JP2021528975A - Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 - Google Patents

Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 Download PDF

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JP2021528975A
JP2021528975A JP2020572532A JP2020572532A JP2021528975A JP 2021528975 A JP2021528975 A JP 2021528975A JP 2020572532 A JP2020572532 A JP 2020572532A JP 2020572532 A JP2020572532 A JP 2020572532A JP 2021528975 A JP2021528975 A JP 2021528975A
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nucleic acid
crispr
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transposase
rna
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フェン チャン
マックス ケルナー
ジョナサン グーテンバーグ
オマール アブデイェー
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Massachusetts Institute of Technology
Broad Institute Inc
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    • C12Y207/00Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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JP2020572532A 2018-06-26 2019-06-26 Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 Pending JP2021528975A (ja)

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US201862690160P 2018-06-26 2018-06-26
US62/690,160 2018-06-26
US201862767077P 2018-11-14 2018-11-14
US62/767,077 2018-11-14
PCT/US2019/039195 WO2020006049A1 (en) 2018-06-26 2019-06-26 Crispr/cas and transposase based amplification compositions, systems and methods

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CN (1) CN112368396A (https=)
AU (1) AU2019294690A1 (https=)
BR (1) BR112020025319A2 (https=)
CA (1) CA3101066A1 (https=)
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SG (1) SG11202011240YA (https=)
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Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12415000B2 (en) 2017-07-07 2025-09-16 The Broad Institute, Inc. CRISPR system based antiviral therapy
WO2019094955A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in h3k27m gliomas
EP3814519A1 (en) 2018-06-26 2021-05-05 The Broad Institute, Inc. Crispr/cas and transposase based amplification compositions, systems and methods
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
CN114981409A (zh) 2019-09-03 2022-08-30 美洛德生物医药公司 用于基因组整合的方法和组合物
WO2021146641A1 (en) 2020-01-17 2021-07-22 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
US20250283152A1 (en) * 2021-02-09 2025-09-11 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
BR112023023642A2 (pt) 2021-05-11 2024-01-30 Myeloid Therapeutics Inc Métodos e composições para integração genômica
WO2023283622A1 (en) 2021-07-08 2023-01-12 Montana State University Crispr-based programmable rna editing
WO2023004391A2 (en) * 2021-07-21 2023-01-26 Montana State University Nucleic acid detection using type iii crispr complex
KR102576803B1 (ko) * 2022-10-14 2023-09-12 주식회사 풀무원 CRISPR-Cas 기반의 리스테리아 모노사이토제네스 검출용 조성물 및 이를 이용한 리스테리아 모노사이토제네스 검출방법
US12435357B2 (en) 2022-12-22 2025-10-07 Kanso Diagnostics Ltd. Diagnostic device for detecting a target nucleic acid molecule in a biological sample
WO2024238563A2 (en) * 2023-05-15 2024-11-21 Vedabio, Inc. Arrayed detection of target molecules via crispr cascade reactions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508014A (ja) * 2003-10-10 2007-04-05 プロメガ コーポレイション ルシフェラーゼバイオセンサー
WO2017015075A1 (en) * 2015-07-17 2017-01-26 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences
WO2018107129A1 (en) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Crispr effector system based diagnostics

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
AU2013293270B2 (en) 2012-07-25 2018-08-16 Massachusetts Institute Of Technology Inducible DNA binding proteins and genome perturbation tools and applications thereof
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
WO2014093655A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
US20140186843A1 (en) 2012-12-12 2014-07-03 Massachusetts Institute Of Technology Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
CN113355357B (zh) 2012-12-12 2024-12-03 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
IL239344B2 (en) 2012-12-12 2024-06-01 Broad Inst Inc Systems engineering, methods and optimal guiding components for sequence manipulation
EP2840140B2 (en) 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
EP3031921B1 (en) 2012-12-12 2025-03-12 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
WO2014093709A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
US11332719B2 (en) 2013-03-15 2022-05-17 The Broad Institute, Inc. Recombinant virus and preparations thereof
EP3725885A1 (en) 2013-06-17 2020-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
MX374532B (es) 2013-06-17 2025-03-06 Broad Inst Inc Suministro, uso y aplicaciones terapéuticas de los sistemas y composiciones crispr-cas, para actuar sobre trastornos y enfermedades utilizando componentes víricos.
WO2014204723A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Oncogenic models based on delivery and use of the crispr-cas systems, vectors and compositions
RU2716420C2 (ru) 2013-06-17 2020-03-11 Те Брод Инститьют Инк. Доставка и применение систем crispr-cas, векторов и композиций для целенаправленного воздействия и терапии в печени
CN105492611A (zh) 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
CN105683379A (zh) 2013-06-17 2016-06-15 布罗德研究所有限公司 用于对有丝分裂后细胞的疾病和障碍进行靶向和建模的系统、方法和组合物的递送、工程化和优化
WO2014204724A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
JP6712948B2 (ja) 2013-12-12 2020-06-24 ザ・ブロード・インスティテュート・インコーポレイテッド 組成物、及びヌクレオチドリピート障害におけるcrispr−cas系の使用方法
WO2015089427A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crispr-cas systems and methods for altering expression of gene products, structural information and inducible modular cas enzymes
EP3470089A1 (en) 2013-12-12 2019-04-17 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
JP6793547B2 (ja) 2013-12-12 2020-12-02 ザ・ブロード・インスティテュート・インコーポレイテッド 最適化機能CRISPR−Cas系による配列操作のための系、方法および組成物
JP7103750B2 (ja) 2013-12-12 2022-07-20 ザ・ブロード・インスティテュート・インコーポレイテッド ゲノム編集のためのCRISPR-Cas系及び組成物の送達、使用及び治療適用
SG10201804975PA (en) 2013-12-12 2018-07-30 Broad Inst Inc Delivery, Use and Therapeutic Applications of the Crispr-Cas Systems and Compositions for HBV and Viral Diseases and Disorders
CN105463066B (zh) * 2014-09-04 2019-05-17 中国科学院北京基因组研究所 一种dna扩增方法
EP3889260A1 (en) 2014-12-12 2021-10-06 The Broad Institute, Inc. Protected guide rnas (pgrnas)
WO2016094872A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Dead guides for crispr transcription factors
WO2016094874A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
DK3234187T3 (da) * 2014-12-15 2021-05-25 Illumina Inc Fremgangsmåde til enkeltmolekyleanbringelse på et substrat
CA2970370A1 (en) 2014-12-24 2016-06-30 Massachusetts Institute Of Technology Crispr having or associated with destabilization domains
US10968536B2 (en) * 2015-02-25 2021-04-06 Jumpcode Genomics, Inc. Methods and compositions for sequencing
WO2016180379A1 (es) * 2015-05-14 2016-11-17 Universidad De Los Andes Nuevo transposón que promueve la expresión funcional de genes en adns episomales y un método para aumentar la transcripción de adn en análisis funcionales de librerías metagenómicas
AU2017257274B2 (en) 2016-04-19 2023-07-13 Massachusetts Institute Of Technology Novel CRISPR enzymes and systems
EP3445853A1 (en) * 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
CN105861552B (zh) * 2016-04-25 2019-10-11 西北农林科技大学 一种T7 RNA聚合酶介导的CRISPR/Cas9基因编辑系统的构建方法
CN105950639A (zh) 2016-05-04 2016-09-21 广州美格生物科技有限公司 金黄色葡萄球菌CRISPR/Cas9系统的制备及其在构建小鼠模型中的应用
CN109642231A (zh) 2016-06-17 2019-04-16 博德研究所 Vi型crispr直向同源物和系统
KR20190140918A (ko) 2017-03-15 2019-12-20 더 브로드 인스티튜트, 인코퍼레이티드 바이러스 검출을 위한 crispr 이펙터 시스템 기반 진단
US10400235B2 (en) * 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN107937328B (zh) * 2017-11-30 2018-09-14 中央民族大学 基于细胞的比较器及应用与细胞计算机
EP3814519A1 (en) 2018-06-26 2021-05-05 The Broad Institute, Inc. Crispr/cas and transposase based amplification compositions, systems and methods

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508014A (ja) * 2003-10-10 2007-04-05 プロメガ コーポレイション ルシフェラーゼバイオセンサー
WO2017015075A1 (en) * 2015-07-17 2017-01-26 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences
WO2018107129A1 (en) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Crispr effector system based diagnostics

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CELL DISCOVERY, vol. 4, no. 20, JPN6023023930, 2018, pages 1 - 4, ISSN: 0005170013 *
SCIENCE, vol. 356, JPN6023023927, 2017, pages 189 - 194, ISSN: 0005082415 *
SCIENCE, vol. 356, JPN6023023928, 2017, pages 438 - 442, ISSN: 0005170011 *
SCIENCE, vol. 360, JPN6023023929, 2018, pages 439 - 444, ISSN: 0005170012 *

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CN112368396A (zh) 2021-02-12
MX2020013778A (es) 2021-05-27
US20210147915A1 (en) 2021-05-20
WO2020006049A1 (en) 2020-01-02
SG11202011240YA (en) 2020-12-30
AU2019294690A1 (en) 2020-12-03
BR112020025319A2 (pt) 2021-03-09
US12559786B2 (en) 2026-02-24
KR20210024009A (ko) 2021-03-04
EP3814519A1 (en) 2021-05-05
CA3101066A1 (en) 2020-01-02

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