JPS63500672A - 結合し得る物質の比色検定 - Google Patents

結合し得る物質の比色検定

Info

Publication number
JPS63500672A
JPS63500672A JP60500467A JP50046785A JPS63500672A JP S63500672 A JPS63500672 A JP S63500672A JP 60500467 A JP60500467 A JP 60500467A JP 50046785 A JP50046785 A JP 50046785A JP S63500672 A JPS63500672 A JP S63500672A
Authority
JP
Japan
Prior art keywords
solution
enzyme
antibody
antigen
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP60500467A
Other languages
English (en)
Japanese (ja)
Inventor
ガーバー，ベゴ
ブロック，エリオット
バハール，イザク
カンタロウ，ウオルター　デー
イートン，シリエル
ジヨンズ，ウエンデイ　イー
コセオ，メリー
ブルインズ，ジョン　ビー
Original Assignee
ハイジエイア　サイエンシ−ズ，インコ−ポレ−テツド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ハイジエイア　サイエンシ−ズ，インコ−ポレ−テツド filed Critical ハイジエイア　サイエンシ−ズ，インコ−ポレ−テツド
Publication of JPS63500672A publication Critical patent/JPS63500672A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
    • C12Q2326/10—Benzidines
    • C12Q2326/12—3,3',5,5'-Tetramethylbenzidine, i.e. TMB
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/964—Chemistry: molecular biology and microbiology including enzyme-ligand conjugate production, e.g. reducing rate of nonproductive linkage
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/815—Test for named compound or class of compounds
    • Y10S436/817—Steroids or hormones
    • Y10S436/818—Human chorionic gonadotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Reproductive Health (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP60500467A 1982-08-20 1985-01-17 結合し得る物質の比色検定 Pending JPS63500672A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/410,157 US4503143A (en) 1982-08-20 1982-08-20 Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen
PCT/US1985/000073 WO1986004421A1 (en) 1982-08-20 1985-01-17 Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen

Publications (1)

Publication Number Publication Date
JPS63500672A true JPS63500672A (ja) 1988-03-10

Family

ID=35788194

Family Applications (1)

Application Number Title Priority Date Filing Date
JP60500467A Pending JPS63500672A (ja) 1982-08-20 1985-01-17 結合し得る物質の比色検定

Country Status (5)

Country Link
US (1) US4503143A ( )
EP (1) EP0207936A4 ( )
JP (1) JPS63500672A ( )
DE (1) DE3590722T1 ( )
WO (1) WO1986004421A1 ( )

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5622871A (en) * 1987-04-27 1997-04-22 Unilever Patent Holdings B.V. Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen
JPS6152300A (ja) * 1984-08-22 1986-03-14 Eiken Kagaku Kk 過酸化水素測定用組成物
GB8505899D0 (en) * 1985-03-07 1985-04-11 Boots Celltech Diagnostics Assay reagents
US4824784A (en) * 1985-04-08 1989-04-25 Hygeia Sciences, Incorporated Chromogenic solution for immunoassay
US4931385A (en) * 1985-06-24 1990-06-05 Hygeia Sciences, Incorporated Enzyme immunoassays and immunologic reagents
US4863875A (en) * 1985-08-26 1989-09-05 Gia Research Company, L.P. Dye labelled antibodies as reagents for use in immunoassay systems
US4794076A (en) * 1985-09-23 1988-12-27 Vxr, Inc. Simultaneous extraction of a ligand from a sample and capture by anti-ligands therefor in ligand/anti-ligand assays
US4789630A (en) * 1985-10-04 1988-12-06 Cetus Corporation Ionic compounds containing the cationic meriquinone of a benzidine
DE3541979A1 (de) * 1985-11-28 1987-06-04 Behringwerke Ag Mittel zur bestimmung von peroxidase-aktivitaet mit stabilisator, verfahren zur herstellung und seine verwendung
DE3541978A1 (de) * 1985-11-28 1987-06-04 Behringwerke Ag Mittel zur bestimmung von peroxidase-aktivitaet, verfahren zur herstellung und seine verwendung
US4868108A (en) * 1985-12-12 1989-09-19 Hygeia Sciences, Incorporated Multiple-antibody detection of antigen
US4803155A (en) * 1986-02-27 1989-02-07 Agri-Diagnostics Associates Monoclonal antibodies to Sclerotinia homoeocarpa
US5187064A (en) * 1986-02-27 1993-02-16 Agri-Diagnostics Associates Monoclonal antibodies and methods for fungal pathogen detection
US4948723A (en) * 1987-01-16 1990-08-14 Bioscience International, Inc. Method of diagnosis and severity-assessment of pancreatic disease
JPS6348457A (ja) * 1986-08-19 1988-03-01 Fuji Photo Film Co Ltd 乾式多層分析要素
US4939096A (en) * 1986-09-10 1990-07-03 Idexx, Corp. Method and apparatus for assaying whole blood
DE3641830A1 (de) * 1986-12-08 1988-06-09 Fraunhofer Ges Forschung Immunenzymatischer test mit detektion durch farbumschlag im sichtbaren spektralbereich
US4954435A (en) * 1987-01-12 1990-09-04 Becton, Dickinson And Company Indirect colorimetric detection of an analyte in a sample using ratio of light signals
FR2611913B1 (fr) * 1987-02-24 1994-04-15 Khayat David Procede de dosage des recepteurs fc gamma solubles seriques, coffret de dosage correspondant et applications
EP0560411B1 (en) 1987-04-27 2000-07-26 Unilever N.V. Specific binding assays
JPH02501351A (ja) * 1987-06-09 1990-05-17 アントックス・インコーポレーテッド 芳香環含有化合物のための免疫検定
US4908305A (en) * 1987-06-14 1990-03-13 The University Of Maryland Competitive elisa for determination of neutralizing IBDV antibody
US5024935A (en) * 1987-12-18 1991-06-18 Eastman Kodak Company Dye-providing composition, diagnostic test kit and their use in method for ligand determination using peroxidase labeled-receptor
FR2621128B1 (fr) * 1987-09-30 1994-05-06 Sanofi Trousse et methode de dosage immunometrique applicables a des cellules entieres
US6461825B1 (en) 1987-09-30 2002-10-08 Sanofi (Societe Anonyme) Immunometric assay kit and method applicable to whole cells
US5093265A (en) * 1987-12-16 1992-03-03 Genesis Systems Corporation Method for detecting of premenstrual syndrome
US4870007A (en) * 1987-12-18 1989-09-26 Eastman Kodak Company Immobilized biotinylated receptor in test device, kit and method for determining a ligand
US4959303A (en) * 1987-12-21 1990-09-25 Syntex (U.S.A.) Inc. Assay for antigens by binding immune complexes to solid supports free of protein and non-ionic binders
AU2684488A (en) 1988-06-27 1990-01-04 Carter-Wallace, Inc. Test device and method for colored particle immunoassay
US5102788A (en) * 1988-11-21 1992-04-07 Hygeia Sciences, Inc. Immunoassay including lyophilized reactant mixture
US6352862B1 (en) * 1989-02-17 2002-03-05 Unilever Patent Holdings B.V. Analytical test device for imuno assays and methods of using same
IE68446B1 (en) * 1989-07-19 1996-06-12 Noctech Technology Limited A colour stable chromogen composition
US5176999A (en) * 1989-12-07 1993-01-05 Eastman Kodak Company Buffered wash composition, insolubilizing composition, test kits and method of use
DE4005630A1 (de) * 1990-02-22 1991-09-05 Behringwerke Ag Monoklonale antikoerper gegen tumorassoziierte antigene, verfahren zu ihrer herstellung sowie ihre verwendung
US5225325A (en) * 1990-03-02 1993-07-06 Ventana Medical Systems, Inc. Immunohistochemical staining method and reagents therefor
US6699685B1 (en) 1990-04-20 2004-03-02 Rcr Scientific, Inc. Method, test media and chromogenic compounds for identifying and differentiating general coliforms and escherichia coli bacteria
US5210022A (en) * 1990-04-20 1993-05-11 Rcr Scientific, Inc. Method test media and chromogenic compounds for identifying and differentiating general coliforms and Escherichia coli bacteria
US5532138A (en) * 1990-04-26 1996-07-02 Behringwerke Ag Method and kits for determining peroxidatively active catalysts
US5215885A (en) * 1990-10-23 1993-06-01 Beckman Instruments, Inc. Stable two-part chromogen substrate
US5238817A (en) * 1991-07-12 1993-08-24 E. I. Du Pont De Nemours And Company Chromogenic substrates for improving detection in a peroxidase-based assay
AU5407994A (en) * 1992-10-30 1994-05-24 Cetac Technologies Incorporated Method for particulate reagent sample treatment
US5384265A (en) * 1993-03-26 1995-01-24 Geo-Centers, Inc. Biomolecules bound to catalytic inorganic particles, immunoassays using the same
US5637508A (en) * 1993-03-26 1997-06-10 Geo-Centers, Inc. Biomolecules bound to polymer or copolymer coated catalytic inorganic particles, immunoassays using the same and kits containing the same
US5891657A (en) * 1994-05-19 1999-04-06 Strategic Diagnostics Inc. Immunoassay standards for volatile analytes with benzene rings
EP0714025A1 (en) * 1994-11-25 1996-05-29 Kyoto Dai-ichi Kagaku Co., Ltd. Method of and apparatus for determining hydrogen peroxide by Raman scattering
US20010051350A1 (en) 1995-05-02 2001-12-13 Albert Nazareth Diagnostic detection device and method
US5792618A (en) * 1996-09-27 1998-08-11 Moss, Inc. Liquid single-component substrates for detection or assay of horseradish peroxidase
EP0999447A4 (en) * 1997-04-30 2002-02-27 Maruha Corp METHOD FOR DETECTION OR PREDICTION OF ISCHEMIC DISEASES
AU8334698A (en) 1997-07-15 1999-02-10 Kem-En-Tec A/S Pre-stained 3,3',5,5'-tetramethylbenzidine substrates for the detection of enzyme activity
AU2493499A (en) * 1998-02-03 1999-08-16 Trustees Of Columbia University In The City Of New York, The Determination of the amount of hlhbeta core fragment in a sample from a subject and uses thereof
US6913929B1 (en) * 2000-04-18 2005-07-05 Pearl Technology Holdings, Llc Sexual fidelity and sex crime verification
US20030232340A1 (en) * 2002-06-13 2003-12-18 David Anderson Nanoporous particle with a retained target
US20040219620A1 (en) * 2003-04-29 2004-11-04 Mayer Brent A. Chromogenic substrate with a pH indicator dye
WO2004099384A2 (en) * 2003-05-02 2004-11-18 Sigma-Aldrich Co. Solid phase cell lysis and capture platform
US20080038843A1 (en) * 2006-08-11 2008-02-14 University Of Maryland - Baltimore Method of diagnosing celiac disease
WO2006026248A1 (en) 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
JP4824581B2 (ja) * 2005-01-07 2011-11-30 ヤマサ醤油株式会社 安定化されたオルニチントランスカルバミラーゼ及びそれを用いたオルニチントランスカルバミラーゼの免疫学的測定法
SG11201400677PA (en) 2011-09-16 2014-06-27 Credo Biomedical Pte Ltd Molecular diagnostic assay device and method of use
CN104569441B (zh) * 2015-01-19 2016-02-03 成都大熊猫繁育研究基地 大熊猫尿液促黄体素的酶联检测方法及其应用
US9927443B2 (en) 2015-04-10 2018-03-27 Conquerab Inc. Risk assessment for therapeutic drugs
US9903866B2 (en) 2016-04-05 2018-02-27 Conquerab Inc. Portable devices for detection of antibodies against therapeutic drugs
WO2019175745A1 (en) * 2018-03-13 2019-09-19 Kunaparaju, Venkata Subba Raju Methods and composition for preparing viscous substrate
CN110702897A (zh) * 2019-09-20 2020-01-17 北京贝尔生物工程股份有限公司 一种稳定高效的hrp酶促底物液及其制备方法与应用
CN111665241B (zh) * 2020-06-12 2023-03-28 苏州良辰生物仪器试剂有限公司 一种酪氨酸检测试纸条及其制备方法和应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL154600B (nl) * 1971-02-10 1977-09-15 Organon Nv Werkwijze voor het aantonen en bepalen van specifiek bindende eiwitten en hun corresponderende bindbare stoffen.
DE2460903C3 (de) * 1974-12-21 1981-12-24 Boehringer Mannheim Gmbh, 6800 Mannheim Neue 3,3',5,5'-Tetraalkylbenzidine
IL48741A (en) * 1975-12-26 1979-07-25 Rafa Labor Ltd Method for the determination of pregnancy
US4340395A (en) * 1979-11-13 1982-07-20 Miles Laboratories, Inc. Stabilization of benzidine-type indicators with various enhancers
NL8001972A (nl) * 1980-04-03 1981-11-02 Akzo Nv Verbeterde werkwijze voor het aantonen en bepalen van immuumperoxidase aktiviteit.
JPS57136165A (en) * 1981-02-18 1982-08-23 Mochida Pharmaceut Co Ltd Immunological measuring reagent
US4361648A (en) * 1981-08-13 1982-11-30 Miles Laboratories, Inc. Color fixed chromogenic analytical element
US4503143A (en) * 1982-08-20 1985-03-05 Btc Diagnostics Limited Partnership Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen
DE3311888C1 (de) * 1983-03-31 1984-11-22 Byk-Mallinckrodt Chemische Produkte Gmbh, 6057 Dietzenbach Verfahren zur Gewinnung einheitlicher Antikörper-Meerrettichperoxidase-Konjugate, die bei dem Verfahren gewonnenen Produkte und ihre Verwendung

Also Published As

Publication number Publication date
WO1986004421A1 (en) 1986-07-31
EP0207936A4 (en) 1989-04-24
US4503143A (en) 1985-03-05
DE3590722T1 ( ) 1987-11-19
EP0207936A1 (en) 1987-01-14

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