JP2021528975A - Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 - Google Patents

Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 Download PDF

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JP2021528975A
JP2021528975A JP2020572532A JP2020572532A JP2021528975A JP 2021528975 A JP2021528975 A JP 2021528975A JP 2020572532 A JP2020572532 A JP 2020572532A JP 2020572532 A JP2020572532 A JP 2020572532A JP 2021528975 A JP2021528975 A JP 2021528975A
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nucleic acid
crispr
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transposase
rna
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フェン チャン
マックス ケルナー
ジョナサン グーテンバーグ
オマール アブデイェー
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Massachusetts Institute of Technology
Broad Institute Inc
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    • C12N2310/00Structure or type of the nucleic acid
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    • C12Y207/07Nucleotidyltransferases (2.7.7)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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JP2020572532A 2018-06-26 2019-06-26 Crispr/cas及びトランスポザーゼを利用した増幅用の組成物、システム、及び方法 Pending JP2021528975A (ja)

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US201862690160P 2018-06-26 2018-06-26
US62/690,160 2018-06-26
US201862767077P 2018-11-14 2018-11-14
US62/767,077 2018-11-14
PCT/US2019/039195 WO2020006049A1 (en) 2018-06-26 2019-06-26 Crispr/cas and transposase based amplification compositions, systems and methods

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CN (1) CN112368396A (https=)
AU (1) AU2019294690A1 (https=)
BR (1) BR112020025319A2 (https=)
CA (1) CA3101066A1 (https=)
MX (1) MX2020013778A (https=)
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Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019010422A1 (en) 2017-07-07 2019-01-10 The Broad Institute, Inc. ANTIVIRAL THERAPY BASED ON THE CRISPR SYSTEM
WO2019094955A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in h3k27m gliomas
SG11202011240YA (en) 2018-06-26 2020-12-30 Broad Inst Inc Crispr/cas and transposase based amplification compositions, systems and methods
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
JP7831937B2 (ja) 2019-09-03 2026-03-17 クリエイト・メディシンズ,インコーポレーテッド ゲノム組込みのための方法および組成物
WO2021146641A1 (en) 2020-01-17 2021-07-22 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
CA3210883A1 (en) * 2021-02-09 2022-08-18 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
AU2022272235A1 (en) 2021-05-11 2023-12-21 Create Medicines, Inc. Methods and compositions for genomic integration
JP2024524773A (ja) 2021-07-08 2024-07-05 モンタナ ステート ユニバーシティ Crisprを用いたプログラム可能なrna編集
US11814689B2 (en) 2021-07-21 2023-11-14 Montana State University Nucleic acid detection using type III CRISPR complex
KR102576803B1 (ko) * 2022-10-14 2023-09-12 주식회사 풀무원 CRISPR-Cas 기반의 리스테리아 모노사이토제네스 검출용 조성물 및 이를 이용한 리스테리아 모노사이토제네스 검출방법
US12435357B2 (en) 2022-12-22 2025-10-07 Kanso Diagnostics Ltd. Diagnostic device for detecting a target nucleic acid molecule in a biological sample
WO2024238563A2 (en) * 2023-05-15 2024-11-21 Vedabio, Inc. Arrayed detection of target molecules via crispr cascade reactions

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508014A (ja) * 2003-10-10 2007-04-05 プロメガ コーポレイション ルシフェラーゼバイオセンサー
WO2017015075A1 (en) * 2015-07-17 2017-01-26 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences
WO2018107129A1 (en) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Crispr effector system based diagnostics

Family Cites Families (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
KR102530118B1 (ko) 2012-07-25 2023-05-08 더 브로드 인스티튜트, 인코퍼레이티드 유도 dna 결합 단백질 및 게놈 교란 도구 및 이의 적용
CN105121648B (zh) 2012-12-12 2021-05-07 布罗德研究所有限公司 用于序列操纵的系统、方法和优化的指导组合物的工程化
WO2014093709A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, models, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
DK2931898T3 (en) 2012-12-12 2016-06-20 Massachusetts Inst Technology CONSTRUCTION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION WITH FUNCTIONAL DOMAINS
WO2014093718A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof
AU2013359262C1 (en) 2012-12-12 2021-05-13 Massachusetts Institute Of Technology CRISPR-Cas component systems, methods and compositions for sequence manipulation
WO2014093694A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
CA2894684A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of improved crispr-cas systems, methods and enzyme compositions for sequence manipulation in eukaryotes
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
DK2931897T3 (en) 2012-12-12 2018-02-05 Broad Inst Inc CONSTRUCTION, MODIFICATION AND OPTIMIZATION OF SYSTEMS, PROCEDURES AND COMPOSITIONS FOR SEQUENCE MANIPULATION AND THERAPEUTICAL APPLICATIONS
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US11332719B2 (en) 2013-03-15 2022-05-17 The Broad Institute, Inc. Recombinant virus and preparations thereof
MX2015017312A (es) 2013-06-17 2017-04-10 Broad Inst Inc Suministro y uso de composiciones, vectores y sistemas crispr-cas para la modificación dirigida y terapia hepáticas.
WO2014204724A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
KR20160019553A (ko) 2013-06-17 2016-02-19 더 브로드 인스티튜트, 인코퍼레이티드 유사분열 후 세포의 질병 및 장애를 표적화하고 모델링하기 위한 시스템, 방법 및 조성물의 전달, 유전자 조작 및 최적화
EP3011030B1 (en) 2013-06-17 2023-11-08 The Broad Institute, Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
WO2014204723A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Oncogenic models based on delivery and use of the crispr-cas systems, vectors and compositions
EP3725885A1 (en) 2013-06-17 2020-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions methods, screens and applications thereof
KR20250012194A (ko) 2013-06-17 2025-01-23 더 브로드 인스티튜트, 인코퍼레이티드 바이러스 구성성분을 사용하여 장애 및 질환을 표적화하기 위한 crispr-cas 시스템 및 조성물의 전달, 용도 및 치료 적용
EP3058091B1 (en) 2013-10-18 2020-03-25 The Broad Institute, Inc. Spatial and cellular mapping of biomolecules in situ by high-throughput sequencing
AU2014346559B2 (en) 2013-11-07 2020-07-09 Editas Medicine,Inc. CRISPR-related methods and compositions with governing gRNAs
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
MX2016007325A (es) 2013-12-12 2017-07-19 Broad Inst Inc Composiciones y metodos de uso de sistemas crispr-cas en desordenes debidos a repeticion de nucleotidos.
BR112016013207A2 (pt) 2013-12-12 2017-09-26 Massachusetts Inst Technology administração, uso e aplicações terapêuticas dos sistemas crispr-cas e composições para o hbv e distúrbios e doenças virais
AU2014361834B2 (en) 2013-12-12 2020-10-22 Massachusetts Institute Of Technology CRISPR-Cas systems and methods for altering expression of gene products, structural information and inducible modular Cas enzymes
EP3470089A1 (en) 2013-12-12 2019-04-17 The Broad Institute Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using particle delivery components
JP6793547B2 (ja) 2013-12-12 2020-12-02 ザ・ブロード・インスティテュート・インコーポレイテッド 最適化機能CRISPR−Cas系による配列操作のための系、方法および組成物
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
AU2014361781B2 (en) 2013-12-12 2021-04-01 Massachusetts Institute Of Technology Delivery, use and therapeutic applications of the CRISPR -Cas systems and compositions for genome editing
CN105463066B (zh) * 2014-09-04 2019-05-17 中国科学院北京基因组研究所 一种dna扩增方法
EP3889260A1 (en) 2014-12-12 2021-10-06 The Broad Institute, Inc. Protected guide rnas (pgrnas)
WO2016094872A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Dead guides for crispr transcription factors
WO2016094874A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Escorted and functionalized guides for crispr-cas systems
EP3234187B1 (en) * 2014-12-15 2021-02-17 Illumina, Inc. Method for single molecular placement on a substrate
WO2016106244A1 (en) 2014-12-24 2016-06-30 The Broad Institute Inc. Crispr having or associated with destabilization domains
WO2016138292A1 (en) * 2015-02-25 2016-09-01 Igenomx International Genomics Corporation Methods and compositions for in silico long read sequencing
US11155822B2 (en) * 2015-05-14 2021-10-26 Universidad De Los Andes Transposon that promotes functional DNA expression in episomal DNAs and method to enhance DNA transcription during functional analysis of metagenomic libraries
EP3445853A1 (en) * 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
US20200263190A1 (en) 2016-04-19 2020-08-20 The Broad Institute, Inc. Novel crispr enzymes and systems
CN105861552B (zh) * 2016-04-25 2019-10-11 西北农林科技大学 一种T7 RNA聚合酶介导的CRISPR/Cas9基因编辑系统的构建方法
CN105950639A (zh) 2016-05-04 2016-09-21 广州美格生物科技有限公司 金黄色葡萄球菌CRISPR/Cas9系统的制备及其在构建小鼠模型中的应用
CA3028158A1 (en) 2016-06-17 2017-12-21 The Broad Institute, Inc. Type vi crispr orthologs and systems
CN111108220B (zh) 2017-03-15 2024-11-19 博德研究所 用于病毒检测的基于crispr效应系统的诊断
US10844372B2 (en) * 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN107937328B (zh) * 2017-11-30 2018-09-14 中央民族大学 基于细胞的比较器及应用与细胞计算机
SG11202011240YA (en) 2018-06-26 2020-12-30 Broad Inst Inc Crispr/cas and transposase based amplification compositions, systems and methods

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508014A (ja) * 2003-10-10 2007-04-05 プロメガ コーポレイション ルシフェラーゼバイオセンサー
WO2017015075A1 (en) * 2015-07-17 2017-01-26 President And Fellows Of Harvard College Methods of amplifying nucleic acid sequences
WO2018107129A1 (en) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Crispr effector system based diagnostics

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CELL DISCOVERY, vol. 4, no. 20, JPN6023023930, 2018, pages 1 - 4, ISSN: 0005170013 *
SCIENCE, vol. 356, JPN6023023927, 2017, pages 189 - 194, ISSN: 0005082415 *
SCIENCE, vol. 356, JPN6023023928, 2017, pages 438 - 442, ISSN: 0005170011 *
SCIENCE, vol. 360, JPN6023023929, 2018, pages 439 - 444, ISSN: 0005170012 *

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SG11202011240YA (en) 2020-12-30
WO2020006049A1 (en) 2020-01-02
MX2020013778A (es) 2021-05-27
CN112368396A (zh) 2021-02-12
CA3101066A1 (en) 2020-01-02
AU2019294690A1 (en) 2020-12-03
US20210147915A1 (en) 2021-05-20
EP3814519A1 (en) 2021-05-05
KR20210024009A (ko) 2021-03-04
BR112020025319A2 (pt) 2021-03-09
US12559786B2 (en) 2026-02-24

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