JPS6215196B2 - - Google Patents
Info
- Publication number
- JPS6215196B2 JPS6215196B2 JP16579878A JP16579878A JPS6215196B2 JP S6215196 B2 JPS6215196 B2 JP S6215196B2 JP 16579878 A JP16579878 A JP 16579878A JP 16579878 A JP16579878 A JP 16579878A JP S6215196 B2 JPS6215196 B2 JP S6215196B2
- Authority
- JP
- Japan
- Prior art keywords
- urokinase
- ion
- polysaccharide granules
- crude
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 claims description 29
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 claims description 29
- 229960005356 urokinase Drugs 0.000 claims description 29
- 150000004676 glycans Chemical class 0.000 claims description 13
- 239000008187 granular material Substances 0.000 claims description 13
- 229920001282 polysaccharide Polymers 0.000 claims description 13
- 239000005017 polysaccharide Substances 0.000 claims description 13
- 239000007853 buffer solution Substances 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 5
- 102100039419 Plasminogen activator inhibitor 2 Human genes 0.000 claims description 4
- 239000002797 plasminogen activator inhibitor Substances 0.000 claims description 4
- 108010065822 urokinase inhibitor Proteins 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 238000011084 recovery Methods 0.000 claims 1
- 239000000126 substance Substances 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 238000005342 ion exchange Methods 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 238000001042 affinity chromatography Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000012535 impurity Substances 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 230000001698 pyrogenic effect Effects 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 239000002510 pyrogen Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 235000002639 sodium chloride Nutrition 0.000 description 3
- 238000005349 anion exchange Methods 0.000 description 2
- TZCXTZWJZNENPQ-UHFFFAOYSA-L barium sulfate Chemical compound [Ba+2].[O-]S([O-])(=O)=O TZCXTZWJZNENPQ-UHFFFAOYSA-L 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- QYPPJABKJHAVHS-UHFFFAOYSA-N Agmatine Natural products NCCCCNC(N)=N QYPPJABKJHAVHS-UHFFFAOYSA-N 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 239000001856 Ethyl cellulose Substances 0.000 description 1
- ZZSNKZQZMQGXPY-UHFFFAOYSA-N Ethyl cellulose Chemical compound CCOCC1OC(OC)C(OCC)C(OCC)C1OC1C(O)C(O)C(OC)C(CO)O1 ZZSNKZQZMQGXPY-UHFFFAOYSA-N 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- QYPPJABKJHAVHS-UHFFFAOYSA-P agmatinium(2+) Chemical compound NC(=[NH2+])NCCCC[NH3+] QYPPJABKJHAVHS-UHFFFAOYSA-P 0.000 description 1
- -1 aminobutylene guanidine Chemical compound 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-N anhydrous guanidine Natural products NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 229920001249 ethyl cellulose Polymers 0.000 description 1
- 235000019325 ethyl cellulose Nutrition 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 229940080469 phosphocellulose Drugs 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16579878A JPS5592687A (en) | 1978-12-28 | 1978-12-28 | Purification of urokinase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16579878A JPS5592687A (en) | 1978-12-28 | 1978-12-28 | Purification of urokinase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5592687A JPS5592687A (en) | 1980-07-14 |
| JPS6215196B2 true JPS6215196B2 (en:Method) | 1987-04-06 |
Family
ID=15819185
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16579878A Granted JPS5592687A (en) | 1978-12-28 | 1978-12-28 | Purification of urokinase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5592687A (en:Method) |
-
1978
- 1978-12-28 JP JP16579878A patent/JPS5592687A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5592687A (en) | 1980-07-14 |
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