JPWO2020006049A5 - - Google Patents
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- JPWO2020006049A5 JPWO2020006049A5 JP2020572532A JP2020572532A JPWO2020006049A5 JP WO2020006049 A5 JPWO2020006049 A5 JP WO2020006049A5 JP 2020572532 A JP2020572532 A JP 2020572532A JP 2020572532 A JP2020572532 A JP 2020572532A JP WO2020006049 A5 JPWO2020006049 A5 JP WO2020006049A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- transposase
- target nucleic
- sample
- crispr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 27
- 150000007523 nucleic acids Chemical group 0.000 claims 22
- 108010020764 Transposases Proteins 0.000 claims 15
- 102000008579 Transposases Human genes 0.000 claims 15
- 108091034117 Oligonucleotide Proteins 0.000 claims 13
- 239000000523 sample Substances 0.000 claims 12
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 11
- 108020004707 nucleic acids Proteins 0.000 claims 11
- 102000039446 nucleic acids Human genes 0.000 claims 11
- 238000001514 detection method Methods 0.000 claims 10
- 101710137500 T7 RNA polymerase Proteins 0.000 claims 9
- 108091033409 CRISPR Proteins 0.000 claims 7
- 238000010354 CRISPR gene editing Methods 0.000 claims 7
- 108010012306 Tn5 transposase Proteins 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 108020004414 DNA Proteins 0.000 claims 3
- 239000012530 fluid Substances 0.000 claims 3
- 238000003780 insertion Methods 0.000 claims 3
- 230000037431 insertion Effects 0.000 claims 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- 241000210552 Carnobacterium gallinarum DSM 4847 Species 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 241000204031 Mycoplasma Species 0.000 claims 2
- 241000878522 Porphyromonas crevioricanis Species 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 239000012472 biological sample Substances 0.000 claims 2
- 210000004369 blood Anatomy 0.000 claims 2
- 239000008280 blood Substances 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 210000002381 plasma Anatomy 0.000 claims 2
- 238000010839 reverse transcription Methods 0.000 claims 2
- 230000035945 sensitivity Effects 0.000 claims 2
- 210000002966 serum Anatomy 0.000 claims 2
- 206010003445 Ascites Diseases 0.000 claims 1
- 241000589941 Azospirillum Species 0.000 claims 1
- 241000606125 Bacteroides Species 0.000 claims 1
- 241001038796 Bacteroides ihuae Species 0.000 claims 1
- 241000605059 Bacteroidetes Species 0.000 claims 1
- 241000605900 Butyrivibrio fibrisolvens Species 0.000 claims 1
- 241000168061 Butyrivibrio proteoclasticus Species 0.000 claims 1
- 241000589876 Campylobacter Species 0.000 claims 1
- 241000909926 Candidatus Methanomethylophilus Species 0.000 claims 1
- 241001040999 Candidatus Methanoplasma termitum Species 0.000 claims 1
- 241000949035 Candidatus Microgenomates Species 0.000 claims 1
- 241001316580 Candidatus Roizmanbacteria Species 0.000 claims 1
- 108700004991 Cas12a Proteins 0.000 claims 1
- 241001643775 Chloroflexus aggregans Species 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 241000974757 Demequina aurantiaca Species 0.000 claims 1
- 241000186394 Eubacterium Species 0.000 claims 1
- 241000178967 Filifactor Species 0.000 claims 1
- 241000589565 Flavobacterium Species 0.000 claims 1
- 241000555689 Flavobacterium branchiophilum Species 0.000 claims 1
- 241000589602 Francisella tularensis Species 0.000 claims 1
- 241000032681 Gluconacetobacter Species 0.000 claims 1
- 241000122047 Helcococcus kunzii Species 0.000 claims 1
- 241000613556 Herbinix hemicellulosilytica Species 0.000 claims 1
- 241001134638 Lachnospira Species 0.000 claims 1
- 241000186660 Lactobacillus Species 0.000 claims 1
- 241000589248 Legionella Species 0.000 claims 1
- 208000007764 Legionnaires' Disease Diseases 0.000 claims 1
- 241001148627 Leptospira inadai Species 0.000 claims 1
- 241001453171 Leptotrichia Species 0.000 claims 1
- 241001055859 Leptotrichia buccalis C-1013-b Species 0.000 claims 1
- 241000029603 Leptotrichia shahii Species 0.000 claims 1
- 241000029590 Leptotrichia wadei Species 0.000 claims 1
- 241000778057 Leptotrichia wadei F0279 Species 0.000 claims 1
- 241000186781 Listeria Species 0.000 claims 1
- 241000371296 Listeria riparia Species 0.000 claims 1
- 241000186807 Listeria seeligeri Species 0.000 claims 1
- 241001496637 Listeria weihenstephanensis FSL R9-0317 Species 0.000 claims 1
- 108020005196 Mitochondrial DNA Proteins 0.000 claims 1
- 241000542065 Moraxella bovoculi Species 0.000 claims 1
- 241000293008 Moraxella caprae Species 0.000 claims 1
- 241000588653 Neisseria Species 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 claims 1
- 241000007215 Paludibacter propionicigenes WB4 Species 0.000 claims 1
- 241001386753 Parvibaculum Species 0.000 claims 1
- 208000002151 Pleural effusion Diseases 0.000 claims 1
- 241000605861 Prevotella Species 0.000 claims 1
- 241001302521 Prevotella albensis Species 0.000 claims 1
- 241001646114 Prevotella brevis Species 0.000 claims 1
- 241001299661 Prevotella bryantii Species 0.000 claims 1
- 241001135219 Prevotella disiens Species 0.000 claims 1
- 206010036790 Productive cough Diseases 0.000 claims 1
- 241000202384 Pseudobutyrivibrio ruminis Species 0.000 claims 1
- 241000730262 Rhodobacter capsulatus DE442 Species 0.000 claims 1
- 241000730265 Rhodobacter capsulatus R121 Species 0.000 claims 1
- 241000433126 Rhodobacter capsulatus SB 1003 Species 0.000 claims 1
- 241000605947 Roseburia Species 0.000 claims 1
- 241000949716 Sphaerochaeta Species 0.000 claims 1
- 241000191940 Staphylococcus Species 0.000 claims 1
- 241000194017 Streptococcus Species 0.000 claims 1
- 241001648293 Succinivibrio dextrinosolvens Species 0.000 claims 1
- 241000123710 Sutterella Species 0.000 claims 1
- 241000206606 Synergistes jonesii Species 0.000 claims 1
- 241000589886 Treponema Species 0.000 claims 1
- 108020005202 Viral DNA Proteins 0.000 claims 1
- 241000274840 [Clostridium] aminophilum DSM 10710 Species 0.000 claims 1
- 241001531273 [Eubacterium] eligens Species 0.000 claims 1
- 241001531188 [Eubacterium] rectale Species 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 210000000941 bile Anatomy 0.000 claims 1
- 210000001124 body fluid Anatomy 0.000 claims 1
- 239000010839 body fluid Substances 0.000 claims 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 claims 1
- 230000007613 environmental effect Effects 0.000 claims 1
- 210000000416 exudates and transudate Anatomy 0.000 claims 1
- 210000003608 fece Anatomy 0.000 claims 1
- 229940118764 francisella tularensis Drugs 0.000 claims 1
- 238000001502 gel electrophoresis Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 claims 1
- 229940039696 lactobacillus Drugs 0.000 claims 1
- 239000000314 lubricant Substances 0.000 claims 1
- 210000002751 lymph Anatomy 0.000 claims 1
- 108010009127 mu transposase Proteins 0.000 claims 1
- 210000004400 mucous membrane Anatomy 0.000 claims 1
- 210000003097 mucus Anatomy 0.000 claims 1
- 239000013612 plasmid Substances 0.000 claims 1
- 239000012521 purified sample Substances 0.000 claims 1
- 238000003753 real-time PCR Methods 0.000 claims 1
- 210000003296 saliva Anatomy 0.000 claims 1
- 241000894007 species Species 0.000 claims 1
- 210000003802 sputum Anatomy 0.000 claims 1
- 208000024794 sputum Diseases 0.000 claims 1
- 238000013518 transcription Methods 0.000 claims 1
- 230000035897 transcription Effects 0.000 claims 1
- 210000002700 urine Anatomy 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862690160P | 2018-06-26 | 2018-06-26 | |
| US62/690,160 | 2018-06-26 | ||
| US201862767077P | 2018-11-14 | 2018-11-14 | |
| US62/767,077 | 2018-11-14 | ||
| PCT/US2019/039195 WO2020006049A1 (en) | 2018-06-26 | 2019-06-26 | Crispr/cas and transposase based amplification compositions, systems and methods |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021528975A JP2021528975A (ja) | 2021-10-28 |
| JPWO2020006049A5 true JPWO2020006049A5 (https=) | 2022-07-01 |
| JP2021528975A5 JP2021528975A5 (https=) | 2022-07-01 |
Family
ID=67263112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020572532A Pending JP2021528975A (ja) | 2018-06-26 | 2019-06-26 | Ｃｒｉｓｐｒ／ｃａｓ及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US12559786B2 (https=) |
| EP (1) | EP3814519A1 (https=) |
| JP (1) | JP2021528975A (https=) |
| KR (1) | KR20210024009A (https=) |
| CN (1) | CN112368396A (https=) |
| AU (1) | AU2019294690A1 (https=) |
| BR (1) | BR112020025319A2 (https=) |
| CA (1) | CA3101066A1 (https=) |
| MX (1) | MX2020013778A (https=) |
| SG (1) | SG11202011240YA (https=) |
| WO (1) | WO2020006049A1 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019010422A1 (en) | 2017-07-07 | 2019-01-10 | The Broad Institute, Inc. | ANTIVIRAL THERAPY BASED ON THE CRISPR SYSTEM |
| WO2019094955A1 (en) | 2017-11-13 | 2019-05-16 | The Broad Institute, Inc. | Methods and compositions for targeting developmental and oncogenic programs in h3k27m gliomas |
| SG11202011240YA (en) | 2018-06-26 | 2020-12-30 | Broad Inst Inc | Crispr/cas and transposase based amplification compositions, systems and methods |
| US12402610B2 (en) | 2018-11-09 | 2025-09-02 | The Broad Institute, Inc. | Methods and compositions for modulating innate lymphoid cell pathogenic effectors |
| JP7831937B2 (ja) | 2019-09-03 | 2026-03-17 | クリエイト・メディシンズ，インコーポレーテッド | ゲノム組込みのための方法および組成物 |
| WO2021146641A1 (en) | 2020-01-17 | 2021-07-22 | The Broad Institute, Inc. | Small type ii-d cas proteins and methods of use thereof |
| CA3210883A1 (en) * | 2021-02-09 | 2022-08-18 | Sherlock Biosciences, Inc. | Nucleic acid amplification using promoter primers |
| AU2022272235A1 (en) | 2021-05-11 | 2023-12-21 | Create Medicines, Inc. | Methods and compositions for genomic integration |
| JP2024524773A (ja) | 2021-07-08 | 2024-07-05 | モンタナ ステート ユニバーシティ | Ｃｒｉｓｐｒを用いたプログラム可能なｒｎａ編集 |
| US11814689B2 (en) | 2021-07-21 | 2023-11-14 | Montana State University | Nucleic acid detection using type III CRISPR complex |
| KR102576803B1 (ko) * | 2022-10-14 | 2023-09-12 | 주식회사 풀무원 | CRISPR-Cas 기반의 리스테리아 모노사이토제네스 검출용 조성물 및 이를 이용한 리스테리아 모노사이토제네스 검출방법 |
| US12435357B2 (en) | 2022-12-22 | 2025-10-07 | Kanso Diagnostics Ltd. | Diagnostic device for detecting a target nucleic acid molecule in a biological sample |
| WO2024238563A2 (en) * | 2023-05-15 | 2024-11-21 | Vedabio, Inc. | Arrayed detection of target molecules via crispr cascade reactions |
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| EP2308978B8 (en) | 2003-10-10 | 2015-04-08 | Promega Corporation | Luciferase biosensor |
| US9193959B2 (en) | 2010-04-16 | 2015-11-24 | Roche Diagnostics Operations, Inc. | T7 RNA polymerase variants with enhanced thermostability |
| KR102530118B1 (ko) | 2012-07-25 | 2023-05-08 | 더 브로드 인스티튜트, 인코퍼레이티드 | 유도 ｄｎａ 결합 단백질 및 게놈 교란 도구 및 이의 적용 |
| CN105121648B (zh) | 2012-12-12 | 2021-05-07 | 布罗德研究所有限公司 | 用于序列操纵的系统、方法和优化的指导组合物的工程化 |
| WO2014093709A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| DK2931898T3 (en) | 2012-12-12 | 2016-06-20 | Massachusetts Inst Technology | CONSTRUCTION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION WITH FUNCTIONAL DOMAINS |
| WO2014093718A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
| AU2013359262C1 (en) | 2012-12-12 | 2021-05-13 | Massachusetts Institute Of Technology | CRISPR-Cas component systems, methods and compositions for sequence manipulation |
| WO2014093694A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes |
| CA2894684A1 (en) | 2012-12-12 | 2014-06-19 | The Broad Institute, Inc. | Engineering and optimization of improved crispr-cas systems, methods and enzyme compositions for sequence manipulation in eukaryotes |
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| DK2931897T3 (en) | 2012-12-12 | 2018-02-05 | Broad Inst Inc | CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS |
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| KR20160019553A (ko) | 2013-06-17 | 2016-02-19 | 더 브로드 인스티튜트, 인코퍼레이티드 | 유사분열 후 세포의 질병 및 장애를 표적화하고 모델링하기 위한 시스템, 방법 및 조성물의 전달, 유전자 조작 및 최적화 |
| EP3011030B1 (en) | 2013-06-17 | 2023-11-08 | The Broad Institute, Inc. | Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation |
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| KR20250012194A (ko) | 2013-06-17 | 2025-01-23 | 더 브로드 인스티튜트, 인코퍼레이티드 | 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용 |
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| BR112016013207A2 (pt) | 2013-12-12 | 2017-09-26 | Massachusetts Inst Technology | administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para o hbv e distúrbios e doenças virais |
| AU2014361834B2 (en) | 2013-12-12 | 2020-10-22 | Massachusetts Institute Of Technology | CRISPR-Cas systems and methods for altering expression of gene products, structural information and inducible modular Cas enzymes |
| EP3470089A1 (en) | 2013-12-12 | 2019-04-17 | The Broad Institute Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components |
| JP6793547B2 (ja) | 2013-12-12 | 2020-12-02 | ザ・ブロード・インスティテュート・インコーポレイテッド | 最適化機能ＣＲＩＳＰＲ−Ｃａｓ系による配列操作のための系、方法および組成物 |
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| AU2014361781B2 (en) | 2013-12-12 | 2021-04-01 | Massachusetts Institute Of Technology | Delivery, use and therapeutic applications of the CRISPR -Cas systems and compositions for genome editing |
| CN105463066B (zh) * | 2014-09-04 | 2019-05-17 | 中国科学院北京基因组研究所 | 一种dna扩增方法 |
| EP3889260A1 (en) | 2014-12-12 | 2021-10-06 | The Broad Institute, Inc. | Protected guide rnas (pgrnas) |
| WO2016094872A1 (en) | 2014-12-12 | 2016-06-16 | The Broad Institute Inc. | Dead guides for crispr transcription factors |
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| EP3234187B1 (en) * | 2014-12-15 | 2021-02-17 | Illumina, Inc. | Method for single molecular placement on a substrate |
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| US11155822B2 (en) * | 2015-05-14 | 2021-10-26 | Universidad De Los Andes | Transposon that promotes functional DNA expression in episomal DNAs and method to enhance DNA transcription during functional analysis of metagenomic libraries |
| US10894980B2 (en) * | 2015-07-17 | 2021-01-19 | President And Fellows Of Harvard College | Methods of amplifying nucleic acid sequences mediated by transposase/transposon DNA complexes |
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| CN111108220B (zh) | 2017-03-15 | 2024-11-19 | 博德研究所 | 用于病毒检测的基于crispr效应系统的诊断 |
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| SG11202011240YA (en) | 2018-06-26 | 2020-12-30 | Broad Inst Inc | Crispr/cas and transposase based amplification compositions, systems and methods |
-
2019
- 2019-06-26 SG SG11202011240YA patent/SG11202011240YA/en unknown
- 2019-06-26 AU AU2019294690A patent/AU2019294690A1/en not_active Abandoned
- 2019-06-26 CN CN201980043102.2A patent/CN112368396A/zh active Pending
- 2019-06-26 KR KR1020217001470A patent/KR20210024009A/ko not_active Withdrawn
- 2019-06-26 WO PCT/US2019/039195 patent/WO2020006049A1/en not_active Ceased
- 2019-06-26 EP EP19739830.8A patent/EP3814519A1/en not_active Withdrawn
- 2019-06-26 JP JP2020572532A patent/JP2021528975A/ja active Pending
- 2019-06-26 US US17/054,428 patent/US12559786B2/en active Active
- 2019-06-26 CA CA3101066A patent/CA3101066A1/en active Pending
- 2019-06-26 MX MX2020013778A patent/MX2020013778A/es unknown
- 2019-06-26 BR BR112020025319-7A patent/BR112020025319A2/pt not_active IP Right Cessation
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