KR100520645B1 - Cosmetic composition having anti-lifting effector - Google Patents

Abstract

The present invention relates to a cosmetic composition that improves the elasticity in the dermal layer to improve skin elasticity and form a protein film on the skin surface to pull sagging skin to improve skin sagging, and the cosmetic composition of the present invention is applied to a conventional cosmetic composition. 0.1 to 2% by weight of Decarboxy Carnosine 2HCl (Carcinine), 0.001 to 0.5% by weight of essential oils extracted from cellar, and 0.01 to 5.0% by weight of polymer of wheat protein and polyvinylpyrrolidone (PVP) It is characterized by containing.

Skin sag improvement cosmetic composition according to the present invention is configured as described above is essential oil and moisture retention effect and skin tightening effect of dicarboxy carnosine hydrochloride and elastin degradation inhibitory effect collagen synthesis and By providing a polymer of polyvinylpyrrolidone (PVP) as a certain component in various cosmetics to provide a cosmetic composition having a useful action to improve the skin elasticity of the dermis and improve sagging skin.

Description

Cosmetic composition for improving sagging skin {Cosmetic composition having anti-lifting effector}

The present invention relates to a cosmetic composition that improves skin sagging by pulling sagging skin, and more particularly, improves elasticity of the dermal layer to improve skin elasticity and forms a protein film on the skin surface to draw sagging skin. It relates to a cosmetic composition to improve.

The dermis is a connective tissue containing epidermal appendages originating from nerves, blood vessels, and epidermis. The connective tissue of the dermis is composed of collagen fibers, elastic fibers, and a ground substance with no particular shape, all of which are made by fibroblasts.

Collagen collagen is the main component of the dermis and accounts for 75% of the dry weight of the skin. Collagen molecules are characterized by cross-linking of molecules rather than end-to-molecules, making collagen with tensile strength along with the amino acid structure.

Elastic fibers make up 3% of the dry weight of the dermis and the main function is to provide elasticity to the skin so that the deformed skin returns to its original appearance by the applied force. Mature elastic fibers comprise about 90% of elastin, an amorphous insoluble protein, with the remainder composed of microfiber proteins. Elastin, on the other hand, is broken down by elastase, which reduces skin elasticity.

As a technology for preventing skin sagging by maintaining skin elasticity by solving the problem of skin elasticity reduction, Korean Patent Application No. 2000-0014487 discloses a technique for reducing wrinkles by a tightening effect of a silicone polymer, and Korea Patent application No. 1999-0030187 discloses a technique for enhancing skin elasticity effect by inhibiting elastin degradation and collagen degradation by soybean, malt, and wheat proteins, and Korean Patent Application No. 2001-0043836 discloses a conjugated Tat peptide. Retinol derivatives enhance skin absorption and disclose collagen synthesis and suppress elastin degradation, and Korean Patent Application No. 2000-0013995 discloses a technique for enhancing skin elasticity using Yulpi extract.

The present invention is to provide a cosmetic that can improve the skin sagging, the present inventors have completed the present invention by studying a material that can improve the skin sagging by pulling sagging skin.

That is, an object of the present invention is to provide a cosmetic composition that can improve skin elasticity by improving the elasticity in the dermal layer and improve the skin sagging by forming a protein film on the skin surface to pull sagging skin.

The object of the present invention as described above is not only to improve the skin elasticity by inhibiting the degradation of the elastic fiber elastin and the collagen synthesis enhancement effect, but also to form a polymer protein membrane on the skin surface to give moisture and tightening effect to the skin It was achieved by providing a cosmetic composition obtained by combining a substance having an effective effect in improving the deflection phenomenon of the conventional cosmetic composition with a certain ratio.

Skin sagging cosmetic composition of the present invention for achieving the above object;

0.1 to 2% by weight of Decarboxy Carnosine 2HCl (Carcinine), 0.001 to 0.5% by weight of essential oil extracted from the fruit room, and a polymer of wheat protein and polyvinylpyrrolidone (PVP) in a conventional cosmetic composition It is characterized by containing 0.01 to 5.0% by weight.

Dicarboxy carnosine 2HCl (Carcinine) is a component of the composition according to the composition of the present invention is contained 0.1 to 2% by weight, the dicarboxy carnosine hydrochloride is stabilized by decarboxylation of carnosine It is a substance known to have various effects such as antioxidant effect, inflammatory disease treatment, photoprotective effect. In the present invention, the dicarboxy carnosine hydrochloride has a useful effect that can be applied to increase skin elasticity by showing an collagen synthesis increase effect.

The essential oil extracted from the basement which is another component of the composition according to the composition of the present invention contains 0.001 to 0.5% by weight. Poncirus trifoliata Rafin. When the fruit is less cooked, it is cut into two or three pieces and dried. It is called 'pile' and it is used to treat eczema and the circulation of chi. In the present invention, the substance has a useful effect that can be applied to inhibit elastin degradation and ultimately improve skin elasticity.

Other components of the composition according to the composition of the present invention, the polymer of wheat protein and polyvinylpyrrolidone (polyvinylpyrrolidone, PVP) is contained 0.01 to 5.0% by weight. The polymer has a molecular weight of about 40,000 and its constituent wheat protein and polyvinylpyrrolidone are both used as film forming agents. In the present invention, the polymer has a useful effect that can be applied to reduce the immediate extensibility of the skin and give a noticeable pulling effect.

The present invention constituted as described above includes dicarboxy carnosine hydrochloride, essential oil of fibril, hydrolyzed wheat protein and polyvinylpyrrolidone polymer in a proportion to improve elasticity in the dermal layer and improve skin elasticity. It provides a cosmetic composition having the effect of improving the sagging skin by forming a protein film on the skin surface, hereinafter referred to as the component of the present invention is called as an anti-lifting effect (Anti-lifting effector).

According to another configuration of the present invention, the skin sagging improvement component is applied to the cosmetic for each formulation. More specifically, 0.1% to 2% by weight of decarboxy Carnosine 2HCl (Carcinine) and 0.001% to 0.5% by weight of essential oil extracted from the fruit room and wheat protein and polyvinylpyrrolidone (PVP) It can be prepared in various forms of cosmetics having a skin sagging effect within the range containing 0.01 to 5.0% by weight of the polymer.

Hereinafter, the present invention will be described in more detail through Examples, Comparative Examples, and Experimental Examples. However, these Examples, Comparative Examples, and Experimental Examples are provided only to assist in understanding the present invention, and the scope of the present invention is not limited to the following Examples and Experimental Examples.

Example 1-2, Comparative Example 1-2

According to the configuration of the present invention to prepare a cosmetic composition of the following Table 1.

ingredient Content (% by weight) Example 1 Example 2 Comparative Example 1 Comparative Example 2 Carbomer 0.5 - - - Hydrolyzed Wheat Protein (250,00 Mw) - - 5 - Hydrolyzed Wheat Protein (500,000 Mw) - - - One Hydrolyzed Wheat Protein and Polyvinylpyrrolidone Polymers One One - - Purified water Quantity Quantity Quantity Quantity Triethanolamine Correct amount (pH 6.5 ~ 7.0) - - - Sum 100 100 100 100

Example 3, Comparative Example 3-6

Next, a cosmetic composition was prepared as shown in Table 2.

ingredient Example 3 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 glycerin 5.0 5.0 5.0 5.0 5.0 1,3-butylene glycol 5.0 5.0 5.0 5.0 5.0 Sesquioleate sorbitan 0.4 0.4 0.4 0.4 0.4 Polylauric acid polyoxyethylene sorbitan 1.0 1.0 1.0 1.0 1.0 Paraoxybenzoic Acid Methyl 0.2 0.2 0.2 0.2 0.2 ethanol 2.0 2.0 2.0 2.0 2.0 Vaseline 5.0 5.0 5.0 5.0 5.0 Cetanol 0.2 0.2 0.2 0.2 0.2 Dicarboxy Carnosine Hydrochloride 0.1 - 0.1 0.1 Basement Essential Oil 0.001 - 0.001 - 0.001 Hydrolyzed Wheat Protein and Polyvinylpyrrolidone Polymers One - One One - Purified water Balance Balance Balance Balance Balance

Experimental Example 1

Collagen Fiber Biosynthesis Effect (Effect of Carcinin)

Fibroblasts (fibroblast W138) were incubated for 24 hours with 5 × 10 ⁴ cells / well in a 48 well plate. After removal of the supernatant, dicarboxylic carnosine hydrochloride was treated by diluting the serum-free culture medium by concentration. After 24 hours, the supernatant of each well was collected and the amount of procollagen type IC-peptide (PICP) was measured using a collagen fiber biosynthesis kit (Procollagen Type IC-peptide EIA kit: Takara MK101). Absorbance was measured at 450 nm. TGF-β was used as a positive control and treated at 10 ng / ml.

Dicarboxy carnosine hydrochloride (ug / ml) O.D. Collagen (ng / mL) % of control 0 1.458 52.3 0 5 2.212 79.7 ** 52.7 10 2.357 84.6 ** 61.7 50 2.687 116.6 ** 122.9

As shown in Table 3, dicarboxy carnosine hydrochloride increased collagen synthesis in a concentration-dependent manner. The amount of collagen synthesis at 50 ug / ml of TGF-β used as a positive control was 118.9 ng / ml. In comparison, dicarboxy carnosine hydrochloride was found to have a significant collagen synthesis effect of about 98% at 50 ug / ml.

Experimental Example 2

Elastin Degradation Inhibitory Effect (Effect of Lost Essence)

Into a 96-well microplate, add 100 ul of buffer and 50 ul of fissure essential oil for each concentration. Subsequently, 25ul of nitroanilide (Succinyl-Ala-Ala-Pro-Phe-p-nitroanilide, Calbiochem), which is a substrate, is added thereto, and a reaction is caused by adding 25 ul of the enzyme delastase (Elastase, Sigma) to each well. 20 minutes after the start of the reaction, the absorbance at 410 nm was measured to show the effect of inhibiting the elastin degradation of the silyl essential oil as a ratio to the control group. The results are shown in Table 4.

Concentration (ppm) Elastin inhibitory effect (%) 10 15 50 25 100 50

Experimental Example 3

Tightening effect of skin (effect of wheat protein and polyvinylpyrrolidone polymer)

After two hours of applying the cosmetics prepared according to Examples 2 and Comparative Examples 1 and 2, the upper three upper arms of the upper arm, 10 minutes for 15 seconds with a DTM (Dermal Torque Meter, Dia-Stron Limited, Hampshire, England) Milli Newton meters (mNm) apply. Then remove the DTM device and observe the skin relax for 15 seconds. Immediate extensibility (Ue) measured at this time is used to measure the tightness of the skin formed by the polymer protein. 5 to 6 subjects were carried out for 5 days and the results are shown in Table 5. The change of Ue value is as shown in FIG.

% Change in Ue over 5 days Comparative Example 1 -3.8 ± 0.005 Comparative Example 2 -6.4 ± 0.05 Example 2 8.2 ± 0.005

 As shown in Table 5, it can be seen that the Ue value was significantly decreased during the experiment. Example 2 showed an excellent reduction effect compared to Comparative Examples 1 and 2 at-8.2.

Based on this, the effect of the wheat protein polymer in the gel formulation was determined using the composition of Example 1, and the average Ue change rate over 5 days was -3.0%, and the measured value is shown in FIG. 2.

 Experimental Example 4

Moisture Retention Measurement

This experiment was carried out using conventional essence formulations prepared according to Example 3 and Comparative Example 3-6.

Allow 20 adult men and women to rest in a constant temperature and humidity room for at least 30 minutes, set a 4 × 4 cm 2 compartment inside the lower arm, and apply the product at a thickness of 2 µg / cm 3. Skin moisture content was measured using a coronometer (Corneometer CM 820, Courage + Khazaka electronic Gmbh, Germany). The results are shown in Table 6.

Average value measured before application Average value measured after application 1 hours 2 hours 3 hours 4 hours Example 3 66 85 81 79 71 Comparative Example 3 64 87 82 71 62 Comparative Example 4 62 80 78 73 65 Comparative Example 5 66 86 83 78 69 Comparative Example 6 65 85 79 72 66

As shown in Table 6, the case of Example 3 containing the composition showed an excellent moisture retention capacity compared to Comparative Examples 3, 4, 5, 6.

Experimental Example 5

Usability Test

In Example 3 and Comparative Example 3, 30 female panelists 20 to 35 years old were evaluated by a post-use questionnaire for 3 weeks. The product was used twice daily in the morning and evening and evaluated on a 5-point scale and the results are shown in Table 7.

Test Items Example 3 Comparative Example 3 Tightening 5 12 6 4 10 5 3 4 14 2 3 3 One One 2 Average 3.96 3.3 Moisturizing 5 5 2 4 12 11 3 10 10 2 3 6 One - One Average 3.63 3.23 pepper 5 - - 4 One 2 3 3 One 2 6 8 One 22 19 Average 1.56 1.53

* 5 point scale:

5: Very good (strong stimulus) 4: Good (stimulus) 3: Normal (weak stimulus)

2: bad (normal) 1: very bad (no irritation)

As shown in Table 7, in the case of Example 3 including the composition had a better effect on tightening and moisturizing compared to Comparative Example 3 not included and the irritation was less than normal for both products.

Skin sag improvement cosmetic composition according to the composition of the present invention is essential oil and moisture retention effect and skin tightening effect of dicarboxy carnosine hydrochloride and elastin degradation inhibitory effect collagen synthesis and polyvinyl It contains a polymer of pyrrolidone (polyvinylpyrrolidone, PVP) in a variety of cosmetics to provide a cosmetic composition having a useful action to improve the skin elasticity of the dermis and improve sagging skin.

1 is a graph showing a change in the skin extensibility immediately after applying a sample of the present invention,

Figure 2 is a graph showing the effect of the wheat protein polymer in the gel formulation using the composition of Example 1 as an average value of the change rate of the immediate skin elongation for 5 days.

Claims (2)

0.1 to 2% by weight of Decarboxy Carnosine 2HCl (Carcinine), 0.001 to 0.5% by weight of essential oil extracted from fruit room, and polymer of wheat protein and polyvinylpyrrolidone (PVP) in a conventional cosmetic composition A skin sagging cosmetic composition comprising 0.01 to 5.0% by weight. The cosmetic composition of claim 1, wherein the formulation of the cosmetic composition according to claim 1 is selected from a flexible cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, gel type cosmetic formulation.