KR20070091482A - Cosmetic composition for protecting sebum secretion on the skin - Google Patents

Abstract

A sebum secretion inhibiting cosmetic composition is provided to be effective for inhibiting the sebum excessively secreted from skin by inhibiting the activity of 5alpha-reductase which is an enzyme for catalyzing the formation of dehydrotestosterone known to be inducing the sebum secretion. A cosmetic composition for inhibiting sebum secretion comprises an extract of Crataegus pinnatifida(hawthorn), an extract of Parthenocissus tricuspidata(Ivy) and an extract of Syzygium aromaticum(clove), where the total content of extracts regarding the weight of the composition is 0.001-10 wt.%.

Description

Cosmetic composition for inhibiting sebum secretion {Cosmetic composition for protecting sebum secretion on the skin}

1 is a graph showing the results of 5α-reductase activity inhibition experiment of the present invention.

2 is a graph showing the results of sebum secretion inhibition experiment of the present invention.

The present invention relates to a cosmetic composition for inhibiting sebum containing Hawthorn (Hawthorn) extract, Ivy (Ivy) extract and Clove (Clove) extract. The natural extracts of the sebum inhibiting cosmetic composition inhibit the activity of 5α-reductase, an enzyme that catalyzes the production of dihydrotestosterone, which is known to induce sebum secretion, and thus has a useful effect in inhibiting sebum secreted excessively in the skin. Indicates.

In general, sebum in the skin including the scalp and face is responsible for maintaining the skin's moisturizing or microorganism invasion, but after puberty will promote acne. In particular, women in their 30s and older may cause problems such as skin swelling, makeup lifting, and pore enlargement due to excessive secretion of sebum. There are various causes for the excessive secretion of sebum, but it is known that the sebaceous gland secretion is caused by the activation of the cells of sebaceous gland by the amount of dihydrotestosterone, one of the hormones involved in promoting sebum secretion in the activity of sebaceous gland have.

5α-Reductase is present in testosterone-reactive tissues such as sebaceous glands, hair follicles, prostate and epididymis, reducing testosterone, one of the testosterones, to Dihydrotestosteron (DHT). As an enzyme involved, its conversion requires nicotinamide adenine dinucleotide phosphate (NADPH). Testosterone is involved in skeletal muscle growth and sperm formation, and dihydrotestosterone is involved in tissues in acne, sebum and prostatic hyperplasia (Diane et al; J. Invest Dermatol. 775-778, 1995. Bruchovsky, N et al; JBC 243, 2112-2121, 1968). In addition, in the sebaceous glands of the skin, testosterone is converted to dihydrotestosterone by 5α-reductase, binds to a receptor protein in the cytoplasm, enters the nucleus, activates sebaceous gland cells, and stimulates differentiation, thereby over secreting sebum in sebaceous glands. (Diane et al; J. Invest Dermatol. 105; 209-214).

Therefore, the present inventors have studied a substance capable of suppressing the excessive secretion of sebum in order to solve the problems such as the excessive secretion of sebum, and as part of this research, an effective substance among natural substances that have already been proven safe. As a result, Hawthorn extract, Ivy extract and Clove extract had inhibitory effect of 5 α-reductase and lipid biosynthesis, and the extracts were mixed and used simultaneously. The present invention was completed by discovering that the effect is much better and thus suppressing excessive secreted sebum more effectively.

Accordingly, it is an object of the present invention to provide a cosmetic composition that can more effectively prevent or treat sebum-related diseases by inhibiting sebum that is excessively secreted of the three natural extracts mentioned above.

In order to achieve the above object, the present invention provides a cosmetic composition for inhibiting sebum containing Hawthorn (Hawthorn) extract, Ivy (Ivy) extract and Clove (Clove) extract.

Hereinafter, the present invention will be described in more detail.

Hawthorn (Hawthorn) is a rosaceae, scientific name is the rosacea rosea (Crataegus pinnatifida Bge., C. pinnatifida Bge.var.major N.E.Br. or C.suneata Sieb. Et Zucc). The main components of the fruit are creagoline acid, citric acid, tartaric acid, flavones, sugars, glycosides, vitamin C and the like. Hawthorn is commonly used to stimulate digestion and promote stomach functional activity. It can also improve normal blood circulation and relieve congestion. (Korean Patent Publication, 10-2004-0043092)

Ivy (Ivy) is a deciduous broad-leaved vine plant belonging to the dicotyledon buckthorn vine family and grows on stone walls, rocks or tree trunks. The stem grows more than 10m when fully grown, and the leaves are 10-20cm wide, egg-shaped, pointed at the tip of the leaf, split into 3, the bottom is heart shaped, hairless on the front, fine hair on the leaf vein on the back, There are irregular sawtooth, petioles longer than leaves. Flowers are bisexual, yellow-green in June-July, with a large number of peduncles from the end of branches or axillary. Fruits are covered with white powder, 6-8mm in diameter, ripen in August-October. These ivy are known to relieve blood in one shot and to relieve pain in joints and muscles.

Clove is the scientific name Eugenia caryophyliate, and its main components are Linalyl acetate, Linalool, and Eugenol. And strong nervous system sedation effects.

The three extracts may be an extraction operation such as solvent extraction, steam distillation or extraction by carbon dioxide using a supercritical extraction technique.

To control the amount of dihydrotestosterone known to induce sebum secretion, the present inventors sought to find a natural extract that inhibits the activity of 5α-reductase, an enzyme that catalyzes the production of dihydrotestosterone. Experiments have shown that inhibition of 5α-reductase activity in vitro shows that Hawthorn, Ivy and Clove extracts act as inhibitors of 5α-reductase. Found.

And, it was found that the best effect when all three extracts are contained (Test Example 1).

In addition, it was found that sebum secretion on the face was reduced when 10 men and women who felt that there was a lot of sebum secretion were applied to the face by applying the cosmetic composition containing the three extracts (Test Example 2).

Therefore, the present invention intends to use the extracts of Hawthorn, Ivy and Clove to more effectively reduce the inhibition and sebum secretion of 5α-reductase.

The extract is preferably contained 0.001 ~ 10% by weight based on the total weight of the cosmetic composition. This is because when the total content of the extract is less than 0.001% by weight, the sebum inhibitory effect is insignificant, and when the total content of the extracts is greater than 10% by weight, it has a great influence on the feeling of cosmetic use.

Hereinafter, although an Example and a test example are given and this invention is demonstrated in detail, this invention is not limited only to these examples.

Preparation Example 1 Preparation of Hawthorn Extract

200g dry weight hawthorn fruit is extracted using 50% 1,3-butylene glycolic acid solution. This was concentrated again to obtain 2 g of hawthorn extract.

Preparation Example 2 Preparation of Ivy Extract

382 g dry weight ivy is extracted using 60% ethanol. This was concentrated again to obtain 1.79 g of ivy extract.

Preparation Example 3 Preparation of Clove Extract

Using a glass container, 600 g of clove powder is immersed in a 5-fold volume of ethyl acetate and left for 1 week with occasional stirring at room temperature and in a light-shielded state. The mixture is filtered twice on filter paper to remove powder and to obtain an extract. The extract was concentrated under reduced pressure to remove the solvent, and 48 g of clove extracts were obtained.

Example 1 Flexible Lotion

The sum of the hawthorn extract, ivy extract and clove extract prepared in Preparation Examples 1 to 3 contained a total of 3.00% by weight, thereby preparing flexible cosmetics with the composition shown in Table 1 below.

          ingredient Content (% by weight)  Hawthorn + Ivy + Clove Extract 3.00  Polysorbate 1.50  Sorbitan sesquioleate 1.00  ethanol 8.00  Caprylic / Capric Triglycerides 4.00  glycerin 5.00  Butylene Glycol 5.00  Propylene glycol 3.00  antiseptic Quantity  Pigment Quantity  Spices Quantity  Purified water To 100

Test Example 1 Inhibitory Effect of 5α-Reductase Activity

Mature Sprogue-Dawley male rats (7-8 weeks old) were killed with diethyl ether, and then the abdominal prostate was removed to remove connective tissue and buffered (0.32 M sucrose, 0.1 mM dithiothreitol). (dithiothreitol), 20 mM sodium acetate, was added to finely chopped and suspended in a grinder. The suspension was centrifuged to obtain supernatant, partially purified 5α-reductase, and the activity inhibition experiment of this enzyme was performed.

Taking a portion of the supernatant obtained above was placed in a buffer containing 0.2M monobasic acid and 0.2M dibasic acid, is reacted with isotope substrate (testosterone) marked with ³ H measure the amount of dihydrotestosterone product It was. The reaction solution was added 1 mM dithiothreitol, 40 mM sodium phosphate (pH 6.5), 50 μM NADPH, [1,2,6,7-3H] testosterone / testosterone and enzyme suspension (0.8 mg) to a total volume of 565 μl. It was.

The sample was dissolved in 10% ethanol to 0.1%, and then 5.65 μl of the above reaction solution was added so that the final concentration was 0.001%. The same volume of solvent was used as a control group, and finasteride was used as a positive control group. Finasteride is a well known inhibitor of 5α-reductase and is approved by the US FDA. Currently, Merck, USA, manufactures and sells a product called Propecia, which has an excellent 5α-reductase effect.

The reaction was started at the same time as the addition of the enzyme suspension and proceeded at 37 ° C. for 30 minutes, followed by extraction with 1 ml of ethyl acetate. 100 μl of ethyl acetate was extracted on silica plastic sheet kieselgel F254. The developing solvent system was developed with acetate-cyclohexane (1: 1).

After drying the plastic sample in air, a bath system was used to measure the isotope amount. The dried plastic sheet and the x-ray film were put together in a bath cassette, and after one week the copper member of testosterone and dihydrotestosterone remained on the film. The quantification was measured.

The results for each inhibition rate are shown in Table 2 below. On the other hand, in the samples of the following Table 2, the mixing ratio of each preparation example is 1: 1.

  Sample (0.001%) T (dpm) DHT (dpm) % Conversion % Inhibition  Preparation Example 1 7680 2105 25.3 47.3  Preparation Example 2 8450 2750 26.3 45.2  Preparation Example 3 7540 2300 24.8 48.3  Preparation Example 1 + Preparation Example 2 6980 1890 23.1 51.9  Preparation Example 1 + Preparation Example 3 8130 2010 21.2 55.8  Preparation Example 2 + Preparation Example 3 6835 1840 19.1 60.2  Preparation Example 1 + Preparation Example 2 + Preparation Example 3 9105 2990 17.1 64.4  Control 7430 5935 48.0 -  Positive control group 7500 2530 29.9 37.7

(1) T: 3H Radio activity in the testosterone region

(2) DHT: 3H radioactivity in the dihydrotestosterone region

(3) Conversion rate: radioactivity to the DHT region / total radio activity

(4) Inhibition rate: 100 * (control conversion-sample conversion) / control conversion

From the results of the above [Table 2], when mixing all Hawthorn extract, Ivy extract and Clove extract, 5α-reductase inhibitory effect than when containing the extract individually Could see much better.

Test Example 2 Sebum Secretion Inhibitory Effect

Ten subjects men and women who felt that there was a lot of sebum secretion were selected, and according to the prescription according to [Table 1] of Example 1, a lot of sebum of the face was corrected every day for four weeks.

   Subject Pre-test amount (μg / cm ² h)     After 2 weeks of exam     After 4 weeks of exam One 76 65 62 2 69 64 53 3 94 85 79 4 101 94 87 5 86 80 75 6 75 69 61 7 69 65 58 8 74 66 60 9 65 56 50 10 59 51 45

From the results in Table 3, it can be seen that the cosmetic composition according to the present invention can effectively inhibit sebum.

As described above, sebum secretion cosmetic composition containing Hawthorn extract, ivy (Ivy) extract and Clove extract at the same time is very safe when applied to the human body while reducing the sebum secretion effect Since it is excellent, it can be usefully used for the prevention or treatment of sebum-related diseases such as seborrheic dermatitis, enlargement of pores by acne.

Claims (4)

A cosmetic composition for inhibiting sebum secretion, comprising hawthorn extract, ivy extract, and clove extract. A cosmetic composition for inhibiting 5α-reductase activity, comprising hawthorn extract, ivy extract, and clove extract. The cosmetic composition of claim 1 or 2, wherein the cosmetic composition contains a total of 0.001-10% by weight of Hawthorn extract, Ivy extract and Clove extract based on the total weight of the composition. Cosmetic composition to be. The cosmetic composition according to claim 1 or 2, wherein the cosmetic composition is a softening cream, a nutrient cream, a massage cream, a nourishing cream, a pack, a gel, an essence, a lipstick, a makeup base, a foundation, a lotion, an ointment, a gel, a cream, a patch or Cosmetic composition, characterized in that formulated as a spray.

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