JPS646856A - Enzyme immobilized electrode and its production - Google Patents
Enzyme immobilized electrode and its productionInfo
- Publication number
- JPS646856A JPS646856A JP62163043A JP16304387A JPS646856A JP S646856 A JPS646856 A JP S646856A JP 62163043 A JP62163043 A JP 62163043A JP 16304387 A JP16304387 A JP 16304387A JP S646856 A JPS646856 A JP S646856A
- Authority
- JP
- Japan
- Prior art keywords
- electrode
- enzyme
- org
- metallic film
- thin metallic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
PURPOSE:To execute the transfer and reception of electrons between enzyme and electrode with high efficiency without requiring a mediator by dispersing and holding fine metal particles into an org. matrix formed on a thin metallic film electrode. CONSTITUTION:The thin metallic film electrode 2 is formed on the surface of a semiconductor substrate 1 and the parts except said electrode are coated with a non-conductive material 3. The semiconductor substrate 1, a counter electrode 9 and a reference electrode 10 are immersed into a soln. 8 contg. the enzyme and org. monomer metal ions. A prescribed voltage is impressed between the thin metallic film electrode 2 and the counter electrode 9 with the reference electrode 10 as a reference by a potentiostat 11 to electrolytically polymerize the org. monomer in the soln. 8. Light 12 is simultaneously projected from the outside on the semiconductor substrate 1, by which the metal ions in the soln. 8 are reduced. The org. matrix 4 is formed on the surface of the thin metallic film electrode 2 and the fine metal particles 5 and the enzyme 6 are taken therein. The enzyme immobilized electrode in which said particles and enzyme are nearly uniformly dispersed and held is thus obtd.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62163043A JPS646856A (en) | 1987-06-30 | 1987-06-30 | Enzyme immobilized electrode and its production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP62163043A JPS646856A (en) | 1987-06-30 | 1987-06-30 | Enzyme immobilized electrode and its production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS646856A true JPS646856A (en) | 1989-01-11 |
Family
ID=15766098
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP62163043A Pending JPS646856A (en) | 1987-06-30 | 1987-06-30 | Enzyme immobilized electrode and its production |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS646856A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007127620A (en) * | 2005-11-01 | 2007-05-24 | Development Center For Biotechnology | Method for detecting agricultural chemical and bio micro sensor |
| JP2020507773A (en) * | 2017-02-17 | 2020-03-12 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | Sustained analyte monitoring electrodes using cross-linked enzymes |
-
1987
- 1987-06-30 JP JP62163043A patent/JPS646856A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007127620A (en) * | 2005-11-01 | 2007-05-24 | Development Center For Biotechnology | Method for detecting agricultural chemical and bio micro sensor |
| JP2020507773A (en) * | 2017-02-17 | 2020-03-12 | エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト | Sustained analyte monitoring electrodes using cross-linked enzymes |
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