JPS6113953A - Blood conduit - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

DETAILED DESCRIPTION OF THE INVENTION (a) Technical Field of the Invention The present invention relates to a blood conduit that is characterized by having an amino acid polymer containing leucine on its inner surface, thereby making it difficult for cells to adhere to it.

Blood vessels include artificial blood vessels, catheters, shunts (used to lead blood out of the body), dialysis-type artificial kidneys,
It includes tubular blood flow channels used in molded artificial lungs, artificial hearts, etc.

(b') Description of Prior Art Conventionally, blood conduits have been made using synthetic polymer materials such as silicone rubber, polyurethane, and tetrafluoroethylene. Cells attach to the bloodstream, forming a blood clot, which obstructs blood flow. Therefore, there is a need for materials that do not allow cells to adhere to them in blood conduits.

(C) Object of the Invention The present invention aims to solve the above problem by providing a blood conduit with less cell adhesion by using an amino acid polymer containing leucine.

(d) Structure of the Invention The present inventor has repeatedly conducted various studies on materials that have properties that make it difficult for cells to adhere to them, and has found that amino acid polymers containing leucine have properties that do not allow cells to adhere to a large extent.
They found that it is suitable as a blood conduit and completed the present invention.

That is, the blood conduit of the present invention can be obtained by molding a leucine-containing amino acid polymer into a desired tube shape, or it can be obtained by molding an amino acid polymer containing leucine into a tube shape in advance, or it can be obtained by molding an amino acid polymer containing leucine into a tube shape in advance, and then molding the leucine-containing amino acid polymer onto the inner surface of the tube. Obtained by applying coalescence.

Leucine as the constituent material of the amino acid polymer of the present invention and the amino acid as its copolymerization component may be 0-form, L-form, or racemic form, and other amino acids include alanine, glycine, methionine, valine, glutamic acid derivatives, and aspartic acid. Derivatives, lysine derivatives, ornidine derivatives, etc. are used.

The molecular weight of the amino acid polymer is sufficient as long as it forms a film, and the leucine content in the polymer varies depending on the type of amino acid, but is preferably 30 mol% or more.

(e) Examples of the invention Next, the present invention will be roughly explained in detail with reference to Examples.

Example 1 Copolymer of L-leucine and benzyl L-glutamate (molar ratio of leucine to benzyl glutamate -7 = 3
) A mixed solution of benzene and dioxane (7:3) was cast on a glass plate and air-dried to form a film (0.05 mm thick).
) was obtained. This film was subjected to Soxhlet extraction with ethanol and then dried.

On this film, a culture solution containing human-derived epithelial cells (approximately 1
00,000 pieces/mΩ) in contact with 5% carbon dioxide gas,
It was left standing in a room with a humidity of 100% and a temperature of 37°C.

After 17 hours, the film was lightly washed with phosphate buffer, and the amount of cells adhering to the film was quantified by nuclear staining. For comparison, a similar cell adhesion test was conducted using a benzyl glutamate homopolymer film, silicone rubber used for blood conduits, and a commercially available cell culture sheet as a standard sample. The cell attachment rate was determined by dividing the amount of cells attached to the film by the amount of cells attached to the standard sample.

The results are shown in Table 1.

Table 1 Example 2 Instead of the copolymer of L-leucine and benzyl L-glutamate, a copolymer of L-leucine and monocarbobenzoxylidine (molar ratio of leucine to carbobenzoxylidine -7) was used. :3) A film was molded in the same manner as in Example 1, except that 3) was used, and a cell adhesion experiment was conducted. Second result
Shown in the table.

Table 2 Example 3 A leucine-containing amino acid polymer was applied to the inner surface of a glass tube, and after drying, it was removed from the glass tube, and the tube was weighed 1 q.

Example 4 In a copolymer of L-leucine and benzyl L-glutamate, the molar ratio of each is 85:15.50
: 50.30 : A film of 70 was obtained in the same manner as in Example 1.

Example 5 In a copolymer of L-leucine and L-carbobenzoxylidine, the molar ratio of each is 85:15.50:
A film of 50.30:70 was obtained in the same manner as in Example 1.

Example 6 Poly L-leucine was dissolved in hot benzene and a film was obtained in the same manner as in Example 1.

Example 7 A block copolymer consisting of L-leucine and benzyl L-glutamate was synthesized by the N-carboxyamino acid anhydride method (the composition of this block copolymer was (benzyl glutamate (18%))-veleucine (64%)
benzyl toheglutamate (18%)). )
. This copolymer was dissolved in hot benzene, and a film was obtained in the same manner as in Example 1.

Example 8 A block copolymer film was obtained in the same manner as in Example 7 except that L-carbobenzoxylidine was used instead of benzyl L-glutamate.

(f) Effects of the Invention As explained above, the present invention suppresses cell adhesion by molding an amino acid polymer containing leucine on the inner surface of a blood conduit that requires low cell adhesion. , it is possible to obtain this blood conduit in any shape.

designated agent Director, Product Science Research Institute, Agency of Industrial Science and Technology Keiji Takahashi 19λ3-

Claims (1)

[Claims] (1) Blood conduit with an amino acid polymer containing leucine on its inner surface