KR100493434B1 - The making method of mushroom spawn seed and the application by germination of uncleaned rice and all kinds of a bean include of “Jangdancong”. - Google Patents

Abstract

The present invention utilizes germinated brown rice or germinated soybeans as a medium for mushrooms instead of sawdust used during artificial cultivation, and a method of producing mushroom fruiting bodies and producing brown rice and soybean cultured with mushroom mycelium.

Description

 The making method of mushroom spawn seed and the application by germination of uncleaned rice and all kinds of a bean include of “Jangdancong”.}

The purpose of the present invention is to harvest the mushroom fruiting body using brown rice or soybean as a mushroom medium and the remaining medium was difficult to recycle previously, but the remaining medium is also a high-performance mushroom mycelium germinated brown rice and germinated soybeans for use as a very beneficial material for health For that purpose.

Conventional cultivation of mushrooms were all using wood or sawdust. It is true that wood and sawdust took considerable time to decompose and be used as a nutritional source for mushroom mycelia. The present invention, unlike the conventional method, by inoculating the mycelia of liquid culture by germinating brown rice or soybean as a medium raw material to harvest the mushroom fruiting body in a shorter period than the conventional method, and waste wood and waste sawdust medium It relates to a method of producing a high-quality germinated brown rice and germinated soybean mushroom hyphae faithfully absorbed.

Conventional mushroom artificial cultivation is used to mix the rice bran, ganggang, dried sage, corn cob, etc. with the sawdust as the main raw material, the present invention is to use germinated brown rice or germinated soybean as a mushroom medium.

Brown rice is stripped only chaff, the outer shell is very hard. This is because phytic acid is present. In other words, the brown rice leaves the rice bran layer or seeds without cutting.

Brown rice contains about 4 times more vegetable fiber and vitamin B1 than rice, and about 2 times more fat, phosphorus and iron. On the other hand, a significant increase in nutrients was observed during soybean germination.

In the present invention, only brown rice and soybeans are germinated, not just brown rice or soybean as a raw material. Germinated brown rice and soybeans are sprouted up to 1-10mm by supplying proper moisture, temperature, and oxygen to brown rice and soybeans to maximize the nutrition and function of brown rice and soybeans. When sprouted, phytic acid is changed to phosphorus and itositol, making it easier to break down, and when germinated, new substances such as vitamins, amino acids, enzymes, SOD, arabinoxyran and gamma-orizanol are produced. In other words, germinating grains, including soybeans, creates new ingredients that have never been seen before.The germinated brown rice, in particular, has germinated vitamin B1, B2, sugar, fat, minerals, and dietary fiber. The nutrients are multiplied several times so that the mushroom mycelium is easily broken down and enough nutrition is used to fill the fruiting bodies. In addition, no matter how contaminated seeds go through the germination process, the self-cleaning action to remove the toxic substances occurs to create the optimal medium conditions for the growth of mushroom mycelium.

The present invention, unlike the conventional method, by using only germinated brown rice or soybean as a medium raw material is difficult to absorb nutrients as in the prior art, it takes a long period of cultivation and overcomes the difficulty of waste medium treatment, mushrooms as well as medium used as a functional product It is to provide a way to make this possible.

Every mushroom has a different environment in which it grows, which results in different nutrients. As a mushroom medium, germinated brown rice and germinated soybeans are very useful for obtaining mushroom fruit bodies. Because. In general, mushroom fruiting bodies are parasitic from dead or decayed trees. In other words, it absorbs the nutrients of the wood and produces fruiting bodies. Here, mushrooms grow while absorbing the effects by softening / corrupting cellulose or semi-cellulose. Cellulose is very hard in tissue and takes considerable time to absorb nutrients.

After all, the same principle is applied to artificial cultivation of mushrooms using sawdust other than solid wood, and various attempts have been made to soften wood in artificial cultivation. Nevertheless, it takes considerable time and effort to soften solid wood.

In the present invention, the cultivation / cultivation using the germinated brown rice or germinated soybeans as a raw material of the mushrooms, rather than the wood / sawdust that takes a long time for decomposition and nutrient absorption, mushroom fruit body through a medium that is easy to absorb abundant nutrients Promotes the growth of, and the medium is also available as another functional product.

Germinated brown rice is sprouted up to 1-10mm by supplying proper moisture, temperature and oxygen to brown rice to maximize the nutrition and function of brown rice. When sprouted, phytic acid is changed to phosphorus and itositol, making it easier to break down, and when germinated, new substances such as vitamins, amino acids, enzymes, SOD, arabinoxyran and gamma-orizanol are produced.

In other words, the germination of grains and legumes creates new ingredients that have never been seen before, resulting in a completely new effect. The mushroom mycelium is easily decomposed and increased to double and double, making it a sufficient source of nutrition to fill fruiting bodies with sufficient nutrition. In addition, no matter how contaminated seeds go through the germination process, the self-cleaning action to remove the toxic substances occurs to create the optimal medium conditions for the growth of mushroom mycelium.

Moisture, nutrition, and absorption rate of the medium play a very important role in the growth of mushroom fruiting bodies. Germinated brown rice and germinated soybeans have optimized nutrition and moisture for mushroom growth, and no matter how weak the mushroom mycelium can absorb it. It is easy to disassemble so that the condition is prepared. In addition, sawdust and other nutrient sources contain a large amount of harmful ingredients that adversely affect the mushrooms, such as pesticides, heavy metals, dioxins, etc. These are very large inhibitors to the growth of mushroom mycelium. Germinated brown rice and germinated soybeans remove all of these harmful substances through their self-cleaning movements during germination, which greatly helps the growth of mushroom mycelium.

The present invention maximizes the absorption of the mushroom mycelium during artificial cultivation of the germinated brown rice or germinated soybeans, by eliminating harmful substances to shorten the harvesting period of the fruiting body of the mushroom, can be utilized by converting the waste medium into functional food The problem is that by providing a method, the cultivation period is shorter than that of the existing cultivation method, and a high profit is generated according to the useful commercialization of the waste medium.

Mushrooms that can be applied to the present invention can be liquid culture of the spawn and inoculum, and all mushrooms capable of inoculating liquid spawn are targeted.

To make germinated brown rice, first select the germinated brown rice with high germination rate.

After choosing brown rice with good germination rate, soak brown rice in water, then wash gently by rubbing. At this time, the rice eyes should not fall. The immaturity floats in the water, and the process of extracting it is repeated several times.

Wash soaked rice for 12 hours in 12-18 degrees of water and gradually raise the water temperature to 30 degrees. This process is repeated for 6 hours in the air, and the germination of 2-5mm is achieved, and the state can be used as a medium.

How to make germinated soybeans is immersed in 12-18 degrees of water for 12 hours, and then drained to keep moisture for 12 hours and germinate to about 10mm.

Put germinated brown rice or germinated soybeans in the growing bottle. The amount at this time is 50% of the bottle cap as an upper limit.

After the end of sickness, sterilize at 121 degrees in autoclave for up to 90 minutes, inoculate with liquid spawn, and incubate under the conditions of temperature, humidity and ventilation in the culture room under the conditions of each mushroom. Is grown and harvested in the growth room.

At this time, when the organic germanium or the active calcium is mixed in an appropriate amount in the water used during germination, it becomes a separate functional germinated brown rice having a high mineral content that is easily absorbed into the mushroom fruiting body and germinated brown rice.

[Examples and Comparative Examples]

An Example is shown below and it demonstrates more concretely. In addition, this invention is not limited to the following example.

Example 1

200 g of germinated brown rice was put in a 500cc P.P bottle and sterilized at 121 ° C for 40 minutes, and 10cc of liquid mushroom liquid spawn was inoculated. The culture was carried out in a culture chamber having a temperature of 23 degrees and a humidity of 70%.

It took 40 days for the mycelium to stick to the whole medium.

After sticking, the fungus was scraped, and the mushrooms were grown in a tree growing room at a temperature of 25 degrees and a humidity of 87%. Four weeks later, 70 g of the mushrooms were harvested from the germinated brown rice medium.

Example 2

200 g germinated long beans were put in a 500cc P.P bottle, sterilized at 121 ° C for 40 minutes, and 10cc of liquid mushroom spawn was inoculated. The culture was carried out in a culture chamber having a temperature of 23 degrees and a humidity of 70%.

It took 20 days for the mycelium to stick to the whole medium.

After sticking, the fungus was scraped, and oyster mushrooms were grown in a growth chamber with a temperature of 25 degrees and a humidity of 87%. After 20 days, 150 g of oyster mushrooms were harvested from the germination medium.

Comparative Example 1)

The culture and growth periods of the medium containing 500 g of larch sawdust and nutrients in 850cc P.P bottles and the flower mushroom medium containing 200 g of germinated brown rice were compared under the same conditions. The conditions at this time were as follows.

Sterilize at 121 ° C for 40 minutes. Inoculate approximately 10 g (cc) of liquid spawn on sawdust medium and germinated brown rice medium. Incubate in a culture room with a temperature of 23 degrees and a humidity of 70%. After the bacteria were scraped, they were grown in a growth room with a temperature of 25 degrees and a humidity of 87%.

As a result, the sawdust medium of zinnia mushroom took 60 days of culture and 40 days from growing room to harvesting, but 40 and 25 days of germinated brown rice medium, respectively.

At this time, the yield and pollution rate did not differ.

Comparative Example 2)

Germinated brown rice medium germinated with 0.5% of active (calcined) calcium (specific gravity ratio for water) and germinated brown rice medium germinated with general groundwater were germinated under the following conditions to compare the calcium content of leaf mushrooms.

200g each of the two kinds of germinated brown rice in 500cc P.P bottles, sterilized at 121 degrees for 40 minutes, and 5cc inoculated with liquid spawn. Cultivate in a culture room with a temperature of 21 degrees and a humidity of 70%. After the bacteria were scraped, they were grown in a growth room with a temperature of 23 degrees and a humidity of 85%.

As a result, 8.9 mg of calcium in 100 g of the germinated brown rice medium treated with activated calcium contained 1.7 mg of calcium in the germinated brown rice medium.

Example 3

150g germinated brown rice was put in a 500cc P.P bottle, and sterilized at 121 ° C for 60 minutes, and 5cc inoculated with leaf mushroom spawn. The culture was carried out in a culture chamber having a temperature of 21 degrees and a humidity of 70%.

It took 21 days for the mycelium to stick to the whole medium.

After sticking, the fungus was scraped, and the leaves were grown in a growth room with a temperature of 23 degrees and a humidity of 85%. After 4 weeks, 90 g of leaves were harvested from germinated brown rice medium.

Comparative Example 3)

Germination of germinated brown rice medium germinated with 0.01% germanium (specific gravity to water) and germinated brown rice germinated with general groundwater under the following conditions were compared to the germanium content of leaf mushrooms.

200g each of the two kinds of germinated brown rice in 500cc P.P bottles, sterilized at 121 degrees for 40 minutes, and 10cc inoculated with liquid spawn. Cultivate in a culture room with a temperature of 21 degrees and a humidity of 70%. After the bacteria were scraped, they were grown in a growth room with a temperature of 23 degrees and a humidity of 85%.

As a result, in the germinated brown rice medium treated with germanium, 6000 ppm of germanium in 100 g contained 250 ppm in normal germinated brown rice medium.

According to the present invention, artificial cultivation, which has been using wood or sawdust to date, is possible to grow mushrooms more simply and in a short time by using germinated brown rice or germinated soybean as a medium, and the conventional wastewater treatment required much time and labor. Unlike the method, the cost can be reduced.

In addition, these media can be used as a functional raw material, such as food, there is an effect that makes a significant contribution to the increase in profits.

Claims (4)

(a) immersing brown rice or soybeans in water and germinating to produce germinated brown rice or germinated soybeans; b) introducing the germinated brown rice or germinated soybeans at 23 to 50 (w / v)% based on the total volume of the mushroom cultivation bottle, and sterilizing at 121 ° C. for 40 to 90 minutes to prepare a mushroom cultivation medium; (c) inoculating a liquid spawn of a mushroom selected from the group consisting of zinnia mushroom, oyster mushroom and leaf mushroom in the medium of a); And (d) Cultivation method of mushrooms selected from the group consisting of zinnia mushroom, oyster mushroom and leaf mushroom including the step of culturing and growing at a temperature of 21 to 25 ℃, humidity 70 to 87% to harvest the mushroom. The method of claim 1, wherein the brown rice or soybeans are immersed and germinated in water further comprising 0.01 to 5% by weight of active calcium based on the total weight of water in step a). The method of claim 1, wherein the brown rice or soybeans are immersed and germinated in water further comprising 0.01 to 3% by weight of organic germanium based on the total weight of water in step a). Germinated brown rice or germinated soybean cultured with mycelia after harvesting the mushroom obtained by the method of any one of claims 1 to 3.