CN108129687B - A kind of surface is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine - Google Patents

A kind of surface is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine Download PDF

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CN108129687B
CN108129687B CN201711395081.XA CN201711395081A CN108129687B CN 108129687 B CN108129687 B CN 108129687B CN 201711395081 A CN201711395081 A CN 201711395081A CN 108129687 B CN108129687 B CN 108129687B
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phosphorylcholine
membrane structure
coating
containing amino
layer membrane
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CN108129687A (en
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宫铭
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Shaanxi coalfield geology Group Co Ltd
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Shaanxi Coalfield Geology Group Co Ltd
Xian University of Science and Technology
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    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J7/00—Chemical treatment or coating of shaped articles made of macromolecular substances
    • C08J7/04—Coating
    • C08J7/042—Coating with two or more layers, where at least one layer of a composition contains a polymer binder
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B05—SPRAYING OR ATOMISING IN GENERAL; APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D—PROCESSES FOR APPLYING FLUENT MATERIALS TO SURFACES, IN GENERAL
    • B05D7/00—Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials
    • B05D7/24—Processes, other than flocking, specially adapted for applying liquids or other fluent materials to particular surfaces or for applying particular liquids or other fluent materials for applying particular liquids or other fluent materials
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/34—Esters containing nitrogen, e.g. N,N-dimethylaminoethyl (meth)acrylate
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
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    • C08F230/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal
    • C08F230/02—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing phosphorus, selenium, tellurium or a metal containing phosphorus
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
    • C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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    • C09D143/00—Coating compositions based on homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing boron, silicon, phosphorus, selenium, tellurium, or a metal; Coating compositions based on derivatives of such polymers
    • C09D143/02—Homopolymers or copolymers of monomers containing phosphorus
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
    • C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
    • C08J2443/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and containing boron, silicon, phosphorus, selenium, tellurium or a metal; Derivatives of such polymers
    • C08J2443/02—Homopolymers or copolymers of monomers containing phosphorus
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    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08K—Use of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K5/00—Use of organic ingredients
    • C08K5/04—Oxygen-containing compounds
    • C08K5/07—Aldehydes; Ketones

Abstract

The invention discloses a kind of preparation methods for the imitating cell outer-layer membrane structure coating that surface is Phosphorylcholine, vinyl monomer containing amphoteric ion Phosphorylcholine group, the vinyl monomer containing amino are passed through simple solution free radical polymerization by the present invention, synthesize the phosphoryl choline polymer containing amino, and it is dissolved in polar solvent with glutaraldehyde and is uniformly mixed, coated in the modified material surface of needs through handling, then it is grafted the phosphoryl choline polymer containing amino, can be obtained the high imitating cell outer-layer membrane structure coating of surface Phosphorylcholine density.This preparation method is simple, mild condition, and obtaining the excellent coating surface of blood compatibility to improve coating surface Phosphorylcholine density provides a kind of new approach.The modified material of this imitating cell outer-layer membrane structure, will be in blood purification, and material implanted, organizational project, the fields such as medicament slow release and biosensor have broad application prospects.

Description

A kind of surface is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine
Technical field
The invention belongs to material surface science and biological medical polymer material technical field, and in particular to a kind of surface is The preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine.
Background technique
Chitosan has many advantages, such as degradability, antibiotic property, nontoxic, non-stimulated, pH responsiveness (Carbohydrate Polymers 2010,79:724-730), it has been widely used in the fields such as biomedicine.More and more researches show that: Chitosan and its derivative material can be used for blood purification.Amino in chitosan molecule facilitates to toxin a variety of in blood Absorption, can be used for blood Absorbent (Chemical Journal of Chinese Universities 2002,23:75-77；Journal of Microencapsulation 1993,10:475-486).Chitosan film has high dialysance, selectivity and intensity, can be with As haemodialysis material (Journal of Applied Polymer Science 1992,46:255-261；263-269). Although chitosan and its derivative has many advantages as blood purification material, there is also protein absorption, and blood is small The problems such as plate sticks, and eventually leads to blood coagulation, forms thrombus, so the blood compatibility for improving chitosan and its derivative material is compeled In the eyebrows and eyelashes (Applied Surface Science 2005,241:485-492；Biomaterials 2002,23:2561- 2568；Biomaterials 2003,24:3213-3220).
Phosphorylcholine (phosphorylcholine, PC) is the terminal hydrophyllic group for forming cell membrane basic unit lecithin, is Outer layer functional group in extracellular tunic, simultaneous with positive and negative xenogenesis charge, ability and hydrophily with stronger combination water Can, the surface of this structure and composition will not only be adsorbed with physiological environment interaction and depositing proteins, will not cause Platelet activation leads to the adverse reactions such as blood coagulation, has good biocompatibility.In recent years studies have shown that using phosphinylidyne Choline group and its polymer construct on the surface of the material has imitating cell outer-layer membrane structure, can significantly improve the blood phase of material Capacitive.
In recent years, using approach (the Carbohydrate Polymers 2007,70:82- of grafting Phosphorylcholine small molecule 88；Biomacromolecules 2007,8:3169-3176；Biomacromolecules 2006,7:3151-3156； Journal of Applied Polymer Science 2003,88:489-493；Polymer International 2003,52:81-85；Journal of biomaterials science,Polymer edition 2002,13:501- 510；Colloids and Surfaces B:Biointerfaces 2009,71:268-274) modification of chitosan, so that shell is poly- The blood compatibility of sugar significantly improves.But the density of the Phosphorylcholine group of these modes often on the surface of the material is not high, limit It has been made in the modified application in field of bio-medical material and further increasing for blood compatibility.
For this purpose, by the methacrylic acid containing Phosphorylcholine group-methylacryoyloxyethyl Phosphorylcholine binary copolymerization Object (PMA) polyanion carries out layer upon layer electrostatic self assembly with chitosan (polycation), obtains with imitating cell outer-layer film knot The coating surface (Colloids and Surfaces B:Biointerfaces 2011,85:48-55) of structure.Protein absorption With platelet adhesion reaction the results showed that the blood compatibility of modified rear surface is obviously improved.In view of this modification side The all the advantages of method will provide technical support to promote the blood compatibility of bio-medical material.However, with physical absorption side Formula is incorporated in the polymer with simulated cellulosa membrane structure coating of transplanting device surface, inevitably occurs in complex environment in vivo molten It solves, fall off.For this purpose, Lewis and Xu Jian equality (Biomaterials 2001,22:99-111；Biomaterials 2004, 25:3099-3108；European Polymer Journal 2004,40:291-298) respectively to contain trimethoxy silicon substrate The polymer coating of group and Phosphorylcholine group is studied.The result shows that trimethoxy on polymer molecular chain in coating Silicon group, which meets water, can occur hydrolysis, crosslinking, covalent bond can also be formed with the active group of substrate surface, to make Phosphorylcholine class The performance of polymer coating is significantly improved.It can be seen that between polymer crosslinking and its with substrate surface functional group Reaction is the key factor for improving Phosphorylcholine quasi polymer coating performance.
Crosslinkable groups, which are introduced into phosphoryl choline polymer, can form metastable imitating cell outer-layer membrane structure coating, But the phosphoryl choline polymer of this building stable coatings synthesizes complexity, using relatively difficult, stability is undesirable.In addition, by The limitation of Phosphorylcholine content and coating be dry in polymer, migration in the Phosphorylcholine group orientation of surface during storage, causes Keep coating surface Phosphorylcholine groups density lower, be not easy effective simulation cell outer-layer membrane structure, blood compatibility need It improves.Therefore, constructing the high imitating cell outer-layer membrane structure coating of stable structure, Phosphorylcholine groups density has important research Meaning and application prospect.
Summary of the invention
It is phosphinylidyne technical problem to be solved by the present invention lies in a kind of surface in view of the above shortcomings of the prior art, is provided The preparation method of the imitating cell outer-layer membrane structure coating of choline.By the vinyl monomer containing amphoteric ion Phosphorylcholine group, Containing amido vinyl monomer by simple solution free radical polymerization, the phosphoryl choline polymer containing amino is synthesized, and will It is dissolved in polar solvent with glutaraldehyde and is uniformly mixed, and coated in chitosan film surface, dries, and is then coated with the phosphinylidyne containing amino Choline polymer solution.The phosphoryl choline polymer containing amino is coated to the polymerization of the Phosphorylcholine containing amino by coating Object and glutaraldehyde coating surface, then amino and chitosan film surface amino groups and penta 2 in phosphoryl choline polymer are controlled by temperature Aldehyde reaction is anchored substrate while being crosslinked coat inside, make remaining phosphinylidyne gallbladder of the aldehyde radical grafting containing amino of coating surface Alkali polymer can be obtained surface phosphinylidyne gallbladder so that the thickness of coating reduces the density for significantly improving coating surface Phosphorylcholine The high imitating cell outer-layer membrane structure coating of alkali density.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A kind of surface is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine, comprising the following steps:
Step 1: the vinyl monomer containing amphoteric ion Phosphorylcholine group, the vinyl monomer containing amino are led to Solution free radical polymerization is crossed, the phosphoryl choline polymer containing amino is synthesized；
It is uniformly mixed, coats Step 2: the phosphoryl choline polymer containing amino is dissolved in polar solvent with glutaraldehyde Modified material surface is being needed, is being dried；
Step 3: by the property material surface after being dried in the phosphoryl choline polymer solution coating step two containing amino；
Step 4: the film handled in step 3 is placed in distilled water, handled under the conditions of 80 DEG C~95 DEG C 2h~ 12h；It is washed with solvent, obtains the high imitating cell outer-layer membrane structure coating of surface Phosphorylcholine density.
The vinyl monomer containing amphoteric ion Phosphorylcholine group is 2- methylacryoyloxyethyl Phosphorylcholine Monomer, the vinyl monomer containing amino are 2- aminoethyl methacrylate hydrochloric acid salt monomer.
In step 1, the vinyl monomer containing amphoteric ion Phosphorylcholine group and the vinyl list containing amino Body molar ratio is about (90:10)~(70:30).
In step 2, the polar solvent is methanol or ethyl alcohol.
In step 2, the phosphoryl choline polymer containing amino and glutaraldehyde are dissolved in polar solvent, the phosphorus containing amino The concentration of phatidylcholine polymer is about 0.5~5mg/mL.
In step 2,10 microlitres/cm of volume is coated2/ face.
In two, the molar ratio of aldehyde radical is about 100 in the amino and glutaraldehyde in the phosphoryl choline polymer containing amino: (5 ~20).
In step 2, needing modified material is chitosan material.
In step 3, the concentration of the phosphoryl choline polymer solution containing amino of coating is 1~5mg/mL, and solvent is first Alcohol coats 5~50 microlitres/cm of volume2/ face.
In step 4, solvent washing is successively with methanol, distillation water washing.
Compared with the prior art, the present invention has the following advantages:
Amino-containing phosphoryl choline polymer of the invention is by the vinyl monomer containing Phosphorylcholine and to contain ammonia The vinyl monomer of base uses the binary randomcopolymer of radical polymerization synthesis, which mixes with glutaraldehyde It is even, it coated in chitosan film surface, dries, is then coated with the phosphoryl choline polymer solution containing amino.It will be contained by coating There is the phosphoryl choline polymer of amino to be coated to the phosphoryl choline polymer containing amino and glutaraldehyde coating surface, then passes through temperature Amino and chitosan film surface amino groups are reacted with glutaraldehyde in degree control phosphoryl choline polymer, while being crosslinked coat inside It is anchored substrate, makes remaining phosphoryl choline polymer of the aldehyde radical grafting containing amino of coating surface, so that the thickness of coating reduces The density for significantly improving coating surface Phosphorylcholine can be obtained the high imitating cell outer-layer membrane structure of surface Phosphorylcholine density and apply Layer.The preparation method of the high imitating cell outer-layer membrane structure coating of stabilization of the invention, Phosphorylcholine groups density is simple, condition temperature With provide a kind of new approach to obtain the coating surface of imitating cell outer-layer membrane structure haveing excellent performance；The present invention utilizes painting The remaining aldehyde radical of coating surface is covered, the phosphoryl choline polymer containing amino is grafted, improves coating surface Phosphorylcholine group Density significantly improves the performance of coating.The high imitating cell outer-layer membrane structure coating of stabilization, the Phosphorylcholine groups density of preparation will It is material implanted in blood purification, organizational project, before the fields such as medicament slow release and biosensor have wide application Scape.
Detailed description of the invention
Fig. 1 is the dynamic contact angle of chitosan film of the present invention (CS) and Chitosan film (CS-PMH20GA-PMH10).
Fig. 2 is chitosan film of the present invention (CS) and the surface-element of Chitosan film (CS-PMH20GA-PMH10) is fine Energy spectrum diagram.
Fig. 3 is chitosan film of the present invention (CS) and the AFM figure of Chitosan film (CS-PMH20GA-PMH10).
Fig. 4 is chitosan film of the present invention (CS) and the fluorescin absorption of Chitosan film (CS-PMH20GA-PMH10) Amount.
Specific embodiment
With reference to the accompanying drawings and examples, technical solution of the present invention is described in further detail.
Embodiment 1
Weigh 16mmol 2- methylacryoyloxyethyl Phosphorylcholine and 4mmol 2- aminoethyl methacrylate hydrochloric acid Salt, using 0.1mmol potassium peroxydisulfate as initiator, under nitrogen protection, 70 DEG C of polymerization reactions for 24 hours, are dialysed, then after reaction It is freeze-dried at -50 DEG C, obtains amino-containing phosphoryl choline polymer PMH20 (amino in 20 representation polymer synthesis processes Vinyl monomer molar ratio be 20%).The samples such as PMH10, PMH15 and PMH25 can similarly be synthesized.
With 400MHz Nuclear Magnetic Resonance with D2O is the proton magnetic of solvent test polymer.Appearance is had no at 5~7ppm, Show there is no residual monomer in gained copolymer, and successfully synthesize the polymer, at 3.28ppm for-N+(CH3)3Feature Peak calculates polymer composition for methylene on main chain and the peak of pendant methyl at 0.9~2.2ppm, it is known that polymer composition with Feed ratio is almost the same.
Phosphoryl choline polymer containing amino and glutaraldehyde are dissolved in methanol by amino and aldehyde radical molar ratio for 100: 10 In solvent after mixing, make the concentration 1.0mg/mL of the phosphoryl choline polymer containing amino, coat 10 microlitres/cm2/ face It is dried on the surface chitosan material (CS), then through 90 degree of processing 6h in distilled water, later successively with a large amount of methanol, distilled water Washing, can obtain contrast sample (CS-PMH20/GA), then by 10 microlitres of volume of the methanol solution of 1mg/mL PMH10 coating/ cm2/ face, dries, after through 90 degree of processing 6h in distilled water, later successively with a large amount of methanol, distillation water washing, can be obtained The coating (CS-PMH20/GA-PMH10) of imitating cell outer-layer membrane structure.
It by amino and aldehyde radical molar ratio is 100: 10 by phosphoryl choline polymer and glutaraldehyde that the present embodiment contains amino The concentration 1.0mg/mL for making the phosphoryl choline polymer containing amino after mixing is dissolved in methanol solvate, and coating 10 is micro- Liter/cm2/ face is dried on chitosan material surface, and the methanol solution of 1mg/mL PMH10 is then coated 10 microlitres/cm of volume2/ Face is dried, after through 90 degree of processing 6h in distilled water, later successively with a large amount of methanol, distillation water washing, phosphinylidyne can be obtained The coating surface (CS-PMH20GA-PMH10) of the high imitating cell outer-layer membrane structure of content of choline.
As shown in Figure 1, chitosan material of the present embodiment through coating treatment and the chitosan material handled through control coatings It compares, the surface advancing angle of the chitosan through coating treatment is lower, this is because the phosphoryl choline polymer containing amino and penta The remaining aldehyde radical of dialdehyde coating surface is grafted the phosphoryl choline polymer containing amino by schiff base reaction and further increases painting The density of layer surface Phosphorylcholine group, so that coating surface hydrophily is higher, advancing angle is lower.
As shown in Figure 2 and Table 1, chitosan material of the present embodiment through coating treatment and the chitosan handled through control coatings Material is compared, N and P characteristic absorption peak is larger on the chitosan film surface Phosphorylcholine group of modified processing, surface phosphinylidyne gallbladder Base groups density is higher.
The surface-element content of 1. chitosan film of table (CS) and Chitosan film (CS-PMH20GA-PMH10)
As shown in figure 3, chitosan material of the present embodiment through coating treatment and the chitosan material handled through control coatings It compares, the surface topography of the chitosan through coating treatment is dramatically different.
As shown in figure 4, chitosan material of the present embodiment through coating treatment and the chitosan material handled through control coatings It compares, processed chitosan fluorescin adsorbance has apparent reduction, and blood compatibility significantly improves.
Embodiment 2
Weigh 17mmol 2- methylacryoyloxyethyl Phosphorylcholine and 3mmol 2- aminoethyl methacrylate hydrochloric acid Salt, using 0.1mmol potassium peroxydisulfate as initiator, under nitrogen protection, 70 DEG C of polymerization reactions for 24 hours, are dialysed, then after reaction It is freeze-dried at -50 DEG C, obtains amino-containing phosphoryl choline polymer.
It by amino and aldehyde radical molar ratio is 100: 5 molten by phosphoryl choline polymer and glutaraldehyde that the present embodiment contains amino In alcohol solvent after mixing, make the concentration 0.5mg/mL of the phosphoryl choline polymer containing amino, 10 microlitres of coating/ cm2/ face is dried on chitosan material surface, and the methanol solution of 2mg/mL PMH20 is then coated 5 microlitres/cm of volume2/ face, dries in the air It is dry, after through 80 degree processing 12h in distilled water, later successively with a large amount of methanol, distill water washing, Phosphorylcholine can be obtained The coating surface of the high imitating cell outer-layer membrane structure of content.
Embodiment 3
Weigh 12mmol 2- methylacryoyloxyethyl Phosphorylcholine and 8mmol 2- aminoethyl methacrylate hydrochloric acid Salt, using 0.1mmol potassium peroxydisulfate as initiator, under nitrogen protection, 70 DEG C of polymerization reactions for 24 hours, are dialysed, then after reaction It is freeze-dried at -50 DEG C, obtains amino-containing phosphoryl choline polymer.
It by amino and aldehyde radical molar ratio is 100: 15 by phosphoryl choline polymer and glutaraldehyde that the present embodiment contains amino The concentration 2mg/mL for making the phosphoryl choline polymer containing amino after mixing is dissolved in methanol solvate, 10 microlitres of coating/ cm2/ face is dried on chitosan material surface, and the methanol solution of 3mg/mL PMH15 is then coated 20 microlitres/cm of volume2/ face, Dry, after through 85 degree of processing 11h in distilled water, later successively with a large amount of methanol, distillation water washing, phosphinylidyne gallbladder can be obtained The coating surface of the high imitating cell outer-layer membrane structure of alkali content.
Embodiment 4
Weigh 15mmol 2- methylacryoyloxyethyl Phosphorylcholine and 5mmol 2- aminoethyl methacrylate hydrochloric acid Salt, using 0.1mmol potassium peroxydisulfate as initiator, under nitrogen protection, 70 DEG C of polymerization reactions for 24 hours, are dialysed, then after reaction It is freeze-dried at -50 DEG C, obtains amino-containing phosphoryl choline polymer.
It by amino and aldehyde radical molar ratio is 100: 20 by phosphoryl choline polymer and glutaraldehyde that the present embodiment contains amino The concentration 3mg/mL for making the phosphoryl choline polymer containing amino after mixing is dissolved in alcohol solvent, 10 microlitres of coating/ cm2/ face is dried on chitosan material surface, and the methanol solution of 4mg/mL PMH10 is then coated 30 microlitres/cm of volume2/ face, Dry, after through 95 degree of processing 2h in distilled water, later successively with a large amount of methanol, distillation water washing, phosphinylidyne gallbladder can be obtained The coating surface of the high imitating cell outer-layer membrane structure of alkali content.
Embodiment 5
Weigh 14mmol 2- methylacryoyloxyethyl Phosphorylcholine and 6mmol 2- aminoethyl methacrylate hydrochloric acid Salt, using 0.1mmol potassium peroxydisulfate as initiator, under nitrogen protection, 70 DEG C of polymerization reactions for 24 hours, are dialysed, then after reaction It is freeze-dried at -50 DEG C, obtains amino-containing phosphoryl choline polymer.
It by amino and aldehyde radical molar ratio is 100: 12 by phosphoryl choline polymer and glutaraldehyde that the present embodiment contains amino The concentration 4mg/mL for making the phosphoryl choline polymer containing amino after mixing is dissolved in methanol solvate, 10 microlitres of coating/ cm2/ face is dried on chitosan material surface, and the methanol solution of 5mg/mL PMH15 is then coated 40 microlitres/cm of volume2/ face, Dry, after through 83 degree of processing 11h in distilled water, later successively with a large amount of methanol, distillation water washing, phosphinylidyne gallbladder can be obtained The coating surface of the high imitating cell outer-layer membrane structure of alkali content.
Embodiment 6
Weigh 13mmol 2- methylacryoyloxyethyl Phosphorylcholine and 7mmol 2- aminoethyl methacrylate hydrochloric acid Salt, using 0.1mmol potassium peroxydisulfate as initiator, under nitrogen protection, 70 DEG C of polymerization reactions for 24 hours, are dialysed, then after reaction It is freeze-dried at -50 DEG C, obtains amino-containing phosphoryl choline polymer.
It by amino and aldehyde radical molar ratio is 100: 7 molten by phosphoryl choline polymer and glutaraldehyde that the present embodiment contains amino In alcohol solvent after mixing, make the concentration 1.5mg/mL of the phosphoryl choline polymer containing amino, 10 microlitres of coating/ cm2/ face is dried on chitosan material surface, and the methanol solution of 1.5mg/mL PMH20 is then coated 50 microlitres/cm of volume2/ Face is dried, after through 87 degree of processing 8h in distilled water, later successively with a large amount of methanol, distillation water washing, phosphinylidyne can be obtained The coating surface of the high imitating cell outer-layer membrane structure of content of choline.
Embodiment 7
Weigh 16mmol 2- methylacryoyloxyethyl Phosphorylcholine and 4mmol 2- aminoethyl methacrylate hydrochloric acid Salt, using 0.1mmol potassium peroxydisulfate as initiator, under nitrogen protection, 70 DEG C of polymerization reactions for 24 hours, are dialysed, then after reaction It is freeze-dried at -50 DEG C, obtains amino-containing phosphoryl choline polymer.
It by amino and aldehyde radical molar ratio is 100: 17 by phosphoryl choline polymer and glutaraldehyde that the present embodiment contains amino The concentration 2.5mg/mL for making the phosphoryl choline polymer containing amino after mixing is dissolved in methanol solvate, and coating 10 is micro- Liter/cm2/ face is dried on chitosan material surface, then by 25 microlitres of volume of the methanol solution of 2.5mg/mL PMH10 coating/ cm2/ face, dries, after through 92 degree of processing 3h in distilled water, later successively with a large amount of methanol, distillation water washing, can be obtained The coating surface of the high imitating cell outer-layer membrane structure of Phosphorylcholine content.
The above is only presently preferred embodiments of the present invention, not does any restrictions to the present invention, all according to invention skill Art any simple modification substantially to the above embodiments, change and equivalent structural changes, still fall within the technology of the present invention In the protection scope of scheme.

Claims (7)

1. the preparation method that a kind of surface is the imitating cell outer-layer membrane structure coating of Phosphorylcholine, which is characterized in that including following Step:
Step 1: the vinyl monomer containing amphoteric ion Phosphorylcholine group, the vinyl monomer containing amino are passed through molten Liquid free radical polymerization synthesizes the phosphoryl choline polymer containing amino；
The vinyl monomer containing amphoteric ion Phosphorylcholine group is 2- methylacryoyloxyethyl Phosphorylcholine monomer, The vinyl monomer containing amino is 2- aminoethyl methacrylate hydrochloric acid salt monomer；
The vinyl monomer containing amphoteric ion Phosphorylcholine group is with the vinyl monomer molar ratio containing amino (90:10)~(70:30)；
It is uniformly mixed Step 2: the phosphoryl choline polymer containing amino is dissolved in polar solvent with glutaraldehyde, being coated in needs The material surface to be modified, dries；
Phosphoryl choline polymer containing amino and glutaraldehyde are dissolved in polar solvent, the phosphoryl choline polymer containing amino Concentration is 0.5~5mg/mL；
Step 3: by the modified material surface after being dried in the phosphoryl choline polymer solution coating step two containing amino；
Step 4: the film handled in step 3 is placed in distilled water, 2h~12h is handled under the conditions of 80 DEG C~95 DEG C； It is washed with solvent, obtains the high imitating cell outer-layer membrane structure coating of surface Phosphorylcholine density.
2. a kind of surface according to claim 1 is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine, It is characterized in that, the polar solvent is methanol or ethyl alcohol in step 2.
3. a kind of surface according to claim 1 is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine, It is characterized in that, coating 10 microlitres/cm of volume in step 22/ face.
4. a kind of surface according to claim 1 is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine, It is characterized in that, the molar ratio of aldehyde radical is in the amino and glutaraldehyde in the phosphoryl choline polymer containing amino in step 2 100: (5~20).
5. a kind of surface according to claim 1 is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine, It is characterized in that, needing modified material is chitosan material in step 2.
6. a kind of surface according to claim 1 is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine, It is characterized in that, the concentration of the phosphoryl choline polymer solution containing amino of coating is 1~5mg/mL, solvent in step 3 For methanol, 5~50 microlitres/cm of volume is coated2/ face.
7. a kind of surface according to claim 1 is the preparation method of the imitating cell outer-layer membrane structure coating of Phosphorylcholine, It is characterized in that, solvent washing is successively with methanol, distillation water washing in step 4.
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