JPWO2017144900A5 - - Google Patents
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- Publication number
- JPWO2017144900A5 JPWO2017144900A5 JP2018544901A JP2018544901A JPWO2017144900A5 JP WO2017144900 A5 JPWO2017144900 A5 JP WO2017144900A5 JP 2018544901 A JP2018544901 A JP 2018544901A JP 2018544901 A JP2018544901 A JP 2018544901A JP WO2017144900 A5 JPWO2017144900 A5 JP WO2017144900A5
- Authority
- JP
- Japan
- Prior art keywords
- fragment
- immunoglobulin
- light chain
- antibody
- heavy chain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000012634 fragment Substances 0.000 claims description 66
- 108060003951 Immunoglobulin Proteins 0.000 claims description 30
- 102000018358 immunoglobulin Human genes 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 21
- 230000027455 binding Effects 0.000 claims description 5
- 229940072221 immunoglobulins Drugs 0.000 claims description 5
- 238000004132 cross linking Methods 0.000 claims description 4
- AQSJGOWTSHOLKH-UHFFFAOYSA-N phosphite(3-) Chemical class [O-]P([O-])[O-] AQSJGOWTSHOLKH-UHFFFAOYSA-N 0.000 claims description 4
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 claims description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 claims description 2
- 239000012491 analyte Substances 0.000 claims 12
- 238000004949 mass spectrometry Methods 0.000 claims 10
- 239000000523 sample Substances 0.000 claims 10
- 238000003149 assay kit Methods 0.000 claims 6
- 201000010099 disease Diseases 0.000 claims 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 3
- SOYPUUFPUFRXRI-UHFFFAOYSA-N 1,5-dibromo-2,4-dimethylbenzene Chemical group CC1=CC(C)=C(Br)C=C1Br SOYPUUFPUFRXRI-UHFFFAOYSA-N 0.000 claims 2
- 206010034962 Photopsia Diseases 0.000 claims 2
- 150000001413 amino acids Chemical group 0.000 claims 2
- 150000001541 aziridines Chemical class 0.000 claims 2
- 210000003719 b-lymphocyte Anatomy 0.000 claims 2
- 150000004820 halides Chemical class 0.000 claims 2
- 125000005179 haloacetyl group Chemical group 0.000 claims 2
- 229910000065 phosphene Inorganic materials 0.000 claims 2
- 229920003192 poly(bis maleimide) Polymers 0.000 claims 2
- 239000012521 purified sample Substances 0.000 claims 2
- 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 claims 1
- 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 238000004393 prognosis Methods 0.000 claims 1
- 238000011002 quantification Methods 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 238000003556 assay Methods 0.000 description 6
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- XYFCBTPGUUZFHI-UHFFFAOYSA-N Phosphine Chemical compound P XYFCBTPGUUZFHI-UHFFFAOYSA-N 0.000 description 2
- 238000000149 argon plasma sintering Methods 0.000 description 2
- FDIOSTIIZGWENY-UHFFFAOYSA-N n-[bis(diethylamino)phosphanyl]-n-ethylethanamine Chemical compound CCN(CC)P(N(CC)CC)N(CC)CC FDIOSTIIZGWENY-UHFFFAOYSA-N 0.000 description 2
- XVDBWWRIXBMVJV-UHFFFAOYSA-N n-[bis(dimethylamino)phosphanyl]-n-methylmethanamine Chemical compound CN(C)P(N(C)C)N(C)C XVDBWWRIXBMVJV-UHFFFAOYSA-N 0.000 description 2
- 238000004848 nephelometry Methods 0.000 description 2
- TUQOTMZNTHZOKS-UHFFFAOYSA-N tributylphosphine Chemical compound CCCCP(CCCC)CCCC TUQOTMZNTHZOKS-UHFFFAOYSA-N 0.000 description 2
- CYTQBVOFDCPGCX-UHFFFAOYSA-N trimethyl phosphite Chemical compound COP(OC)OC CYTQBVOFDCPGCX-UHFFFAOYSA-N 0.000 description 2
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- 238000011088 calibration curve Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000012038 nucleophile Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 125000004430 oxygen atom Chemical group O* 0.000 description 1
- 150000003003 phosphines Chemical class 0.000 description 1
- 229910000073 phosphorus hydride Inorganic materials 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- ISIJQEHRDSCQIU-UHFFFAOYSA-N tert-butyl 2,7-diazaspiro[4.5]decane-7-carboxylate Chemical compound C1N(C(=O)OC(C)(C)C)CCCC11CNCC1 ISIJQEHRDSCQIU-UHFFFAOYSA-N 0.000 description 1
- 150000003568 thioethers Chemical class 0.000 description 1
- 238000004879 turbidimetry Methods 0.000 description 1
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022017497A JP7541045B2 (ja) | 2016-02-25 | 2022-02-07 | 質量分析キット |
| JP2022198753A JP7541070B2 (ja) | 2016-02-25 | 2022-12-13 | 質量分析キット |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1603291.4A GB201603291D0 (en) | 2016-02-25 | 2016-02-25 | Antibodies |
| GB1603291.4 | 2016-02-25 | ||
| US201662368606P | 2016-07-29 | 2016-07-29 | |
| US62/368,606 | 2016-07-29 | ||
| PCT/GB2017/050489 WO2017144900A1 (en) | 2016-02-25 | 2017-02-24 | Mass spectrometry kit |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022017497A Division JP7541045B2 (ja) | 2016-02-25 | 2022-02-07 | 質量分析キット |
| JP2022198753A Division JP7541070B2 (ja) | 2016-02-25 | 2022-12-13 | 質量分析キット |
Publications (4)
| Publication Number | Publication Date |
|---|---|
| JP2019509483A JP2019509483A (ja) | 2019-04-04 |
| JP2019509483A5 JP2019509483A5 (https=) | 2022-12-26 |
| JPWO2017144900A5 true JPWO2017144900A5 (https=) | 2022-12-26 |
| JP7246927B2 JP7246927B2 (ja) | 2023-03-28 |
Family
ID=55806928
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018544901A Active JP7246927B2 (ja) | 2016-02-25 | 2017-02-24 | 質量分析キット |
| JP2022017497A Active JP7541045B2 (ja) | 2016-02-25 | 2022-02-07 | 質量分析キット |
| JP2022198753A Active JP7541070B2 (ja) | 2016-02-25 | 2022-12-13 | 質量分析キット |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022017497A Active JP7541045B2 (ja) | 2016-02-25 | 2022-02-07 | 質量分析キット |
| JP2022198753A Active JP7541070B2 (ja) | 2016-02-25 | 2022-12-13 | 質量分析キット |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US12461108B2 (https=) |
| EP (2) | EP4123308A1 (https=) |
| JP (3) | JP7246927B2 (https=) |
| KR (1) | KR102851057B1 (https=) |
| CN (2) | CN116148479A (https=) |
| AU (1) | AU2017222399B2 (https=) |
| DK (1) | DK3420361T3 (https=) |
| ES (1) | ES2936783T3 (https=) |
| GB (1) | GB201603291D0 (https=) |
| WO (1) | WO2017144900A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201708262D0 (en) | 2017-05-23 | 2017-07-05 | Binding Site Group Ltd | Assay for plasma cell associated disease |
| CA3126712A1 (en) * | 2019-01-31 | 2020-08-06 | Regeneron Pharmaceuticals, Inc. | Native microfluidic ce-ms analysis of antibody charge heterogeneity |
| CN110009008B (zh) * | 2019-03-18 | 2020-11-06 | 四川大学 | 基于提取的免疫固定电泳图特征对其进行自动分类的方法 |
| GB201906599D0 (en) | 2019-05-10 | 2019-06-26 | Binding Site Group Ltd | Mass spectrometry calibrator |
| WO2020229794A1 (en) | 2019-05-10 | 2020-11-19 | The Binding Site Group Limited | Mass spectrometry calibrator |
| GB201915946D0 (en) | 2019-11-01 | 2019-12-18 | Binding Site Group Ltd | Quantitation of immunoglobulins i n mass spectrometry |
| GB202007047D0 (en) * | 2020-05-13 | 2020-06-24 | Binding Site Group Ltd | Mass spectrometry controls |
| GB202008887D0 (en) * | 2020-06-11 | 2020-07-29 | Binding Site Group Ltd | A method of identifying or characterising an immune response in a subject |
| GB202016822D0 (en) | 2020-10-23 | 2020-12-09 | Binding Site Group Ltd | Quantitation of immunoglobulins in mass spectrometry |
| CN113720900A (zh) * | 2021-09-14 | 2021-11-30 | 首都医科大学附属北京朝阳医院 | 一种基于madli-tof ms技术检测血清中m蛋白的方法 |
| CN114324557B (zh) * | 2021-12-03 | 2024-05-10 | 融智生物科技（青岛）有限公司 | 一种基于MALDI-TOF MS的ζ-珠蛋白的检测方法 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU6290090A (en) | 1989-08-29 | 1991-04-08 | University Of Southampton | Bi-or trispecific (fab)3 or (fab)4 conjugates |
| WO1991006570A1 (en) * | 1989-10-25 | 1991-05-16 | The University Of Melbourne | HYBRID Fc RECEPTOR MOLECULES |
| EP1400536A1 (en) * | 1991-06-14 | 2004-03-24 | Genentech Inc. | Method for making humanized antibodies |
| ES2242189T3 (es) * | 1994-05-13 | 2005-11-01 | Miltenyi Biotec Gmbh | Columna esteril y apirogena copulada a una proteina con miras a la fijacion y extraccion de sustancias dadas de la sangre. |
| JP2826965B2 (ja) * | 1995-01-12 | 1998-11-18 | オリエンタル酵母工業株式会社 | 免疫吸着体とその製造法 |
| EP0862584B1 (en) | 1995-11-03 | 2004-12-22 | The Binding Site Limited | Production of antibodies |
| US6207370B1 (en) * | 1997-09-02 | 2001-03-27 | Sequenom, Inc. | Diagnostics based on mass spectrometric detection of translated target polypeptides |
| US6897044B1 (en) | 1999-01-28 | 2005-05-24 | Biogen Idec, Inc. | Production of tetravalent antibodies |
| WO2005035721A2 (en) * | 2003-10-08 | 2005-04-21 | Ebioscience | Native immunoglobulin binding reagents and methods for making and using same |
| GB0501741D0 (en) * | 2005-01-27 | 2005-03-02 | Binding Site The Ltd | Antibody |
| CA2600844A1 (en) * | 2005-03-14 | 2006-09-21 | Medimmune, Inc. | Macromolecules comprising a thioether cross-link |
| US7781178B2 (en) | 2005-04-12 | 2010-08-24 | Akira Matsumori | Biomarker for diagnosing heart disease and the use thereof |
| US7893616B2 (en) * | 2006-02-27 | 2011-02-22 | Ohio State University | Process to determine enzyme activity |
| US20100015652A1 (en) | 2006-12-21 | 2010-01-21 | Brian Walter Granda | Antibody quantitation |
| RU2012118620A (ru) | 2009-10-09 | 2013-11-20 | Симфоген А/С | Множественное количественное определение в смеси индивидуальных рекомбинантных белков посредством сигнатурных пептидов и масс-спектрометрии |
| GB201001946D0 (en) | 2010-02-05 | 2010-03-24 | Binding Site Group The Ltd | Cancer prognostic assay |
| GB2478520A (en) | 2010-03-02 | 2011-09-14 | Binding Site Group Ltd | Kidney prognostic assay |
| GB201117172D0 (en) | 2011-10-05 | 2011-11-16 | Binding Site Group The Ltd | Diabetes |
| GB201121265D0 (en) | 2011-12-12 | 2012-01-18 | Binding Site Group The Ltd | Assay |
| JP2015510877A (ja) * | 2012-03-09 | 2015-04-13 | ユーシーエル ビジネス パブリック リミテッド カンパニー | 抗体の化学修飾 |
| WO2014121031A1 (en) | 2013-01-31 | 2014-08-07 | Excelimmune, Inc. | Characterization of antibody mixtures by mass spectrometry |
| WO2015131169A2 (en) * | 2014-02-28 | 2015-09-03 | H. Lee Moffitt Cancer Center And Research Institute, Inc. | Personalized myeloma detection |
| WO2015154052A1 (en) | 2014-04-04 | 2015-10-08 | Mayo Foundation For Medical Education And Research | Isotyping immunoglobulins using accurate molecular mass |
| EP3175242A4 (en) * | 2014-07-29 | 2017-12-27 | Mayo Foundation for Medical Education and Research | Quantifying monoclonal antibody therapeutics by lc-ms/ms |
| PT3209767T (pt) * | 2014-10-21 | 2020-11-09 | Gennova Biopharmaceuticals Ltd | Um novo processo de purificação para isolamento e produção comercial de tnk-tpa recombinante (tenecteplase) |
| CN108026164A (zh) | 2015-09-24 | 2018-05-11 | 梅约医学教育与研究基金会 | 通过质谱鉴定免疫球蛋白游离轻链 |
| GB201708262D0 (en) * | 2017-05-23 | 2017-07-05 | Binding Site Group Ltd | Assay for plasma cell associated disease |
-
2016
- 2016-02-25 GB GBGB1603291.4A patent/GB201603291D0/en not_active Ceased
-
2017
- 2017-02-24 CN CN202211574983.0A patent/CN116148479A/zh active Pending
- 2017-02-24 WO PCT/GB2017/050489 patent/WO2017144900A1/en not_active Ceased
- 2017-02-24 AU AU2017222399A patent/AU2017222399B2/en active Active
- 2017-02-24 US US16/078,986 patent/US12461108B2/en active Active
- 2017-02-24 JP JP2018544901A patent/JP7246927B2/ja active Active
- 2017-02-24 ES ES17708320T patent/ES2936783T3/es active Active
- 2017-02-24 EP EP22190012.9A patent/EP4123308A1/en active Pending
- 2017-02-24 KR KR1020187027703A patent/KR102851057B1/ko active Active
- 2017-02-24 CN CN201780013491.5A patent/CN108885218A/zh active Pending
- 2017-02-24 EP EP17708320.1A patent/EP3420361B1/en active Active
- 2017-02-24 DK DK17708320.1T patent/DK3420361T3/da active
-
2022
- 2022-02-07 JP JP2022017497A patent/JP7541045B2/ja active Active
- 2022-12-13 JP JP2022198753A patent/JP7541070B2/ja active Active
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