JPS6131114B2 - - Google Patents
Info
- Publication number
- JPS6131114B2 JPS6131114B2 JP52145792A JP14579277A JPS6131114B2 JP S6131114 B2 JPS6131114 B2 JP S6131114B2 JP 52145792 A JP52145792 A JP 52145792A JP 14579277 A JP14579277 A JP 14579277A JP S6131114 B2 JPS6131114 B2 JP S6131114B2
- Authority
- JP
- Japan
- Prior art keywords
- group
- carbon atoms
- general formula
- chain
- straight
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 150000001875 compounds Chemical class 0.000 claims description 89
- 239000000203 mixture Substances 0.000 claims description 84
- 125000004432 carbon atom Chemical group C* 0.000 claims description 33
- 238000000034 method Methods 0.000 claims description 22
- 231100000252 nontoxic Toxicity 0.000 claims description 20
- 230000003000 nontoxic effect Effects 0.000 claims description 20
- 150000003839 salts Chemical class 0.000 claims description 18
- 239000002253 acid Substances 0.000 claims description 14
- 125000002947 alkylene group Chemical group 0.000 claims description 14
- 125000000217 alkyl group Chemical group 0.000 claims description 12
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 125000004430 oxygen atom Chemical group O* 0.000 claims description 10
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 8
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 claims description 8
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 claims description 7
- 125000000753 cycloalkyl group Chemical group 0.000 claims description 7
- 125000003884 phenylalkyl group Chemical group 0.000 claims description 7
- 125000001501 propionyl group Chemical group O=C([*])C([H])([H])C([H])([H])[H] 0.000 claims description 7
- 229920006395 saturated elastomer Polymers 0.000 claims description 7
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Chemical compound BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 claims description 6
- 239000003960 organic solvent Substances 0.000 claims description 6
- 230000003115 biocidal effect Effects 0.000 claims description 4
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims description 3
- 239000005977 Ethylene Substances 0.000 claims description 3
- 229910052794 bromium Inorganic materials 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 229910052801 chlorine Inorganic materials 0.000 claims description 3
- 125000001309 chloro group Chemical group Cl* 0.000 claims description 3
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 claims description 3
- 239000003937 drug carrier Substances 0.000 claims description 2
- 150000007529 inorganic bases Chemical class 0.000 claims description 2
- 150000003512 tertiary amines Chemical group 0.000 claims description 2
- 125000003258 trimethylene group Chemical group [H]C([H])([*:2])C([H])([H])C([H])([H])[*:1] 0.000 claims description 2
- 125000004429 atom Chemical group 0.000 claims 6
- ACTOXUHEUCPTEW-BWHGAVFKSA-N 2-[(4r,5s,6s,7r,9r,10r,11e,13e,16r)-6-[(2s,3r,4r,5s,6r)-5-[(2s,4r,5s,6s)-4,5-dihydroxy-4,6-dimethyloxan-2-yl]oxy-4-(dimethylamino)-3-hydroxy-6-methyloxan-2-yl]oxy-10-[(2s,5s,6r)-5-(dimethylamino)-6-methyloxan-2-yl]oxy-4-hydroxy-5-methoxy-9,16-dimethyl-2-o Chemical class O([C@H]1/C=C/C=C/C[C@@H](C)OC(=O)C[C@@H](O)[C@@H]([C@H]([C@@H](CC=O)C[C@H]1C)O[C@H]1[C@@H]([C@H]([C@H](O[C@@H]2O[C@@H](C)[C@H](O)[C@](C)(O)C2)[C@@H](C)O1)N(C)C)O)OC)[C@@H]1CC[C@H](N(C)C)[C@@H](C)O1 ACTOXUHEUCPTEW-BWHGAVFKSA-N 0.000 claims 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 93
- 239000000243 solution Substances 0.000 description 76
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 72
- ACTOXUHEUCPTEW-KWBWCIJSSA-N spiramycin I Chemical class O([C@H]1/C=C/C=C/C[C@@H](C)OC(=O)C[C@@H](O)[C@@H]([C@H]([C@@H](CC=O)C[C@H]1C)O[C@H]1[C@@H]([C@H]([C@H](O[C@@H]2O[C@@H](C)[C@H](O)[C@](C)(O)C2)[C@@H](C)O1)N(C)C)O)OC)[C@H]1CC[C@H](N(C)C)[C@H](C)O1 ACTOXUHEUCPTEW-KWBWCIJSSA-N 0.000 description 49
- 239000004187 Spiramycin Substances 0.000 description 48
- 229930191512 spiramycin Natural products 0.000 description 48
- 235000019372 spiramycin Nutrition 0.000 description 48
- 229960001294 spiramycin Drugs 0.000 description 47
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 45
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 34
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 33
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 33
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 30
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 27
- 239000007787 solid Substances 0.000 description 22
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 21
- 241001465754 Metazoa Species 0.000 description 17
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 17
- 235000017557 sodium bicarbonate Nutrition 0.000 description 17
- 238000003756 stirring Methods 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 17
- 239000004480 active ingredient Substances 0.000 description 16
- 238000002844 melting Methods 0.000 description 16
- 230000008018 melting Effects 0.000 description 16
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 15
- 235000019341 magnesium sulphate Nutrition 0.000 description 15
- 241000699670 Mus sp. Species 0.000 description 14
- 239000010410 layer Substances 0.000 description 14
- 239000002904 solvent Substances 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 208000015181 infectious disease Diseases 0.000 description 9
- 239000007788 liquid Substances 0.000 description 9
- 239000000080 wetting agent Substances 0.000 description 9
- 241000271566 Aves Species 0.000 description 8
- 230000037396 body weight Effects 0.000 description 7
- 235000008504 concentrate Nutrition 0.000 description 7
- 239000012141 concentrate Substances 0.000 description 7
- 239000002270 dispersing agent Substances 0.000 description 7
- 239000012259 ether extract Substances 0.000 description 7
- ZPCCSZFPOXBNDL-RSMXASMKSA-N spiramycin II Chemical compound O([C@H]1/C=C/C=C/C[C@@H](C)OC(=O)C[C@H]([C@@H]([C@H]([C@@H](CC=O)C[C@H]1C)O[C@H]1[C@@H]([C@H]([C@H](O[C@@H]2O[C@@H](C)[C@H](O)[C@](C)(O)C2)[C@@H](C)O1)N(C)C)O)OC)OC(C)=O)[C@H]1CC[C@H](N(C)C)[C@H](C)O1 ZPCCSZFPOXBNDL-RSMXASMKSA-N 0.000 description 7
- 229950006796 spiramycin ii Drugs 0.000 description 7
- 239000002585 base Substances 0.000 description 6
- 239000002026 chloroform extract Substances 0.000 description 6
- 239000008187 granular material Substances 0.000 description 6
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 6
- 239000013543 active substance Substances 0.000 description 5
- 239000003995 emulsifying agent Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000008247 solid mixture Substances 0.000 description 5
- 239000000375 suspending agent Substances 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 5
- -1 triethylamine Chemical class 0.000 description 5
- 229940126062 Compound A Drugs 0.000 description 4
- NLDMNSXOCDLTTB-UHFFFAOYSA-N Heterophylliin A Natural products O1C2COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC2C(OC(=O)C=2C=C(O)C(O)=C(O)C=2)C(O)C1OC(=O)C1=CC(O)=C(O)C(O)=C1 NLDMNSXOCDLTTB-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- RIFGWPKJUGCATF-UHFFFAOYSA-N ethyl chloroformate Chemical compound CCOC(Cl)=O RIFGWPKJUGCATF-UHFFFAOYSA-N 0.000 description 4
- 239000003701 inert diluent Substances 0.000 description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 description 4
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000010755 mineral Nutrition 0.000 description 4
- 239000011707 mineral Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 101000599862 Homo sapiens Intercellular adhesion molecule 3 Proteins 0.000 description 3
- 102100037871 Intercellular adhesion molecule 3 Human genes 0.000 description 3
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 239000006185 dispersion Substances 0.000 description 3
- 239000003651 drinking water Substances 0.000 description 3
- 235000020188 drinking water Nutrition 0.000 description 3
- 239000000839 emulsion Substances 0.000 description 3
- 150000002148 esters Chemical class 0.000 description 3
- 239000008188 pellet Substances 0.000 description 3
- 244000144977 poultry Species 0.000 description 3
- 235000013594 poultry meat Nutrition 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- WRMNZCZEMHIOCP-UHFFFAOYSA-N 2-phenylethanol Chemical compound OCCC1=CC=CC=C1 WRMNZCZEMHIOCP-UHFFFAOYSA-N 0.000 description 2
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 2
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241000286209 Phasianidae Species 0.000 description 2
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 241000191940 Staphylococcus Species 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- DKGAVHZHDRPRBM-UHFFFAOYSA-N Tert-Butanol Chemical compound CC(C)(C)O DKGAVHZHDRPRBM-UHFFFAOYSA-N 0.000 description 2
- 239000002671 adjuvant Substances 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 150000001450 anions Chemical class 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000000969 carrier Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 229960004419 dimethyl fumarate Drugs 0.000 description 2
- IXZFDJXHLQQSGQ-UHFFFAOYSA-N ethyl 4-chloro-4-oxobutanoate Chemical compound CCOC(=O)CCC(Cl)=O IXZFDJXHLQQSGQ-UHFFFAOYSA-N 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- 230000004151 fermentation Effects 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 235000019359 magnesium stearate Nutrition 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 230000000069 prophylactic effect Effects 0.000 description 2
- 239000001384 succinic acid Substances 0.000 description 2
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- FALRKNHUBBKYCC-UHFFFAOYSA-N 2-(chloromethyl)pyridine-3-carbonitrile Chemical compound ClCC1=NC=CC=C1C#N FALRKNHUBBKYCC-UHFFFAOYSA-N 0.000 description 1
- XISIEQIDFFXZCP-UHFFFAOYSA-N 4-oxo-4-phenoxybutanoic acid Chemical compound OC(=O)CCC(=O)OC1=CC=CC=C1 XISIEQIDFFXZCP-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-M Butyrate Chemical compound CCCC([O-])=O FERIUCNNQQJTOY-UHFFFAOYSA-M 0.000 description 1
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Natural products CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 1
- LVGKNOAMLMIIKO-UHFFFAOYSA-N Elaidinsaeure-aethylester Natural products CCCCCCCCC=CCCCCCCCC(=O)OCC LVGKNOAMLMIIKO-UHFFFAOYSA-N 0.000 description 1
- 241001522878 Escherichia coli B Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 101000993347 Gallus gallus Ciliary neurotrophic factor Proteins 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- 241000204022 Mycoplasma gallisepticum Species 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- XBDQKXXYIPTUBI-UHFFFAOYSA-M Propionate Chemical compound CCC([O-])=O XBDQKXXYIPTUBI-UHFFFAOYSA-M 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000187758 Streptomyces ambofaciens Species 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 108010021119 Trichosanthin Proteins 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 229910000288 alkali metal carbonate Inorganic materials 0.000 description 1
- 150000008041 alkali metal carbonates Chemical class 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 230000000507 anthelmentic effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 229940124536 anticoccidial agent Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003125 aqueous solvent Substances 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 235000004251 balanced diet Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 235000010216 calcium carbonate Nutrition 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001735 carboxylic acids Chemical class 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000003224 coccidiostatic agent Substances 0.000 description 1
- 239000008119 colloidal silica Substances 0.000 description 1
- JNGZXGGOCLZBFB-IVCQMTBJSA-N compound E Chemical compound N([C@@H](C)C(=O)N[C@@H]1C(N(C)C2=CC=CC=C2C(C=2C=CC=CC=2)=N1)=O)C(=O)CC1=CC(F)=CC(F)=C1 JNGZXGGOCLZBFB-IVCQMTBJSA-N 0.000 description 1
- 239000007891 compressed tablet Substances 0.000 description 1
- 239000007859 condensation product Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- HESULMMDQNKLFF-UHFFFAOYSA-N cyclohexyl 4-chloro-4-oxobutanoate Chemical compound ClC(=O)CCC(=O)OC1CCCCC1 HESULMMDQNKLFF-UHFFFAOYSA-N 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- KWFADUNOPOSMIJ-UHFFFAOYSA-N ethyl 3-chloro-3-oxopropanoate Chemical compound CCOC(=O)CC(Cl)=O KWFADUNOPOSMIJ-UHFFFAOYSA-N 0.000 description 1
- LVGKNOAMLMIIKO-QXMHVHEDSA-N ethyl oleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC LVGKNOAMLMIIKO-QXMHVHEDSA-N 0.000 description 1
- 229940093471 ethyl oleate Drugs 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000013355 food flavoring agent Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000001640 fractional crystallisation Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000008273 gelatin Substances 0.000 description 1
- 229920000159 gelatin Polymers 0.000 description 1
- 235000019322 gelatine Nutrition 0.000 description 1
- 235000011852 gelatine desserts Nutrition 0.000 description 1
- FVIZARNDLVOMSU-UHFFFAOYSA-N ginsenoside K Natural products C1CC(C2(CCC3C(C)(C)C(O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC1OC(CO)C(O)C(O)C1O FVIZARNDLVOMSU-UHFFFAOYSA-N 0.000 description 1
- ZTQSADJAYQOCDD-UHFFFAOYSA-N ginsenoside-Rd2 Natural products C1CC(C2(CCC3C(C)(C)C(OC4C(C(O)C(O)C(CO)O4)O)CCC3(C)C2CC2O)C)(C)C2C1C(C)(CCC=C(C)C)OC(C(C(O)C1O)O)OC1COC1OCC(O)C(O)C1O ZTQSADJAYQOCDD-UHFFFAOYSA-N 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000007952 growth promoter Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 125000000623 heterocyclic group Chemical group 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 229910000041 hydrogen chloride Inorganic materials 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002198 insoluble material Substances 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- WRCFPABNOIMFKO-UHFFFAOYSA-N methyl 4-chloro-3-methyl-4-oxobutanoate Chemical compound COC(=O)CC(C)C(Cl)=O WRCFPABNOIMFKO-UHFFFAOYSA-N 0.000 description 1
- SRXOJMOGPYFZKC-UHFFFAOYSA-N methyl 4-chloro-4-oxobutanoate Chemical compound COC(=O)CCC(Cl)=O SRXOJMOGPYFZKC-UHFFFAOYSA-N 0.000 description 1
- JCAZSWWHFJVFPP-UHFFFAOYSA-N methyl 5-chloro-5-oxopentanoate Chemical compound COC(=O)CCCC(Cl)=O JCAZSWWHFJVFPP-UHFFFAOYSA-N 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- KVBGVZZKJNLNJU-UHFFFAOYSA-N naphthalene-2-sulfonic acid Chemical compound C1=CC=CC2=CC(S(=O)(=O)O)=CC=C21 KVBGVZZKJNLNJU-UHFFFAOYSA-N 0.000 description 1
- 239000012457 nonaqueous media Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000020939 nutritional additive Nutrition 0.000 description 1
- 239000004006 olive oil Substances 0.000 description 1
- 235000008390 olive oil Nutrition 0.000 description 1
- 150000007530 organic bases Chemical class 0.000 description 1
- 150000002895 organic esters Chemical class 0.000 description 1
- 239000012044 organic layer Substances 0.000 description 1
- 235000006408 oxalic acid Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000007911 parenteral administration Methods 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 201000006509 pleuropneumonia Diseases 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 229920001592 potato starch Polymers 0.000 description 1
- 235000014483 powder concentrate Nutrition 0.000 description 1
- HDCOUCOKHNGTPH-UHFFFAOYSA-N propan-2-yl 4-chloro-4-oxobutanoate Chemical compound CC(C)OC(=O)CCC(Cl)=O HDCOUCOKHNGTPH-UHFFFAOYSA-N 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- LVTJOONKWUXEFR-FZRMHRINSA-N protoneodioscin Natural products O(C[C@@H](CC[C@]1(O)[C@H](C)[C@@H]2[C@]3(C)[C@H]([C@H]4[C@@H]([C@]5(C)C(=CC4)C[C@@H](O[C@@H]4[C@H](O[C@H]6[C@@H](O)[C@@H](O)[C@@H](O)[C@H](C)O6)[C@@H](O)[C@H](O[C@H]6[C@@H](O)[C@@H](O)[C@@H](O)[C@H](C)O6)[C@H](CO)O4)CC5)CC3)C[C@@H]2O1)C)[C@H]1[C@H](O)[C@H](O)[C@H](O)[C@@H](CO)O1 LVTJOONKWUXEFR-FZRMHRINSA-N 0.000 description 1
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
- 239000012429 reaction media Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 239000012047 saturated solution Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 201000009890 sinusitis Diseases 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 1
- 235000012424 soybean oil Nutrition 0.000 description 1
- 239000003549 soybean oil Substances 0.000 description 1
- ACTOXUHEUCPTEW-ZOTSFZJCSA-N spiramycin I Natural products CO[C@H]1[C@H](O)CC(=O)O[C@H](C)CC=CC=C[C@H](O[C@H]2CC[C@@H]([C@@H](C)O2)N(C)C)[C@H](C)C[C@H](CC=O)[C@@H]1O[C@@H]3O[C@H](C)[C@@H](O[C@H]4C[C@@](C)(O)[C@@H](O)[C@H](C)O4)[C@@H]([C@H]3O)N(C)C ACTOXUHEUCPTEW-ZOTSFZJCSA-N 0.000 description 1
- ACTOXUHEUCPTEW-UHFFFAOYSA-N spiramycins Chemical class CC1CC(CC=O)C(OC2C(C(C(OC3OC(C)C(O)C(C)(O)C3)C(C)O2)N(C)C)O)C(OC)C(O)CC(=O)OC(C)CC=CC=CC1OC1CCC(N(C)C)C(C)O1 ACTOXUHEUCPTEW-UHFFFAOYSA-N 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229940014800 succinic anhydride Drugs 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000000829 suppository Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 235000012222 talc Nutrition 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 238000011287 therapeutic dose Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 239000008158 vegetable oil Substances 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
- C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
- C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Animal Behavior & Ethology (AREA)
- Oncology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Communicable Diseases (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Saccharide Compounds (AREA)
Description
æ¬çºæã¯ãæ°èŠãªæçç©è³ªããããã®è£œæ³ãã
ã³ããããå«æããçµæç©ã«é¢ããã æ¬çºæã«ããã°ãäžè¬åŒ ãåŒäžãR1ã¯ã¢ã»ãã«åºãŸãã¯ããããªãã«åºã
ããã¯å¥œãŸããã¯æ°ŽçŽ ååãè¡šãããã¯ãã¿ãž
ãšãã¬ã³åºïŒïŒ¡äžã«ååšããäºéçµåã¯ãã©ã³ã¹
ã§ããïŒãè¡šããããããŠR2ã¯äžè¬åŒ ãåŒäžãã¯ïŒãïŒåã®ççŽ ååãæããïŒäŸ¡ã®
飜åãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢ã«ãã¬
ã³åºïŒäŸãã°ãšãã¬ã³ïŒãè¡šãããã¯ããããŸ
ãã¯å¥œãŸããã¯é žçŽ ååãè¡šããããããŠR3ã¯
ïŒãïŒå奜ãŸããã¯ïŒãïŒåã®ççŽ ååãæãã
çŽéãŸãã¯æåãéã®ã¢ã«ãã«åºãïŒãïŒåã®ç
çŽ ååãæããã·ã¯ãã¢ã«ãã«åºãããšãã«åºãŸ
ãã¯ããšãã«ã¢ã«ãã«åºïŒãã®ã¢ã«ãã¬ã³éšåã¯
çŽéãŸãã¯æåãéã§ãããããŠïŒãïŒåã®ççŽ
ååãæããïŒãè¡šãããã®æ°èŠãªã¹ãã©ãã€ã·
ã³èªå°äœããã³ãããã®æ··åç©ç¹ã«è©²æ··åç©äžã«
å«æãããäžè¬åŒã®ååç©ãR1ã«ã€ããŠã ã
ç°ãªããããŠïŒ¡ããã³R2ã«ã€ããŠåãã§ããæ··
åç©ãªãã³ã«ãããã®éæ¯æ§é žä»å å¡©ãæäŸãã
ãã äžè¬åŒã«ãããŠR2ãäžè¬åŒã®åºãè¡šã
ããäžè¬åŒã«ãããŠïŒºããšãã¬ã³ãŸãã¯ããªã¡
ãã¬ã³åºãè¡šããããé žçŽ ååãè¡šãããããŠ
R3ãïŒãïŒåã®ççŽ ååãæããçŽéã¢ã«ãã«
åºå¥œãŸããã¯ãšãã«ãè¡šããã¹ãã©ãã€ã·ã³èªå°
äœã奜é©ã§ããã ãéæ¯æ§é žä»å å¡©ããªãèªã¯ãå¡©ã®é°ã€ãªã³ã
æ²»ççæäžéã§çšããããå Žåã«åç©çµç¹ã«å¯Ÿã
ãŠæ¯èŒçç¡æ¯ã§ããããã®çµæéœã€ãªã³ã®æå©ãª
æ§è³ªãé°ã€ãªã³ã«èµ·å ããå¯äœçšã«ãã€ãŠç¡å¹ã«
ãããªããããªå¡©ãæå³ããã æ¬æ现æžã«ãããŠãäžè¬åŒã®ååç©ã«èšåã
ããå Žåã¯ãã€ã§ããåèšã®å¡©ã«ãèšåãããã
ãšãæå³ãããã åœæ¥è ã«ãšã€ãŠæãããªããã«ãäžè¬åŒã®åº
ã¯ïŒºããã³R3ã®éšåã®äžæ¹ãŸãã¯äž¡æ¹ã«ããã
ãã©ãªãã€ã®çµæãšããŠãã©ãªãã€ãè¡šãããŠã
ãããããç¥ãããŠããããã«ããã©ãªãã€ã®å
åšã¯ç°æ§äœãçããå¯èœæ§ããããæ¬çºæã¯ãã®
ç¯å²å ã«äžè¬åŒã®åºã«ãããåèšã®ãã©ãªãã€
ããçããäžè¬åŒã®ãã¹ãŠã®ç°æ§äœããã³ãã
ãã®æ··åç©ãå å«ããã æ¬çºæã®ç¹åŸŽã«ããã°ãR1ãããã³R2ãå
ã«å®çŸ©ããéãã§ããäžè¬åŒã®ååç©ã¯ãã«ã«
ãã³é žãããšã¹ãã«ã補é ããããã®æ¢ç¥ã®æ¹æ³
ãé©çšãŸãã¯æ¡çšããããšã«ãã€ãŠãäŸãã°äžè¬
åŒ R3YH ïŒïŒ ïŒåŒäžãããã³R3ã¯å ã«å®çŸ©ããéãã§ããïŒ
ã®ååç©ãšåå¿ãããããšã«ãã€ãŠãäžè¬åŒ ãåŒäžãR1ããã³ïŒ¡ã¯å ã«å®çŸ©ããéãã§ããã
ãããŠR4ã¯äžè¬åŒ ïŒåŒäžãã¯å ã«å®çŸ©ããéãã§ããïŒã®åºãè¡š
ãããã®ååç©ãã補é ãããã åèšã®åå¿ã¯äžè¬åŒ X1COOR5 ïŒåŒäžãX1ã¯èçŽ ååãŸãã¯å¡©çŽ ååãè¡šããã
ããŠR5ã¯ïŒãïŒåã®ççŽ ååãæããã¢ã«ãã«
åºãè¡šããïŒã®ååç©ããã³é žå容äœäŸãã°ç¬¬ïŒ
ã¢ãã³äŸãã°ããªãšãã«ã¢ãã³ã®ååšäžã«å¥œãŸã
ãã¯â30âãªããïŒ30âã®æž©åºŠã§ããŸã奜ãŸãã
ã¯ç¡æ°ŽäžæŽ»æ§ææ©æº¶å€äŸãã°ã¯ãããã«ã ã®ååš
äžã«è¡ãããã ããã«æ¬çºæã®ç¹åŸŽã«ããã°ãR1ãããã³
R2ãå ã«å®çŸ©ããéãã§ããäžè¬åŒã®ååç©
ã¯ãã¢ã«ã³ãŒã«ãããšã¹ãã«ã補é ããããã®æ¢
ç¥ã®æ¹æ³ãé©çšãŸãã¯æ¡çšããããšã«ãã€ãŠãäŸ
ãã°äžè¬åŒ X2OCZCOYR3 ãåŒäžãX2ã¯èçŽ ååãŸãã¯å¡©çŽ ååãããã¯äž
è¬åŒ âOCOOR5 ïŒåŒäžãR5ã¯å ã«å®çŸ©ããéãã§ããïŒã®åºãè¡š
ããããããŠïŒºãããã³R3ã¯å ã«å®çŸ©ããé
ãã§ãããã®ååç©ãšåå¿ãããããšã«ãã€ãŠã
äžè¬åŒ ïŒR1ããã³ïŒ¡ã¯å ã«å®çŸ©ããéãã§ããïŒã®åå
ç©ãã補é ãããã åèšã®åå¿ã¯ãå Žåã«ããé žå容äœäŸãã°ç¬¬ïŒ
ã¢ãã³äŸãã°ããªãšãã«ã¢ãã³ãŸãã¯ç¡æ©å¡©åºäŸ
ãã°éçé žãããªãŠã ã®ååšäžã«ã奜ãŸããã¯ç¡
æ°ŽäžæŽ»æ§ææ©æº¶å€äŸãã°ã¯ãããã«ã ãŸãã¯ã¢ã»
ãã³ã®ååšäžã«ãããŠå¥œãŸããã¯â30âãªããïŒ
30âã®æž©åºŠã§è¡ãªãããã äžè¬åŒã®ååç©ã®éæ¯æ§é žä»å å¡©ã¯ææ©å¡©åº
ã®å¡©ã®è£œé ã®ããã«ç¥ãããæ¹æ³ãé©çšãŸãã¯æ¡
çšããããšã«ãã€ãŠãäŸãã°äžè¬åŒã®ååç©ã
é©çšãªæº¶å€äžã®é©åœãªé žãšåå¿ãããããšã«ãã€
ãŠè£œé ããŠããããå¡©ã¯æº¶æ¶²ã®èŠªæ¶²æ§åïŒãªãªã
ã€ãªãŒãŒã·ãšã³ïŒã«ãã€ãŠããããã¯åå¿åªäœäž
ã«åå溶解ãªããã°å¿ èŠã«ãã溶å€ã®äžéšãé€å»
ããåŸéã«ãã€ãŠåé¢ããŠãããã äžè¬åŒã®ååç©ã®æ··åç©ã¯ãæèäœçšæ··åç©
ã®åé¢ã®ããã®æ¢ç¥æ¹æ³ãé©çšãŸãã¯æ¡çšããã
ãšã«ãã€ãŠãäŸãã°ã¯ã¬ã€ã°ïŒCraigïŒè£ 眮ïŒãš
ãŒã»ãŠãšã€ã¹ãã«ã¬ãŒæ°èããã¯ããã¯ã»ãªãã»
ãªãŒã¬ããã¯ã»ã±ãã¹ããªã€ã第ïŒå·»ç¬¬286é ïŒ
ã§åæµååžæ³ã«ãã€ãŠãã¯ãããã°ã©ãã€ãŒïŒäŸ
ãã°ã¢ã«ãããŸãã¯ã·ãªã«ã²ã«äžã§ïŒã«ãã€ãŠã
ããã¯äŸãã°è³éŠæçåæ°ŽçŽ æº¶å€äŸãã°ãã³ãŒã³
ããã®åå¥çµæ¶ã«ãã€ãŠãäžè¬åŒã®æåååç©
ã«åé¢ããŠãããã äžè¬åŒã®ååç©ã¯ãžã«ã«ãã³é žãããšã¹ãã«
ã補é ããããã«ç¥ãããæ¹æ³ãé©çšãŸãã¯æ¡çš
ããããšã«ãã€ãŠãäŸãã°äžè¬åŒ ïŒåŒäžãã¯å ã«å®çŸ©ããéãã§ããïŒã®ååç©
ãšåå¿ãããããšã«ãã€ãŠãäžè¬åŒã®ååç©ã
ã補é ããŠãããã åèšã®åå¿ã¯äžæŽ»æ§ææ©æº¶å€ã®ååšäžã«å¥œãŸã
ãã¯å®€æž©ã«ãããŠå Žåã«ããå¡©åºäŸãã°ã¢ã«ã«ãª
éå±çé žå¡©ãŸãã¯éçé žå¡©ã第ïŒã¢ãã³ãããã¯
è€çŽ ç°åŒç¬¬ïŒå¡©åºäŸãã°ããªãžã³ã®ååšäžã§è¡ã€
ãŠãããã äžè¬åŒã®ååç©ã¯ãã¢ã«ã³ãŒã«ããã³ããªã³
ãŒã«ãããšã¹ãã«ã補é ããããã®æ¢ç¥æ¹æ³ãé©
çšãŸãã¯æ¡çšããããšã«ãã€ãŠãäŸãã°äžè¬åŒ
ã®ååç©ããäžè¬åŒã®ååç©ã補é ããããã«
å ã«è¿°ã¹ãæ¹æ³ãšåæ§ã«ããŠäžè¬åŒã®ååç©ã
äžè¬åŒïŒžã®ååç©ãšåå¿ãã次ãã§ã«ã«ãã³é žã
ãããã²ã³åã¢ã·ã«ãŸãã¯æ··åé žç¡æ°Žç©ã補é ã
ãããã®æ¢ç¥æ¹æ³ãé©çšãŸãã¯æ¡çšããããšã«ã
ã€ãŠäžè¿°ã®ããã«ããŠåŸããããããšã¹ãã«ãäž
è¬åŒã®ååç©ã«å€æããããšã«ãã€ãŠãäžè¬åŒ
ã®ååç©ãã補é ããŠãããã äžè¬åŒã«ãããŠR1ãæ°ŽçŽ ååãã¢ã»ãã«åº
ãŸãã¯ããããªãã«åºãè¡šããååç©ãããªãã¡
ããããã¹ãã©ãã€ã·ã³ãã¹ãã©ãã€ã·ã³ã
ãã³ã¹ãã©ãã€ã·ã³ãšããŠç¥ãããååç©ãã
ã³ãããã®æ··åç©ã¯ãã¹ãã©ãã€ã·ã³ã®è£œé ã®ã
ãã®æ¢ç¥æ¹æ³ãé©çšãŸãã¯æ¡çšããããšã«ãã€
ãŠãäŸãã°ã¹ãã¬ãããã€ã»ã¹ã»ã¢ã ããã¢ã·ã€
ãšã³ã¹ïŒStreptomyces ambofaciensïŒ
NRRL2420ãŸãã¯ãã®ã¹ãã©ãã€ã·ã³çç£æ§çªç¶
å€ç°æ§ã®å¥œæ°æ§å¹é€ã«ãã€ãŠè£œé ããŠãããã ã¹ãã¬ãããã€ã»ã¹ã»ã¢ã ããã¢ã·ã€ãšã³ã¹ã®
çºé µã«ãã€ãŠæ®éã«åŸãããã¹ãã©ãã€ã·ã³ã¯ã
ãããïŒçš®ã®ã¹ãã©ãã€ã·ã³ã®æ··åç©ã§ãããã
ãŸããããã®ã¹ãã©ãã€ã·ã³ã®ããããç¹ã«ã¹ã
ã©ãã€ã·ã³ãçºé µã«ãã€ãŠçŽæ¥åŸãããšãå¯èœ
ã§ãããProd.Pharm.17(4)ãïŒïŒ1962ïŒåç §ãã æ¬æ现æžã§çšãããããããªãæ¢ç¥æ¹æ³ããªã
èªã¯ãããèªäœãããŸã§ã«çšããããæ¹æ³ããã
ã¯ååŠæç®ã«èšèŒãããæ¹æ³ãæå³ããã äžè¬åŒã®ååç©ããã³ãããã®éæ¯æ§é žä»å
å¡©ã¯ãã¹ã¿ãã€ãã³ãã«ã¹ã»ã¢ãŠã¬ãŠã¹
ïŒStaphylococcus aureusïŒãèºççèããã³é£é
çèã®ææã«èµ·å ããææã®æ²»çãªãã³ã«ãã³ã
ã©ãºãã»ã¬ãªã»ããã€ã«ã ïŒMycoplasma
gallisepticumïŒãšããŠç¥ãããèžèèºçæ§çç©
ã«ãã€ãŠæ¬æ¥ããããããäžé¢é³¥ã®ãã³ãã©ã¹ã¢
ã·ã¹ïŒæææ§éèæŽçãŸãã¯æ°å¢çãšããŠãç¥ã
ããçŸç ïŒã®æ²»çã«ãããåç©è¬ãšããŠæ¥µããŠæ
çšãªããããæèæ§ãæããæçç©è³ªã§ããã äžè¬åŒã®ååç©ããã³ãããã®éæ¯æ§é žä»å
å¡©ã¯ãŸããåç©ããã³é³¥ç¹ã«å®¶èããã³å®¶çŠœäŸã
ã°è±ãçäŸãã°ä»çããã³å®¶çŠœäŸãã°é¶ããã³äž
é¢é³¥ã®çé·çããã³ïŒãŸãã¯ïŒé£Œæå€ææ¯ã®æ¹è¯
ã«æ¥µããŠæçšãªãã®ãšãããçé·ä¿é²æŽ»æ§ãæã
ããåç©ãŸãã¯é³¥ã®çé·çã«ããããã®ãããªæ¹
è¯ã¯ãéåžžå¿ èŠãšããããããçãæéã«äŸãã°
åžå Žã®ããã«æãŸããäœéã«éããããããã¯å
ãæéã«ããã€ãŠããã«äœéãå¢å ããããšãæ
å³ãããåç©ããã³é³¥ã®é£Œæå€ææ¯ã«ããããã®
ãããªæ¹è¯ã¯ãäžè¬åŒã®ååç©ãŸãã¯ãã®éæ¯
æ§é žä»å å¡©ãäžããããªãåæ§ãªåç©ãŸãã¯é³¥ã
ããç¹å®ãªäœéã«éããã®ã«é£Œæãå°ãªããŠãã
ããšãæå³ããããŸãã家犜ã«æäžãããå Žåã
äžè¬åŒã®ååç©ã¯åµã®çç£ãä¿é²ããã®ã«æçš
ã§ãããäžè¬åŒã®ååç©ã¯å¹³è¡¡é£ãšäžç·ã«åç©
ããã³é³¥ã«æäžãããã®ãæ®éã§ããã äžè¬åŒã®ååç©ã¯äžè¬åŒã®ååç©ãšåæ§ãª
çš®é¡ããã³çšåºŠã®æ²»çããã³çé·ä¿é²æŽ»æ§ãæ
ãããããŠããã«ãããã¯äžè¬åŒã®ååç©ãã
èŠå³ãå°ããããã¯å®éã«ã¯ç¡å³ã§ãããšããé
èŠãªå©ç¹ãæããããã®å©ç¹ã¯äžè¬åŒã®ååç©
ã®çµå£æäžã«ãã幌å ãåç©ããã³é³¥ãæ²»çãã
å Žåã«ç¹ã«éèŠã§ããã äžè¬åŒã®ååç©ã®éæ¯æ§é žä»å å¡©ã¯äžè¬åŒ
ã®ååç©ããèŠå³ãå°ããšããå©ç¹ããªãããã
ããªããããããã¯åœæ¥è ã«ãšã€ãŠããç¥ããã
æè¡ã«ãã€ãŠãäŸãã°æ°Žäžããã³ææ©æº¶å€äžã®å¡©
ãšããšã®å¡©åºãšã®éã®æº¶è§£åºŠã®å·®ãå©çšããŠãäž
è¬åŒã®ååç©ã粟補ããå Žåã«æçšã§ãããäž
è¬åŒã®ããšã®å¡©åºã¯æ¢ç¥æ¹æ³ã«ãã€ãŠäŸãã°é±
ç©å¡©åºäŸãã°æ°Žæ§æ°Žé žåãããªãŠã å¡©åºãšåŠçã
ãããšã«ãã€ãŠããããã®å¡©ããåçããããšã
ã§ããã è±åœç¹èš±ç¬¬796311å·æ现æžã«ã¯ãèèªæç³»ã®ãž
ã«ã«ãã³é žãšã¹ãã©ãã€ã·ã³ã«ãã€ãŠçæããã
ãããšã¹ãã«ãé瀺ãããŠãããããã®ãããšã¹
ãã«ã¯äžè¬åŒã®ååç©ããèŠå³ãå°ãã ããã§ããäžè¬åŒã®ååç©ã¯åèšã®è±åœç¹èš±
第796311å·æ现æžã«é瀺ãããååç©ãããäžå±€
èŠå³ãå°ãã äžè¬åŒã®ä»¥äžã®ååç©ããã³ãããã®æ··åç©
ã¯ç¹ã«èå³ãããã ååç© ã¹ãã©ãã€ã·ã³ã®ä»¥äžã®ååç© ïŒ¡ 2â²âãšãã«ãµã¯ã·ããŒã  2â²âãšãã«ã°ã«ã¿ã¬ãŒã  2â²âã¡ãã«ãµã¯ã·ããŒã  2â²âãããã«ãµã¯ã·ããŒã  2â²âã¡ãã«ã°ã«ã¿ã¬ãŒã  2â²âã¡ãã«ããã¬ãŒã  2â²ââã¡ãã«ããªãµã¯ã·ããŒã  2â²âã·ã¯ãããã·ã«ãµã¯ã·ããŒã  2â²âãšãã«ããããŒã  2â²âããšãã«ãµã¯ã·ããŒã  2â²âã¡ãã«ïŒRSïŒâïŒïŒâã¡ãã«ïŒãµã¯ã·ã
ãŒã ïŒ 2â²âïŒïŒâããšãã«ãšãã«ïŒãµã¯ã·ããŒã  2â²â第ïŒããã«ãµã¯ã·ããŒã  2â²âã€ãœãããã«ãµã¯ã·ããŒã  ã¹ãã©ãã€ã·ã³æ··åç©ã®2â²âãšãã«ãµã¯ã·ã
ãŒãããã㳠 ååç©ïŒ¡ã®äºå¡©é žå¡©  ååç©ïŒ¡ã®äºãããé žå¡© åèšïŒ¡ãããã³ïŒªãã®æåã¯æ¬æ现æžäžäŸ
ãã°ä»¥äžã®è¡šã«ãããåç §ã容æã«ããããã«å
åç©ã«éãããã äžè¬åŒã®ååç©ã«é¢ããŠè¡ãªãããè©Šéšç®¡å
ã®æ²»ç掻æ§ãšçç©æŽ»æ§ã«é¢ããå®éšã§ã¯ã以äžã®
è©ŠéšçµæãåŸãããã è©ŠéšïŒ çµå£çã«åŠçãããããŠã¹ ã ãã³ïŒ0.5mlïŒäžã«æžæ¿ãããã¹ã¿ãã€ãã³
ãã«ã¹ã»ã¢ãŠã¬ãŠã¹ã¹ãã¹ïŒStaphylococcus
aureus SmithïŒãããŠã¹ã®è ¹è å ã«æ³šå°ããã
ãã®ææã¯100ïŒ ã®ããŠã¹ãæ»äº¡ãããããã«èš
ç®ãããæå°æäžéã®çŽ100åã瀺ãããããŠã¹
ã«äžè¬åŒã®ååç©ãåç©ã®äœéïŒKgåœãããã
ã16mgã31mgã62.5mgã125mgãŸãã¯250mgçµå£æ
äžãããææåŸïŒãïŒã24ã30ããã³48æéã«ã
æäžéãéžæããŠããããã®ããŠã¹ã«æäžããã
ææåŸæ°æ¥éããŠã¹ã芳å¯ããã äžè¬åŒã®ååç©ããåŸãããçµæã¯æšæºåå
ç©ãšããŠçšããããã¹ãã©ãã€ã·ã³ããåŸãã
ãçµæãšæ¯èŒãããã åŸãããçµæã¯ä»¥äžã®è¡š(ii)ã«ç€ºãããã ãCD50ããªãèªã¯ãåŠçãããããŠã¹ã®50ïŒ
ãæ²»çããããã«èšç®ãããæäžéãæå³ããã è©ŠéšïŒ ç®äžçã«åŠçãããããŠã¹ ã ãã³ïŒ0.5mlïŒäžã«æžæ¿ãããã¹ã¿ãã€ãã³
ãã«ã¹ã»ã¢ãŠã¬ãŠã¹ã¹ãã¹ãææãããããŠã¹
100ïŒ ãæ»ã«èŽãããæå°éã®çŽ100åãã§ããŠã¹
ã®è ¹è å ãææããããææåŸïŒãïŒã24ã30ã
ãã³48æéã«äžè¬åŒã®ååç©ãåç©ã®äœéïŒKg
åœã1.6mgã3.1mgã6.25mgã12.5mgãŸãã¯25mgã§
ããŠã¹ãããããç®äžçã«åŠçãããææåŸæ°æ¥
éããŠã¹ã芳å¯ããã äžè¬åŒã®ååç©ããåŸãããçµæãæšæºåå
ç©ãšããŠçšããããã¹ãã©ãã€ã·ã³ããåŸãã
ãçµæãšæ¯èŒãããããªãã¡ãååç©ïŒ¡ã¯åç©ã®
äœéïŒKgåœã17.5mgã®CD50ã瀺ããããã¹ãã©
ãã€ã·ã³ã¯åç©ã®äœéïŒKgåœã5.8mgã®CD50ã
瀺ããã è©ŠéšïŒ è©Šéšç®¡å æŽ»æ§ åè©Šéšååç©ã«å¯ŸããŠãããªããã³å€§è±æ±äžã®
ããç¯å²ã®æ¿åºŠã調補ãããç°ã€ãæ¿åºŠã®è©Šéšå
åç©ããã®ç¯å²å ã§å«æããããããã®è©Šéšç®¡ã«
ã¹ã¿ãã€ãã³ãã«ã¹ã»ã¢ãŠã¬ãŠã¹ïŒãªãã¯ã¹ããª
ãŒãïŒãŸãã¯ãšã³ãšãªãã¢ã»ã³ãªïŒ¢ïŒãšã³ãã¹ïŒ
ãEscherichia coli ïŒEnnisïŒãïŒè©Šéšç®¡ïŒæ¬åœ
ãçŽ105åã®åŸ®çç©ïŒããéžã°ããè©Šéšçç©ãæ¥
çš®ããããŠ37âã§ã€ã³ããŠããŒã·ãšã³ããåŸè©Šéš
çç©ã®çé·ãæå¶ããã®ã«å¿ èŠãšãããç¯å²å ã®
è©Šéšååç©ã®æäœæ¿åºŠããã®ååç©ãšãã®çç©ã«
察ããæå°æå¶æ¿åºŠãšããŠèšé²ããã åŸãããçµæã以äžã®è¡š(i)ã«èšèŒããã
ã³ããããå«æããçµæç©ã«é¢ããã æ¬çºæã«ããã°ãäžè¬åŒ ãåŒäžãR1ã¯ã¢ã»ãã«åºãŸãã¯ããããªãã«åºã
ããã¯å¥œãŸããã¯æ°ŽçŽ ååãè¡šãããã¯ãã¿ãž
ãšãã¬ã³åºïŒïŒ¡äžã«ååšããäºéçµåã¯ãã©ã³ã¹
ã§ããïŒãè¡šããããããŠR2ã¯äžè¬åŒ ãåŒäžãã¯ïŒãïŒåã®ççŽ ååãæããïŒäŸ¡ã®
飜åãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢ã«ãã¬
ã³åºïŒäŸãã°ãšãã¬ã³ïŒãè¡šãããã¯ããããŸ
ãã¯å¥œãŸããã¯é žçŽ ååãè¡šããããããŠR3ã¯
ïŒãïŒå奜ãŸããã¯ïŒãïŒåã®ççŽ ååãæãã
çŽéãŸãã¯æåãéã®ã¢ã«ãã«åºãïŒãïŒåã®ç
çŽ ååãæããã·ã¯ãã¢ã«ãã«åºãããšãã«åºãŸ
ãã¯ããšãã«ã¢ã«ãã«åºïŒãã®ã¢ã«ãã¬ã³éšåã¯
çŽéãŸãã¯æåãéã§ãããããŠïŒãïŒåã®ççŽ
ååãæããïŒãè¡šãããã®æ°èŠãªã¹ãã©ãã€ã·
ã³èªå°äœããã³ãããã®æ··åç©ç¹ã«è©²æ··åç©äžã«
å«æãããäžè¬åŒã®ååç©ãR1ã«ã€ããŠã ã
ç°ãªããããŠïŒ¡ããã³R2ã«ã€ããŠåãã§ããæ··
åç©ãªãã³ã«ãããã®éæ¯æ§é žä»å å¡©ãæäŸãã
ãã äžè¬åŒã«ãããŠR2ãäžè¬åŒã®åºãè¡šã
ããäžè¬åŒã«ãããŠïŒºããšãã¬ã³ãŸãã¯ããªã¡
ãã¬ã³åºãè¡šããããé žçŽ ååãè¡šãããããŠ
R3ãïŒãïŒåã®ççŽ ååãæããçŽéã¢ã«ãã«
åºå¥œãŸããã¯ãšãã«ãè¡šããã¹ãã©ãã€ã·ã³èªå°
äœã奜é©ã§ããã ãéæ¯æ§é žä»å å¡©ããªãèªã¯ãå¡©ã®é°ã€ãªã³ã
æ²»ççæäžéã§çšããããå Žåã«åç©çµç¹ã«å¯Ÿã
ãŠæ¯èŒçç¡æ¯ã§ããããã®çµæéœã€ãªã³ã®æå©ãª
æ§è³ªãé°ã€ãªã³ã«èµ·å ããå¯äœçšã«ãã€ãŠç¡å¹ã«
ãããªããããªå¡©ãæå³ããã æ¬æ现æžã«ãããŠãäžè¬åŒã®ååç©ã«èšåã
ããå Žåã¯ãã€ã§ããåèšã®å¡©ã«ãèšåãããã
ãšãæå³ãããã åœæ¥è ã«ãšã€ãŠæãããªããã«ãäžè¬åŒã®åº
ã¯ïŒºããã³R3ã®éšåã®äžæ¹ãŸãã¯äž¡æ¹ã«ããã
ãã©ãªãã€ã®çµæãšããŠãã©ãªãã€ãè¡šãããŠã
ãããããç¥ãããŠããããã«ããã©ãªãã€ã®å
åšã¯ç°æ§äœãçããå¯èœæ§ããããæ¬çºæã¯ãã®
ç¯å²å ã«äžè¬åŒã®åºã«ãããåèšã®ãã©ãªãã€
ããçããäžè¬åŒã®ãã¹ãŠã®ç°æ§äœããã³ãã
ãã®æ··åç©ãå å«ããã æ¬çºæã®ç¹åŸŽã«ããã°ãR1ãããã³R2ãå
ã«å®çŸ©ããéãã§ããäžè¬åŒã®ååç©ã¯ãã«ã«
ãã³é žãããšã¹ãã«ã補é ããããã®æ¢ç¥ã®æ¹æ³
ãé©çšãŸãã¯æ¡çšããããšã«ãã€ãŠãäŸãã°äžè¬
åŒ R3YH ïŒïŒ ïŒåŒäžãããã³R3ã¯å ã«å®çŸ©ããéãã§ããïŒ
ã®ååç©ãšåå¿ãããããšã«ãã€ãŠãäžè¬åŒ ãåŒäžãR1ããã³ïŒ¡ã¯å ã«å®çŸ©ããéãã§ããã
ãããŠR4ã¯äžè¬åŒ ïŒåŒäžãã¯å ã«å®çŸ©ããéãã§ããïŒã®åºãè¡š
ãããã®ååç©ãã補é ãããã åèšã®åå¿ã¯äžè¬åŒ X1COOR5 ïŒåŒäžãX1ã¯èçŽ ååãŸãã¯å¡©çŽ ååãè¡šããã
ããŠR5ã¯ïŒãïŒåã®ççŽ ååãæããã¢ã«ãã«
åºãè¡šããïŒã®ååç©ããã³é žå容äœäŸãã°ç¬¬ïŒ
ã¢ãã³äŸãã°ããªãšãã«ã¢ãã³ã®ååšäžã«å¥œãŸã
ãã¯â30âãªããïŒ30âã®æž©åºŠã§ããŸã奜ãŸãã
ã¯ç¡æ°ŽäžæŽ»æ§ææ©æº¶å€äŸãã°ã¯ãããã«ã ã®ååš
äžã«è¡ãããã ããã«æ¬çºæã®ç¹åŸŽã«ããã°ãR1ãããã³
R2ãå ã«å®çŸ©ããéãã§ããäžè¬åŒã®ååç©
ã¯ãã¢ã«ã³ãŒã«ãããšã¹ãã«ã補é ããããã®æ¢
ç¥ã®æ¹æ³ãé©çšãŸãã¯æ¡çšããããšã«ãã€ãŠãäŸ
ãã°äžè¬åŒ X2OCZCOYR3 ãåŒäžãX2ã¯èçŽ ååãŸãã¯å¡©çŽ ååãããã¯äž
è¬åŒ âOCOOR5 ïŒåŒäžãR5ã¯å ã«å®çŸ©ããéãã§ããïŒã®åºãè¡š
ããããããŠïŒºãããã³R3ã¯å ã«å®çŸ©ããé
ãã§ãããã®ååç©ãšåå¿ãããããšã«ãã€ãŠã
äžè¬åŒ ïŒR1ããã³ïŒ¡ã¯å ã«å®çŸ©ããéãã§ããïŒã®åå
ç©ãã補é ãããã åèšã®åå¿ã¯ãå Žåã«ããé žå容äœäŸãã°ç¬¬ïŒ
ã¢ãã³äŸãã°ããªãšãã«ã¢ãã³ãŸãã¯ç¡æ©å¡©åºäŸ
ãã°éçé žãããªãŠã ã®ååšäžã«ã奜ãŸããã¯ç¡
æ°ŽäžæŽ»æ§ææ©æº¶å€äŸãã°ã¯ãããã«ã ãŸãã¯ã¢ã»
ãã³ã®ååšäžã«ãããŠå¥œãŸããã¯â30âãªããïŒ
30âã®æž©åºŠã§è¡ãªãããã äžè¬åŒã®ååç©ã®éæ¯æ§é žä»å å¡©ã¯ææ©å¡©åº
ã®å¡©ã®è£œé ã®ããã«ç¥ãããæ¹æ³ãé©çšãŸãã¯æ¡
çšããããšã«ãã€ãŠãäŸãã°äžè¬åŒã®ååç©ã
é©çšãªæº¶å€äžã®é©åœãªé žãšåå¿ãããããšã«ãã€
ãŠè£œé ããŠããããå¡©ã¯æº¶æ¶²ã®èŠªæ¶²æ§åïŒãªãªã
ã€ãªãŒãŒã·ãšã³ïŒã«ãã€ãŠããããã¯åå¿åªäœäž
ã«åå溶解ãªããã°å¿ èŠã«ãã溶å€ã®äžéšãé€å»
ããåŸéã«ãã€ãŠåé¢ããŠãããã äžè¬åŒã®ååç©ã®æ··åç©ã¯ãæèäœçšæ··åç©
ã®åé¢ã®ããã®æ¢ç¥æ¹æ³ãé©çšãŸãã¯æ¡çšããã
ãšã«ãã€ãŠãäŸãã°ã¯ã¬ã€ã°ïŒCraigïŒè£ 眮ïŒãš
ãŒã»ãŠãšã€ã¹ãã«ã¬ãŒæ°èããã¯ããã¯ã»ãªãã»
ãªãŒã¬ããã¯ã»ã±ãã¹ããªã€ã第ïŒå·»ç¬¬286é ïŒ
ã§åæµååžæ³ã«ãã€ãŠãã¯ãããã°ã©ãã€ãŒïŒäŸ
ãã°ã¢ã«ãããŸãã¯ã·ãªã«ã²ã«äžã§ïŒã«ãã€ãŠã
ããã¯äŸãã°è³éŠæçåæ°ŽçŽ æº¶å€äŸãã°ãã³ãŒã³
ããã®åå¥çµæ¶ã«ãã€ãŠãäžè¬åŒã®æåååç©
ã«åé¢ããŠãããã äžè¬åŒã®ååç©ã¯ãžã«ã«ãã³é žãããšã¹ãã«
ã補é ããããã«ç¥ãããæ¹æ³ãé©çšãŸãã¯æ¡çš
ããããšã«ãã€ãŠãäŸãã°äžè¬åŒ ïŒåŒäžãã¯å ã«å®çŸ©ããéãã§ããïŒã®ååç©
ãšåå¿ãããããšã«ãã€ãŠãäžè¬åŒã®ååç©ã
ã補é ããŠãããã åèšã®åå¿ã¯äžæŽ»æ§ææ©æº¶å€ã®ååšäžã«å¥œãŸã
ãã¯å®€æž©ã«ãããŠå Žåã«ããå¡©åºäŸãã°ã¢ã«ã«ãª
éå±çé žå¡©ãŸãã¯éçé žå¡©ã第ïŒã¢ãã³ãããã¯
è€çŽ ç°åŒç¬¬ïŒå¡©åºäŸãã°ããªãžã³ã®ååšäžã§è¡ã€
ãŠãããã äžè¬åŒã®ååç©ã¯ãã¢ã«ã³ãŒã«ããã³ããªã³
ãŒã«ãããšã¹ãã«ã補é ããããã®æ¢ç¥æ¹æ³ãé©
çšãŸãã¯æ¡çšããããšã«ãã€ãŠãäŸãã°äžè¬åŒ
ã®ååç©ããäžè¬åŒã®ååç©ã補é ããããã«
å ã«è¿°ã¹ãæ¹æ³ãšåæ§ã«ããŠäžè¬åŒã®ååç©ã
äžè¬åŒïŒžã®ååç©ãšåå¿ãã次ãã§ã«ã«ãã³é žã
ãããã²ã³åã¢ã·ã«ãŸãã¯æ··åé žç¡æ°Žç©ã補é ã
ãããã®æ¢ç¥æ¹æ³ãé©çšãŸãã¯æ¡çšããããšã«ã
ã€ãŠäžè¿°ã®ããã«ããŠåŸããããããšã¹ãã«ãäž
è¬åŒã®ååç©ã«å€æããããšã«ãã€ãŠãäžè¬åŒ
ã®ååç©ãã補é ããŠãããã äžè¬åŒã«ãããŠR1ãæ°ŽçŽ ååãã¢ã»ãã«åº
ãŸãã¯ããããªãã«åºãè¡šããååç©ãããªãã¡
ããããã¹ãã©ãã€ã·ã³ãã¹ãã©ãã€ã·ã³ã
ãã³ã¹ãã©ãã€ã·ã³ãšããŠç¥ãããååç©ãã
ã³ãããã®æ··åç©ã¯ãã¹ãã©ãã€ã·ã³ã®è£œé ã®ã
ãã®æ¢ç¥æ¹æ³ãé©çšãŸãã¯æ¡çšããããšã«ãã€
ãŠãäŸãã°ã¹ãã¬ãããã€ã»ã¹ã»ã¢ã ããã¢ã·ã€
ãšã³ã¹ïŒStreptomyces ambofaciensïŒ
NRRL2420ãŸãã¯ãã®ã¹ãã©ãã€ã·ã³çç£æ§çªç¶
å€ç°æ§ã®å¥œæ°æ§å¹é€ã«ãã€ãŠè£œé ããŠãããã ã¹ãã¬ãããã€ã»ã¹ã»ã¢ã ããã¢ã·ã€ãšã³ã¹ã®
çºé µã«ãã€ãŠæ®éã«åŸãããã¹ãã©ãã€ã·ã³ã¯ã
ãããïŒçš®ã®ã¹ãã©ãã€ã·ã³ã®æ··åç©ã§ãããã
ãŸããããã®ã¹ãã©ãã€ã·ã³ã®ããããç¹ã«ã¹ã
ã©ãã€ã·ã³ãçºé µã«ãã€ãŠçŽæ¥åŸãããšãå¯èœ
ã§ãããProd.Pharm.17(4)ãïŒïŒ1962ïŒåç §ãã æ¬æ现æžã§çšãããããããªãæ¢ç¥æ¹æ³ããªã
èªã¯ãããèªäœãããŸã§ã«çšããããæ¹æ³ããã
ã¯ååŠæç®ã«èšèŒãããæ¹æ³ãæå³ããã äžè¬åŒã®ååç©ããã³ãããã®éæ¯æ§é žä»å
å¡©ã¯ãã¹ã¿ãã€ãã³ãã«ã¹ã»ã¢ãŠã¬ãŠã¹
ïŒStaphylococcus aureusïŒãèºççèããã³é£é
çèã®ææã«èµ·å ããææã®æ²»çãªãã³ã«ãã³ã
ã©ãºãã»ã¬ãªã»ããã€ã«ã ïŒMycoplasma
gallisepticumïŒãšããŠç¥ãããèžèèºçæ§çç©
ã«ãã€ãŠæ¬æ¥ããããããäžé¢é³¥ã®ãã³ãã©ã¹ã¢
ã·ã¹ïŒæææ§éèæŽçãŸãã¯æ°å¢çãšããŠãç¥ã
ããçŸç ïŒã®æ²»çã«ãããåç©è¬ãšããŠæ¥µããŠæ
çšãªããããæèæ§ãæããæçç©è³ªã§ããã äžè¬åŒã®ååç©ããã³ãããã®éæ¯æ§é žä»å
å¡©ã¯ãŸããåç©ããã³é³¥ç¹ã«å®¶èããã³å®¶çŠœäŸã
ã°è±ãçäŸãã°ä»çããã³å®¶çŠœäŸãã°é¶ããã³äž
é¢é³¥ã®çé·çããã³ïŒãŸãã¯ïŒé£Œæå€ææ¯ã®æ¹è¯
ã«æ¥µããŠæçšãªãã®ãšãããçé·ä¿é²æŽ»æ§ãæã
ããåç©ãŸãã¯é³¥ã®çé·çã«ããããã®ãããªæ¹
è¯ã¯ãéåžžå¿ èŠãšããããããçãæéã«äŸãã°
åžå Žã®ããã«æãŸããäœéã«éããããããã¯å
ãæéã«ããã€ãŠããã«äœéãå¢å ããããšãæ
å³ãããåç©ããã³é³¥ã®é£Œæå€ææ¯ã«ããããã®
ãããªæ¹è¯ã¯ãäžè¬åŒã®ååç©ãŸãã¯ãã®éæ¯
æ§é žä»å å¡©ãäžããããªãåæ§ãªåç©ãŸãã¯é³¥ã
ããç¹å®ãªäœéã«éããã®ã«é£Œæãå°ãªããŠãã
ããšãæå³ããããŸãã家犜ã«æäžãããå Žåã
äžè¬åŒã®ååç©ã¯åµã®çç£ãä¿é²ããã®ã«æçš
ã§ãããäžè¬åŒã®ååç©ã¯å¹³è¡¡é£ãšäžç·ã«åç©
ããã³é³¥ã«æäžãããã®ãæ®éã§ããã äžè¬åŒã®ååç©ã¯äžè¬åŒã®ååç©ãšåæ§ãª
çš®é¡ããã³çšåºŠã®æ²»çããã³çé·ä¿é²æŽ»æ§ãæ
ãããããŠããã«ãããã¯äžè¬åŒã®ååç©ãã
èŠå³ãå°ããããã¯å®éã«ã¯ç¡å³ã§ãããšããé
èŠãªå©ç¹ãæããããã®å©ç¹ã¯äžè¬åŒã®ååç©
ã®çµå£æäžã«ãã幌å ãåç©ããã³é³¥ãæ²»çãã
å Žåã«ç¹ã«éèŠã§ããã äžè¬åŒã®ååç©ã®éæ¯æ§é žä»å å¡©ã¯äžè¬åŒ
ã®ååç©ããèŠå³ãå°ããšããå©ç¹ããªãããã
ããªããããããã¯åœæ¥è ã«ãšã€ãŠããç¥ããã
æè¡ã«ãã€ãŠãäŸãã°æ°Žäžããã³ææ©æº¶å€äžã®å¡©
ãšããšã®å¡©åºãšã®éã®æº¶è§£åºŠã®å·®ãå©çšããŠãäž
è¬åŒã®ååç©ã粟補ããå Žåã«æçšã§ãããäž
è¬åŒã®ããšã®å¡©åºã¯æ¢ç¥æ¹æ³ã«ãã€ãŠäŸãã°é±
ç©å¡©åºäŸãã°æ°Žæ§æ°Žé žåãããªãŠã å¡©åºãšåŠçã
ãããšã«ãã€ãŠããããã®å¡©ããåçããããšã
ã§ããã è±åœç¹èš±ç¬¬796311å·æ现æžã«ã¯ãèèªæç³»ã®ãž
ã«ã«ãã³é žãšã¹ãã©ãã€ã·ã³ã«ãã€ãŠçæããã
ãããšã¹ãã«ãé瀺ãããŠãããããã®ãããšã¹
ãã«ã¯äžè¬åŒã®ååç©ããèŠå³ãå°ãã ããã§ããäžè¬åŒã®ååç©ã¯åèšã®è±åœç¹èš±
第796311å·æ现æžã«é瀺ãããååç©ãããäžå±€
èŠå³ãå°ãã äžè¬åŒã®ä»¥äžã®ååç©ããã³ãããã®æ··åç©
ã¯ç¹ã«èå³ãããã ååç© ã¹ãã©ãã€ã·ã³ã®ä»¥äžã®ååç© ïŒ¡ 2â²âãšãã«ãµã¯ã·ããŒã  2â²âãšãã«ã°ã«ã¿ã¬ãŒã  2â²âã¡ãã«ãµã¯ã·ããŒã  2â²âãããã«ãµã¯ã·ããŒã  2â²âã¡ãã«ã°ã«ã¿ã¬ãŒã  2â²âã¡ãã«ããã¬ãŒã  2â²ââã¡ãã«ããªãµã¯ã·ããŒã  2â²âã·ã¯ãããã·ã«ãµã¯ã·ããŒã  2â²âãšãã«ããããŒã  2â²âããšãã«ãµã¯ã·ããŒã  2â²âã¡ãã«ïŒRSïŒâïŒïŒâã¡ãã«ïŒãµã¯ã·ã
ãŒã ïŒ 2â²âïŒïŒâããšãã«ãšãã«ïŒãµã¯ã·ããŒã  2â²â第ïŒããã«ãµã¯ã·ããŒã  2â²âã€ãœãããã«ãµã¯ã·ããŒã  ã¹ãã©ãã€ã·ã³æ··åç©ã®2â²âãšãã«ãµã¯ã·ã
ãŒãããã㳠 ååç©ïŒ¡ã®äºå¡©é žå¡©  ååç©ïŒ¡ã®äºãããé žå¡© åèšïŒ¡ãããã³ïŒªãã®æåã¯æ¬æ现æžäžäŸ
ãã°ä»¥äžã®è¡šã«ãããåç §ã容æã«ããããã«å
åç©ã«éãããã äžè¬åŒã®ååç©ã«é¢ããŠè¡ãªãããè©Šéšç®¡å
ã®æ²»ç掻æ§ãšçç©æŽ»æ§ã«é¢ããå®éšã§ã¯ã以äžã®
è©ŠéšçµæãåŸãããã è©ŠéšïŒ çµå£çã«åŠçãããããŠã¹ ã ãã³ïŒ0.5mlïŒäžã«æžæ¿ãããã¹ã¿ãã€ãã³
ãã«ã¹ã»ã¢ãŠã¬ãŠã¹ã¹ãã¹ïŒStaphylococcus
aureus SmithïŒãããŠã¹ã®è ¹è å ã«æ³šå°ããã
ãã®ææã¯100ïŒ ã®ããŠã¹ãæ»äº¡ãããããã«èš
ç®ãããæå°æäžéã®çŽ100åã瀺ãããããŠã¹
ã«äžè¬åŒã®ååç©ãåç©ã®äœéïŒKgåœãããã
ã16mgã31mgã62.5mgã125mgãŸãã¯250mgçµå£æ
äžãããææåŸïŒãïŒã24ã30ããã³48æéã«ã
æäžéãéžæããŠããããã®ããŠã¹ã«æäžããã
ææåŸæ°æ¥éããŠã¹ã芳å¯ããã äžè¬åŒã®ååç©ããåŸãããçµæã¯æšæºåå
ç©ãšããŠçšããããã¹ãã©ãã€ã·ã³ããåŸãã
ãçµæãšæ¯èŒãããã åŸãããçµæã¯ä»¥äžã®è¡š(ii)ã«ç€ºãããã ãCD50ããªãèªã¯ãåŠçãããããŠã¹ã®50ïŒ
ãæ²»çããããã«èšç®ãããæäžéãæå³ããã è©ŠéšïŒ ç®äžçã«åŠçãããããŠã¹ ã ãã³ïŒ0.5mlïŒäžã«æžæ¿ãããã¹ã¿ãã€ãã³
ãã«ã¹ã»ã¢ãŠã¬ãŠã¹ã¹ãã¹ãææãããããŠã¹
100ïŒ ãæ»ã«èŽãããæå°éã®çŽ100åãã§ããŠã¹
ã®è ¹è å ãææããããææåŸïŒãïŒã24ã30ã
ãã³48æéã«äžè¬åŒã®ååç©ãåç©ã®äœéïŒKg
åœã1.6mgã3.1mgã6.25mgã12.5mgãŸãã¯25mgã§
ããŠã¹ãããããç®äžçã«åŠçãããææåŸæ°æ¥
éããŠã¹ã芳å¯ããã äžè¬åŒã®ååç©ããåŸãããçµæãæšæºåå
ç©ãšããŠçšããããã¹ãã©ãã€ã·ã³ããåŸãã
ãçµæãšæ¯èŒãããããªãã¡ãååç©ïŒ¡ã¯åç©ã®
äœéïŒKgåœã17.5mgã®CD50ã瀺ããããã¹ãã©
ãã€ã·ã³ã¯åç©ã®äœéïŒKgåœã5.8mgã®CD50ã
瀺ããã è©ŠéšïŒ è©Šéšç®¡å æŽ»æ§ åè©Šéšååç©ã«å¯ŸããŠãããªããã³å€§è±æ±äžã®
ããç¯å²ã®æ¿åºŠã調補ãããç°ã€ãæ¿åºŠã®è©Šéšå
åç©ããã®ç¯å²å ã§å«æããããããã®è©Šéšç®¡ã«
ã¹ã¿ãã€ãã³ãã«ã¹ã»ã¢ãŠã¬ãŠã¹ïŒãªãã¯ã¹ããª
ãŒãïŒãŸãã¯ãšã³ãšãªãã¢ã»ã³ãªïŒ¢ïŒãšã³ãã¹ïŒ
ãEscherichia coli ïŒEnnisïŒãïŒè©Šéšç®¡ïŒæ¬åœ
ãçŽ105åã®åŸ®çç©ïŒããéžã°ããè©Šéšçç©ãæ¥
çš®ããããŠ37âã§ã€ã³ããŠããŒã·ãšã³ããåŸè©Šéš
çç©ã®çé·ãæå¶ããã®ã«å¿ èŠãšãããç¯å²å ã®
è©Šéšååç©ã®æäœæ¿åºŠããã®ååç©ãšãã®çç©ã«
察ããæå°æå¶æ¿åºŠãšããŠèšé²ããã åŸãããçµæã以äžã®è¡š(i)ã«èšèŒããã
ãè¡šã
ã³
ãããã®çµæã¯äžè¬åŒã®ååç©ãäžè¬åŒã®
ååç©ãšåçšåºŠã®æŽ»æ§ãæããããšã瀺ããŠã
ãã 以äžã®è©Šéšã«ãã€ãŠç€ºãããããã«ãäžè¬åŒ
ã®ååç©ã¯åºä¹³åç©ã«å¯ŸããŠæ¯èŒçç¡å®³ã§ãããš
ããäºå®ã«ãã€ãŠãããã®æçç©è³ªããã³çé·ä¿
é²å€ãšããŠã®æçšæ§ãé«ããããã ããŠã¹æ¯æ§ äžè¬åŒã®ååç©ã®ïŒã€ã«ãã€ãŠããŠã¹ããã
ããçµå£çã«åŠçããããŠæ¬¡ã®ïŒæ¥éãããã芳
å¯ããã åŸãããLD50å€ïŒåŠçãããããŠã¹ã®50ïŒ ã
æ»äº¡ããããéïŒãåç©ã®äœéïŒKgåœãã®mgãšã
ãŠè¡šã«ç€ºããåç©ã®äœéïŒKgåœã1000mgãæäž
ããŠãå®éšã®éããŠã¹ã¯å šç¶æ»äº¡ããªãã€ãã è¡š ååç© LD50  ïŒ1000  ïŒ1000 èšå·ãïŒãã¯æ¬æ现æžã§ã¯ããã倧ããããæ
å³ããã 以äžã®äŸã¯æ¬çºæã«ããæ°èŠååç©ã®è£œé ã説
æãããã®ã§ããã äŸ ïŒ ååç©ïŒ¡ 也ç¥ã¯ãããã«ã ïŒ50mlïŒäžã®ãšãã«ïŒâã¯ã
ããã«ãã«ããããªããŒãïŒ3.2ïœïŒäžã®æº¶æ¶²
ãã也ç¥ã¯ãããã«ã ïŒ100mlïŒäžã®ã¹ãã©ãã€
ã·ã³ïŒ15ïœïŒã®æº¶æ¶²ã«ãããŸããªãã滎å ã
ãã枩床ãæ·»å ã®é氷济äžã§å·åŽããªããïŒâã
10âã®æž©åºŠã«ç¶æãããæ··åç©ã宀枩ã«ïŒæéæŸ
眮ããã溶å€ãç空äžã§é€å»ããããŠæ®çç©ãæ°Ž
æ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒãšäžç·ã«æ¯çªãããåŸ
ãããæ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœ
åºãããæ°Žæ§å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããª
ãŠã 溶液ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽããã次
ã«ãæ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœåº
ããããŠãã®ãšãŒãã«æœåºç©ãç¡«é žãã°ãã·ãŠã
äžã§ä¹Ÿç¥ããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»
ããŠã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ãµã¯ã·ããŒã
ïŒ4.5ïœïŒãçœè²åºäœïŒèç¹100ãã110âïŒã®åœ¢ã§
åŸãã äŸ ïŒ ååç©ïŒ¢ ããªãšãã«ã¢ãã³ïŒïŒïœïŒããã³ä¹Ÿç¥ã¯ããã
ã«ã ïŒ50mlïŒäžã®ãšãã«ïŒâã¯ãããã«ãã«ãã
ã¬ãŒãïŒ5.1ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ãããã«ã
ïŒ100mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ24ïœïŒã®æº¶æ¶²ã«
ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ããã
枩床ã¯æ·»å ã®é氷济äžã§å·åŽããªããïŒâã10â
ã®æž©åºŠã«ç¶æãããæ··åç©ã宀枩ã«ïŒæéæŸçœ®ã
ãã溶å€ãç空äžã§é€å»ããããŠæ®çç©ãæ°Žæ§é ¢
é žæº¶æ¶²ïŒ100mlã1NïŒãšäžç·ã«æ¯çªãããåŸãã
ãæ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœåºã
ããæ°Žæ§å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã
溶液ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽããã次ã«ã
æ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœåºãã
ããŠãã®ãšãŒãã«æœåºç©ãç¡«é žãã°ãã·ãŠã äžã§
也ç¥ãããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»ã
ãŠã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã
ïŒ3.0ïœïŒãçœè²åºäœïŒèç¹87ãã95âïŒã®åœ¢ã§åŸ
ãã äŸ ïŒ ååç©ïŒ£ ããªãšãã«ã¢ãã³ïŒ1.0ïœïŒããã³ä¹Ÿç¥ã¯ãã
ãã«ã ïŒ10mlïŒäžã®ã¡ãã«ïŒâã¯ãããã«ãã«ã
ãããªããŒãïŒ1.7ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ããã
ã«ã ïŒ100mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®
溶液ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å
ãããæ··åç©ãïŒæéãããŸã次ã«æº¶å€ãç空äž
ã§é€å»ãããåŸãããæ®çç©ã«æ°Žæ§é ¢é žæº¶æ¶²ïŒ75
mlã1NïŒãšãžãšãã«ãšãŒãã«ïŒ100mlïŒãå ã
ããåºäœã溶解ãããŸã§æ··åç©ãæ¯çªãããæ°Žæ§
å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液
ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽããã次ã«æ··åç©
ããžãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºããããŠãš
ãŒãã«æœåºç©ãç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ããã
ãžãšãã«ãšãŒãã«ãæžå§äžã§çå»ããŠã¹ãã©ãã€
ã·ã³ã®2â²âã¡ãã«ãµã€ã¯ã·ããŒãïŒ1.0ïœïŒã
çœè²åºäœïŒèç¹108ãã118âïŒã®åœ¢ã§åŸãã äŸ ïŒ ååç©ïŒ€ 也ç¥ã¯ãããã«ã ïŒ10mlïŒäžã®ãããã«ïŒâã¯
ãããã«ãã«ããããªããŒãïŒ1.8ïœïŒã®æº¶æ¶²ã
也ç¥ã¯ãããã«ã ïŒ70mlïŒäžã®ã¹ãã©ãã€ã·ã³
ïŒ8.4ïœïŒã®æº¶æ¶²ã«ãããŸããªãã滎å ãããæ··å
ç©ãããã«ïŒæéãããŸã次ã«æº¶å€ãç空äžã§é€
å»ãããæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒäž
ã«æº¶è§£ããããŠåŸãããæ··åç©ããžãšãã«ãšãŒã
ã«ïŒ100mlïŒã§æœåºãããæ°Žæ§å±€ãåé¢ããããŠ
æ°Žæ§æ°Žé žåãããªãŠã 溶液ïŒ10NïŒãå ããŠçŽPH
ïŒã«èª¿æŽãããæ··åç©ããžãšãã«ãšãŒãã«ïŒ100
mlïŒã§æœåºããããŠãã®ãšãŒãã«æœåºç©ãç¡«é žã
ã°ãã·ãŠã äžã§ä¹Ÿç¥ãããããžãšãã«ãšãŒãã«ã
æžå§äžã§çå»ããŠã¹ãã©ãã€ã·ã³ã®2â²âããã
ã«ãµã¯ã·ããŒãïŒ0.7ïœïŒãçœè²åºäœïŒèç¹88ã
ã96âïŒã®åœ¢ã§åŸãã äŸ ïŒ ååç©ïŒ¥ ããªãšãã«ã¢ãã³ïŒ1.0ïœïŒããã³ä¹Ÿç¥ã¯ãã
ãã«ã ïŒ20mlïŒäžã®ã¡ãã«ïŒâã¯ãããã«ãã«ã
ãã¬ãŒãïŒ1.65ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ãããã«ã
ïŒ70mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶æ¶²ã«
ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ããã
æ··åç©ãããã«ïŒæéãããŸããããŠ17æéæŸçœ®
ãããåå¿æ··åç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒ
ã§æœåºãããã¯ãããã«ã å±€ãåé¢ããããŠïŒ%
ïœïŒïœæ°Žæ§çé žãããªãŠã 溶液ïŒ100mlïŒã§æŽæµã
ãã次ã«ãã¯ãããã«ã ãç¡«é žãã°ãã·ãŠã äžã§
也ç¥ããããã¯ãããã«ã ãæžå§äžã§çå»ããŠã¹
ãã©ãã€ã·ã³ã®2â²âã¡ãã«ã°ã«ã¿ã¬ãŒãïŒ3.2
ïœïŒãé»è€è²åºäœïŒèç¹85ãã95âïŒã®åœ¢ã§åŸ
ãã äŸ ïŒ ååç©ïŒŠ éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ40mlïŒäžã®ã¡ãã«ãã©ã³ã¹âïŒâã¯ãããã«ã
ã«ã¢ã¯ãªã¬ãŒãïŒ2.2ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»ã
ã³ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶
液ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ã
ããæ··åç©ãããã«ïŒæéãããŸãã17æéæŸçœ®
ã次ã«åºäœãå»ããã液ãèžçºãããããŠæ®
çç©ãé ¢é žãšãã«ïŒ100mlïŒäžã«æº¶è§£ããããã
ã®æº¶æ¶²ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã1NïŒã§æœåºã
ããæ°Žæ§å±€ãåé¢ããããŠã¯ãããã«ã ïŒïŒÃ50
mlïŒã§æœåºãããäžç·ã«ããã¯ãããã«ã æœåºç©
ãæ°ŽïŒ100mlïŒïŒ%w/væ°Žæ§éçé žãããªãŠã 溶
液ïŒ100mlïŒã§æŽæµããã次ã«ãã¯ãããã«ã å±€
ãç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ããããã¯ãããã«
ã ãæžå§äžã§çå»ããŠã¹ãã©ãã€ã·ã³ã®2â²âã¡
ãã«ããã¬ãŒãïŒ2.7ïœïŒïŒèç¹105ãã115âïŒã
åŸãã äŸ ïŒ ååç©ïŒ§ ããªãšãã«ã¢ãã³ïŒ1.1ïœïŒããã³ãšãã«ã¯ã
ããã«ã¡ãŒãïŒ1.1ïœïŒããã³ä¹Ÿç¥ã¯ãããã«ã
ïŒ50mlïŒäžã®ã¡ã¿ã³ããªãŒã«ïŒ1.0ïœïŒã®æº¶æ¶²ãã
â15âã«å·åŽããã也ç¥ã¯ãããã«ã ïŒ80mlïŒäž
ã®ã¹ãã©ãã€ã·ã³2â²âãããµã¯ã·ããŒãïŒ9.4
ïœïŒã®æº¶æ¶²ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Ž
ã¥ã€å ãããæ·»å ã®éã枩床ãâ15âãªããâ10
âã®æž©åºŠã«ç¶æãããæ··åç©ã宀枩ãŸã§å æž©ãã
ããŠ18æéæŸçœ®ããã溶å€ãæžå§äžã§èžçã«ãã€
ãŠé€å»ããããžãšãã«ãšãŒãã«ïŒ100mlïŒãæ®ç
ç©ã«å ããããŠäžæº¶æ§ç©è³ªãå»ãããéç©ã
æ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒã§æœåºãããæ°Žæ§å±€
ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液
ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽãããæ··åç©ããž
ãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºããããŠãžãšã
ã«ãšãŒãã«æœåºç©ãïŒ%w/væ°Žæ§éçé žãããªãŠ
ã 溶液ïŒ100mlïŒã§æŽæµããç¡«é žãã°ãã·ãŠã äž
ã§ä¹Ÿç¥ãããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»
ããŠã¹ãã©ãã€ã·ã³ã®2â²ââã¡ãã«ããªãµã¯
ã·ããŒãïŒ1.4ïœïŒïŒèç¹114ãã124âïŒãåŸãã åèšã®è£œé äŸã§çšããããã¹ãã©ãã€ã·ã³ã®
2â²âãã¯ãé žãããšã¹ãã«ã次ã®ããã«ããŠè£œé
ããã ç¡æ°Žãã¯ãé žïŒ6.0ïœïŒãé ¢é žãšãã«ïŒ600mlïŒ
äžã®ã¹ãã©ãã€ã·ã³ïŒ50.4ïœïŒã®æº¶æ¶²ã«ãããŸ
ããªããå ãããæ··åç©ãããã«ïŒæéãããŸã
ãããŠå®€æž©ã«18æéæŸçœ®ããã溶å€ãç空äžã§é€
å»ããããŠãžãšãã«ãšãŒãã«ïŒ200mlïŒãæ®çç©
ã«å ãããåºäœãå»ã也ç¥ãããŠã¹ãã©ãã€ã·
ã³ã®2â²âãã¯ãé žãããšã¹ãã«ïŒ38.6ïœïŒïŒè
ç¹140ãã148âïŒãåŸãã äŸ ïŒ ååç©ïŒš éçé žãããªãŠã ïŒ4.0ïœïŒããã³ä¹Ÿç¥ã¢ã»ã
ã³ïŒ10mlïŒäžã®ã·ã¯ãããã·ã«ïŒâã¯ãããã«ã
ã«ããããªããŒãïŒ2.4ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»
ãã³ïŒ70mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®
溶液ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å
ãããæ··åç©ãïŒæéãããŸã次ã«16æéæŸçœ®ã
ããæ··åç©ãéããããŠæ¶²ãæžå§äžã§èžçºã
ãããæ®çç©ãé ¢é žãšãã«ïŒ70mlïŒäžã«æº¶è§£ãã
ããŠåŸãããæ··åç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã
0.5NïŒã§æœåºãããæ°Žæ§å±€ãåé¢ããããŠã¯ã
ããã«ã ïŒïŒÃ50mlïŒã§æœåºãããäžç·ã«ããã¯
ãããã«ã æœåºç©ãæ°ŽïŒ100mlïŒãšïŒ%w/væ°Žæ§
éçé žãããªãŠã 溶液ïŒ75mlïŒã§æŽæµãããããŠ
ç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ããããã¯ãããã«ã
ãç空äžã§èžçºé€å»ããŠã¹ãã©ãã€ã·ã³ã®2â²â
ã·ã¯ãããã·ã«ãµã¯ã·ããŒãïŒ3.4ïœïŒïŒèç¹90ã
ã100âïŒãåŸãã äŸ ïŒ ååç©ïŒª éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ20mlïŒäžã®ãšãã«ïŒâã¯ãããã«ãã«ã¢ã»ããŒ
ãïŒ1.5ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»ãã³ïŒ75mlïŒäž
ã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶æ¶²ã«ãããŸã
ãªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ãããæ¹æãïŒ
æéç¶ã次ã«æ··åç©ã17æéæŸçœ®ãããåºäœã
å»ããããŠæ¶²ãç空äžã§èžçºããããæ®çç©ã
é ¢é žãšãã«ïŒ100mlïŒäžã«æº¶è§£ããããŠãã®æº¶æ¶²
ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã0.5NïŒã§æœåºãããæ°Ž
æ§å±€ãåé¢ããããŠã¯ãããã«ã ïŒïŒÃ50mlïŒã§
æœåºãããã¯ãããã«ã æœåºç©ãäžç·ã«ããæ°Ž
ïŒ100mlïŒããã³ïŒ%w/væ°Žæ§éçé žãããªãŠã 溶
液ïŒ100mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äž
ã§ä¹Ÿç¥ããããã¯ãããã«ã ãæžå§äžã§çå»ããŠ
ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ããããŒãïŒ2.0
ïœïŒïŒèç¹89ãã99âïŒãåŸãã äŸ 10 ååç©ïŒ« ããªãšãã«ã¢ãã³ïŒ1.5ïœïŒããã³ãšãã«ã¯ã
ããã«ã¡ãŒãïŒ1.7ïœïŒããâ15âã«å·åŽããã也
ç¥ã¯ãããã«ã ïŒ50mlïŒäžã®ããšãã«æ°ŽçŽ ãµã¯ã·
ããŒãïŒ2.7ïœïŒã®æº¶æ¶²ã«ãããŸããªããå ã
ãã也ç¥ã¯ãããã«ã ïŒ30mlïŒäžã®ã¹ãã©ãã€ã·
ã³ïŒ8.4ïœïŒã®æº¶æ¶²ãåŸãããæ··åç©äžã«æ»Žå
ãããããšåæã«æ··åç©ã®æž©åºŠãâ15âãªããâ
10âã«ç¶æãããæ··åç©ã宀枩ãŸã§å æž©ããããŠ
17æéæŸçœ®ãããæ··åç©ãç空äžã§èžçºãããã
ããŠé ¢é žãšãã«ïŒ75mlïŒãšïŒ%w/væ°Žæ§éçé žã
ããªãŠã 溶液ïŒ75mlïŒãæ®çç©ã«å ãããææ©å±€
ãåé¢ããããŠæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã0.5NïŒã§
æœåºããã次ã«ãæ°Žæ§å±€ãã¯ãããã«ã ïŒïŒÃ50
mlïŒã§æœåºãããäžç·ã«ããã¯ãããã«ã æœåºç©
ãæ°ŽïŒ100mlïŒãšïŒ%w/væ°Žæ§éçé žãããªãŠã
溶液ïŒ75mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äž
ã§ä¹Ÿç¥ããããæžå§äžã§ã¯ãããã«ã ãèžçºãã
ãŠã¹ãã©ãã€ã·ã³ã®2â²âããšãã«ãµã¯ã·ããŒã
ïŒ5.1ïœïŒïŒèç¹105ãã115âïŒãåŸãã äŸ 11 ååç©ïŒ¬ éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ20mlïŒäžã®ã¡ãã«ïŒRSïŒâïŒâã¯ãããã«ãã«
âïŒâã¡ãã«ããããªããŒãïŒ2.5ïœïŒã®æº¶æ¶²
ãã也ç¥ã¢ã»ãã³ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³
ïŒ8.4ïœïŒã®æº¶æ¶²ã«ãããŸããªããå ãããåŸè ã¯
ïŒæ»Žã¥ã€å ããããããŸããïŒæéç¶ã次ã«æ··å
ç©ã18æéæŸçœ®ãããåºäœãå»ããããŠæ¶²ã
ç空äžã§èžçºããããæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75
mlã0.5NïŒäžã«æº¶è§£ãããåŸãããæ··åç©ãé ¢
é žãšãã«ïŒïŒÃ50mlïŒãããŠæ¬¡ã«ã¯ãããã«ã
ïŒïŒÃ50mlïŒã§æœåºãããäžç·ã«ããã¯ãããã«
ã æœåºç©ãæ°ŽïŒ100mlïŒããã³ïŒ%w/væ°Žæ§éç
é žãããªãŠã 溶液ïŒ100mlïŒã§æŽæµããããŠç¡«é ž
ãã°ãã·ãŠã äžã§ä¹Ÿç¥ããããã¯ãããã«ã ãæž
å§äžã§çå»ããŠã¹ãã©ãã€ã·ã³ã®2â²âã¡ãã«
ïŒRSïŒâïŒïŒâã¡ãã«ïŒãµã¯ã·ããŒãïŒ1.7ïœïŒïŒè
ç¹92ãã102âïŒãåŸãã äŸ 12 ååç©ïŒ ããªãšãã«ã¢ãã³ïŒ1.1ïœïŒããã³ãšãã«ã¯ã
ããã«ã ã¡ãŒãïŒ1.1ïœïŒããâ15âã«å·åŽããã
也ç¥ã¯ãããã«ã ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³
2â²âãããµã¯ã·ããŒãïŒ9.4ïœïŒã®æº¶æ¶²ã«ãããŸ
ããªããå ããã枩床ãâ15âãªããâ10âã«ç¶
æããªããã也ç¥ã¯ãããã«ã ïŒ20mlïŒäžã®ïŒâ
ããšãã«ãšã¿ããŒã«ïŒ1.8ïœïŒã®æº¶æ¶²ã滎å ã
ãã次ã«ãæ··åç©ã宀枩ãŸã§å æž©ããããŠ18æé
æŸçœ®ããã溶å€ãæžå§äžã§é€å»ããããŠæ®çç©ã
æ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒäžã«æº¶è§£ããã溶液
ããžãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºãããæ°Žæ§
å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液
ïŒ10NïŒã§çŽPHïŒã«èª¿æŽãããæ··åç©ããžãšãã«
ãšãŒãã«ïŒ100mlïŒã§æœåºããããŠãã®ãšãŒãã«
æœåºç©ãïŒ%w/væ°Žæ§éçé žãããªãŠã 溶液
ïŒ100mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿ
ç¥ãããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»ããŠ
ã¹ãã©ãã€ã·ã³ã®2â²âïŒïŒâããšãã«ãšãã«ïŒ
ãµã¯ã·ããŒãïŒ2.3ïœïŒïŒç¹58ã68âïŒãåŸãã äŸ 13 ååç©ïŒ® ããªãšãã«ã¢ãã³ïŒ1.1ïœïŒããã³ãšãã«ã¯ã
ããã«ã¡ãŒãïŒ1.1ïœïŒãâ15âã«å·åŽããã也
ç¥ã¯ãããã«ã ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³
2â²âãããµã¯ã·ããŒãïŒ9.4ïœïŒã®æº¶æ¶²ã«ãããŸ
ããªããå ããã次ã«ã枩床ãâ15âãªããâ10
âã«ç¶æããªããã也ç¥ã¯ãããã«ã ïŒ40mlïŒäž
ã®ç¬¬ïŒãã¿ããŒã«ïŒ1.2mlïŒã®æº¶æ¶²ãåŸãããæ··
åç©ã«æ»Žå ãããåå¿ç©ã宀枩ãŸã§æž©ããªããã
ãããŸããç¶ã次ã«æ··åç©ã18æéæŸçœ®ããã溶
å€ãæžå§äžã§é€å»ããããŠæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²
ïŒ100mlã1NïŒäžã«æº¶è§£ãããæ··åç©ããžãšãã«
ãšãŒãã«ïŒ100mlïŒã§æœåºãããæ°Žæ§å±€ãåé¢ã
ãããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液ïŒ10NïŒã§çŽPH
ïŒã«èª¿æŽãããæ··åç©ããžãšãã«ãšãŒãã«ïŒ100
mlïŒã§æœåºããããŠãã®ãšãŒãã«æœåºç©ãïŒ%
ïœïŒïœæ°Žæ§éçé žãããªãŠã 溶液ïŒ100mlïŒã§æŽæµ
ããããŠç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ãããããžãš
ãã«ãšãŒãã«ãæžå§äžã§çå»ããŠã¹ãã©ãã€ã·ã³
ã®2â²â第ïŒããã«ãµã¯ã·ããŒãïŒ0.9ïœïŒïŒèç¹
99ãã108âïŒãåŸãã äŸ 14 ååç©ïŒ¯ 也ç¥ã¯ãããã«ã ïŒ10mlïŒäžã®ããªãšãã«ã¢ã
ã³ïŒ1.0ïœïŒã®æº¶æ¶²ããã³ä¹Ÿç¥ã¯ãããã«ã ïŒ200
mlïŒäžã®ã€ãœãããã«ïŒâã¯ãããã«ãã«ããã
ãªããŒãïŒ2.2ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ãããã«ã
ïŒ75mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶æ¶²ã«
ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ããã
æ··åç©ãïŒæéãããŸã次ã«18æéæŸçœ®ããã溶
å€ãæžå§äžã§é€å»ãããããŠæ°Žæ§é ¢é žæº¶æ¶²ïŒ100
mlã1NïŒããã³ãžãšãã«ãšãŒãã«ïŒ100mlïŒãæ¯
çªãããŠå ãããæ°Žæ§å±€ãåé¢ããããŠæ°Žæ§æ°Žé ž
åãããªãŠã 溶液ïŒ10NïŒã§çŽPHïŒã«èª¿æŽããã
æ··åç©ããžãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºãã
ããŠãã®ãšãŒãã«æœåºç©ãïŒ%w/væ°Žæ§éçé žã
ããªãŠã 溶液ïŒ100mlïŒã§æŽæµããããŠç¡«é žãã°
ãã·ãŠã äžã§ä¹Ÿç¥ãããããžãšãã«ãšãŒãã«ãæž
å§äžã§é€å»ããŠã¹ãã©ãã€ã·ã³ã®2â²âã€ãœãã
ãã«ãµã¯ã·ããŒãïŒ1.2ïœïŒïŒèç¹105ãã115âïŒ
ãåŸãã äŸ 15 ååç©ïŒ° éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ30mlïŒäžã®ãšãã«ïŒâã¯ãããã«ãã«ããããª
ããŒãïŒ2.5ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»ãã³ïŒ80
mlïŒäžã®ã¹ãã©ãã€ã·ã³æ··åç©ïŒ8.4ïœïŒã®æº¶æ¶²
ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ã
ããæ··åç©ãïŒæéãããŸã次ã«18æéæŸçœ®ã
ããåºäœãå»ããããŠæ¶²ãæžå§äžã§èžçºãã
ããæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã0.5NïŒäžã«
溶解ããããŠåŸãããæ··åç©ãé ¢é žãšãã«ïŒïŒÃ
50mlïŒæ¬¡ã«ã¯ãããã«ã ïŒïŒÃ50mlïŒã§æœåºã
ããäžç·ã«ããã¯ãããã«ã æœåºç©ãæ°ŽïŒ100
mlïŒãšïŒ%w/væ°Žæ§éçé žãããªãŠã 溶液ïŒ100
mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ã
ãããã¯ãããã«ã ãæžå§äžã§é€å»ããŠã¹ãã©ã
ã€ã·ã³æ··åç©ã®2â²âãšãã«ãµã¯ã·ããŒãïŒ4.4
ïœïŒïŒèç¹80ãã90âïŒãåŸãã åèšã®è£œé äŸã§çšããããã¹ãã©ãã€ã·ã³æ··å
ç©ã¯ãçŽ60%w/vã¹ãã©ãã€ã·ã³ã20%w/wã¹
ãã©ãã€ã·ã³ããã³20%w/wã¹ãã©ãã€ã·ã³
ãããªããã®ã§ãã€ãã äŸ 16 ååç©ïŒ± ãšã¿ããŒã«äžã®å¡©åæ°ŽçŽ ã®æº¶æ¶²ãããã¯ãåºäœ
ãæ²æ®¿ããªããªããŸã§ãžãšãã«ãšãŒãã«ïŒ50mlïŒ
äžã®ã¹ãã©ãã€ã·ã³ïŒ1.5ïœïŒã®2â²âãšãã«ãµ
ã¯ã·ããŒãã®æº¶æ¶²ã«æ»Žå ãããçœè²åºäœãå»
ãããžãšãã«ãšãŒãã«ã§æŽæµããããŠä¹Ÿç¥ãããŠ
ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ãµã¯ã·ããŒãã®äº
å¡©é žå¡©ïŒ1.4ïœïŒïŒèç¹64ãã74âïŒãåŸãã äŸ 17 ååç©ïŒ² ãžãšãã«ãšãŒãã«äžã®ãããé žã®é£œå溶液ãã
ãã¯ãåºäœãæ²æ®¿ããªããªããŸã§ããžãšãã«ãšãŒ
ãã«ïŒ50mlïŒäžã®ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«
ãµã¯ã·ããŒãïŒ1.5ïœïŒã®æº¶æ¶²ã«æ»Žå ãããçœè²
åºäœãå»ãããžãšãã«ãšãŒãã«ã§æŽæµããããŠ
也ç¥ãããŠã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ãµã¯ã·
ããŒãã®äºãããé žå¡©ïŒ1.6ïœïŒïŒèç¹85ãã97
âïŒãåŸãã æ¬çºæã¯çžå®¹æ§ã®è£œè¬çã«èš±å®¹ãåŸãæ äœãŸã
ã¯å€ç®ãšå ±ã«äžè¬åŒã®å°ããšãïŒçš®ã®ååç©ã
å«æãã補è¬æçç©çµæç©ããã®ç¯å²å ã«å å«ã
ããäžè¬åŒã®ååç©ã¯ãçŽè žå ã«ãè å ã«ãé
çµå£çã«ããã«ç¹ã«ã¯çµå£çã«æäžãããã®ãæ®
éã§ããã çµå£æäžçšã®åºäœçµæç©ã¯å§çž®é å€ããã«ãå
æ£æ§ç²æ«ããã³é¡ç²ãå å«ããããã®ãããªåºäœ
çµæç©äžã§ãïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§ååç©
ã¯ããã³ããããçé žã«ã«ã·ãŠã ããããããã§
ãã·ããã¢ã«ã®ã³é žãŸãã¯ä¹³ç³ã®ãããªå°ããšã
ïŒçš®ã®äžæŽ»æ§åžéå€ãšæ··åãããããŸããéåžžå®
æœãããããã«çµæç©ã¯äžæŽ»æ§åžéå€ä»¥å€ã®è¿œå
ã®ç©è³ªäŸãã°ã¹ãã¢ãªã³é žãã°ãã·ãŠã ã®ãããª
æ»å€ãããªã€ãŠããŠããããçµå£æäžçšã®æ¶²äœçµ
æç©ã¯ã補è¬çã«èš±å®¹ãåŸãä¹³å€ã溶液ãæžæ¿
液ãã·ãããããã³åœæè¡åéã§æ®éã«çšããã
ãäžæŽ»æ§åžéå€äŸãã°æ°Žããã³æ¶²äœãã©ãã€ã³ã
å«æãããšãªãã·ã«ãå å«ãããäžæŽ»æ§åžéå€ä»¥
å€ã«ããŸããã®ãããªçµæç©ã¯è£å©å€äŸãã°æ¹¿æœ€
å€ãæžæ¿å€ãçå³å€ãéŠå³å€ãéŠæããã³ä¿åå€
ãããªã€ãŠããŠãããããŸããæ¬çºæã«ããçµå£
æäžçšã®çµæç©ã¯ãåžéå€ãŸãã¯è³Šåœ¢å€ãæ·»å ãŸ
ãã¯æ·»å ããŠããªãïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§ç©
質ãå«æãããŒã©ãã³ã®ãããªåžåæ§ç©è³ªã®ã«ã
ã»ã«ãå å«ããã çµå£æäžçšã®çµæç©ã¯ãããèªäœæ¢ç¥ã®æ¹æ³
ïŒãã®ãããªæ¹æ³ã¯ãããŸã§çšããããæ¹æ³ãŸã
ã¯æç®ã«èšèŒãããæ¹æ³ãšå®çŸ©ãããïŒã§èª¿åã
ããé¡ç²ïŒããã¯äœ¿çšåã«æ°Žäžã«åæ£ãããŠããŠ
ãããïŒãå å«ããã è å æäžçšã®åºäœçµæç©ã¯ããèªäœæ¢ç¥ã®æ¹æ³
ã§èª¿åãããããŠïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§åå
ç©ãå«æãããããµãªãŒãå å«ããã çŽè žå æäžçšã®åºäœçµæç©ã¯ããèªäœæ¢ç¥ã®æ¹
æ³ã§èª¿åãããããŠïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§å
åç©ãå«æããåè¬ãå å«ããã éçµå£æäžçšã®æ¬çºæã«ãã補å€ã¯æ» èæ°Žæ§ãŸ
ãã¯éæ°Žæ§æº¶æ¶²ãæžæ¿æ¶²ãŸãã¯ä¹³å€ãå å«ããã
éæ°Žæ§æº¶å€ãŸãã¯æžæ¿åªäœã®äŸã¯ããããã¬ã³ã°
ãªã³ãŒã«ãããªãšãã¬ã³ã°ãªã³ãŒã«ããªãªãŒãæ²¹
ã®ãããªæ€ç©æ²¹ããã³ãªã¬ã€ã³é žãšãã«ã®ãããª
泚å°å¯èœãªææ©ãšã¹ãã«ã§ããããŸãããããã®
çµæç©ã¯è£å©å€äŸãã°ä¿åå€ã湿最å€ãä¹³åå€ã
ãã³åæ£å€ãå å«ããŠããŠãããããããã¯äŸã
ã°ãã¯ããªã¢ä¿æãã€ã«ã¿ãŒãéããŠã®éã«ã
ã€ãŠãçµæç©äžã«æ®ºèå€ãæ··å ¥ãããããšã«ãã€
ãŠãç §å°ã«ãã€ãŠãŸãã¯å ç±ã«ãã€ãŠæ» èããŠã
ããã ãŸãããããã¯æ» èåºäœçµæç©ã®åœ¢ã§è£œé ããŠ
ãããããããã¯äœ¿çšçŽåã«æ» èæ°ŽãŸãã¯è¥å¹²ã®
ãã®ä»ã®æ³šå°å¯èœãªåªäœã«æº¶è§£ããããšãã§ã
ãã æ¬çºæã®çµæç©äžã®æŽ»æ§æåã®ïŒ ã¯å€ããŠãã
ããããã¯ææã®æ²»çå¹æã«é©ããæäžéãåŸã
ãããããªå²åãæ§æãã¹ãããšãå¿ èŠã§ããã
ããã€ãã®åäœæäžåœ¢æ ã倧äœåæã«æäžããŠã
ããããšã¯æããã§ãããéåžžã泚å°ã«ããæäž
ãå¿ èŠãšãããå Žå補å€ã¯æ®éå°ããšã0.025é
éïŒ ã®æŽ»æ§ç©è³ªãå«æãã¹ãã§ãããçµå£æäžã«
察ããŠã¯è£œå€ã¯æ®éå°ããšã0.1ééïŒ ã®æŽ»æ§ç©
質ãå«æããã§ãããã䜿çšãããæäžéã¯ææ
ã®æ²»çå¹æãæäžçµè·¯ããã³æ²»çã®æç¶ã«å·Šå³ã
ãããæ人ã§ã¯ã现èææã®æ²»çã®ããã®çµå£æ
äžã«ããæçšéã¯0.1ã10ïœïŒæ¥ã§ãã€ãŠããã
ããéåžžïŒãïŒïœïŒæ¥ã§ããã 以äžã®äŸã¯æ¬çºæã«ãã補è¬çµæç©ã説æãã
ãã®ã§ããã äŸ 18 以äžã®çµæãæããé å€ãéåžžã®æè¡ã«ãã€ãŠ
補é ãããã ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã
0.250ïœ ã§ãã·ã 0.150ïœ ã³ãã€ãç¶ã·ãªã« 0.070ïœ ã¹ãã¢ãªã³é žãã°ãã·ãŠã 0.030ïœ äŸ 19 以äžã®çµæãæããé¡ç²ãéåžžã®æè¡ã«ãã€ãŠ
補é ãããã ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã
ïŒ%w/w ãã³ããã 95%w/w ããã«æ¬çºæã®ç¹åŸŽã«ããã°ãççåŠçã«ç¡æ¯
ã®æ äœãšçµåããäžè¬åŒã®å°ããšãïŒçš®ã®åå
ç©ãããªããåç©ããã³é³¥ãžã®æäžã«é©ããçµæ
ç©ïŒãããã®é£ŒæãŸãã¯é£²ææ°Žãžæ·»å ããããã®
æ¿åç©ãå«ããŠïŒãæäŸããããããã§äºããç
çåŠçã«ç¡æ¯ã®æ äœããªãè¡šçŸã¯ãåç©ãŸãã¯é³¥
ã«å®³ã®ãªãæ äœãæå³ãããæ äœã¯åºäœãŸãã¯å
åºäœãŸãã¯æ¶²äœã§ãã€ãŠããããæ äœããã®äžã«
掻æ§ç©è³ªãã»ãã®å°ããã溶解ããªã液äœãäŸã
ã°æ°Žã§ããå Žåãå¿ èŠã«ããä¹³åå€ãåæ£å€ãæž
æ¿å€ãŸãã¯æ¹¿æœ€å€ãçšããŠã掻æ§æåãæ äœäžã«
ç·å¯ã«åæ£ãããŠãã®ãããªçµæç©ã補é ããã®
ã奜éœåã§ããã 奜é©ãªçµæç©ã¯ãã®äžã«æ äœã飌æã«ãã€ãŠå°
ããšãäžéšæäŸãããåºäœãŸãã¯ååºäœããªãã¡
åç©ãŸãã¯é³¥ã«äŸçµŠãããææ©ãŸãã¯é±ç©æ§ç©è³ª
ã§ãããããªãã¡æŽ»æ§æåãåºäœãŸãã¯ååºäœã®
飌æã«æ··å ¥ããŠããããããªãã¡ãæ¬çºæã®å¥ã®
芳ç¹ã¯æå¹éã«ãããŠäžè¬åŒã®å°ããšãïŒçš®ã®
ååç©ãå«æãã飌æã§ããã飌æäžã®æŽ»æ§æå
ã®æ··å ¥ã¯ãæ¹æã転åïŒã¿ã³ããªã³ã°ïŒãŸãã¯ç£š
ç ã®ãããªä»»æã®æ £çšæ³ã«ãã€ãŠè¡ã€ãŠãããã
çš®ã ã®æ¿åºŠã®çµæç©ã掻æ§æåã«å¯Ÿããæ äœã®æ¯
ãå€ããããšã«ãã€ãŠèª¿è£œããããšãã§ããããŸ
ãã掻æ§æåã¯æŽ»æ§æåããã³åºäœãççåŠçã«
ç¡æ¯ã®æ äœäŸãã°å°éºŠã®ç²ç²ãã¿ã«ã¯ãã«ãªãªã³
ãŸãã¯ããšãŒã¯ãŸãã¯ããããåãŸãã¯ãããã®
æ··åç©ãå«æããç²æ«æ¿çž®ç©ã®åœ¢ã§é£Œæã«æ··å ¥ã
ãŠãããããŸãããã®ãããªçµæç©ã¯æ¬çºæã®ç¯
å²å ã«å å«ãããããŸãããããã®çµæç©ã¯æŽ»æ§
æåã®æ äœãžã®äŸãã°å€§è±æ²¹ãžã®ä»çãä¿é²ãã
è¬å€ãå«æããŠããŠãããã飌æãšæ··åããã«å
ç«ã€ãŠã掻æ§æåãŸãã¯ãããå«æããçµæç©
ã«ãïŒçš®ãŸãã¯ãã以äžã®ççåŠçã«ç¡æ¯ã®æ¹¿æœ€
å€ããã³ïŒãŸãã¯ïŒåæ£å€ãäŸãã°Î²âããã¿ãª
ã³ã¹ã«ãã³é žãšãã«ã ã¢ã«ãããã®çž®åçæç©ã
ã©ãŠãªã«ç¡«é žãããªãŠã ãŸãã¯ããªãªãã·ãšãã¬
ã³ïŒ20ïŒãœã«ãã¿ã³ã¢ããªã¬ãšãŒããæ·»å ããŠã
ãããå¥ã®å Žåã湿最å€ãæžæ¿å€ãä¹³åå€ãŸãã¯
åæ£å€ã掻æ§æåãŸãã¯ç²æ«ã«å ããå Žåããã®
ããã«ããŠåŸãããçµæç©ãæ°Žãšæ··åããŠé£Œæã«
æ·»å ããã®ã«é©ããå®å®ãªåæ£ç©ãåŸãŠãããã ãŸãã湿最å€ãæžæ¿å€ãåæ£å€ãŸãã¯ä¹³åå€ã®
å Žåã«ããççåŠçã«ç¡æ¯ã®æ äœãšçµåãã掻æ§
ç©è³ªãããªã飌æãžæ·»å ããã®ã«é©ããçµæç©ã
æ¬çºæã®ç¯å²å ã«å å«ãããã ãŸããæ¬çºæã®çµæç©ã¯ãææã«ãããïŒçš®ãŸ
ãã¯ãã以äžã®äºé²å€ãŸãã¯æ²»çå€äŸãã°æè
å€ãææ§ç©è³ªãé§è«è¬ãæäœè¡åžè«å€ããã³æã³
ã¯ã·ãžãŠã å€ãªãã³ã«æ é€å€äŸãã°ãã¿ãã³ãã
ã³ããã©ã«å¡©ãå«æããŠããŠããããé©åœãªäºé²
å€ãæ²»çå€ããã³æ é€æ·»å å€ã¯åœè©²æè¡ã§ããç¥
ãããŠãããããŠãããäžè¬åŒã®ååç©ããã³
ãã®äžã§çšããããããšããæ¬çºæã®çµæç©ã®ã
ã®ä»ã®æåãšçžå®¹ãåŸããªãã°ææéãã«éžæã
ãŠãããã 液äœçµæç©ã¯é£²ææ°Žäžã®æŽ»æ§æåã®åæ£æ¶²ã§ã
ã€ãŠãããããããŠãããã®çµæç©ã¯æ°Žã«æ·»å ã
ããŠããããããã¯æ°Žãšèªå·±ä¹³åæ§ã§ããæ¿åç©
ãã補é ããŠãããããã®ãããªæ¿åç©ã¯æ¹¿æœ€
å€ãæžæ¿å€ãåæ£å€ãŸãã¯ä¹³åå€å Žåã«ããçç
åŠçã«ç¡æ¯ã®æ äœãšçµåãããããããã¯æ°Žæº¶æ§
ã®ççåŠçã«ç¡æ¯ã®æ äœãšçµåãããã掻æ§æå
ãããªãããããŠæ¬çºæã®ç¯å²å ã«å å«ãããã
ãããã®æ¿åç©ã®äŸã¯æ¬¡ã®éãã§ããã (1) 掻æ§æåãšæ¹¿æœ€å€ãŸãã¯åæ£å€ãšã®æ··åç©ã (2) 掻æ§æåãççåŠçã«ç¡æ¯ã®æ äœã湿最å€ã
æžæ¿å€ãŸãã¯åæ£å€ãããªãç²æ«ã (3) åèš(1)ãŸãã¯(2)ã®æ¿åç©ãæ°Žãšæ··åããŠåŸã
ããå®å®ãªåæ£ç©ããã³ (4) 掻æ§æåãšæ°Žæº¶æ§ã®ççåŠçã«ç¡æ¯ã®æ äœäŸ
ãã°ããç³ãŸãã¯ã°ã«ã³ãŒã¹ãšã®æ··åç©ã ãŸããé©åœãªççåŠçã«ç¡æ¯ã®æ äœããã³è£å©
å€ãšçµåããã掻æ§æåãããªãé¡ç²ããã¬ã
ããæžæ¿æ¶²ã溶液ããã³ä¹³å€ã®åœ¢ã§æ¬çºæã®åå
ç©ãçµå£çã«æäžããããšãã§ãããããããªã
ããã®ãããªæäžã¯éåžžããŸã奜éœåã§ãªãã®
ã§ããã®ãããªçµæç©ã¯å¥œé©ã§ãªãã äžè¬åŒã®ååç©ã¯ã蟲家ãç£å»ããŸãã¯åç©
ããã³é³¥ã®çš®é¡ã幎什ã倧ãããæ§å¥ããã³ç¶æ
ã«é¢å¿ãæãããã®ä»ã®åœæ¥è ã«ãã€ãŠæ±ºå®ãã
ããããªå²åã§éåžžäŸãã°æ¶è²»ããã飌æã®ïŒã
50mg/Kgãè¡šãããããªå²åã§åç©ããã³é³¥ã«æ
äžããããå ã«è¿°ã¹ãããã«ãååç©ã¯é£²ææ°ŽãŸ
ãã¯åºäœãŸãã¯ååºäœã®é£Œæã«ãã€ãŠæäžããŠã
ããã ãã¬ãããŸãã¯é¡ç²ã®åœ¢æ ã®æ¿åç©ãäžè¬åŒ
ã®æŽ»æ§ååç©ã®æäžæ段ãšããŠçšããå Žåããã¬
ãããŸãã¯é¡ç²ããèªäœã«ååšãã掻æ§ååç©ã®
å²åã¯é£Œæäžã«é©ããäžèšã®å²åããããªãé«
ãããããŠæ¿åç©ã飌æå šäœã®å¹³åã§é£œæïŒKgåœ
ãïŒã50mgã®æŽ»æ§ååç©ã®éãäžããããã«é£Œæ
äžã«ååžã§ããããšãç解ãããã§ãããã 以äžã®äŸã¯æ¬çºæã«ããçé·ä¿é²çµæç©ã説æ
ãããã®ã§ããã äŸ 20 è±çšé£Œæçµæç© å€§éºŠ 71Kg ãšããããã 10Kg 倧è±ã±ãŒãïŒèçœè³ª50ïŒ å«æïŒ 15Kg ãã¿ãã³å«æããã©ã«ååç© ïŒKg ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã ïŒïœ
ãããã®çµæã¯äžè¬åŒã®ååç©ãäžè¬åŒã®
ååç©ãšåçšåºŠã®æŽ»æ§ãæããããšã瀺ããŠã
ãã 以äžã®è©Šéšã«ãã€ãŠç€ºãããããã«ãäžè¬åŒ
ã®ååç©ã¯åºä¹³åç©ã«å¯ŸããŠæ¯èŒçç¡å®³ã§ãããš
ããäºå®ã«ãã€ãŠãããã®æçç©è³ªããã³çé·ä¿
é²å€ãšããŠã®æçšæ§ãé«ããããã ããŠã¹æ¯æ§ äžè¬åŒã®ååç©ã®ïŒã€ã«ãã€ãŠããŠã¹ããã
ããçµå£çã«åŠçããããŠæ¬¡ã®ïŒæ¥éãããã芳
å¯ããã åŸãããLD50å€ïŒåŠçãããããŠã¹ã®50ïŒ ã
æ»äº¡ããããéïŒãåç©ã®äœéïŒKgåœãã®mgãšã
ãŠè¡šã«ç€ºããåç©ã®äœéïŒKgåœã1000mgãæäž
ããŠãå®éšã®éããŠã¹ã¯å šç¶æ»äº¡ããªãã€ãã è¡š ååç© LD50  ïŒ1000  ïŒ1000 èšå·ãïŒãã¯æ¬æ现æžã§ã¯ããã倧ããããæ
å³ããã 以äžã®äŸã¯æ¬çºæã«ããæ°èŠååç©ã®è£œé ã説
æãããã®ã§ããã äŸ ïŒ ååç©ïŒ¡ 也ç¥ã¯ãããã«ã ïŒ50mlïŒäžã®ãšãã«ïŒâã¯ã
ããã«ãã«ããããªããŒãïŒ3.2ïœïŒäžã®æº¶æ¶²
ãã也ç¥ã¯ãããã«ã ïŒ100mlïŒäžã®ã¹ãã©ãã€
ã·ã³ïŒ15ïœïŒã®æº¶æ¶²ã«ãããŸããªãã滎å ã
ãã枩床ãæ·»å ã®é氷济äžã§å·åŽããªããïŒâã
10âã®æž©åºŠã«ç¶æãããæ··åç©ã宀枩ã«ïŒæéæŸ
眮ããã溶å€ãç空äžã§é€å»ããããŠæ®çç©ãæ°Ž
æ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒãšäžç·ã«æ¯çªãããåŸ
ãããæ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœ
åºãããæ°Žæ§å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããª
ãŠã 溶液ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽããã次
ã«ãæ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœåº
ããããŠãã®ãšãŒãã«æœåºç©ãç¡«é žãã°ãã·ãŠã
äžã§ä¹Ÿç¥ããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»
ããŠã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ãµã¯ã·ããŒã
ïŒ4.5ïœïŒãçœè²åºäœïŒèç¹100ãã110âïŒã®åœ¢ã§
åŸãã äŸ ïŒ ååç©ïŒ¢ ããªãšãã«ã¢ãã³ïŒïŒïœïŒããã³ä¹Ÿç¥ã¯ããã
ã«ã ïŒ50mlïŒäžã®ãšãã«ïŒâã¯ãããã«ãã«ãã
ã¬ãŒãïŒ5.1ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ãããã«ã
ïŒ100mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ24ïœïŒã®æº¶æ¶²ã«
ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ããã
枩床ã¯æ·»å ã®é氷济äžã§å·åŽããªããïŒâã10â
ã®æž©åºŠã«ç¶æãããæ··åç©ã宀枩ã«ïŒæéæŸçœ®ã
ãã溶å€ãç空äžã§é€å»ããããŠæ®çç©ãæ°Žæ§é ¢
é žæº¶æ¶²ïŒ100mlã1NïŒãšäžç·ã«æ¯çªãããåŸãã
ãæ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœåºã
ããæ°Žæ§å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã
溶液ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽããã次ã«ã
æ··åç©ããžãšãã«ãšãŒãã«ïŒ150mlïŒã§æœåºãã
ããŠãã®ãšãŒãã«æœåºç©ãç¡«é žãã°ãã·ãŠã äžã§
也ç¥ãããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»ã
ãŠã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã
ïŒ3.0ïœïŒãçœè²åºäœïŒèç¹87ãã95âïŒã®åœ¢ã§åŸ
ãã äŸ ïŒ ååç©ïŒ£ ããªãšãã«ã¢ãã³ïŒ1.0ïœïŒããã³ä¹Ÿç¥ã¯ãã
ãã«ã ïŒ10mlïŒäžã®ã¡ãã«ïŒâã¯ãããã«ãã«ã
ãããªããŒãïŒ1.7ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ããã
ã«ã ïŒ100mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®
溶液ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å
ãããæ··åç©ãïŒæéãããŸã次ã«æº¶å€ãç空äž
ã§é€å»ãããåŸãããæ®çç©ã«æ°Žæ§é ¢é žæº¶æ¶²ïŒ75
mlã1NïŒãšãžãšãã«ãšãŒãã«ïŒ100mlïŒãå ã
ããåºäœã溶解ãããŸã§æ··åç©ãæ¯çªãããæ°Žæ§
å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液
ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽããã次ã«æ··åç©
ããžãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºããããŠãš
ãŒãã«æœåºç©ãç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ããã
ãžãšãã«ãšãŒãã«ãæžå§äžã§çå»ããŠã¹ãã©ãã€
ã·ã³ã®2â²âã¡ãã«ãµã€ã¯ã·ããŒãïŒ1.0ïœïŒã
çœè²åºäœïŒèç¹108ãã118âïŒã®åœ¢ã§åŸãã äŸ ïŒ ååç©ïŒ€ 也ç¥ã¯ãããã«ã ïŒ10mlïŒäžã®ãããã«ïŒâã¯
ãããã«ãã«ããããªããŒãïŒ1.8ïœïŒã®æº¶æ¶²ã
也ç¥ã¯ãããã«ã ïŒ70mlïŒäžã®ã¹ãã©ãã€ã·ã³
ïŒ8.4ïœïŒã®æº¶æ¶²ã«ãããŸããªãã滎å ãããæ··å
ç©ãããã«ïŒæéãããŸã次ã«æº¶å€ãç空äžã§é€
å»ãããæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒäž
ã«æº¶è§£ããããŠåŸãããæ··åç©ããžãšãã«ãšãŒã
ã«ïŒ100mlïŒã§æœåºãããæ°Žæ§å±€ãåé¢ããããŠ
æ°Žæ§æ°Žé žåãããªãŠã 溶液ïŒ10NïŒãå ããŠçŽPH
ïŒã«èª¿æŽãããæ··åç©ããžãšãã«ãšãŒãã«ïŒ100
mlïŒã§æœåºããããŠãã®ãšãŒãã«æœåºç©ãç¡«é žã
ã°ãã·ãŠã äžã§ä¹Ÿç¥ãããããžãšãã«ãšãŒãã«ã
æžå§äžã§çå»ããŠã¹ãã©ãã€ã·ã³ã®2â²âããã
ã«ãµã¯ã·ããŒãïŒ0.7ïœïŒãçœè²åºäœïŒèç¹88ã
ã96âïŒã®åœ¢ã§åŸãã äŸ ïŒ ååç©ïŒ¥ ããªãšãã«ã¢ãã³ïŒ1.0ïœïŒããã³ä¹Ÿç¥ã¯ãã
ãã«ã ïŒ20mlïŒäžã®ã¡ãã«ïŒâã¯ãããã«ãã«ã
ãã¬ãŒãïŒ1.65ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ãããã«ã
ïŒ70mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶æ¶²ã«
ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ããã
æ··åç©ãããã«ïŒæéãããŸããããŠ17æéæŸçœ®
ãããåå¿æ··åç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒ
ã§æœåºãããã¯ãããã«ã å±€ãåé¢ããããŠïŒ%
ïœïŒïœæ°Žæ§çé žãããªãŠã 溶液ïŒ100mlïŒã§æŽæµã
ãã次ã«ãã¯ãããã«ã ãç¡«é žãã°ãã·ãŠã äžã§
也ç¥ããããã¯ãããã«ã ãæžå§äžã§çå»ããŠã¹
ãã©ãã€ã·ã³ã®2â²âã¡ãã«ã°ã«ã¿ã¬ãŒãïŒ3.2
ïœïŒãé»è€è²åºäœïŒèç¹85ãã95âïŒã®åœ¢ã§åŸ
ãã äŸ ïŒ ååç©ïŒŠ éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ40mlïŒäžã®ã¡ãã«ãã©ã³ã¹âïŒâã¯ãããã«ã
ã«ã¢ã¯ãªã¬ãŒãïŒ2.2ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»ã
ã³ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶
液ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ã
ããæ··åç©ãããã«ïŒæéãããŸãã17æéæŸçœ®
ã次ã«åºäœãå»ããã液ãèžçºãããããŠæ®
çç©ãé ¢é žãšãã«ïŒ100mlïŒäžã«æº¶è§£ããããã
ã®æº¶æ¶²ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã1NïŒã§æœåºã
ããæ°Žæ§å±€ãåé¢ããããŠã¯ãããã«ã ïŒïŒÃ50
mlïŒã§æœåºãããäžç·ã«ããã¯ãããã«ã æœåºç©
ãæ°ŽïŒ100mlïŒïŒ%w/væ°Žæ§éçé žãããªãŠã 溶
液ïŒ100mlïŒã§æŽæµããã次ã«ãã¯ãããã«ã å±€
ãç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ããããã¯ãããã«
ã ãæžå§äžã§çå»ããŠã¹ãã©ãã€ã·ã³ã®2â²âã¡
ãã«ããã¬ãŒãïŒ2.7ïœïŒïŒèç¹105ãã115âïŒã
åŸãã äŸ ïŒ ååç©ïŒ§ ããªãšãã«ã¢ãã³ïŒ1.1ïœïŒããã³ãšãã«ã¯ã
ããã«ã¡ãŒãïŒ1.1ïœïŒããã³ä¹Ÿç¥ã¯ãããã«ã
ïŒ50mlïŒäžã®ã¡ã¿ã³ããªãŒã«ïŒ1.0ïœïŒã®æº¶æ¶²ãã
â15âã«å·åŽããã也ç¥ã¯ãããã«ã ïŒ80mlïŒäž
ã®ã¹ãã©ãã€ã·ã³2â²âãããµã¯ã·ããŒãïŒ9.4
ïœïŒã®æº¶æ¶²ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Ž
ã¥ã€å ãããæ·»å ã®éã枩床ãâ15âãªããâ10
âã®æž©åºŠã«ç¶æãããæ··åç©ã宀枩ãŸã§å æž©ãã
ããŠ18æéæŸçœ®ããã溶å€ãæžå§äžã§èžçã«ãã€
ãŠé€å»ããããžãšãã«ãšãŒãã«ïŒ100mlïŒãæ®ç
ç©ã«å ããããŠäžæº¶æ§ç©è³ªãå»ãããéç©ã
æ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒã§æœåºãããæ°Žæ§å±€
ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液
ïŒ10NïŒãå ããŠçŽPHïŒã«èª¿æŽãããæ··åç©ããž
ãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºããããŠãžãšã
ã«ãšãŒãã«æœåºç©ãïŒ%w/væ°Žæ§éçé žãããªãŠ
ã 溶液ïŒ100mlïŒã§æŽæµããç¡«é žãã°ãã·ãŠã äž
ã§ä¹Ÿç¥ãããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»
ããŠã¹ãã©ãã€ã·ã³ã®2â²ââã¡ãã«ããªãµã¯
ã·ããŒãïŒ1.4ïœïŒïŒèç¹114ãã124âïŒãåŸãã åèšã®è£œé äŸã§çšããããã¹ãã©ãã€ã·ã³ã®
2â²âãã¯ãé žãããšã¹ãã«ã次ã®ããã«ããŠè£œé
ããã ç¡æ°Žãã¯ãé žïŒ6.0ïœïŒãé ¢é žãšãã«ïŒ600mlïŒ
äžã®ã¹ãã©ãã€ã·ã³ïŒ50.4ïœïŒã®æº¶æ¶²ã«ãããŸ
ããªããå ãããæ··åç©ãããã«ïŒæéãããŸã
ãããŠå®€æž©ã«18æéæŸçœ®ããã溶å€ãç空äžã§é€
å»ããããŠãžãšãã«ãšãŒãã«ïŒ200mlïŒãæ®çç©
ã«å ãããåºäœãå»ã也ç¥ãããŠã¹ãã©ãã€ã·
ã³ã®2â²âãã¯ãé žãããšã¹ãã«ïŒ38.6ïœïŒïŒè
ç¹140ãã148âïŒãåŸãã äŸ ïŒ ååç©ïŒš éçé žãããªãŠã ïŒ4.0ïœïŒããã³ä¹Ÿç¥ã¢ã»ã
ã³ïŒ10mlïŒäžã®ã·ã¯ãããã·ã«ïŒâã¯ãããã«ã
ã«ããããªããŒãïŒ2.4ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»
ãã³ïŒ70mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®
溶液ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å
ãããæ··åç©ãïŒæéãããŸã次ã«16æéæŸçœ®ã
ããæ··åç©ãéããããŠæ¶²ãæžå§äžã§èžçºã
ãããæ®çç©ãé ¢é žãšãã«ïŒ70mlïŒäžã«æº¶è§£ãã
ããŠåŸãããæ··åç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã
0.5NïŒã§æœåºãããæ°Žæ§å±€ãåé¢ããããŠã¯ã
ããã«ã ïŒïŒÃ50mlïŒã§æœåºãããäžç·ã«ããã¯
ãããã«ã æœåºç©ãæ°ŽïŒ100mlïŒãšïŒ%w/væ°Žæ§
éçé žãããªãŠã 溶液ïŒ75mlïŒã§æŽæµãããããŠ
ç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ããããã¯ãããã«ã
ãç空äžã§èžçºé€å»ããŠã¹ãã©ãã€ã·ã³ã®2â²â
ã·ã¯ãããã·ã«ãµã¯ã·ããŒãïŒ3.4ïœïŒïŒèç¹90ã
ã100âïŒãåŸãã äŸ ïŒ ååç©ïŒª éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ20mlïŒäžã®ãšãã«ïŒâã¯ãããã«ãã«ã¢ã»ããŒ
ãïŒ1.5ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»ãã³ïŒ75mlïŒäž
ã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶æ¶²ã«ãããŸã
ãªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ãããæ¹æãïŒ
æéç¶ã次ã«æ··åç©ã17æéæŸçœ®ãããåºäœã
å»ããããŠæ¶²ãç空äžã§èžçºããããæ®çç©ã
é ¢é žãšãã«ïŒ100mlïŒäžã«æº¶è§£ããããŠãã®æº¶æ¶²
ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã0.5NïŒã§æœåºãããæ°Ž
æ§å±€ãåé¢ããããŠã¯ãããã«ã ïŒïŒÃ50mlïŒã§
æœåºãããã¯ãããã«ã æœåºç©ãäžç·ã«ããæ°Ž
ïŒ100mlïŒããã³ïŒ%w/væ°Žæ§éçé žãããªãŠã 溶
液ïŒ100mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äž
ã§ä¹Ÿç¥ããããã¯ãããã«ã ãæžå§äžã§çå»ããŠ
ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ããããŒãïŒ2.0
ïœïŒïŒèç¹89ãã99âïŒãåŸãã äŸ 10 ååç©ïŒ« ããªãšãã«ã¢ãã³ïŒ1.5ïœïŒããã³ãšãã«ã¯ã
ããã«ã¡ãŒãïŒ1.7ïœïŒããâ15âã«å·åŽããã也
ç¥ã¯ãããã«ã ïŒ50mlïŒäžã®ããšãã«æ°ŽçŽ ãµã¯ã·
ããŒãïŒ2.7ïœïŒã®æº¶æ¶²ã«ãããŸããªããå ã
ãã也ç¥ã¯ãããã«ã ïŒ30mlïŒäžã®ã¹ãã©ãã€ã·
ã³ïŒ8.4ïœïŒã®æº¶æ¶²ãåŸãããæ··åç©äžã«æ»Žå
ãããããšåæã«æ··åç©ã®æž©åºŠãâ15âãªããâ
10âã«ç¶æãããæ··åç©ã宀枩ãŸã§å æž©ããããŠ
17æéæŸçœ®ãããæ··åç©ãç空äžã§èžçºãããã
ããŠé ¢é žãšãã«ïŒ75mlïŒãšïŒ%w/væ°Žæ§éçé žã
ããªãŠã 溶液ïŒ75mlïŒãæ®çç©ã«å ãããææ©å±€
ãåé¢ããããŠæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã0.5NïŒã§
æœåºããã次ã«ãæ°Žæ§å±€ãã¯ãããã«ã ïŒïŒÃ50
mlïŒã§æœåºãããäžç·ã«ããã¯ãããã«ã æœåºç©
ãæ°ŽïŒ100mlïŒãšïŒ%w/væ°Žæ§éçé žãããªãŠã
溶液ïŒ75mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äž
ã§ä¹Ÿç¥ããããæžå§äžã§ã¯ãããã«ã ãèžçºãã
ãŠã¹ãã©ãã€ã·ã³ã®2â²âããšãã«ãµã¯ã·ããŒã
ïŒ5.1ïœïŒïŒèç¹105ãã115âïŒãåŸãã äŸ 11 ååç©ïŒ¬ éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ20mlïŒäžã®ã¡ãã«ïŒRSïŒâïŒâã¯ãããã«ãã«
âïŒâã¡ãã«ããããªããŒãïŒ2.5ïœïŒã®æº¶æ¶²
ãã也ç¥ã¢ã»ãã³ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³
ïŒ8.4ïœïŒã®æº¶æ¶²ã«ãããŸããªããå ãããåŸè ã¯
ïŒæ»Žã¥ã€å ããããããŸããïŒæéç¶ã次ã«æ··å
ç©ã18æéæŸçœ®ãããåºäœãå»ããããŠæ¶²ã
ç空äžã§èžçºããããæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75
mlã0.5NïŒäžã«æº¶è§£ãããåŸãããæ··åç©ãé ¢
é žãšãã«ïŒïŒÃ50mlïŒãããŠæ¬¡ã«ã¯ãããã«ã
ïŒïŒÃ50mlïŒã§æœåºãããäžç·ã«ããã¯ãããã«
ã æœåºç©ãæ°ŽïŒ100mlïŒããã³ïŒ%w/væ°Žæ§éç
é žãããªãŠã 溶液ïŒ100mlïŒã§æŽæµããããŠç¡«é ž
ãã°ãã·ãŠã äžã§ä¹Ÿç¥ããããã¯ãããã«ã ãæž
å§äžã§çå»ããŠã¹ãã©ãã€ã·ã³ã®2â²âã¡ãã«
ïŒRSïŒâïŒïŒâã¡ãã«ïŒãµã¯ã·ããŒãïŒ1.7ïœïŒïŒè
ç¹92ãã102âïŒãåŸãã äŸ 12 ååç©ïŒ ããªãšãã«ã¢ãã³ïŒ1.1ïœïŒããã³ãšãã«ã¯ã
ããã«ã ã¡ãŒãïŒ1.1ïœïŒããâ15âã«å·åŽããã
也ç¥ã¯ãããã«ã ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³
2â²âãããµã¯ã·ããŒãïŒ9.4ïœïŒã®æº¶æ¶²ã«ãããŸ
ããªããå ããã枩床ãâ15âãªããâ10âã«ç¶
æããªããã也ç¥ã¯ãããã«ã ïŒ20mlïŒäžã®ïŒâ
ããšãã«ãšã¿ããŒã«ïŒ1.8ïœïŒã®æº¶æ¶²ã滎å ã
ãã次ã«ãæ··åç©ã宀枩ãŸã§å æž©ããããŠ18æé
æŸçœ®ããã溶å€ãæžå§äžã§é€å»ããããŠæ®çç©ã
æ°Žæ§é ¢é žæº¶æ¶²ïŒ100mlã1NïŒäžã«æº¶è§£ããã溶液
ããžãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºãããæ°Žæ§
å±€ãåé¢ããããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液
ïŒ10NïŒã§çŽPHïŒã«èª¿æŽãããæ··åç©ããžãšãã«
ãšãŒãã«ïŒ100mlïŒã§æœåºããããŠãã®ãšãŒãã«
æœåºç©ãïŒ%w/væ°Žæ§éçé žãããªãŠã 溶液
ïŒ100mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿ
ç¥ãããããžãšãã«ãšãŒãã«ãæžå§äžã§çå»ããŠ
ã¹ãã©ãã€ã·ã³ã®2â²âïŒïŒâããšãã«ãšãã«ïŒ
ãµã¯ã·ããŒãïŒ2.3ïœïŒïŒç¹58ã68âïŒãåŸãã äŸ 13 ååç©ïŒ® ããªãšãã«ã¢ãã³ïŒ1.1ïœïŒããã³ãšãã«ã¯ã
ããã«ã¡ãŒãïŒ1.1ïœïŒãâ15âã«å·åŽããã也
ç¥ã¯ãããã«ã ïŒ80mlïŒäžã®ã¹ãã©ãã€ã·ã³
2â²âãããµã¯ã·ããŒãïŒ9.4ïœïŒã®æº¶æ¶²ã«ãããŸ
ããªããå ããã次ã«ã枩床ãâ15âãªããâ10
âã«ç¶æããªããã也ç¥ã¯ãããã«ã ïŒ40mlïŒäž
ã®ç¬¬ïŒãã¿ããŒã«ïŒ1.2mlïŒã®æº¶æ¶²ãåŸãããæ··
åç©ã«æ»Žå ãããåå¿ç©ã宀枩ãŸã§æž©ããªããã
ãããŸããç¶ã次ã«æ··åç©ã18æéæŸçœ®ããã溶
å€ãæžå§äžã§é€å»ããããŠæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²
ïŒ100mlã1NïŒäžã«æº¶è§£ãããæ··åç©ããžãšãã«
ãšãŒãã«ïŒ100mlïŒã§æœåºãããæ°Žæ§å±€ãåé¢ã
ãããŠæ°Žæ§æ°Žé žåãããªãŠã 溶液ïŒ10NïŒã§çŽPH
ïŒã«èª¿æŽãããæ··åç©ããžãšãã«ãšãŒãã«ïŒ100
mlïŒã§æœåºããããŠãã®ãšãŒãã«æœåºç©ãïŒ%
ïœïŒïœæ°Žæ§éçé žãããªãŠã 溶液ïŒ100mlïŒã§æŽæµ
ããããŠç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ãããããžãš
ãã«ãšãŒãã«ãæžå§äžã§çå»ããŠã¹ãã©ãã€ã·ã³
ã®2â²â第ïŒããã«ãµã¯ã·ããŒãïŒ0.9ïœïŒïŒèç¹
99ãã108âïŒãåŸãã äŸ 14 ååç©ïŒ¯ 也ç¥ã¯ãããã«ã ïŒ10mlïŒäžã®ããªãšãã«ã¢ã
ã³ïŒ1.0ïœïŒã®æº¶æ¶²ããã³ä¹Ÿç¥ã¯ãããã«ã ïŒ200
mlïŒäžã®ã€ãœãããã«ïŒâã¯ãããã«ãã«ããã
ãªããŒãïŒ2.2ïœïŒã®æº¶æ¶²ãã也ç¥ã¯ãããã«ã
ïŒ75mlïŒäžã®ã¹ãã©ãã€ã·ã³ïŒ8.4ïœïŒã®æº¶æ¶²ã«
ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ããã
æ··åç©ãïŒæéãããŸã次ã«18æéæŸçœ®ããã溶
å€ãæžå§äžã§é€å»ãããããŠæ°Žæ§é ¢é žæº¶æ¶²ïŒ100
mlã1NïŒããã³ãžãšãã«ãšãŒãã«ïŒ100mlïŒãæ¯
çªãããŠå ãããæ°Žæ§å±€ãåé¢ããããŠæ°Žæ§æ°Žé ž
åãããªãŠã 溶液ïŒ10NïŒã§çŽPHïŒã«èª¿æŽããã
æ··åç©ããžãšãã«ãšãŒãã«ïŒ100mlïŒã§æœåºãã
ããŠãã®ãšãŒãã«æœåºç©ãïŒ%w/væ°Žæ§éçé žã
ããªãŠã 溶液ïŒ100mlïŒã§æŽæµããããŠç¡«é žãã°
ãã·ãŠã äžã§ä¹Ÿç¥ãããããžãšãã«ãšãŒãã«ãæž
å§äžã§é€å»ããŠã¹ãã©ãã€ã·ã³ã®2â²âã€ãœãã
ãã«ãµã¯ã·ããŒãïŒ1.2ïœïŒïŒèç¹105ãã115âïŒ
ãåŸãã äŸ 15 ååç©ïŒ° éçé žãããªãŠã ïŒïŒïœïŒããã³ä¹Ÿç¥ã¢ã»ãã³
ïŒ30mlïŒäžã®ãšãã«ïŒâã¯ãããã«ãã«ããããª
ããŒãïŒ2.5ïœïŒã®æº¶æ¶²ãã也ç¥ã¢ã»ãã³ïŒ80
mlïŒäžã®ã¹ãã©ãã€ã·ã³æ··åç©ïŒ8.4ïœïŒã®æº¶æ¶²
ã«ãããŸããªããå ãããåŸè ã¯ïŒæ»Žã¥ã€å ã
ããæ··åç©ãïŒæéãããŸã次ã«18æéæŸçœ®ã
ããåºäœãå»ããããŠæ¶²ãæžå§äžã§èžçºãã
ããæ®çç©ãæ°Žæ§é ¢é žæº¶æ¶²ïŒ75mlã0.5NïŒäžã«
溶解ããããŠåŸãããæ··åç©ãé ¢é žãšãã«ïŒïŒÃ
50mlïŒæ¬¡ã«ã¯ãããã«ã ïŒïŒÃ50mlïŒã§æœåºã
ããäžç·ã«ããã¯ãããã«ã æœåºç©ãæ°ŽïŒ100
mlïŒãšïŒ%w/væ°Žæ§éçé žãããªãŠã 溶液ïŒ100
mlïŒã§æŽæµããããŠç¡«é žãã°ãã·ãŠã äžã§ä¹Ÿç¥ã
ãããã¯ãããã«ã ãæžå§äžã§é€å»ããŠã¹ãã©ã
ã€ã·ã³æ··åç©ã®2â²âãšãã«ãµã¯ã·ããŒãïŒ4.4
ïœïŒïŒèç¹80ãã90âïŒãåŸãã åèšã®è£œé äŸã§çšããããã¹ãã©ãã€ã·ã³æ··å
ç©ã¯ãçŽ60%w/vã¹ãã©ãã€ã·ã³ã20%w/wã¹
ãã©ãã€ã·ã³ããã³20%w/wã¹ãã©ãã€ã·ã³
ãããªããã®ã§ãã€ãã äŸ 16 ååç©ïŒ± ãšã¿ããŒã«äžã®å¡©åæ°ŽçŽ ã®æº¶æ¶²ãããã¯ãåºäœ
ãæ²æ®¿ããªããªããŸã§ãžãšãã«ãšãŒãã«ïŒ50mlïŒ
äžã®ã¹ãã©ãã€ã·ã³ïŒ1.5ïœïŒã®2â²âãšãã«ãµ
ã¯ã·ããŒãã®æº¶æ¶²ã«æ»Žå ãããçœè²åºäœãå»
ãããžãšãã«ãšãŒãã«ã§æŽæµããããŠä¹Ÿç¥ãããŠ
ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ãµã¯ã·ããŒãã®äº
å¡©é žå¡©ïŒ1.4ïœïŒïŒèç¹64ãã74âïŒãåŸãã äŸ 17 ååç©ïŒ² ãžãšãã«ãšãŒãã«äžã®ãããé žã®é£œå溶液ãã
ãã¯ãåºäœãæ²æ®¿ããªããªããŸã§ããžãšãã«ãšãŒ
ãã«ïŒ50mlïŒäžã®ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«
ãµã¯ã·ããŒãïŒ1.5ïœïŒã®æº¶æ¶²ã«æ»Žå ãããçœè²
åºäœãå»ãããžãšãã«ãšãŒãã«ã§æŽæµããããŠ
也ç¥ãããŠã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ãµã¯ã·
ããŒãã®äºãããé žå¡©ïŒ1.6ïœïŒïŒèç¹85ãã97
âïŒãåŸãã æ¬çºæã¯çžå®¹æ§ã®è£œè¬çã«èš±å®¹ãåŸãæ äœãŸã
ã¯å€ç®ãšå ±ã«äžè¬åŒã®å°ããšãïŒçš®ã®ååç©ã
å«æãã補è¬æçç©çµæç©ããã®ç¯å²å ã«å å«ã
ããäžè¬åŒã®ååç©ã¯ãçŽè žå ã«ãè å ã«ãé
çµå£çã«ããã«ç¹ã«ã¯çµå£çã«æäžãããã®ãæ®
éã§ããã çµå£æäžçšã®åºäœçµæç©ã¯å§çž®é å€ããã«ãå
æ£æ§ç²æ«ããã³é¡ç²ãå å«ããããã®ãããªåºäœ
çµæç©äžã§ãïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§ååç©
ã¯ããã³ããããçé žã«ã«ã·ãŠã ããããããã§
ãã·ããã¢ã«ã®ã³é žãŸãã¯ä¹³ç³ã®ãããªå°ããšã
ïŒçš®ã®äžæŽ»æ§åžéå€ãšæ··åãããããŸããéåžžå®
æœãããããã«çµæç©ã¯äžæŽ»æ§åžéå€ä»¥å€ã®è¿œå
ã®ç©è³ªäŸãã°ã¹ãã¢ãªã³é žãã°ãã·ãŠã ã®ãããª
æ»å€ãããªã€ãŠããŠããããçµå£æäžçšã®æ¶²äœçµ
æç©ã¯ã補è¬çã«èš±å®¹ãåŸãä¹³å€ã溶液ãæžæ¿
液ãã·ãããããã³åœæè¡åéã§æ®éã«çšããã
ãäžæŽ»æ§åžéå€äŸãã°æ°Žããã³æ¶²äœãã©ãã€ã³ã
å«æãããšãªãã·ã«ãå å«ãããäžæŽ»æ§åžéå€ä»¥
å€ã«ããŸããã®ãããªçµæç©ã¯è£å©å€äŸãã°æ¹¿æœ€
å€ãæžæ¿å€ãçå³å€ãéŠå³å€ãéŠæããã³ä¿åå€
ãããªã€ãŠããŠãããããŸããæ¬çºæã«ããçµå£
æäžçšã®çµæç©ã¯ãåžéå€ãŸãã¯è³Šåœ¢å€ãæ·»å ãŸ
ãã¯æ·»å ããŠããªãïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§ç©
質ãå«æãããŒã©ãã³ã®ãããªåžåæ§ç©è³ªã®ã«ã
ã»ã«ãå å«ããã çµå£æäžçšã®çµæç©ã¯ãããèªäœæ¢ç¥ã®æ¹æ³
ïŒãã®ãããªæ¹æ³ã¯ãããŸã§çšããããæ¹æ³ãŸã
ã¯æç®ã«èšèŒãããæ¹æ³ãšå®çŸ©ãããïŒã§èª¿åã
ããé¡ç²ïŒããã¯äœ¿çšåã«æ°Žäžã«åæ£ãããŠããŠ
ãããïŒãå å«ããã è å æäžçšã®åºäœçµæç©ã¯ããèªäœæ¢ç¥ã®æ¹æ³
ã§èª¿åãããããŠïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§åå
ç©ãå«æãããããµãªãŒãå å«ããã çŽè žå æäžçšã®åºäœçµæç©ã¯ããèªäœæ¢ç¥ã®æ¹
æ³ã§èª¿åãããããŠïŒçš®ãŸãã¯ãã以äžã®æŽ»æ§å
åç©ãå«æããåè¬ãå å«ããã éçµå£æäžçšã®æ¬çºæã«ãã補å€ã¯æ» èæ°Žæ§ãŸ
ãã¯éæ°Žæ§æº¶æ¶²ãæžæ¿æ¶²ãŸãã¯ä¹³å€ãå å«ããã
éæ°Žæ§æº¶å€ãŸãã¯æžæ¿åªäœã®äŸã¯ããããã¬ã³ã°
ãªã³ãŒã«ãããªãšãã¬ã³ã°ãªã³ãŒã«ããªãªãŒãæ²¹
ã®ãããªæ€ç©æ²¹ããã³ãªã¬ã€ã³é žãšãã«ã®ãããª
泚å°å¯èœãªææ©ãšã¹ãã«ã§ããããŸãããããã®
çµæç©ã¯è£å©å€äŸãã°ä¿åå€ã湿最å€ãä¹³åå€ã
ãã³åæ£å€ãå å«ããŠããŠãããããããã¯äŸã
ã°ãã¯ããªã¢ä¿æãã€ã«ã¿ãŒãéããŠã®éã«ã
ã€ãŠãçµæç©äžã«æ®ºèå€ãæ··å ¥ãããããšã«ãã€
ãŠãç §å°ã«ãã€ãŠãŸãã¯å ç±ã«ãã€ãŠæ» èããŠã
ããã ãŸãããããã¯æ» èåºäœçµæç©ã®åœ¢ã§è£œé ããŠ
ãããããããã¯äœ¿çšçŽåã«æ» èæ°ŽãŸãã¯è¥å¹²ã®
ãã®ä»ã®æ³šå°å¯èœãªåªäœã«æº¶è§£ããããšãã§ã
ãã æ¬çºæã®çµæç©äžã®æŽ»æ§æåã®ïŒ ã¯å€ããŠãã
ããããã¯ææã®æ²»çå¹æã«é©ããæäžéãåŸã
ãããããªå²åãæ§æãã¹ãããšãå¿ èŠã§ããã
ããã€ãã®åäœæäžåœ¢æ ã倧äœåæã«æäžããŠã
ããããšã¯æããã§ãããéåžžã泚å°ã«ããæäž
ãå¿ èŠãšãããå Žå補å€ã¯æ®éå°ããšã0.025é
éïŒ ã®æŽ»æ§ç©è³ªãå«æãã¹ãã§ãããçµå£æäžã«
察ããŠã¯è£œå€ã¯æ®éå°ããšã0.1ééïŒ ã®æŽ»æ§ç©
質ãå«æããã§ãããã䜿çšãããæäžéã¯ææ
ã®æ²»çå¹æãæäžçµè·¯ããã³æ²»çã®æç¶ã«å·Šå³ã
ãããæ人ã§ã¯ã现èææã®æ²»çã®ããã®çµå£æ
äžã«ããæçšéã¯0.1ã10ïœïŒæ¥ã§ãã€ãŠããã
ããéåžžïŒãïŒïœïŒæ¥ã§ããã 以äžã®äŸã¯æ¬çºæã«ãã補è¬çµæç©ã説æãã
ãã®ã§ããã äŸ 18 以äžã®çµæãæããé å€ãéåžžã®æè¡ã«ãã€ãŠ
補é ãããã ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã
0.250ïœ ã§ãã·ã 0.150ïœ ã³ãã€ãç¶ã·ãªã« 0.070ïœ ã¹ãã¢ãªã³é žãã°ãã·ãŠã 0.030ïœ äŸ 19 以äžã®çµæãæããé¡ç²ãéåžžã®æè¡ã«ãã€ãŠ
補é ãããã ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã
ïŒ%w/w ãã³ããã 95%w/w ããã«æ¬çºæã®ç¹åŸŽã«ããã°ãççåŠçã«ç¡æ¯
ã®æ äœãšçµåããäžè¬åŒã®å°ããšãïŒçš®ã®åå
ç©ãããªããåç©ããã³é³¥ãžã®æäžã«é©ããçµæ
ç©ïŒãããã®é£ŒæãŸãã¯é£²ææ°Žãžæ·»å ããããã®
æ¿åç©ãå«ããŠïŒãæäŸããããããã§äºããç
çåŠçã«ç¡æ¯ã®æ äœããªãè¡šçŸã¯ãåç©ãŸãã¯é³¥
ã«å®³ã®ãªãæ äœãæå³ãããæ äœã¯åºäœãŸãã¯å
åºäœãŸãã¯æ¶²äœã§ãã€ãŠããããæ äœããã®äžã«
掻æ§ç©è³ªãã»ãã®å°ããã溶解ããªã液äœãäŸã
ã°æ°Žã§ããå Žåãå¿ èŠã«ããä¹³åå€ãåæ£å€ãæž
æ¿å€ãŸãã¯æ¹¿æœ€å€ãçšããŠã掻æ§æåãæ äœäžã«
ç·å¯ã«åæ£ãããŠãã®ãããªçµæç©ã補é ããã®
ã奜éœåã§ããã 奜é©ãªçµæç©ã¯ãã®äžã«æ äœã飌æã«ãã€ãŠå°
ããšãäžéšæäŸãããåºäœãŸãã¯ååºäœããªãã¡
åç©ãŸãã¯é³¥ã«äŸçµŠãããææ©ãŸãã¯é±ç©æ§ç©è³ª
ã§ãããããªãã¡æŽ»æ§æåãåºäœãŸãã¯ååºäœã®
飌æã«æ··å ¥ããŠããããããªãã¡ãæ¬çºæã®å¥ã®
芳ç¹ã¯æå¹éã«ãããŠäžè¬åŒã®å°ããšãïŒçš®ã®
ååç©ãå«æãã飌æã§ããã飌æäžã®æŽ»æ§æå
ã®æ··å ¥ã¯ãæ¹æã転åïŒã¿ã³ããªã³ã°ïŒãŸãã¯ç£š
ç ã®ãããªä»»æã®æ £çšæ³ã«ãã€ãŠè¡ã€ãŠãããã
çš®ã ã®æ¿åºŠã®çµæç©ã掻æ§æåã«å¯Ÿããæ äœã®æ¯
ãå€ããããšã«ãã€ãŠèª¿è£œããããšãã§ããããŸ
ãã掻æ§æåã¯æŽ»æ§æåããã³åºäœãççåŠçã«
ç¡æ¯ã®æ äœäŸãã°å°éºŠã®ç²ç²ãã¿ã«ã¯ãã«ãªãªã³
ãŸãã¯ããšãŒã¯ãŸãã¯ããããåãŸãã¯ãããã®
æ··åç©ãå«æããç²æ«æ¿çž®ç©ã®åœ¢ã§é£Œæã«æ··å ¥ã
ãŠãããããŸãããã®ãããªçµæç©ã¯æ¬çºæã®ç¯
å²å ã«å å«ãããããŸãããããã®çµæç©ã¯æŽ»æ§
æåã®æ äœãžã®äŸãã°å€§è±æ²¹ãžã®ä»çãä¿é²ãã
è¬å€ãå«æããŠããŠãããã飌æãšæ··åããã«å
ç«ã€ãŠã掻æ§æåãŸãã¯ãããå«æããçµæç©
ã«ãïŒçš®ãŸãã¯ãã以äžã®ççåŠçã«ç¡æ¯ã®æ¹¿æœ€
å€ããã³ïŒãŸãã¯ïŒåæ£å€ãäŸãã°Î²âããã¿ãª
ã³ã¹ã«ãã³é žãšãã«ã ã¢ã«ãããã®çž®åçæç©ã
ã©ãŠãªã«ç¡«é žãããªãŠã ãŸãã¯ããªãªãã·ãšãã¬
ã³ïŒ20ïŒãœã«ãã¿ã³ã¢ããªã¬ãšãŒããæ·»å ããŠã
ãããå¥ã®å Žåã湿最å€ãæžæ¿å€ãä¹³åå€ãŸãã¯
åæ£å€ã掻æ§æåãŸãã¯ç²æ«ã«å ããå Žåããã®
ããã«ããŠåŸãããçµæç©ãæ°Žãšæ··åããŠé£Œæã«
æ·»å ããã®ã«é©ããå®å®ãªåæ£ç©ãåŸãŠãããã ãŸãã湿最å€ãæžæ¿å€ãåæ£å€ãŸãã¯ä¹³åå€ã®
å Žåã«ããççåŠçã«ç¡æ¯ã®æ äœãšçµåãã掻æ§
ç©è³ªãããªã飌æãžæ·»å ããã®ã«é©ããçµæç©ã
æ¬çºæã®ç¯å²å ã«å å«ãããã ãŸããæ¬çºæã®çµæç©ã¯ãææã«ãããïŒçš®ãŸ
ãã¯ãã以äžã®äºé²å€ãŸãã¯æ²»çå€äŸãã°æè
å€ãææ§ç©è³ªãé§è«è¬ãæäœè¡åžè«å€ããã³æã³
ã¯ã·ãžãŠã å€ãªãã³ã«æ é€å€äŸãã°ãã¿ãã³ãã
ã³ããã©ã«å¡©ãå«æããŠããŠããããé©åœãªäºé²
å€ãæ²»çå€ããã³æ é€æ·»å å€ã¯åœè©²æè¡ã§ããç¥
ãããŠãããããŠãããäžè¬åŒã®ååç©ããã³
ãã®äžã§çšããããããšããæ¬çºæã®çµæç©ã®ã
ã®ä»ã®æåãšçžå®¹ãåŸããªãã°ææéãã«éžæã
ãŠãããã 液äœçµæç©ã¯é£²ææ°Žäžã®æŽ»æ§æåã®åæ£æ¶²ã§ã
ã€ãŠãããããããŠãããã®çµæç©ã¯æ°Žã«æ·»å ã
ããŠããããããã¯æ°Žãšèªå·±ä¹³åæ§ã§ããæ¿åç©
ãã補é ããŠãããããã®ãããªæ¿åç©ã¯æ¹¿æœ€
å€ãæžæ¿å€ãåæ£å€ãŸãã¯ä¹³åå€å Žåã«ããçç
åŠçã«ç¡æ¯ã®æ äœãšçµåãããããããã¯æ°Žæº¶æ§
ã®ççåŠçã«ç¡æ¯ã®æ äœãšçµåãããã掻æ§æå
ãããªãããããŠæ¬çºæã®ç¯å²å ã«å å«ãããã
ãããã®æ¿åç©ã®äŸã¯æ¬¡ã®éãã§ããã (1) 掻æ§æåãšæ¹¿æœ€å€ãŸãã¯åæ£å€ãšã®æ··åç©ã (2) 掻æ§æåãççåŠçã«ç¡æ¯ã®æ äœã湿最å€ã
æžæ¿å€ãŸãã¯åæ£å€ãããªãç²æ«ã (3) åèš(1)ãŸãã¯(2)ã®æ¿åç©ãæ°Žãšæ··åããŠåŸã
ããå®å®ãªåæ£ç©ããã³ (4) 掻æ§æåãšæ°Žæº¶æ§ã®ççåŠçã«ç¡æ¯ã®æ äœäŸ
ãã°ããç³ãŸãã¯ã°ã«ã³ãŒã¹ãšã®æ··åç©ã ãŸããé©åœãªççåŠçã«ç¡æ¯ã®æ äœããã³è£å©
å€ãšçµåããã掻æ§æåãããªãé¡ç²ããã¬ã
ããæžæ¿æ¶²ã溶液ããã³ä¹³å€ã®åœ¢ã§æ¬çºæã®åå
ç©ãçµå£çã«æäžããããšãã§ãããããããªã
ããã®ãããªæäžã¯éåžžããŸã奜éœåã§ãªãã®
ã§ããã®ãããªçµæç©ã¯å¥œé©ã§ãªãã äžè¬åŒã®ååç©ã¯ã蟲家ãç£å»ããŸãã¯åç©
ããã³é³¥ã®çš®é¡ã幎什ã倧ãããæ§å¥ããã³ç¶æ
ã«é¢å¿ãæãããã®ä»ã®åœæ¥è ã«ãã€ãŠæ±ºå®ãã
ããããªå²åã§éåžžäŸãã°æ¶è²»ããã飌æã®ïŒã
50mg/Kgãè¡šãããããªå²åã§åç©ããã³é³¥ã«æ
äžããããå ã«è¿°ã¹ãããã«ãååç©ã¯é£²ææ°ŽãŸ
ãã¯åºäœãŸãã¯ååºäœã®é£Œæã«ãã€ãŠæäžããŠã
ããã ãã¬ãããŸãã¯é¡ç²ã®åœ¢æ ã®æ¿åç©ãäžè¬åŒ
ã®æŽ»æ§ååç©ã®æäžæ段ãšããŠçšããå Žåããã¬
ãããŸãã¯é¡ç²ããèªäœã«ååšãã掻æ§ååç©ã®
å²åã¯é£Œæäžã«é©ããäžèšã®å²åããããªãé«
ãããããŠæ¿åç©ã飌æå šäœã®å¹³åã§é£œæïŒKgåœ
ãïŒã50mgã®æŽ»æ§ååç©ã®éãäžããããã«é£Œæ
äžã«ååžã§ããããšãç解ãããã§ãããã 以äžã®äŸã¯æ¬çºæã«ããçé·ä¿é²çµæç©ã説æ
ãããã®ã§ããã äŸ 20 è±çšé£Œæçµæç© å€§éºŠ 71Kg ãšããããã 10Kg 倧è±ã±ãŒãïŒèçœè³ª50ïŒ å«æïŒ 15Kg ãã¿ãã³å«æããã©ã«ååç© ïŒKg ã¹ãã©ãã€ã·ã³ã®2â²âãšãã«ã°ã«ã¿ã¬ãŒã ïŒïœ
Claims (1)
- ãç¹èš±è«æ±ã®ç¯å²ã ïŒ äžè¬åŒïŒïŒ ãåŒäžãR1ã¯ã¢ã»ãã«åºãŸãã¯ããããªãã«åºãŸ
ãã¯æ°ŽçŽ ååãè¡šãããã¯ãã¿ãžãšãã¬ã³åº
ïŒïŒ¡äžã«ååšããäºéçµåã¯ãã©ã³ã¹ã§ããïŒã
è¡šããããããŠR2ã¯äžè¬åŒ ïœåŒäžãã¯ïŒãïŒåã®ççŽ ååãæããïŒäŸ¡ã®
飜åãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢ã«ãã¬
ã³åºãè¡šãããã¯ãããååãŸãã¯é žçŽ ååã
è¡šããããããŠR3ã¯ïŒãïŒåã®ççŽ ååãæã
ãçŽéãŸãã¯æåãéã¢ã«ãã«åºãïŒãïŒåã®ç
çŽ ååãæããã·ã¯ãã¢ã«ãã«åºãããšãã«åºãŸ
ãã¯ããšãã«ã¢ã«ãã«åºïŒã¢ã«ãã¬ã³éšåã¯çŽé
ãŸãã¯æåãéã§ãããããŠïŒãïŒåã®ççŽ åå
ãæããïŒã§ããïœãè¡šããããæããã¹ãã©ã
ã€ã·ã³èªå°äœã ïŒ åèšç¬¬ïŒé ã«ç€ºããäžè¬åŒã®åºã«ãããŠ
R3ãããšãã«ä»¥å€ã®ãã®ã§ãããåèšç¬¬ïŒé èš
èŒã®ã¹ãã©ãã€ã·ã³èªå°äœã ïŒ R1ãæ°ŽçŽ ååãè¡šãããåèšç¬¬ïŒé ãŸãã¯
第ïŒé èšèŒã®ã¹ãã©ãã€ã·ã³èªå°äœã ïŒ R2ãåèšç¬¬ïŒé ã«ç€ºãããäžè¬åŒã®åºã
è¡šãããäžè¬åŒã«ãããŠïŒ¹ãé žçŽ ååãè¡šãã
ãããŠR3ãïŒãïŒåã®ççŽ ååãæããçŽéãŸ
ãã¯æåãéã®ã¢ã«ãã«åºãè¡šãããåèšç¬¬ïŒé
ãªãã第ïŒé ã®ããããäžã€ã«èšèŒã®ã¹ãã©ãã€
ã·ã³èªå°äœã ïŒ R2ãäžè¬åŒã®åºãè¡šãããäžè¬åŒã«ã
ããŠïŒºããšãã¬ã³ãŸãã¯ããªã¡ãã¬ã³åºãè¡šã
ãããé žçŽ ååãè¡šãããããŠR3ãïŒãïŒå
ã®ççŽ ååãæããçŽéã®ã¢ã«ãã«åºãè¡šããã
åèšç¬¬ïŒé ãªãã第ïŒé ã®ããããäžã€ã«èšèŒã®
ã¹ãã©ãã€ã·ã³èªå°äœã ïŒ R3ããšãã«ãè¡šããåèšç¬¬ïŒé èšèŒã®ã¹ã
ã©ãã€ã·ã³èªå°äœã ïŒ äžè¬åŒ X1COOR5 ïŒïŒ ïŒåŒäžãX1ã¯èçŽ ååãŸãã¯å¡©çŽ ååãè¡šããã
ããŠR5ã¯ïŒãïŒåã®ççŽ ååãæããã¢ã«ãã«
åºãè¡šããïŒãæããååç©ããã³é žå容äœã®å
åšäžã«ãäžè¬åŒ R3YH ïŒïŒ ãåŒäžãã¯ãããååãŸãã¯é žçŽ ååãè¡šã
ãããããŠR3ã¯ïŒãïŒåã®ççŽ ååãæããçŽ
éãŸãã¯æåãéã¢ã«ãã«åºãïŒãïŒåã®ççŽ å
åãæããã·ã¯ãã¢ã«ãã«åºãããšãã«åºãŸãã¯
ããšãã«ã¢ã«ãã«åºïŒã¢ã«ãã¬ã³éšåã¯çŽéãŸã
ã¯æåãéã§ãããããŠïŒãïŒåã®ççŽ ååãæ
ããïŒãè¡šããããæããååç©ãšåå¿ãããã
ãšã«ãã€ãŠãäžè¬åŒ ãåŒäžãR1ã¯ã¢ã»ãã«åºãŸãã¯ããããªãã«åºãŸ
ãã¯æ°ŽçŽ ååãè¡šãããã¯ãã¿ãžãšãã¬ã³åº
ïŒïŒ¡äžã«ååšããäºéçµåã¯ãã©ã³ã¹ã§ããïŒã
è¡šããããããŠR4ã¯äžè¬åŒ ïŒåŒäžãã¯ïŒãïŒåã®ççŽ ååãæããïŒäŸ¡ã®
飜åãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢ã«ãã¬
ã³åºãè¡šããïŒã®åºãè¡šããããæããååç©ã
ããäžè¬åŒïŒïŒ ãåŒäžãR1ã¯ã¢ã»ãã«åºãŸãã¯ããããªãã«åºãŸ
ãã¯æ°ŽçŽ ååãè¡šãããã¯ãã¿ãžãšãã¬ã³åº
ïŒïŒ¡äžã«ååšããäºéçµåã¯ãã©ã³ã¹ã§ããïŒã
è¡šããããããŠR2ã¯äžè¬åŒ ïœåŒäžãã¯ïŒãïŒåã®ççŽ ååãæããïŒäŸ¡ã®
飜åãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢ã«ãã¬
ã³åºãè¡šãããã¯ãããååãŸãã¯é žçŽ ååã
è¡šããããããŠR3ã¯ïŒãïŒåã®ççŽ ååãæã
ãçŽéãŸãã¯æåãéã¢ã«ãã«åºãïŒãïŒåã®ç
çŽ ååãæããã·ã¯ãã¢ã«ãã«åºãããšãã«åºãŸ
ãã¯ããšãã«ã¢ã«ãã«åºïŒã¢ã«ãã¬ã³éšåã¯çŽé
ãŸãã¯æåãéã§ãããããŠïŒãïŒåã®ççŽ åå
ãæããïŒã§ããïœãè¡šããããæããååç©ã
補é ããæ¹æ³ã ïŒ é žå容äœã第ïŒã¢ãã³ã§ãããåèšç¬¬ïŒé èš
èŒã®æ¹æ³ã ïŒ åå¿ãç¡æ°ŽäžæŽ»æ§ææ©æº¶å€ã®ååšäžã«â30â
ãªããïŒ30âã®æž©åºŠã§è¡ãªããããåèšç¬¬ïŒé ãŸ
ãã¯ç¬¬ïŒé èšèŒã®æ¹æ³ã ïŒïŒ äžè¬åŒ X2OCZCOYR3 ïŒïŒ ãX2ã¯èçŽ ååãŸãã¯å¡©çŽ ååãŸãã¯äžè¬åŒ âOCOOR5 ïŒïŒ ïŒåŒäžãR5ã¯ïŒãïŒåã®ççŽ ååãæããã¢ã«ã
ã«åºãè¡šãããã¯ïŒãïŒåã®ççŽ ååãæãã
ïŒäŸ¡ã®é£œåãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢
ã«ãã¬ã³åºãè¡šãããã¯ãããååãŸãã¯é žçŽ
ååãè¡šããããããŠR3ã¯ïŒãïŒåã®ççŽ åå
ãæããçŽéãŸãã¯æåãéã¢ã«ãã«åºãïŒãïŒ
åã®ççŽ ååãæããã·ã¯ãã¢ã«ãã«åºãããšã
ã«åºãŸãã¯ããšãã«ã¢ã«ãã«åºïŒã¢ã«ãã¬ã³éšå
ã¯çŽéãŸãã¯æåãéã§ãããããŠïŒãïŒåã®ç
çŽ ååãæããïŒãè¡šããããæããååç©ãšå
å¿ãããããšã«ãã€ãŠãäžè¬åŒ ãæããååç©ãããäžè¬åŒ ãåŒäžãR1ã¯ã¢ã»ãã«åºãŸãã¯ããããªãã«åºãŸ
ãã¯æ°ŽçŽ ååãè¡šãããã¯ãã¿ãžãšãã¬ã³åº
ïŒïŒ¡äžã«ååšããäºéçµåã¯ãã©ã³ã¹ã§ããïŒã
è¡šããããããŠR2ã¯äžè¬åŒ ïœåŒäžãã¯ïŒãïŒåã®ççŽ ååãæããïŒäŸ¡ã®
飜åãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢ã«ãã¬
ã³åºãè¡šãããããããååãŸãã¯é žçŽ ååã
è¡šããããããŠR3ã¯ïŒãïŒåã®ççŽ ååãæã
ãçŽéãŸãã¯æåãéã¢ã«ãã«åºãïŒãïŒåã®ç
çŽ ååãæããã·ã¯ãã¢ã«ãã«åºãããšãã«åºãŸ
ãã¯ããšãã«ã¢ã«ãã«åºïŒã¢ã«ãã¬ã³éšåã¯çŽé
ãŸãã¯æåãéã§ãããããŠïŒãïŒåã®ççŽ åå
ãæããïŒã§ããïœãè¡šããããæããååç©ã
補é ããæ¹æ³ã ïŒïŒ åå¿ãé žå容äœãŸãã¯ç¡æ©å¡©åºã®ååšäžã«
è¡ãªãããåèšç¬¬ïŒïŒé èšèŒã®æ¹æ³ã ïŒïŒ åå¿ãç¡æ°ŽäžæŽ»æ§ææ©æº¶å€ã®ååšäžã«â30
ããªããïŒ30âã®æž©åºŠã§è¡ãªãããã第ïŒïŒé ãŸ
ãã¯ç¬¬ïŒïŒé èšèŒã®æ¹æ³ã ïŒïŒ åŸãããäžè¬åŒã®ååç©ãæ¢ç¥æ¹æ³ã®é©
çšãŸãã¯æ¡çšã«ãã€ãŠãã®å¡©ã«å€æããå·¥çšãåŒ
ç¶ããŠè¡ãªããåèšç¬¬ïŒãïŒïŒé ã®ããããäžã€
ã«èšèŒã®æ¹æ³ã ïŒïŒ äžè¬åŒïŒïŒ ãåŒäžãR1ã¯ã¢ã»ãã«åºãŸãã¯ããããªãã«åºãŸ
ãã¯æ°ŽçŽ ååãè¡šãããã¯ãã¿ãžãšãã¬ã³åº
ïŒïŒ¡äžã«ååšããäºéçµåã¯ãã©ã³ã¹ã§ããïŒã
è¡šããããããŠR2ã¯äžè¬åŒ ïœåŒäžãã¯ïŒãïŒåã®ççŽ ååãæããïŒäŸ¡ã®
飜åãŸãã¯äžé£œåã®çŽéãŸãã¯æåãéã¢ã«ãã¬
ã³åºãè¡šãããã¯ãããååãŸãã¯é žçŽ ååã
è¡šããããããŠR3ã¯ïŒãïŒåã®ççŽ ååãæã
ãçŽéãŸãã¯æåãéã¢ã«ãã«åºãïŒãïŒåã®ç
çŽ ååãæããã·ã¯ãã¢ã«ãã«åºãããšãã«åºãŸ
ãã¯ããšãã«ã¢ã«ãã«åºïŒã¢ã«ãã¬ã³éšåã¯çŽé
ãŸãã¯æåãéã§ãããããŠïŒãïŒåã®ççŽ åå
ãæããïŒã§ããïœãè¡šããããæããã¹ãã©ã
ã€ã·ã³èªå°äœã®å°ããšãïŒçš®ãŸãã¯ãã®éæ¯æ§é ž
ä»å å¡©ããã³ãããšçžå®¹æ§ã®è£œè¬çã«èš±å®¹ãåŸã
æ äœãŸãã¯å€ç®ãããªãã補è¬æçç©çµæç©ã
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB51257/76A GB1540397A (en) | 1976-12-08 | 1976-12-08 | Spiramycin esters |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5371092A JPS5371092A (en) | 1978-06-24 |
| JPS6131114B2 true JPS6131114B2 (ja) | 1986-07-17 |
Family
ID=10459281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP14579277A Granted JPS5371092A (en) | 1976-12-08 | 1977-12-06 | Spiramycine ester |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US4174391A (ja) |
| JP (1) | JPS5371092A (ja) |
| BE (1) | BE861603A (ja) |
| DE (1) | DE2754323A1 (ja) |
| FR (1) | FR2373558A1 (ja) |
| GB (1) | GB1540397A (ja) |
| NL (1) | NL7713320A (ja) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009539970A (ja) | 2006-06-12 | 2009-11-19 | ã©ã¢ãã ã¢ãã ãã«âã¢ãã ãŠãããŒã·ãã£ãŒ ãªãããã | çã®æ²»çæ¹æ³ |
| CN105456283B (zh) * | 2015-12-30 | 2018-08-28 | äžå±±å€§åŠ | 倧ç¯å é ¯ç±»æççŽ æå ¶è¯çšçåšå¶å€ææ足å£ç è¯ç©äžçåºçš |
| MX390755B (es) * | 2017-07-04 | 2025-03-21 | Shenyang Fuyang Pharmaceutical Tech Co Ltd | Uso de isovaleril espiramicina i, ii y/o iii en la fabricación de medicamentos para el tratamiento y/o la prevención de tumores, y el medicamento. |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB785191A (en) * | 1955-04-13 | 1957-10-23 | Rhone Poulenc Sa | Hydrogenated spiramycins |
| FR5537M (ja) * | 1966-01-06 | 1967-11-13 | ||
| GB796311A (en) * | 1955-12-01 | 1958-06-11 | Rhone Poulenc Sa | Improvements in or relating to esters of spiramycins |
| US3000786A (en) * | 1957-06-14 | 1961-09-19 | Ciba Pharm Prod Inc | Process for making tetrahydro-spiramycin a |
| DE2134085C3 (de) * | 1970-07-09 | 1974-07-18 | Kyowa Hakko Kogyo K.K., Tokio | Spiramycin-hydrazone |
-
1976
- 1976-12-08 GB GB51257/76A patent/GB1540397A/en not_active Expired
-
1977
- 1977-12-01 NL NL7713320A patent/NL7713320A/xx not_active Application Discontinuation
- 1977-12-06 DE DE19772754323 patent/DE2754323A1/de active Granted
- 1977-12-06 US US05/857,999 patent/US4174391A/en not_active Expired - Lifetime
- 1977-12-06 JP JP14579277A patent/JPS5371092A/ja active Granted
- 1977-12-07 BE BE183253A patent/BE861603A/xx not_active IP Right Cessation
- 1977-12-07 FR FR7736836A patent/FR2373558A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| FR2373558B1 (ja) | 1980-04-25 |
| US4174391A (en) | 1979-11-13 |
| JPS5371092A (en) | 1978-06-24 |
| BE861603A (fr) | 1978-06-07 |
| DE2754323C2 (ja) | 1988-01-21 |
| NL7713320A (nl) | 1978-06-12 |
| DE2754323A1 (de) | 1978-06-15 |
| FR2373558A1 (fr) | 1978-07-07 |
| GB1540397A (en) | 1979-02-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR930002509B1 (ko) | ê²°ì ì± ì¹Œì ìë몚ë€ìŽíž ì ë² | |
| KR100919725B1 (ko) | ê°êžì ì§ë³ ë° ê°ìŒ ì¹ë£ ë° ìë°©ì© ì¡°ì±ë¬Œ | |
| CN110072844B (zh) | çšäºæ²»ç溶è¡æŒæµ·å§èæç¡ç åç»ç»èææçååç© | |
| IE49069B1 (en) | Parasiticidal heterocyclic ether derivatives,processes for the manufacture thereof and compositions thereof | |
| CN117024367B (zh) | ååªè¿æ¥çååç·é ®æªç䟧è³çŽ è¡çç©ååºçšåå ¶è¯ç©ç»åç© | |
| EP0087921B1 (en) | 20-amino tylosin derivatives | |
| JPH07110874B2 (ja) | ãšãªã¹ããã€ã·ã³ïœèªå°äœ | |
| JPS6131114B2 (ja) | ||
| JPH0631301B2 (ja) | æ现èæ§ïŒâããªããœâïŒïœâã¢ãªã«ããã³ãããã«ã®ã«âïŒïœâã¢ã¶âïŒïœâãã¢ãšãªã¹ããã€ã·ã³ïŒ¡èªå°äœ | |
| GB1577545A (en) | Treatment of swine dysenter | |
| US4452784A (en) | C-23-Modified derivatives of DMT | |
| JPS5925769B2 (ja) | åç©è¬çµæç©ãªãã³ã«ãã®è£œæ³ | |
| US4205081A (en) | Composition and method for preventing or treating swine dysentery | |
| US20060166905A1 (en) | Aivlosin for the treatment of disease due to brachyspira pilosicoli or ornithobacterium rhinotracheale | |
| KR100266471B1 (ko) | íì묌ì§ll-e19020ê°ë§,ë°ìŽììì°ë°©ë² | |
| US4785078A (en) | Transformation product of thiosporamicin | |
| DK142061B (da) | Fremgangsmåde til fremstilling af antibiotikum A-28086-kompleks, dets komponenter faktor A, faktor B og faktor D eller salte eller estere deraf. | |
| JPS5923789B2 (ja) | æçç©è³ªïœâïŒïŒïŒïŒïŒçç£åŸ®çç© | |
| SI8511013A8 (sl) | Postopek pridobivanja kristalnega kalcijevega psevdomonata ali hidrata le-tega | |
| PL91696B1 (ja) | ||
| CH633300A5 (en) | Spiramycin esters | |
| JPS58107141A (ja) | 家çã®çºè²ä¿é²ã»é£Œæå¹çæ¹åå€ | |
| JPS6339596B2 (ja) | ||
| GB1573121A (en) | Deltamycin derivatives |