AU2019285186B2 - Anion exchange chromatography for recombinant AAV production - Google Patents

Anion exchange chromatography for recombinant AAV production Download PDF

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Publication number
AU2019285186B2
AU2019285186B2 AU2019285186A AU2019285186A AU2019285186B2 AU 2019285186 B2 AU2019285186 B2 AU 2019285186B2 AU 2019285186 A AU2019285186 A AU 2019285186A AU 2019285186 A AU2019285186 A AU 2019285186A AU 2019285186 B2 AU2019285186 B2 AU 2019285186B2
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Prior art keywords
aav
raav particles
feed composition
raav
particles
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AU2019285186A1 (en
Inventor
Franz M. GERNER
Jeffrey Levine
Sara ZAFAR
Claire G. ZHANG
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Regenxbio Inc
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Regenxbio Inc
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2019285186A 2018-06-14 2019-06-13 Anion exchange chromatography for recombinant AAV production Active AU2019285186B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862684835P 2018-06-14 2018-06-14
US62/684,835 2018-06-14
PCT/US2019/037013 WO2019241535A2 (en) 2018-06-14 2019-06-13 Anion exchange chromatography for recombinant aav production

Publications (2)

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AU2019285186A1 AU2019285186A1 (en) 2021-01-07
AU2019285186B2 true AU2019285186B2 (en) 2025-05-29

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AU2019285186A Active AU2019285186B2 (en) 2018-06-14 2019-06-13 Anion exchange chromatography for recombinant AAV production

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US (2) US12070702B2 (enExample)
EP (1) EP3807405A2 (enExample)
JP (1) JP7385603B2 (enExample)
KR (1) KR102930150B1 (enExample)
CN (1) CN112469822A (enExample)
AU (1) AU2019285186B2 (enExample)
CA (1) CA3102817A1 (enExample)
IL (2) IL319002A (enExample)
WO (1) WO2019241535A2 (enExample)

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US20240408157A1 (en) 2021-12-02 2024-12-12 The Trustees Of The University Of Pennsylvania Compositions and methods for treatment of fabry disease
CN114317464B (zh) * 2021-12-27 2023-07-04 武汉汇研生物科技股份有限公司 腺相关病毒rAAV9的分离纯化方法
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WO2023239627A2 (en) 2022-06-08 2023-12-14 Regenxbio Inc. Methods for recombinant aav production
US20260021205A1 (en) 2022-07-15 2026-01-22 The Trustees Of The University Of Pennsylvania Recombinant aav having aav clade d and clade e capsids and compositions containing same
WO2024038365A1 (en) * 2022-08-16 2024-02-22 Pfizer Inc. Methods for purification of aav vectors by anion exchange chromatography
WO2024211780A1 (en) 2023-04-07 2024-10-10 Regenxbio Inc. Compositions and methods for recombinant aav production
WO2024233529A2 (en) 2023-05-07 2024-11-14 Regenxbio Inc. Compositions and methods for recombinant aav production
WO2024242150A1 (ja) * 2023-05-23 2024-11-28 東ソー株式会社 改変された組換えアデノ随伴ウイルス（ａａｖ）結合性タンパク質およびａａｖの精製方法
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Also Published As

Publication number Publication date
IL279193B1 (en) 2025-03-01
JP2021526829A (ja) 2021-10-11
US20250186904A1 (en) 2025-06-12
WO2019241535A2 (en) 2019-12-19
CN112469822A (zh) 2021-03-09
IL279193B2 (en) 2025-07-01
US12070702B2 (en) 2024-08-27
CA3102817A1 (en) 2019-12-19
AU2019285186A1 (en) 2021-01-07
JP7385603B2 (ja) 2023-11-22
US20210370199A1 (en) 2021-12-02
KR102930150B1 (ko) 2026-02-25
IL279193A (en) 2021-01-31
IL319002A (en) 2025-04-01
EP3807405A2 (en) 2021-04-21
KR20210020100A (ko) 2021-02-23
WO2019241535A3 (en) 2020-01-23

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