JPS61254184A - 高濃度連続培養方法及び装置 - Google Patents
高濃度連続培養方法及び装置Info
- Publication number
- JPS61254184A JPS61254184A JP60094161A JP9416185A JPS61254184A JP S61254184 A JPS61254184 A JP S61254184A JP 60094161 A JP60094161 A JP 60094161A JP 9416185 A JP9416185 A JP 9416185A JP S61254184 A JPS61254184 A JP S61254184A
- Authority
- JP
- Japan
- Prior art keywords
- culture
- pipe
- membranes
- supply side
- membrane
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title description 8
- 239000012528 membrane Substances 0.000 claims abstract description 71
- 230000002441 reversible effect Effects 0.000 claims abstract description 9
- 238000001914 filtration Methods 0.000 claims description 37
- 239000012466 permeate Substances 0.000 claims description 25
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 239000001963 growth medium Substances 0.000 claims description 15
- 239000002609 medium Substances 0.000 claims description 14
- 239000002207 metabolite Substances 0.000 claims description 8
- 238000012136 culture method Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 12
- 238000005406 washing Methods 0.000 abstract description 3
- 238000007599 discharging Methods 0.000 abstract 1
- 238000007796 conventional method Methods 0.000 description 20
- 238000011001 backwashing Methods 0.000 description 11
- 239000007788 liquid Substances 0.000 description 9
- 239000012530 fluid Substances 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 230000007423 decrease Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 229920001410 Microfiber Polymers 0.000 description 2
- 235000010323 ascorbic acid Nutrition 0.000 description 2
- 239000011668 ascorbic acid Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000002054 inoculum Substances 0.000 description 2
- 239000003658 microfiber Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 229940072107 ascorbate Drugs 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000012510 hollow fiber Substances 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60094161A JPS61254184A (ja) | 1985-05-01 | 1985-05-01 | 高濃度連続培養方法及び装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60094161A JPS61254184A (ja) | 1985-05-01 | 1985-05-01 | 高濃度連続培養方法及び装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61254184A true JPS61254184A (ja) | 1986-11-11 |
| JPS6363194B2 JPS6363194B2 (enrdf_load_stackoverflow) | 1988-12-06 |
Family
ID=14102644
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60094161A Granted JPS61254184A (ja) | 1985-05-01 | 1985-05-01 | 高濃度連続培養方法及び装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61254184A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02255079A (ja) * | 1989-03-29 | 1990-10-15 | Shimadzu Corp | 細胞培養装置 |
| US5627070A (en) * | 1995-07-26 | 1997-05-06 | Celltherapy, Inc. | Cell growing device for in vitro cell population expansion |
| JP2005261342A (ja) * | 2004-03-19 | 2005-09-29 | Yanmar Co Ltd | プランクトン培養システム |
-
1985
- 1985-05-01 JP JP60094161A patent/JPS61254184A/ja active Granted
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02255079A (ja) * | 1989-03-29 | 1990-10-15 | Shimadzu Corp | 細胞培養装置 |
| US5627070A (en) * | 1995-07-26 | 1997-05-06 | Celltherapy, Inc. | Cell growing device for in vitro cell population expansion |
| US5763261A (en) * | 1995-07-26 | 1998-06-09 | Celltherapy, Inc. | Cell growing device for in vitro cell population expansion |
| JP2005261342A (ja) * | 2004-03-19 | 2005-09-29 | Yanmar Co Ltd | プランクトン培養システム |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6363194B2 (enrdf_load_stackoverflow) | 1988-12-06 |
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