CA3103633A1 - Methods and compositions for paired end sequencing using a single surface primer - Google Patents

Methods and compositions for paired end sequencing using a single surface primer Download PDF

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Publication number
CA3103633A1
CA3103633A1 CA3103633A CA3103633A CA3103633A1 CA 3103633 A1 CA3103633 A1 CA 3103633A1 CA 3103633 A CA3103633 A CA 3103633A CA 3103633 A CA3103633 A CA 3103633A CA 3103633 A1 CA3103633 A1 CA 3103633A1
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CA
Canada
Prior art keywords
strand
amplification
nucleic acid
nucleotides
sequencing
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Pending
Application number
CA3103633A
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English (en)
French (fr)
Inventor
Jonathan Mark Boutell
Pietro GATTI-LAFRANCONI
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Illumina Cambridge Ltd
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Illumina Cambridge Ltd
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Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of CA3103633A1 publication Critical patent/CA3103633A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3103633A 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer Pending CA3103633A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862781273P 2018-12-18 2018-12-18
US62/781,273 2018-12-18
PCT/EP2019/084183 WO2020126602A1 (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Publications (1)

Publication Number Publication Date
CA3103633A1 true CA3103633A1 (en) 2020-06-25

Family

ID=68887016

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3103633A Pending CA3103633A1 (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Country Status (9)

Country Link
US (2) US11634765B2 (enExample)
EP (1) EP3899041A1 (enExample)
JP (1) JP7587425B2 (enExample)
KR (1) KR102872035B1 (enExample)
CN (1) CN112739830A (enExample)
AU (1) AU2019411272B2 (enExample)
CA (1) CA3103633A1 (enExample)
SG (1) SG11202012807YA (enExample)
WO (1) WO2020126602A1 (enExample)

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Also Published As

Publication number Publication date
SG11202012807YA (en) 2021-01-28
EP3899041A1 (en) 2021-10-27
KR20210104554A (ko) 2021-08-25
US20230137106A1 (en) 2023-05-04
AU2019411272A1 (en) 2021-01-07
KR102872035B1 (ko) 2025-10-16
CN112739830A (zh) 2021-04-30
AU2019411272B2 (en) 2025-08-14
JP7587425B2 (ja) 2024-11-20
US20200190578A1 (en) 2020-06-18
US11634765B2 (en) 2023-04-25
WO2020126602A1 (en) 2020-06-25
JP2022513546A (ja) 2022-02-09

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