CN112739830A - 使用单一表面引物进行配对末端测序的方法和组合物 - Google Patents

使用单一表面引物进行配对末端测序的方法和组合物 Download PDF

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Publication number
CN112739830A
CN112739830A CN201980042671.5A CN201980042671A CN112739830A CN 112739830 A CN112739830 A CN 112739830A CN 201980042671 A CN201980042671 A CN 201980042671A CN 112739830 A CN112739830 A CN 112739830A
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amplification
strand
nucleic acid
sequencing
nucleotides
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J.M.鲍特尔
P.加蒂-拉夫兰科尼
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Illumina Cambridge Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201980042671.5A 2018-12-18 2019-12-09 使用单一表面引物进行配对末端测序的方法和组合物 Pending CN112739830A (zh)

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US201862781273P 2018-12-18 2018-12-18
US62/781,273 2018-12-18
PCT/EP2019/084183 WO2020126602A1 (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

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US (2) US11634765B2 (enExample)
EP (1) EP3899041A1 (enExample)
JP (1) JP7587425B2 (enExample)
KR (1) KR102872035B1 (enExample)
CN (1) CN112739830A (enExample)
AU (1) AU2019411272B2 (enExample)
CA (1) CA3103633A1 (enExample)
SG (1) SG11202012807YA (enExample)
WO (1) WO2020126602A1 (enExample)

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KR20210104554A (ko) 2021-08-25
AU2019411272B2 (en) 2025-08-14
JP7587425B2 (ja) 2024-11-20
US20230137106A1 (en) 2023-05-04
US20200190578A1 (en) 2020-06-18
WO2020126602A1 (en) 2020-06-25
EP3899041A1 (en) 2021-10-27
CA3103633A1 (en) 2020-06-25
US11634765B2 (en) 2023-04-25
AU2019411272A1 (en) 2021-01-07
KR102872035B1 (ko) 2025-10-16
JP2022513546A (ja) 2022-02-09
SG11202012807YA (en) 2021-01-28

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