JPS6257306B2 - - Google Patents
Info
- Publication number
- JPS6257306B2 JPS6257306B2 JP58149378A JP14937883A JPS6257306B2 JP S6257306 B2 JPS6257306 B2 JP S6257306B2 JP 58149378 A JP58149378 A JP 58149378A JP 14937883 A JP14937883 A JP 14937883A JP S6257306 B2 JPS6257306 B2 JP S6257306B2
- Authority
- JP
- Japan
- Prior art keywords
- seaweed
- enzyme solution
- hydrolase
- protoplasts
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 241001474374 Blennius Species 0.000 claims description 63
- 102000004190 Enzymes Human genes 0.000 claims description 45
- 108090000790 Enzymes Proteins 0.000 claims description 45
- 229940088598 enzyme Drugs 0.000 claims description 45
- 210000001938 protoplast Anatomy 0.000 claims description 34
- 238000000034 method Methods 0.000 claims description 21
- 108090000604 Hydrolases Proteins 0.000 claims description 20
- 102000004157 Hydrolases Human genes 0.000 claims description 20
- 229920001282 polysaccharide Polymers 0.000 claims description 20
- 239000005017 polysaccharide Substances 0.000 claims description 20
- 150000004804 polysaccharides Chemical class 0.000 claims description 20
- 239000004365 Protease Substances 0.000 claims description 15
- 229920001221 xylan Polymers 0.000 claims description 15
- 229920000057 Mannan Polymers 0.000 claims description 13
- 108090000526 Papain Proteins 0.000 claims description 9
- 235000019834 papain Nutrition 0.000 claims description 9
- 229940055729 papain Drugs 0.000 claims description 9
- 244000005700 microbiome Species 0.000 claims description 7
- 108091005804 Peptidases Proteins 0.000 claims description 6
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 6
- 239000000411 inducer Substances 0.000 claims description 6
- 235000019419 proteases Nutrition 0.000 claims description 6
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- 239000001888 Peptone Substances 0.000 claims description 4
- 108010080698 Peptones Proteins 0.000 claims description 4
- 241000589516 Pseudomonas Species 0.000 claims description 4
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 235000019319 peptone Nutrition 0.000 claims description 4
- 238000003809 water extraction Methods 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 239000000243 solution Substances 0.000 description 38
- 239000002609 medium Substances 0.000 description 13
- 230000007910 cell fusion Effects 0.000 description 10
- 150000004823 xylans Chemical class 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 7
- 239000013535 sea water Substances 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 241000206607 Porphyra umbilicalis Species 0.000 description 4
- 241000589774 Pseudomonas sp. Species 0.000 description 4
- 241000206608 Pyropia tenera Species 0.000 description 4
- 238000005273 aeration Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000005520 cutting process Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002344 surface layer Substances 0.000 description 3
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000013028 medium composition Substances 0.000 description 2
- WVJKHCGMRZGIJH-UHFFFAOYSA-N methanetriamine Chemical compound NC(N)N WVJKHCGMRZGIJH-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 240000006240 Linum usitatissimum Species 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 229920001715 Porphyran Polymers 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000003163 cell fusion method Methods 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 210000001724 microfibril Anatomy 0.000 description 1
- 108700005457 microfibrillar Proteins 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000001052 yellow pigment Substances 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Seaweed (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58149378A JPS6041485A (ja) | 1983-08-16 | 1983-08-16 | 海苔のプロトプラストの調製法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58149378A JPS6041485A (ja) | 1983-08-16 | 1983-08-16 | 海苔のプロトプラストの調製法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6041485A JPS6041485A (ja) | 1985-03-05 |
| JPS6257306B2 true JPS6257306B2 (US06272168-20010807-M00014.png) | 1987-11-30 |
Family
ID=15473820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58149378A Granted JPS6041485A (ja) | 1983-08-16 | 1983-08-16 | 海苔のプロトプラストの調製法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6041485A (US06272168-20010807-M00014.png) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02117607U (US06272168-20010807-M00014.png) * | 1989-03-07 | 1990-09-20 |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0654071B2 (ja) * | 1989-11-25 | 1994-07-20 | 立山アルミニウム工業株式会社 | 組合せ出窓装置 |
| JP2008125422A (ja) * | 2006-11-20 | 2008-06-05 | Mie Prefecture | 海苔の単細胞化方法及び養殖方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5421427A (en) * | 1977-07-18 | 1979-02-17 | Mitsubishi Electric Corp | Unsaturated polyester varinish composition with low odor |
-
1983
- 1983-08-16 JP JP58149378A patent/JPS6041485A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5421427A (en) * | 1977-07-18 | 1979-02-17 | Mitsubishi Electric Corp | Unsaturated polyester varinish composition with low odor |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02117607U (US06272168-20010807-M00014.png) * | 1989-03-07 | 1990-09-20 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6041485A (ja) | 1985-03-05 |
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