JPS62118886A - β−ガラクトシダ−ゼの分離方法 - Google Patents
β−ガラクトシダ−ゼの分離方法Info
- Publication number
- JPS62118886A JPS62118886A JP25919985A JP25919985A JPS62118886A JP S62118886 A JPS62118886 A JP S62118886A JP 25919985 A JP25919985 A JP 25919985A JP 25919985 A JP25919985 A JP 25919985A JP S62118886 A JPS62118886 A JP S62118886A
- Authority
- JP
- Japan
- Prior art keywords
- galactosidase
- beta
- enzyme
- eluted
- phosphoric acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000005936 beta-Galactosidase Human genes 0.000 title claims abstract description 27
- 108010005774 beta-Galactosidase Proteins 0.000 title claims abstract description 27
- 238000000926 separation method Methods 0.000 title description 5
- 238000000034 method Methods 0.000 claims abstract description 15
- 229910052588 hydroxylapatite Inorganic materials 0.000 claims abstract description 10
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 claims abstract description 10
- 241000193752 Bacillus circulans Species 0.000 claims abstract description 7
- 229920001542 oligosaccharide Polymers 0.000 claims abstract description 7
- 150000002482 oligosaccharides Chemical class 0.000 claims abstract description 7
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 6
- 101710122007 Beta-galactosidase BgaB Proteins 0.000 claims abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract description 35
- 108090000790 Enzymes Proteins 0.000 abstract description 35
- 239000000126 substance Substances 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 3
- 108090000623 proteins and genes Proteins 0.000 abstract description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 8
- 229910000147 aluminium phosphate Inorganic materials 0.000 abstract 4
- 239000000872 buffer Substances 0.000 abstract 4
- 239000008363 phosphate buffer Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 10
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 238000010828 elution Methods 0.000 description 4
- 239000008101 lactose Substances 0.000 description 4
- 102000002464 Galactosidases Human genes 0.000 description 3
- 108010093031 Galactosidases Proteins 0.000 description 3
- KUWPCJHYPSUOFW-YBXAARCKSA-N 2-nitrophenyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CC=CC=C1[N+]([O-])=O KUWPCJHYPSUOFW-YBXAARCKSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000000108 ultra-filtration Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- IQUPABOKLQSFBK-UHFFFAOYSA-N 2-nitrophenol Chemical compound OC1=CC=CC=C1[N+]([O-])=O IQUPABOKLQSFBK-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101710195293 Beta-galactosidase 2 Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000235649 Kluyveromyces Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000008351 acetate buffer Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000011098 chromatofocusing Methods 0.000 description 1
- 238000004440 column chromatography Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021255 galacto-oligosaccharides Nutrition 0.000 description 1
- 150000003271 galactooligosaccharides Chemical class 0.000 description 1
- 150000008195 galaktosides Chemical class 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000006462 rearrangement reaction Methods 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25919985A JPS62118886A (ja) | 1985-11-18 | 1985-11-18 | β−ガラクトシダ−ゼの分離方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP25919985A JPS62118886A (ja) | 1985-11-18 | 1985-11-18 | β−ガラクトシダ−ゼの分離方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62118886A true JPS62118886A (ja) | 1987-05-30 |
| JPS6334B2 JPS6334B2 (enrdf_load_stackoverflow) | 1988-01-05 |
Family
ID=17330755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP25919985A Granted JPS62118886A (ja) | 1985-11-18 | 1985-11-18 | β−ガラクトシダ−ゼの分離方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS62118886A (enrdf_load_stackoverflow) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0530901A (ja) * | 1991-07-26 | 1993-02-09 | Ikaruga Giyuuniyuu:Kk | 低乳糖牛乳の製造方法 |
| JPH06105651A (ja) * | 1992-03-18 | 1994-04-19 | Ikaruga Giyuuniyuu:Kk | 低乳糖牛乳の製造方法 |
-
1985
- 1985-11-18 JP JP25919985A patent/JPS62118886A/ja active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0530901A (ja) * | 1991-07-26 | 1993-02-09 | Ikaruga Giyuuniyuu:Kk | 低乳糖牛乳の製造方法 |
| JPH06105651A (ja) * | 1992-03-18 | 1994-04-19 | Ikaruga Giyuuniyuu:Kk | 低乳糖牛乳の製造方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6334B2 (enrdf_load_stackoverflow) | 1988-01-05 |
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