JPS6150562A - Blood conduit - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

Detailed Description of the Invention (a) Technical Field of the Invention The present invention relates to a specialized blood tube that is characterized by having a polyglutamic acid derivative surface-treated with amino alcohol on its inner surface to prevent cells from adhering to it. It is.

Blood conduits include artificial blood vessels, catheters, shunts (used to lead blood out of the body), dialysis-type artificial kidneys,
It includes the tubular blood flow path used in model artificial lungs, artificial hearts, etc.

(b) Description of the Prior Art Conventionally, blood conduits are made of silicone rubber, polyurethane,
Blood conduits are made using synthetic polymer materials such as polytetrafluoroethylene, but in blood conduits made of these materials, cells adhere to the surface of the material, that is, thrombus forms, which obstructs blood flow. Therefore, in the blood conduit, 1
There is a need for materials that do not have single cells attached to them.

(C) Object of the Invention The object of the present invention is to solve the above-mentioned problem by providing a blood tube with less cell adhesion by using a polyglutamic acid derivative surface-treated with amino alcohol.

(d) Structure of the Invention The present inventor has repeatedly conducted various studies on materials that have properties that prevent cells from being easily damaged, and has found that polyglutamine Lg 19, whose surface has been treated with amino alcohol, has properties that do not significantly allow cells to adhere to it. The present inventors have discovered that this material is suitable for use as a blood conduit, and have completed the present invention.

゛ That is, the blood conduit of the present invention is made by molding a polyglutamic acid derivative into a desired tube shape, and then surface-treating the tube with amino alcohol and using IG, or by pre-shaping it into a tube shape with other polymeric materials. After that, a polyglutamic acid derivative is applied to the inner surface, and then the surface is treated with amino alcohol (q).

The polyglutamic acid derivative of the present invention may be 0-form, L-form, or racemic form. As the polyglutamic acid derivative, esters of aliphatic hydrocarbons and aromatic hydrocarbons such as methyl polyglutamine and benzyl polyglutamate are used. The number of molecules m of the polyglutamine IH conductor may be such that a film thereof is formed.

As amine alcohol, in addition to ethanolamine, aminopropanol, etc., Hz NC2H40C2HaOH
Alkylene glycol derivatives such as these are used.

The surface treatment time is appropriately selected depending on the type and concentration of the amino alcohol and the type of polyglutamic acid derivative, but in any case, the surface treatment should reduce the contact angle of the inner surface of the tube to water to 45 degrees or less. desirable.

(e) Examples of the invention Next, the present invention will be explained in more detail with reference to Examples.

Example 1 A solution of benzyl glutamate homopolymer in dioxane was cast on a glass plate, air-dried, and the result was a peel I15! (film thickness 0.0
5 mm> was obtained. This film was subjected to Soxhlet extraction with ethanol and then dried for 92 hours.

This film was heated at 55°C in 3-amino-1-propanol for 4 hours.
The amithrisis reaction was allowed to take some time. After the reaction, the film was washed with ethanol. This film was immersed in hot water of 80° C. or higher for 8 hours or more and dried to form a surface-treated film. Table 1 shows the contact angle of this surface-treated film with water.

Table 1 Example 2 A surface treated film was obtained in the same manner as in Example 1 except that a dichloroethane solution of methyl glutamate homopolymer was used instead of the dioxane solution of benzyl glutamate homopolymer.

Example 3 A culture solution containing human-derived epithelial cells (approximately 100,000 cells/mQ) was placed on the film obtained in Example 1 at 15% carbon dioxide concentration, 100% humidity, and 37°C. I left it in the room. After 11 hours, the film was gently washed with phosphate buffer, and the number of cell nails adhering to the film was quantified by nuclear staining. For comparison, we used silicone rubber used for blood conduits and a commercially available cell culture sheet as an 8X quasi-sample.
A similar cell adhesion test was conducted. m of cells attached to the membrane
The cell adhesion rate was determined by dividing (=J) by m of cells attached to the standard sample, and the results are not shown in Table 2.

Table 2 Example 4 H2NC2H40C2Ha instead of 4 hours treatment at 55°C in 3-amino-1-propatur 60°C in OH
A surface treated film was obtained in the same manner as in Example 1 except that the treatment was carried out for 4 hours.

Example 5 After applying polyglutamic acid derivative (9) to the inner surface of a glass tube and drying,
After being treated at 5°C for 4 hours and washed with ethanol, it was further immersed in hot water at 80°C or higher for 8 hours or more, and after drying, it was removed from the glass tube to obtain a tubular product.

(f) Effects of the Invention As explained above, the present invention is made by molding polyglutamine lLi, which has been surface-treated with amino alcohol, on the inner surface of a blood conduit that requires low cell adhesion. It is possible to suppress the adhesion of cells and to obtain this blood conduit in any shape.

designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology Kyouji Takahashi

Claims (1)

[Claims] (1) Blood conduit with polyglutamic acid derivative surface treated with amino alcohol on the inner surface