JPWO2020126602A5 - - Google Patents
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- Publication number
- JPWO2020126602A5 JPWO2020126602A5 JP2020572480A JP2020572480A JPWO2020126602A5 JP WO2020126602 A5 JPWO2020126602 A5 JP WO2020126602A5 JP 2020572480 A JP2020572480 A JP 2020572480A JP 2020572480 A JP2020572480 A JP 2020572480A JP WO2020126602 A5 JPWO2020126602 A5 JP WO2020126602A5
- Authority
- JP
- Japan
- Prior art keywords
- strand
- amplification
- site
- nucleotides
- complementary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000003321 amplification Effects 0.000 claims 25
- 238000003199 nucleic acid amplification method Methods 0.000 claims 25
- 238000012163 sequencing technique Methods 0.000 claims 25
- 108091093088 Amplicon Proteins 0.000 claims 21
- 108020004707 nucleic acids Proteins 0.000 claims 21
- 150000007523 nucleic acids Chemical class 0.000 claims 21
- 102000039446 nucleic acids Human genes 0.000 claims 21
- 239000002773 nucleotide Substances 0.000 claims 20
- 125000003729 nucleotide group Chemical group 0.000 claims 20
- 238000000034 method Methods 0.000 claims 19
- 230000000295 complement effect Effects 0.000 claims 17
- 238000003776 cleavage reaction Methods 0.000 claims 14
- 230000007017 scission Effects 0.000 claims 14
- 238000006243 chemical reaction Methods 0.000 claims 11
- 208000035657 Abasia Diseases 0.000 claims 3
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 2
- 108060002716 Exonuclease Proteins 0.000 claims 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 238000006073 displacement reaction Methods 0.000 claims 2
- 102000013165 exonuclease Human genes 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- UBKVUFQGVWHZIR-UHFFFAOYSA-N 8-oxoguanine Chemical compound O=C1NC(N)=NC2=NC(=O)N=C21 UBKVUFQGVWHZIR-UHFFFAOYSA-N 0.000 claims 1
- 108020001738 DNA Glycosylase Proteins 0.000 claims 1
- 102000028381 DNA glycosylase Human genes 0.000 claims 1
- 102000004099 Deoxyribonuclease (Pyrimidine Dimer) Human genes 0.000 claims 1
- 108010082610 Deoxyribonuclease (Pyrimidine Dimer) Proteins 0.000 claims 1
- 102000004533 Endonucleases Human genes 0.000 claims 1
- 108010042407 Endonucleases Proteins 0.000 claims 1
- 102000004317 Lyases Human genes 0.000 claims 1
- 108090000856 Lyases Proteins 0.000 claims 1
- 101710147059 Nicking endonuclease Proteins 0.000 claims 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 1
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 claims 1
- 108010072685 Uracil-DNA Glycosidase Proteins 0.000 claims 1
- 239000003398 denaturant Substances 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 108010055863 gene b exonuclease Proteins 0.000 claims 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 claims 1
- 108010068698 spleen exonuclease Proteins 0.000 claims 1
- 230000001131 transforming effect Effects 0.000 claims 1
- 229940035893 uracil Drugs 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862781273P | 2018-12-18 | 2018-12-18 | |
| US62/781,273 | 2018-12-18 | ||
| PCT/EP2019/084183 WO2020126602A1 (en) | 2018-12-18 | 2019-12-09 | Methods and compositions for paired end sequencing using a single surface primer |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2022513546A JP2022513546A (ja) | 2022-02-09 |
| JPWO2020126602A5 true JPWO2020126602A5 (enExample) | 2022-10-05 |
| JP7587425B2 JP7587425B2 (ja) | 2024-11-20 |
Family
ID=68887016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020572480A Active JP7587425B2 (ja) | 2018-12-18 | 2019-12-09 | 単一表面プライマーを使用したペアエンドシーケンシングのための方法および組成物 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US11634765B2 (enExample) |
| EP (1) | EP3899041A1 (enExample) |
| JP (1) | JP7587425B2 (enExample) |
| KR (1) | KR102872035B1 (enExample) |
| CN (1) | CN112739830A (enExample) |
| AU (1) | AU2019411272B2 (enExample) |
| CA (1) | CA3103633A1 (enExample) |
| SG (1) | SG11202012807YA (enExample) |
| WO (1) | WO2020126602A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7569690B2 (ja) | 2018-12-05 | 2024-10-18 | イルミナ ケンブリッジ リミテッド | ブリッジ増幅によるクラスタ生成のための方法および組成物 |
| US12359193B2 (en) | 2022-03-04 | 2025-07-15 | Element Biosciences, Inc. | Single-stranded splint strands and methods of use |
| US12371743B2 (en) | 2022-03-04 | 2025-07-29 | Element Biosciences, Inc. | Double-stranded splint adaptors and methods of use |
| AU2021338761A1 (en) * | 2020-09-11 | 2023-01-05 | Illumina, Inc | Compositions and methods for detecting an abasic site of a nucleic acid |
| US11535892B1 (en) | 2021-06-17 | 2022-12-27 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| US11859241B2 (en) | 2021-06-17 | 2024-01-02 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| WO2022266470A1 (en) * | 2021-06-17 | 2022-12-22 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| CA3224352A1 (en) * | 2021-06-17 | 2022-12-22 | Element Biosciences, Inc. | Compositions and methods for pairwise sequencing |
| CN114457146B (zh) * | 2022-01-28 | 2024-11-19 | 赛纳生物科技(北京)有限公司 | 一种固相介质表面双端扩增测序的方法 |
| CN118272513A (zh) * | 2022-01-28 | 2024-07-02 | 赛纳生物科技(北京)有限公司 | 一种芯片表面双端扩增测序的方法 |
| CA3223037A1 (en) * | 2022-03-30 | 2023-10-05 | Oliver MILLER | Methods for chemical cleavage of surface-bound polynucleotides |
| IL319170A (en) | 2022-09-12 | 2025-04-01 | Element Biosciences Inc | Double-stranded probe adapters with universal long probe strands and methods of use |
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| ATE364718T1 (de) | 1997-04-01 | 2007-07-15 | Solexa Ltd | Verfahren zur vervielfältigung von nukleinsäure |
| DE19812103A1 (de) | 1998-03-19 | 1999-09-23 | Bernauer Annette | Verfahren zur Synthese von Nucleinsäuremolekülen |
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| ES2550513T3 (es) | 2002-08-23 | 2015-11-10 | Illumina Cambridge Limited | Nucleótidos modificados para secuenciación de polinucleótidos |
| US7595883B1 (en) | 2002-09-16 | 2009-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Biological analysis arrangement and approach therefor |
| DE20214753U1 (de) | 2002-09-24 | 2002-12-12 | Hando Handels Ges. M.B.H., Graz | Pharmazeutische Zusammensetzung zur Behandlung und/oder Prävention einer Lichtdermatose |
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| JP7569690B2 (ja) | 2018-12-05 | 2024-10-18 | イルミナ ケンブリッジ リミテッド | ブリッジ増幅によるクラスタ生成のための方法および組成物 |
-
2019
- 2019-12-09 WO PCT/EP2019/084183 patent/WO2020126602A1/en not_active Ceased
- 2019-12-09 SG SG11202012807YA patent/SG11202012807YA/en unknown
- 2019-12-09 CA CA3103633A patent/CA3103633A1/en active Pending
- 2019-12-09 CN CN201980042671.5A patent/CN112739830A/zh active Pending
- 2019-12-09 EP EP19820701.1A patent/EP3899041A1/en active Pending
- 2019-12-09 AU AU2019411272A patent/AU2019411272B2/en active Active
- 2019-12-09 JP JP2020572480A patent/JP7587425B2/ja active Active
- 2019-12-09 KR KR1020207037096A patent/KR102872035B1/ko active Active
- 2019-12-17 US US16/717,303 patent/US11634765B2/en active Active
-
2022
- 2022-12-27 US US18/089,352 patent/US20230137106A1/en active Pending
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