SG11202012807YA - Methods and compositions for paired end sequencing using a single surface primer - Google Patents

Methods and compositions for paired end sequencing using a single surface primer

Info

Publication number
SG11202012807YA
SG11202012807YA SG11202012807YA SG11202012807YA SG11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA SG 11202012807Y A SG11202012807Y A SG 11202012807YA
Authority
SG
Singapore
Prior art keywords
compositions
methods
single surface
end sequencing
paired end
Prior art date
Application number
SG11202012807YA
Other languages
English (en)
Inventor
Jonathan Mark Boutell
Pietro Gatti-Lafranconi
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Publication of SG11202012807YA publication Critical patent/SG11202012807YA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
SG11202012807YA 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer SG11202012807YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862781273P 2018-12-18 2018-12-18
PCT/EP2019/084183 WO2020126602A1 (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Publications (1)

Publication Number Publication Date
SG11202012807YA true SG11202012807YA (en) 2021-01-28

Family

ID=68887016

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11202012807YA SG11202012807YA (en) 2018-12-18 2019-12-09 Methods and compositions for paired end sequencing using a single surface primer

Country Status (9)

Country Link
US (2) US11634765B2 (enExample)
EP (1) EP3899041A1 (enExample)
JP (1) JP7587425B2 (enExample)
KR (1) KR102872035B1 (enExample)
CN (1) CN112739830A (enExample)
AU (1) AU2019411272B2 (enExample)
CA (1) CA3103633A1 (enExample)
SG (1) SG11202012807YA (enExample)
WO (1) WO2020126602A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3103736A1 (en) 2018-12-05 2020-06-11 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification
US12359193B2 (en) 2022-03-04 2025-07-15 Element Biosciences, Inc. Single-stranded splint strands and methods of use
US12371743B2 (en) 2022-03-04 2025-07-29 Element Biosciences, Inc. Double-stranded splint adaptors and methods of use
KR20230065932A (ko) * 2020-09-11 2023-05-12 일루미나, 인코포레이티드 핵산의 무염기 부위를 검출하기 위한 조성물 및 방법
US11859241B2 (en) 2021-06-17 2024-01-02 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
CA3224352A1 (en) * 2021-06-17 2022-12-22 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
WO2022266470A1 (en) * 2021-06-17 2022-12-22 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
US11427855B1 (en) 2021-06-17 2022-08-30 Element Biosciences, Inc. Compositions and methods for pairwise sequencing
CN118272513A (zh) * 2022-01-28 2024-07-02 赛纳生物科技(北京)有限公司 一种芯片表面双端扩增测序的方法
CN114457146B (zh) * 2022-01-28 2024-11-19 赛纳生物科技(北京)有限公司 一种固相介质表面双端扩增测序的方法
CA3223037A1 (en) * 2022-03-30 2023-10-05 Oliver MILLER Methods for chemical cleavage of surface-bound polynucleotides
CA3267226A1 (en) 2022-09-12 2024-03-21 Element Biosciences, Inc. DOUBLE STRAND TO STRAND SPLINT ADAPTERS, UNIVERSAL LONG SPLINTS AND METHODS OF USE

Family Cites Families (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
CA2044616A1 (en) 1989-10-26 1991-04-27 Roger Y. Tsien Dna sequencing
US5552278A (en) 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
US6327410B1 (en) 1997-03-14 2001-12-04 The Trustees Of Tufts College Target analyte sensors utilizing Microspheres
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
DE19812103A1 (de) 1998-03-19 1999-09-23 Bernauer Annette Verfahren zur Synthese von Nucleinsäuremolekülen
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20050181440A1 (en) 1999-04-20 2005-08-18 Illumina, Inc. Nucleic acid sequencing using microsphere arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
DK2264189T3 (en) 1999-04-20 2014-12-08 Illumina Inc Detection of nucleic acid reactions of bead - arrays
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
DE60136166D1 (de) 2000-02-07 2008-11-27 Illumina Inc Nukleinsäure-nachweisverfahren mit universellem priming
US6770441B2 (en) 2000-02-10 2004-08-03 Illumina, Inc. Array compositions and methods of making same
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
AR031640A1 (es) 2000-12-08 2003-09-24 Applied Research Systems Amplificacion isotermica de acidos nucleicos en un soporte solido
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US7595883B1 (en) 2002-09-16 2009-09-29 The Board Of Trustees Of The Leland Stanford Junior University Biological analysis arrangement and approach therefor
DE20214753U1 (de) 2002-09-24 2002-12-12 Hando Handels Ges. M.B.H., Graz Pharmazeutische Zusammensetzung zur Behandlung und/oder Prävention einer Lichtdermatose
JP2007525963A (ja) 2003-06-20 2007-09-13 イルミナ インコーポレイテッド 全ゲノム増幅および遺伝型決定のための方法および組成物
EP1701785A1 (en) 2004-01-07 2006-09-20 Solexa Ltd. Modified molecular arrays
US20060062531A1 (en) 2004-09-17 2006-03-23 Stephen Turner Fabrication of optical confinements
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
SG169356A1 (en) 2006-02-08 2011-03-30 Illumina Cambridge Ltd Method for sequencing a polynucleotide template
EP2018622B1 (en) 2006-03-31 2018-04-25 Illumina, Inc. Systems for sequence by synthesis analysis
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677309B9 (en) 2006-12-14 2014-11-19 Life Technologies Corporation Methods for sequencing a nucleic acid using large scale FET arrays, configured to measure a limited pH range
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US8198023B2 (en) 2008-08-05 2012-06-12 Pacific Biosciences Of California, Inc. Prevention and alleviation of steric hindrance during single molecule nucleic acid synthesis by a polymerase
US8586310B2 (en) 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US9029103B2 (en) 2010-08-27 2015-05-12 Illumina Cambridge Limited Methods for sequencing polynucleotides
US9096899B2 (en) 2010-10-27 2015-08-04 Illumina, Inc. Microdevices and biosensor cartridges for biological or chemical analysis and systems and methods for the same
CN103370425B (zh) 2010-12-17 2019-03-19 生命技术公司 用于核酸扩增的方法、组合物、系统、仪器和试剂盒
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US8778848B2 (en) 2011-06-09 2014-07-15 Illumina, Inc. Patterned flow-cells useful for nucleic acid analysis
US9139874B2 (en) * 2011-07-07 2015-09-22 Life Technologies Corporation Bi-directional sequencing compositions and methods
ES2639938T5 (es) 2011-09-23 2021-05-07 Illumina Inc Métodos y composiciones para la secuenciación de ácidos nucleicos
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
EP2888371B1 (en) * 2012-08-24 2017-08-02 Life Technologies Corporation Methods, compositions, systems, apparatuses and kits for nucleic acid paired end sequencing
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
DK3201355T3 (en) 2014-09-30 2019-10-14 Illumina Inc Modified polymerases for improved incorporation of nucleotide analogues
EP3218511B1 (en) 2014-11-11 2020-04-22 Illumina Cambridge Limited Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
KR20170135834A (ko) 2015-02-10 2017-12-08 일루미나, 인코포레이티드 세포 성분을 분석하기 위한 방법 및 조성물
ES2873723T3 (es) 2016-07-22 2021-11-03 Univ Oregon Health & Science Colecciones de genoma completo de células individuales y métodos de indexación combinatoria para prepararlas
ES2882608T3 (es) 2017-04-23 2021-12-02 Illumina Inc Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados
ES2929837T3 (es) 2017-04-23 2022-12-02 Illumina Cambridge Ltd Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácidos nucleicos indexados
WO2018197950A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
EP3615671B1 (en) 2017-04-23 2021-07-21 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
CA3103736A1 (en) 2018-12-05 2020-06-11 Illumina Cambridge Limited Methods and compositions for cluster generation by bridge amplification

Also Published As

Publication number Publication date
CN112739830A (zh) 2021-04-30
WO2020126602A1 (en) 2020-06-25
JP2022513546A (ja) 2022-02-09
KR20210104554A (ko) 2021-08-25
KR102872035B1 (ko) 2025-10-16
CA3103633A1 (en) 2020-06-25
EP3899041A1 (en) 2021-10-27
AU2019411272A1 (en) 2021-01-07
US20230137106A1 (en) 2023-05-04
JP7587425B2 (ja) 2024-11-20
AU2019411272B2 (en) 2025-08-14
US11634765B2 (en) 2023-04-25
US20200190578A1 (en) 2020-06-18

Similar Documents

Publication Publication Date Title
SG11202012807YA (en) Methods and compositions for paired end sequencing using a single surface primer
GB2574746B (en) Engineered polymerases for improved sequencing
IL286089A (en) RNA-guided DNA integration using tn7-like transposons
GB2581629B (en) Sequencing for pipe handling
GB202107805D0 (en) Riser tools and methods
SG11202012758WA (en) Primer oligonucleotide for sequencing
HUE062589T2 (hu) Csempeszintezõ
GB2572953B8 (en) Surface Analysis
PL3900573T3 (pl) Urządzenie do zdobienia paznokci
GB201713300D0 (en) Surface modification using abrasive blasting
ZA202101188B (en) Grip assist apparatus with insert
EP3625240A4 (en) SEQUENCING USING NON-NATURAL NUCLEOTIDES
HK40054238A (en) Methods and compositions for paired end sequencing using a single surface primer
SG11202012753SA (en) Sequencing kits
PL3640304T3 (pl) Podkład do farb malarskich i tynków
GB2589212B (en) A nail holding apparatus
HK40080943A (en) Methods and devices using cartridges for sequencing
GB201813171D0 (en) Sequencing method
GB201907101D0 (en) Sequencing method
GB201919362D0 (en) Paint easy
HK40055931A (en) Primer oligonucleotide for sequencing
GB2577060B (en) Adaptor
GB2577325B (en) Releasable clamp
IL266492B (en) Compsite grip/slat-armor
GB2562793B (en) Improved movement joint for between tiles and coatings