KR101629453B1

KR101629453B1 - Composition containing Mantidid ootheca extract for skin volume augmentation or improving wrinkle and elasticity

Info

Publication number: KR101629453B1
Application number: KR1020150173277A
Authority: KR
Inventors: 윤경섭; 김미진; 정택규; 박현철
Original assignee: 주식회사 사임당화장품
Priority date: 2015-12-07
Filing date: 2015-12-07
Publication date: 2016-06-10

Abstract

The present invention relates to a composition for promoting differentiation from adipose precursor cells into adipocytes. More specifically, it contains an extract of a trademarked herb as an active ingredient to promote the differentiation of lipid precursor cells into adipocytes, thereby increasing the volume of the skin to increase the volume of the skin, promoting collagen and elastin synthesis to improve wrinkles and promote elasticity &Lt; / RTI >

Description

[0001] The present invention relates to a composition for improving skin volume, wrinkles and elasticity,

The present invention relates to a composition capable of increasing the volume of skin and improving wrinkles and elasticity, and relates to a composition which is effective for reducing skin volume and improving wrinkles and elasticity due to aging.

Skin is an organ that covers the outside of the body. It consists of three layers: the epidermis, the dermis, and the hypodermis. The subcutaneous fat layer below the dermis consists of mature adipocytes that are formed by the differentiation of lipid precursor cells and lipid precursors. Adipocytes are formed by fibrous connective tissue (septa) ), And the septum contains blood vessels, lymph vessels, and nerves. The subcutaneous fat layer protects the body by preventing the heat damage and absorbing the external impact. The nutrients are stored in the lipid droplet in the form of triglyceride and used as an energy source when necessary. It also functions as a cosmetic with a scaffold that maintains the volume of the skin.

As the age increases, the epidermis and subcutaneous fat layer become thinner due to internal causes such as hormone secretion and biochemical changes, and the rete ridge pattern at the epidermal-dermal boundary is flattened. The young baby's face is generally round and soft. The resilient skin, the rich subcutaneous fat layer, and the soft nose and ear cartilage make this facial contour. By adolescence, facial bones, nose, and cartilage of the ear grow to almost the size of an adult. There is a personal and ethnic difference, but the change of face starts from the mid-twenties according to age. Initially, fine skin aging is progressing, and these skin changes are mainly caused by external factors such as ultraviolet rays and smoking. In the late 20s and 30s, the forehead, including the eyebrows, begins to descend, causing the eyes to look smaller. From the mid-thirties, the elasticity of the skin of the eyelid drops, and the eye fat is pseudoherniated. In addition, glabellar frown lines and nasolabial folds start to become noticeable. In the forties, the forehead wrinkles deepen, crow's-feet, glabel-lar furrows, and eyelids lose their elasticity. Also, irregular lines are formed around the mouth and jaw. In the 50s, the wrinkles around the mouth and neck are clearly visible, the tip of the nose is struck, and the external angle is struck down. The wrinkles on the forehead and eyes are deeper and are observed when the impression is not being used. As the structures in the center of the face are struck, the fat below the eyes falls and the wrinkles deepen. After the age of 60, the skin becomes thinner and a reduction in subcutaneous fat results in deep wrinkles throughout the face (J. Rhinol. 2012, 19 (2), 87-90). In other words, it is known that as the age increases, the lipid precursor cells decrease in differentiation into adipocytes, thereby decreasing the lipogenesis ability of subcutaneous fat and decreasing the volume of skin.

Recently, autologous fat transplantation has been widely used to improve the loss of skin volume associated with the reduction of subcutaneous fat due to aging. Autologous fat transplantation is a method of collecting fat from the thighs or abdomen of a patient and injecting it for the purpose of shaping into the forehead, jaw, ball and nose with reduced skin volume after pretreatment, And it is used to increase skin volume.

However, after fat transplantation, 40-60% of the transplantation site is resorbed in the human body and the long-term effect can not be achieved, and further treatment is required for the continuous effect. In addition, there are problems such as chronic edema at the graft site, acne, sensory disturbance, etc., as well as a problem that the adipose cell mass is touched after fat transplantation at sites where the skin is thin and the subcutaneous fat is small. Accordingly, there is a growing demand for a composition capable of sufficiently increasing and maintaining the subcutaneous fat layer while replacing the fat grafting surgery with these problems.

Trademark Seconds (Mantidis Ootheca, Paratenodera sinensis De Saussure) is also known as a medicinal insects used in herbal remedies and dangrang party (幢螂子), dangrang cattle, sikwoo, sangcho, pyocho or danpyo seconds mantidae warts (Tenodera angustipennis Saussure), some warts (Statilia maculata (Thunberg)), a large ship warts (Hierodula patellifera (Serville), (Mantis and Mantidae)). The warts are cylindrical or semicircular with thin layers of several layers piled up. They are 2.5 to 4.5 cm in length, 2 to 3 cm in width, and 15 to 20 mm in thickness. The outer surface is light brown or yellowish brown, the upper surface is slightly raised, and the lower surface is flat or has a mark attached to the stem of the plant. The outer layer of the cross section represents the sea surface and the inner layer has a small room with several radial arrangements. Each room has a small elliptical egg with dark brown color and one glossy egg. The body is light and the vagina is sick and tired. The nature is sweet and woven, plain and venomous. The wart of this wart is a stratified form, one end is relatively thin, 2.5-5 cm long, and 1-1.5 cm wide. The outer surface is yellowish yellow and the upper surface is banded. Each side of the band has one line of dark green shallow groove and oblique wrinkles. The body is hard and crumbly. This is a parallel rectangle with a length of 2 to 4 cm and a width of 1.5 to 2 cm. The outer surface is grayish brown, the upper side has a banded bump clearly, an oblique wrinkle on both sides, and an upward wing near the tip of the tail. The body is hard and chewy. It has been known to have a long-lasting effect in the one-handed operation, and has a fixed endoscopic efficacy. It has also been used for treatment of eruption opacities, red blood cells, urinary incontinence, and pediatric relief.

Korean Patent Laid-Open Publication No. 2003-0052690 discloses a cosmetic composition for accelerating hair growth, which comprises a trademark herb extract, Korean Patent Laid-Open Publication No. 2007-0109261 discloses a preventive and therapeutic agent for a cardiovascular system disease containing an active ingredient , Korean Patent Laid-Open Publication No. 2000-0000902 discloses a method for producing a thrombolytic component from the brand name.

However, no attempt has been made to increase the skin volume or to determine its efficacy in relation to wrinkle and elasticity improvement using brand name candles.

Korean Patent Publication No. 2003-0052690 Korean Patent Publication No. 2007-0109261 Korean Patent Publication No. 2000-0000902

Accordingly, a problem to be solved by the present invention is to develop a component which can basically increase the volume feeling of skin by promoting differentiation of adipocytes constituting adipose tissue.

More particularly, the present invention relates to a composition capable of increasing the volume of the skin and improving the wrinkles and elasticity of the skin, and a method for producing the same, and is useful as a cosmetic composition for reducing skin volume, Improvement, prevention and treatment, etc.

In order to solve the above-mentioned problems, the present invention provides a composition comprising an extract of Brassica officinalis as an active ingredient.

In one aspect, the present invention provides a composition for external application for skin comprising as an active ingredient a brand-name primrose extract.

In another aspect, the present invention provides a composition for promoting differentiation from adipocyte precursor cells to adipocytes in vitro ( ex vivo ) or in vitro conditions comprising a trademark prima extract as an active ingredient. In addition, the present invention provides a method for differentiating lipoatubular cells into adipocytes in vitro ( ex vivo ) or in vitro ( in vitro ) conditions comprising an extract of Brassica juncea as an active ingredient.

More specifically, the composition according to the present invention acts to promote differentiation into adipocytes in adipose precursor cells. Differentiation accelerating activity of adipocytes in adipose precursor cells of a composition according to the invention can occur in vivo (in vivo), in vitro (ex vivo) or in vitro (in vitro) conditions. In addition, the composition for external application for skin according to the present invention has an action of promoting collagen synthesis in fibroblasts and / or promoting elastin production in fibroblasts. Through these actions, the skin composition according to the present invention can protect the skin and can be used as an external preparation for skin.

Accordingly, the composition for external application for skin according to the present invention may have a skin protection, especially skin volume enhancement, skin elasticity and wrinkle improvement.

In addition, the composition for external application for skin according to the present invention can be used as a cosmetic composition, a pharmaceutical composition, a health food composition and the like.

The inventors of the present invention have conducted researches on various herbal medicines for improving the volume of the skin and improving the wrinkles and elasticity of the skin. As a result, the inventors of the present invention have found that the brand- But the skin elasticity and wrinkle improving effect were found, and the present invention was completed.

Accordingly, in one aspect, the present invention provides a composition for external application for skin volume and elasticity enhancement and wrinkle improvement, which comprises an extract of Brassica officinalis as an active ingredient.

In the present invention, the term skin refers to an organ covering the outside of a human body, and the human body includes a face or a body.

The composition according to the present invention promotes the differentiation of adipocytes. By promoting the differentiation promoting action of adipocytes in vivo, it is possible to improve skin volume and also to improve skin elasticity and wrinkles.

The composition according to the present invention can promote skin collagen production, inhibit collagen degradation, promote elastin production and inhibit elastin degradation, thereby improving skin volume and improving skin elasticity and wrinkles.

In the present invention, the term " differentiation of adipocytes " means the differentiation of an animal, preferably a human preadipocyte or mesenchymal stem cell into adipocytes. Preferably, the lipid precursor cells are differentiated into adipocytes.

In the present invention, the phrase 'enhancing (increasing) skin volume' means increasing the volume of skin adipose tissue, thereby maintaining, improving or improving the firmness or elasticity of the skin, It is possible to prevent or prevent the disappearance of the skin, and to prevent, inhibit or improve the skin aging phenomenon such as wrinkle formation due to loss of skin elasticity.

When the composition for skin external application according to the present invention is applied to a region where improvement of volume, improvement in elasticity and wrinkle is required, volume is improved, elasticity and wrinkles are improved. The area where volume or elasticity is required to be enhanced may be a depressed area due to aging or the like. For example, it may be a depression caused by aging. When the composition for external application for skin according to the present invention is applied to a depressed ball, the volume or elasticity can be improved by promoting differentiation of adipocytes. As another example of a region where volume or elasticity is required to be enhanced, a chest and a buttock can be mentioned. When the composition according to the present invention is applied to the chest and the buttocks, there is an effect that the chest and the buttocks are bulky. Areas where improvement of volume or elasticity and improvement of wrinkles are required include not only visible areas where volume or elasticity reduction or wrinkle generation are visible but also visible areas, have.

Further, the composition according to the present invention can improve the face or body line. Promoting the volume or elasticity of the face and body, promoting wrinkles, promoting biosynthesis of collagen and / or elastin, inhibiting collagen and / or elastin degradation, promoting differentiation of adipocytes, Improved line of damaged face and body can be improved.

The trademark supernatant according to the present invention may include any substance derived from the trademark supernatant, irrespective of the method of extraction and the kind of the constituent. It is preferably selected from the group consisting of water, alcohols having 1 to 4 carbon atoms, acetone, ether, benzene, chloroform, ethyl acetate, dichloromethane, n-hexane, hydrochloric acid, acetic acid, formic acid, citric acid, diethyl ether and cyclohexane May be obtained by solvent extraction with the addition of one or more extraction solvents. Preferably, water and an alcohol having 1 to 4 carbon atoms are added to the extraction solvent. Most preferably, the extraction solvent may be ethanol. The alcohol having 1 to 4 carbon atoms may be 10 to 90% (v / v) alcohol, preferably 50 to 80% (v / v) alcohol, more preferably 70 to 80% v) alcohol.

The composition according to the present invention may contain 0.0001 to 20% by weight, preferably 0.001 to 15% by weight, more preferably 0.01 to 10% by weight, . If the content is less than 0.0001% by weight, no remarkable effect can be expected. If the content is more than 20% by weight, the effect of the extract may not be increased.

The product prepared from the composition for external application for skin containing the active ingredient of the present invention can be produced in the form of a general emulsified formulation and a solubilized formulation. The products of the emulsified formulations include nutritional lotion, cream, and essence, and the product of the solubilized formulation has a softening longevity. It may also be formulated as a topical or systemic adjuvant that is conventionally used in the field of dermatology by containing a dermatologically acceptable medium or base.

The composition for external application for skin according to the present invention may be, for example, a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in an aqueous phase, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) Foam, foam, cleansing foam, cleansing water, lotion, lotion, nutritional lotion, essence, oil, cream, nutrition cream, massage cream, eye cream, cleansing foam, conceal stick, foam A cream, a powder, a pack, a powder, a foundation, a makeup base, a stick, or an external ointment. However, the composition can be formulated into a conventional transdermal preparation.

In addition, the composition for external application for skin according to the present invention may further include one or more dermatologically acceptable carriers on the basis of the active ingredient and those known in the art, and the dermatologically acceptable carriers include, for example, A surfactant, a humectant, a thickening agent, an antioxidant, a viscosity stabilizer, a chelating agent, a buffer, a preservative, a lower alcohol, etc. But the present invention is not limited thereto and the types and concentrations thereof may be varied and may be modified and changed by those skilled in the art without departing from the spirit and scope of the present invention. If necessary, it may contain a whitening agent, a moisturizing agent, an anti-inflammatory agent, an antibacterial agent, an antifungal agent, a vitamin, an ultraviolet screening agent, an antibiotic, an anti-acne agent, a perfume and a dye. May be included in the composition. It is preferably 0.001 to 50% by weight based on the total weight of the composition.

More specifically, as the oil, hydrogenated vegetable oil, pharmacopoeia, cottonseed oil, olive oil, palm oil, jojoba oil and avocado oil may be used. Examples of the wax include wax, wax, carnauba, candelilla, montan, ceresin, Liquid paraffin, lanolin may be used. As the fatty acid, stearic acid, linoleic acid, linolenic acid and oleic acid may be used. As the fatty acid alcohol, cetyl alcohol, octyldodecanol, oleyl alcohol, panthenol, lanolin alcohol, stearyl alcohol and hexadecanol may be used , Isopropyl myristate, isopropyl palmitate, butyl stearate and the like can be used as the fatty acid ester, but the present invention is not limited thereto. Examples of the surfactant include anionic surfactants such as sodium stearate, sodium cetyl sulfate, polyoxyethylene lauryl ether phosphate, sodium N-acyl glutamate; Cationic surfactants such as stearyldimethylbenzylammonium chloride and stearyltrimethylammonium chloride; Amphoteric surfactants such as alkylaminoethylglycine hydrochloride and lecithin; Glycerin monostearate, sorbitan monostearate, propylene glycol monostearate, polyoxyethylene oleyl ether, polyethylene glycol monostearate, polyoxyethylene sorbitan monopalmitate, polyoxyethylene coconut fatty acid monoethaolarnide ), Polyoxypropylene glycol, polyoxyethylene castor oil, nonionic surfactants such as polyoxyethylene lanolin, and the like. As the moisture absorbent, glycerin, 1,3-butylene glycol, propylene glycol may be used, and as the lower alcohol, ethanol and isopropanol may be used. Examples of thickeners include sodium alginate, sodium caseinate, gelatin agar, xanthan gum, starch, cellulose ethers (e.g., hydroxyethylcellulose, methylcellulose, carboxymethylcellulose, hydroxypropylmethylcellulose), polyvinylpyrrolidone, But are not limited to, polyvinyl alcohol, polyethylene glycol, and sodium carboxymethylcellulose. As the antioxidant, butylated hydroxytoluene, butylated hydroxyanisole, propyl gallate, citric acid, and ethoxyquin are usable. As chelating agents, disodium edetate, ethanhydroxy diphosphate is used As the buffer, citric acid, sodium citrate, boric acid, borax, disodium hydrogen phosphate can be used. As the preservative, methyl parahydroxybenzoate, ethyl parahydroxybenzoate, dihydroacetic acid , Salicylic acid, benzoic acid, but are not limited thereto.

In addition, the dermatologically acceptable carrier included in the composition of the present invention may be modified or changed depending on the formulation.

When the formulations of the present invention are ointments, pastes, creams or gels, for example, animal carriers such as animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, , Zinc oxide, or a mixture thereof may be used.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as the carrier component, Propellants such as fluorohydrocarbons, propane / butane or dimethyl ether.

When the formulation of the present invention is a solution or an emulsion, a solvent, a dissolving agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, 1,3-butyl glycol oil, in particular, cottonseed oil, peanut oil, corn oil, olive oil, castor oil and sesame oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester.

When the formulation of the present invention is a suspension, a carrier such as water, a liquid diluent such as ethanol or propylene glycol, a suspension such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracant, etc. may be used.

A suitable dose of the composition for external application for skin according to the present invention varies depending on various factors such as the condition and the weight of the subject, the degree of disease, the form and the period of time, and can be appropriately selected by a person skilled in the art. Depending on the symptoms, it can be administered once a day or divided into several doses.

The composition containing the trademark primer extract according to the present invention as an active ingredient has an effect of promoting differentiation of adipocytes of the skin, promoting collagen production as well as promoting elastin production.

The composition containing the trademark primer extract according to the present invention as an active ingredient has the effect of improving the volume of the skin and improving the wrinkles and elasticity of the skin.

BRIEF DESCRIPTION OF THE DRAWINGS The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate exemplary embodiments of the invention and, together with the description of the invention, It should not be construed as limited.
FIG. 1 shows data on the degree of differentiation of adipose precursor cells into adipocytes when treated with different concentrations of brand-name primers.
FIG. 2 shows data on the effect of the brand-name primrose extract on the enhancement of collagen synthesis when treated at different concentrations.
FIG. 3 shows data on the effect of the extract of Brassica juncea Extract on the enhancement of elastin production when treated at different concentrations.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples. However, the embodiments and experimental examples according to the present invention can be modified into various other forms, and the scope of the present invention should not be construed as being limited to the above-described embodiments and experiments. The embodiments and experimental examples of the present invention are provided to enable those skilled in the art to more fully understand the present invention.

Material preparation

The herbal medicine used in the examples and comparative examples of the present invention was purchased from Jiyu-Honshu Co., Ltd., and the first grade product was used for each reagent.

Example Produce

50 g of Trademark was placed in an extractor, 1 L of a 75% (v / v) ethanol aqueous solution was added as an extracting solvent, and the mixture was reflux cooled and extracted twice at 90 ° C for 4 hours. The resulting extract was cooled to 25 DEG C and filtered to separate the filtrate and residue. The extracted filtrate was concentrated under reduced pressure at 60 DEG C in a distillation apparatus equipped with a cooling condenser.

The extraction yield of Example 1 is shown in Table 1 below.

division Extraction yield (%) Example 14.33

< Experimental Example 1>

Adipose precursor cell Experiments to promote the differentiation of adipocytes

3T3-L1 cells, preadipocytes, were cultured in DMEM (Dulbecco's Modified Eagle Medium, Hyclone Lab., USA) containing 10% bovine calf serum. At a density of 占 10 ⁴ cells / well at 37 占 폚, 5% CO ₂ incubator for 48 hours. After incubation for 48 hours, the cells were treated with 10 μg / mL insulin, 0.5 mM 3-isobutylmethylxanthine, 0.25 μM dexamethasone, 10% fetal bovine serum (FBS) The DMEM medium supplemented was used to induce differentiation into mature adipocytes. At this time, the extracts of the brand - name primers were dissolved in dimethylsulfoxide (DMSO) and treated by concentration. The control group was treated with DMSO. The induction of differentiation was initiated and after 48 hours the extract samples were also treated again with 10% FBS / DMEM medium containing 10 μg / mL insulin. After 72 hours, the cells were replaced with 10% FBS / DMEM medium containing extract samples. Fat-produced by oil-red O staining, which selectively stains only the lipid component in red at 7 days after the start of differentiation into adipocytes Respectively. Cell culture medium was removed and cells were washed with PBS and fixed with 10% formalin for 1 hour at room temperature. After 1 hour, 10% formalin was removed, washed twice with distilled water, and stained with Oil-red O staining reagent for 1 hour at room temperature. After staining, the dyeing reagent was removed, washed with distilled water, scanned, 100% isopropanol was added to the stained cells, and the dyed Oil-red O was eluted. The absorbance was measured at a wavelength of 540 nm, The degree of accumulation was evaluated. Rosiglitazone was used as a positive control. The results are shown in Table 2 and FIG. 1, and experiments were carried out three times, respectively, and the results were shown as a mean value ± standard error.

Sample concentration (μg / mL) Fat accumulation (%) 0 100 10 104.203 ± 2.048 50 136.415 + - 8.222 100 171.770 ± 8.985 Rosiglitazone (1 [mu] M) 190.534 ± 10.423

As shown in the above Table 2 and FIG. 1, the extract of Brassica japonica promoted the differentiation of adipocyte precursor cells into adipocytes in a concentration-dependent manner and showed a similar effect to that of the positive control group, rosiglitazone.

< Experimental Example 2>

Experiments on promoting collagen synthesis in fibroblasts

In order to test skin wrinkle inhibition and improvement effects of the brand-name extract prepared in the above Examples, the promoting effect of collagen synthesis was measured by culturing fibroblasts.

Inoculated with normal human fibroblasts in a 24-well plate at a density of 1 × 10 ⁵ cells / well and incubated for 24 hours at 37 ℃, CO ₂ incubator in a 5% concentration. The extract samples of the Examples were diluted in DMEM (Dulbecco's Modified Eagle's Medium, HyClone Lab., USA) containing no serum by concentration and cultured for 24 hours. The amount of collagen synthesis was determined by measuring the amount of procollagen type I C-peptide (Type I C-peptide, PICP) using a collagen-type IC peptide EIA kit (Takara, Japan). The results are shown in Table 3 and FIG. 2, and the experiments were carried out three times, respectively, and the results were shown as a mean ± standard error. At this time, the absorbance of the cell culture solution not treated with the sample was set to 100% in the control group.

Sample concentration (μg / mL) Collagen synthesis effect (%) 0 100 10 111.810 + - 8.916 50 114.079 ± 5.993

As shown in Table 3 and FIG. 2, it was confirmed that the extract of Brassica japonica exhibited a good collagen synthesis increase effect in a concentration-dependent manner, thereby preventing and improving wrinkles of the skin.

< Experimental Example 3>

Effect of elastin formation in fibroblasts

In order to evaluate the skin elasticity improvement effect of the brand-name extract prepared in the above-mentioned Examples, the promoting effect of elastin production was measured by culturing the fibroblasts.

Normal human fibroblasts were inoculated on a 12-well plate at a density of 2 × 10 ⁵ cells / well and cultured in a CO ₂ incubator at a concentration of 5% at 37 ° C. for 24 hours. The extract samples of the above- ) In DMEM (Dulbecco's Modified Eagle's Medium, HyClone Lab., USA). The amount of newly synthesized elastin in fibroblasts was measured using a commercially available Fastin ( ^TM ) elastin assay kit. The results are shown in Table 4 and FIG. 3, and the experiments were carried out three times, respectively, and the results were expressed as mean value ± standard error. At this time, the absorbance of the cell culture solution not treated with the sample was set to 100% in the control group.

Sample concentration (μg / mL) Effect of elastin synthesis (%) 0 100 10 134.449 ± 17.686 50 144.810 ± 13.588

According to Table 4 and FIG. 3, it can be seen that the brand extender exhibits an effect of increasing elastin synthesis which is excellent in concentration-dependency, thereby improving skin elasticity.

Formulation Example 1 (lotion) and comparison Formulation Example One

Table 5 shows the constitutional components of the skin lotion for increasing the skin volume and the wrinkle and elasticity improving skin containing the active ingredient according to the above Example. Here, the unit of the component content is% by weight. On the other hand, of the components identified by the respective component numbers in the following Table 5, the components 2 and 3 were first dispersed with stirring in the component 1 (purified water), then the components 4 to 6 were added and the components 8 to 10 were dissolved in the component 7 And component 11 were added in this order and mixed. As a comparative formulation example, the components other than the component 11 and the manufacturing method thereof were manufactured in the same manner.

unit
(weight %) ingredient Formulation Example 1 Comparative Formulation Example 1 One Purified water Balance Balance 2 Carbomer 0.1 0.1 3 Hydroxyethyl chylcellulose 0.1 0.1 4 Butylene glycol 2.0 2.0 5 glycerin 1.0 1.0 6 Fig-1500 0.5 0.5 7 ethanol 8.0 8.0 8 Polyoxyethylene hardened castor oil 0.2 0.2 9 Triethanolamine 0.1 0.1 10 antiseptic a very small amount a very small amount 11 The active ingredient material 2.0 -

Formulation Example 2 (lotion) and comparison Formulation Example 2

Table 6 shows the compositions of the lotion for improving skin volume and wrinkle and elasticity containing the active ingredient according to the above Examples. Here, the unit of the component content is% by weight. On the other hand, among the components identified by the respective component numbers in Table 6, Components 1 to 7 were first dissolved by heating at a temperature of 70 占 폚, then Components 8 to 11 were dissolved and dispersed in Component 12 and emulsified by heating at 70 占 폚 . Thereafter, the emulsified product was neutralized with a component 13, cooled to a temperature of 56 캜, and then added with component 14, followed by stirring and cooling to room temperature.

unit
(weight %) ingredient Formulation Example 2 Comparative Formulation Example 2 One Cetearyl alcohol 1.0 1.0 2 Glyceryl stearate / phage-100 stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyl octanoate 6.0 6.0 6 Squalane 4.0 4.0 7 Apricode kernel oil 4.0 4.0 8 glycerin 5.0 5.0 9 Carbomer 0.1 0.1 10 Xanthan gum 0.03 0.03 11 Preservatives and incense a very small amount a very small amount 12 Purified water Balance Balance 13 Arginine 0.1 0.1 14 The active ingredient material 5.0 -

Formulation Example 3 (cream) and compare Formulation Example 3

Table 7 shows the compositions of the creams for improving skin volume and wrinkle and elasticity containing the active ingredient according to the above Examples. Here, the unit of the component content is% by weight. On the other hand, among the components identified by the respective component numbers in Table 7, Components 1 to 7 were first melted by heating at a temperature of 70 캜, then Components 9 to 12 were dissolved and dispersed in Component 13, . Thereafter, the emulsified product was cooled to a temperature of 56 ° C, and then Component 14 was added to the resulting mixture, followed by cooling to room temperature.

unit
(weight %) ingredient Formulation Example 3 Comparative Formulation Example 3 One Cetearyl alcohol 1.0 1.0 2 Glyceryl stearate / phage-100 stearate 1.0 1.0 3 Polysorbate 60 1.0 1.0 4 Sorbitan sesquioleate 0.3 0.3 5 Cetyl octanoate 6.0 6.0 6 Squalane 8.0 8.0 7 Apricode kernel oil 4.0 4.0 8 Dimethicone 2.0 2.0 9 glycerin 5.0 5.0 10 Magnesium aluminum silicate 0.4 0.4 11 Xanthan gum 0.03 0.03 12 Preservatives and incense a very small amount a very small amount 13 Purified water Balance Balance 14 The active ingredient material 10.0 -

< Experimental Example 4>

Increase skin volume and measure wrinkle effect

Evaluation was made using the skin creams prepared in Formulation Example 3 and Comparative Formulation Example 3 in order to evaluate skin volume increase, wrinkle and elasticity improvement effects of the composition for external application for skin according to the present invention.

Specifically, in order to measure skin volume increase, wrinkle and elasticity improvement effects when the creams of Formulation Example 3 and Comparative Formulation Example 3 were respectively applied to the skin, twenty women between 40 and 55 years of age were examined for 12 weeks The cream (test group) of Formulation Example 3 was used on the left side of the region and the cream (control group) of Comparative Formulation Example 3 was used twice continuously on the right side.

The effect of the cream of Formulation Example 3 relative to the cream of Comparative Formulation Example 3 was evaluated relative to the degree of skin volume increase and the effect of improving wrinkles and elasticity. The evaluation was carried out according to the blotting law standards of excellent (5 points), excellent (4 points), normal (3 points), poor (2 points) and very poor (1 point) Respectively.

number Increase skin volume Wrinkle improvement effect Elasticity improvement effect One 4 4 5 2 5 5 5 3 5 5 5 4 4 4 4 5 4 4 4 6 5 5 4 7 5 4 4 8 5 4 4 9 4 3 5 10 5 5 5 11 4 4 3 12 5 5 5 13 5 5 4 14 5 5 5 15 4 4 4 16 5 5 5 17 5 5 4 18 5 5 4 19 4 3 4 20 3 4 4 Average 4.55 4.40 4.35

As shown in Table 8, the relative skin volume increase and wrinkle and elasticity improvement effects of the composition of Formulation Example 3 versus Comparative Formulation Example 3 were 4.55 points, 4.40 points, and 4.35 points, I could.

Claims

A cosmetic composition for external application for skin for increasing skin volume, improving skin elasticity, or improving skin wrinkles, comprising an extract of Chrysanthemum super extract as an active ingredient.

The cosmetic composition for external application for skin according to claim 1, wherein the composition acts to promote differentiation from adipose precursor cells to adipocytes.

[Claim 2] The cosmetic composition for external application for skin according to claim 1, wherein the extraction solvent of the Brassica juncea Extract is water, an alcohol having 1 to 4 carbon atoms, or a mixture thereof.

The cosmetic composition for external application for skin according to claim 1, wherein the composition comprises 0.0001 to 20% by weight based on the total weight of the brand extract.

A cosmetic composition for promoting differentiation from adipocyte precursor cells to adipocytes in vitro ( ex vivo ) or in vitro ( vascular endothelial growth factor ) containing as an active ingredient a trademark prima extract.