KR100482694B1 - Liposome base for trichogenousness, Cosmetic composition for trichogenousness containing the same, and Method for improving absorptance of natural ingredients for trichogenousness using the same - Google Patents

Abstract

The present invention relates to a hair growth promoting liposome base encapsulated with a herbal extract, a cosmetic composition comprising the same, and a method for improving the absorption rate of the herbal ingredient for promoting hair growth using the same, wherein the herbal extract is white porcelain, trademark herb, filamentous, sessau, white, and white skin It is characterized by containing one or more of licorice, wolfberry and yulmu extract. According to the present invention, the cosmetic composition for promoting hair growth contains the liposome base in which the herbal extract is enclosed as an active ingredient, thereby effectively delivering the active ingredient to the hair follicle, thereby improving the hair loss prevention and hair growth promoting effect of the herbal ingredient.

Description

Liposome base for promoting hair growth, cosmetic composition for promoting hair growth including the same and a method for improving the absorption rate of herbal ingredients using the same {and method for improving absorptance of natural ingredients for trichogenousness using the same}

In the present invention, nine kinds of plants that have been found to have a hair growth promoting effect, that is, white (Thujae orientalis), trademark (Mantidid ootheca), tortoise (Torilis Fructus), sewage (Polygonium multiflorum), mulberry (Broussonti kasinoki sieb), Mulberry (Mulberry) It relates to a liposome composition for promoting hair growth containing at least one of root bark), Glycyrrhiza, Lycium fructus and Coix seed extract. More specifically, the present invention is known to have a hair growth effect and the newly identified extract extracts the effect of inhibiting the 5-alpha-reductase, which promotes the production of the active male hormone dihydrotestosterone, which causes hair loss, and the nipple of hair follicles. The present invention relates to a liposome composition having the effect of effectively preventing hair loss and promoting hair growth by preparing liposome preparations with lecithin to contain such extracts, thereby efficiently delivering active ingredients to hair follicles. will be.

A baekjain (Thujae orientalis) is platycladus oval or oblong-in seeds (Thuja orientalis) or long circumferential length and 3 ~ 5㎜, diameter 2 ~ 3㎜. The outer surface is pale yellow to yellowish white, and the old one gradually changes to become yellowish brown, and oil leaks out. The outer surface of the seed is often enclosed by a thin membranous inner epithelium, the tip is usually pointed, and the round triad has dark brown spots. The lower part is a dull round and light in color. Microscopic examination of the cross-section of this drug is white to yellowish white, rich in endosperm, and rich in fat. Sweet, flat, nonpoisonous, and fragrant. The action relaxes the mind, helps the blood and stops sweating. The liver and kidneys are moisturized and the lower back is healed and healed. Baekjain has the efficacy of conscience of the god Anyang (안 心 安神) and Yun Jang Tongpyeon (潤腸 通便).

Brand seconds (Mantidid ootheca) is dangrang cattle, danpyo humble prayer, it steamed home insects that contain eggs mantidae (Mantidae). This herb is columnar to semicircular, with thin layers of several membranes arranged, stacked 24 to 45 mm long, 2 to 3 cm wide, and 15 to 20 mm thick. The outer side is pale brown to yellowish brown, the upper side is slightly raised, and the lower side is flat or has marks on the stem of the plant. The nature is sweet, salty, flat and nonpoisonous. It is used for elder oil well, establishment, uno incontinence, and abolition of menstruation.

Torilis Fructus is a fruit of the genus Torilis japonica Decanodolle, which is an elliptical standing genus that is flat with 2-4 mm in length and 1 mm in diameter. The outer surface of the branch is grayish yellow to gray brown with a small projection at the end and a thin fruit bottle at the bottom. The dorsal side has three solid lines and the suture surface is flat but there are two brown lines. Casualties are fragrant, taste is a little spicy and irritate the tongue. Man's genitals. It is used for weeping, gynecological, arthralgia, and hypothermia (introduction to oriental science).

Polygonium multiflorum ( Polygonium multiflorum ) is the root of the tuber of Polygonum multiflorum Thunb. The main action of the extract promotes the intestinal motility of the animal. In addition, there is a strong heart attack on the tired heart, inhibits serum cholesterol increase, and in vitro tests have a dose-dependent improvement in mycobacterium tuberculosis inhibitory activity and myocardial ischemic injury.

Paper mulberry ( Broussonti kasinoki sieb ) mainly contains saponin, fatty oil, serotonin , and paper. It is used as a fiber for making clothes, and it has the effect of moistening, diuresis, and blood circulation. It is used for the treatment of rheumatism, pain, bruises, swelling (cold edema), dermatitis, and circumferential lymphadenitis.

Mulberry root bark is the root bark of mulberry or other cognate plants, tubular, semi-tubular, or banded bark, 0.1-0.6 cm thick, white-orange, and sometimes white silk-like. Many of the fibers are exposed. Usually, hypotensive flavonoids are the mainstream, and it is known to have a blood glucose enhancing function (Taro Nomura et al, Planta Med, 1, 127 (1984)).

Licorice (Glycyrrhiza) is the same as Glycirrhiza glabra L., G. uralensis Fisher, or the same vegetation roots or gourds (skin licorice) or peeled. (Peel licorice) would. Extracts include hormonal and anti-inflammatory effects of anti-ulcerative adrenal cortex, hormonal and anti-inflammatory effects, estrogen-hormone action, detoxification, hyperlipidemia, and interferon organic activity. )

Lycium fructus is the fruit of the Lycium chinese Miller and removes fruit seeds and uses the shade. The water extract of goji berries shows a marked drop in blood pressure, inhibits the increase of lipids, and anti-interstitial and hepatic function protection. Recently, anti-aging, anti-arteriosclerosis, female hormone-positive action, anti-malignant tumor function, and hematopoietic function-promoting action are known, including promoting and regulating immune function. (1999), Culture and Culture History)

Coix seed is a seed removed from the seed of Coix lachryma-jobi L. var. Ma-yuen Stapf, which is characterized by the antitumor activity of coixenolide and the coix semen paste. It is known to have wart elimination effect due to abnormal keratin proliferation, and it is known to have analgesic, sedation, cough, antipyretic and insecticidal action by other ingredients.

In male expression, male impulse, skeletal muscle increase, male external genital development, scrotum growth, and spermatogenesis are involved in testosterone, and acne, sebum increase, hair loss and prostatic hyperplasia are related to dehydrotestosterone in the tissues. Known ( J. Invest. Dermatol . 1995; 105 (2): 209-14). Among male hormone-dependent diseases, male-type hair loss causes serious mental pain for men in the modern society, which focuses on beauty.

5alpha-reductase is present in testosterone, a testosterone biosynthesis in testes, present in testosterone-reactive tissues such as sebaceous glands, hair follicles, prostate, or epididymis, and dihydrotestosterone (DHT), an active testosterone. It is known to be a reductase involved in the conversion of NADPH as a coenzyme. Excessive secretion of dihydrotestosterone after puberty acts as a major causative agent in the production of sebum in sebocytes, causing acne and male hair loss. In particular, many hair follicle cells provide a cause for hair loss because dihydrotestosterone binds to hormone receptors better than testosterone. For this reason, studies are being actively conducted to develop a substance that inhibits the activity and production of 5-alpha-reductase, which promotes the production of dihydrotestosterone, and to use it as a hair regrowth or hair loss prevention agent.

Conventionally, female hormones such as estrogen have been used for the treatment of androgenetic alopecia, but female hormones have been reported to cause skin inflammation and side effects due to hormone administration. I'm using it. Recently, Merck in the United States has synthesized finasteride (finasteride, proscar), a drug that inhibits the action of 5 alpha-reductase and inhibits the production of dihydrotestosterone, and is commercially available as an oral administration hair regrowth, but is expensive and applied to the scalp If manufactured for direct application and the effect is ineffective and other side effects of oral administration occurs, there is a limit to use.

 Normally, the dermal papilla cells that form hair follicles play a role as mediators of several signaling systems in the central position that regulates the hair cycle and have special functions to control epidermal cell proliferation. In addition, the thickness of the hair is determined in proportion to the size of the dermal papilla in the hair follicle, that is, the number of dermal papilla cells, and is an important criterion for distinguishing the cilia and the straight hair. Therefore, hair growth agents for promoting proliferation of outer root sheath cells and dermal papillae cells among hair follicle cells have been developed (J Dermatol Sci 1994 Jul; 7 Suppl: S55-72).

There are three pathways through the skin: absorption through the stratum corneum, absorption through the hair follicle and sebaceous glands, and absorption through the eccrine gland. As far as is known, absorption through the stratum corneum is the most important absorption pathway. Since the stratum corneum is composed of nonpolar lipids in the stratum corneum, the polar substance is hardly absorbed and the lipophilic substance can be absorbed. have. In the case of a polar substance, it is combined with a protein part in the cytoplasm or interstices by a hydrogen bonding method and is not absorbed through the skin. Nonpolar substances, on the other hand, are primarily dissolved in the cell gap and sometimes cross the skin barrier. However, even through the stratum corneum, the solubility in the lower stratum corneum is less, it is prevented from being absorbed systemically by mechanisms such as enzymes that cause various metabolism and the exfoliation of keratinocytes. On this principle, the stratum corneum protects the body from numerous external polar substances, but nonpolar poisons can be absorbed into the stratum corneum. The greatest barrier to transdermal absorption is keratinocytes in the stratum corneum as described above and keratinocyte lipids therebetween. Since most of the active ingredients have a low percutaneous permeability due to the resistance of the stratum corneum, various methods are needed to overcome them and promote permeation of the active ingredients. The mixing of lipophilic substances with ethanol, propylene glycol, surfactants, fatty acids, etc. is a very effective method of transdermal delivery of components at the required speed and effective concentration. In addition, the use of liposomes made of lecithin or intercellular lipid components of the active ingredient is a much more effective method of transdermal absorption (Patent No. 2001-0083712).

Liposome preparations that began to emerge from the 70s as a means of penetration of drugs in the skin were observed, and corticosteroids and betamethasone were observed to be effective in skin permeation compared to general emulsion formulations (Crit Rev Ther). Drug Carrier Syst 1994; 11 (2-3): 97-118). From these results, an effective drug delivery system to hair follicles has been conducted by various research groups, and in fact, studies have been carried out to collect and deliver fluorescent dyes that can be distinguished by genes, proteins and the naked eye into hair follicles as liposomes. J Drug Target. 1998; 5 (2): 67-74). For example, when infiltrating hair follicles with melanin in the form of liposomes, the concentrations in the hair follicles increased with time but were not detected in the blood at all (J Dermatol Sci 1997; 14: 101-8). In another experiment, the lacZ gene was wrapped in liposomes and applied to rats, which showed selective expression only in the bulge area where hair matrix cells and stem cells are known. No other site was observed (Nature Medicine 1995; 1: 705-6). The particle size of liposomes was 60 nm to 600 nm, the smallest of which was accumulated in the stratum corneum, the largest size of 300 nm in the follicle depth (Int J Pharm 1994; 103: 277). Considering the fact that anti-androgen receptors are widely distributed in sebaceous glands and anti-androgen agents have limited effects and systemic side effects during oral administration, the infiltration of anti-androgen agents into liposomes in the form of liposomes is under study (Int J Pharm 1995; 126: 235.

Therefore, the present inventors tested 9 natural plant extracts for promoting hair follicle cell proliferation and 5 alpha-reductase inhibitory effects by in vitro test methods, and the natural extract of the present invention converts testosterone to dihydrotestosterone to reduce hair loss. It effectively inhibits the 5 alpha-reducing enzymes that induce and contains a large amount of active substances that promote hair follicle cell proliferation, so that it has an excellent hair growth promoting effect. The liposome base is prepared to contain such extracts and used for external skin preparations for hair growth promotion. The present invention has been completed.

Therefore, it is an object of the present invention to confirm that the nine kinds of extracts have an active ingredient for promoting hair growth, and encapsulated it in liposomes using lecithin and the like for the treatment of external skin treatment for promoting hair growth with improved absorption of the hair growth promoting active ingredient; It is to provide a cosmetic composition. In addition, it is revealed that the active ingredient of the natural plant extract has a 5 alpha-reductase inhibitory effect and promotes hair follicle cell growth and hair growth promoting effect, and by containing it as an active ingredient for preventing hair loss and promoting hair growth or hair growth. It is to provide a liposome cosmetic composition.

In order to achieve the above object, the present invention is an effective extract having the effect of inhibiting the 5 alpha-reductase and promoting the growth of dermal papilla cells, especially by heating and extracting nine natural herbs mixed with organic solvents, followed by heat extraction. It is characterized by the fact that it is a hair growth promoting cosmetic composition excellent in preventing hair loss and promoting hair growth by preparing liposomes with lecithin to contain the extract as an active ingredient.

In the composition according to the present invention, the natural extract is characterized in that it is encapsulated in the liposome base at a concentration of 0.0001 to 20% by weight based on the entire liposome base, the crude drug-packed liposome base 1 It is characterized by containing at a concentration of 30% by weight.

Hereinafter, the configuration of the present invention will be described in detail.

The five natural extracts of the present invention having a 5-alpha-reductase inhibitory effect and a hair follicle cell growth promoting effect and thus preventing hair loss and promoting hair growth are prepared in a form encapsulated in liposomes through the following steps.

(1) adding and mixing the organic solvent to the dried natural herbal medicine in a capacity of 2 to 5 times alone based on the dry weight of the herbal medicine;

(2) preparing a natural herbal extract by heating the natural herbal-organic solvent mixture in an extractor equipped with a cooling capacitor;

(3) concentrating the natural herbal extracts under reduced pressure in a reduced pressure evaporator with a cooling capacitor and completely drying the extract;

(4) characterized in that it is prepared according to the production method comprising the step of preparing a liposome by stirring the extract with lecithin, ethanol, ester oil, propylene glycol, vegetable oil, antioxidant and surfactant.

The nine kinds of white porcelain, trademarked herb, casualty, sewage, young mulberry, cedar, licorice, goji berry, and yulmu can be used as a herbal medicine.

As the organic solvent, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, hexane, hexane, ethyl ether or ethylacetate may be used. At this time, it can be extracted simply by using only one solvent of the organic solvent, it may be fractionally extracted using the organic solvent and water.

In the heating of (2), it is preferable to heat at 50 to 100 ° C. for 1 to 10 hours, but is not limited thereto. In addition to the above-described heat extraction, the natural herbal may be deposited in the organic solvent at 0 to 40 ° C. for 1 to 30 days to extract the active ingredient.

The liposome composition of the present invention at the preparation step (4) is a liposome cosmetic composition in which a natural herbal extract is encapsulated in a liposome stabilized using lecithin, ethanol, ester oil, propylene glycol, vegetable oil, antioxidant and surfactant. . Preferably, the present invention is a liposome base prepared by adding a natural herbal extract with 2 to 8% by weight of lecithin, 10 to 15% by weight of ethanol, 10 to 30% by weight of ester oil, 5 to 10% by weight of propylene glycol and purified water. For example, based on the total weight of the composition, liposome base can be applied to the hair or skin at a concentration of 1 to 30% by weight, softening lotion, nourishing cream, nutrition cream, massage cream, essence, pack, hair tonic, hair lotion It can be used in addition to formulations such as shampoos and tonics.

The herbal extract prepared by the above method was found to have an excellent inhibitory effect of 5-alpha-reductase through a test using 5-alpha-reductase obtained from the prostate of rats. In addition, hair growth effect test using a mouse along with hair follicle proliferation effect was found to have a significant hair growth promoting effect.

At this time, the amount of the nine natural herbal extracts to be added may be added to the liposome base in an amount of 0.0001 to 20% by weight, and preferably 0.001 to 5% by weight, based on the stability of the base to the entire liposome base.

The liposome cosmetic for promoting hair growth of the present invention is formulated in an amount of 0.001 to 30% by weight of the liposome base encapsulated in the above-mentioned medicines can be applied to the hair or skin, supple cosmetics, nourishing cosmetics, nutrition cream, massage cream, essence, It can be prepared and used in formulations such as packs, hair tonics, hair lotions, shampoos and tonics.

Hereinafter, the configuration of the present invention will be described in detail by the production examples and test examples, but the scope of the present invention is not limited thereto.

Preparation Example 1 Preparation of 9 Herbal Extracts

1) 250ml of 70% ethanol (ethanol) was added to 100 g of the dried herbal medicine, which was boiled and extracted for about 2 hours in an extractor equipped with a cooling capacitor, filtered to remove the residue, and an extract was prepared. The extract was concentrated under reduced pressure at 60 ^° C. using a distillation apparatus equipped with a cooling capacitor to obtain dried herbal extract I.

2) 250 mL of hexane (hexane) was added to 100 g of the dried herbal medicine, which was boiled and extracted for about 2 hours in an extractor equipped with a cooling capacitor, and then filtered to remove the residue, thereby preparing an extract. The extract was concentrated under reduced pressure at 60 ^° C. using a distillation apparatus equipped with a cooling capacitor to obtain dried herbal extract II.

3) 200 ml of ethyl acetate (ethylacetate) was added to 100 g of the dried herbal medicine, which was boiled and extracted for about 2 hours in an extractor equipped with a cooling capacitor, filtered to remove the residue, and an extract was prepared. The extract was concentrated under reduced pressure at 60 ^° C. using a distillation apparatus equipped with a cooling capacitor to obtain dried herbal extract III.

Preparation Example 2 Preparation of Liposomes Containing 9 Herbal Extracts

2.5% by weight of the herbal extract prepared as described in Preparation Example 1, 2.5% by weight of lecithin, 13% by weight of ethanol, 25% by weight of ester oil, 7% by weight of propylene glycol, 0.1% by weight of nonionic surfactant and purified water Homogenized at 3000 ° C. at 70 ° C. for 10 minutes. Subsequently, the homogenized lecithin mixture was treated once with a microfludizer at about 70 at about 1,000 atm to form a liposome base. Using the liposome base formed as described above to prepare a liposome base encapsulated effective extract.

In order to compare the efficacy of the liposome base and the herbal extract prepared in this way, the final concentration was used in dilution in purified water, and a test example was carried out according to the following method in order to find the 5 alpha-reductase inhibitory effect.

Experimental Example 1 Test of 5-alpha-Reductase Inhibitory Effect Using Sprague Dawley Prostate

7-8 weeks old male Sprague-Dawley was killed with diethylether and the abdominal prostate was removed to remove connective tissue. Buffer (0.32M sucrose, 0.1 mM dithiothreitol, 20 mM sodium acetate) was added thereto, chopped finely by a grinder and suspended. The suspension was filtered with gauze, and the filtrate was centrifuged at 1500 × g, pellets were taken, and the buffer solution was mixed and suspended at a concentration of 20-30 mg / ml.

After taking a portion of the suspension was placed in a 0.02M phosphate buffer, it is reacted with a substrate which is a testosterone isotopes ³ H attached to measure the amount of dihydrotestosterone product. At this time, the reaction solution is dithiothreitol, 20mM of phosphate buffer (pH 6.5) in 1mM, of 50μM NADPH, [1,2,6,7- ³ H] testosterone 0.1μCi / testosterone (1 × 10 ^-7 M ) And 36 μg enzyme suspension were added to prepare a total reaction solution of 565 μl.

The above-mentioned herbal extracts I-III of the present invention and a certain amount of liposome base were dissolved in 10% dimethyl sulfoxide (DMSO) and purified water, respectively, and added to 10 μl per reaction, and the same volume of solvent was used as a control and finasteride was used as a positive control. (finasteride) was used.

In addition, the reaction was carried out at 37 ° C. for 30 minutes by adding the prepared enzyme suspension. Then, 600 µl of ethyl acetate was added to stop the reaction, and the product was dried through nitrogen gas. This is a drip on the total amount of ethyl acetate to the silica plastic sheet kayijel gel 60 F was dissolved in ₂₅₄ of 50㎕ (Silica plastic sheet kieselgel 60 F ₂₅₄₎ and then a developing solvent ethyl acetate: (1: 1) cyclohexane (cyclohexane) Developed using.

After drying the silica plastic sheet in the natural state, a bath (Bas, FUJIFILM, Japan) system was used to measure the amount of isotopes, and the dried plastic sheet and the exposed film were put together in a bath cassette and stored for one week. The isotope amounts of testosterone and resulting dihydrotestosterone exposed to the film were then quantified using the exposed intensity.

The experiment was used repeatedly for two times, and based on the measurement result, each of 5 alpha-reductase inhibition rates was calculated by the following calculation method. The results of the 5 alpha-reductase inhibition of the liposome base according to the present invention are shown in Table 5 and FIG. 2.

As described in the above results, the nine herbal extracts of the present invention had a 5 alpha-reductase inhibitory effect, and each of the nine encapsulated liposome bases (Preparation Example 2) was the extract itself (Preparation Example 1). The inhibitory effect was found to be higher.

Test Example 2 Test for promoting the proliferation of dermal papillae cells in hair follicle cells

Human hair follicle dermal papilla cells cultured in DMEM (Dulbecco's Modified Eagle's Media) medium containing 2% fetal bovine serum were dispensed in a 96-well microtiter plate at 5,000 cells / well, and the sample 9 was used as a sample. The herbal extract of eggplant and each of the encapsulated liposome bases were added by diluting sequentially by 1/10 with a culture medium so that the concentration of the herbal extract was finally 0.01%, and then incubated at 37 ° C. for 4 days. After incubation, 50 μl of 0.2% MTT (3- [4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide) solution was added to each well, followed by incubation at 37 ° C. for 4 hours. The resulting formazan was dissolved in dimethyl sulfoxide (DMSO). The absorbance of the dissolved formazan was measured at 570 nm using a microplate reader. This was carried out in the same manner as above for the control to measure the absorbance. After comparing the absorbance of each of the nine herbal extracts and the experimental group containing each of the encapsulated liposome base and the control group without the same, the results are shown in Table 2 and FIG. 3.

As shown in Table 2 and FIG. 3, nine herbal extracts showed growth promoting activity of one of the hair follicle cells, one of the hair follicle cells at a constant concentration (0.01%), and the herbal extract was encapsulated with a liposome base ( Preparation Example 2) confirmed that the dermal papilla cell proliferation ability was higher than that of the extract itself (Preparation Example 1).

Experimental Example 3. Test of hair growth effect of herbal extract and its liposome base through mouse

The nine extracts of the present invention prepared above and their respective encapsulated liposome bases were prepared in 30% hydrous ethanol solution with the same concentration of 2% final extract, and the liposomes were diluted in purified water. The hair growth effect of the inventive herbal extract and its liposome base was tested.

Remove the back hair of 47-53 days old mouse (C57BL / 6), select the clean back skin, and select 10 animals for each substance group, and 100 μl of the test extract and liposome base dilution solution of the present invention daily. Applied.

The length of hair and the extent of hair growth over time were compared by adding scores from 0 to 3 according to the degree of restoration after hair removal. In order to compare the degree of hair growth, the control group was applied with 30% alcohol solution to each individual to observe the hair growth state. The result is shown in Table 3, FIG. 4A and FIG. 4B.

Test substance Elapsed days 10 days 20 days Negative Control (ethanol) 0.25 ± .25 1.42 ± .30 Positive control group (minoxidil) 1.22 ± .24 2.95 ± .47 White porcelain extract (Preparation Example 1) 1.12 ± .45 2.85 ± .52 Cranberry Extract (Manufacturing Example 1) 1.25 ± .38 2.95 ± .54 Casualty extract (Manufacturing example 1) 0.85 ± .43 2.50 ± .40 Sewage Extract (Manufacturing Example 1) 1.05 ± .41 2.75 ± .62 Paper mulberry (Manufacturing Example 1) 0.76 ± .25 1.86 ± .34 Cereals Extract (Preparation Example 1) 0.73 ± .36 1.79 ± .46 Licorice Extract (Manufacturing Example 1) 0.62 ± .19 1.46 ± .43 Wolfberry Extract (Manufacturing Example 1) 0.81 ± .32 2.67 ± .57 Yulmu Extract (Manufacturing Example 1) 0.98 ± .32 2.75 ± .39 White porcelain extract encapsulated liposome base (preparation example 2) 1.35 ± .26 2.97 ± .32 Liposome Base Encapsulated with Trademark Extract (Manufacturing Example 2) 1.50 ± .42 2.95 ± .23 Casualty extract packed liposome base (Preparation Example 2) 1.23 ± .15 1.95 ± .40 Sewage Extract Packed Liposome Base (Preparation Example 2) 1.65 ± .43 2.80 ± .42 Lilacsome Base Encapsulated with Saccharaceae Extract (Preparation Example 2) 1.20 ± .34 2.20 ± .42 Limesome base encapsulated with lettuce extract (Preparation Example 2) 1.13 ± .42 2.30 ± .50 Licorice Extract Packed Liposome Base (Preparation Example 2) 0.93 ± .38 1.65 ± .49 Wolfberry extract encapsulated liposome base (preparation example 2) 1.34 ± .45 2.80 ± .24 Yulmu Extract Encapsulated Liposome Base (Preparation Example 2) 1.10 ± .40 2.80 ± .45

In the above results, in the case of the nine kinds of extract-containing agent of the present invention according to the above preparation it was confirmed that there is a significant hair growth promoting effect, the liposome base (Preparation Example 2) each of the extract is the extract itself (Preparation Example It confirmed that the hair growth was promoted higher than 1).

The liposome base encapsulated with the herbal extract was intended to be used as a skin external preparation for promoting hair growth in an amount of 1 to 30% by weight based on the total weight of the composition. However, the scope of the present invention is not limited to the following formulation examples.

Formulation Example 1. Nutritional lotion

ingredient weight% Liposome Base with Herbal Extract 5.0 Beeswax 4.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.7 Liquid paraffin 5.0 Squalane 5.0 Caprylic / Capric Triglycerides 5.0 glycerin 3.0 Butylene glycol 3.0 Propylene glycol 3.0 Carboxy Vinyl Polymer 0.1 Triethanolamine 0.2 antiseptic Quantity Pigment Quantity Spices Quantity Purified water balance system 100.0

Formulation Example 2. Hair Tonic

ingredient weight% ethanol 55.0 Castor Oil 5.0 glycerin 3.0 Pyrotonamine 0.1 Liposome Base with Herbal Extract 1.0 Spices Quantity Purified water balance system 100.0

Formulation Example 3. Hair Lotion

ingredient weight% Cetostearyl alcohol 2.0 Stearyl Triethyl Ammonium Chloride 2.0 Hydroxyethyl cellulose 0.5 Pyrotonolamine 0.1 Liposome Base with Herbal Extract 10.0 Spices 0.5 Purified water balance system 100.0

Formulation Example 4. Hair Soap

ingredient weight% Liposome Base with Herbal Extract 5.0 Titanium dioxide 0.2 Polyethylene glycol 0.8 glycerin 0.5 Ethylenediaminetetraacetic acid  0.05 salt 1.0 Pigment Quantity Soap Quantity Toilet soap base (13% moisture, parts by weight) to 100 system 100.0

As shown in the preparation and test examples above, the liposome base in which nine herbal medicines were heated and fractionated with an organic solvent and the concentrated herbal extract was encapsulated in a liposome composed of lecithin has an excellent 5-alpha-reductase inhibitory effect. In addition, since it has an excellent effect of promoting hair follicle cell proliferation and promoting hair growth, it may be usefully used as a medicine or cosmetic for promoting hair growth and hair loss treatment when preparing an external preparation for skin. On the other hand, it can be used as a therapeutic agent or cosmetic for treatment of acne, seborrheic dermatitis or dandruff due to excessive sebum secretion. It can also be used for the treatment of various diseases involved.

Figure 1 shows the effect and delivery process of the male hormone in the hair cells.

Figure 2 is a graph showing the 5 alpha-reductase inhibitory effect of the extract preparation of the present invention containing the hair growth promoting active ingredient.

Figure 3 is a graph showing the effect of promoting the proliferation of dermal papilla cells in human hair follicle cells extracts containing hair growth promoting active ingredient.

4A and 4B are graphs showing the results of testing hair growth effects using C57BL / 6 mice for hair growth promoting effective plant extracts and liposome compositions thereof.

Claims (8)

delete Herbal extract that induces hair growth by inhibiting 5 alpha-reductase and promoting hair follicle cell proliferation. Liposome base for promoting hair growth with an extract. 3. The liposome base for promoting hair growth according to claim 2, wherein the herbal extract is encapsulated in the liposome base at a concentration of 0.0001 to 20% by weight based on the entire liposome base. The liposome base for promoting hair growth according to claim 2, wherein the liposome base contains lecithin. 5. The liposome base for promoting hair growth according to claim 4, wherein the liposome base comprises lecithin, ethanol, ester oil and propylene glycol. A hair cosmetic composition for promoting hair growth comprising the liposome base for promoting hair growth according to any one of claims 2 to 5 in a concentration of 1 to 30% by weight based on the whole composition. The hair cosmetic composition for promoting hair growth according to claim 6, wherein the hair cosmetic composition for promoting hair growth is a flexible cosmetic water, a nourishing cosmetic water, a nourishing cream, a massage cream, an essence, a pack, or a soap.         A method for improving the absorption rate of a herbal ingredient for inhibiting 5 alpha-reductase and promoting hair follicle cell proliferation, wherein the liposome base for promoting hair growth according to any one of claims 2 to 5 is used.