin or erythritol; typical but non-limiting examples of such combinations are: a combination of propylene glycol and glycerol, a combination of propylene glycol and erythritol, a combination of glycerol and erythritol, or a combination of propylene glycol, glycerol and erythritol.
Among them, erythritol has strong moisturizing and hygroscopic properties, and is useful as a moisturizing agent for cosmetics like glycerin, but does not have the greasy and sticky feeling of glycerin.
As a preferable technical scheme of the invention, the sludger also comprises 0.03-0.9% of solubilizer and 0.01-0.3% of aromatic.
Preferably, the solubilizer is hydrogenated castor oil.
Preferably, the hydrogenated castor oil is PEG-40 hydrogenated castor oil.
The fragrance may be a perfume.
Hydrogenated castor oil is a modified castor oil product, is a common high-efficiency solubilizer, and can uniformly disperse oily substances such as essence, essential oil and the like into water to form a stable and transparent solution. According to the difference of the epoxy group, it can be classified into PEG-40 hydrogenated castor oil, PEG-60 hydrogenated castor oil, PEG-80 hydrogenated castor oil, etc.
In a preferred embodiment of the present invention, the sticky candy further comprises 0.5-1% (e.g., 0.5%, 0.55%, 0.6%, 0.65%, 0.7%, 0.75%, 0.8%, 0.85%, 0.9%, 0.95%, or 1%) of a preservative.
Preferably, the preservative is selected from one or a combination of at least two of methyl hydroxybenzoate, phenoxyethanol or ethylhexyl glycerol; typical but non-limiting examples of such combinations are: methyl paraben in combination with phenoxyethanol, methyl paraben in combination with ethylhexylglycerin, phenoxyethanol in combination with ethylhexylglycerin, and the like.
As a preferred technical scheme of the invention, the mud rubbing device comprises the following components in percentage by weight:
0.8-1.5% of aloe vera leaf extract, 0.8-1.5% of pinus tomentosa extract, 0.4-1.1% of behenyl trimethyl ammonium chloride, 1.5-3% of carbomer, 1-10% of propylene glycol, 3-10% of glycerol, 0.1-1% of erythritol, 0.03-0.9% of PEG-40 hydrogenated castor oil, 0.01-0.3% of aromatic, 0.1-0.3% of methyl hydroxybenzoate, 0.35-0.5% of phenoxyethanol, 0.05-0.2% of ethylhexyl glycerol and the balance of water.
In a second aspect, the invention provides a preparation method of the mud rubbing device, which comprises the following steps:
(1) mixing thickener, humectant, 10-20% (such as 10%, 12%, 13%, 15%, 16%, 18% or 20%) cutin exfoliating agent and water, and homogenizing to obtain homogeneous solution;
(2) and (3) mixing the homogenized liquid obtained in the step (1) with the rest components to obtain the mud rubbing device.
As a preferable technical scheme of the invention, the temperature for mixing and homogenizing in the step (1) is 80-85 ℃, for example, 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃ or 85 ℃ and the like; the time is 5-10min, such as 5min, 6min, 7min, 8min, 9min or 10 min.
Preferably, the mixing and homogenizing in the step (1) are carried out in a vacuum emulsifying pot.
Preferably, the temperature of the mixing in the step (2) is 40-45 ℃, for example, 40 ℃, 41 ℃, 42 ℃, 43 ℃, 44 ℃ or 45 ℃ and the like; the time is 5-10min, such as 5min, 6min, 7min, 8min, 9min or 10 min.
As a preferred technical scheme of the invention, the preparation method comprises the following steps:
(1) adding thickener, humectant, 10-20% cutin exfoliation agent and water into vacuum emulsifying pot, mixing and homogenizing at 80-85 deg.C for 5-10min to obtain homogeneous liquid;
(2) and (2) cooling the homogenized liquid obtained in the step (1) to 40-45 ℃, then adding the rest components, stirring and mixing for 5-10min, cooling to below 38 ℃, and discharging to obtain the pug device.
Compared with the prior art, the invention has the following beneficial effects:
the aloe vera leaf extract contains active substance polysaccharide, amino acid, polypeptide, organic acid, vitamin and other water-soluble components, and can obviously enhance the water absorption performance of skin keratinized tissue; the Pinus tomentosa extract mainly comprises L-citrulline-L-arginine and galactosyl glycerol, can promote synthesis of epidermal cell aquaporin-3, thereby enhancing water circulation and skin barrier function of skin, has extremely strong water replenishing and moisturizing effects, can deeply permeate and moisturize from the surface layer, the middle layer and the deep layer of the skin, and naturally removes cutin through the permeation function. According to the invention, the aloe barbadensis leaf extract and the pinus hirsutus extract are matched with each other, so that the synergy is realized, the effects of removing cutin and supplementing water of the aloe barbadensis leaf extract and the pinus hirsutus extract are improved, and the cutin remover is combined, so that the removal of aged cutin can be obviously promoted, and the effects of supplementing water, preserving moisture and tendering skin can be achieved. The obtained mud kneading device can quickly take out mud in 5-10 seconds, and a small amount of mud can be easily kneaded to obtain rich mud scraps; the skin care product is moist, fine and smooth, only old waste cutin is removed, the skin is not damaged, only dirt is cleaned, moisture is not taken away, and the skin becomes tender and smooth after the skin care product is used; the washing machine is free from hair sticking and easy to wash, can be washed after being rubbed with water, does not need external rubbing and washing, does not need secondary cleaning, and is easy and convenient to use.
Detailed Description
The technical solution of the present invention is further illustrated by the following specific examples. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.
Example 1
The embodiment provides a mud rubbing device which comprises the following components in percentage by weight:
Figure BDA0002194537950000061
the preparation method of the mud rubbing device comprises the following steps:
(1) adding thickener, humectant, 1/10 cutin exfoliant and water into vacuum emulsifying pan, mixing and homogenizing at 80 deg.C for 10min to obtain homogenized solution;
(2) and (2) cooling the homogenized liquid obtained in the step (1) to 45 ℃, then adding the rest components, stirring and mixing for 10min, cooling to 38 ℃, and discharging to obtain the mud rubbing device.
Example 2
The embodiment provides a mud rubbing device which comprises the following components in percentage by weight:
Figure BDA0002194537950000071
the preparation method of the mud rubbing device comprises the following steps:
(1) adding thickener, humectant, cutin remover 1/10 and water into vacuum emulsifying pan, mixing and homogenizing at 85 deg.C for 5min to obtain homogeneous solution;
(2) and (2) cooling the homogenized liquid obtained in the step (1) to 40 ℃, then adding the rest components, stirring and mixing for 10min, cooling to 38 ℃, and discharging to obtain the mud rubbing device.
Example 3
The embodiment provides a mud rubbing device, which comprises the following components in percentage by weight:
Figure BDA0002194537950000072
Figure BDA0002194537950000081
the preparation method of the mud rubbing device comprises the following steps:
(1) adding thickener, humectant, cutin remover 1/10 and water into vacuum emulsifying pot, mixing and homogenizing at 83 deg.C for 8min to obtain homogeneous solution;
(2) and (2) cooling the homogenized liquid obtained in the step (1) to 42 ℃, then adding the rest components, stirring and mixing for 8min, cooling to 38 ℃, and discharging to obtain the mud rubbing device.
Example 4
The embodiment provides a mud rubbing device which comprises the following components in percentage by weight:
Figure BDA0002194537950000082
the preparation method of the mud rubbing device comprises the following steps:
(1) adding thickener, humectant, 1/10 cutin exfoliant and water into vacuum emulsifying pan, mixing and homogenizing at 85 deg.C for 10min to obtain homogenized solution;
(2) and (2) cooling the homogenized liquid obtained in the step (1) to 45 ℃, then adding the rest components, stirring and mixing for 10min, cooling to 38 ℃, and discharging to obtain the mud rubbing device.
Example 5
The embodiment provides a mud rubbing device which comprises the following components in percentage by weight:
Figure BDA0002194537950000091
the preparation method of the mud rubbing device comprises the following steps:
(1) adding thickener, humectant, cutin remover 1/10 and water into vacuum emulsifying pot, mixing and homogenizing at 85 deg.C for 10min to obtain homogeneous solution;
(2) and (2) cooling the homogenized liquid obtained in the step (1) to 45 ℃, then adding the rest components, stirring and mixing for 10min, cooling to 38 ℃, and discharging to obtain the mud rubbing device.
Example 6
The embodiment provides a mud rubbing device, which comprises the following components in percentage by weight:
Figure BDA0002194537950000092
Figure BDA0002194537950000101
the preparation method of the mud rubbing device comprises the following steps:
(1) adding thickener, humectant, cutin remover 1/10 and water into vacuum emulsifying pot, mixing and homogenizing at 85 deg.C for 10min to obtain homogeneous solution;
(2) and (2) cooling the homogeneous liquid obtained in the step (1) to 45 ℃, then adding the rest components, stirring and mixing for 10min, cooling to 38 ℃, and discharging to obtain the slub paste.
Example 7
The difference between the mud rubbing agent and the mud rubbing agent in example 6 is that the weight percentage of the aloe barbadensis leaf extract is 0.05%, the weight percentage of the codium tomentosum extract is 1.95%, and other components and preparation methods are the same as those in example 6.
Example 8
The difference between the mud rubbing agent and the mud rubbing agent in example 6 is that the weight percentage of the aloe barbadensis leaf extract is 1.95%, the weight percentage of the codium tomentosum extract is 0.05%, and other components and preparation methods are the same as those in example 6.
Comparative example 1
The difference between the mud rubbing agent and the mud rubbing agent in example 6 is that the mud rubbing agent does not contain the aloe barbadensis leaf extract, the weight percentage of the codium tomentosum extract is 2%, and other components and the preparation method are the same as those in example 6.
Comparative example 2
The difference between the mud rubbing agent and the mud rubbing agent in example 6 is that the aloe barbadensis leaf extract accounts for 2 wt%, the aloe barbadensis leaf extract does not contain the codium tomentosum extract, and other components and preparation methods are the same as those in example 6.
Comparative example 3
The difference between the mud rubbing device and the mud rubbing device in example 6 is that the weight percentage of the cutin peeling agent is 0.3 percent, and other components and the preparation method are the same as those in example 6.
Comparative example 4
The difference between the mud rubbing agent and the mud rubbing agent in example 6 is that the weight percentage of the thickening agent is 1%, and other components and the preparation method are the same as those in example 6.
Performance evaluation:
(1) skin irritation test:
the swab packs provided in examples 1 to 8 were diluted to 10mg/mL with 20mmol/L PBS solution and used as sample solutions. 180 volunteers between the ages of 30-40 were selected as subjects and randomized into 9 groups of 20 individuals each. 0.05mL of the sample solution was taken by syringe and placed in a chamber of a spot test tape (PBS solution as a blank control), and the spot test tape with the sample solution was immediately stuck to the normal skin of the left forearm of the subject from the lower part thereof in the longitudinal direction while gently pressing the chamber one by one to remove air and to distribute the sample uniformly. The test site is marked for easy observation. The patch was applied every 24 hours and then removed for a second half hour and examined according to the CTFA guidelines for a total of 5 patch tests. On day 6 after completion of skin application, additional examination was performed to observe delayed reactions.
The criteria for the skin irritation test are shown in table 1 below:
TABLE 1
Figure BDA0002194537950000111
Figure BDA0002194537950000121
The results of the skin irritation test are shown in table 2 below:
TABLE 2
Figure BDA0002194537950000122
As can be seen from the results in table 2, the skin reactions of the subjects were all negative for the mud rub strips provided in examples 1 to 8, indicating that the mud rub strips provided by the present invention are non-irritating to human skin.
(2) Erythrocyte hemolysis experiment:
preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use.
Hemolysis experiment:
the strips provided in examples 1-8 were selected and diluted to different concentrations with PBS solution, with 5 concentration gradients set for each sample. Taking 10mL of a dilution of a sample to be detected, adding 200 μ L of the erythrocyte suspension (controlling the final concentration of the sample to be 5, 10, 20, 50 and 100mg/mL respectively), using distilled water as a full-blood-soluble control and PBS solution as a negative control, gently mixing, incubating at 37 ℃ for 30min, centrifuging at 2000r/min for 10min, taking the supernatant, and testing the absorbance (A) at 560nm by using a spectrophotometer560) Calculating the hemolysis rate according to the following formula;
Figure BDA0002194537950000131
a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated50)。
Protein denaturation experiments:
diluting the Mianbao provided in examples 1-8 to 10g/L with PBS, taking 10mL of the dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, taking distilled water as a blank control and 1mg/mL of Sodium Dodecyl Sulfate (SDS) solution as a positive control, mixing gently, incubating at 37 ℃ for 30min, centrifuging at 2000r/min for 10min, taking the supernatant, and testing the absorbance A at 540nm and 575nm respectively with a spectrophotometer540And A575Calculating a protein Denaturation Index (DI) according to the following formula;
Figure BDA0002194537950000132
wherein R is1Blank control group a575Blank control group A540,R2Experimental group a575Experimental group A540,R3Positive control group A575Positive control group A540
Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD50DI, erythrocyte hemolysis assay irritation grading criteria are shown in Table 3 below:
TABLE 3
L/D Grading
﹥100 Has no irritation
10﹤L/D≤100 Micro-stimulation property
1﹤L/D≤10 Mild irritation
0.1﹤L/D≤1 Moderate irritation
L/D≤0.1 Severe irritation
The results of the above-described experiments for hemolysis of erythrocytes are shown in table 4 below:
TABLE 4
Test sample HD50(mg/L) DI(%) L/D Evaluation of
Example 1 12725 0.76 ﹥100 Has no irritation
Example 2 4984 1.38 ﹥100 Has no irritation
Example 3 6326 1.15 ﹥100 Has no irritation
Example 4 5357 1.24 ﹥100 Has no irritation
Example 5 5472 1.27 ﹥100 Has no irritation
Example 6 6639 0.93 ﹥100 Has no irritation
Example 7 5785 1.17 ﹥100 Has no irritation
Example 8 5661 1.22 ﹥100 Has no irritation
As can be seen from the results in Table 4, the samples of examples 1-8 all provided clay liners having L/D values greater than 100, and were non-irritating.
(2) And (3) a mud-forming effect test:
120 female volunteers between the ages of 30-40 were selected as subjects, randomized into 12 groups of 10 people each. Test areas of 3cm × 3cm were drawn on the left and right sides of the face of the subject, 0.5mL of the mud pads provided in examples 1 to 8 and comparative examples 1 to 4 were uniformly applied to the test areas, respectively, and the subject gently rubbed the left side of the face with the left hand and gently rubbed the right side of the face with the right hand, and the time to start mud-up was recorded, and the results are shown in the following table 5:
TABLE 5
Figure BDA0002194537950000141
As can be seen from the results in Table 5, the mud rubbings provided in examples 1-8 were faster to be slimed within 5-10s by compounding the aloe barbadensis leaf extract and the codium tomentosum extract with the exfoliating agent. In addition, since the ratio of the aloe vera leaf extract to the codium tomentosum extract in the pugmo provided in example 7 is small, and the ratio of the aloe vera leaf extract to the codium tomentosum extract in the pugmo provided in example 8 is large, the cooperation effect of the aloe vera leaf extract and the codium tomentosum extract is not sufficiently exerted, the mud lifting time of the pugmo provided in examples 7 and 8 is longer than that of example 6.
Comparative example 1 the aloe vera leaf extract was not contained, and comparative example 2 the pinus tomentosa extract was not contained, and the compounding relationship between the aloe vera leaf extract and the pinus tomentosa extract was broken; comparative example 3 the obtained mud rub has a low mud rising speed and a small mud rising amount because the addition amount of the cutin peeling agent is small and the capability of removing aged cutin is insufficient. Comparative example 4 the same is slower in the mud-lifting speed because the thickener is less added, the viscosity of the mud-rolling device is lower, and the aging cutin and dirt aggregation are not facilitated.
(3) Skin moisturizing effect test
Selecting 15 females and 15 males as test objects, drawing test areas with the size of 3cm multiplied by 3cm on the inner sides of the left forearm and the right forearm of the test objects respectively, and testing the moisture content of the skin of the test areas of the test objects by using a skin moisture tester; then 0.5mL of the mud rub strips provided in examples 1-8 and comparative examples 1-4 were uniformly applied to the test area of the left arm of the subject, the test area of the right arm was applied with placebo cream as a blank control, after 30min, the mud rub strips in the test area of the left arm were wiped off, the skin moisture content in the test area was again measured with a skin moisture tester, and the variation value of the skin moisture content was calculated, and the evaluation criteria and the test results of the skin moisturizing effect were respectively shown in the following tables 6 and 7:
TABLE 6
Grade of moisturizing effect Is on the low side Medium grade Is good Is excellent in
2h change in Water content ≤10% 10-20% 20-30% ≥30%
TABLE 7
Figure BDA0002194537950000151
Figure BDA0002194537950000161
As can be seen from the results in table 7, the mud rub provided by the present invention has moderate moisturizing effect, and helps to keep skin tender and smooth by cleaning dirt without moisture. Examples 7 and 8 the skin moisture content was less increased because the ratio of aloe vera leaf extract to pinus tomentosa extract was either smaller or larger, respectively, which is detrimental to the combination of moisturizing effect of both. Comparative examples 1 and 2 have a low moisturizing effect because only one of the extracts exerts a moisturizing effect without the aloe vera leaf extract and the pinus tomentosa extract, respectively, and the compounding relationship between the aloe vera leaf extract and the pinus tomentosa extract is broken.
The applicant declares that the above description is only a specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and it should be understood by those skilled in the art that any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are within the scope and disclosure of the present invention.

Claims (6)

1. The mud rubbing device is characterized by comprising the following components in percentage by weight:
0.8-1.5% of aloe barbadensis leaf extract, 0.8-1.5% of pinus hirsutus extract, 0.4-1.1% of behenyl trimethyl ammonium chloride, 1.5-3% of carbomer, 1-10% of propylene glycol, 3-10% of glycerol, 0.1-1% of erythritol, 0.03-0.9% of PEG-40 hydrogenated castor oil, 0.01-0.3% of aromatic, 0.1-0.3% of methylparaben, 0.35-0.5% of phenoxyethanol, 0.05-0.2% of ethylhexyl glycerol and the balance of water;
wherein, the behenyl trimethyl ammonium chloride is a cutin peeling agent, the carbomer is a thickening agent, and the propylene glycol, the glycerol and the erythritol are humectants.
2. The mud scraper preparation method according to claim 1, wherein the preparation method comprises the following steps:
(1) mixing and homogenizing a thickening agent, a humectant, 10-20% of a cutin peeling agent and water to obtain a homogenized liquid;
(2) and (2) mixing the homogenized liquid obtained in the step (1) with the rest components to obtain the sludger.
3. The method according to claim 2, wherein the temperature for mixing and homogenizing in step (1) is 80-85 ℃ and the time is 5-10 min.
4. The method according to claim 2, wherein the mixing and homogenizing in the step (1) are performed in a vacuum emulsifying kettle.
5. The method according to claim 2, wherein the mixing in the step (2) is carried out at a temperature of 40 to 45 ℃ for 5 to 10 min.
6. The method of claim 2, comprising the steps of:
(1) adding thickener, humectant, 10-20% cutin exfoliation agent and water into vacuum emulsifying pot, mixing and homogenizing at 80-85 deg.C for 5-10min to obtain homogeneous liquid;
(2) and (2) cooling the homogenized liquid obtained in the step (1) to 40-45 ℃, then adding the rest components, stirring and mixing for 5-10min, cooling to below 38 ℃, and discharging to obtain the pug device.
CN201910843812.5A 2019-09-06 2019-09-06 Mud rubbing device and preparation method thereof Active CN110464679B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201910843812.5A CN110464679B (en) 2019-09-06 2019-09-06 Mud rubbing device and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201910843812.5A CN110464679B (en) 2019-09-06 2019-09-06 Mud rubbing device and preparation method thereof

Publications (2)

Publication Number Publication Date
CN110464679A CN110464679A (en) 2019-11-19
CN110464679B true CN110464679B (en) 2022-06-14

Family

ID=68515127

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201910843812.5A Active CN110464679B (en) 2019-09-06 2019-09-06 Mud rubbing device and preparation method thereof

Country Status (1)

Country Link
CN (1) CN110464679B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111067836B (en) * 2020-01-11 2022-07-26 广州品爵生物科技有限公司 Exfoliating gel and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104523490A (en) * 2014-12-11 2015-04-22 广州栋方日化有限公司 Exfoliate composition with whitening and moisturizing effects and preparation method of exfoliate composition
CN104546655A (en) * 2013-10-17 2015-04-29 李文金 Novel exfoliating body wash
CN104825345A (en) * 2015-05-04 2015-08-12 上海彤颜实业有限公司 Formula of mousse for removing dead skin and production technology thereof
CN106265471A (en) * 2016-08-22 2017-01-04 台山美环健芦荟制品有限公司 A kind of aloetic replenishing water and preserving moisture cosmetic composition and application thereof
WO2019006217A1 (en) * 2017-06-28 2019-01-03 Collidion, Inc. Compositions, methods and uses for cleaning, disinfecting and/or sterilizing

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104546655A (en) * 2013-10-17 2015-04-29 李文金 Novel exfoliating body wash
CN104523490A (en) * 2014-12-11 2015-04-22 广州栋方日化有限公司 Exfoliate composition with whitening and moisturizing effects and preparation method of exfoliate composition
CN104825345A (en) * 2015-05-04 2015-08-12 上海彤颜实业有限公司 Formula of mousse for removing dead skin and production technology thereof
CN106265471A (en) * 2016-08-22 2017-01-04 台山美环健芦荟制品有限公司 A kind of aloetic replenishing water and preserving moisture cosmetic composition and application thereof
WO2019006217A1 (en) * 2017-06-28 2019-01-03 Collidion, Inc. Compositions, methods and uses for cleaning, disinfecting and/or sterilizing

Also Published As

Publication number Publication date
CN110464679A (en) 2019-11-19

Similar Documents

Publication Publication Date Title
EP1561457B1 (en) Cosmetic compositions with long lasting skin moisturizing properties
JPS58213705A (en) Skin conditioning composition
CN110302101B (en) Moisturizing repair emulsion and preparation method thereof
JP2007291125A (en) Cosmetic composition with skin-tightening effect
CN110433113A (en) A kind of essence dew and preparation method containing the anti-ageing composition of moisturizing
CN109431843B (en) Moisturizing repair emulsion and preparation method thereof
US20070253988A1 (en) Methods for peeling skin
CN110464679B (en) Mud rubbing device and preparation method thereof
CN117398326B (en) Skin moisturizing and repairing composition and application thereof
CN113384497B (en) Composition and cosmetic for double moisturizing, repairing and improving skin elasticity
JP2006348000A (en) External preparation for skin
CN115154347B (en) Cleansing cream and preparation method thereof
CN115607479A (en) Moisturizing composition and moisturizing liquid for skin
JP5291365B2 (en) Keratin condition improving agent
JPH03232811A (en) Cosmetic
JP2001288068A (en) Skin cosmetic
KR20110013000A (en) Cosmetic composition for moisturizing skin which alleviates itchiness and dryness by removing keratin and increasing moisture
JP3479048B2 (en) Cosmetics and non-woven fabric impregnated cosmetics
WO2003070180A2 (en) Compositions and methods for delivery of skin cosmeceuticals
JP2004075635A (en) Moisturizing agent and external preparation for skin
KR20170001201A (en) A cosmetic composition containing carrot leaf extract
KR20160037365A (en) Cosmetic compositions for skin moisturizing containing elderflower extract
JP2017507974A (en) Shellac-based skin care lotion
JP6017241B2 (en) Toilet lotion
CN117482028B (en) Long-acting moisturizing emulsion and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right

Effective date of registration: 20220509

Address after: No.5, area 1, community 8, Xinke village, JUNHE street, Baiyun District, Guangzhou City, Guangdong Province 510000

Applicant after: Guangzhou Biotechnology Co.,Ltd.

Address before: Room 301, Hongshi business building, No.11 Kehua Road, Guangzhou private science and Technology Park, no.1633 Beitai Road, Baiyun District, Guangzhou, Guangdong 510000

Applicant before: Guangzhou Liujin scientific research Co.,Ltd.

TA01 Transfer of patent application right
GR01 Patent grant
GR01 Patent grant