JPS6486871A - Method for separating and purifying recombinant protein - Google Patents

Method for separating and purifying recombinant protein

Info

Publication number
JPS6486871A
JPS6486871A JP24701787A JP24701787A JPS6486871A JP S6486871 A JPS6486871 A JP S6486871A JP 24701787 A JP24701787 A JP 24701787A JP 24701787 A JP24701787 A JP 24701787A JP S6486871 A JPS6486871 A JP S6486871A
Authority
JP
Japan
Prior art keywords
microbial cells
chromatography
escherichia coli
bacillus subtilis
recombinant plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP24701787A
Other languages
English (en)
Japanese (ja)
Other versions
JPH0333315B2 (enExample
Inventor
Masahiro Iwakura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology
Original Assignee
Agency of Industrial Science and Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency of Industrial Science and Technology filed Critical Agency of Industrial Science and Technology
Priority to JP24701787A priority Critical patent/JPS6486871A/ja
Publication of JPS6486871A publication Critical patent/JPS6486871A/ja
Publication of JPH0333315B2 publication Critical patent/JPH0333315B2/ja
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP24701787A 1987-09-30 1987-09-30 Method for separating and purifying recombinant protein Granted JPS6486871A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP24701787A JPS6486871A (en) 1987-09-30 1987-09-30 Method for separating and purifying recombinant protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP24701787A JPS6486871A (en) 1987-09-30 1987-09-30 Method for separating and purifying recombinant protein

Publications (2)

Publication Number Publication Date
JPS6486871A true JPS6486871A (en) 1989-03-31
JPH0333315B2 JPH0333315B2 (enExample) 1991-05-16

Family

ID=17157159

Family Applications (1)

Application Number Title Priority Date Filing Date
JP24701787A Granted JPS6486871A (en) 1987-09-30 1987-09-30 Method for separating and purifying recombinant protein

Country Status (1)

Country Link
JP (1) JPS6486871A (enExample)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100401517C (zh) 2000-07-19 2008-07-09 新藤电子工业株式会社 半导体装置

Also Published As

Publication number Publication date
JPH0333315B2 (enExample) 1991-05-16

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Legal Events

Date Code Title Description
EXPY Cancellation because of completion of term