KR100572426B1 - 단백질 제조방법 - Google Patents

단백질 제조방법 Download PDF

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Publication number
KR100572426B1
KR100572426B1 KR1019980041127A KR19980041127A KR100572426B1 KR 100572426 B1 KR100572426 B1 KR 100572426B1 KR 1019980041127 A KR1019980041127 A KR 1019980041127A KR 19980041127 A KR19980041127 A KR 19980041127A KR 100572426 B1 KR100572426 B1 KR 100572426B1
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KR
South Korea
Prior art keywords
carbon source
sugar
glucose
feed
promoter
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KR1019980041127A
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English (en)
Korean (ko)
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KR19990036734A (ko
Inventor
카르스텐 헬무쓰
울리바리 루알 로페즈
안네 프랑소와즈 마이어
하인리히 빈프리트 쉴리커
룬 아돌푸스 반
Original Assignee
디에스엠 아이피 어셋츠 비.브이.
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Publication of KR19990036734A publication Critical patent/KR19990036734A/ko
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Publication of KR100572426B1 publication Critical patent/KR100572426B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1019980041127A 1997-10-01 1998-09-30 단백질 제조방법 KR100572426B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97117021.2 1997-10-01
EP97117021 1997-10-01

Publications (2)

Publication Number Publication Date
KR19990036734A KR19990036734A (ko) 1999-05-25
KR100572426B1 true KR100572426B1 (ko) 2006-06-21

Family

ID=8227420

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019980041127A KR100572426B1 (ko) 1997-10-01 1998-09-30 단백질 제조방법

Country Status (12)

Country Link
US (1) US6204012B1 (US06559137-20030506-C00039.png)
EP (2) EP1659173A1 (US06559137-20030506-C00039.png)
JP (1) JP4162774B2 (US06559137-20030506-C00039.png)
KR (1) KR100572426B1 (US06559137-20030506-C00039.png)
CN (1) CN1215175C (US06559137-20030506-C00039.png)
AU (1) AU748187B2 (US06559137-20030506-C00039.png)
BR (1) BR9803807B1 (US06559137-20030506-C00039.png)
CA (1) CA2249061C (US06559137-20030506-C00039.png)
DE (1) DE69834533T2 (US06559137-20030506-C00039.png)
DK (1) DK0911416T3 (US06559137-20030506-C00039.png)
ES (1) ES2264181T3 (US06559137-20030506-C00039.png)
IN (1) IN188151B (US06559137-20030506-C00039.png)

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US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
US6511699B1 (en) * 1999-03-31 2003-01-28 Cornell Research Foundation, Inc. Enzymes with improved phytase activity
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
ES2597503T3 (es) * 2001-10-31 2017-01-19 Huvepharma Eood Alimento para animales que contiene fitasa y método
KR100429331B1 (ko) * 2002-01-29 2004-04-29 재단법인 목암생명공학연구소 재조합 효모를 이용한 에스에이치엘케이엔-1 단백질의대량생산방법
JP4510938B2 (ja) * 2002-07-15 2010-07-28 イミュネックス・コーポレーション 哺乳動物細胞により産生されるタンパク質のシアル酸付加を調節する方法および培地
WO2004024885A2 (en) 2002-09-13 2004-03-25 Cornell Research Foundation, Inc. Using mutations to improve aspergillus phytases
EP2287326A1 (en) 2003-08-08 2011-02-23 Arriva Pharmaceuticals, Inc. Methods of protein production in yeast
SI1684719T1 (sl) * 2003-11-14 2012-07-31 Baxter Int Sestavki alfa antitripsina in postopki zdravljenja ob uporabi takih sestavkov
WO2006036678A2 (en) * 2004-09-22 2006-04-06 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
US20070105768A1 (en) * 2004-11-10 2007-05-10 Rajiv Nayar Dry recombinant human alpha 1-antitrypsin formulation
WO2006100292A1 (en) * 2005-03-24 2006-09-28 Dsm Ip Assets B.V. Process for microbial production of a valuable compound
US7919297B2 (en) * 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
AU2007244753B2 (en) * 2006-04-28 2012-05-17 C5-6 Technologies, Inc. Thermostable cellulase and methods of use
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2106447B1 (en) 2007-01-26 2013-04-17 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
WO2009137574A2 (en) * 2008-05-06 2009-11-12 Archer Daniels Midland Company Development of strains of the thermotolerant yeast hansenula polymorpha capable of alcoholic fermentation of starch and xylan by expression of starch and xylan degrading enzymes
GB201308828D0 (en) 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
CN103159531A (zh) * 2013-04-17 2013-06-19 广西新方向化学工业有限公司 一种含多种植物醇和中微量元素螯合的液体肥料及其制备方法
EP3027762A4 (en) 2013-07-25 2017-06-28 BASF Enzymes LLC Phytase
WO2016077457A1 (en) 2014-11-11 2016-05-19 Clara Foods Co. Methods and compositions for egg white protein production
CN104928271A (zh) * 2015-07-06 2015-09-23 唐亚军 一种木聚糖酶的制备方法
CN105200101B (zh) * 2015-10-19 2019-04-16 华中农业大学 一种毕赤酵母菌体重复发酵表达外源蛋白的方法
CA3146649A1 (en) 2019-07-11 2021-01-14 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244920A1 (en) * 1986-06-05 1987-11-11 Unilever N.V. Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0533492A2 (en) * 1991-09-18 1993-03-24 Amgen Inc. A hepatitis B vaccine formulation incorporating a bile acid salt
KR970700775A (ko) * 1994-10-25 1997-02-12 가와노 다케히코 단백질 제조방법(Method for Producing Protein)
KR0161148B1 (ko) * 1995-10-11 1998-11-16 김은영 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
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EP0423890A3 (en) 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
EP0242007B1 (en) * 1986-11-24 1990-11-07 Unilever N.V. Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof
EP0299108B1 (en) * 1987-07-17 1994-05-18 Rhein Biotech Gesellschaft für biotechnologische Prozesse und Produkte mbH DNA-molecules coding for FMDH control regions and structured gene for a protein having FMDH-activity and their uses
US5667986A (en) * 1989-10-18 1997-09-16 Delta Biotechnology Limited Yeast promoter for expressing heterologous polypeptides
ES2268687T3 (es) 1994-04-25 2007-03-16 Dsm Ip Assets B.V. Polipeptidos con actividad fitasa.
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244920A1 (en) * 1986-06-05 1987-11-11 Unilever N.V. Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0533492A2 (en) * 1991-09-18 1993-03-24 Amgen Inc. A hepatitis B vaccine formulation incorporating a bile acid salt
KR970700775A (ko) * 1994-10-25 1997-02-12 가와노 다케히코 단백질 제조방법(Method for Producing Protein)
KR100405944B1 (ko) * 1994-10-25 2004-03-22 미쯔비시 웰 파마 가부시키가이샤 단백질 제조방법
KR0161148B1 (ko) * 1995-10-11 1998-11-16 김은영 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법

Also Published As

Publication number Publication date
AU8715798A (en) 1999-04-22
CA2249061A1 (en) 1999-04-01
EP0911416B1 (en) 2006-05-17
EP0911416A3 (en) 2000-01-19
DE69834533D1 (de) 2006-06-22
CN1215175C (zh) 2005-08-17
BR9803807A (pt) 2000-03-28
DE69834533T2 (de) 2006-10-12
BR9803807B1 (pt) 2010-11-03
CN1218112A (zh) 1999-06-02
EP0911416A2 (en) 1999-04-28
US6204012B1 (en) 2001-03-20
DK0911416T3 (da) 2006-08-14
CA2249061C (en) 2009-02-24
JPH11169173A (ja) 1999-06-29
AU748187B2 (en) 2002-05-30
IN188151B (US06559137-20030506-C00039.png) 2002-08-24
ES2264181T3 (es) 2006-12-16
KR19990036734A (ko) 1999-05-25
EP1659173A1 (en) 2006-05-24
JP4162774B2 (ja) 2008-10-08

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