JPWO2015182382A1 - 細胞評価装置および方法並びにプログラム - Google Patents
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Abstract
Description
2 撮像装置
3 細胞評価装置
4 ディスプレイ
5 入力装置
10 ステージ
11 搬送部
12 制御部
15 培養容器
20 位相差顕微鏡
21 照明光源
22 スリット板
23 対物レンズ
24 位相差レンズ
25 対物レンズ
26 位相板
27 結像レンズ
28 撮像素子
29 制御部
30 画像取得部
31 境界設定部
32 細胞評価部
33 表示制御部
34 撮像条件取得部
35 撮像制御部
Claims (13)
- 細胞群を撮像した細胞画像を取得する画像取得部と、
前記細胞群における個々の細胞を認識し、該認識した個々の細胞のうちの評価対象の細胞と該評価対象の細胞の周囲の細胞とを特定し、前記周囲の細胞の評価結果と、前記評価対象の細胞の評価結果とに基づいて、前記評価対象の細胞の評価を確定する細胞評価部とを備えることを特徴とする細胞評価装置。 - 前記細胞評価部が、前記周囲の細胞の評価結果のうち、評価が可能な細胞の評価結果のみに基づいて、前記評価対象の細胞の評価を確定する請求項1記載の細胞評価装置。
- 前記細胞評価部が、前記評価対象の細胞の細胞核の大きさまたは形状、細胞質の大きさまたは形状、核小体の大きさまたは形状、前記細胞の増殖速度および遊走速度の少なくとも1つに基づいて、前記周囲の細胞を特定するための領域を決定し、該領域に全部または一部が含まれる細胞を周囲の細胞として特定する請求項1または2記載の細胞評価装置。
- 前記細胞評価部が、前記周囲の細胞を特定するための領域に存在する細胞の数が予め設定した数に到達するまで前記領域を広げる請求項3記載の細胞評価装置。
- 前記細胞評価部が、前記細胞画像の縦方向および横方向についてそれぞれ前記領域を広げる請求項4記載の細胞評価装置。
- 前記細胞評価部が、前記領域の前記縦方向および前記横方向のアスペクト比を決定する請求項5記載の細胞評価装置。
- 前記細胞評価部が、前記領域を広げる際、前記縦方向に探索する細胞数及び前記横方向に探索する細胞数を決定する請求項5記載の細胞評価装置。
- 前記細胞評価部が、前記評価対象の細胞と前記周囲の細胞との距離に基づいて、前記周囲の細胞の評価結果に対して重み付けを設定する請求項1から7いずれか1項記載の細胞評価装置。
- 前記細胞評価部が、前記評価対象の細胞の未分化状態または分化状態を評価する請求項1から8いずれか1項記載の細胞評価装置。
- 前記細胞評価部が、前記評価対象の細胞の分化度を評価する請求項1から8いずれか1項記載の細胞評価装置。
- 前記細胞群の状態に基づいて、前記細胞画像に境界を設定する境界設定部を備え、
前記細胞評価部が、前記周囲の細胞を特定する際、前記境界によって分割される複数の分割領域のうち前記評価対象の細胞が存在する分割領域内に存在する細胞のみを前記周囲の細胞として特定する請求項1から10いずれか1項記載の細胞評価装置。 - 細胞群を撮像した細胞画像を取得し、
前記細胞群における個々の細胞を認識し、該認識した個々の細胞のうちの評価対象の細胞と該評価対象の細胞の周囲の細胞とを特定し、前記周囲の細胞の評価結果と、前記評価対象の細胞の評価結果とに基づいて、前記評価対象の細胞の評価を確定することを特徴とする細胞評価方法。 - コンピュータを、細胞群を撮像した細胞画像を取得する画像取得部と、
前記細胞群における個々の細胞を認識し、該認識した個々の細胞のうちの評価対象の細胞と該評価対象の細胞の周囲の細胞とを特定し、前記周囲の細胞の評価結果と、前記評価対象の細胞の評価結果とに基づいて、前記評価対象の細胞の評価を確定する細胞評価部として機能させることを特徴とする細胞評価プログラム。
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JP6143365B2 (ja) * | 2014-03-05 | 2017-06-07 | 富士フイルム株式会社 | 細胞画像評価装置および方法並びにプログラム |
JP6301199B2 (ja) * | 2014-05-30 | 2018-03-28 | 富士フイルム株式会社 | 細胞評価装置および方法並びにプログラム |
WO2015182382A1 (ja) * | 2014-05-30 | 2015-12-03 | 富士フイルム株式会社 | 細胞評価装置および方法並びにプログラム |
JP6475134B2 (ja) | 2015-09-29 | 2019-02-27 | 富士フイルム株式会社 | 細胞評価装置および方法 |
JP6777147B2 (ja) * | 2016-06-30 | 2020-10-28 | 株式会社ニコン | 画像選択装置、画像選択プログラム、演算装置、及び表示装置 |
KR102299378B1 (ko) * | 2016-08-24 | 2021-09-07 | 구글 엘엘씨 | 변화 검출 기반의 맵 인터페이스 업데이트 시스템 |
JP6801000B2 (ja) * | 2016-12-01 | 2020-12-16 | 富士フイルム株式会社 | 細胞画像評価装置および細胞画像評価制御プログラム |
EP3553165A4 (en) | 2016-12-06 | 2019-12-25 | Fujifilm Corporation | CELL IMAGE EVALUATION DEVICE AND CELL IMAGE EVALUATION CONTROL PROGRAM |
KR102261700B1 (ko) | 2017-03-30 | 2021-06-04 | 후지필름 가부시키가이샤 | 세포 화상 평가 장치 및 방법과, 프로그램 |
US11321585B2 (en) | 2017-04-14 | 2022-05-03 | Hitachi High-Tech Corporation | Imaging device and morphological feature data display method |
EP3675835A4 (en) | 2017-08-28 | 2021-06-09 | Matthias Wagner | MICROFLUIDIC LASER ACTIVATED INTRACELLULAR DELIVERY SYSTEMS AND METHODS |
WO2019135266A1 (ja) * | 2018-01-04 | 2019-07-11 | オリンパス株式会社 | 細胞画像処理装置、細胞画像処理方法および細胞画像処理プログラム |
JPWO2019176048A1 (ja) * | 2018-03-15 | 2021-02-25 | オリンパス株式会社 | 細胞画像処理装置 |
GB2615908B (en) * | 2018-06-28 | 2023-11-08 | Nikon Corp | Device, microscope device, method, and program |
JP7248024B2 (ja) | 2018-06-28 | 2023-03-29 | 株式会社ニコン | 細胞状態評価装置、顕微鏡装置、細胞状態評価方法およびプログラム |
EP3833959A4 (en) * | 2018-08-10 | 2022-06-29 | Cellino Biotech, Inc. | System for image-driven cell manufacturing |
JP7274936B2 (ja) * | 2019-05-24 | 2023-05-17 | 富士フイルム株式会社 | 細胞画像圧縮装置、方法およびプログラム |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2001269195A (ja) * | 2000-03-27 | 2001-10-02 | Olympus Optical Co Ltd | 細胞の画像解析方法,装置、及び記録媒体 |
JP2011229409A (ja) * | 2010-04-23 | 2011-11-17 | Nagoya Univ | 細胞評価装置、インキュベータ、細胞評価方法、細胞評価プログラムおよび細胞の培養方法 |
JP2014039504A (ja) * | 2012-08-23 | 2014-03-06 | Fuji Xerox Co Ltd | 画像処理装置、プログラム及び画像処理システム |
Family Cites Families (11)
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US3597297A (en) | 1968-06-25 | 1971-08-03 | Minnesota Mining & Mfg | Synthetic turf material and method of making same |
US7117098B1 (en) * | 1997-02-27 | 2006-10-03 | Cellomics, Inc. | Machine-readable storage medium for analyzing distribution of macromolecules between the cell membrane and the cell cytoplasm |
AU2073801A (en) * | 1999-12-09 | 2001-06-18 | Cellomics, Inc. | A system for cell-based screening |
JPWO2006057444A1 (ja) * | 2004-11-25 | 2008-06-05 | 国立大学法人 北海道大学 | 細胞の分化度自動診断方法 |
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US9014444B2 (en) * | 2012-07-13 | 2015-04-21 | Sony Corporation | Method and apparatus for automatic HER2 scoring of tissue samples |
JP2014018184A (ja) * | 2012-07-23 | 2014-02-03 | Tokyo Electron Ltd | 画像解析による多能性幹細胞の評価方法 |
WO2015182382A1 (ja) * | 2014-05-30 | 2015-12-03 | 富士フイルム株式会社 | 細胞評価装置および方法並びにプログラム |
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
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JP2011229409A (ja) * | 2010-04-23 | 2011-11-17 | Nagoya Univ | 細胞評価装置、インキュベータ、細胞評価方法、細胞評価プログラムおよび細胞の培養方法 |
JP2014039504A (ja) * | 2012-08-23 | 2014-03-06 | Fuji Xerox Co Ltd | 画像処理装置、プログラム及び画像処理システム |
Non-Patent Citations (1)
Title |
---|
INTERNATIONAL CONFERENCE ON ADVANCED ELECTRONIC SYSTEMS(ICAES), JPN6018012304, 2013, pages 243 - 247, ISSN: 0003779858 * |
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