JPS6113952A - Blood conduit - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Technical Field of the Invention The present invention relates to a blood conduit that is characterized by having an amino acid polymer on its inner surface, thereby making it difficult for cells to adhere to it.

Blood conduits include artificial blood vessels, catheters, shunts (used to lead blood out of the body), dialysis-type artificial kidneys,
It includes the tubular blood flow path used in model artificial lungs, artificial hearts, etc.

(b) Description of the prior art Conventionally, blood conduits have been made using synthetic polymer materials such as silicone rubber, polyurethane, and tetrafluoroethylene. Cells adhere, ie, a blood clot forms, obstructing blood flow. Therefore, there is a need for materials that do not allow cells to adhere to them in blood conduits.

(C) Object of the Invention The object of the present invention is to solve the above problem by providing a blood conduit with less cell adhesion by using an amino acid polymer.

(d) Structure of the Invention The present inventor has repeatedly conducted various studies on materials that have properties that prevent cells from adhering to them, and has found that amino acid polymers have properties that do not allow cells to adhere to a large extent and are suitable for use as blood conduits. This discovery led to the completion of the present invention.

That is, the blood conduit of the present invention can be obtained by molding an amino acid polymer into a desired tubular shape, or by molding the amino acid polymer into a tubular shape in advance and then applying the amino acid polymer to the inner surface of the tubular shape. .

The amino acids used as the amino acid polymer material of the present invention are:
It may be 0-form, L-form, or racemic form, and the amino acids used include alanine, glycine, methionine, leucine, valine, glutamic acid derivatives, aspartic acid derivatives, lysine derivatives, ornithine derivatives, and the like. The molecular weight of the amino acid polymer may be such that it forms a film.

(e) Examples of the invention Next, the present invention will be explained in more detail with reference to Examples.

Example 1 A solution of benzyl glutamate homopolymer was cast on a glass plate and air-dried to obtain a film (0.05 mm in thickness). This film was subjected to Soxhlet extraction with ethanol and then dried.

Example 2 A film was obtained in the same manner as in Example 1, except that a dichloroethane solution of methyl glutamate homopolymer was used instead of the dioquine solution of benzyl glutamate homopolymer.

Example 3 A culture solution containing human-derived epithelial cells (approximately 100,000 cells/rl) was kept in contact with the film obtained in Example 1 and Example 2 at a carbon dioxide concentration of 5% and humidity of 100%. , and left in a room at 37°C. After 17 hours, the film was lightly washed with phosphate buffer, and the amount of cells adhering to the film was quantified by a staining method. For comparison, a similar cell adhesion test was conducted using silicone rubber used for blood conduits and a commercially available cell culture sheet as a standard sample. The cell attachment rate was determined by dividing the number of cells attached to the film by the amount of cells attached to the standard sample, and the results are shown in Table 1.

Table 1 Example 4 An amino acid polymer was coated on the inner surface of a glass tube, and after drying, it was removed from the glass tube to obtain a tubular product.

('') Effects of the Invention As explained above, the present invention suppresses cell adhesion by molding an amino acid polymer on the inner surface of a blood conduit that requires low cell adhesion. It is possible to obtain the conduit in any shape.

designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology Keiji Takahashi

Claims (1)

[Claims] (1) Blood conduit with amino acid polymer on its inner surface