JPWO2019241535A5 - - Google Patents
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- Publication number
- JPWO2019241535A5 JPWO2019241535A5 JP2020568998A JP2020568998A JPWO2019241535A5 JP WO2019241535 A5 JPWO2019241535 A5 JP WO2019241535A5 JP 2020568998 A JP2020568998 A JP 2020568998A JP 2020568998 A JP2020568998 A JP 2020568998A JP WO2019241535 A5 JPWO2019241535 A5 JP WO2019241535A5
- Authority
- JP
- Japan
- Prior art keywords
- aav
- raav particles
- feed composition
- anion exchange
- exchange chromatography
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862684835P | 2018-06-14 | 2018-06-14 | |
| US62/684,835 | 2018-06-14 | ||
| PCT/US2019/037013 WO2019241535A2 (en) | 2018-06-14 | 2019-06-13 | Anion exchange chromatography for recombinant aav production |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021526829A JP2021526829A (ja) | 2021-10-11 |
| JPWO2019241535A5 true JPWO2019241535A5 (enExample) | 2022-06-20 |
| JP7385603B2 JP7385603B2 (ja) | 2023-11-22 |
Family
ID=67513723
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020568998A Active JP7385603B2 (ja) | 2018-06-14 | 2019-06-13 | 組換えａａｖ生成のためのアニオン交換クロマトグラフィー |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US12070702B2 (enExample) |
| EP (1) | EP3807405A2 (enExample) |
| JP (1) | JP7385603B2 (enExample) |
| KR (1) | KR102930150B1 (enExample) |
| CN (1) | CN112469822A (enExample) |
| AU (1) | AU2019285186B2 (enExample) |
| CA (1) | CA3102817A1 (enExample) |
| IL (2) | IL319002A (enExample) |
| WO (1) | WO2019241535A2 (enExample) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3387118B1 (en) | 2015-12-11 | 2022-04-06 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aavrh10 |
| WO2017100676A1 (en) | 2015-12-11 | 2017-06-15 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav8 |
| US11098286B2 (en) | 2015-12-11 | 2021-08-24 | The Trustees Of The University Of Pennsylvania | Scalable purification method for AAV9 |
| DK3953483T3 (da) | 2019-04-11 | 2023-12-18 | Regenxbio Inc | Fremgangsmåder til størrelseskromatografi til karakterisering af sammensætninger af rekombinant adeno-associeret virus |
| JP7630443B2 (ja) | 2019-04-19 | 2025-02-17 | レジェンクスバイオ インコーポレーテッド | アデノ随伴ウイルスベクター製剤及び方法 |
| WO2021158915A1 (en) * | 2020-02-06 | 2021-08-12 | Precision Biosciences, Inc. | Recombinant adeno-associated virus compositions and methods for producing and using the same |
| EP4192437A4 (en) * | 2020-08-07 | 2024-09-25 | Janssen Biotech, Inc. | FORMULATIONS FOR HIGHLY PURIFIED VIRAL PARTICLES |
| CN116234619A (zh) * | 2020-08-18 | 2023-06-06 | 赛多利斯比亚分离有限责任公司 | 宿主细胞dna的金属亲和提取 |
| AU2021356684A1 (en) | 2020-10-09 | 2023-05-11 | The Trustees Of The University Of Pennsylvania | Compositions and methods for treatment of fabry disease |
| US20230383313A1 (en) | 2020-10-18 | 2023-11-30 | The Trustees Of The University Of Pennsylvania | Improved adeno-associated virus (aav) vector and uses therefor |
| CN117042787A (zh) | 2020-10-29 | 2023-11-10 | 宾夕法尼亚州大学信托人 | Aav衣壳和含有aav衣壳的组合物 |
| EP4240861A1 (en) * | 2020-11-03 | 2023-09-13 | Pfizer Inc. | Methods for purification of aav vectors by anion exchange chromatography |
| KR20230120128A (ko) | 2020-12-16 | 2023-08-16 | 리젠엑스바이오 인크. | 재조합 바이러스 입자의 생산 방법 |
| CA3205209A1 (en) | 2021-01-21 | 2022-07-28 | Regenxbio Inc. | Improved production of recombinant polypeptides and viruses |
| WO2022159679A2 (en) * | 2021-01-21 | 2022-07-28 | Asklepios Biopharmaceutical, Inc. | Method for purifying recombinant viral particles |
| JP7683230B2 (ja) * | 2021-01-27 | 2025-05-27 | 東ソー株式会社 | アデノ随伴ウイルスの精製方法 |
| WO2022191168A1 (ja) * | 2021-03-09 | 2022-09-15 | Ｊｃｒファーマ株式会社 | 組換えａａｖ９ビリオンの製造方法 |
| WO2023060113A1 (en) | 2021-10-05 | 2023-04-13 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| CN118202060A (zh) | 2021-10-05 | 2024-06-14 | 再生生物股份有限公司 | 用于重组aav生产的组合物和方法 |
| WO2023102517A1 (en) | 2021-12-02 | 2023-06-08 | The Trustees Of The University Of Pennsylvania | Compositions and methods for treatment of fabry disease |
| CN114317464B (zh) * | 2021-12-27 | 2023-07-04 | 武汉汇研生物科技股份有限公司 | 腺相关病毒rAAV9的分离纯化方法 |
| US20250213727A1 (en) | 2022-03-25 | 2025-07-03 | Regenxbio Inc. | Dominant-negative tumor necrosis factor alpha adeno-associated virus gene therapy |
| WO2023191827A1 (en) * | 2022-03-28 | 2023-10-05 | President And Fellows Of Harvard College | High efficiency purification of divergent aav serotypes using aavx affinity chromatography |
| WO2023239627A2 (en) | 2022-06-08 | 2023-12-14 | Regenxbio Inc. | Methods for recombinant aav production |
| US20260021205A1 (en) | 2022-07-15 | 2026-01-22 | The Trustees Of The University Of Pennsylvania | Recombinant aav having aav clade d and clade e capsids and compositions containing same |
| WO2024038365A1 (en) * | 2022-08-16 | 2024-02-22 | Pfizer Inc. | Methods for purification of aav vectors by anion exchange chromatography |
| WO2024211780A1 (en) | 2023-04-07 | 2024-10-10 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| WO2024233529A2 (en) | 2023-05-07 | 2024-11-14 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| JPWO2024242150A1 (enExample) * | 2023-05-23 | 2024-11-28 | ||
| CN121358869A (zh) * | 2023-05-25 | 2026-01-16 | 朱诺治疗学股份有限公司 | Aav纯化方法 |
| US20240425825A1 (en) * | 2023-06-20 | 2024-12-26 | Regeneron Pharmaceuticals, Inc. | Methods for purifying filled adeno-associated virus capsids |
| WO2025090962A1 (en) | 2023-10-25 | 2025-05-01 | Regenxbio Inc. | Compositions and methods for recombinant aav production |
| US20250163468A1 (en) * | 2023-11-21 | 2025-05-22 | Regeneron Pharmaceuticals, Inc. | Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus |
| EP4606892A1 (en) * | 2024-02-23 | 2025-08-27 | Merck Patent GmbH | Methods for the production of viral particles |
| WO2025226558A1 (en) * | 2024-04-22 | 2025-10-30 | Ultragenyx Pharmaceutical Inc. | Multi-step filtration and clarification methods for recombinant adeno-associated virus purification |
| WO2025228321A1 (en) * | 2024-04-29 | 2025-11-06 | Skyline Therapeutics (Shanghai) Co., Ltd. | METHOD OF SEPARATING RECOMBINANT ADENO-ASSOCIATED VIRUS (rAAV) PARTICLES |
Family Cites Families (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6989264B2 (en) | 1997-09-05 | 2006-01-24 | Targeted Genetics Corporation | Methods for generating high titer helper-free preparations of released recombinant AAV vectors |
| ATE403715T1 (de) | 1999-08-09 | 2008-08-15 | Targeted Genetics Corp | Erhöhung der expression einer einzelsträngigen, heterologen nukleotidsequenz von einem rekombinanten viralen vektor durch ausgestaltung der sequenz in einer art und weise, dass basenpaarungen innerhalb der sequenz entstehen |
| AU2911501A (en) | 1999-12-29 | 2001-07-09 | Genzyme Corporation | Method using filtration aids for the separation of virus vectors from nucleic acids and other cellular contaminants |
| WO2002000337A1 (en) | 2000-06-27 | 2002-01-03 | Bia Separations D.O.O. | A chromatography material and a process of manufacturing that material |
| US6593123B1 (en) | 2000-08-07 | 2003-07-15 | Avigen, Inc. | Large-scale recombinant adeno-associated virus (rAAV) production and purification |
| US6723551B2 (en) | 2001-11-09 | 2004-04-20 | The United States Of America As Represented By The Department Of Health And Human Services | Production of adeno-associated virus in insect cells |
| CN103555677B (zh) | 2001-11-13 | 2018-01-30 | 宾夕法尼亚大学托管会 | 检测和/或鉴定腺伴随病毒(aav)序列以及分离所鉴定的新型序列的方法 |
| WO2003052051A2 (en) | 2001-12-17 | 2003-06-26 | The Trustees Of The University Of Pennsylvania | Adeno-associated virus (aav) serotype 8 sequences |
| ES2521682T3 (es) | 2003-05-21 | 2014-11-13 | Genzyme Corporation | Procedimientos para producir preparaciones de viriones de AAV recombinantes sustancialmente exentas de cápsidas vacías |
| DK3211085T3 (da) | 2003-09-30 | 2021-06-21 | Univ Pennsylvania | Klader af adeno-associeret virus (aav), sekvenser, vektorer indeholdende disse og anvendelser deraf |
| US7183969B2 (en) | 2004-12-22 | 2007-02-27 | Raytheon Company | System and technique for calibrating radar arrays |
| US8999678B2 (en) | 2005-04-07 | 2015-04-07 | The Trustees Of The University Of Pennsylvania | Method of increasing the function of an AAV vector |
| US7456683B2 (en) | 2005-06-09 | 2008-11-25 | Panasonic Corporation | Amplitude error compensating device and quadrature skew error compensating device |
| AU2009209408B2 (en) | 2008-01-29 | 2015-06-11 | Beacon Therapeutics Limited | Recombinant virus production using mammalian cells in suspension |
| CA2715924C (en) | 2008-02-19 | 2021-01-12 | Andrew Christian BAKKER | Optimisation of expression of parvoviral rep and cap proteins in insect cells |
| ES2724122T3 (es) | 2009-04-30 | 2019-09-06 | Univ Pennsylvania | Composiciones para dirigir células de las vías respiratorias de conducción que comprenden construcciones de virus adenoasociado |
| WO2010138263A2 (en) | 2009-05-28 | 2010-12-02 | University Of Massachusetts | Novel aav 's and uses thereof |
| US8628966B2 (en) | 2010-04-30 | 2014-01-14 | City Of Hope | CD34-derived recombinant adeno-associated vectors for stem cell transduction and systemic therapeutic gene transfer |
| US8927514B2 (en) | 2010-04-30 | 2015-01-06 | City Of Hope | Recombinant adeno-associated vectors for targeted treatment |
| EP2634253B1 (en) | 2010-10-27 | 2016-05-11 | Jichi Medical University | Adeno-associated virus virions for transferring genes into neural cells |
| JP6042825B2 (ja) | 2011-02-10 | 2016-12-14 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | 改変された形質導入プロファイルを有するウイルスベクターならびにその製造および使用の方法 |
| DK4005603T3 (da) | 2011-04-22 | 2024-11-25 | Univ California | Adeno-associerede virus-virioner med variant kapsid og fremgangsmåder til anvendelse heraf |
| US9169299B2 (en) | 2011-08-24 | 2015-10-27 | The Board Of Trustees Of The Leleand Stanford Junior University | AAV capsid proteins for nucleic acid transfer |
| WO2013170078A1 (en) | 2012-05-09 | 2013-11-14 | Oregon Health & Science University | Adeno associated virus plasmids and vectors |
| EP2970946A4 (en) | 2013-03-13 | 2016-09-07 | Philadelphia Children Hospital | ADENOASSOZED VIRUS VECTORS AND METHOD FOR USE THEREOF |
| EP2986632B1 (en) | 2013-04-20 | 2018-09-05 | Research Institute at Nationwide Children's Hospital | Recombinant adeno-associated virus delivery of exon 2-targeted u7snrna polynucleotide constructs |
| CA3209883A1 (en) | 2013-07-22 | 2015-01-29 | The Children's Hospital Of Philadelphia | Variant aav and compositions, methods and uses for gene transfer to cells, organs and tissues |
| US9585971B2 (en) | 2013-09-13 | 2017-03-07 | California Institute Of Technology | Recombinant AAV capsid protein |
| ES2857751T3 (es) | 2013-10-11 | 2021-09-29 | Massachusetts Eye & Ear Infirmary | Métodos para predecir secuencias de virus ancestrales y usos de los mismos |
| WO2015164757A1 (en) | 2014-04-25 | 2015-10-29 | Oregon Health & Science University | Methods of viral neutralizing antibody epitope mapping |
| WO2015191508A1 (en) | 2014-06-09 | 2015-12-17 | Voyager Therapeutics, Inc. | Chimeric capsids |
| KR20230067694A (ko) | 2014-09-24 | 2023-05-16 | 시티 오브 호프 | 고효율 게놈 편집을 위한 아데노-관련 바이러스 벡터 변이체 및 이의 방법 |
| EP3054007A1 (en) | 2015-02-09 | 2016-08-10 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step |
| JP6665466B2 (ja) | 2015-09-26 | 2020-03-13 | 日亜化学工業株式会社 | 半導体発光素子及びその製造方法 |
| WO2017070491A1 (en) | 2015-10-23 | 2017-04-27 | Applied Genetic Technologies Corporation | Ophthalmic formulations |
| US11098286B2 (en) | 2015-12-11 | 2021-08-24 | The Trustees Of The University Of Pennsylvania | Scalable purification method for AAV9 |
| EP3387118B1 (en) | 2015-12-11 | 2022-04-06 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aavrh10 |
| WO2017100676A1 (en) * | 2015-12-11 | 2017-06-15 | The Trustees Of The University Of Pennsylvania | Scalable purification method for aav8 |
| US11015173B2 (en) * | 2015-12-11 | 2021-05-25 | The Trustees Of The University Of Pennsylvania | Scalable purification method for AAV1 |
| CN109463017A (zh) | 2016-03-14 | 2019-03-12 | 佩鲁马拉有限公司 | 间歇和连续地再循环光的光伏系统 |
| WO2019178495A1 (en) * | 2018-03-16 | 2019-09-19 | Biogen Ma Inc. | Methods for purifying recombinant adeno-associated viruses |
-
2019
- 2019-06-13 KR KR1020217000909A patent/KR102930150B1/ko active Active
- 2019-06-13 WO PCT/US2019/037013 patent/WO2019241535A2/en not_active Ceased
- 2019-06-13 AU AU2019285186A patent/AU2019285186B2/en active Active
- 2019-06-13 IL IL319002A patent/IL319002A/en unknown
- 2019-06-13 IL IL279193A patent/IL279193B2/en unknown
- 2019-06-13 EP EP19748612.9A patent/EP3807405A2/en active Pending
- 2019-06-13 CA CA3102817A patent/CA3102817A1/en active Pending
- 2019-06-13 US US17/251,869 patent/US12070702B2/en active Active
- 2019-06-13 JP JP2020568998A patent/JP7385603B2/ja active Active
- 2019-06-13 CN CN201980048303.1A patent/CN112469822A/zh active Pending
-
2024
- 2024-07-19 US US18/777,913 patent/US20250186904A1/en active Pending
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