KR20130115497A - Cosmetic composition containing fermented extracts of panax ginseng root, ganoderma lucidum and white tea and method for preparing the same - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Panax ginseng roots, Ganoderma lucidum, and silver needle tea is provided to ensure solubility, affinity, and stability without skin irritation and to increase antioxidation, collagen synthesis, and inflammation inhibition. CONSTITUTION: A cosmetic composition contains 0.01-90 wt% of fermented extracts of Panax ginseng roots, Ganoderma lucidum, and silver needle tea as active ingredients. The fermented extracts are prepared using one or more bacteria selected among yeast, lactic acid bacteria, and Bacillus sp. The yeast is Saccharomyces cerevisisae. A method for manufacturing the cosmetic composition comprises the steps of: culturing the Panax ginseng roots, Ganoderma lucidum, and silver needle tea with the bacteria; and extracting the fermented Panax ginseng roots, Ganoderma lucidum, and silver needle tea. [Reference numerals] (2',7') Intensity of dichlorofluorescein (comparative example contrast%); (AA) Comparative example 1; (BB) Example 1; (CC) Example 2; (DD) Example 3

Description

Cosmetic composition comprising a fermented extract consisting of Cheonsam, Nokgakyoungji, and Baekhoeun Acupuncture as an active ingredient, and a method for producing the same, and a method for preparing the same.

The present invention relates to a cosmetic composition comprising a fermented extract and a method for producing the same.

Human skin is constantly changing with age. Skin aging is largely divided into natural aging (or endogenous aging) and external aging. Natural aging (endogenous aging) is difficult to artificially control because it is influenced by genetic factors, whereas external aging is caused by environmental factors. It is relatively easy to adjust. Representative external aging factors include ultraviolet rays, the most prominent external aging phenomenon is wrinkle formation.

On the other hand, the skin exposed to ultraviolet rays generates free radicals and reactive oxygen species (ROS), causing skin cancer, phototoxicity, autoimmune disorders, photosensitivity and skin aging. Synthesis and degradation of extracellular matrix such as collagen in vivo is properly controlled, but its synthesis decreases as aging progresses, and the expression of matrix metalloproteinase (MMP), an enzyme that breaks down collagen, is promoted, resulting in decreased skin elasticity. And wrinkles are formed.

On the other hand, atopic dermatitis is a representative disease in people with atopic dermatitis. Although the cause of atopic dermatitis has not been clarified yet, about 70% of patients with atopic dermatitis have a history of atopic dermatitis and allergic predisposition. It is known to be one of the most common allergic diseases, such as allergic rhinitis, asthma, etc., is understood as one of the genetic factors and immune diseases. In the treatment of atopic dermatitis, corticosteroids such as corticosteroids are expected to have high pharmacological effects. However, side effects of corticosteroids have become a problem. It is also well known that a dramatic aggravation of lesions occurs later. As a countermeasure for such side effects, nonsteroidal anti-inflammatory agents or antihistamines, which do not contain hormones such as corticosteroids, are used, but since they are difficult to cure, it is very urgent to reduce or treat atopic dermatitis without side effects.

Recently, with respect to functional cosmetics having functions such as antioxidants and whitenings obtained from natural extracts to reduce skin irritation by various chemicals, Korean Patent Application Publication No. 2011-0041178 discloses germinated soybean fermented extract and germinated sesame fermented extract. Regarding the anti-aging effect of the cosmetic composition containing a mixture of, Korean Patent Laid-Open Publication No. 2011-0003872 discloses the antioxidant and whitening effects of the fermented extract of the complex plant extracted by combining green tea, lettuce, licorice mixed fermentation and fermented ginseng. It is starting.

However, there is a problem that the cosmetic composition that satisfies the antioxidant effect, collagen synthesis effect and the inflammatory response inhibitory effect at the same time, and which contains a high content of an active ingredient effective in antioxidant, collagen synthesis and inflammatory response inhibition is not obtained.

Republic of Korea Patent Publication No. 2011-0041178 Republic of Korea Patent Publication No. 2011-0003872

Therefore, the cosmetic composition comprising the fermented extract according to the present invention and a method for preparing the same include fermented extracts of Cheonsam, Noggak ganoderma lucidum and white tiger, as effective ingredients, which can increase the antioxidant effect, collagen synthesis effect, and inflammatory response inhibitory effect. It is an object of the present invention to provide a cosmetic composition comprising a fermented extract and a method for producing the same.

According to a feature of the present invention for achieving the above object, the present invention provides a cosmetic composition comprising a fermented extract of Cheonsam, Noggak ganoderma lucidum and white tiger roe needles as an active ingredient.

In addition, the present invention comprises the steps of culturing the one or more bacteria selected from the group consisting of yeast, lactic acid bacterium and white tiger silver needles yeast, lactic acid bacteria and Bacillus genus; And it provides a method for producing a cosmetic composition comprising the step of extracting the fermentation extract of Cheonsam, Noggak ganoderma lucidum and white tiger.

Therefore, the cosmetic composition comprising the fermented extract of the present invention and a method for preparing the same according to the above problem solving means have no skin irritation according to including the fermented extract of Cheonsam, Noggak ganoderma lucidum and white tiger rim as an active ingredient, cosmetic base, additives And solubility, affinity, and stability in organic solvents and the like, and particularly, have an effect of increasing antioxidant, collagen synthesis, and inhibition of inflammatory reaction.

1 is a graph showing the intensity of 2 ', 7'-dichlorofluorescin (2', 7'-dichlorofluorescin, DCF) of Examples 1 to 3 and Comparative Example 1.
2 is a graph showing the release amount of beta-hexosaminidase of Examples 1 to 3 and Comparative Example 1.
Figure 3 is a graph showing the concentration of procollagen type I C-terminal peptide (PIP) of Examples 1 to 3 and Comparative Example 1.

Hereinafter, the cosmetic composition comprising the fermented extract according to the present invention and specific details for carrying out the preparation method thereof will be described.

The present invention provides a cosmetic composition comprising a fermented extract of Cheonsam, Noggak ganoderma lucidum and white tiger rim as an active ingredient.

Cheonsam used in the present invention refers to the red ginseng of the highest grade, which is pale yellow brown in appearance and has a root shape in the shape of a human being in harmony with the head, torso, and leg. Cheonsam's internal tissues are dense and without any signs of illness, and when the base of the head is cut diagonally, there should be no internal pores and no whites. This is because internal tissues must be dense and contain large amounts of active ingredients such as saponins.

The nocturnal ganoderma lucidum is named after the shape of the ganoderma lucidum fruit body resembles the horn of a deer. Unlike the conventional round ganoderma lucidum, the greenish ganoderma lucidum grows naturally in nature, which is said to have an excellent effect of 10 times or more in ingredients. Noggak Territory has been called Bulchocho since ancient times in Korea, and in oriental medicine, it has the effects of tonic, Jinhae, and small sarcoma, so it is effectively used for nervous breakdown, heart disease, high blood pressure, and various cancers. The mushroom stand is wrapped in a hard cuticle and has a glossy varnish.

Baekho Eun-chim (白 대표적인) is the representative tea of white tea produced in Hwa County in northeastern Fujian Province, China. Its appearance is white fluffy (白毫) sent by it, and it is named because it is pointed like saliva. After some fermentation without any special processing, it is dried. So even after long-term storage, there is little change in aroma and taste, the taste is clean and fresh, the sweetness remains, the astringent taste is small, the fragrance is clean, and the tan is pale apricot. The white tiger silver needle is cold because of its nature and good for stomach and gallbladder is rarely used as a herbal medicine.

The cosmetic composition of the present invention is a cosmetic composition comprising fermented extracts of Cheonsam, Noggak ganoderma lucidum and white tiger silver needles obtained by fermenting Cheonsam, Noggak ganoderma lucidum and white tiger silver needles as an active ingredient, and have no skin irritation, and are based on cosmetics. It is a cosmetic composition that is excellent in solubility, affinity, and stability for additives, organic solvents, and the like, and is particularly excellent in increasing antioxidant, collagen synthesis, and inhibition of inflammatory reaction.

The content of the fermented extracts of the Cheonsam, Noggak ganoderma lucidum and white tiger silver needle is preferably included in an amount of 0.01 to 90% by weight based on the total weight of the composition. When the content of the fermented extract contains less than 0.01% by weight does not appear to improve the skin, when the content exceeds 90% by weight the degree of effect increase due to the increase in content is insufficient, there is a problem in the safety and stability of the formulation However, there is a problem that is not economic.

The fermented extracts of Cheonsam, Nocturnal Ganoderma lucidum and white tiger silver needles are preferably fermented with one or more bacteria selected from the group consisting of yeast, lactic acid bacteria and Bacillus genus, and the yeast is preferably Saccharomyces cerevisisae ( Saccharomyces cerevisisae ) More preferably, it is preferable that it is Saccharomyces cerevisisae KCTC7904.

The cosmetic composition may further include components that may be conventionally included in the cosmetic composition, for example, may include one or more selected from the group consisting of antioxidants, stabilizers, solubilizers, vitamins, pigments, perfumes and carriers. However, the present invention is not limited thereto.

The cosmetic composition may be a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, a powder, a soap, a surfactant-containing cleansing, a surfactant-free cleansing, an oil, a powder foundation, an emulsion foundation, a wax foundation or a spray, and the like. It may be formulated as, but is not limited thereto. More specifically, the cosmetic composition is preferably in the form of a flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder, but is not limited thereto. It is not.

When the formulation of the cosmetic composition is a paste, cream or gel, carrier components include animal oil, vegetable oil, wax, paraffin, starch, trakant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. Can be.

When the formulation of the cosmetic composition is a powder or a spray, a carrier component may include lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder, and in the case of a spray, additionally chlorofluorohydrocarbon, propane, It may further include a propellant such as butane or dimethyl ether.

When the formulation of the cosmetic composition is a solution or emulsion, a carrier component may include a solvent, a solubilizer or an emulsifier, and more specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Fatty acid esters of propyleneglycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethyleneglycol or sorbitan.

When the formulation of the cosmetic composition is a suspension, a liquid diluent such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester as a carrier component, micro Crystalline cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like.

When the formulation of the cosmetic composition is a surfactant-containing cleansing, it is an aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyltaurate, sarcosinate, fatty acid amide as a carrier component. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like.

When the formulation of the cosmetic composition is a soap, a surfactant-containing cleansing or a surfactant-free cleansing, after applying to the skin may be wiped off, peeled off or washed with water. As a specific example, the soap is a liquid soap, powdered soap, solid soap or oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel or cleansing pack, the surfactant-free cleansing formulation is a cleansing cream , But not limited to, cleansing lotion, cleansing water or cleansing gel.

The present invention comprises the steps of culturing the ginseng, nocturnal ganoderma and white tiger silver saliva into one or more bacteria selected from the group consisting of yeast, lactic acid bacteria and Bacillus genus; And it provides a method for producing a cosmetic composition comprising the step of extracting the fermentation extract of Cheonsam, Noggak ganoderma lucidum and white tiger.

The Cheonsam, Nocturnal Ganoderma lucidum and white tiger silver needles are selected from the group consisting of yeast, lactic acid bacteria and Bacillus genus including 1 to 60% by weight, 1 to 60% by weight of greenery ganoderma lucidum and 1 to 60% by weight of white tiger silver saliva based on dry weight It is preferable to incubate with one or more bacteria.

The Cheonsam, Nocturnal Ganoderma lucidum and white tiger silver needles are preferably cultivated with one or more bacteria selected from the group consisting of the yeast, lactic acid bacteria and Bacillus genus after the dry grinding, the yeast is Saccharomyces cerevisisae ( Saccharomyces cerevisisae ) And more preferably Saccharomyces cerevisiae KCTC7904 ( Saccharomyces cerevisisae). KCTC7904) is good.

In the step of culturing, the carbon source is preferably 1 to 4% by weight based on the total weight of the strain culture medium, more preferably 1 to 2% by weight. In addition, it is preferable to further include 0.1 to 1% by weight of peptone, based on the total weight of the strain culture medium, more preferably 0.2 to 0.5% by weight.

The culturing step is preferably incubated for 1 to 7 days while maintaining a pH of 5 to 7 at a temperature of 20 to 40 ℃ temperature, but is not limited thereto. When satisfying the above-mentioned culture conditions, the culture of the bacteria is made in the optimum state can be produced a large amount of fermentation extracts of Cheonsam, Noggak ganoderma lucidum and white tiger.

After the fermentation, it is preferable to obtain a fermentation extract by extracting for 1 to 40 hours at 40 to 90 ℃ temperature conditions, more preferably to extract for 12 to 32 hours at 40 to 60 ℃ temperature conditions, most preferably It is recommended to extract for 24 hours at 50 ℃ temperature conditions.

The extraction may be performed using one or more extraction solvents selected from the group consisting of purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, chloroform, ethyl ether, butylene glycol and hexane. It is preferable to use ethanol or purified water more preferably, and most preferably 70% ethanol aqueous solution.

The fermented extracts of the extracted Cheonsam, Noggak ganoderma lucidum and white tiger roe needles are preferably extracted under reflux and cooled by adding ethanol to 60-80% (v / v) ethanol aqueous solution, followed by filtration.

After completion of the filtration, it is preferable to transfer to a concentration tank to concentrate under reduced pressure and freeze-dried it. The decompression concentration can be concentrated using a rotary decompression concentrator, wherein the concentration conditions are reduced to 1 to 3 hours while putting the extract in a flask and rotating at 600 to 1200rpm under a pressure of 650mmHg to 750mmHg, 50 to 70 ℃ temperature conditions Although it is preferable to concentrate, it is not limited to this.

The concentrated solution concentrated under reduced pressure was subjected to prefreezing to lower the temperature to -40 ° C. or lower, and maintained at −40 to −50 ° C. for 2 to 4 hours, and then drop the temperature to −80 ° C. or lower through trap freezing, and vacuum to 10 to 30 mTorr. It is preferable to raise again to -40 degreeC or more over 60-180 minutes after hold | maintaining. After that, it is preferable to hold at -20 ° C for 240 minutes, at 0 ° C for 40 minutes, at 15 ° C for 340 minutes, and at 30 ° C for 130 minutes, and for 1300 minutes until it is completely dried, but is not limited thereto.

Furthermore, the cosmetic composition of the present invention preferably comprises an extract obtained after the ultra high pressure treatment of the fermented extracts of Cheonsam, Noggak ganoderma lucidum and white tiger silver needle, the extract is vacuum, concentrated and lyophilized powder or a blend of the powder with an extractant. However, it is not limited thereto.

Hereinafter, the present invention will be described in detail with reference to the following examples. However, the present invention is not limited to the following examples.

< Manufacturing example  1: thousandsam, Nogga , Silver tiger  Fermented Extract Manufacturer>

Cheonsam 30g, Noggak ganoderma lucidum 20g, and 50g white tiger silver needle were selected as a sample, sufficiently washed and dried to make a pulverized material to pass through a 300 mesh sieve. 0.5 g based on solids of the pulverized product Saccharomyces cerevisisae KCTC 7904 strain cultures were added by dissolving to levels.

After the strain culture solution was added, 2% (w / v) of glucose was added as a carbon source, and 0.5% (w / v) of peptone was further added and cultured. The culture was incubated for 5 days while maintaining the pH at 37 ℃, 5L fermenter.

Saccharomyces cerevisiae KCTC 7904 ( Saccharomyces cerevisisae KCTC 7904) After the culture was cultured by centrifugation to remove the culture. Cheongsam, green ganoderma lucidum and white tiger silver needles from which the culture was first removed were heated to 50 ° C., treated with 70% ethanol for 24 hours, and then cooled to room temperature.

Fermented products of Cheonsam, Noggak ganoderma lucidum and white tiger roe treated with 70% ethanol were refluxed three times for 1 hour, cooled, and filtered through Whatman # 2 filter paper.

After filtration was completed, the extract was transferred to a concentration tank, concentrated under reduced pressure at 50 ° C. or lower, and lyophilized. Then, based on the total weight of the fermented extract using 50% glycerin, Cheonsam, Nocturnal Ganoderma lucidum and white tiger silver needle were included as 2% by weight. 20g of fermented extracts of Cheonsam, Noggak ganoderma lucidum and white tiger silver needle were prepared.

< Manufacturing example  2: Preparation of Fermented Extract of Cheonsam>

In Preparation Example 1, 20g of Cheonsam was prepared in the same manner as in Preparation Example 1, except that 100g of Cheonsam was used instead of 30g of Cheonsam, 20g of Nocturnal Ganoderma lucidum and 50g of White Tiger Silver Needle.

< Manufacturing example  3: Greenery  Fermented Extract Manufacturer>

In Preparation Example 1, instead of the sample of 30g Cheonsam 30g, Noggak ganoderma lucidum 20g, and 50g white tiger silver needle, except that a sample of 100g noggak ganoderma lucidum was carried out in the same manner as in Preparation Example 1 to prepare a 20g fermented extract of Noggak ganoderma lucidum. .

< Manufacturing example  4: Silver tiger  Fermented Extract Manufacturer>

In Preparation Example 1, instead of the sample of 30g Cheonsam 30g, Noggak ganoderma lucidum 20g and 50g silver roe needle, 100g of white tiger silver needle was carried out in the same manner as in Preparation Example 1 to prepare 20g of fermented extract of white tiger silver needle. .

< Example  1 to 3 and Comparative Example  1 to 4: Preparation of Nutritional Cream>

According to the composition described in Table 1 below, a) heating the dissolved portion of purified water to 80 ℃ dissolution; b) emulsifying by adding the oil phase portion heated and dissolved at 80 ° C .; c) adding and mixing the thickener dissolved by heating at 60 ° C; d) adding and mixing cosmetic ingredients after cooling; e) defoaming and filtering after cooling to 30 ° C .; F) through the step of aging at 50 ℃, the fermented extracts of Cheonsam, Noggak ganoderma lucidum, white tiger silver needles prepared in Preparation Example 1 to 1, 5 and 10% by weight relative to the total weight of the composition to add a concentration of 10, At the level of 50, 100 µg / ml The nutritive creams of Examples 1 to 3 were prepared. Nourishing cream of Comparative Example 1 was prepared without the addition of fermented extracts of Cheonsam, Noggak ganoderma and baekhoeun needles. A nutritive cream of 4 to 4 was prepared.

Classification Raw material name Example 1 (% by weight) Example 2 (% by weight) Example 3 (% by weight) Comparative Example 1 (% by weight) Comparative Example 2 (% by weight) Comparative Example 3 (wt% Comparative Example 4 (wt%) Purified water part Purified water 61.36 57.36 52.36 62.36 61.36 61.36 61.36 Propylene glycol 5.0 5.0 5.0 5.0 5.0 5.0 5.0 glycerin 6.0 6.0 6.0 6.0 6.0 6.0 6.0 Xanthan gum 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Triethanolamine 0.7 0.7 0.7 0.7 0.7 0.7 0.7 Methylparaben 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Phenoxyethanol 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Fermented Extracts of Cheonsam, Noggak Ganoderma lucidum, and White Tiger 1.0 5.0 10.0 - - - - Fermented Extract of Cheonsam - - - - 1.0 - - Fermented Extract of Nocturnal Ganoderma Lucidum - - - - - 1.0 - Fermented Extract of White Tiger - - - - - - 1.0 Oil component Squalane 8.0 8.0 8.0 8.0 8.0 8.0 8.0 Cetyl alcohol 2.2 2.2 2.2 2.2 2.2 2.2 2.2 Cyclopentasiloxane 2.0 2.0 2.0 2.0 2.0 2.0 2.0 Stearic acid 1.5 1.5 1.5 1.5 1.5 1.5 1.5 Glyceryl monostearate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Microcrystalline Wax 0.6 0.6 0.6 0.6 0.6 0.6 0.6 Fiji-100 0.3 0.3 0.3 0.3 0.3 0.3 0.3 Tocopherol Acetate 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Shea butter 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Propylparaben 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Oryzanol 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Bisabolol 0.2 0.2 0.2 0.2 0.2 0.2 0.2 Dimethicone 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Monostearic acid polyoxyethylene sorbitan 0.8 0.8 0.8 0.8 0.8 0.8 0.8 Thickener Carbomer 0.24 0.24 0.24 0.24 0.24 0.24 0.24 Purified water 2.4 2.4 2.4 2.4 2.4 2.4 2.4 Beauty ingredients Sodium ascorbate 0.1 0.1 0.1 0.1 0.1 0.1 0.1 Phospholipids 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Placenta Extract 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Aloe extract 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Tocopherol acetate 0.5 0.5 0.5 0.5 0.5 0.5 0.5 Aloe extract 1.0 1.0 1.0 1.0 1.0 1.0 1.0 Sum 100.00 100.00 100.00 100.00 100.00 100.00 100.00

< Experimental Example  One: Phototoxicity  And skin protection effect>

10 mg / cm ^{2 of} the nourishing cream prepared in Example 1 above in the forearm portion of 30 healthy adult men and women (15 males, 15 females) After each application, the area not covered with the nourishing cream was covered with aluminum foil, and after 30 minutes, a long wavelength ultraviolet ray was irradiated at a dose of 10.2 J / cm ² using an ultraviolet irradiator and skin condition (erythema, edema, Papules, etc.) were observed.

As a result, no side effects such as erythema, edema, papules, etc. were generated in the skin, and it was confirmed that the nutrient cream had no phototoxicity and had a skin protective effect.

< Experimental Example  2: skin elasticity personality effect>

Experimental group in which 60 healthy males and females (30 males, 30 females) were divided into two groups and the nutrition creams prepared in Example 1 and Comparative Examples 1 to 4 were applied in a fixed amount (10 mg / 5 cm ² ), respectively. 1) and the control group (Comparative Examples 1 to 4), skin elasticity after 1 hour, 3 hours, 6 hours was measured by the hollow cylindrical shape consisting of a 10 mm diameter frame with a force of about 5 N, allowing time for marks to disappear. The improvement effect was compared.

As a result of the experiment, compared to the control group (Comparative Examples 1 to 4) at about 12 to 20% level at each time, the experimental group (Example 1) is seen as disappearing from the fermented extract of Cheonsam, Nocturnal ganoderma lucidum and white tiger silver needle of the present invention. It was confirmed that the skin elasticity is improved by the use of the nourishing cream.

Example 1
(time) Comparative Example 1
(time) Comparative Example 2
(time) Comparative Example 3
(time) Comparative Example 4
(time) 1 hours 0.42 0.51 0.47 0.48 0.48 3 hours 0.53 0.63 0.64 0.63 0.67 6 hours 0.58 0.69 0.70 0.69 0.72

< Experimental Example  3: skin stability effect>

A human patch test was carried out on the forearm of 30 healthy adult men and women (15 males, 15 females) with the nutrition cream prepared in Example 1 and Comparative Examples 1-4. After wiping the forearm of the test subject well with 70% ethanol, the pin chamber containing 20 µl of the nourishing cream prepared in Example 1 in a conventional cosmetic base was closed and attached for 24 hours using an adhesive tape. After 24 hours, the adhesive tape and the pin chamber were removed, the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours.

As a result of the experiment, as shown in Table 3, in the human patch test to confirm the skin irritation of the nourishing cream containing fermented extracts of Cheonsam, Noggak ganoderma lucidum and Baekhoeun saliva according to the present invention did not show a positive response to the human skin It can be seen that there is no.

After 24 hours (n = 30)
Number of reaction quantums / total number of targets After 48 hours (n = 30)
Number of reaction quantums / total number of targets Example 1 0 0 Comparative Example 1 One 0 Comparative Example 2 0 One Comparative Example 3 0 2 Comparative Example 4 One 0

< Experimental Example  4: antioxidant effect

The amount of free radicals formed in the cells was measured to evaluate the antioxidant effect on the nutritive creams prepared in Examples 1 to 3 and Comparative Example 1. The antioxidant effect was determined by fluorimetric assay using 2 ', 7'-dichlorofluorescin diacetate (DCFH-DA), DCFH-DA non-fluorescent in cells When separated by the polar molecule DCFH and oxidized by free radicals in the cell is converted to fluorescent 2 ', 7'-dichlorofluorescin (2', 7'-dichlorofluorescin, DCF), the 2 ', 7'-dichloro The intensity of fluorescein (2 ', 7'-dichlorofluorescin, DCF) was measured by a fluorometer. As a result of measuring the intensity of 2 ', 7'-dichlorofluorescein (2', 7'-dichlorofluorescin, DCF), as shown in Table 4 and FIG. 1, 2 ', 7'-dichloro for Examples 1 to 3 The intensity of fluorescein (2 ', 7'-dichlorofluorescin, DCF) is reduced, it can be seen that the amount of free radicals in the cell is reduced, through which the nutritional cream according to the present invention has excellent antioxidant effect have.

Example 1 Example 2 Example 3 Comparative Example 1 DCF strength 87% 82% 76% 100%

< Experimental Example  5: inhibitory effect of inflammatory response>

The inhibitory effect of intracellular inflammation on the nutritional creams of Examples 1 to 3 and Comparative Example 1 was evaluated through the amount of beta-hexosaminidase.

As a result of measuring the release amount of beta-hexosaminidase (β-hexosaminidase), as shown in Table 5 and FIG. 2, the release amount of beta-hexosaminidase was reduced in Examples 1 to 3 and inflammation It was found to be effective in suppressing the reaction.

Example 1 Example 2 Example 3 Comparative Example 1 Release of Beta-Hexosaminidase 89% 92% 83% 100%

< Experimental Example  6: increase collagen synthesis>

The effect of increasing collagen synthesis on the nutritive creams of Examples 1 to 3 and Comparative Example 1 was evaluated through the concentration of procollagen type I carboxy-terminal peptide (PIP).

As a result of measuring the concentration of the procollagen type I C-terminal peptide (PIP), as shown in Table 6 and FIG. 3, the procollagen type I C-terminal peptide ( The concentration of procollagen type I carboxy-terminal peptide (PⅠP) is increased, indicating that it is effective for intracellular collagen synthesis and wrinkle improvement.

Example 1 Example 2 Example 3 Comparative Example 1 Procollagen Type I C-Term Peptide (PⅠP) Concentration 103% 116% 120% 100%

Claims (7)

Cosmetic composition comprising a fermented extract of Cheonsam, Noggak ganoderma lucidum and baekhoeun saliva as an active ingredient. The cosmetic composition according to claim 1, wherein the content of the fermented extract of Cheonsam, Noggak ganoderma lucidum and white tiger salivary impregnation is 0.01 to 90% by weight based on the total weight of the composition. The cosmetic composition according to claim 1, wherein the fermented extracts of Cheonsam, Noggak ganoderma lucidum and baekhoeun needles are fermented with one or more bacteria selected from the group consisting of yeast, lactic acid bacteria and Bacillus genus bacteria. The cosmetic composition according to claim 3, wherein the yeast is Saccharomyces cerevisisae . The cosmetic composition according to claim 1, wherein the cosmetic composition is a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence, an eye cream, a cleansing cream, a cleansing foam, a cleansing water, a pack, a spray, or a powder. Cultivating Cheonsam, Nocturnal Ganoderma lucidum and Baekgoeun Saliva into one or more bacteria selected from the group consisting of yeast, lactic acid bacteria and Bacillus genus; And
Method of producing a cosmetic composition comprising the step of extracting fermented extracts of Cheonsam, Noggak ganoderma lucidum and white tiger roe. The method of claim 6, wherein the culturing is a method for producing a cosmetic composition, characterized in that the culture for 1 to 7 days at pH 5 to 7 at 20 to 40 ℃ temperature conditions.

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